Kamceva, Tina

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  • Kamceva, Tina (8)

Author's Bibliography

Inhibitory effect of cisplatin and [Pt(dach)Cl2] on the activity of phospholipase A2

Radisavljevic, Maja; Kamceva, Tina; Bugarcic, Zivadin D.; Petković, Marijana

(2013)

TY  - JOUR
AU  - Radisavljevic, Maja
AU  - Kamceva, Tina
AU  - Bugarcic, Zivadin D.
AU  - Petković, Marijana
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5583
AB  - This work has been focused on testing the influence of two selected Pt(II) complexes cisplatin, Pt(NH3)(2) Cl-2, and [Pt(dach)Cl-2] on the activity of porcine pancreatic phospholipase A(2) (PLA(2)). It has been assumed that this enzyme plays a role in carcinogenesis and that it could be a target in the tumour therapy. The results of this study show that both Pt(II) complexes inhibit the activity of the enzyme, though they bind to it in a different manner. While cisplatin interacts with the enzyme in an acompetitive manner, the stable interaction of [Pt(dach)Cl-2] with PLA(2) could not be detected under our experimental conditions.
T2  - Journal of Enzyme Inhibition and Medicinal Chemistry
T1  - Inhibitory effect of cisplatin and [Pt(dach)Cl2] on the activity of phospholipase A2
VL  - 28
IS  - 4
SP  - 651
EP  - 660
DO  - 10.3109/14756366.2012.666539
ER  - 
@article{
author = "Radisavljevic, Maja and Kamceva, Tina and Bugarcic, Zivadin D. and Petković, Marijana",
year = "2013",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5583",
abstract = "This work has been focused on testing the influence of two selected Pt(II) complexes cisplatin, Pt(NH3)(2) Cl-2, and [Pt(dach)Cl-2] on the activity of porcine pancreatic phospholipase A(2) (PLA(2)). It has been assumed that this enzyme plays a role in carcinogenesis and that it could be a target in the tumour therapy. The results of this study show that both Pt(II) complexes inhibit the activity of the enzyme, though they bind to it in a different manner. While cisplatin interacts with the enzyme in an acompetitive manner, the stable interaction of [Pt(dach)Cl-2] with PLA(2) could not be detected under our experimental conditions.",
journal = "Journal of Enzyme Inhibition and Medicinal Chemistry",
title = "Inhibitory effect of cisplatin and [Pt(dach)Cl2] on the activity of phospholipase A2",
volume = "28",
number = "4",
pages = "651-660",
doi = "10.3109/14756366.2012.666539"
}
Radisavljevic, M., Kamceva, T., Bugarcic, Z. D.,& Petković, M. (2013). Inhibitory effect of cisplatin and [Pt(dach)Cl2] on the activity of phospholipase A2.
Journal of Enzyme Inhibition and Medicinal Chemistry, 28(4), 651-660.
https://doi.org/10.3109/14756366.2012.666539
Radisavljevic M, Kamceva T, Bugarcic ZD, Petković M. Inhibitory effect of cisplatin and [Pt(dach)Cl2] on the activity of phospholipase A2. Journal of Enzyme Inhibition and Medicinal Chemistry. 2013;28(4):651-660
Radisavljevic Maja, Kamceva Tina, Bugarcic Zivadin D., Petković Marijana, "Inhibitory effect of cisplatin and [Pt(dach)Cl2] on the activity of phospholipase A2" Journal of Enzyme Inhibition and Medicinal Chemistry, 28, no. 4 (2013):651-660,
https://doi.org/10.3109/14756366.2012.666539 .
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Sensitivity and accuracy of organic matrix-assisted Laser desorption and ionisation mass spectrometry of FeCl3 is higher than in in matrix-free approach

Radisavljevic, Maja; Kamceva, Tina; Vukićević, Iva A.; Nišavić, Marija; Milovanovic, Milan; Petković, Marijana

(2013)

