TY  - JOUR
AU  - Krajnović, Milena M.
AU  - Jovanovic, Maja Perunicic
AU  - Mihaljevic, Biljana
AU  - Andelic, Bosko
AU  - Tarabar, Olivera
AU  - Knežević-Ušaj, Slavica
AU  - Krtolica-Žikić, Koviljka
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/192
AB  - In this study, methylation-specific polymerase chain reaction was used to investigate the potential prognostic significance of the methylation status of p15, p16, MGMT, and DAPK genes in 51 specimens of diffuse large B-cell lymphoma (DLBCL). Hypermethylation of p15 gene was significantly more prevalent in patients without relapse (p = 0.001) and there was a trend toward more frequent presence of p15 methylation in patients without death outcome within 5-year follow-up period (p = 0.086) Also, there was a trend toward accumulation of p15 methylation with favorable clinicopathological parameters including: age 60 years (p = 0.091), normal levels of lactate dehydrogenase (p = 0.090), Eastern Cooperative Oncology Group performance status LT 2 (p = 0.095), and low/intermediate low International Prognostic Index (p = 0.076). In the female group and group of the patients without bulky tumor mass, treated with chemotherapeutic regimens including rituximab, methylation of p15 was significantly related to longer overall survival (p = 0.036 and 0.027, respectively). Our results suggest that promoter methylation of p15 gene could have prognostic value in DLBCL patients treated with rituximab when used in combination with gender and tumor size.
T2  - Clinical and Translational Science / CTS
T1  - Hypermethylation of p15 Gene in Diffuse - Large B-Cell Lymphoma: Association with Less Aggressiveness of the Disease
VL  - 7
IS  - 5
SP  - 384
EP  - 390
DO  - 10.1111/cts.12162
ER  - 

@article{
author = "Krajnović, Milena M. and Jovanovic, Maja Perunicic and Mihaljevic, Biljana and Andelic, Bosko and Tarabar, Olivera and Knežević-Ušaj, Slavica and Krtolica-Žikić, Koviljka",
year = "2014",
abstract = "In this study, methylation-specific polymerase chain reaction was used to investigate the potential prognostic significance of the methylation status of p15, p16, MGMT, and DAPK genes in 51 specimens of diffuse large B-cell lymphoma (DLBCL). Hypermethylation of p15 gene was significantly more prevalent in patients without relapse (p = 0.001) and there was a trend toward more frequent presence of p15 methylation in patients without death outcome within 5-year follow-up period (p = 0.086) Also, there was a trend toward accumulation of p15 methylation with favorable clinicopathological parameters including: age 60 years (p = 0.091), normal levels of lactate dehydrogenase (p = 0.090), Eastern Cooperative Oncology Group performance status LT 2 (p = 0.095), and low/intermediate low International Prognostic Index (p = 0.076). In the female group and group of the patients without bulky tumor mass, treated with chemotherapeutic regimens including rituximab, methylation of p15 was significantly related to longer overall survival (p = 0.036 and 0.027, respectively). Our results suggest that promoter methylation of p15 gene could have prognostic value in DLBCL patients treated with rituximab when used in combination with gender and tumor size.",
journal = "Clinical and Translational Science / CTS",
title = "Hypermethylation of p15 Gene in Diffuse - Large B-Cell Lymphoma: Association with Less Aggressiveness of the Disease",
volume = "7",
number = "5",
pages = "384-390",
doi = "10.1111/cts.12162"
}

Krajnović, M. M., Jovanovic, M. P., Mihaljevic, B., Andelic, B., Tarabar, O., Knežević-Ušaj, S.,& Krtolica-Žikić, K.. (2014). Hypermethylation of p15 Gene in Diffuse - Large B-Cell Lymphoma: Association with Less Aggressiveness of the Disease. in Clinical and Translational Science / CTS, 7(5), 384-390.
https://doi.org/10.1111/cts.12162

Krajnović MM, Jovanovic MP, Mihaljevic B, Andelic B, Tarabar O, Knežević-Ušaj S, Krtolica-Žikić K. Hypermethylation of p15 Gene in Diffuse - Large B-Cell Lymphoma: Association with Less Aggressiveness of the Disease. in Clinical and Translational Science / CTS. 2014;7(5):384-390.
doi:10.1111/cts.12162 .

Krajnović, Milena M., Jovanovic, Maja Perunicic, Mihaljevic, Biljana, Andelic, Bosko, Tarabar, Olivera, Knežević-Ušaj, Slavica, Krtolica-Žikić, Koviljka, "Hypermethylation of p15 Gene in Diffuse - Large B-Cell Lymphoma: Association with Less Aggressiveness of the Disease" in Clinical and Translational Science / CTS, 7, no. 5 (2014):384-390,
https://doi.org/10.1111/cts.12162 . .

