TY  - JOUR
AU  - Figueroa, Fabian A.
AU  - Abdala-Díaz, Roberto T.
AU  - Pérez, Claudia
AU  - Casas-Arrojo, Virginia
AU  - Nešić, Aleksandra
AU  - Tapia, Cecilia
AU  - Durán, Carla
AU  - Valdes, Oscar
AU  - Parra, Carolina
AU  - Bravo-Arrepol, Gastón
AU  - Soto, Luis
AU  - Becerra, José
AU  - Cabrera-Barjas, Gustavo
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10388
AB  - Codium bernabei is a green alga that grows on Chilean coasts. The composition of its structural polysaccharides is still unknown. Hence, the aim of this work is to isolate and characterize the hot water extracted polysaccharide fractions. For this purpose, the water extracts were further precipitated in alcohol (TPs) and acid media (APs), respectively. Both fractions were characterized using different physicochemical techniques such as GC-MS, GPC, FTIR, TGA, and SEM. It is confirmed that the extracted fractions are mainly made of sulfated galactan unit, with a degree of sulfation of 19.3% (TPs) and 17.4% (ATs) and a protein content of 3.5% in APs and 15.6% in TPs. Other neutral sugars such as xylose, glucose, galactose, fucose, mannose, and arabinose were found in a molar ratio (0.05:0.6:1.0:0.02:0.14:0.11) for TPs and (0.05:0.31:1.0:0.03:0.1:0.13) for ATs. The molecular weight of the polysaccharide samples was lower than 20 kDa. Both polysaccharides were thermally stable (Tonset > 190 °C) and showed antioxidant activity according to the ABTS•+ and DPPH tests, where TPs fractions had higher scavenging activity (35%) compared to the APs fractions. The PT and APTTS assays were used to measure the anticoagulant activity of the polysaccharide fractions. In general, the PT activity of the TPs and APs was not different from normal plasma values. The exception was the TPs treatment at 1000 µg mL−1 concentration. The APTTS test revealed that clotting time for both polysaccharides was prolonged regarding normal values at 1000 µg mL−1. Finally, the antitumor test in colorectal carcinoma (HTC-116) cell line, breast cancer (MCF-7) and human leukemia (HL-60) cell lines showed the cytotoxic effect of TPs and APs. Those results suggest the potential biotechnological application of sulfate galactan polysaccharides isolated from a Chilean marine resource.
T2  - Marine Drugs
T1  - Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity
VL  - 20
IS  - 7
SP  - 458
DO  - 10.3390/md20070458
ER  - 

@article{
author = "Figueroa, Fabian A. and Abdala-Díaz, Roberto T. and Pérez, Claudia and Casas-Arrojo, Virginia and Nešić, Aleksandra and Tapia, Cecilia and Durán, Carla and Valdes, Oscar and Parra, Carolina and Bravo-Arrepol, Gastón and Soto, Luis and Becerra, José and Cabrera-Barjas, Gustavo",
year = "2022",
abstract = "Codium bernabei is a green alga that grows on Chilean coasts. The composition of its structural polysaccharides is still unknown. Hence, the aim of this work is to isolate and characterize the hot water extracted polysaccharide fractions. For this purpose, the water extracts were further precipitated in alcohol (TPs) and acid media (APs), respectively. Both fractions were characterized using different physicochemical techniques such as GC-MS, GPC, FTIR, TGA, and SEM. It is confirmed that the extracted fractions are mainly made of sulfated galactan unit, with a degree of sulfation of 19.3% (TPs) and 17.4% (ATs) and a protein content of 3.5% in APs and 15.6% in TPs. Other neutral sugars such as xylose, glucose, galactose, fucose, mannose, and arabinose were found in a molar ratio (0.05:0.6:1.0:0.02:0.14:0.11) for TPs and (0.05:0.31:1.0:0.03:0.1:0.13) for ATs. The molecular weight of the polysaccharide samples was lower than 20 kDa. Both polysaccharides were thermally stable (Tonset > 190 °C) and showed antioxidant activity according to the ABTS•+ and DPPH tests, where TPs fractions had higher scavenging activity (35%) compared to the APs fractions. The PT and APTTS assays were used to measure the anticoagulant activity of the polysaccharide fractions. In general, the PT activity of the TPs and APs was not different from normal plasma values. The exception was the TPs treatment at 1000 µg mL−1 concentration. The APTTS test revealed that clotting time for both polysaccharides was prolonged regarding normal values at 1000 µg mL−1. Finally, the antitumor test in colorectal carcinoma (HTC-116) cell line, breast cancer (MCF-7) and human leukemia (HL-60) cell lines showed the cytotoxic effect of TPs and APs. Those results suggest the potential biotechnological application of sulfate galactan polysaccharides isolated from a Chilean marine resource.",
journal = "Marine Drugs",
title = "Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity",
volume = "20",
number = "7",
pages = "458",
doi = "10.3390/md20070458"
}

Figueroa, F. A., Abdala-Díaz, R. T., Pérez, C., Casas-Arrojo, V., Nešić, A., Tapia, C., Durán, C., Valdes, O., Parra, C., Bravo-Arrepol, G., Soto, L., Becerra, J.,& Cabrera-Barjas, G.. (2022). Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity. in Marine Drugs, 20(7), 458.
https://doi.org/10.3390/md20070458

Figueroa FA, Abdala-Díaz RT, Pérez C, Casas-Arrojo V, Nešić A, Tapia C, Durán C, Valdes O, Parra C, Bravo-Arrepol G, Soto L, Becerra J, Cabrera-Barjas G. Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity. in Marine Drugs. 2022;20(7):458.
doi:10.3390/md20070458 .

