Scepanovic, Vuk

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Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver

Stojanovic, Marija; Scepanovic, Ljiljana; Bosnic, Olivera; Mitrovic, Dusan; Jozanov-Stankov, Olga; Scepanovic, Vuk; Scepanovic, Radomir; Stojanovic, Teja; Ilic, Slobodan; Đurić, Dragan M.

(2016)

TY  - JOUR
AU  - Stojanovic, Marija
AU  - Scepanovic, Ljiljana
AU  - Bosnic, Olivera
AU  - Mitrovic, Dusan
AU  - Jozanov-Stankov, Olga
AU  - Scepanovic, Vuk
AU  - Scepanovic, Radomir
AU  - Stojanovic, Teja
AU  - Ilic, Slobodan
AU  - Đurić, Dragan M.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1117
AB  - Oxidative stress appears to play a role in the pathogenesis of several inflammatory gastrointestinal diseases. Increased homocysteine levels may play a role in the pathogenesis of Chrons disease and ulcerative colitis. The aim of this study was to examine the influence of homocysteine on the antioxidant status of rat intestine and liver. The levels of thiobarbituric acid reactive substances (TBARS), activity of catalase (CAT) and total antioxidant status (TAS) were investigated in the isolated gut and liver of young male rats in the control group (8 rats) and after 3-hour incubation in high doses of D, L-homocysteine thionolactone (Hcy) (10 mu mol/L) (8 rats). Samples of duodenum, ileum, colon and liver were homogenized in sodium phosphate buffer (1: 10). Homogenates were centrifuged at 10000 for 10 min at 4 degrees C and the supernatant was taken for biochemical assays. Our results showed that high D, L-homocysteine thionolactone concentration reduced enzymatic catalase activity in homogenates of the isolated segments of duodenum (27.04%) p LT 0.01; ileum (37.27%), colon (34.17%) and liver (67.46%) p LT 0.001. Exposition to high D, L-homocysteine thiolactone concentration significantly increased TBARS levels in the duodenum (106.05%), ileum (47.24%), colon (112.75%) and liver (32.07%) (p LT 0.01). Homocysteine also modified the total antioxidant status of homogenates from the duodenum, ileum, colon and liver, increasing by 20.68% (duodenum), 24.74% (ileum), 14.88% (colon) and 19.35% (liver) (p LT 0.001). Homocysteine induced a consistent oxidative stress in rats intestine and liver (reduced activity of catalase and increased level of TBARS), but the elevated activity of TAS in our experiments could be explained as an adaptive response to the generated free radicals which indicates the failure of the total antioxidant defense mechanism to protect the tissues from damage caused by homocysteine.
T2  - Acta Veterinaria, Beograd
T1  - Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver
VL  - 66
IS  - 1
SP  - 26
EP  - 36
DO  - 10.1515/acve-2016-0002
ER  - 
@article{
author = "Stojanovic, Marija and Scepanovic, Ljiljana and Bosnic, Olivera and Mitrovic, Dusan and Jozanov-Stankov, Olga and Scepanovic, Vuk and Scepanovic, Radomir and Stojanovic, Teja and Ilic, Slobodan and Đurić, Dragan M.",
year = "2016",
abstract = "Oxidative stress appears to play a role in the pathogenesis of several inflammatory gastrointestinal diseases. Increased homocysteine levels may play a role in the pathogenesis of Chrons disease and ulcerative colitis. The aim of this study was to examine the influence of homocysteine on the antioxidant status of rat intestine and liver. The levels of thiobarbituric acid reactive substances (TBARS), activity of catalase (CAT) and total antioxidant status (TAS) were investigated in the isolated gut and liver of young male rats in the control group (8 rats) and after 3-hour incubation in high doses of D, L-homocysteine thionolactone (Hcy) (10 mu mol/L) (8 rats). Samples of duodenum, ileum, colon and liver were homogenized in sodium phosphate buffer (1: 10). Homogenates were centrifuged at 10000 for 10 min at 4 degrees C and the supernatant was taken for biochemical assays. Our results showed that high D, L-homocysteine thionolactone concentration reduced enzymatic catalase activity in homogenates of the isolated segments of duodenum (27.04%) p LT 0.01; ileum (37.27%), colon (34.17%) and liver (67.46%) p LT 0.001. Exposition to high D, L-homocysteine thiolactone concentration significantly increased TBARS levels in the duodenum (106.05%), ileum (47.24%), colon (112.75%) and liver (32.07%) (p LT 0.01). Homocysteine also modified the total antioxidant status of homogenates from the duodenum, ileum, colon and liver, increasing by 20.68% (duodenum), 24.74% (ileum), 14.88% (colon) and 19.35% (liver) (p LT 0.001). Homocysteine induced a consistent oxidative stress in rats intestine and liver (reduced activity of catalase and increased level of TBARS), but the elevated activity of TAS in our experiments could be explained as an adaptive response to the generated free radicals which indicates the failure of the total antioxidant defense mechanism to protect the tissues from damage caused by homocysteine.",
journal = "Acta Veterinaria, Beograd",
title = "Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver",
volume = "66",
number = "1",
pages = "26-36",
doi = "10.1515/acve-2016-0002"
}
Stojanovic, M., Scepanovic, L., Bosnic, O., Mitrovic, D., Jozanov-Stankov, O., Scepanovic, V., Scepanovic, R., Stojanovic, T., Ilic, S.,& Đurić, D. M.. (2016). Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver. in Acta Veterinaria, Beograd, 66(1), 26-36.
https://doi.org/10.1515/acve-2016-0002
Stojanovic M, Scepanovic L, Bosnic O, Mitrovic D, Jozanov-Stankov O, Scepanovic V, Scepanovic R, Stojanovic T, Ilic S, Đurić DM. Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver. in Acta Veterinaria, Beograd. 2016;66(1):26-36.
doi:10.1515/acve-2016-0002 .
Stojanovic, Marija, Scepanovic, Ljiljana, Bosnic, Olivera, Mitrovic, Dusan, Jozanov-Stankov, Olga, Scepanovic, Vuk, Scepanovic, Radomir, Stojanovic, Teja, Ilic, Slobodan, Đurić, Dragan M., "Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver" in Acta Veterinaria, Beograd, 66, no. 1 (2016):26-36,
https://doi.org/10.1515/acve-2016-0002 . .
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