TY  - JOUR
AU  - Banjac, Katarina
AU  - Obradović, Milan
AU  - Zafirović, Sonja
AU  - Essack, Magbubah
AU  - Gluvić, Zoran
AU  - Šunderić, Miloš
AU  - Nedić, Olgica
AU  - Isenović, Esma R.
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13184
AB  - Background We previously demonstrated that insulin-like growth factor-1 (IGF-1) regulates sodium/potassium adenosine triphosphatase (Na+/K+-ATPase) in vascular smooth muscle cells (VSMC) via phosphatidylinositol-3 kinase (PI3K). Taking into account that others’ work show that IGF-1 activates the PI3K/protein kinase B (Akt) signaling pathway in many different cells, we here further questioned if the Akt/mammalian target of rapamycin (mTOR)/ribosomal protein p70 S6 kinase (S6K) pathway stimulates Na+/K+-ATPase, an essential protein for maintaining normal heart function. Methods and results There were 14 adult male Wistar rats, half of whom received bolus injections of IGF-1 (50 μg/kg) for 24 h. We evaluated cardiac Na+/K+-ATPase expression, activity, and serum IGF-1 levels. Additionally, we examined the phosphorylated forms of the following proteins: insulin receptor substrate (IRS), phosphoinositide-dependent kinase-1 (PDK-1), Akt, mTOR, S6K, and α subunit of Na+/K+-ATPase. Additionally, the mRNA expression of the Na+/K+-ATPase α1 subunit was evaluated. Treatment with IGF-1 increases levels of serum IGF-1 and stimulates Na+/K+-ATPase activity, phosphorylation of α subunit of Na+/K+-ATPase on Ser23, and protein expression of α2 subunit. Furthermore, IGF-1 treatment increased phosphorylation of IRS-1 on Tyr1222, Akt on Ser473, PDK-1 on Ser241, mTOR on Ser2481 and Ser2448, and S6K on Thr421/Ser424. The concentration of IGF-1 in serum positively correlates with Na+/K+-ATPase activity and the phosphorylated form of mTOR (Ser2448), while Na+/K+-ATPase activity positively correlates with the phosphorylated form of IRS-1 (Tyr1222) and mTOR (Ser2448). Conclusion These results indicate that the Akt/mTOR/S6K signalling pathway may be involved in the IGF-1 regulating cardiac Na+/K+-ATPase expression and activity
T2  - Molecular Biology Reports
T1  - The involvement of Akt, mTOR, and S6K in the in vivo effect of IGF-1 on the regulation of rat cardiac Na+/K+-ATPase
VL  - 51
IS  - 1
DO  - 10.1007/s11033-024-09451-3
ER  - 

@article{
author = "Banjac, Katarina and Obradović, Milan and Zafirović, Sonja and Essack, Magbubah and Gluvić, Zoran and Šunderić, Miloš and Nedić, Olgica and Isenović, Esma R.",
year = "2024",
abstract = "Background We previously demonstrated that insulin-like growth factor-1 (IGF-1) regulates sodium/potassium adenosine triphosphatase (Na+/K+-ATPase) in vascular smooth muscle cells (VSMC) via phosphatidylinositol-3 kinase (PI3K). Taking into account that others’ work show that IGF-1 activates the PI3K/protein kinase B (Akt) signaling pathway in many different cells, we here further questioned if the Akt/mammalian target of rapamycin (mTOR)/ribosomal protein p70 S6 kinase (S6K) pathway stimulates Na+/K+-ATPase, an essential protein for maintaining normal heart function. Methods and results There were 14 adult male Wistar rats, half of whom received bolus injections of IGF-1 (50 μg/kg) for 24 h. We evaluated cardiac Na+/K+-ATPase expression, activity, and serum IGF-1 levels. Additionally, we examined the phosphorylated forms of the following proteins: insulin receptor substrate (IRS), phosphoinositide-dependent kinase-1 (PDK-1), Akt, mTOR, S6K, and α subunit of Na+/K+-ATPase. Additionally, the mRNA expression of the Na+/K+-ATPase α1 subunit was evaluated. Treatment with IGF-1 increases levels of serum IGF-1 and stimulates Na+/K+-ATPase activity, phosphorylation of α subunit of Na+/K+-ATPase on Ser23, and protein expression of α2 subunit. Furthermore, IGF-1 treatment increased phosphorylation of IRS-1 on Tyr1222, Akt on Ser473, PDK-1 on Ser241, mTOR on Ser2481 and Ser2448, and S6K on Thr421/Ser424. The concentration of IGF-1 in serum positively correlates with Na+/K+-ATPase activity and the phosphorylated form of mTOR (Ser2448), while Na+/K+-ATPase activity positively correlates with the phosphorylated form of IRS-1 (Tyr1222) and mTOR (Ser2448). Conclusion These results indicate that the Akt/mTOR/S6K signalling pathway may be involved in the IGF-1 regulating cardiac Na+/K+-ATPase expression and activity",
journal = "Molecular Biology Reports",
title = "The involvement of Akt, mTOR, and S6K in the in vivo effect of IGF-1 on the regulation of rat cardiac Na+/K+-ATPase",
volume = "51",
number = "1",
doi = "10.1007/s11033-024-09451-3"
}

