Srdić-Rajić, Tatjana

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orcid::0000-0001-9840-6970
  • Srdić-Rajić, Tatjana (8)
  • Srdić, Tatjana (2)

Author's Bibliography

A Model for Potential B-cell Precursors of Broadly Neutralizing HIV-1 Antibodies Selection and Antibody Affinity Maturation

Metlaš, Radmila; Srdić-Rajić, Tatjana

(2019)

TY  - JOUR
AU  - Metlaš, Radmila
AU  - Srdić-Rajić, Tatjana
PY  - 2019
UR  - http://www.aidsreviews.com/resumen.php?id=1472
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8128
AB  - The goal of this report was to propose a model, wherein synergy between the B-cell antigen receptor (BCR) and toll-like receptor (TLR) signaling is involved in the selection of the B-cell precursors of HIV-1 broadly neutralizing antibodies (bnAbs) with long heavy chain complementarity determining regions 3, from immature/ transitional B cells. The model predicts the involvement of Ab/HIV-1 complexes in a way that Ab from the complex binds both BCRs and HIV-1, while on internalization of HIV-1 TLR ligands such as CpG motifs interacts with TLR9. The result of BCR and TLR9 orchestrated signaling is a formation of somatically mutated memory B cells potential precursors of bnAbs. Generated memory B cells continuously exposed to different Ab/HIV-1 complexes can elicit specific bnAb by stochastic somatic hypermutation rather than in the Darwinian process. This new view of the interaction between Ab/HIV-1 complexes and immune system, leading to affinity maturation of the bnAbs in the absence of nominal HIV-1 antigen and BCR interaction, may have implication for the vaccine designed and passive immunization. © 2019, Publicaciones Permanyer. All rights reserved.
T2  - Aids Reviews
T1  - A Model for Potential B-cell Precursors of Broadly Neutralizing HIV-1 Antibodies Selection and Antibody Affinity Maturation
VL  - 21
IS  - 1
SP  - 23
EP  - 27
DO  - 10.24875/AIDSRev.19000028
ER  - 
@article{
author = "Metlaš, Radmila and Srdić-Rajić, Tatjana",
year = "2019",
url = "http://www.aidsreviews.com/resumen.php?id=1472, http://vinar.vin.bg.ac.rs/handle/123456789/8128",
abstract = "The goal of this report was to propose a model, wherein synergy between the B-cell antigen receptor (BCR) and toll-like receptor (TLR) signaling is involved in the selection of the B-cell precursors of HIV-1 broadly neutralizing antibodies (bnAbs) with long heavy chain complementarity determining regions 3, from immature/ transitional B cells. The model predicts the involvement of Ab/HIV-1 complexes in a way that Ab from the complex binds both BCRs and HIV-1, while on internalization of HIV-1 TLR ligands such as CpG motifs interacts with TLR9. The result of BCR and TLR9 orchestrated signaling is a formation of somatically mutated memory B cells potential precursors of bnAbs. Generated memory B cells continuously exposed to different Ab/HIV-1 complexes can elicit specific bnAb by stochastic somatic hypermutation rather than in the Darwinian process. This new view of the interaction between Ab/HIV-1 complexes and immune system, leading to affinity maturation of the bnAbs in the absence of nominal HIV-1 antigen and BCR interaction, may have implication for the vaccine designed and passive immunization. © 2019, Publicaciones Permanyer. All rights reserved.",
journal = "Aids Reviews",
title = "A Model for Potential B-cell Precursors of Broadly Neutralizing HIV-1 Antibodies Selection and Antibody Affinity Maturation",
volume = "21",
number = "1",
pages = "23-27",
doi = "10.24875/AIDSRev.19000028"
}
Metlaš, R.,& Srdić-Rajić, T. (2019). A Model for Potential B-cell Precursors of Broadly Neutralizing HIV-1 Antibodies Selection and Antibody Affinity Maturation.
Aids Reviews, 21(1), 23-27.
https://doi.org/10.24875/AIDSRev.19000028
Metlaš R, Srdić-Rajić T. A Model for Potential B-cell Precursors of Broadly Neutralizing HIV-1 Antibodies Selection and Antibody Affinity Maturation. Aids Reviews. 2019;21(1):23-27
Metlaš Radmila, Srdić-Rajić Tatjana, "A Model for Potential B-cell Precursors of Broadly Neutralizing HIV-1 Antibodies Selection and Antibody Affinity Maturation" Aids Reviews, 21, no. 1 (2019):23-27,
https://doi.org/10.24875/AIDSRev.19000028 .
1

Antibody Epitope Specificity for dsDNA Phosphate Backbone Is an Intrinsic Property of the Heavy Chain Variable Germline Gene Segment Used

Srdić-Rajić, Tatjana; Kohler, Heinz V.; Jurišić, Vladimir; Metlaš, Radmila

(2018)