TY  - JOUR
AU  - Radisavljevic, Maja
AU  - Kamceva, Tina
AU  - Vukićević, Iva A.
AU  - Nišavić, Marija
AU  - Milovanovic, Milan
AU  - Petković, Marijana
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5605
AB  - We compare the quality and reliability of laser desorption and ionisation mass spectra of FeCl3 acquired without the assistance of the matrix with the spectra acquired with different organic matrix molecules. Generally, inorganic salts tend to form clusters upon laser irradiation, the signals of which can be easily distinguished from ions arising from the matrix. In the presence of a matrix, cluster ions are, however, mostly suppressed. We have compared the number of analyte signals, accuracy of determination of isotope composition of the analyte and the sensitivity of FeCl3 detection between different approaches. The results obtained imply that the sensitivity of mass spectrometric analysis of FeCl3 is somewhat higher when matrices are applied than in the matrix-free approach. Among all matrices tested in this work, F20TPP seems to be the most promising for further applications as a matrix for mass spectrometry of inorganic salts.
T2  - European Journal of Mass Spectrometry
T1  - Sensitivity and accuracy of organic matrix-assisted Laser desorption and ionisation mass spectrometry of FeCl3 is higher than in in matrix-free approach
VL  - 19
IS  - 2
SP  - 77
EP  - 89
DO  - 10.1255/ejms.1217
ER  - 
@article{
author = "Radisavljevic, Maja and Kamceva, Tina and Vukićević, Iva A. and Nišavić, Marija and Milovanovic, Milan and Petković, Marijana",
year = "2013",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5605",
abstract = "We compare the quality and reliability of laser desorption and ionisation mass spectra of FeCl3 acquired without the assistance of the matrix with the spectra acquired with different organic matrix molecules. Generally, inorganic salts tend to form clusters upon laser irradiation, the signals of which can be easily distinguished from ions arising from the matrix. In the presence of a matrix, cluster ions are, however, mostly suppressed. We have compared the number of analyte signals, accuracy of determination of isotope composition of the analyte and the sensitivity of FeCl3 detection between different approaches. The results obtained imply that the sensitivity of mass spectrometric analysis of FeCl3 is somewhat higher when matrices are applied than in the matrix-free approach. Among all matrices tested in this work, F20TPP seems to be the most promising for further applications as a matrix for mass spectrometry of inorganic salts.",
journal = "European Journal of Mass Spectrometry",
title = "Sensitivity and accuracy of organic matrix-assisted Laser desorption and ionisation mass spectrometry of FeCl3 is higher than in in matrix-free approach",
volume = "19",
number = "2",
pages = "77-89",
doi = "10.1255/ejms.1217"
}
Radisavljevic, M., Kamceva, T., Vukićević, I. A., Nišavić, M., Milovanovic, M.,& Petković, M. (2013). Sensitivity and accuracy of organic matrix-assisted Laser desorption and ionisation mass spectrometry of FeCl3 is higher than in in matrix-free approach.
European Journal of Mass Spectrometry, 19(2), 77-89.
https://doi.org/10.1255/ejms.1217
Radisavljevic M, Kamceva T, Vukićević IA, Nišavić M, Milovanovic M, Petković M. Sensitivity and accuracy of organic matrix-assisted Laser desorption and ionisation mass spectrometry of FeCl3 is higher than in in matrix-free approach. European Journal of Mass Spectrometry. 2013;19(2):77-89
Radisavljevic Maja, Kamceva Tina, Vukićević Iva A., Nišavić Marija, Milovanovic Milan, Petković Marijana, "Sensitivity and accuracy of organic matrix-assisted Laser desorption and ionisation mass spectrometry of FeCl3 is higher than in in matrix-free approach" European Journal of Mass Spectrometry, 19, no. 2 (2013):77-89,
https://doi.org/10.1255/ejms.1217 .
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Interactions of Platinum and Ruthenium Coordination Complexes with Pancreatic Phospholipase A(2) and Phospholipids Investigated by MALDI TOF Mass Spectrometry

Kamceva, Tina; Radisavljevic, Maja; Vukićević, Iva A.; Arnhold, Juergen; Petković, Marijana

(2013)