TY  - JOUR
AU  - Kurtovic, Nada Kraguljac
AU  - Krajnović, Milena M.
AU  - Dimitrijević, Bogomir B.
AU  - Mihaljevic, Biljana
AU  - Gotić, Mirjana
AU  - Krtolica-Žikić, Koviljka
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4045
AB  - The methylation status of the target promoter sequences of p15(INK4B) (p15) and O-6-methylguanine-DNA methyltransferase (MGMT) genes was studied by methylation-specific PCR in 10 adult patients with de novo B-cell non-Hodgkin lymphoma (B-NHL). The aberrant hypermethylation of the p15 gene was more frequent (50%) compared to the hypermethylation of the MGMT gene (30%), and was detected in different types of B-NHL in both genes. Hypermethylation of the MGMT gene occurred exclusively in association with the hypermethylation of the p15 gene. All lymphoma patients with hypermethylation of the p15 and/or MGMT genes had a higher clinical stage of the disease (IV - V). We show the association of anemia and/or thrombocytopenia with the hypermethylation of the p15 gene, ascribing the p15 gene as a potential prognostic marker in B-NHL. Comethylation of MGMT with the p15 gene represents a novel finding and presents both genes as candidates for future studies of the hypermethylation profiles of B-NHL.
T2  - Archives of Biological Sciences
T1  - Frequency of Aberrant Promoter Methylation of P15(Ink4b) and O-6-Methylguanine-Dna Methyltransferase Genes in B-Cell Non-Hodgkin Lymphoma: a Pilot Study
VL  - 62
IS  - 2
SP  - 211
EP  - 221
DO  - 10.2298/ABS1002211K
ER  - 

@article{
author = "Kurtovic, Nada Kraguljac and Krajnović, Milena M. and Dimitrijević, Bogomir B. and Mihaljevic, Biljana and Gotić, Mirjana and Krtolica-Žikić, Koviljka",
year = "2010",
abstract = "The methylation status of the target promoter sequences of p15(INK4B) (p15) and O-6-methylguanine-DNA methyltransferase (MGMT) genes was studied by methylation-specific PCR in 10 adult patients with de novo B-cell non-Hodgkin lymphoma (B-NHL). The aberrant hypermethylation of the p15 gene was more frequent (50%) compared to the hypermethylation of the MGMT gene (30%), and was detected in different types of B-NHL in both genes. Hypermethylation of the MGMT gene occurred exclusively in association with the hypermethylation of the p15 gene. All lymphoma patients with hypermethylation of the p15 and/or MGMT genes had a higher clinical stage of the disease (IV - V). We show the association of anemia and/or thrombocytopenia with the hypermethylation of the p15 gene, ascribing the p15 gene as a potential prognostic marker in B-NHL. Comethylation of MGMT with the p15 gene represents a novel finding and presents both genes as candidates for future studies of the hypermethylation profiles of B-NHL.",
journal = "Archives of Biological Sciences",
title = "Frequency of Aberrant Promoter Methylation of P15(Ink4b) and O-6-Methylguanine-Dna Methyltransferase Genes in B-Cell Non-Hodgkin Lymphoma: a Pilot Study",
volume = "62",
number = "2",
pages = "211-221",
doi = "10.2298/ABS1002211K"
}

Kurtovic, N. K., Krajnović, M. M., Dimitrijević, B. B., Mihaljevic, B., Gotić, M.,& Krtolica-Žikić, K.. (2010). Frequency of Aberrant Promoter Methylation of P15(Ink4b) and O-6-Methylguanine-Dna Methyltransferase Genes in B-Cell Non-Hodgkin Lymphoma: a Pilot Study. in Archives of Biological Sciences, 62(2), 211-221.
https://doi.org/10.2298/ABS1002211K

Kurtovic NK, Krajnović MM, Dimitrijević BB, Mihaljevic B, Gotić M, Krtolica-Žikić K. Frequency of Aberrant Promoter Methylation of P15(Ink4b) and O-6-Methylguanine-Dna Methyltransferase Genes in B-Cell Non-Hodgkin Lymphoma: a Pilot Study. in Archives of Biological Sciences. 2010;62(2):211-221.
doi:10.2298/ABS1002211K .

Kurtovic, Nada Kraguljac, Krajnović, Milena M., Dimitrijević, Bogomir B., Mihaljevic, Biljana, Gotić, Mirjana, Krtolica-Žikić, Koviljka, "Frequency of Aberrant Promoter Methylation of P15(Ink4b) and O-6-Methylguanine-Dna Methyltransferase Genes in B-Cell Non-Hodgkin Lymphoma: a Pilot Study" in Archives of Biological Sciences, 62, no. 2 (2010):211-221,
https://doi.org/10.2298/ABS1002211K . .