TY  - JOUR
AU  - Srdić-Rajić, Tatjana
AU  - Kohler, Heinz V.
AU  - Jurišić, Vladimir
AU  - Metlaš, Radmila
PY  - 2018
UR  - https://www.frontiersin.org/article/10.3389/fimmu.2018.02378/full
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7929
AB  - Analysis of protein sequences by the informational spectrum method (ISM) enables characterization of their specificity according to encoded information represented with defined frequency (F). Our previous data showed that F(0.367) is characteristic for variable heavy chain (VH) domains (a combination of variable (V), diversity (D) and joining (J) gene segments) of the anti-phosphocholine (PC) T15 antibodies and mostly dependent on the CDR2 region, a site for PC phosphate group binding. Because the T15 dsDNA-reactive U4 mutant also encodes F(0.367), we hypothesized that the same frequency may also be characteristic for anti-DNA antibodies. Data obtained from an analysis of 60 spontaneously produced anti-DNA antibody VH domain sequences supported our hypothesis only for antibodies, which use V gene segment in germline configuration, such as S57(VH31), MRL-DNA22, and VH11, members of the VH1 (J558) and VH7 (S107) gene families. The important finding is that out of seven V gene segments used by spontaneous anti-DNA antibodies, F(0.367) is only expressed by the germline configuration of these three V gene segments. The data suggest that antibody specificity for the phosphate group moiety delineated as F(0.367) is the intrinsic property of the V germline gene segments used, whereas paratope/epitope interaction with antigens bearing this epitope, such as PC or dsDNA, requires corresponding antibody VH conformation that is susceptible to somatic mutation(s). © 2018 Srdic-Rajic, Kohler, Jurisic and Metlas.
T2  - Frontiers in Immunology
T1  - Antibody Epitope Specificity for dsDNA Phosphate Backbone Is an Intrinsic Property of the Heavy Chain Variable Germline Gene Segment Used
VL  - 9
IS  - OCT
SP  - 2378
DO  - 10.3389/fimmu.2018.02378
ER  - 
@article{
author = "Srdić-Rajić, Tatjana and Kohler, Heinz V. and Jurišić, Vladimir and Metlaš, Radmila",
year = "2018",
url = "https://www.frontiersin.org/article/10.3389/fimmu.2018.02378/full, http://vinar.vin.bg.ac.rs/handle/123456789/7929",
abstract = "Analysis of protein sequences by the informational spectrum method (ISM) enables characterization of their specificity according to encoded information represented with defined frequency (F). Our previous data showed that F(0.367) is characteristic for variable heavy chain (VH) domains (a combination of variable (V), diversity (D) and joining (J) gene segments) of the anti-phosphocholine (PC) T15 antibodies and mostly dependent on the CDR2 region, a site for PC phosphate group binding. Because the T15 dsDNA-reactive U4 mutant also encodes F(0.367), we hypothesized that the same frequency may also be characteristic for anti-DNA antibodies. Data obtained from an analysis of 60 spontaneously produced anti-DNA antibody VH domain sequences supported our hypothesis only for antibodies, which use V gene segment in germline configuration, such as S57(VH31), MRL-DNA22, and VH11, members of the VH1 (J558) and VH7 (S107) gene families. The important finding is that out of seven V gene segments used by spontaneous anti-DNA antibodies, F(0.367) is only expressed by the germline configuration of these three V gene segments. The data suggest that antibody specificity for the phosphate group moiety delineated as F(0.367) is the intrinsic property of the V germline gene segments used, whereas paratope/epitope interaction with antigens bearing this epitope, such as PC or dsDNA, requires corresponding antibody VH conformation that is susceptible to somatic mutation(s). © 2018 Srdic-Rajic, Kohler, Jurisic and Metlas.",
journal = "Frontiers in Immunology",
title = "Antibody Epitope Specificity for dsDNA Phosphate Backbone Is an Intrinsic Property of the Heavy Chain Variable Germline Gene Segment Used",
volume = "9",
number = "OCT",
pages = "2378",
doi = "10.3389/fimmu.2018.02378"
}
Srdić-Rajić, T., Kohler, H. V., Jurišić, V.,& Metlaš, R. (2018). Antibody Epitope Specificity for dsDNA Phosphate Backbone Is an Intrinsic Property of the Heavy Chain Variable Germline Gene Segment Used.
Frontiers in Immunology, 9(OCT), 2378.
https://doi.org/10.3389/fimmu.2018.02378
Srdić-Rajić T, Kohler HV, Jurišić V, Metlaš R. Antibody Epitope Specificity for dsDNA Phosphate Backbone Is an Intrinsic Property of the Heavy Chain Variable Germline Gene Segment Used. Frontiers in Immunology. 2018;9(OCT):2378
Srdić-Rajić Tatjana, Kohler Heinz V., Jurišić Vladimir, Metlaš Radmila, "Antibody Epitope Specificity for dsDNA Phosphate Backbone Is an Intrinsic Property of the Heavy Chain Variable Germline Gene Segment Used" Frontiers in Immunology, 9, no. OCT (2018):2378,
https://doi.org/10.3389/fimmu.2018.02378 .

Cooperation of intrathymic T15 idiotype-bearing B and complementary T cells in ontogeny of natural Treg cells involved in establishment of T15 clonal dominance

Metlaš, Radmila; Srdić-Rajić, Tatjana; Kohler, Heike

(2018)

TY  - JOUR
AU  - Metlaš, Radmila
AU  - Srdić-Rajić, Tatjana
AU  - Kohler, Heike
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0165247818301147
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7793
AB  - The mechanisms for dominant T15 idiotype selection are not well understood, yet the significance of idiotypic regulation has been suggested. We proposed that to become dominant V regions of a given subset of B-1a cell must establish a functional idiotypic network with complementary T cells. Features required for the cells involved in immune network and steps preceding the establishment of clonal dominance are suggested. © 2018
T2  - Immunology Letters
T1  - Cooperation of intrathymic T15 idiotype-bearing B and complementary T cells in ontogeny of natural Treg cells involved in establishment of T15 clonal dominance
VL  - 200
SP  - 52
EP  - 54
DO  - 10.1016/j.imlet.2018.07.002
ER  - 
@article{
author = "Metlaš, Radmila and Srdić-Rajić, Tatjana and Kohler, Heike",
year = "2018",
url = "https://linkinghub.elsevier.com/retrieve/pii/S0165247818301147, http://vinar.vin.bg.ac.rs/handle/123456789/7793",
abstract = "The mechanisms for dominant T15 idiotype selection are not well understood, yet the significance of idiotypic regulation has been suggested. We proposed that to become dominant V regions of a given subset of B-1a cell must establish a functional idiotypic network with complementary T cells. Features required for the cells involved in immune network and steps preceding the establishment of clonal dominance are suggested. © 2018",
journal = "Immunology Letters",
title = "Cooperation of intrathymic T15 idiotype-bearing B and complementary T cells in ontogeny of natural Treg cells involved in establishment of T15 clonal dominance",
volume = "200",
pages = "52-54",
doi = "10.1016/j.imlet.2018.07.002"
}
Metlaš, R., Srdić-Rajić, T.,& Kohler, H. (2018). Cooperation of intrathymic T15 idiotype-bearing B and complementary T cells in ontogeny of natural Treg cells involved in establishment of T15 clonal dominance.
Immunology Letters, 200, 52-54.
https://doi.org/10.1016/j.imlet.2018.07.002
Metlaš R, Srdić-Rajić T, Kohler H. Cooperation of intrathymic T15 idiotype-bearing B and complementary T cells in ontogeny of natural Treg cells involved in establishment of T15 clonal dominance. Immunology Letters. 2018;200:52-54
Metlaš Radmila, Srdić-Rajić Tatjana, Kohler Heike, "Cooperation of intrathymic T15 idiotype-bearing B and complementary T cells in ontogeny of natural Treg cells involved in establishment of T15 clonal dominance" Immunology Letters, 200 (2018):52-54,
https://doi.org/10.1016/j.imlet.2018.07.002 .
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Rilmenidine suppresses proliferation and promotes apoptosis via the mitochondrial pathway in human leukemic K562 cells

Srdić-Rajić, Tatjana; Nikolic, Katarina; Cavic, Milena; Đokić, Ivana; Gemović, Branislava S.; Perović, Vladimir R.; Veljković, Nevena V.