TY  - JOUR
AU  - Kamceva, Tina
AU  - Radisavljevic, Maja
AU  - Vukićević, Iva A.
AU  - Arnhold, Juergen
AU  - Petković, Marijana
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5790
AB  - Phospholipase A(2) is involved in propagation of inflammatory processes and carcinogenesis through its role in phospholipid metabolism, and release of arachidonic acid and lysophospholipids. Recent findings on correlation between elevated PLA(2) activity and metastatic cancer render this enzyme an attractive target for cancer therapy. On the other hand, due to a broad range of oxidation states under physiological conditions and a high affinity for protein binding, platinum and ruthenium coordination complexes are promising candidates for PLA(2) inhibitors. In this article, we discuss the interactions of Pt and Ru coordination complexes with PLA(2) and phospholipids, as well as the application of MALDI-TOF mass spectrometry for screening PLA(2) inhibitors. Owing to the ability of this technique to simultaneously detect and monitor changes in substrate and product concentrations, the inhibitor mechanisms of both Pt and Ru complexes with various ligands were determined.
T2  - Chemistry and Biodiversity
T1  - Interactions of Platinum and Ruthenium Coordination Complexes with Pancreatic Phospholipase A(2) and Phospholipids Investigated by MALDI TOF Mass Spectrometry
VL  - 10
IS  - 11
SP  - 1972
EP  - 1986
DO  - 10.1002/cbdv.201300141
ER  - 
@article{
author = "Kamceva, Tina and Radisavljevic, Maja and Vukićević, Iva A. and Arnhold, Juergen and Petković, Marijana",
year = "2013",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5790",
abstract = "Phospholipase A(2) is involved in propagation of inflammatory processes and carcinogenesis through its role in phospholipid metabolism, and release of arachidonic acid and lysophospholipids. Recent findings on correlation between elevated PLA(2) activity and metastatic cancer render this enzyme an attractive target for cancer therapy. On the other hand, due to a broad range of oxidation states under physiological conditions and a high affinity for protein binding, platinum and ruthenium coordination complexes are promising candidates for PLA(2) inhibitors. In this article, we discuss the interactions of Pt and Ru coordination complexes with PLA(2) and phospholipids, as well as the application of MALDI-TOF mass spectrometry for screening PLA(2) inhibitors. Owing to the ability of this technique to simultaneously detect and monitor changes in substrate and product concentrations, the inhibitor mechanisms of both Pt and Ru complexes with various ligands were determined.",
journal = "Chemistry and Biodiversity",
title = "Interactions of Platinum and Ruthenium Coordination Complexes with Pancreatic Phospholipase A(2) and Phospholipids Investigated by MALDI TOF Mass Spectrometry",
volume = "10",
number = "11",
pages = "1972-1986",
doi = "10.1002/cbdv.201300141"
}
Kamceva, T., Radisavljevic, M., Vukićević, I. A., Arnhold, J.,& Petković, M. (2013). Interactions of Platinum and Ruthenium Coordination Complexes with Pancreatic Phospholipase A(2) and Phospholipids Investigated by MALDI TOF Mass Spectrometry.
Chemistry and Biodiversity, 10(11), 1972-1986.
https://doi.org/10.1002/cbdv.201300141
Kamceva T, Radisavljevic M, Vukićević IA, Arnhold J, Petković M. Interactions of Platinum and Ruthenium Coordination Complexes with Pancreatic Phospholipase A(2) and Phospholipids Investigated by MALDI TOF Mass Spectrometry. Chemistry and Biodiversity. 2013;10(11):1972-1986
Kamceva Tina, Radisavljevic Maja, Vukićević Iva A., Arnhold Juergen, Petković Marijana, "Interactions of Platinum and Ruthenium Coordination Complexes with Pancreatic Phospholipase A(2) and Phospholipids Investigated by MALDI TOF Mass Spectrometry" Chemistry and Biodiversity, 10, no. 11 (2013):1972-1986,
https://doi.org/10.1002/cbdv.201300141 .
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Colloidal TiO2 nanoparticles as substrates for M(S)ALDI mass spectrometry of transition metal complexes

Radisavljevic, Maja; Kamceva, Tina; Vukićević, Iva A.; Radoičić, Marija B.; Šaponjić, Zoran; Petković, Marijana

(2012)

