TY  - JOUR
AU  - Srdić-Rajić, Tatjana
AU  - Jurišić, Vladimir
AU  - Andrejevic, Sladjana
AU  - Bonaci-Nikolic, Branka
AU  - Bowker, Timothy
AU  - Concas, Daniela
AU  - Metlaš, Radmila
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4657
AB  - The production of autoantibodies against a vast array of self antigens, most notably double stranded (ds) DNA, characterized systemic lupus erythematosus (SLE). The purpose of this work is to study specific Ig fractions isolated from normal human serum (NHS) and their effect on the binding of anti-double-stranded deoxyribonucleic acid (dsDNA) antibodies (Abs) to dsDNA. A fraction named immunoglobulin G (IgG)-reactive IgG was purified from total NHS IgG by absorption onto (CNBr)-activated Sepharose 4B linked to intact IgG molecules (IgG-Sepharose column). IgG-reactive IgG was co-incubated with systemic lupus erythematosus (SLE) patients serum and binding of the anti-dsDNA Abs to dsDNA was measured by enzyme-linked immunosorbent assay (ELISA). Co-incubation of SLE patients serum with IgG-reactive IgG resulted in a dose-dependent reduction in binding of anti-dsDNA Abs to dsDNA. A reduction greater than 70% was observed at a concentration of 300 mu g of IgG-reactive IgG per mL of a 400-fold diluted SLE patients serum whereas total NHS IgG, at the same concentration, resulted in a 10% reduction in binding. The purification process used to isolate IgG-reactive IgG was based on interactions between intact Ig rather than on interactions between F(ab)(2) portions. IgG(2) is the predominant immunoglobulin (Ig) subclass in IgG-reactive IgG. Thus, IgG(2) might have an important role in the connectivity characteristics of NHS IgG. The capacity of IgG-reactive IgG to inhibit anti-DNA Ab binding to dsDNA may have potential application in the treatment of SLE. This targeted biological approach may provide an alternative strategy to immunosuppressants. (C) 2011 Elsevier GmbH. All rights reserved.
T2  - Immunobiology
T1  - Naturally occurring V region connected antibodies inhibit anti-dsDNA antibody reactivity with dsDNA
VL  - 217
IS  - 1
SP  - 111
EP  - 117
DO  - 10.1016/j.imbio.2011.07.026
ER  - 

@article{
author = "Srdić-Rajić, Tatjana and Jurišić, Vladimir and Andrejevic, Sladjana and Bonaci-Nikolic, Branka and Bowker, Timothy and Concas, Daniela and Metlaš, Radmila",
year = "2012",
abstract = "The production of autoantibodies against a vast array of self antigens, most notably double stranded (ds) DNA, characterized systemic lupus erythematosus (SLE). The purpose of this work is to study specific Ig fractions isolated from normal human serum (NHS) and their effect on the binding of anti-double-stranded deoxyribonucleic acid (dsDNA) antibodies (Abs) to dsDNA. A fraction named immunoglobulin G (IgG)-reactive IgG was purified from total NHS IgG by absorption onto (CNBr)-activated Sepharose 4B linked to intact IgG molecules (IgG-Sepharose column). IgG-reactive IgG was co-incubated with systemic lupus erythematosus (SLE) patients serum and binding of the anti-dsDNA Abs to dsDNA was measured by enzyme-linked immunosorbent assay (ELISA). Co-incubation of SLE patients serum with IgG-reactive IgG resulted in a dose-dependent reduction in binding of anti-dsDNA Abs to dsDNA. A reduction greater than 70% was observed at a concentration of 300 mu g of IgG-reactive IgG per mL of a 400-fold diluted SLE patients serum whereas total NHS IgG, at the same concentration, resulted in a 10% reduction in binding. The purification process used to isolate IgG-reactive IgG was based on interactions between intact Ig rather than on interactions between F(ab)(2) portions. IgG(2) is the predominant immunoglobulin (Ig) subclass in IgG-reactive IgG. Thus, IgG(2) might have an important role in the connectivity characteristics of NHS IgG. The capacity of IgG-reactive IgG to inhibit anti-DNA Ab binding to dsDNA may have potential application in the treatment of SLE. This targeted biological approach may provide an alternative strategy to immunosuppressants. (C) 2011 Elsevier GmbH. All rights reserved.",
journal = "Immunobiology",
title = "Naturally occurring V region connected antibodies inhibit anti-dsDNA antibody reactivity with dsDNA",
volume = "217",
number = "1",
pages = "111-117",
doi = "10.1016/j.imbio.2011.07.026"
}

Srdić-Rajić, T., Jurišić, V., Andrejevic, S., Bonaci-Nikolic, B., Bowker, T., Concas, D.,& Metlaš, R.. (2012). Naturally occurring V region connected antibodies inhibit anti-dsDNA antibody reactivity with dsDNA. in Immunobiology, 217(1), 111-117.
https://doi.org/10.1016/j.imbio.2011.07.026

Srdić-Rajić T, Jurišić V, Andrejevic S, Bonaci-Nikolic B, Bowker T, Concas D, Metlaš R. Naturally occurring V region connected antibodies inhibit anti-dsDNA antibody reactivity with dsDNA. in Immunobiology. 2012;217(1):111-117.
doi:10.1016/j.imbio.2011.07.026 .

Srdić-Rajić, Tatjana, Jurišić, Vladimir, Andrejevic, Sladjana, Bonaci-Nikolic, Branka, Bowker, Timothy, Concas, Daniela, Metlaš, Radmila, "Naturally occurring V region connected antibodies inhibit anti-dsDNA antibody reactivity with dsDNA" in Immunobiology, 217, no. 1 (2012):111-117,
https://doi.org/10.1016/j.imbio.2011.07.026 . .