(2016)

TY  - JOUR
AU  - Srdić-Rajić, Tatjana
AU  - Nikolic, Katarina
AU  - Cavic, Milena
AU  - Đokić, Ivana
AU  - Gemović, Branislava S.
AU  - Perović, Vladimir R.
AU  - Veljković, Nevena V.
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/884
AB  - Imidazoline I1 receptor signaling is associated with pathways that regulate cell viability leading to varied cell-type specific phenotypes. We demonstrated that the antihypertensive drug rilmenidine, a selective imidazoline I1 receptor agonist, modulates proliferation and stimulates the proapoptotic protein Bax thus inducing the perturbation of the mitochondrial pathway and apoptosis in human leukemic K562 cells. Rilmenidine acts through a mechanism which involves deactivation of Ras/MAP kinases ERK, p38 and JNK. Moreover, rilmenidine renders K562 cells, which are particularly resistant to chemotherapeutic agents, susceptible to the DNA damaging drug doxorubicin. The rilmenidine co-treatment with doxorubicin reverses G2/M arrest and triggers apoptotic response to DNA damage. Our data offer new insights into the pathways associated with imidazoline I1 receptor activation in K562 cells suggesting rilmenidine as a valuable tool to deepen our understanding of imidazoline I1 receptor signaling in hematologic malignancies and to search for medicinally active agents. (C) 2015 Elsevier B.V. All rights reserved.
T2  - European Journal of Pharmaceutical Sciences
T1  - Rilmenidine suppresses proliferation and promotes apoptosis via the mitochondrial pathway in human leukemic K562 cells
VL  - 81
SP  - 172
EP  - 180
DO  - 10.1016/j.ejps.2015.10.017
ER  - 
@article{
author = "Srdić-Rajić, Tatjana and Nikolic, Katarina and Cavic, Milena and Đokić, Ivana and Gemović, Branislava S. and Perović, Vladimir R. and Veljković, Nevena V.",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/884",
abstract = "Imidazoline I1 receptor signaling is associated with pathways that regulate cell viability leading to varied cell-type specific phenotypes. We demonstrated that the antihypertensive drug rilmenidine, a selective imidazoline I1 receptor agonist, modulates proliferation and stimulates the proapoptotic protein Bax thus inducing the perturbation of the mitochondrial pathway and apoptosis in human leukemic K562 cells. Rilmenidine acts through a mechanism which involves deactivation of Ras/MAP kinases ERK, p38 and JNK. Moreover, rilmenidine renders K562 cells, which are particularly resistant to chemotherapeutic agents, susceptible to the DNA damaging drug doxorubicin. The rilmenidine co-treatment with doxorubicin reverses G2/M arrest and triggers apoptotic response to DNA damage. Our data offer new insights into the pathways associated with imidazoline I1 receptor activation in K562 cells suggesting rilmenidine as a valuable tool to deepen our understanding of imidazoline I1 receptor signaling in hematologic malignancies and to search for medicinally active agents. (C) 2015 Elsevier B.V. All rights reserved.",
journal = "European Journal of Pharmaceutical Sciences",
title = "Rilmenidine suppresses proliferation and promotes apoptosis via the mitochondrial pathway in human leukemic K562 cells",
volume = "81",
pages = "172-180",
doi = "10.1016/j.ejps.2015.10.017"
}
Srdić-Rajić, T., Nikolic, K., Cavic, M., Đokić, I., Gemović, B. S., Perović, V. R.,& Veljković, N. V. (2016). Rilmenidine suppresses proliferation and promotes apoptosis via the mitochondrial pathway in human leukemic K562 cells.
European Journal of Pharmaceutical Sciences, 81, 172-180.
https://doi.org/10.1016/j.ejps.2015.10.017
Srdić-Rajić T, Nikolic K, Cavic M, Đokić I, Gemović BS, Perović VR, Veljković NV. Rilmenidine suppresses proliferation and promotes apoptosis via the mitochondrial pathway in human leukemic K562 cells. European Journal of Pharmaceutical Sciences. 2016;81:172-180
Srdić-Rajić Tatjana, Nikolic Katarina, Cavic Milena, Đokić Ivana, Gemović Branislava S., Perović Vladimir R., Veljković Nevena V., "Rilmenidine suppresses proliferation and promotes apoptosis via the mitochondrial pathway in human leukemic K562 cells" European Journal of Pharmaceutical Sciences, 81 (2016):172-180,
https://doi.org/10.1016/j.ejps.2015.10.017 .
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A combined ligand- and structure-based approach for the identification of rilmenidine-derived compounds which synergize the antitumor effects of doxorubicin

Vucicevic, Jelica; Srdić-Rajić, Tatjana; Pieroni, Marco; Laurila, Jonne M. M.; Perović, Vladimir R.; Tassini, Sabrina; Azzali, Elisa; Costantino, Gabriele; Glišić, Sanja; Agbaba, Danica; Scheinin, Mika; Nikolic, Katarina; Radi, Marco; Veljković, Nevena V.