TY  - JOUR
AU  - Radisavljevic, Maja
AU  - Kamceva, Tina
AU  - Vukićević, Iva A.
AU  - Radoičić, Marija B.
AU  - Šaponjić, Zoran
AU  - Petković, Marijana
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4972
AB  - RATIONALE Nanoparticles as substrates for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) have advantages over organic matrices, since they enable acquisition of spectra in the low-mass range. It has been previously shown that TiO2 nanoparticles can be used as substrate for MALDI-TOF MS analysis of phospholipids and for other types of molecules, but none of them was applied to the analysis of transition metal complexes. METHODS The MALDI-TOF mass spectra of potential anti-tumor drugs [AuCl2(bipy)]Cl, [PtCl4(bipy)], and [RuCl2(bipy)2]Cl acquired with organic matrices have been compared with spectra acquired with colloidal titanium dioxide nanoparticles. Colloidal TiO2 nanoparticles (NPs) with average diameter of 5 nm were synthesized and characterized by microscopy. For some experiments, the TiO2 NPs were treated at 60 degrees C. Suspensions of matrix and the analyte were premixed, applied to the MALDI target and left at room temperature. Mass spectra were acquired with a 50-Hz pulsed nitrogen laser emitting at a wavelength of 337 nm. RESULTS The MALDI spectra of transition metal complexes acquired with TiO2 NPs exhibited somewhat lower sensitivity than those with organic matrices; on the other hand, they are characterized by significantly lower number of signals arising from the tested complexes than the organic matrices. Whereas adducts between organic matrices and the analytes were detectable in the spectra, this was not the case for the TiO2-assisted mass spectra. CONCLUSIONS We have shown that colloidal TiO2 NPs can be used as substrates for MALDI-TOF MS of transition metal complexes. Although the sensitivity of this approach in comparison with the use of organic matrices might still be a problem, the potential of the applications of NPs for the mass spectrometric characterization of transition metal complexes is clearly demonstrated. Copyright (C) 2012 John Wiley and Sons, Ltd.
T2  - Rapid Communications in Mass Spectrometry
T1  - Colloidal TiO2 nanoparticles as substrates for M(S)ALDI mass spectrometry of transition metal complexes
VL  - 26
IS  - 17
SP  - 2041
EP  - 2050
DO  - 10.1002/rcm.6320
ER  - 
@article{
author = "Radisavljevic, Maja and Kamceva, Tina and Vukićević, Iva A. and Radoičić, Marija B. and Šaponjić, Zoran and Petković, Marijana",
year = "2012",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4972",
abstract = "RATIONALE Nanoparticles as substrates for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) have advantages over organic matrices, since they enable acquisition of spectra in the low-mass range. It has been previously shown that TiO2 nanoparticles can be used as substrate for MALDI-TOF MS analysis of phospholipids and for other types of molecules, but none of them was applied to the analysis of transition metal complexes. METHODS The MALDI-TOF mass spectra of potential anti-tumor drugs [AuCl2(bipy)]Cl, [PtCl4(bipy)], and [RuCl2(bipy)2]Cl acquired with organic matrices have been compared with spectra acquired with colloidal titanium dioxide nanoparticles. Colloidal TiO2 nanoparticles (NPs) with average diameter of 5 nm were synthesized and characterized by microscopy. For some experiments, the TiO2 NPs were treated at 60 degrees C. Suspensions of matrix and the analyte were premixed, applied to the MALDI target and left at room temperature. Mass spectra were acquired with a 50-Hz pulsed nitrogen laser emitting at a wavelength of 337 nm. RESULTS The MALDI spectra of transition metal complexes acquired with TiO2 NPs exhibited somewhat lower sensitivity than those with organic matrices; on the other hand, they are characterized by significantly lower number of signals arising from the tested complexes than the organic matrices. Whereas adducts between organic matrices and the analytes were detectable in the spectra, this was not the case for the TiO2-assisted mass spectra. CONCLUSIONS We have shown that colloidal TiO2 NPs can be used as substrates for MALDI-TOF MS of transition metal complexes. Although the sensitivity of this approach in comparison with the use of organic matrices might still be a problem, the potential of the applications of NPs for the mass spectrometric characterization of transition metal complexes is clearly demonstrated. Copyright (C) 2012 John Wiley and Sons, Ltd.",
journal = "Rapid Communications in Mass Spectrometry",
title = "Colloidal TiO2 nanoparticles as substrates for M(S)ALDI mass spectrometry of transition metal complexes",
volume = "26",
number = "17",
pages = "2041-2050",
doi = "10.1002/rcm.6320"
}
Radisavljevic, M., Kamceva, T., Vukićević, I. A., Radoičić, M. B., Šaponjić, Z.,& Petković, M. (2012). Colloidal TiO2 nanoparticles as substrates for M(S)ALDI mass spectrometry of transition metal complexes.
Rapid Communications in Mass Spectrometry, 26(17), 2041-2050.
https://doi.org/10.1002/rcm.6320
Radisavljevic M, Kamceva T, Vukićević IA, Radoičić MB, Šaponjić Z, Petković M. Colloidal TiO2 nanoparticles as substrates for M(S)ALDI mass spectrometry of transition metal complexes. Rapid Communications in Mass Spectrometry. 2012;26(17):2041-2050
Radisavljevic Maja, Kamceva Tina, Vukićević Iva A., Radoičić Marija B., Šaponjić Zoran, Petković Marijana, "Colloidal TiO2 nanoparticles as substrates for M(S)ALDI mass spectrometry of transition metal complexes" Rapid Communications in Mass Spectrometry, 26, no. 17 (2012):2041-2050,
https://doi.org/10.1002/rcm.6320 .
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Estradiol enhances effects of fructose rich diet on cardiac fatty acid transporter CD36 and triglycerides accumulation

