Milanović, Zorana

Link to this page

Authority KeyName Variants
orcid::0000-0001-9953-3269
  • Milanović, Zorana (15)
Projects
EUREKA [E! 13303 MED-BIO-TEST] Magnetic and radionuclide labeled nanostructured materials for medical applications
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200146 (University of Belgrade, Faculty of Physical Chemistry)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200168 (University of Belgrade, Faculty of Chemistry) COST Action RADIOMAG [TD1402]
Grant Agency of the Slovak Republic VEGA [1/0159/20] Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials
Rational design and synthesis of biologically active and coordination compounds and functional materials, relevant for (bio)nanotechnology Antioxidative defense, differentiation and regeneration potential of tissue specific mesenchymal stem cells during ageing
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200288 (Innovation Center of the Faculty of Chemistry) Studying climate change and its influence on environment: impacts, adaptation and mitigation
Advanced technologies for monitoring and environmental protection from chemical pollutants and radiation burden Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije (451-03-68/2020-14)
Ministry of Education, Science and Technological Development of the Republic of Serbia The VINCENT Center of Excellence.

Author's Bibliography

Co(III), Ni(II) and Cu(II) complexes with a tetradentate Schiff base ligand: synthesis, characterization, electrochemical behavior, binding assessment and in vitro cytotoxicity

Mirković, Marija; Radović, Magdalena; Stanković, Dalibor M.; Vranješ-Đurić, Sanja; Janković, Drina; Petrović, Djordje; Mihajlović-Lalić, Ljiljana E.; Prijović, Željko; Milanović, Zorana

(2022)

TY  - JOUR
AU  - Mirković, Marija
AU  - Radović, Magdalena
AU  - Stanković, Dalibor M.
AU  - Vranješ-Đurić, Sanja
AU  - Janković, Drina
AU  - Petrović, Djordje
AU  - Mihajlović-Lalić, Ljiljana E.
AU  - Prijović, Željko
AU  - Milanović, Zorana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10169
AB  - Two new Schiff base cobalt(III) ([Co(LH)Cl2], 1) and nickel(II) ([Ni(LH)ClO4], 2) complexes with a diimine-dioxime ligand, (4,9-diaza-3,10-diethyl-3,9-dodecadiene-2,11-dione bis oxime (LH2)), were synthesized and characterized. The compounds were obtained in MeOH from corresponding metal salts and LH2 in molar ratio 1:1 and further characterized by mass spectrometry, IR spectroscopy, electrochemistry, and elemental analysis. Previously reported copper(II) analog ([Cu2(LH)2]·(ClO4)2, 3) was joined to 1 and 2, and the three metal analogs, 1–3, were further investigated in terms of their electrochemical behavior. Binding studies of the complexes with deoxyribonucleic acid (DNA) and human serum albumin (HSA) were carried out using both spectrophotometric and electrochemical methods. All three complexes exhibit binding affinity towards the DNA chain through intercalative interactions. The binding reaction with HSA showed for 1 and 3 decrease in the peak current obtained in the case of complexes before the addition of HSA, while the Ni complex–HSA possesses the same electroactivity as starting complex. The cytotoxicity of LH2 as well as its metal complexes, and cisplatin were evaluated on CT-26 mouse colon carcinoma and human LS174T cancer cell lines employing MTT assay. The copper(II) complex exhibited very promising anticancer activity compared with cisplatin.
T2  - Journal of Coordination Chemistry
T1  - Co(III), Ni(II) and Cu(II) complexes with a tetradentate Schiff base ligand: synthesis, characterization, electrochemical behavior, binding assessment and in vitro cytotoxicity
SP  - 1
EP  - 14
DO  - 10.1080/00958972.2022.2032683
ER  - 
@article{
author = "Mirković, Marija and Radović, Magdalena and Stanković, Dalibor M. and Vranješ-Đurić, Sanja and Janković, Drina and Petrović, Djordje and Mihajlović-Lalić, Ljiljana E. and Prijović, Željko and Milanović, Zorana",
year = "2022",
abstract = "Two new Schiff base cobalt(III) ([Co(LH)Cl2], 1) and nickel(II) ([Ni(LH)ClO4], 2) complexes with a diimine-dioxime ligand, (4,9-diaza-3,10-diethyl-3,9-dodecadiene-2,11-dione bis oxime (LH2)), were synthesized and characterized. The compounds were obtained in MeOH from corresponding metal salts and LH2 in molar ratio 1:1 and further characterized by mass spectrometry, IR spectroscopy, electrochemistry, and elemental analysis. Previously reported copper(II) analog ([Cu2(LH)2]·(ClO4)2, 3) was joined to 1 and 2, and the three metal analogs, 1–3, were further investigated in terms of their electrochemical behavior. Binding studies of the complexes with deoxyribonucleic acid (DNA) and human serum albumin (HSA) were carried out using both spectrophotometric and electrochemical methods. All three complexes exhibit binding affinity towards the DNA chain through intercalative interactions. The binding reaction with HSA showed for 1 and 3 decrease in the peak current obtained in the case of complexes before the addition of HSA, while the Ni complex–HSA possesses the same electroactivity as starting complex. The cytotoxicity of LH2 as well as its metal complexes, and cisplatin were evaluated on CT-26 mouse colon carcinoma and human LS174T cancer cell lines employing MTT assay. The copper(II) complex exhibited very promising anticancer activity compared with cisplatin.",
journal = "Journal of Coordination Chemistry",
title = "Co(III), Ni(II) and Cu(II) complexes with a tetradentate Schiff base ligand: synthesis, characterization, electrochemical behavior, binding assessment and in vitro cytotoxicity",
pages = "1-14",
doi = "10.1080/00958972.2022.2032683"
}
Mirković, M., Radović, M., Stanković, D. M., Vranješ-Đurić, S., Janković, D., Petrović, D., Mihajlović-Lalić, L. E., Prijović, Ž.,& Milanović, Z.. (2022). Co(III), Ni(II) and Cu(II) complexes with a tetradentate Schiff base ligand: synthesis, characterization, electrochemical behavior, binding assessment and in vitro cytotoxicity. in Journal of Coordination Chemistry, 1-14.
https://doi.org/10.1080/00958972.2022.2032683
Mirković M, Radović M, Stanković DM, Vranješ-Đurić S, Janković D, Petrović D, Mihajlović-Lalić LE, Prijović Ž, Milanović Z. Co(III), Ni(II) and Cu(II) complexes with a tetradentate Schiff base ligand: synthesis, characterization, electrochemical behavior, binding assessment and in vitro cytotoxicity. in Journal of Coordination Chemistry. 2022;:1-14.
doi:10.1080/00958972.2022.2032683 .
Mirković, Marija, Radović, Magdalena, Stanković, Dalibor M., Vranješ-Đurić, Sanja, Janković, Drina, Petrović, Djordje, Mihajlović-Lalić, Ljiljana E., Prijović, Željko, Milanović, Zorana, "Co(III), Ni(II) and Cu(II) complexes with a tetradentate Schiff base ligand: synthesis, characterization, electrochemical behavior, binding assessment and in vitro cytotoxicity" in Journal of Coordination Chemistry (2022):1-14,
https://doi.org/10.1080/00958972.2022.2032683 . .
1

177Lu–labeled micro liposomes as a potential radiosynoviorthesis therapeutic agent

Cvjetinović, Đorđe; Janković, Drina; Milanović, Zorana; Mirković, Marija D.; Petrović, Jelena; Prijović, Željko; Poghosyan, Emiliya; Vranješ-Đurić, Sanja

(2021)

