TY  - JOUR
AU  - Petronijević, Jelena
AU  - Joksimović, Nenad
AU  - Milović, Emilija
AU  - Đorđić Crnogorac, Marija
AU  - Petrović, Nina
AU  - Stanojković, Tatjana P.
AU  - Milivojević, Dušan
AU  - Janković, Nenad
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9933
AB  - In order to discover new therapeutically active agents a series of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones were synthesized. All complexes were characterized by IR and EPR spectroscopic techniques and examined for their cytotoxic effect on human cancer cell lines HeLa, LS174, A549 and normal fibroblasts (MRC-5). For further examination of the cytotoxic mechanisms of novel complexes, three of them were chosen for analysing their effects on the distribution of HeLa cells in the cell cycle phases. The results of the flow cytometry analysis suggest that tested complexes lead to time-dependent accumulation of the cells in S and G2/M phases. The strongest accumulation effect showed complex 2d after 48 h of incubation. Competitive experiments with ethidium bromide (EB) indicated that tested compound 2d have affinity to displace EB from the EB-DNA complex through intercalation. Also, the binding parameters values for 2d-BSA complex showed that a reversible 2d-BSA complex is formed and ligand 2d can be stored and carried by BSA.
T2  - Chemico-Biological Interactions
T1  - Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones
VL  - 348
SP  - 109647
DO  - 10.1016/j.cbi.2021.109647
ER  - 

@article{
author = "Petronijević, Jelena and Joksimović, Nenad and Milović, Emilija and Đorđić Crnogorac, Marija and Petrović, Nina and Stanojković, Tatjana P. and Milivojević, Dušan and Janković, Nenad",
year = "2021",
abstract = "In order to discover new therapeutically active agents a series of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones were synthesized. All complexes were characterized by IR and EPR spectroscopic techniques and examined for their cytotoxic effect on human cancer cell lines HeLa, LS174, A549 and normal fibroblasts (MRC-5). For further examination of the cytotoxic mechanisms of novel complexes, three of them were chosen for analysing their effects on the distribution of HeLa cells in the cell cycle phases. The results of the flow cytometry analysis suggest that tested complexes lead to time-dependent accumulation of the cells in S and G2/M phases. The strongest accumulation effect showed complex 2d after 48 h of incubation. Competitive experiments with ethidium bromide (EB) indicated that tested compound 2d have affinity to displace EB from the EB-DNA complex through intercalation. Also, the binding parameters values for 2d-BSA complex showed that a reversible 2d-BSA complex is formed and ligand 2d can be stored and carried by BSA.",
journal = "Chemico-Biological Interactions",
title = "Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones",
volume = "348",
pages = "109647",
doi = "10.1016/j.cbi.2021.109647"
}

Petronijević, J., Joksimović, N., Milović, E., Đorđić Crnogorac, M., Petrović, N., Stanojković, T. P., Milivojević, D.,& Janković, N.. (2021). Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones. in Chemico-Biological Interactions, 348, 109647.
https://doi.org/10.1016/j.cbi.2021.109647

Petronijević J, Joksimović N, Milović E, Đorđić Crnogorac M, Petrović N, Stanojković TP, Milivojević D, Janković N. Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones. in Chemico-Biological Interactions. 2021;348:109647.
doi:10.1016/j.cbi.2021.109647 .

Petronijević, Jelena, Joksimović, Nenad, Milović, Emilija, Đorđić Crnogorac, Marija, Petrović, Nina, Stanojković, Tatjana P., Milivojević, Dušan, Janković, Nenad, "Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones" in Chemico-Biological Interactions, 348 (2021):109647,
https://doi.org/10.1016/j.cbi.2021.109647 . .