Korićanac, Goran; Tepavčević, Snežana; Romić, Snježana Đ.; Živković, Maja; Stojiljković, Mojca D.; Milosavljević, Tijana; Stanković, Aleksandra; Petković, Marijana; Kamceva, Tina; Žakula, Zorica

(2012)

TY  - JOUR
AU  - Korićanac, Goran
AU  - Tepavčević, Snežana
AU  - Romić, Snježana Đ.
AU  - Živković, Maja
AU  - Stojiljković, Mojca D.
AU  - Milosavljević, Tijana
AU  - Stanković, Aleksandra
AU  - Petković, Marijana
AU  - Kamceva, Tina
AU  - Žakula, Zorica
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5104
AB  - Fructose rich diet increases hepatic triglycerides production and has deleterious cardiac effects. Estrogens are involved in regulation of lipid metabolism as well, but their effects are cardio beneficial. In order to study effects of fructose rich diet on the main heart fatty acid transporter CD36 and the role of estrogens, we subjected ovariectomized female rats to the standard diet or fructose rich diet, with or without estradiol (E2) replacement. The following parameters were analyzed: feeding behavior, visceral adipose tissue mass, plasma lipids, cardiac CD36 expression, localization and insulin regulation, as well as the profile of cardiac lipids. Results show that fructose rich diet significantly increased plasma triglycerides and decreased plasma free fatty acid (FFA) concentration, while E2 additionally emphasized FFA decrease. The fructose diet increased cardiac plasma membrane content of CD36 in the basal and insulin-stimulated states, and decreased its low density microsomes content. The E2 in fructose-fed rats raised the total cardiac protein content of CD36, its presence in plasma membranes and low density microsomes, and cardiac deposition of triglycerides, as well. Although E2 counteracts fructose in some aspects of lipid metabolism, and separately they have opposite cardiac effects, in combination with fructose rich diet, E2 additionally enhances CD36 presence in plasma membranes of cardiac cells and triglycerides accumulation, which paradoxically might promote deleterious effects of fructose diet on cardiac lipid metabolism. Taken together, the results presented in this work are of high importance for clinical administration of estrogens in females with a history of type 2 diabetes. (C) 2012 Elsevier B.V. All rights reserved.
T2  - European Journal of Pharmacology
T1  - Estradiol enhances effects of fructose rich diet on cardiac fatty acid transporter CD36 and triglycerides accumulation
VL  - 694
IS  - 1-3
SP  - 127
EP  - 134
DO  - 10.1016/j.ejphar.2012.08.007
ER  - 
@article{
author = "Korićanac, Goran and Tepavčević, Snežana and Romić, Snježana Đ. and Živković, Maja and Stojiljković, Mojca D. and Milosavljević, Tijana and Stanković, Aleksandra and Petković, Marijana and Kamceva, Tina and Žakula, Zorica",
year = "2012",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5104",
abstract = "Fructose rich diet increases hepatic triglycerides production and has deleterious cardiac effects. Estrogens are involved in regulation of lipid metabolism as well, but their effects are cardio beneficial. In order to study effects of fructose rich diet on the main heart fatty acid transporter CD36 and the role of estrogens, we subjected ovariectomized female rats to the standard diet or fructose rich diet, with or without estradiol (E2) replacement. The following parameters were analyzed: feeding behavior, visceral adipose tissue mass, plasma lipids, cardiac CD36 expression, localization and insulin regulation, as well as the profile of cardiac lipids. Results show that fructose rich diet significantly increased plasma triglycerides and decreased plasma free fatty acid (FFA) concentration, while E2 additionally emphasized FFA decrease. The fructose diet increased cardiac plasma membrane content of CD36 in the basal and insulin-stimulated states, and decreased its low density microsomes content. The E2 in fructose-fed rats raised the total cardiac protein content of CD36, its presence in plasma membranes and low density microsomes, and cardiac deposition of triglycerides, as well. Although E2 counteracts fructose in some aspects of lipid metabolism, and separately they have opposite cardiac effects, in combination with fructose rich diet, E2 additionally enhances CD36 presence in plasma membranes of cardiac cells and triglycerides accumulation, which paradoxically might promote deleterious effects of fructose diet on cardiac lipid metabolism. Taken together, the results presented in this work are of high importance for clinical administration of estrogens in females with a history of type 2 diabetes. (C) 2012 Elsevier B.V. All rights reserved.",
journal = "European Journal of Pharmacology",
title = "Estradiol enhances effects of fructose rich diet on cardiac fatty acid transporter CD36 and triglycerides accumulation",
volume = "694",
number = "1-3",
pages = "127-134",
doi = "10.1016/j.ejphar.2012.08.007"
}
Korićanac, G., Tepavčević, S., Romić, S. Đ., Živković, M., Stojiljković, M. D., Milosavljević, T., Stanković, A., Petković, M., Kamceva, T.,& Žakula, Z. (2012). Estradiol enhances effects of fructose rich diet on cardiac fatty acid transporter CD36 and triglycerides accumulation.
European Journal of Pharmacology, 694(1-3), 127-134.
https://doi.org/10.1016/j.ejphar.2012.08.007
Korićanac G, Tepavčević S, Romić SĐ, Živković M, Stojiljković MD, Milosavljević T, Stanković A, Petković M, Kamceva T, Žakula Z. Estradiol enhances effects of fructose rich diet on cardiac fatty acid transporter CD36 and triglycerides accumulation. European Journal of Pharmacology. 2012;694(1-3):127-134
Korićanac Goran, Tepavčević Snežana, Romić Snježana Đ., Živković Maja, Stojiljković Mojca D., Milosavljević Tijana, Stanković Aleksandra, Petković Marijana, Kamceva Tina, Žakula Zorica, "Estradiol enhances effects of fructose rich diet on cardiac fatty acid transporter CD36 and triglycerides accumulation" European Journal of Pharmacology, 694, no. 1-3 (2012):127-134,
https://doi.org/10.1016/j.ejphar.2012.08.007 .
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Laser desorption and ionization time-of-flight versus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of Pt(II) and Ru(III) metal complexes