TY  - JOUR
AU  - Cvjetinović, Đorđe
AU  - Janković, Drina
AU  - Milanović, Zorana
AU  - Mirković, Marija D.
AU  - Petrović, Jelena
AU  - Prijović, Željko
AU  - Poghosyan, Emiliya
AU  - Vranješ-Đurić, Sanja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9946
AB  - Micro–sized multivesicular liposomes were prepared, radiolabeled with 177Lu, and tested in vitro and in vivo to evaluate the potential of 177Lu–labeled micro liposomes in radiosynoviorthesis (RSO) therapy. A standard reverse–phase procedure of liposome preparation with a lipid mixture of DPPC: CHOL (80:20%) was used for the synthesis. TEM and fluorescence microscopy imaging were performed to determine the size, shape, and structure of the prepared liposomes. Both measurements are in good agreement while TEM micrographs additionally indicate to a large multivesicular inner structure of prepared liposomes. A simple and straightforward procedure was used for liposome radiolabeling with 177Lu, a well–known and commonly used radionuclide in radiotherapy with favorable properties, that can be exploited in RSO therapy. Radiolabeled 177Lu–liposomes were tested in vitro for stability and then injected into the knee joints of Wistar rats where liposome in vivo behavior was followed up to 30 days post injection. Results from both ex vivo biodistribution and in vivo imaging studies presented a high stability and retention (>94 %ID) of 177Lu–micro liposomes in the synovial liquid for the entire observation period. Leakage of free 177Lu or 177Lu–liposomes from the synovial fluid has not been detected, indicating to a possible application of 177Lu–liposomes in radiosynoviorthesis (RSO) therapy.
T2  - International Journal of Pharmaceutics
T2  - International Journal of PharmaceuticsInternational Journal of Pharmaceutics
T1  - 177Lu–labeled micro liposomes as a potential radiosynoviorthesis therapeutic agent
VL  - 608
SP  - 121106
DO  - 10.1016/j.ijpharm.2021.121106
ER  - 
@article{
author = "Cvjetinović, Đorđe and Janković, Drina and Milanović, Zorana and Mirković, Marija D. and Petrović, Jelena and Prijović, Željko and Poghosyan, Emiliya and Vranješ-Đurić, Sanja",
year = "2021",
abstract = "Micro–sized multivesicular liposomes were prepared, radiolabeled with 177Lu, and tested in vitro and in vivo to evaluate the potential of 177Lu–labeled micro liposomes in radiosynoviorthesis (RSO) therapy. A standard reverse–phase procedure of liposome preparation with a lipid mixture of DPPC: CHOL (80:20%) was used for the synthesis. TEM and fluorescence microscopy imaging were performed to determine the size, shape, and structure of the prepared liposomes. Both measurements are in good agreement while TEM micrographs additionally indicate to a large multivesicular inner structure of prepared liposomes. A simple and straightforward procedure was used for liposome radiolabeling with 177Lu, a well–known and commonly used radionuclide in radiotherapy with favorable properties, that can be exploited in RSO therapy. Radiolabeled 177Lu–liposomes were tested in vitro for stability and then injected into the knee joints of Wistar rats where liposome in vivo behavior was followed up to 30 days post injection. Results from both ex vivo biodistribution and in vivo imaging studies presented a high stability and retention (>94 %ID) of 177Lu–micro liposomes in the synovial liquid for the entire observation period. Leakage of free 177Lu or 177Lu–liposomes from the synovial fluid has not been detected, indicating to a possible application of 177Lu–liposomes in radiosynoviorthesis (RSO) therapy.",
journal = "International Journal of Pharmaceutics, International Journal of PharmaceuticsInternational Journal of Pharmaceutics",
title = "177Lu–labeled micro liposomes as a potential radiosynoviorthesis therapeutic agent",
volume = "608",
pages = "121106",
doi = "10.1016/j.ijpharm.2021.121106"
}
Cvjetinović, Đ., Janković, D., Milanović, Z., Mirković, M. D., Petrović, J., Prijović, Ž., Poghosyan, E.,& Vranješ-Đurić, S.. (2021). 177Lu–labeled micro liposomes as a potential radiosynoviorthesis therapeutic agent. in International Journal of Pharmaceutics, 608, 121106.
https://doi.org/10.1016/j.ijpharm.2021.121106
Cvjetinović Đ, Janković D, Milanović Z, Mirković MD, Petrović J, Prijović Ž, Poghosyan E, Vranješ-Đurić S. 177Lu–labeled micro liposomes as a potential radiosynoviorthesis therapeutic agent. in International Journal of Pharmaceutics. 2021;608:121106.
doi:10.1016/j.ijpharm.2021.121106 .
Cvjetinović, Đorđe, Janković, Drina, Milanović, Zorana, Mirković, Marija D., Petrović, Jelena, Prijović, Željko, Poghosyan, Emiliya, Vranješ-Đurić, Sanja, "177Lu–labeled micro liposomes as a potential radiosynoviorthesis therapeutic agent" in International Journal of Pharmaceutics, 608 (2021):121106,
https://doi.org/10.1016/j.ijpharm.2021.121106 . .

Magnetically induced controlled release from glucose-modified liposomes loaded with Fe3O4 nanoparticles

Cvjetinović, Đorđe; Milanović, Zorana; Mirković, Marija D.; Petrović, Jelena; Vesković, Ana; Popović-Bijelić, Ana; Prijović, Željko; Janković, Drina; Vranješ-Đurić, Sanja

(2021)

TY  - JOUR
AU  - Cvjetinović, Đorđe
AU  - Milanović, Zorana
AU  - Mirković, Marija D.
AU  - Petrović, Jelena
AU  - Vesković, Ana
AU  - Popović-Bijelić, Ana
AU  - Prijović, Željko
AU  - Janković, Drina
AU  - Vranješ-Đurić, Sanja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10063
AB  - Small glucose-modified liposomes (GMLs) were loaded with magnetic Fe3O4 nanoparticles (MNPs) and fluorescein using a standard thin layer preparation procedure and a varying lipid/MNPs ratio. The liposomes were characterized with TEM and DLS measurements, and MNPs encapsulation rate was determined using ICP-OES. Prepared liposomes were stored at 5 °C for 30 days and subsequently exposed to an external magnetic field (20 mT) with varying exposure times (2‒20 min), at room temperature. The release of fluorescein from GMLs induced by the magnetic field exposures was quantified, showing a high release rate (25‒85%) depending on the concentration of MNPs in GMLs. EPR measurements were conducted during the liposomes storage period in order to provide semi-quantitative information of possible MNPs oxidation from Fe3O4 to Fe2O3 inside the liposomes, impacting MNPs magnetic properties. In contrast to the MNPs water dispersion, no significant change in the EPR signal of MNPs encapsulated inside GMLs was detected over the course of 30 days. The data presented in this study indicate that GMLs loaded with MNPs maintain a high stability for prolonged periods of time and that this delivery system may be used for magnetically assisted controlled drug release.
T2  - Journal of Nanoparticle Research
T1  - Magnetically induced controlled release from glucose-modified liposomes loaded with Fe3O4 nanoparticles
VL  - 23
IS  - 11
SP  - 252
DO  - 10.1007/s11051-021-05375-2
ER  - 
@article{
author = "Cvjetinović, Đorđe and Milanović, Zorana and Mirković, Marija D. and Petrović, Jelena and Vesković, Ana and Popović-Bijelić, Ana and Prijović, Željko and Janković, Drina and Vranješ-Đurić, Sanja",
year = "2021",
abstract = "Small glucose-modified liposomes (GMLs) were loaded with magnetic Fe3O4 nanoparticles (MNPs) and fluorescein using a standard thin layer preparation procedure and a varying lipid/MNPs ratio. The liposomes were characterized with TEM and DLS measurements, and MNPs encapsulation rate was determined using ICP-OES. Prepared liposomes were stored at 5 °C for 30 days and subsequently exposed to an external magnetic field (20 mT) with varying exposure times (2‒20 min), at room temperature. The release of fluorescein from GMLs induced by the magnetic field exposures was quantified, showing a high release rate (25‒85%) depending on the concentration of MNPs in GMLs. EPR measurements were conducted during the liposomes storage period in order to provide semi-quantitative information of possible MNPs oxidation from Fe3O4 to Fe2O3 inside the liposomes, impacting MNPs magnetic properties. In contrast to the MNPs water dispersion, no significant change in the EPR signal of MNPs encapsulated inside GMLs was detected over the course of 30 days. The data presented in this study indicate that GMLs loaded with MNPs maintain a high stability for prolonged periods of time and that this delivery system may be used for magnetically assisted controlled drug release.",
journal = "Journal of Nanoparticle Research",
title = "Magnetically induced controlled release from glucose-modified liposomes loaded with Fe3O4 nanoparticles",
volume = "23",
number = "11",
pages = "252",
doi = "10.1007/s11051-021-05375-2"
}
Cvjetinović, Đ., Milanović, Z., Mirković, M. D., Petrović, J., Vesković, A., Popović-Bijelić, A., Prijović, Ž., Janković, D.,& Vranješ-Đurić, S.. (2021). Magnetically induced controlled release from glucose-modified liposomes loaded with Fe3O4 nanoparticles. in Journal of Nanoparticle Research, 23(11), 252.
https://doi.org/10.1007/s11051-021-05375-2
Cvjetinović Đ, Milanović Z, Mirković MD, Petrović J, Vesković A, Popović-Bijelić A, Prijović Ž, Janković D, Vranješ-Đurić S. Magnetically induced controlled release from glucose-modified liposomes loaded with Fe3O4 nanoparticles. in Journal of Nanoparticle Research. 2021;23(11):252.
doi:10.1007/s11051-021-05375-2 .
Cvjetinović, Đorđe, Milanović, Zorana, Mirković, Marija D., Petrović, Jelena, Vesković, Ana, Popović-Bijelić, Ana, Prijović, Željko, Janković, Drina, Vranješ-Đurić, Sanja, "Magnetically induced controlled release from glucose-modified liposomes loaded with Fe3O4 nanoparticles" in Journal of Nanoparticle Research, 23, no. 11 (2021):252,
https://doi.org/10.1007/s11051-021-05375-2 . .