(2016)

TY  - JOUR
AU  - Vucicevic, Jelica
AU  - Srdić-Rajić, Tatjana
AU  - Pieroni, Marco
AU  - Laurila, Jonne M. M.
AU  - Perović, Vladimir R.
AU  - Tassini, Sabrina
AU  - Azzali, Elisa
AU  - Costantino, Gabriele
AU  - Glišić, Sanja
AU  - Agbaba, Danica
AU  - Scheinin, Mika
AU  - Nikolic, Katarina
AU  - Radi, Marco
AU  - Veljković, Nevena V.
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1110
AB  - The clonidine-like central antihypertensive agent rilmenidine, which has high affinity for I-1-type imidazoline receptors (I-1-IR) was recently found to have cytotoxic effects on cultured cancer cell lines. However, due to its pharmacological effects resulting also from alpha(2)-adrenoceptor activation, rilmenidine cannot be considered a suitable anticancer drug candidate. Here, we report the identification of novel rilmenidine- derived compounds with anticancer potential and devoid of alpha(2)-adrenoceptor effects by means of ligand-and structure-based drug design approaches. Starting from a large virtual library, eleven compounds were selected, synthesized and submitted to biological evaluation. The most active compound 5 exhibited a cytotoxic profile similar to that of rilmenidine, but without appreciable affinity to alpha(2)-adrenoceptors. In addition, compound 5 significantly enhanced the apoptotic response to doxorubicin, and may thus represent an important tool for the development of better adjuvant chemotherapeutic strategies for doxorubicin-insensitive cancers. (C) 2016 Elsevier Ltd. All rights reserved.
T2  - Bioorganic and Medicinal Chemistry
T1  - A combined ligand- and structure-based approach for the identification of rilmenidine-derived compounds which synergize the antitumor effects of doxorubicin
VL  - 24
IS  - 14
SP  - 3174
EP  - 3183
DO  - 10.1016/j.bmc.2016.05.043
ER  - 
@article{
author = "Vucicevic, Jelica and Srdić-Rajić, Tatjana and Pieroni, Marco and Laurila, Jonne M. M. and Perović, Vladimir R. and Tassini, Sabrina and Azzali, Elisa and Costantino, Gabriele and Glišić, Sanja and Agbaba, Danica and Scheinin, Mika and Nikolic, Katarina and Radi, Marco and Veljković, Nevena V.",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1110",
abstract = "The clonidine-like central antihypertensive agent rilmenidine, which has high affinity for I-1-type imidazoline receptors (I-1-IR) was recently found to have cytotoxic effects on cultured cancer cell lines. However, due to its pharmacological effects resulting also from alpha(2)-adrenoceptor activation, rilmenidine cannot be considered a suitable anticancer drug candidate. Here, we report the identification of novel rilmenidine- derived compounds with anticancer potential and devoid of alpha(2)-adrenoceptor effects by means of ligand-and structure-based drug design approaches. Starting from a large virtual library, eleven compounds were selected, synthesized and submitted to biological evaluation. The most active compound 5 exhibited a cytotoxic profile similar to that of rilmenidine, but without appreciable affinity to alpha(2)-adrenoceptors. In addition, compound 5 significantly enhanced the apoptotic response to doxorubicin, and may thus represent an important tool for the development of better adjuvant chemotherapeutic strategies for doxorubicin-insensitive cancers. (C) 2016 Elsevier Ltd. All rights reserved.",
journal = "Bioorganic and Medicinal Chemistry",
title = "A combined ligand- and structure-based approach for the identification of rilmenidine-derived compounds which synergize the antitumor effects of doxorubicin",
volume = "24",
number = "14",
pages = "3174-3183",
doi = "10.1016/j.bmc.2016.05.043"
}
Vucicevic, J., Srdić-Rajić, T., Pieroni, M., Laurila, J. M. M., Perović, V. R., Tassini, S., Azzali, E., Costantino, G., Glišić, S., Agbaba, D., Scheinin, M., Nikolic, K., Radi, M.,& Veljković, N. V. (2016). A combined ligand- and structure-based approach for the identification of rilmenidine-derived compounds which synergize the antitumor effects of doxorubicin.
Bioorganic and Medicinal Chemistry, 24(14), 3174-3183.
https://doi.org/10.1016/j.bmc.2016.05.043
Vucicevic J, Srdić-Rajić T, Pieroni M, Laurila JMM, Perović VR, Tassini S, Azzali E, Costantino G, Glišić S, Agbaba D, Scheinin M, Nikolic K, Radi M, Veljković NV. A combined ligand- and structure-based approach for the identification of rilmenidine-derived compounds which synergize the antitumor effects of doxorubicin. Bioorganic and Medicinal Chemistry. 2016;24(14):3174-3183
Vucicevic Jelica, Srdić-Rajić Tatjana, Pieroni Marco, Laurila Jonne M. M., Perović Vladimir R., Tassini Sabrina, Azzali Elisa, Costantino Gabriele, Glišić Sanja, Agbaba Danica, Scheinin Mika, Nikolic Katarina, Radi Marco, Veljković Nevena V., "A combined ligand- and structure-based approach for the identification of rilmenidine-derived compounds which synergize the antitumor effects of doxorubicin" Bioorganic and Medicinal Chemistry, 24, no. 14 (2016):3174-3183,
https://doi.org/10.1016/j.bmc.2016.05.043 .
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Imidazoline-1 Receptor Ligands as Apoptotic Agents: Pharmacophore Modeling and Virtual Docking Study

Nikolic, Katarina; Veljković, Nevena V.; Gemović, Branislava S.; Srdić-Rajić, Tatjana; Agbaba, Danica

(2013)