Damnjanovic, Bojana; Kamceva, Tina; Petrovic, Biljana; Bugarcic, Zivadin D.; Petković, Marijana

(2011)

TY  - JOUR
AU  - Damnjanovic, Bojana
AU  - Kamceva, Tina
AU  - Petrovic, Biljana
AU  - Bugarcic, Zivadin D.
AU  - Petković, Marijana
PY  - 2011
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4237
AB  - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently established as a powerful, soft ionization technique for the analysis of both transition metal complexes, which are used as metallo-drugs in the therapy of various types of tumors, and biomolecules. Since some metal complexes absorb light in the UV range, it should be possible to analyse them without additional matrices, i.e. using LDI-TOF MS. In this study, the matrix-free approach was tested for the analysis of [PtCl(2)(dach)] (dichloride(1,2-diamincyclohexane) platinum(II)), [RuCl(2)(en)(2)]Cl (dichloridobis(ethylenediamine) ruthenium(III) chloride) and [RuCl(2)(bipy)(2)]Cl (bis(bipyridine)dichloridoruthenium(III) chloride) and the detection limit for these compounds was determined. In summary, the LDI-TOF mass spectra of [PtCl(2)(dach)] and [RuCl(2)(en)(2)]Cl are rather simple, whereas in the presence of 2,5-DHB as a matrix, additional peaks are generated. On the other hand, the standard MALDI-TOF mass spectrum of [RuCl(2)(bipy)(2)]Cl exhibits only one peak arising from the complex, in contrast to six peaks detectable in the LDI-TOF mass spectrum. The detection limit in the MALDI-TOF MS analysis of [PtCl(2)(dach)] and [RuCl(2)(bipy)(2)]Cl complexes was lower than that determined in LDI-TOF MS. Taking all into account, in this paper, we have demonstrated some advantages and drawbacks of the matrix-free LDI-TOF mass spectrometric analysis of transition metal complexes.
T2  - Analytical Methods
T1  - Laser desorption and ionization time-of-flight versus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of Pt(II) and Ru(III) metal complexes
VL  - 3
IS  - 2
SP  - 400
EP  - 407
DO  - 10.1039/c0ay00517g
ER  - 
@article{
author = "Damnjanovic, Bojana and Kamceva, Tina and Petrovic, Biljana and Bugarcic, Zivadin D. and Petković, Marijana",
year = "2011",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4237",
abstract = "Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently established as a powerful, soft ionization technique for the analysis of both transition metal complexes, which are used as metallo-drugs in the therapy of various types of tumors, and biomolecules. Since some metal complexes absorb light in the UV range, it should be possible to analyse them without additional matrices, i.e. using LDI-TOF MS. In this study, the matrix-free approach was tested for the analysis of [PtCl(2)(dach)] (dichloride(1,2-diamincyclohexane) platinum(II)), [RuCl(2)(en)(2)]Cl (dichloridobis(ethylenediamine) ruthenium(III) chloride) and [RuCl(2)(bipy)(2)]Cl (bis(bipyridine)dichloridoruthenium(III) chloride) and the detection limit for these compounds was determined. In summary, the LDI-TOF mass spectra of [PtCl(2)(dach)] and [RuCl(2)(en)(2)]Cl are rather simple, whereas in the presence of 2,5-DHB as a matrix, additional peaks are generated. On the other hand, the standard MALDI-TOF mass spectrum of [RuCl(2)(bipy)(2)]Cl exhibits only one peak arising from the complex, in contrast to six peaks detectable in the LDI-TOF mass spectrum. The detection limit in the MALDI-TOF MS analysis of [PtCl(2)(dach)] and [RuCl(2)(bipy)(2)]Cl complexes was lower than that determined in LDI-TOF MS. Taking all into account, in this paper, we have demonstrated some advantages and drawbacks of the matrix-free LDI-TOF mass spectrometric analysis of transition metal complexes.",
journal = "Analytical Methods",
title = "Laser desorption and ionization time-of-flight versus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of Pt(II) and Ru(III) metal complexes",
volume = "3",
number = "2",
pages = "400-407",
doi = "10.1039/c0ay00517g"
}
Damnjanovic, B., Kamceva, T., Petrovic, B., Bugarcic, Z. D.,& Petković, M. (2011). Laser desorption and ionization time-of-flight versus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of Pt(II) and Ru(III) metal complexes.