Transmittance Measurements in Non-alternating Magnetic Field as Reliable Method for Determining of Heating Properties of Phosphate and Phosphonate Coated Fe3O4 Magnetic Nanoparticles

Radović, Magdalena; Mirković, Marija D.; Nikolić, Aleksandar S.; Kuraica, Milorad M.; Iskrenović, Predrag; Milanović, Zorana; Vranješ-Đurić, Sanja; Perić, Marko

(2021)

TY  - JOUR
AU  - Radović, Magdalena
AU  - Mirković, Marija D.
AU  - Nikolić, Aleksandar S.
AU  - Kuraica, Milorad M.
AU  - Iskrenović, Predrag
AU  - Milanović, Zorana
AU  - Vranješ-Đurić, Sanja
AU  - Perić, Marko
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9850
AB  - Different phosphates and phosphonates have shown excellent coating ability toward magnetic nanoparticles, improving their stability and biocompatibility which enables their biomedical application. The magnetic hyperthermia efficiency of phosphates (IDP and IHP) and phosphonates (MDP and HEDP) coated Fe3O4 magnetic nanoparticles (MNPs) were evaluated in an alternating magnetic field. For a deeper understanding of hyperthermia, the behavior of investigated MNPs in the non-alternating magnetic field was monitored by measuring the transparency of the sample. To investigate their theranostic potential coated Fe3O4-MNPs were radiolabeled with radionuclide 177Lu. Phosphate coated MNPs were radiolabeled in high radiolabeling yield (> 99%) while phosphonate coated MNPs reached maximum radiolabeling yield of 78%. Regardless lower radiolabeling yield both radiolabeled phosphonate MNPs may be further purified reaching radiochemical purity of more than 95%. In vitro stabile radiolabeled nanoparticles in saline and HSA were obtained. The high heating ability of phosphates and phosphonates coated MNPs as sine qua non for efficient in vivo hyperthermia treatment and satisfactory radiolabeling yield justifies their further research in order to develop new theranostic agents. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Journal of Inorganic and Organometallic Polymers and Materials
T1  - Transmittance Measurements in Non-alternating Magnetic Field as Reliable Method for Determining of Heating Properties of Phosphate and Phosphonate Coated Fe3O4 Magnetic Nanoparticles
DO  - 10.1007/s10904-021-02059-1
ER  - 
@article{
author = "Radović, Magdalena and Mirković, Marija D. and Nikolić, Aleksandar S. and Kuraica, Milorad M. and Iskrenović, Predrag and Milanović, Zorana and Vranješ-Đurić, Sanja and Perić, Marko",
year = "2021",
abstract = "Different phosphates and phosphonates have shown excellent coating ability toward magnetic nanoparticles, improving their stability and biocompatibility which enables their biomedical application. The magnetic hyperthermia efficiency of phosphates (IDP and IHP) and phosphonates (MDP and HEDP) coated Fe3O4 magnetic nanoparticles (MNPs) were evaluated in an alternating magnetic field. For a deeper understanding of hyperthermia, the behavior of investigated MNPs in the non-alternating magnetic field was monitored by measuring the transparency of the sample. To investigate their theranostic potential coated Fe3O4-MNPs were radiolabeled with radionuclide 177Lu. Phosphate coated MNPs were radiolabeled in high radiolabeling yield (> 99%) while phosphonate coated MNPs reached maximum radiolabeling yield of 78%. Regardless lower radiolabeling yield both radiolabeled phosphonate MNPs may be further purified reaching radiochemical purity of more than 95%. In vitro stabile radiolabeled nanoparticles in saline and HSA were obtained. The high heating ability of phosphates and phosphonates coated MNPs as sine qua non for efficient in vivo hyperthermia treatment and satisfactory radiolabeling yield justifies their further research in order to develop new theranostic agents. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Journal of Inorganic and Organometallic Polymers and Materials",
title = "Transmittance Measurements in Non-alternating Magnetic Field as Reliable Method for Determining of Heating Properties of Phosphate and Phosphonate Coated Fe3O4 Magnetic Nanoparticles",
doi = "10.1007/s10904-021-02059-1"
}
Radović, M., Mirković, M. D., Nikolić, A. S., Kuraica, M. M., Iskrenović, P., Milanović, Z., Vranješ-Đurić, S.,& Perić, M.. (2021). Transmittance Measurements in Non-alternating Magnetic Field as Reliable Method for Determining of Heating Properties of Phosphate and Phosphonate Coated Fe3O4 Magnetic Nanoparticles. in Journal of Inorganic and Organometallic Polymers and Materials.
https://doi.org/10.1007/s10904-021-02059-1
Radović M, Mirković MD, Nikolić AS, Kuraica MM, Iskrenović P, Milanović Z, Vranješ-Đurić S, Perić M. Transmittance Measurements in Non-alternating Magnetic Field as Reliable Method for Determining of Heating Properties of Phosphate and Phosphonate Coated Fe3O4 Magnetic Nanoparticles. in Journal of Inorganic and Organometallic Polymers and Materials. 2021;.
doi:10.1007/s10904-021-02059-1 .
Radović, Magdalena, Mirković, Marija D., Nikolić, Aleksandar S., Kuraica, Milorad M., Iskrenović, Predrag, Milanović, Zorana, Vranješ-Đurić, Sanja, Perić, Marko, "Transmittance Measurements in Non-alternating Magnetic Field as Reliable Method for Determining of Heating Properties of Phosphate and Phosphonate Coated Fe3O4 Magnetic Nanoparticles" in Journal of Inorganic and Organometallic Polymers and Materials (2021),
https://doi.org/10.1007/s10904-021-02059-1 . .

177Lu-doxycycline as potential radiopharmaceutical: electrochemical characterization, radiolabeling, and biodistribution in tumor-bearing mice

Milanović, Zorana; Janković, Drina; Vranješ-Đurić, Sanja; Radović, Magdalena; Prijović, Željko; Zavišić, Gordana; Perić, Marko; Stanković, Dalibor M.; Mirković, Marija D.

(2021)