Banjac, K., Obradović, M., Zafirović, S., Essack, M., Gluvić, Z., Šunderić, M., Nedić, O.,& Isenović, E. R.. (2024). The involvement of Akt, mTOR, and S6K in the in vivo effect of IGF-1 on the regulation of rat cardiac Na+/K+-ATPase. in Molecular Biology Reports, 51(1).
https://doi.org/10.1007/s11033-024-09451-3

Banjac K, Obradović M, Zafirović S, Essack M, Gluvić Z, Šunderić M, Nedić O, Isenović ER. The involvement of Akt, mTOR, and S6K in the in vivo effect of IGF-1 on the regulation of rat cardiac Na+/K+-ATPase. in Molecular Biology Reports. 2024;51(1).
doi:10.1007/s11033-024-09451-3 .

Banjac, Katarina, Obradović, Milan, Zafirović, Sonja, Essack, Magbubah, Gluvić, Zoran, Šunderić, Miloš, Nedić, Olgica, Isenović, Esma R., "The involvement of Akt, mTOR, and S6K in the in vivo effect of IGF-1 on the regulation of rat cardiac Na+/K+-ATPase" in Molecular Biology Reports, 51, no. 1 (2024),
https://doi.org/10.1007/s11033-024-09451-3 . .

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Sudar-Milovanović, Emina
AU  - Gluvić, Zoran
AU  - Banjac, Katarina
AU  - Rizzo, Manfredi
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10756
AB  - Cardiometabolic diseases (CMD) are a direct consequence of modern living and contribute to the development of multisystem diseases such as cardiovascular diseases and diabetes mellitus (DM). CMD has reached epidemic proportions worldwide. A sodium pump (Na + /K + -ATPase) is found in most eukaryotic cells’ membrane and controls many essential cellular functions directly or indirectly. This ion transporter and its isoforms are important in the pathogenesis of some pathological processes, including CMD. The structure and function of Na + /K + -ATPase, its expression and distribution in tissues, and its interactions with known ligands such as cardiotonic steroids and other suspected endogenous regulators are discussed in this review. In addition, we reviewed recent literature data related to the involvement of Na + /K + -ATPase activity dysfunction in CMD, focusing on the Na + /K + -ATPase as a potential therapeutic target in CMD.
T2  - Frontiers in Endocrinology
T1  - The Na+/K+-ATPase: A potential therapeutic target in cardiometabolic diseases
VL  - 14
DO  - 10.3389/fendo.2023.1150171
ER  - 