Analytical Methods, 3(2), 400-407.
https://doi.org/10.1039/c0ay00517g
Damnjanovic B, Kamceva T, Petrovic B, Bugarcic ZD, Petković M. Laser desorption and ionization time-of-flight versus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of Pt(II) and Ru(III) metal complexes. Analytical Methods. 2011;3(2):400-407
Damnjanovic Bojana, Kamceva Tina, Petrovic Biljana, Bugarcic Zivadin D., Petković Marijana, "Laser desorption and ionization time-of-flight versus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of Pt(II) and Ru(III) metal complexes" Analytical Methods, 3, no. 2 (2011):400-407,
https://doi.org/10.1039/c0ay00517g .
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FAB, ESI and MALDI Mass Spectrometric methods in the study of metallo-drugs and their biomolecular interactions

Petković, Marijana; Kamceva, Tina

(2011)

TY  - JOUR
AU  - Petković, Marijana
AU  - Kamceva, Tina
PY  - 2011
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4336
AB  - This review surveys the soft ionisation mass spectrometric methods that are most commonly used for the investigation of the mechanism of interaction between metallo-drugs and biomolecules. In the first part of the review, an overview of the applications of transition metal complexes in the therapy of various diseases (arthritis, cancer, diabetes) is given, whereas the second part focuses on the obtained results dealing with various aspects of the interaction between metal complexes and different types of biomolecules. Possibilities and limitations of each mass spectrometric method-namely, fast atom bombardment (FAB), electrospray ionisation (ESI) and matrix-assisted laser desorption and ionisation (MALDI)-are discussed in the third part of this review along with the examples of their application for the analysis of metal complexes, as well as of the products of their interaction with biomolecules.
T2  - Metallomics
T1  - FAB, ESI and MALDI Mass Spectrometric methods in the study of metallo-drugs and their biomolecular interactions
VL  - 3
IS  - 6
SP  - 550
EP  - 565
DO  - 10.1039/c0mt00096e
ER  - 
@article{
author = "Petković, Marijana and Kamceva, Tina",
year = "2011",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4336",
abstract = "This review surveys the soft ionisation mass spectrometric methods that are most commonly used for the investigation of the mechanism of interaction between metallo-drugs and biomolecules. In the first part of the review, an overview of the applications of transition metal complexes in the therapy of various diseases (arthritis, cancer, diabetes) is given, whereas the second part focuses on the obtained results dealing with various aspects of the interaction between metal complexes and different types of biomolecules. Possibilities and limitations of each mass spectrometric method-namely, fast atom bombardment (FAB), electrospray ionisation (ESI) and matrix-assisted laser desorption and ionisation (MALDI)-are discussed in the third part of this review along with the examples of their application for the analysis of metal complexes, as well as of the products of their interaction with biomolecules.",
journal = "Metallomics",
title = "FAB, ESI and MALDI Mass Spectrometric methods in the study of metallo-drugs and their biomolecular interactions",
volume = "3",
number = "6",
pages = "550-565",
doi = "10.1039/c0mt00096e"
}
Petković, M.,& Kamceva, T. (2011). FAB, ESI and MALDI Mass Spectrometric methods in the study of metallo-drugs and their biomolecular interactions.
Metallomics, 3(6), 550-565.
https://doi.org/10.1039/c0mt00096e
Petković M, Kamceva T. FAB, ESI and MALDI Mass Spectrometric methods in the study of metallo-drugs and their biomolecular interactions. Metallomics. 2011;3(6):550-565
Petković Marijana, Kamceva Tina, "FAB, ESI and MALDI Mass Spectrometric methods in the study of metallo-drugs and their biomolecular interactions" Metallomics, 3, no. 6 (2011):550-565,
https://doi.org/10.1039/c0mt00096e .
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Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2)