TY  - JOUR
AU  - Milanović, Zorana
AU  - Janković, Drina
AU  - Vranješ-Đurić, Sanja
AU  - Radović, Magdalena
AU  - Prijović, Željko
AU  - Zavišić, Gordana
AU  - Perić, Marko
AU  - Stanković, Dalibor M.
AU  - Mirković, Marija D.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9960
AB  - Purpose Recent studies with doxycycline as adjuvant therapy to conventional chemotherapy have shown promising results in cancer therapy. The current study aimed to examine the capability of 177Lu-labeled tetracycline ligand, doxycycline hyclate, to use as an anticancer agent.Materials and methods Doxycycline was radiolabeled with beta-emitting radioisotope 177Lu. Complex formation and its interaction with DNA were investigated electrochemically. Binding of 177Lu-doxycycline to CT 26 cell line was done. Biodistribution of 177Lu-doxycycline was examined in healthy Wistar rats and CT26 colon carcinoma tumor-bearing mice by i.v. and i.p. administration, respectively.Results Doxycycline hyclate was successfully radiolabeled with 177Lu in high radiolabeling yield (>99%). The radiolabeled complex was stable in vitro in saline and human serum over 72 h. Non-radioactive Lu-doxycycline complex formation was demonstrated electrochemically as well. Intercalative interactions of the doxycycline and Lu-doxycycline with DNA were proved using simultaneously spectrophotometric and electrochemical methods. The binding of the radiolabeled complex with plasma proteins was 4.0 ± 0.4%. The partition coefficient showed the lipophilic nature of the complex similar to the free ligand. The binding curve demonstrates binding from 0.1 nM concentrations of 177Lu-doxycycline, with half-binding estimated ∼100 nM. Biodistribution studies of 177Lu-doxycycline in CT26 colon tumor-bearing mice showed a satisfactory accumulation rate in the tumor (2.88 ± 0.85% ID/g) 3 h after intraperitoneal injection. Both the hepatobiliary system and the urinary system were prominent as excretory routes of the radiolabeled complex.Conclusion Considering obtained results, 177Lu-doxycycline complex, due to its excellent electrochemical and biological characteristics, with emphasis on the binding ability to DNA via intercalative interaction as well as significant accumulation in the tumor, is suitable for further in vivo studies to investigate its potential use in cancer treatment.
T2  - International Journal of Radiation Biology
T1  - 177Lu-doxycycline as potential radiopharmaceutical: electrochemical characterization, radiolabeling, and biodistribution in tumor-bearing mice
SP  - 1
EP  - 9
DO  - 10.1080/09553002.2021.1976864
ER  - 
@article{
author = "Milanović, Zorana and Janković, Drina and Vranješ-Đurić, Sanja and Radović, Magdalena and Prijović, Željko and Zavišić, Gordana and Perić, Marko and Stanković, Dalibor M. and Mirković, Marija D.",
year = "2021",
abstract = "Purpose Recent studies with doxycycline as adjuvant therapy to conventional chemotherapy have shown promising results in cancer therapy. The current study aimed to examine the capability of 177Lu-labeled tetracycline ligand, doxycycline hyclate, to use as an anticancer agent.Materials and methods Doxycycline was radiolabeled with beta-emitting radioisotope 177Lu. Complex formation and its interaction with DNA were investigated electrochemically. Binding of 177Lu-doxycycline to CT 26 cell line was done. Biodistribution of 177Lu-doxycycline was examined in healthy Wistar rats and CT26 colon carcinoma tumor-bearing mice by i.v. and i.p. administration, respectively.Results Doxycycline hyclate was successfully radiolabeled with 177Lu in high radiolabeling yield (>99%). The radiolabeled complex was stable in vitro in saline and human serum over 72 h. Non-radioactive Lu-doxycycline complex formation was demonstrated electrochemically as well. Intercalative interactions of the doxycycline and Lu-doxycycline with DNA were proved using simultaneously spectrophotometric and electrochemical methods. The binding of the radiolabeled complex with plasma proteins was 4.0 ± 0.4%. The partition coefficient showed the lipophilic nature of the complex similar to the free ligand. The binding curve demonstrates binding from 0.1 nM concentrations of 177Lu-doxycycline, with half-binding estimated ∼100 nM. Biodistribution studies of 177Lu-doxycycline in CT26 colon tumor-bearing mice showed a satisfactory accumulation rate in the tumor (2.88 ± 0.85% ID/g) 3 h after intraperitoneal injection. Both the hepatobiliary system and the urinary system were prominent as excretory routes of the radiolabeled complex.Conclusion Considering obtained results, 177Lu-doxycycline complex, due to its excellent electrochemical and biological characteristics, with emphasis on the binding ability to DNA via intercalative interaction as well as significant accumulation in the tumor, is suitable for further in vivo studies to investigate its potential use in cancer treatment.",
journal = "International Journal of Radiation Biology",
title = "177Lu-doxycycline as potential radiopharmaceutical: electrochemical characterization, radiolabeling, and biodistribution in tumor-bearing mice",
pages = "1-9",
doi = "10.1080/09553002.2021.1976864"
}
Milanović, Z., Janković, D., Vranješ-Đurić, S., Radović, M., Prijović, Ž., Zavišić, G., Perić, M., Stanković, D. M.,& Mirković, M. D.. (2021). 177Lu-doxycycline as potential radiopharmaceutical: electrochemical characterization, radiolabeling, and biodistribution in tumor-bearing mice. in International Journal of Radiation Biology, 1-9.
https://doi.org/10.1080/09553002.2021.1976864
Milanović Z, Janković D, Vranješ-Đurić S, Radović M, Prijović Ž, Zavišić G, Perić M, Stanković DM, Mirković MD. 177Lu-doxycycline as potential radiopharmaceutical: electrochemical characterization, radiolabeling, and biodistribution in tumor-bearing mice. in International Journal of Radiation Biology. 2021;:1-9.
doi:10.1080/09553002.2021.1976864 .
Milanović, Zorana, Janković, Drina, Vranješ-Đurić, Sanja, Radović, Magdalena, Prijović, Željko, Zavišić, Gordana, Perić, Marko, Stanković, Dalibor M., Mirković, Marija D., "177Lu-doxycycline as potential radiopharmaceutical: electrochemical characterization, radiolabeling, and biodistribution in tumor-bearing mice" in International Journal of Radiation Biology (2021):1-9,
https://doi.org/10.1080/09553002.2021.1976864 . .
1

Screen printed diamond electrode as efficient “point-of-care” platform for submicromolar determination of cytostatic drug in biological fluids and pharmaceutical product

Stanković, Dalibor M.; Milanović, Zorana; Švorc, Lubomir; Stanković, Vesna; Janković, Drina; Mirković, Marija D.; Vranješ-Đurić, Sanja

(2021)

TY  - JOUR
AU  - Stanković, Dalibor M.
AU  - Milanović, Zorana
AU  - Švorc, Lubomir
AU  - Stanković, Vesna
AU  - Janković, Drina
AU  - Mirković, Marija D.
AU  - Vranješ-Đurić, Sanja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9543
AB  - This work presents advanced electrochemical platform based on screen printed diamond electrode (SPDE) system for the single drop “point-of-care” testing. Proposed approach was applied for the quantification of doxorubicin hydrochloride (DOX) in biological fluids and pharmaceutical product. Using a single drop (~30 μL) of the tested solution at the electrode surface, DOX showed high electroactivity over a wide range of pHs. In these conditions, single oval shaped, well-defined and pH dependent oxidation peak was observed in the potential range from 0.5 V to 1.3 V. In the reverse scan, two cathodic peaks, were noted – around 0.3 V and – 0.5 V. Similarly, first reduction peak was pH dependent, while second one was independent in the studied range. Experimental conditions for DOX quantification were optimized and natures of the electrode reactions were investigated. Working linear range obtained for DOX detection was from 0.1 to 2.5 μM. Diffusion controlled electrode reaction reveal long life time of the proposed electrode as well high potential for practical application. Developed procedure was successfully applied for the DOX analysis in biological fluids – urine and pharmaceutical formulation. Obtained results clearly indicated that given procedure can be easily implemented for pharmaceutical control and medical analysis, in both, laboratory and field conditions. © 2021 Elsevier B.V.
T2  - Diamond and Related Materials
T1  - Screen printed diamond electrode as efficient “point-of-care” platform for submicromolar determination of cytostatic drug in biological fluids and pharmaceutical product
VL  - 113
SP  - 108277
DO  - 10.1016/j.diamond.2021.108277
ER  - 
@article{
author = "Stanković, Dalibor M. and Milanović, Zorana and Švorc, Lubomir and Stanković, Vesna and Janković, Drina and Mirković, Marija D. and Vranješ-Đurić, Sanja",
year = "2021",
abstract = "This work presents advanced electrochemical platform based on screen printed diamond electrode (SPDE) system for the single drop “point-of-care” testing. Proposed approach was applied for the quantification of doxorubicin hydrochloride (DOX) in biological fluids and pharmaceutical product. Using a single drop (~30 μL) of the tested solution at the electrode surface, DOX showed high electroactivity over a wide range of pHs. In these conditions, single oval shaped, well-defined and pH dependent oxidation peak was observed in the potential range from 0.5 V to 1.3 V. In the reverse scan, two cathodic peaks, were noted – around 0.3 V and – 0.5 V. Similarly, first reduction peak was pH dependent, while second one was independent in the studied range. Experimental conditions for DOX quantification were optimized and natures of the electrode reactions were investigated. Working linear range obtained for DOX detection was from 0.1 to 2.5 μM. Diffusion controlled electrode reaction reveal long life time of the proposed electrode as well high potential for practical application. Developed procedure was successfully applied for the DOX analysis in biological fluids – urine and pharmaceutical formulation. Obtained results clearly indicated that given procedure can be easily implemented for pharmaceutical control and medical analysis, in both, laboratory and field conditions. © 2021 Elsevier B.V.",
journal = "Diamond and Related Materials",
title = "Screen printed diamond electrode as efficient “point-of-care” platform for submicromolar determination of cytostatic drug in biological fluids and pharmaceutical product",
volume = "113",
pages = "108277",
doi = "10.1016/j.diamond.2021.108277"
}
Stanković, D. M., Milanović, Z., Švorc, L., Stanković, V., Janković, D., Mirković, M. D.,& Vranješ-Đurić, S.. (2021). Screen printed diamond electrode as efficient “point-of-care” platform for submicromolar determination of cytostatic drug in biological fluids and pharmaceutical product. in Diamond and Related Materials, 113, 108277.
https://doi.org/10.1016/j.diamond.2021.108277
Stanković DM, Milanović Z, Švorc L, Stanković V, Janković D, Mirković MD, Vranješ-Đurić S. Screen printed diamond electrode as efficient “point-of-care” platform for submicromolar determination of cytostatic drug in biological fluids and pharmaceutical product. in Diamond and Related Materials. 2021;113:108277.
doi:10.1016/j.diamond.2021.108277 .
Stanković, Dalibor M., Milanović, Zorana, Švorc, Lubomir, Stanković, Vesna, Janković, Drina, Mirković, Marija D., Vranješ-Đurić, Sanja, "Screen printed diamond electrode as efficient “point-of-care” platform for submicromolar determination of cytostatic drug in biological fluids and pharmaceutical product" in Diamond and Related Materials, 113 (2021):108277,
https://doi.org/10.1016/j.diamond.2021.108277 . .
6
2
4