@article{
author = "Obradović, Milan M. and Sudar-Milovanović, Emina and Gluvić, Zoran and Banjac, Katarina and Rizzo, Manfredi and Isenović, Esma R.",
year = "2023",
abstract = "Cardiometabolic diseases (CMD) are a direct consequence of modern living and contribute to the development of multisystem diseases such as cardiovascular diseases and diabetes mellitus (DM). CMD has reached epidemic proportions worldwide. A sodium pump (Na + /K + -ATPase) is found in most eukaryotic cells’ membrane and controls many essential cellular functions directly or indirectly. This ion transporter and its isoforms are important in the pathogenesis of some pathological processes, including CMD. The structure and function of Na + /K + -ATPase, its expression and distribution in tissues, and its interactions with known ligands such as cardiotonic steroids and other suspected endogenous regulators are discussed in this review. In addition, we reviewed recent literature data related to the involvement of Na + /K + -ATPase activity dysfunction in CMD, focusing on the Na + /K + -ATPase as a potential therapeutic target in CMD.",
journal = "Frontiers in Endocrinology",
title = "The Na+/K+-ATPase: A potential therapeutic target in cardiometabolic diseases",
volume = "14",
doi = "10.3389/fendo.2023.1150171"
}

Obradović, M. M., Sudar-Milovanović, E., Gluvić, Z., Banjac, K., Rizzo, M.,& Isenović, E. R.. (2023). The Na+/K+-ATPase: A potential therapeutic target in cardiometabolic diseases. in Frontiers in Endocrinology, 14.
https://doi.org/10.3389/fendo.2023.1150171

Obradović MM, Sudar-Milovanović E, Gluvić Z, Banjac K, Rizzo M, Isenović ER. The Na+/K+-ATPase: A potential therapeutic target in cardiometabolic diseases. in Frontiers in Endocrinology. 2023;14.
doi:10.3389/fendo.2023.1150171 .

Obradović, Milan M., Sudar-Milovanović, Emina, Gluvić, Zoran, Banjac, Katarina, Rizzo, Manfredi, Isenović, Esma R., "The Na+/K+-ATPase: A potential therapeutic target in cardiometabolic diseases" in Frontiers in Endocrinology, 14 (2023),
https://doi.org/10.3389/fendo.2023.1150171 . .

TY  - JOUR
AU  - Stanimirović, Julijana
AU  - Radovanović, Jelena
AU  - Banjac, Katarina
AU  - Obradović, Milan M.
AU  - Essack, Magbubah
AU  - Zafirović, Sonja
AU  - Gluvić, Zoran
AU  - Gojobori, Takashi
AU  - Isenović, Esma R.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10285
AB  - Even though type 2 diabetes mellitus (T2DM) represents a worldwide chronic health issue that affects about 462 million people, specific underlying determinants of insulin resistance (IR) and impaired insulin secretion are still unknown. There is growing evidence that chronic subclinical inflammation is a triggering factor in the origin of T2DM. Increased C-reactive protein (CRP) levels have been linked to excess body weight since adipocytes produce tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6), which are pivotal factors for CRP stimulation. Furthermore, it is known that hepatocytes produce relatively low rates of CRP in physiological conditions compared to T2DM patients, in which elevated levels of inflammatory markers are reported, including CRP. CRP also participates in endothelial dysfunction, the production of vasodilators, and vascular remodeling, and increased CRP level is closely associated with vascular system pathology and metabolic syndrome. In addition, insulin-based therapies may alter CRP levels in T2DM. Therefore, determining and clarifying the underlying CRP mechanism of T2DM is imperative for novel preventive and diagnostic procedures. Overall, CRP is one of the possible targets for T2DM progression and understanding the connection between insulin and inflammation may be helpful in clinical treatment and prevention approaches.
T2  - Mediators of Inflammation
T1  - Role of C-Reactive Protein in Diabetic Inflammation
VL  - 2022
SP  - e3706508
DO  - 10.1155/2022/3706508
ER  - 