TY  - JOUR
AU  - Nikolic, Katarina
AU  - Veljković, Nevena V.
AU  - Gemović, Branislava S.
AU  - Srdić-Rajić, Tatjana
AU  - Agbaba, Danica
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5398
AB  - The group of imidazoline-1 receptors (I-1-IR) agonists encompasses drugs are currently used in treatment of high blood pressure and hyperglycemia. The I-1-IR protein structures have not been determined yet, but Nischarin protein that binds numerous imidazoline ligands inducing initiation of various cell-signaling cascades, including apoptosis, is identified as strong I-1-IR candidate. In this study we examined apoptotic activity of rilmenidine (potent I-1-IR agonist), moxonidine (moderate I-1-IR agonist), and efaroxan (I-1-IR partial agonist) on cancer cell line (K562) expressing Nischarin. The Nischarine domains mapping was performed by use of the Informational Spectrum Method (ISM). The 3D-Quantitative Structure-Activity Relationship (3D-QSAR) and virtual docking studies of 29 I-1-IR ligands (agonists, partial agonists, and antagonists) were carried out on I-1-IR receptors binding affinities. The 3D-QSAR study defined 3D-pharmacophore models for I-1-IR agonistic and I-1-IR antagonistic activity and created regression model for prediction of I-1-IR activity of novel compounds. The 3D-QSAR models were applied for design and evaluation of novel I-1-IR agonists and I-1-IR antagonists. The most promising I-1-IR ligands with enhanced activities than parent compounds were proposed for synthesis. The results of 3D-QSAR, ISM, and virtual docking studies were in perfect agreement and allowed precise definition of binding mode of I-1-IR agonists (Arg 758, Arg 866, Val 981, and Glu 1057) and significantly different binding modes of I-1-IR antagonists or partial I-1-IR agonists. The performed theoretical study provides reliable system for evaluation of I-1-IR agonistic and I-1-IR antagonistic activity of novel I-1-IR ligands, as drug candidates with anticancer activities.
T2  - Combinatorial Chemistry and High Throughput Screening
T1  - Imidazoline-1 Receptor Ligands as Apoptotic Agents: Pharmacophore Modeling and Virtual Docking Study
VL  - 16
IS  - 4
SP  - 298
EP  - 319
DO  - 10.2174/1386207311316040004
ER  - 
@article{
author = "Nikolic, Katarina and Veljković, Nevena V. and Gemović, Branislava S. and Srdić-Rajić, Tatjana and Agbaba, Danica",
year = "2013",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5398",
abstract = "The group of imidazoline-1 receptors (I-1-IR) agonists encompasses drugs are currently used in treatment of high blood pressure and hyperglycemia. The I-1-IR protein structures have not been determined yet, but Nischarin protein that binds numerous imidazoline ligands inducing initiation of various cell-signaling cascades, including apoptosis, is identified as strong I-1-IR candidate. In this study we examined apoptotic activity of rilmenidine (potent I-1-IR agonist), moxonidine (moderate I-1-IR agonist), and efaroxan (I-1-IR partial agonist) on cancer cell line (K562) expressing Nischarin. The Nischarine domains mapping was performed by use of the Informational Spectrum Method (ISM). The 3D-Quantitative Structure-Activity Relationship (3D-QSAR) and virtual docking studies of 29 I-1-IR ligands (agonists, partial agonists, and antagonists) were carried out on I-1-IR receptors binding affinities. The 3D-QSAR study defined 3D-pharmacophore models for I-1-IR agonistic and I-1-IR antagonistic activity and created regression model for prediction of I-1-IR activity of novel compounds. The 3D-QSAR models were applied for design and evaluation of novel I-1-IR agonists and I-1-IR antagonists. The most promising I-1-IR ligands with enhanced activities than parent compounds were proposed for synthesis. The results of 3D-QSAR, ISM, and virtual docking studies were in perfect agreement and allowed precise definition of binding mode of I-1-IR agonists (Arg 758, Arg 866, Val 981, and Glu 1057) and significantly different binding modes of I-1-IR antagonists or partial I-1-IR agonists. The performed theoretical study provides reliable system for evaluation of I-1-IR agonistic and I-1-IR antagonistic activity of novel I-1-IR ligands, as drug candidates with anticancer activities.",
journal = "Combinatorial Chemistry and High Throughput Screening",
title = "Imidazoline-1 Receptor Ligands as Apoptotic Agents: Pharmacophore Modeling and Virtual Docking Study",
volume = "16",
number = "4",
pages = "298-319",
doi = "10.2174/1386207311316040004"
}
Nikolic, K., Veljković, N. V., Gemović, B. S., Srdić-Rajić, T.,& Agbaba, D. (2013). Imidazoline-1 Receptor Ligands as Apoptotic Agents: Pharmacophore Modeling and Virtual Docking Study.
Combinatorial Chemistry and High Throughput Screening, 16(4), 298-319.
https://doi.org/10.2174/1386207311316040004
Nikolic K, Veljković NV, Gemović BS, Srdić-Rajić T, Agbaba D. Imidazoline-1 Receptor Ligands as Apoptotic Agents: Pharmacophore Modeling and Virtual Docking Study. Combinatorial Chemistry and High Throughput Screening. 2013;16(4):298-319
Nikolic Katarina, Veljković Nevena V., Gemović Branislava S., Srdić-Rajić Tatjana, Agbaba Danica, "Imidazoline-1 Receptor Ligands as Apoptotic Agents: Pharmacophore Modeling and Virtual Docking Study" Combinatorial Chemistry and High Throughput Screening, 16, no. 4 (2013):298-319,
https://doi.org/10.2174/1386207311316040004 .
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Phosphocholine-binding antibody activities are hierarchically encoded in the sequence of the heavy-chain variable region: dominance of self-association activity in the T15 idiotype

Srdić-Rajić, Tatjana; Kekovic, Goran; Davidovic, Dragomir M.; Metlaš, Radmila

(2013)