Bioevaluation of glucose-modified liposomes as a potential drug delivery system for cancer treatment using 177-Lu radiotracking

Cvjetinović, Đorđe; Prijović, Željko; Janković, Drina; Radović, Magdalena; Mirković, Marija D.; Milanović, Zorana; Mojović, Miloš; Škalamera, Đani; Vranješ-Đurić, Sanja

(2021)

TY  - JOUR
AU  - Cvjetinović, Đorđe
AU  - Prijović, Željko
AU  - Janković, Drina
AU  - Radović, Magdalena
AU  - Mirković, Marija D.
AU  - Milanović, Zorana
AU  - Mojović, Miloš
AU  - Škalamera, Đani
AU  - Vranješ-Đurić, Sanja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9151
AB  - Liposomes are promising drug's delivery systems due to decreased toxicity of the liposome-encapsulated drug, but wider clinical application requires their more efficient tumor targeting with uptake, controlled drug release and higher shelf life. The unique metabolic characteristics of cancer cells based on higher demand for energy and therefore increased glucose utilization were exploited in the design of glucose modified liposomes (GML) with the aim to provide increased tumor targeting via glucose transporters and increased ability of drug delivery into tumor cells. Tumor accumulating potential of GML and non-glucose liposomes (NGL) were investigated on CT26 and LS174T tumor-bearing mice by simple and reliable radiotracer method using 177Lu as radioactive marker. Both liposomes, GML and NGL were radiolabeled in high radiolabeling yield, showing high in vitro stability in biological media, as the main prerequisite for the biodistribution studies. Tumors displayed significantly better accumulation of 177Lu-GML with the maximum uptake 6 h post-injection (5.8 ± 0.2%/g in LS174T tumor and 5.1 ± 0.5%/g in CT26 tumor), compared to negligible uptake of 177Lu-NGL (0.6 ± 0.1%/g in LS174T tumor and 0.9 ± 0.2%/g in CT26 tumor). Results of comparative biodistribution studies of 177Lu-NGL and 177Lu-GML indicate that increased accumulation of GML is enabled by glucose transporters and subsequent endocytosis, resulting in their prolonged retention in tumor tissues (up to 72 h). Direct radiolabeling of liposomes with 177Lu may be used not only for biodistribution studies using radiotracking, but also for cancer treatment. © 2021 Elsevier B.V.
T2  - Journal of Controlled Release
T1  - Bioevaluation of glucose-modified liposomes as a potential drug delivery system for cancer treatment using 177-Lu radiotracking
VL  - 332
SP  - 301
EP  - 311
DO  - 10.1016/j.jconrel.2021.03.006
ER  - 
@article{
author = "Cvjetinović, Đorđe and Prijović, Željko and Janković, Drina and Radović, Magdalena and Mirković, Marija D. and Milanović, Zorana and Mojović, Miloš and Škalamera, Đani and Vranješ-Đurić, Sanja",
year = "2021",
abstract = "Liposomes are promising drug's delivery systems due to decreased toxicity of the liposome-encapsulated drug, but wider clinical application requires their more efficient tumor targeting with uptake, controlled drug release and higher shelf life. The unique metabolic characteristics of cancer cells based on higher demand for energy and therefore increased glucose utilization were exploited in the design of glucose modified liposomes (GML) with the aim to provide increased tumor targeting via glucose transporters and increased ability of drug delivery into tumor cells. Tumor accumulating potential of GML and non-glucose liposomes (NGL) were investigated on CT26 and LS174T tumor-bearing mice by simple and reliable radiotracer method using 177Lu as radioactive marker. Both liposomes, GML and NGL were radiolabeled in high radiolabeling yield, showing high in vitro stability in biological media, as the main prerequisite for the biodistribution studies. Tumors displayed significantly better accumulation of 177Lu-GML with the maximum uptake 6 h post-injection (5.8 ± 0.2%/g in LS174T tumor and 5.1 ± 0.5%/g in CT26 tumor), compared to negligible uptake of 177Lu-NGL (0.6 ± 0.1%/g in LS174T tumor and 0.9 ± 0.2%/g in CT26 tumor). Results of comparative biodistribution studies of 177Lu-NGL and 177Lu-GML indicate that increased accumulation of GML is enabled by glucose transporters and subsequent endocytosis, resulting in their prolonged retention in tumor tissues (up to 72 h). Direct radiolabeling of liposomes with 177Lu may be used not only for biodistribution studies using radiotracking, but also for cancer treatment. © 2021 Elsevier B.V.",
journal = "Journal of Controlled Release",
title = "Bioevaluation of glucose-modified liposomes as a potential drug delivery system for cancer treatment using 177-Lu radiotracking",
volume = "332",
pages = "301-311",
doi = "10.1016/j.jconrel.2021.03.006"
}
Cvjetinović, Đ., Prijović, Ž., Janković, D., Radović, M., Mirković, M. D., Milanović, Z., Mojović, M., Škalamera, Đ.,& Vranješ-Đurić, S.. (2021). Bioevaluation of glucose-modified liposomes as a potential drug delivery system for cancer treatment using 177-Lu radiotracking. in Journal of Controlled Release, 332, 301-311.
https://doi.org/10.1016/j.jconrel.2021.03.006
Cvjetinović Đ, Prijović Ž, Janković D, Radović M, Mirković MD, Milanović Z, Mojović M, Škalamera Đ, Vranješ-Đurić S. Bioevaluation of glucose-modified liposomes as a potential drug delivery system for cancer treatment using 177-Lu radiotracking. in Journal of Controlled Release. 2021;332:301-311.
doi:10.1016/j.jconrel.2021.03.006 .
Cvjetinović, Đorđe, Prijović, Željko, Janković, Drina, Radović, Magdalena, Mirković, Marija D., Milanović, Zorana, Mojović, Miloš, Škalamera, Đani, Vranješ-Đurić, Sanja, "Bioevaluation of glucose-modified liposomes as a potential drug delivery system for cancer treatment using 177-Lu radiotracking" in Journal of Controlled Release, 332 (2021):301-311,
https://doi.org/10.1016/j.jconrel.2021.03.006 . .
1
8
4
5

Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis

Milanović, Zorana; Beletić, Anđelo; Vekić, Jelena; Zeljković, Aleksandra; Andrić, Nenad; Ilić Božović, Anja; Spariosu, Kristina; Radaković, Milena; Ajtić, Jelena; Kovačević-Filipović, Milica M.

(2020)