@article{
author = "Stanimirović, Julijana and Radovanović, Jelena and Banjac, Katarina and Obradović, Milan M. and Essack, Magbubah and Zafirović, Sonja and Gluvić, Zoran and Gojobori, Takashi and Isenović, Esma R.",
year = "2022",
abstract = "Even though type 2 diabetes mellitus (T2DM) represents a worldwide chronic health issue that affects about 462 million people, specific underlying determinants of insulin resistance (IR) and impaired insulin secretion are still unknown. There is growing evidence that chronic subclinical inflammation is a triggering factor in the origin of T2DM. Increased C-reactive protein (CRP) levels have been linked to excess body weight since adipocytes produce tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6), which are pivotal factors for CRP stimulation. Furthermore, it is known that hepatocytes produce relatively low rates of CRP in physiological conditions compared to T2DM patients, in which elevated levels of inflammatory markers are reported, including CRP. CRP also participates in endothelial dysfunction, the production of vasodilators, and vascular remodeling, and increased CRP level is closely associated with vascular system pathology and metabolic syndrome. In addition, insulin-based therapies may alter CRP levels in T2DM. Therefore, determining and clarifying the underlying CRP mechanism of T2DM is imperative for novel preventive and diagnostic procedures. Overall, CRP is one of the possible targets for T2DM progression and understanding the connection between insulin and inflammation may be helpful in clinical treatment and prevention approaches.",
journal = "Mediators of Inflammation",
title = "Role of C-Reactive Protein in Diabetic Inflammation",
volume = "2022",
pages = "e3706508",
doi = "10.1155/2022/3706508"
}

Stanimirović, J., Radovanović, J., Banjac, K., Obradović, M. M., Essack, M., Zafirović, S., Gluvić, Z., Gojobori, T.,& Isenović, E. R.. (2022). Role of C-Reactive Protein in Diabetic Inflammation. in Mediators of Inflammation, 2022, e3706508.
https://doi.org/10.1155/2022/3706508

Stanimirović J, Radovanović J, Banjac K, Obradović MM, Essack M, Zafirović S, Gluvić Z, Gojobori T, Isenović ER. Role of C-Reactive Protein in Diabetic Inflammation. in Mediators of Inflammation. 2022;2022:e3706508.
doi:10.1155/2022/3706508 .

Stanimirović, Julijana, Radovanović, Jelena, Banjac, Katarina, Obradović, Milan M., Essack, Magbubah, Zafirović, Sonja, Gluvić, Zoran, Gojobori, Takashi, Isenović, Esma R., "Role of C-Reactive Protein in Diabetic Inflammation" in Mediators of Inflammation, 2022 (2022):e3706508,
https://doi.org/10.1155/2022/3706508 . .

TY  - JOUR
AU  - Samardžić, Vladimir
AU  - Banjac, Katarina
AU  - Obradović, Milan
AU  - Gluvić, Zoran
AU  - Isenović, Esma R.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9697
AB  - Thyroid nodules are among highly prevalent thyroid diseases. To make a distinction between benign and malignant thyroid nodules are of cumbersome significance for each endocrinologist. There is no unique and completely accurate diagnostic test, method, or even biomarker that points to a malignant thyroid nodule. Many studies in modern thyroidology are conducted to determine the usefulness of individual biomarkers, which could help clinicians detect thyroid nodules' potential malignant nature. One interesting biomarker with a promising diagnostic potential for the thyroid gland pathological conditions is nitric oxide (NO). Inducible nitric oxide synthase expression is increased in thyroiditis cases and even more in thyroid carcinoma cases, directly connected with increased NO levels in both pathological conditions. We hypothesize that the basal levels of nitrite/nitrate in serum and biopsy washout could indicate nodules' malignant nature.
T2  - Medical Hypotheses
T1  - Could the level of nitrite/nitrate contribute to malignant thyroid nodule diagnostics?
VL  - 150
SP  - 110569
DO  - 10.1016/j.mehy.2021.110569
ER  - 