TY  - JOUR
AU  - Srdić-Rajić, Tatjana
AU  - Kekovic, Goran
AU  - Davidovic, Dragomir M.
AU  - Metlaš, Radmila
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5519
AB  - A physical and mathematical model identifies similarities between phosphocholine-binding antibodies.A methodology based on the representation of each amino acid of a protein sequence by the electron-ion interaction potential and subsequent analysis by signal processing was used to determine the characteristic or common frequency (in Hz) that reflects the biological activity shared among phosphocholine (PC)-binding antibodies. The common frequency for the variable portion of the heavy chain (VH) of the PC-specific antibodies is found to be at f 0.37 Hz. The VH sequences of the PC-binding antibodies exhibit three subsites for the PC moiety where hypervariable region 2 (CDR2) plays a role in the interaction with the phosphate group. Mutations in this VH region have an impact on the ability of mutant variants to bind PC and its carrier molecule, as well as on the characteristic frequency shift toward f 0.12 Hz for mutants failing to bind both hapten and carrier. The VH sequence of mutants that retain the ability to bind PC still shows f 0.37 Hz, suggesting that this frequency determines PC binding. However, this statement was not confirmed as mutation in another PC subsite impairs PC binding but retains both the phosphate-group recognition and the frequency at f 0.37 Hz. Herein, this finding is discussed to promote the idea that the VH sequence of the PC-binding antibodies encodes the subsite for phosphate-group binding as a dominant functional activity and that only CDR2 of the T15-idiotype antibodies together with FR3 region form an autonomous self-association function represented by the T15VH5073 peptide with f 0.370.05 Hz. Thus, these data confirmed that T15VH50-73 peptide might be used in superantibody technology.
T2  - International Immunology
T1  - Phosphocholine-binding antibody activities are hierarchically encoded in the sequence of the heavy-chain variable region: dominance of self-association activity in the T15 idiotype
VL  - 25
IS  - 6
SP  - 345
EP  - 352
DO  - 10.1093/intimm/dxs156
ER  - 
@article{
author = "Srdić-Rajić, Tatjana and Kekovic, Goran and Davidovic, Dragomir M. and Metlaš, Radmila",
year = "2013",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5519",
abstract = "A physical and mathematical model identifies similarities between phosphocholine-binding antibodies.A methodology based on the representation of each amino acid of a protein sequence by the electron-ion interaction potential and subsequent analysis by signal processing was used to determine the characteristic or common frequency (in Hz) that reflects the biological activity shared among phosphocholine (PC)-binding antibodies. The common frequency for the variable portion of the heavy chain (VH) of the PC-specific antibodies is found to be at f 0.37 Hz. The VH sequences of the PC-binding antibodies exhibit three subsites for the PC moiety where hypervariable region 2 (CDR2) plays a role in the interaction with the phosphate group. Mutations in this VH region have an impact on the ability of mutant variants to bind PC and its carrier molecule, as well as on the characteristic frequency shift toward f 0.12 Hz for mutants failing to bind both hapten and carrier. The VH sequence of mutants that retain the ability to bind PC still shows f 0.37 Hz, suggesting that this frequency determines PC binding. However, this statement was not confirmed as mutation in another PC subsite impairs PC binding but retains both the phosphate-group recognition and the frequency at f 0.37 Hz. Herein, this finding is discussed to promote the idea that the VH sequence of the PC-binding antibodies encodes the subsite for phosphate-group binding as a dominant functional activity and that only CDR2 of the T15-idiotype antibodies together with FR3 region form an autonomous self-association function represented by the T15VH5073 peptide with f 0.370.05 Hz. Thus, these data confirmed that T15VH50-73 peptide might be used in superantibody technology.",
journal = "International Immunology",
title = "Phosphocholine-binding antibody activities are hierarchically encoded in the sequence of the heavy-chain variable region: dominance of self-association activity in the T15 idiotype",
volume = "25",
number = "6",
pages = "345-352",
doi = "10.1093/intimm/dxs156"
}
Srdić-Rajić, T., Kekovic, G., Davidovic, D. M.,& Metlaš, R. (2013). Phosphocholine-binding antibody activities are hierarchically encoded in the sequence of the heavy-chain variable region: dominance of self-association activity in the T15 idiotype.
International Immunology, 25(6), 345-352.
https://doi.org/10.1093/intimm/dxs156
Srdić-Rajić T, Kekovic G, Davidovic DM, Metlaš R. Phosphocholine-binding antibody activities are hierarchically encoded in the sequence of the heavy-chain variable region: dominance of self-association activity in the T15 idiotype. International Immunology. 2013;25(6):345-352
Srdić-Rajić Tatjana, Kekovic Goran, Davidovic Dragomir M., Metlaš Radmila, "Phosphocholine-binding antibody activities are hierarchically encoded in the sequence of the heavy-chain variable region: dominance of self-association activity in the T15 idiotype" International Immunology, 25, no. 6 (2013):345-352,
https://doi.org/10.1093/intimm/dxs156 .
1
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3

Naturally occurring V region connected antibodies inhibit anti-dsDNA antibody reactivity with dsDNA

Srdić-Rajić, Tatjana; Jurišić, Vladimir; Andrejevic, Sladjana; Bonaci-Nikolic, Branka; Bowker, Timothy; Concas, Daniela; Metlaš, Radmila

(2012)

TY  - JOUR
AU  - Srdić-Rajić, Tatjana
AU  - Jurišić, Vladimir
AU  - Andrejevic, Sladjana
AU  - Bonaci-Nikolic, Branka
AU  - Bowker, Timothy
AU  - Concas, Daniela
AU  - Metlaš, Radmila
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4657
AB  - The production of autoantibodies against a vast array of self antigens, most notably double stranded (ds) DNA, characterized systemic lupus erythematosus (SLE). The purpose of this work is to study specific Ig fractions isolated from normal human serum (NHS) and their effect on the binding of anti-double-stranded deoxyribonucleic acid (dsDNA) antibodies (Abs) to dsDNA. A fraction named immunoglobulin G (IgG)-reactive IgG was purified from total NHS IgG by absorption onto (CNBr)-activated Sepharose 4B linked to intact IgG molecules (IgG-Sepharose column). IgG-reactive IgG was co-incubated with systemic lupus erythematosus (SLE) patients serum and binding of the anti-dsDNA Abs to dsDNA was measured by enzyme-linked immunosorbent assay (ELISA). Co-incubation of SLE patients serum with IgG-reactive IgG resulted in a dose-dependent reduction in binding of anti-dsDNA Abs to dsDNA. A reduction greater than 70% was observed at a concentration of 300 mu g of IgG-reactive IgG per mL of a 400-fold diluted SLE patients serum whereas total NHS IgG, at the same concentration, resulted in a 10% reduction in binding. The purification process used to isolate IgG-reactive IgG was based on interactions between intact Ig rather than on interactions between F(ab)(2) portions. IgG(2) is the predominant immunoglobulin (Ig) subclass in IgG-reactive IgG. Thus, IgG(2) might have an important role in the connectivity characteristics of NHS IgG. The capacity of IgG-reactive IgG to inhibit anti-DNA Ab binding to dsDNA may have potential application in the treatment of SLE. This targeted biological approach may provide an alternative strategy to immunosuppressants. (C) 2011 Elsevier GmbH. All rights reserved.
T2  - Immunobiology
T1  - Naturally occurring V region connected antibodies inhibit anti-dsDNA antibody reactivity with dsDNA
VL  - 217
IS  - 1
SP  - 111
EP  - 117
DO  - 10.1016/j.imbio.2011.07.026
ER  - 
@article{
author = "Srdić-Rajić, Tatjana and Jurišić, Vladimir and Andrejevic, Sladjana and Bonaci-Nikolic, Branka and Bowker, Timothy and Concas, Daniela and Metlaš, Radmila",
year = "2012",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4657",
abstract = "The production of autoantibodies against a vast array of self antigens, most notably double stranded (ds) DNA, characterized systemic lupus erythematosus (SLE). The purpose of this work is to study specific Ig fractions isolated from normal human serum (NHS) and their effect on the binding of anti-double-stranded deoxyribonucleic acid (dsDNA) antibodies (Abs) to dsDNA. A fraction named immunoglobulin G (IgG)-reactive IgG was purified from total NHS IgG by absorption onto (CNBr)-activated Sepharose 4B linked to intact IgG molecules (IgG-Sepharose column). IgG-reactive IgG was co-incubated with systemic lupus erythematosus (SLE) patients serum and binding of the anti-dsDNA Abs to dsDNA was measured by enzyme-linked immunosorbent assay (ELISA). Co-incubation of SLE patients serum with IgG-reactive IgG resulted in a dose-dependent reduction in binding of anti-dsDNA Abs to dsDNA. A reduction greater than 70% was observed at a concentration of 300 mu g of IgG-reactive IgG per mL of a 400-fold diluted SLE patients serum whereas total NHS IgG, at the same concentration, resulted in a 10% reduction in binding. The purification process used to isolate IgG-reactive IgG was based on interactions between intact Ig rather than on interactions between F(ab)(2) portions. IgG(2) is the predominant immunoglobulin (Ig) subclass in IgG-reactive IgG. Thus, IgG(2) might have an important role in the connectivity characteristics of NHS IgG. The capacity of IgG-reactive IgG to inhibit anti-DNA Ab binding to dsDNA may have potential application in the treatment of SLE. This targeted biological approach may provide an alternative strategy to immunosuppressants. (C) 2011 Elsevier GmbH. All rights reserved.",
journal = "Immunobiology",
title = "Naturally occurring V region connected antibodies inhibit anti-dsDNA antibody reactivity with dsDNA",
volume = "217",
number = "1",
pages = "111-117",
doi = "10.1016/j.imbio.2011.07.026"
}
Srdić-Rajić, T., Jurišić, V., Andrejevic, S., Bonaci-Nikolic, B., Bowker, T., Concas, D.,& Metlaš, R. (2012). Naturally occurring V region connected antibodies inhibit anti-dsDNA antibody reactivity with dsDNA.
Immunobiology, 217(1), 111-117.
https://doi.org/10.1016/j.imbio.2011.07.026
Srdić-Rajić T, Jurišić V, Andrejevic S, Bonaci-Nikolic B, Bowker T, Concas D, Metlaš R. Naturally occurring V region connected antibodies inhibit anti-dsDNA antibody reactivity with dsDNA. Immunobiology. 2012;217(1):111-117
Srdić-Rajić Tatjana, Jurišić Vladimir, Andrejevic Sladjana, Bonaci-Nikolic Branka, Bowker Timothy, Concas Daniela, Metlaš Radmila, "Naturally occurring V region connected antibodies inhibit anti-dsDNA antibody reactivity with dsDNA" Immunobiology, 217, no. 1 (2012):111-117,
https://doi.org/10.1016/j.imbio.2011.07.026 .
3
3
3