TY  - JOUR
AU  - Milanović, Zorana
AU  - Beletić, Anđelo
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
AU  - Andrić, Nenad
AU  - Ilić Božović, Anja
AU  - Spariosu, Kristina
AU  - Radaković, Milena
AU  - Ajtić, Jelena
AU  - Kovačević-Filipović, Milica M.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9011
AB  - Asymptomatic outdoor dogs can be carriers of Babesia canis, but data describing the development of an acute phase response (APR) are not available. We hypothesised that these dogs have a moderate APR that could be detected by hematological and biochemical changes. Two groups of Babesia-exposed dogs were represented by nine B. canis PCR-positive and twenty B. canis PCR-negative, seroreactive dogs. The control group consisted of ten Babesia-naïve dogs. Serum amyloid A (SAA), paraoxonase-1 (PON-1), complete blood count, and biochemistry parameters were analysed by standard methodologies. Protein and lipoprotein fractions were separated using agarose gel electrophoresis (GE), and the dominant diameters of lipoproteins were assessed on gradient GE. Results were evaluated using non-parametric tests and the Receiver Operating Characteristic curve. SAA (median 39.0 μg/mL, range 2.2–48.8 μg/mL), total protein (median 74.7 g/L, range 57.1–98.3 g/L) and the dominant diameter of α-lipoproteins (median 13.31 nm, range 12.09–14.17 nm) in B. canis PCR-positive dogs were higher relative to dogs in the control group or dogs that were PCR-negative but seroreactive (p < 0.001 for both groups). Mild to moderate anemia (4/29), thrombocytopenia (7/29), and leukocyte counts that were close to the upper limit of the reference range were encountered in both Babesia-exposed groups. When compared to controls, Babesia-exposed dogs displayed decreased a PON-1 activity and protein GE pattern consistent with low-grade chronic inflammation (p < 0.001 for both groups). Dogs with detectable amounts of B. canis DNA in blood contain increased levels of SAA and total protein along with α-lipoproteins that display an increased diameter relative to those dogs with positive Babesia serology but undetectable levels of B. canis DNA in blood.
T2  - Veterinary Parasitology
T1  - Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis
VL  - 282
SP  - 109140
DO  - 10.1016/j.vetpar.2020.109140
ER  - 
@article{
author = "Milanović, Zorana and Beletić, Anđelo and Vekić, Jelena and Zeljković, Aleksandra and Andrić, Nenad and Ilić Božović, Anja and Spariosu, Kristina and Radaković, Milena and Ajtić, Jelena and Kovačević-Filipović, Milica M.",
year = "2020",
abstract = "Asymptomatic outdoor dogs can be carriers of Babesia canis, but data describing the development of an acute phase response (APR) are not available. We hypothesised that these dogs have a moderate APR that could be detected by hematological and biochemical changes. Two groups of Babesia-exposed dogs were represented by nine B. canis PCR-positive and twenty B. canis PCR-negative, seroreactive dogs. The control group consisted of ten Babesia-naïve dogs. Serum amyloid A (SAA), paraoxonase-1 (PON-1), complete blood count, and biochemistry parameters were analysed by standard methodologies. Protein and lipoprotein fractions were separated using agarose gel electrophoresis (GE), and the dominant diameters of lipoproteins were assessed on gradient GE. Results were evaluated using non-parametric tests and the Receiver Operating Characteristic curve. SAA (median 39.0 μg/mL, range 2.2–48.8 μg/mL), total protein (median 74.7 g/L, range 57.1–98.3 g/L) and the dominant diameter of α-lipoproteins (median 13.31 nm, range 12.09–14.17 nm) in B. canis PCR-positive dogs were higher relative to dogs in the control group or dogs that were PCR-negative but seroreactive (p < 0.001 for both groups). Mild to moderate anemia (4/29), thrombocytopenia (7/29), and leukocyte counts that were close to the upper limit of the reference range were encountered in both Babesia-exposed groups. When compared to controls, Babesia-exposed dogs displayed decreased a PON-1 activity and protein GE pattern consistent with low-grade chronic inflammation (p < 0.001 for both groups). Dogs with detectable amounts of B. canis DNA in blood contain increased levels of SAA and total protein along with α-lipoproteins that display an increased diameter relative to those dogs with positive Babesia serology but undetectable levels of B. canis DNA in blood.",
journal = "Veterinary Parasitology",
title = "Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis",
volume = "282",
pages = "109140",
doi = "10.1016/j.vetpar.2020.109140"
}
Milanović, Z., Beletić, A., Vekić, J., Zeljković, A., Andrić, N., Ilić Božović, A., Spariosu, K., Radaković, M., Ajtić, J.,& Kovačević-Filipović, M. M.. (2020). Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis. in Veterinary Parasitology, 282, 109140.
https://doi.org/10.1016/j.vetpar.2020.109140
Milanović Z, Beletić A, Vekić J, Zeljković A, Andrić N, Ilić Božović A, Spariosu K, Radaković M, Ajtić J, Kovačević-Filipović MM. Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis. in Veterinary Parasitology. 2020;282:109140.
doi:10.1016/j.vetpar.2020.109140 .
Milanović, Zorana, Beletić, Anđelo, Vekić, Jelena, Zeljković, Aleksandra, Andrić, Nenad, Ilić Božović, Anja, Spariosu, Kristina, Radaković, Milena, Ajtić, Jelena, Kovačević-Filipović, Milica M., "Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis" in Veterinary Parasitology, 282 (2020):109140,
https://doi.org/10.1016/j.vetpar.2020.109140 . .
1
3
1
2

A single drop histamine sensor based on AuNPs/MnO2 modified screen-printed electrode

Knežević, Sara; Ognjanović, Miloš; Nedić, Nemanja; Mariano, Jose F. M. L.; Milanović, Zorana; Petković, Branka B.; Antić, Bratislav; Vranješ-Đurić, Sanja; Stanković, Dalibor M.

(2020)

TY  - JOUR
AU  - Knežević, Sara
AU  - Ognjanović, Miloš
AU  - Nedić, Nemanja
AU  - Mariano, Jose F. M. L.
AU  - Milanović, Zorana
AU  - Petković, Branka B.
AU  - Antić, Bratislav
AU  - Vranješ-Đurić, Sanja
AU  - Stanković, Dalibor M.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8834
AB  - In order to ensure high food quality, one of the prime importance is the detection and quantification of histamine, well known marine food poison. In this work, we constructed novel electrochemical biosensor for the detection of histamine based on gold nanoparticles decorated on manganese dioxide (Au/MnO2) and used for modification of screen-printed carbon electrode (Au/MnO2@SPCE). The constructed sensor was then used for the estimation of histamine content in a single drop. Materials used in this study were synthesized and characterized using HR-TEM, XRPD and electrochemical methods. The amperometric detection method was optimized and, under selected operating parameters (supporting electrolyte pH 6, working potential of 1 V), the proposed sensor possesses linear working range from 0.3 µM to 5.1 µM, with a detection limit of 0.08 µM. The effect of selected interferences was investigated and it was found that the developed approach offers accurate, precise, selective, fast and reproducible quantification of histamine using only one drop of the sample. In the end, this work stands as a proof-of-concept of the modified electrodes and electrochemical detection as a promising and prospective approach for the applications in real-time monitoring of the food quality.
T2  - Microchemical Journal
T1  - A single drop histamine sensor based on AuNPs/MnO2 modified screen-printed electrode
VL  - 155
SP  - 104778
DO  - 10.1016/j.microc.2020.104778
ER  - 
@article{
author = "Knežević, Sara and Ognjanović, Miloš and Nedić, Nemanja and Mariano, Jose F. M. L. and Milanović, Zorana and Petković, Branka B. and Antić, Bratislav and Vranješ-Đurić, Sanja and Stanković, Dalibor M.",
year = "2020",
abstract = "In order to ensure high food quality, one of the prime importance is the detection and quantification of histamine, well known marine food poison. In this work, we constructed novel electrochemical biosensor for the detection of histamine based on gold nanoparticles decorated on manganese dioxide (Au/MnO2) and used for modification of screen-printed carbon electrode (Au/MnO2@SPCE). The constructed sensor was then used for the estimation of histamine content in a single drop. Materials used in this study were synthesized and characterized using HR-TEM, XRPD and electrochemical methods. The amperometric detection method was optimized and, under selected operating parameters (supporting electrolyte pH 6, working potential of 1 V), the proposed sensor possesses linear working range from 0.3 µM to 5.1 µM, with a detection limit of 0.08 µM. The effect of selected interferences was investigated and it was found that the developed approach offers accurate, precise, selective, fast and reproducible quantification of histamine using only one drop of the sample. In the end, this work stands as a proof-of-concept of the modified electrodes and electrochemical detection as a promising and prospective approach for the applications in real-time monitoring of the food quality.",
journal = "Microchemical Journal",
title = "A single drop histamine sensor based on AuNPs/MnO2 modified screen-printed electrode",
volume = "155",
pages = "104778",
doi = "10.1016/j.microc.2020.104778"
}
Knežević, S., Ognjanović, M., Nedić, N., Mariano, J. F. M. L., Milanović, Z., Petković, B. B., Antić, B., Vranješ-Đurić, S.,& Stanković, D. M.. (2020). A single drop histamine sensor based on AuNPs/MnO2 modified screen-printed electrode. in Microchemical Journal, 155, 104778.
https://doi.org/10.1016/j.microc.2020.104778
Knežević S, Ognjanović M, Nedić N, Mariano JFML, Milanović Z, Petković BB, Antić B, Vranješ-Đurić S, Stanković DM. A single drop histamine sensor based on AuNPs/MnO2 modified screen-printed electrode. in Microchemical Journal. 2020;155:104778.
doi:10.1016/j.microc.2020.104778 .
Knežević, Sara, Ognjanović, Miloš, Nedić, Nemanja, Mariano, Jose F. M. L., Milanović, Zorana, Petković, Branka B., Antić, Bratislav, Vranješ-Đurić, Sanja, Stanković, Dalibor M., "A single drop histamine sensor based on AuNPs/MnO2 modified screen-printed electrode" in Microchemical Journal, 155 (2020):104778,
https://doi.org/10.1016/j.microc.2020.104778 . .
1
13
9
9

Aminosilanized flower-structured superparamagnetic iron oxide nanoparticles coupled to 131I-labeled CC49 antibody for combined radionuclide and hyperthermia therapy of cancer