@article{
author = "Samardžić, Vladimir and Banjac, Katarina and Obradović, Milan and Gluvić, Zoran and Isenović, Esma R.",
year = "2021",
abstract = "Thyroid nodules are among highly prevalent thyroid diseases. To make a distinction between benign and malignant thyroid nodules are of cumbersome significance for each endocrinologist. There is no unique and completely accurate diagnostic test, method, or even biomarker that points to a malignant thyroid nodule. Many studies in modern thyroidology are conducted to determine the usefulness of individual biomarkers, which could help clinicians detect thyroid nodules' potential malignant nature. One interesting biomarker with a promising diagnostic potential for the thyroid gland pathological conditions is nitric oxide (NO). Inducible nitric oxide synthase expression is increased in thyroiditis cases and even more in thyroid carcinoma cases, directly connected with increased NO levels in both pathological conditions. We hypothesize that the basal levels of nitrite/nitrate in serum and biopsy washout could indicate nodules' malignant nature.",
journal = "Medical Hypotheses",
title = "Could the level of nitrite/nitrate contribute to malignant thyroid nodule diagnostics?",
volume = "150",
pages = "110569",
doi = "10.1016/j.mehy.2021.110569"
}

Samardžić, V., Banjac, K., Obradović, M., Gluvić, Z.,& Isenović, E. R.. (2021). Could the level of nitrite/nitrate contribute to malignant thyroid nodule diagnostics?. in Medical Hypotheses, 150, 110569.
https://doi.org/10.1016/j.mehy.2021.110569

Samardžić V, Banjac K, Obradović M, Gluvić Z, Isenović ER. Could the level of nitrite/nitrate contribute to malignant thyroid nodule diagnostics?. in Medical Hypotheses. 2021;150:110569.
doi:10.1016/j.mehy.2021.110569 .

Samardžić, Vladimir, Banjac, Katarina, Obradović, Milan, Gluvić, Zoran, Isenović, Esma R., "Could the level of nitrite/nitrate contribute to malignant thyroid nodule diagnostics?" in Medical Hypotheses, 150 (2021):110569,
https://doi.org/10.1016/j.mehy.2021.110569 . .

TY  - JOUR
AU  - Radovanović, Jelena
AU  - Banjac, Katarina
AU  - Obradović, Milan M.
AU  - Isenović, Esma R.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10263
AB  - Reactive oxygen species (ROS) and reactive nitrogen species (RNS) play a fundamental role in regulating endothelial function and vascular tone in the physiological conditions of a vascular system. However, oxidative stress has detrimental effects on human health, and numerous studies confirmed that high ROS/RNS production contributes to the initiation and progression of cardiovascular diseases. The antioxidant defense has an essential role in the homeostatic functioning of the vascular endothelial system. Endogenous antioxidative defense includes various molecules and enzymes such as superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase.
T2  - Exploration of Medicine
T1  - Antioxidant enzymes and vascular diseases
VL  - 2
IS  - 6
SP  - 544
EP  - 555
DO  - 10.37349/emed.2021.00070
ER  - 

@article{
author = "Radovanović, Jelena and Banjac, Katarina and Obradović, Milan M. and Isenović, Esma R.",
year = "2021",
abstract = "Reactive oxygen species (ROS) and reactive nitrogen species (RNS) play a fundamental role in regulating endothelial function and vascular tone in the physiological conditions of a vascular system. However, oxidative stress has detrimental effects on human health, and numerous studies confirmed that high ROS/RNS production contributes to the initiation and progression of cardiovascular diseases. The antioxidant defense has an essential role in the homeostatic functioning of the vascular endothelial system. Endogenous antioxidative defense includes various molecules and enzymes such as superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase.",
journal = "Exploration of Medicine",
title = "Antioxidant enzymes and vascular diseases",
volume = "2",
number = "6",
pages = "544-555",
doi = "10.37349/emed.2021.00070"
}

Radovanović, J., Banjac, K., Obradović, M. M.,& Isenović, E. R.. (2021). Antioxidant enzymes and vascular diseases. in Exploration of Medicine, 2(6), 544-555.
https://doi.org/10.37349/emed.2021.00070

Radovanović J, Banjac K, Obradović MM, Isenović ER. Antioxidant enzymes and vascular diseases. in Exploration of Medicine. 2021;2(6):544-555.
doi:10.37349/emed.2021.00070 .

Radovanović, Jelena, Banjac, Katarina, Obradović, Milan M., Isenović, Esma R., "Antioxidant enzymes and vascular diseases" in Exploration of Medicine, 2, no. 6 (2021):544-555,
https://doi.org/10.37349/emed.2021.00070 . .