Kamceva, Tina; Flemmig, Joerg; Damnjanovic, Bojana; Arnhold, Juergen; Mijatovic, Aleksandar; Petković, Marijana

(2011)

TY  - JOUR
AU  - Kamceva, Tina
AU  - Flemmig, Joerg
AU  - Damnjanovic, Bojana
AU  - Arnhold, Juergen
AU  - Mijatovic, Aleksandar
AU  - Petković, Marijana
PY  - 2011
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4514
AB  - Pancreatic phospholipase A(2) (PLA(2)) plays an important role in cellular homeostasis as well as in the process of carcinogenesis. Effects of metallo-drugs used as chemotherapeutics on the activity of this enzyme are unknown. In this work, the interaction between porcine pancreatic PLA(2) and two selected transition metal complexes-tetrachloro(bipyridine) platinum(IV) ([PtCl4(bipy)]) and dichloro (bipyridine) ruthenium(III)chloride ([RuCl2(bipy)(2)]Cl)-was studied. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and fluorescence spectroscopy have been used to analyse the enzyme activity in the absence and presence of metal complexes and to verify potential binding of these drugs to the enzyme. The tested metal complexes decreased the activity of phospholipase A(2) in an uncompetitive inhibition mode. A binding of the ruthenium complex near the active site of the enzyme could be evidenced and possible modes of interaction are discussed.
T2  - Metallomics
T1  - Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2)
VL  - 3
IS  - 10
SP  - 1056
EP  - 1063
DO  - 10.1039/c1mt00088h
ER  - 
@article{
author = "Kamceva, Tina and Flemmig, Joerg and Damnjanovic, Bojana and Arnhold, Juergen and Mijatovic, Aleksandar and Petković, Marijana",
year = "2011",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4514",
abstract = "Pancreatic phospholipase A(2) (PLA(2)) plays an important role in cellular homeostasis as well as in the process of carcinogenesis. Effects of metallo-drugs used as chemotherapeutics on the activity of this enzyme are unknown. In this work, the interaction between porcine pancreatic PLA(2) and two selected transition metal complexes-tetrachloro(bipyridine) platinum(IV) ([PtCl4(bipy)]) and dichloro (bipyridine) ruthenium(III)chloride ([RuCl2(bipy)(2)]Cl)-was studied. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and fluorescence spectroscopy have been used to analyse the enzyme activity in the absence and presence of metal complexes and to verify potential binding of these drugs to the enzyme. The tested metal complexes decreased the activity of phospholipase A(2) in an uncompetitive inhibition mode. A binding of the ruthenium complex near the active site of the enzyme could be evidenced and possible modes of interaction are discussed.",
journal = "Metallomics",
title = "Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2)",
volume = "3",
number = "10",
pages = "1056-1063",
doi = "10.1039/c1mt00088h"
}
Kamceva, T., Flemmig, J., Damnjanovic, B., Arnhold, J., Mijatovic, A.,& Petković, M. (2011). Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2).
Metallomics, 3(10), 1056-1063.
https://doi.org/10.1039/c1mt00088h
Kamceva T, Flemmig J, Damnjanovic B, Arnhold J, Mijatovic A, Petković M. Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2). Metallomics. 2011;3(10):1056-1063
Kamceva Tina, Flemmig Joerg, Damnjanovic Bojana, Arnhold Juergen, Mijatovic Aleksandar, Petković Marijana, "Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2)" Metallomics, 3, no. 10 (2011):1056-1063,
https://doi.org/10.1039/c1mt00088h .
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