TY  - JOUR
AU  - Ranjani, Singaraj
AU  - Kowshik, Jaganathan
AU  - Sophia, Josephraj
AU  - Nivetha, Ramesh
AU  - Baba, Abdul B
AU  - Veeravarmal, Veeran
AU  - Joksić, Gordana
AU  - Rutqvist, Lars E
AU  - Nilsson, Robert
AU  - Nagini, Siddavaram
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8943
AB  - Background and Objectives: The present study was undertaken to ascertain whether the modulatory effects of blueberries on cell proliferation induced by Swedish snus in the rat forestomach epithelium is mediated via abrogation of the PI3K/Akt/NFκB signaling axis that regulates cell fate decision. Methods: The transcript and protein expression of genes involved in cell cycle progression and apoptosis, as well as canonical PI3K/Akt/NF-κB signaling pathways, were analyzed by qRT-PCR, immunoblotting and ELISA. Expression profiling of noncoding RNAs (ncRNAs) that influence PI3K/Akt/NF-κB signaling was undertaken. TUNEL assay was performed using flow cytometry. Results: Administration of snus induced basal cell hyperplasia in the rat forestomach with increased cell proliferation and inhibition of apoptosis. This was associated with the activation of PI3K/Akt/NFκB signaling. Coadministration of blueberries significantly suppressed snus-induced hyperplasia. Analysis of the molecular mechanisms revealed that blueberries suppress the phosphorylation of Akt, NF-κB and IKKβ, prevent nuclear translocation of NF-κB and modulate the expression of microRNAs that influence PI3K/Akt/NF-κB signaling. Conclusion: Taken together, the results of the current study provide compelling evidence that blueberries exert significant protective effects against snus-induced soft tissue changes in the rat forestomach epithelium mediated by inhibiting key molecular players in the PI3K/Akt/NF-κB signaling axis. Long-term studies on the impact of snus exposure on various cellular processes, signaling pathways, and the interplay between genetic and epigenetic mechanisms are however warranted. The results of this investigation may contribute to the development of protection against soft tissue changes induced by smokeless tobacco in the human oral cavity.
T2  - Anti-Cancer Agents in Medicinal Chemistry
T1  - Activation of PI3K/Akt/NF-kB Signaling Mediates Swedish Snus Induced Proliferation and Apoptosis Evasion in the Rat Forestomach: Modulation by Blueberry
VL  - 20
IS  - 1
SP  - 59
EP  - 69
DO  - 10.2174/1871520619666191024115738
ER  - 

@article{
author = "Ranjani, Singaraj and Kowshik, Jaganathan and Sophia, Josephraj and Nivetha, Ramesh and Baba, Abdul B and Veeravarmal, Veeran and Joksić, Gordana and Rutqvist, Lars E and Nilsson, Robert and Nagini, Siddavaram",
year = "2020",
abstract = "Background and Objectives: The present study was undertaken to ascertain whether the modulatory effects of blueberries on cell proliferation induced by Swedish snus in the rat forestomach epithelium is mediated via abrogation of the PI3K/Akt/NFκB signaling axis that regulates cell fate decision. Methods: The transcript and protein expression of genes involved in cell cycle progression and apoptosis, as well as canonical PI3K/Akt/NF-κB signaling pathways, were analyzed by qRT-PCR, immunoblotting and ELISA. Expression profiling of noncoding RNAs (ncRNAs) that influence PI3K/Akt/NF-κB signaling was undertaken. TUNEL assay was performed using flow cytometry. Results: Administration of snus induced basal cell hyperplasia in the rat forestomach with increased cell proliferation and inhibition of apoptosis. This was associated with the activation of PI3K/Akt/NFκB signaling. Coadministration of blueberries significantly suppressed snus-induced hyperplasia. Analysis of the molecular mechanisms revealed that blueberries suppress the phosphorylation of Akt, NF-κB and IKKβ, prevent nuclear translocation of NF-κB and modulate the expression of microRNAs that influence PI3K/Akt/NF-κB signaling. Conclusion: Taken together, the results of the current study provide compelling evidence that blueberries exert significant protective effects against snus-induced soft tissue changes in the rat forestomach epithelium mediated by inhibiting key molecular players in the PI3K/Akt/NF-κB signaling axis. Long-term studies on the impact of snus exposure on various cellular processes, signaling pathways, and the interplay between genetic and epigenetic mechanisms are however warranted. The results of this investigation may contribute to the development of protection against soft tissue changes induced by smokeless tobacco in the human oral cavity.",
journal = "Anti-Cancer Agents in Medicinal Chemistry",
title = "Activation of PI3K/Akt/NF-kB Signaling Mediates Swedish Snus Induced Proliferation and Apoptosis Evasion in the Rat Forestomach: Modulation by Blueberry",
volume = "20",
number = "1",
pages = "59-69",
doi = "10.2174/1871520619666191024115738"
}

Ranjani, S., Kowshik, J., Sophia, J., Nivetha, R., Baba, A. B., Veeravarmal, V., Joksić, G., Rutqvist, L. E., Nilsson, R.,& Nagini, S.. (2020). Activation of PI3K/Akt/NF-kB Signaling Mediates Swedish Snus Induced Proliferation and Apoptosis Evasion in the Rat Forestomach: Modulation by Blueberry. in Anti-Cancer Agents in Medicinal Chemistry, 20(1), 59-69.
https://doi.org/10.2174/1871520619666191024115738

Ranjani S, Kowshik J, Sophia J, Nivetha R, Baba AB, Veeravarmal V, Joksić G, Rutqvist LE, Nilsson R, Nagini S. Activation of PI3K/Akt/NF-kB Signaling Mediates Swedish Snus Induced Proliferation and Apoptosis Evasion in the Rat Forestomach: Modulation by Blueberry. in Anti-Cancer Agents in Medicinal Chemistry. 2020;20(1):59-69.
doi:10.2174/1871520619666191024115738 .

Ranjani, Singaraj, Kowshik, Jaganathan, Sophia, Josephraj, Nivetha, Ramesh, Baba, Abdul B, Veeravarmal, Veeran, Joksić, Gordana, Rutqvist, Lars E, Nilsson, Robert, Nagini, Siddavaram, "Activation of PI3K/Akt/NF-kB Signaling Mediates Swedish Snus Induced Proliferation and Apoptosis Evasion in the Rat Forestomach: Modulation by Blueberry" in Anti-Cancer Agents in Medicinal Chemistry, 20, no. 1 (2020):59-69,
https://doi.org/10.2174/1871520619666191024115738 . .