TY  - CHAP
AU  - Obradović, Milan
AU  - Radovanović, Jelena
AU  - Banjac, Katarina
AU  - Gluvić, Zoran
AU  - Zarić, Božidarka
AU  - Isenović, Esma R.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12031
AB  - This ten-chapter volume presents some recent advancements in health and disease. Chapter One explains the increased mortality rate owing to atherothrombosis and cardiovascular diseases. Chapter Two reviews the evidence for all current management strategies for recurrent hypoglycaemia in diabetes. Chapter Three provides information on the relationship between obesity and circulating levels of C-reactive protein. Chapter Four describes high-sensitivity C-reactive protein as a biomarker of diabetes and hypertension. Chapter Five studies the combination of two self-associating biopolymers such as high-molar-mass hyaluronan and chitosan for treatment of skin wounds. Chapter Six outlines an integrated approach to address the issues of high levels of stress and burnout in the workplace. Chapter Seven examines pericoronary adipose tissue as a possible storage and supply site for lipoproteins and apolipoproteins in the human coronary artery. Chapter Eight deals with corneal collagen cross-linking protocols for the management of keratoconus. Chapter Nine discusses free radicals, their formation and catabolism, and their beneficial and adverse effects on cellular activities. Finally, Chapter Ten details various diagnostic tests for vasculitis
PB  - Nova Science Publishers
T2  - Advances in Health and Disease
T1  - The Link between CRP and Obesity: Evidence from Human and Animal Studies
VL  - 50
SP  - 51
EP  - 73
DO  - 10.52305/INDG5982
ER  - 

@inbook{
author = "Obradović, Milan and Radovanović, Jelena and Banjac, Katarina and Gluvić, Zoran and Zarić, Božidarka and Isenović, Esma R.",
year = "2020",
abstract = "This ten-chapter volume presents some recent advancements in health and disease. Chapter One explains the increased mortality rate owing to atherothrombosis and cardiovascular diseases. Chapter Two reviews the evidence for all current management strategies for recurrent hypoglycaemia in diabetes. Chapter Three provides information on the relationship between obesity and circulating levels of C-reactive protein. Chapter Four describes high-sensitivity C-reactive protein as a biomarker of diabetes and hypertension. Chapter Five studies the combination of two self-associating biopolymers such as high-molar-mass hyaluronan and chitosan for treatment of skin wounds. Chapter Six outlines an integrated approach to address the issues of high levels of stress and burnout in the workplace. Chapter Seven examines pericoronary adipose tissue as a possible storage and supply site for lipoproteins and apolipoproteins in the human coronary artery. Chapter Eight deals with corneal collagen cross-linking protocols for the management of keratoconus. Chapter Nine discusses free radicals, their formation and catabolism, and their beneficial and adverse effects on cellular activities. Finally, Chapter Ten details various diagnostic tests for vasculitis",
publisher = "Nova Science Publishers",
journal = "Advances in Health and Disease",
booktitle = "The Link between CRP and Obesity: Evidence from Human and Animal Studies",
volume = "50",
pages = "51-73",
doi = "10.52305/INDG5982"
}

Obradović, M., Radovanović, J., Banjac, K., Gluvić, Z., Zarić, B.,& Isenović, E. R.. (2020). The Link between CRP and Obesity: Evidence from Human and Animal Studies. in Advances in Health and Disease
Nova Science Publishers., 50, 51-73.
https://doi.org/10.52305/INDG5982

Obradović M, Radovanović J, Banjac K, Gluvić Z, Zarić B, Isenović ER. The Link between CRP and Obesity: Evidence from Human and Animal Studies. in Advances in Health and Disease. 2020;50:51-73.
doi:10.52305/INDG5982 .

Obradović, Milan, Radovanović, Jelena, Banjac, Katarina, Gluvić, Zoran, Zarić, Božidarka, Isenović, Esma R., "The Link between CRP and Obesity: Evidence from Human and Animal Studies" in Advances in Health and Disease, 50 (2020):51-73,
https://doi.org/10.52305/INDG5982 . .