Characteristics of novel myeloid precursor cell line, PC-MDS, established from a bone marrow of the patient with therapy-related myelodysplastic syndrome

Bogdanovic, Gordana; Jurišić, Vladimir; Kraguljac, Nada; Mrđanović, Jasminka Ž.; Jakimov, Dimitar; Krtolica-Žikić, Koviljka; Krajnović, Milena M.; Magic, Zvonko; Stojiljkovic, Bratislav; Andrijevic, Ljiljana; Srdić, Tatjana; Baltic, Mirjana; Popovic, Stevan

(2007)

TY  - JOUR
AU  - Bogdanovic, Gordana
AU  - Jurišić, Vladimir
AU  - Kraguljac, Nada
AU  - Mrđanović, Jasminka Ž.
AU  - Jakimov, Dimitar
AU  - Krtolica-Žikić, Koviljka
AU  - Krajnović, Milena M.
AU  - Magic, Zvonko
AU  - Stojiljkovic, Bratislav
AU  - Andrijevic, Ljiljana
AU  - Srdić, Tatjana
AU  - Baltic, Mirjana
AU  - Popovic, Stevan
PY  - 2007
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3219
AB  - We report on characteristics of the first human cell line, PC-MDS, derived from a bone marrow of a patient with therapy-related myelodysplastic syndrome (t-MDS) who had no overt post-MDS leukemia. Classic cytology analyses, immunophenotyping, cytogenetic and molecular genetic procedures were used for characterization of the cell line. PC-MDS cells are positive for the expression of CD13, CD15, CD30, CD33, and CD45 antigen. Positive cytochemical staining and immunophenotype analyses indicated that PC-MDS cells have some characteristics of the early myeloid precursor cell. The karyotype analysis of PC-MDS cell line revealed various numerical and structural changes including those typically associated with t-MDS: del(5)(q13)[7], der(5)t(5;11)(p11;q11)[13], -7[6], del(7)(q31)[2], +20[3], -20[4]. Evaluation of methylation status in a promoter region of p 15, p 16 and MGMT genes showed biallelic hypermethylation pattern of 5 promoter region only in MGMT gene. PC-MDS is the first t-MDS derived cell line, and based on its immunological, cytogenetic and molecular characterization could be a new tool in evaluation of complex biology of MDS and a model for methylation studies. (c) 2007 Elsevier Ltd. All rights reserved.
T2  - Leukemia Research
T1  - Characteristics of novel myeloid precursor cell line, PC-MDS, established from a bone marrow of the patient with therapy-related myelodysplastic syndrome
VL  - 31
IS  - 8
SP  - 1097
EP  - 1105
DO  - 10.1016/j.leukres.2007.01.012
ER  - 
@article{
author = "Bogdanovic, Gordana and Jurišić, Vladimir and Kraguljac, Nada and Mrđanović, Jasminka Ž. and Jakimov, Dimitar and Krtolica-Žikić, Koviljka and Krajnović, Milena M. and Magic, Zvonko and Stojiljkovic, Bratislav and Andrijevic, Ljiljana and Srdić, Tatjana and Baltic, Mirjana and Popovic, Stevan",
year = "2007",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3219",
abstract = "We report on characteristics of the first human cell line, PC-MDS, derived from a bone marrow of a patient with therapy-related myelodysplastic syndrome (t-MDS) who had no overt post-MDS leukemia. Classic cytology analyses, immunophenotyping, cytogenetic and molecular genetic procedures were used for characterization of the cell line. PC-MDS cells are positive for the expression of CD13, CD15, CD30, CD33, and CD45 antigen. Positive cytochemical staining and immunophenotype analyses indicated that PC-MDS cells have some characteristics of the early myeloid precursor cell. The karyotype analysis of PC-MDS cell line revealed various numerical and structural changes including those typically associated with t-MDS: del(5)(q13)[7], der(5)t(5;11)(p11;q11)[13], -7[6], del(7)(q31)[2], +20[3], -20[4]. Evaluation of methylation status in a promoter region of p 15, p 16 and MGMT genes showed biallelic hypermethylation pattern of 5 promoter region only in MGMT gene. PC-MDS is the first t-MDS derived cell line, and based on its immunological, cytogenetic and molecular characterization could be a new tool in evaluation of complex biology of MDS and a model for methylation studies. (c) 2007 Elsevier Ltd. All rights reserved.",
journal = "Leukemia Research",
title = "Characteristics of novel myeloid precursor cell line, PC-MDS, established from a bone marrow of the patient with therapy-related myelodysplastic syndrome",
volume = "31",
number = "8",
pages = "1097-1105",
doi = "10.1016/j.leukres.2007.01.012"
}
Bogdanovic, G., Jurišić, V., Kraguljac, N., Mrđanović, J. Ž., Jakimov, D., Krtolica-Žikić, K., Krajnović, M. M., Magic, Z., Stojiljkovic, B., Andrijevic, L., Srdić, T., Baltic, M.,& Popovic, S. (2007). Characteristics of novel myeloid precursor cell line, PC-MDS, established from a bone marrow of the patient with therapy-related myelodysplastic syndrome.
Leukemia Research, 31(8), 1097-1105.
https://doi.org/10.1016/j.leukres.2007.01.012
Bogdanovic G, Jurišić V, Kraguljac N, Mrđanović JŽ, Jakimov D, Krtolica-Žikić K, Krajnović MM, Magic Z, Stojiljkovic B, Andrijevic L, Srdić T, Baltic M, Popovic S. Characteristics of novel myeloid precursor cell line, PC-MDS, established from a bone marrow of the patient with therapy-related myelodysplastic syndrome. Leukemia Research. 2007;31(8):1097-1105
Bogdanovic Gordana, Jurišić Vladimir, Kraguljac Nada, Mrđanović Jasminka Ž., Jakimov Dimitar, Krtolica-Žikić Koviljka, Krajnović Milena M., Magic Zvonko, Stojiljkovic Bratislav, Andrijevic Ljiljana, Srdić Tatjana, Baltic Mirjana, Popovic Stevan, "Characteristics of novel myeloid precursor cell line, PC-MDS, established from a bone marrow of the patient with therapy-related myelodysplastic syndrome" Leukemia Research, 31, no. 8 (2007):1097-1105,
https://doi.org/10.1016/j.leukres.2007.01.012 .
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9