Figueroa, Fabian A., Abdala-Díaz, Roberto T., Pérez, Claudia, Casas-Arrojo, Virginia, Nešić, Aleksandra, Tapia, Cecilia, Durán, Carla, Valdes, Oscar, Parra, Carolina, Bravo-Arrepol, Gastón, Soto, Luis, Becerra, José, Cabrera-Barjas, Gustavo, "Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity" in Marine Drugs, 20, no. 7 (2022):458,
https://doi.org/10.3390/md20070458 . .

TY  - JOUR
AU  - Cabrera-Barjas, Gustavo
AU  - Nešić, Aleksandra
AU  - Bravo-Arrepol, Gaston
AU  - Rodríguez-Llamazares, Saddys
AU  - Valdés, Oscar
AU  - Banerjee, Aparna
AU  - Castaño, Johanna
AU  - Delattre, Cédric
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10100
AB  - This study investigated the biocomposite pectin films enriched with murta (Ugni molinae T.) seed polyphenolic extract and reinforced by chitin nanofiber. The structural, morphological, mechanical, barrier, colorimetric, and antioxidant activity of films were evaluated. The obtained data clearly demonstrated that the addition of murta seed extract and the high load of chitin nanofibers (50%) provided more cohesive and dense morphology of films and improved the mechanical resistance and water vapor barrier in comparison to the control pectin film. The antioxidant activity ranged between 71% and 86%, depending on the film formulation and concentration of chitin nanofibers. The presented results highlight the potential use of chitin nanofibers and murta seed extract in the pectin matrix to be applied in functional food coatings and packaging, as a sustainable solution.
T2  - Molecules
T1  - Bioactive Pectin-Murta (Ugni molinae T.) Seed Extract Films Reinforced with Chitin Fibers
VL  - 26
IS  - 24
SP  - 7477
DO  - 10.3390/molecules26247477
ER  - 

@article{
author = "Cabrera-Barjas, Gustavo and Nešić, Aleksandra and Bravo-Arrepol, Gaston and Rodríguez-Llamazares, Saddys and Valdés, Oscar and Banerjee, Aparna and Castaño, Johanna and Delattre, Cédric",
year = "2021",
abstract = "This study investigated the biocomposite pectin films enriched with murta (Ugni molinae T.) seed polyphenolic extract and reinforced by chitin nanofiber. The structural, morphological, mechanical, barrier, colorimetric, and antioxidant activity of films were evaluated. The obtained data clearly demonstrated that the addition of murta seed extract and the high load of chitin nanofibers (50%) provided more cohesive and dense morphology of films and improved the mechanical resistance and water vapor barrier in comparison to the control pectin film. The antioxidant activity ranged between 71% and 86%, depending on the film formulation and concentration of chitin nanofibers. The presented results highlight the potential use of chitin nanofibers and murta seed extract in the pectin matrix to be applied in functional food coatings and packaging, as a sustainable solution.",
journal = "Molecules",
title = "Bioactive Pectin-Murta (Ugni molinae T.) Seed Extract Films Reinforced with Chitin Fibers",
volume = "26",
number = "24",
pages = "7477",
doi = "10.3390/molecules26247477"
}

Cabrera-Barjas, G., Nešić, A., Bravo-Arrepol, G., Rodríguez-Llamazares, S., Valdés, O., Banerjee, A., Castaño, J.,& Delattre, C.. (2021). Bioactive Pectin-Murta (Ugni molinae T.) Seed Extract Films Reinforced with Chitin Fibers. in Molecules, 26(24), 7477.
https://doi.org/10.3390/molecules26247477

Cabrera-Barjas G, Nešić A, Bravo-Arrepol G, Rodríguez-Llamazares S, Valdés O, Banerjee A, Castaño J, Delattre C. Bioactive Pectin-Murta (Ugni molinae T.) Seed Extract Films Reinforced with Chitin Fibers. in Molecules. 2021;26(24):7477.
doi:10.3390/molecules26247477 .

Cabrera-Barjas, Gustavo, Nešić, Aleksandra, Bravo-Arrepol, Gaston, Rodríguez-Llamazares, Saddys, Valdés, Oscar, Banerjee, Aparna, Castaño, Johanna, Delattre, Cédric, "Bioactive Pectin-Murta (Ugni molinae T.) Seed Extract Films Reinforced with Chitin Fibers" in Molecules, 26, no. 24 (2021):7477,
https://doi.org/10.3390/molecules26247477 . .