Stanković, Aljoša; Mihailović, Jasna; Mirković, Marija D.; Radović, Magdalena; Milanović, Zorana; Ognjanović, Miloš; Janković, Drina; Antić, Bratislav; Mijović, Milica; Vranješ-Đurić, Sanja; Prijović, Željko

(2020)

TY  - JOUR
AU  - Stanković, Aljoša
AU  - Mihailović, Jasna
AU  - Mirković, Marija D.
AU  - Radović, Magdalena
AU  - Milanović, Zorana
AU  - Ognjanović, Miloš
AU  - Janković, Drina
AU  - Antić, Bratislav
AU  - Mijović, Milica
AU  - Vranješ-Đurić, Sanja
AU  - Prijović, Željko
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9117
AB  - Combined radionuclide therapy with magnetic nanoparticles-mediated hyperthermia has been under research focus as a promising tumor therapy approach. The objective of this study was to investigate the potential of 131I-radiolabeled superparamagnetic iron oxide nanoparticles (SPIONs) prepared as the ~40 nm flower-shaped structures with excellent heating efficiency (specific absorption rate at H0 = 15.9 kA∙m−1 and resonant frequency of 252 kHz was 123.1 W∙g−1) for nano-brachytherapy of tumors. 131I-radiolabeled CC49 antibody attached to SPIONs via reactive groups of 3-aminopropyltriethoxysilane (APTES) provided specificity and long-lasting localized retention after their intratumoral application into LS174T human colon adenocarcinoma xenografts in NOD-SCID mice. The results demonstrate feasibility and effectiveness of magnetic hyperthermia (HT), radionuclide therapy (RT) and their combination (HT + RT) in treating cancer in xenograft models. Combined therapy approach induced a significant (p < 0.01) tumor growth suppression in comparison to untreated groups presented by the tumor volume inhibitory rate (TVIR): 54.38%, 68.77%, 73.00% for HT, RT and HT + RT, respectively in comparison to untreated group and 48.31%, 64,62% and 69,41%, respectively, for the SPIONs-only injected group. Histopathology analysis proved the necrosis and apoptosis in treated tumors without general toxicity. Obtained data support the idea that nano-brachytherapy combined with hyperthermia is a promising approach for effective cancer treatment.
T2  - International Journal of Pharmaceutics
T1  - Aminosilanized flower-structured superparamagnetic iron oxide nanoparticles coupled to 131I-labeled CC49 antibody for combined radionuclide and hyperthermia therapy of cancer
VL  - 587
SP  - 119628
DO  - 10.1016/j.ijpharm.2020.119628
ER  - 
@article{
author = "Stanković, Aljoša and Mihailović, Jasna and Mirković, Marija D. and Radović, Magdalena and Milanović, Zorana and Ognjanović, Miloš and Janković, Drina and Antić, Bratislav and Mijović, Milica and Vranješ-Đurić, Sanja and Prijović, Željko",
year = "2020",
abstract = "Combined radionuclide therapy with magnetic nanoparticles-mediated hyperthermia has been under research focus as a promising tumor therapy approach. The objective of this study was to investigate the potential of 131I-radiolabeled superparamagnetic iron oxide nanoparticles (SPIONs) prepared as the ~40 nm flower-shaped structures with excellent heating efficiency (specific absorption rate at H0 = 15.9 kA∙m−1 and resonant frequency of 252 kHz was 123.1 W∙g−1) for nano-brachytherapy of tumors. 131I-radiolabeled CC49 antibody attached to SPIONs via reactive groups of 3-aminopropyltriethoxysilane (APTES) provided specificity and long-lasting localized retention after their intratumoral application into LS174T human colon adenocarcinoma xenografts in NOD-SCID mice. The results demonstrate feasibility and effectiveness of magnetic hyperthermia (HT), radionuclide therapy (RT) and their combination (HT + RT) in treating cancer in xenograft models. Combined therapy approach induced a significant (p < 0.01) tumor growth suppression in comparison to untreated groups presented by the tumor volume inhibitory rate (TVIR): 54.38%, 68.77%, 73.00% for HT, RT and HT + RT, respectively in comparison to untreated group and 48.31%, 64,62% and 69,41%, respectively, for the SPIONs-only injected group. Histopathology analysis proved the necrosis and apoptosis in treated tumors without general toxicity. Obtained data support the idea that nano-brachytherapy combined with hyperthermia is a promising approach for effective cancer treatment.",
journal = "International Journal of Pharmaceutics",
title = "Aminosilanized flower-structured superparamagnetic iron oxide nanoparticles coupled to 131I-labeled CC49 antibody for combined radionuclide and hyperthermia therapy of cancer",
volume = "587",
pages = "119628",
doi = "10.1016/j.ijpharm.2020.119628"
}
Stanković, A., Mihailović, J., Mirković, M. D., Radović, M., Milanović, Z., Ognjanović, M., Janković, D., Antić, B., Mijović, M., Vranješ-Đurić, S.,& Prijović, Ž.. (2020). Aminosilanized flower-structured superparamagnetic iron oxide nanoparticles coupled to 131I-labeled CC49 antibody for combined radionuclide and hyperthermia therapy of cancer. in International Journal of Pharmaceutics, 587, 119628.
https://doi.org/10.1016/j.ijpharm.2020.119628
Stanković A, Mihailović J, Mirković MD, Radović M, Milanović Z, Ognjanović M, Janković D, Antić B, Mijović M, Vranješ-Đurić S, Prijović Ž. Aminosilanized flower-structured superparamagnetic iron oxide nanoparticles coupled to 131I-labeled CC49 antibody for combined radionuclide and hyperthermia therapy of cancer. in International Journal of Pharmaceutics. 2020;587:119628.
doi:10.1016/j.ijpharm.2020.119628 .
Stanković, Aljoša, Mihailović, Jasna, Mirković, Marija D., Radović, Magdalena, Milanović, Zorana, Ognjanović, Miloš, Janković, Drina, Antić, Bratislav, Mijović, Milica, Vranješ-Đurić, Sanja, Prijović, Željko, "Aminosilanized flower-structured superparamagnetic iron oxide nanoparticles coupled to 131I-labeled CC49 antibody for combined radionuclide and hyperthermia therapy of cancer" in International Journal of Pharmaceutics, 587 (2020):119628,
https://doi.org/10.1016/j.ijpharm.2020.119628 . .
8
6
9

Optimization of the radiolabelling method for improved in vitro and in vivo stability of 90Y-albumin microspheres

Vukadinović, Aleksandar; Janković, Drina; Radović, Magdalena; Milanović, Zorana; Mirković, Marija D.; Stanković, Dragana; Vranješ-Đurić, Sanja

(2020)

TY  - JOUR
AU  - Vukadinović, Aleksandar
AU  - Janković, Drina
AU  - Radović, Magdalena
AU  - Milanović, Zorana
AU  - Mirković, Marija D.
AU  - Stanković, Dragana
AU  - Vranješ-Đurić, Sanja
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8652
AB  - Biologically stable 90Y-labelled albumin microspheres (AMS) were developed by optimizing the process of their preparation. Three formulations of 90Y-AMS were initially prepared with high radiolabelling yield but depending on the step when the radionuclide 90Y and DTPA chelator were added, radiolabelled microspheres with different in vitro and in vivo stability were obtained. DTPA was proved as a useful chelating agent that tightly links radionuclide 90Y to albumin. Also, AMS radiolabelled via DTPA during preparation and before microspheres stabilization, showed significant in vitro and in vivo stability ready for the potential use in selective internal radiation therapy. © 2019 Elsevier Ltd
T2  - Applied Radiation and Isotopes
T1  - Optimization of the radiolabelling method for improved in vitro and in vivo stability of 90Y-albumin microspheres
VL  - 156
SP  - 108984
DO  - 10.1016/j.apradiso.2019.108984
ER  - 
@article{
author = "Vukadinović, Aleksandar and Janković, Drina and Radović, Magdalena and Milanović, Zorana and Mirković, Marija D. and Stanković, Dragana and Vranješ-Đurić, Sanja",
year = "2020",
abstract = "Biologically stable 90Y-labelled albumin microspheres (AMS) were developed by optimizing the process of their preparation. Three formulations of 90Y-AMS were initially prepared with high radiolabelling yield but depending on the step when the radionuclide 90Y and DTPA chelator were added, radiolabelled microspheres with different in vitro and in vivo stability were obtained. DTPA was proved as a useful chelating agent that tightly links radionuclide 90Y to albumin. Also, AMS radiolabelled via DTPA during preparation and before microspheres stabilization, showed significant in vitro and in vivo stability ready for the potential use in selective internal radiation therapy. © 2019 Elsevier Ltd",
journal = "Applied Radiation and Isotopes",
title = "Optimization of the radiolabelling method for improved in vitro and in vivo stability of 90Y-albumin microspheres",
volume = "156",
pages = "108984",
doi = "10.1016/j.apradiso.2019.108984"
}
Vukadinović, A., Janković, D., Radović, M., Milanović, Z., Mirković, M. D., Stanković, D.,& Vranješ-Đurić, S.. (2020). Optimization of the radiolabelling method for improved in vitro and in vivo stability of 90Y-albumin microspheres. in Applied Radiation and Isotopes, 156, 108984.
https://doi.org/10.1016/j.apradiso.2019.108984
Vukadinović A, Janković D, Radović M, Milanović Z, Mirković MD, Stanković D, Vranješ-Đurić S. Optimization of the radiolabelling method for improved in vitro and in vivo stability of 90Y-albumin microspheres. in Applied Radiation and Isotopes. 2020;156:108984.
doi:10.1016/j.apradiso.2019.108984 .
Vukadinović, Aleksandar, Janković, Drina, Radović, Magdalena, Milanović, Zorana, Mirković, Marija D., Stanković, Dragana, Vranješ-Đurić, Sanja, "Optimization of the radiolabelling method for improved in vitro and in vivo stability of 90Y-albumin microspheres" in Applied Radiation and Isotopes, 156 (2020):108984,
https://doi.org/10.1016/j.apradiso.2019.108984 . .
1
1
2