Anti-IgG antibodies from sera of healthy individuals neutralize HIV-1 primary isolates

Metlaš, Radmila; Srdić, Tatjana; Vejkovic, Veljko

(2007)

TY  - JOUR
AU  - Metlaš, Radmila
AU  - Srdić, Tatjana
AU  - Vejkovic, Veljko
PY  - 2007
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3168
AB  - Immunoglobulins (Ig) of pooled healthy human sera were purified by affinity chromatography based on their reactivity with human IgG. This Ig fraction represent connected, natural antibodies (NAbs) and here are denoted as antiIgG antibodies. The data revealed that IgG, IgA and IgM isotypes arc constituents of anti-IgG fraction. The ability of antiIgG antibodies to prevent infection of PBMC by HIV-1 was demonstrated. They exhibited different neutralizing activity depending on the phenotype of the tested virus. The efficacy of neutralization was comparable to monoclonal antibodies (MAbs) IgG1b12 at least for the HIV-1 (92HT593B) Strain. These studies suggest that connected antibodies thus, constituents of immune network, could prevent infection by HIV-1. NAbs as essential components of therapeutic molecules of intravenous Ig (IVIg) have a beneficial effect on variety of immunological disorders by affecting the structure, function and dynamics of the immune network. Since, hallmark of HIV-1 infection are immunological disorders we hypothesizes that they might be corrected to some extend by anti-IgG antibodies.
T2  - Current Hiv Research
T1  - Anti-IgG antibodies from sera of healthy individuals neutralize HIV-1 primary isolates
VL  - 5
IS  - 2
SP  - 261
EP  - 265
DO  - 10.2174/157016207780077093
ER  - 
@article{
author = "Metlaš, Radmila and Srdić, Tatjana and Vejkovic, Veljko",
year = "2007",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3168",
abstract = "Immunoglobulins (Ig) of pooled healthy human sera were purified by affinity chromatography based on their reactivity with human IgG. This Ig fraction represent connected, natural antibodies (NAbs) and here are denoted as antiIgG antibodies. The data revealed that IgG, IgA and IgM isotypes arc constituents of anti-IgG fraction. The ability of antiIgG antibodies to prevent infection of PBMC by HIV-1 was demonstrated. They exhibited different neutralizing activity depending on the phenotype of the tested virus. The efficacy of neutralization was comparable to monoclonal antibodies (MAbs) IgG1b12 at least for the HIV-1 (92HT593B) Strain. These studies suggest that connected antibodies thus, constituents of immune network, could prevent infection by HIV-1. NAbs as essential components of therapeutic molecules of intravenous Ig (IVIg) have a beneficial effect on variety of immunological disorders by affecting the structure, function and dynamics of the immune network. Since, hallmark of HIV-1 infection are immunological disorders we hypothesizes that they might be corrected to some extend by anti-IgG antibodies.",
journal = "Current Hiv Research",
title = "Anti-IgG antibodies from sera of healthy individuals neutralize HIV-1 primary isolates",
volume = "5",
number = "2",
pages = "261-265",
doi = "10.2174/157016207780077093"
}
Metlaš, R., Srdić, T.,& Vejkovic, V. (2007). Anti-IgG antibodies from sera of healthy individuals neutralize HIV-1 primary isolates.
Current Hiv Research, 5(2), 261-265.
https://doi.org/10.2174/157016207780077093
Metlaš R, Srdić T, Vejkovic V. Anti-IgG antibodies from sera of healthy individuals neutralize HIV-1 primary isolates. Current Hiv Research. 2007;5(2):261-265
Metlaš Radmila, Srdić Tatjana, Vejkovic Veljko, "Anti-IgG antibodies from sera of healthy individuals neutralize HIV-1 primary isolates" Current Hiv Research, 5, no. 2 (2007):261-265,
https://doi.org/10.2174/157016207780077093 .
4
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5