Procena merne nesigurnosti pri merenju aktivnosti radiofarmaceutika u doze kalibratoru

Janković, Drina; Mirković, Marija D.; Radović, Magdalena; Milanović, Zorana; Perić, Marko R.; Vukadinović, Aleksandar; Vranješ-Đurić, Sanja

(Београд : Институт за нуклеарне науке "Винча" : Друштво за заштиту од зрачења Србије и Црне Горе, 2019)

TY  - CONF
AU  - Janković, Drina
AU  - Mirković, Marija D.
AU  - Radović, Magdalena
AU  - Milanović, Zorana
AU  - Perić, Marko R.
AU  - Vukadinović, Aleksandar
AU  - Vranješ-Đurić, Sanja
PY  - 2019
UR  - https://plus.sr.cobiss.net/opac7/bib/279687436
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8710
AB  - Doze kalibrator je jedan od osnovnih uređaja u nuklearnoj medicini, koji se koristi za merenje aktivnosti (doza) radiofarmaceutika koji se daju pacijentima bilo u dijagnostičke ili u terapijske svrhe. Aktivnosti gama i beta emitera različitih energija moraju da budu izmerene što tačnije da bi izlaganja ljudi (pacijenata) jonizujućem zračenju bila svedena na najmanji mogući nivo, a da se dobiju klinički značajni rezultati. Početna tačnost kalibratora (nesigurnost 5% ili manje) može se vremenom menjati kao rezultat promene pritiska u jonizacionoj komori ili električnog drifta. Zbog toga kontrola kvaliteta doze kalibratora treba da se sprovodi rutinski kako bi se osigurala tačnost i sledljivost merenja.U radu su prikazani rezultati procene nesigurnostipri merenjima aktivnosti radiofarmaceutika u doze kalibratoru u Laboratoriji za radioizotope. Komponente nesigurnosti, koje su važne za ova merenja, identifikuju se i uzimaju u obzir prilikom procene merne nesigurnosti. Razumevanje izvora nesigurnosti i korišćenje odgovarajućih korekcionih faktora mogu minimizirati netačna merenja.
AB  - A dose calibrator is an essential device in a nuclear medicine, utilized for measurement the activity of radiopharmaceuticals administered to patients both for diagnostic and therapeutic purposes. It has to measurethe radioactivity of gamma and beta with different energies precisely for high quality imaging and for applying the right amount of radiation to treat disease. Initial accuracy (uncertainty 5% or less) may change with time as a result of changing pressureof the chamber gas and slow electrical drift. The quality controls should be undertaken on a routine basis to ensure the accuracy and traceability of measurements of the activities of radiopharmaceuticals.The paper presents the results of estimation of uncertainty in the measurement of the activity in the dose calibrator in the Laboratory for radioisotopes. The uncertainty components, that are important for these measurements, are identified and taken into account while estimating the uncertainty of measurement. Understanding the source of uncertainty and using appropriate techniques can minimize inaccurate measurements.
PB  - Београд : Институт за нуклеарне науке "Винча" : Друштво за заштиту од зрачења Србије и Црне Горе
C3  - 30. симпозијум ДЗЗСЦГ : зборник радова
T1  - Procena merne nesigurnosti pri merenju aktivnosti radiofarmaceutika u doze kalibratoru
T1  - Estimation of measurement uncertainty in measuring radiopharmaceutical activity in dose calibrator
SP  - 366
EP  - 372
ER  - 
@conference{
author = "Janković, Drina and Mirković, Marija D. and Radović, Magdalena and Milanović, Zorana and Perić, Marko R. and Vukadinović, Aleksandar and Vranješ-Đurić, Sanja",
year = "2019",
abstract = "Doze kalibrator je jedan od osnovnih uređaja u nuklearnoj medicini, koji se koristi za merenje aktivnosti (doza) radiofarmaceutika koji se daju pacijentima bilo u dijagnostičke ili u terapijske svrhe. Aktivnosti gama i beta emitera različitih energija moraju da budu izmerene što tačnije da bi izlaganja ljudi (pacijenata) jonizujućem zračenju bila svedena na najmanji mogući nivo, a da se dobiju klinički značajni rezultati. Početna tačnost kalibratora (nesigurnost 5% ili manje) može se vremenom menjati kao rezultat promene pritiska u jonizacionoj komori ili električnog drifta. Zbog toga kontrola kvaliteta doze kalibratora treba da se sprovodi rutinski kako bi se osigurala tačnost i sledljivost merenja.U radu su prikazani rezultati procene nesigurnostipri merenjima aktivnosti radiofarmaceutika u doze kalibratoru u Laboratoriji za radioizotope. Komponente nesigurnosti, koje su važne za ova merenja, identifikuju se i uzimaju u obzir prilikom procene merne nesigurnosti. Razumevanje izvora nesigurnosti i korišćenje odgovarajućih korekcionih faktora mogu minimizirati netačna merenja., A dose calibrator is an essential device in a nuclear medicine, utilized for measurement the activity of radiopharmaceuticals administered to patients both for diagnostic and therapeutic purposes. It has to measurethe radioactivity of gamma and beta with different energies precisely for high quality imaging and for applying the right amount of radiation to treat disease. Initial accuracy (uncertainty 5% or less) may change with time as a result of changing pressureof the chamber gas and slow electrical drift. The quality controls should be undertaken on a routine basis to ensure the accuracy and traceability of measurements of the activities of radiopharmaceuticals.The paper presents the results of estimation of uncertainty in the measurement of the activity in the dose calibrator in the Laboratory for radioisotopes. The uncertainty components, that are important for these measurements, are identified and taken into account while estimating the uncertainty of measurement. Understanding the source of uncertainty and using appropriate techniques can minimize inaccurate measurements.",
publisher = "Београд : Институт за нуклеарне науке "Винча" : Друштво за заштиту од зрачења Србије и Црне Горе",
journal = "30. симпозијум ДЗЗСЦГ : зборник радова",
title = "Procena merne nesigurnosti pri merenju aktivnosti radiofarmaceutika u doze kalibratoru, Estimation of measurement uncertainty in measuring radiopharmaceutical activity in dose calibrator",
pages = "366-372"
}
Janković, D., Mirković, M. D., Radović, M., Milanović, Z., Perić, M. R., Vukadinović, A.,& Vranješ-Đurić, S.. (2019). Procena merne nesigurnosti pri merenju aktivnosti radiofarmaceutika u doze kalibratoru. in 30. симпозијум ДЗЗСЦГ : зборник радова
Београд : Институт за нуклеарне науке "Винча" : Друштво за заштиту од зрачења Србије и Црне Горе., 366-372.
Janković D, Mirković MD, Radović M, Milanović Z, Perić MR, Vukadinović A, Vranješ-Đurić S. Procena merne nesigurnosti pri merenju aktivnosti radiofarmaceutika u doze kalibratoru. in 30. симпозијум ДЗЗСЦГ : зборник радова. 2019;:366-372..
Janković, Drina, Mirković, Marija D., Radović, Magdalena, Milanović, Zorana, Perić, Marko R., Vukadinović, Aleksandar, Vranješ-Đurić, Sanja, "Procena merne nesigurnosti pri merenju aktivnosti radiofarmaceutika u doze kalibratoru" in 30. симпозијум ДЗЗСЦГ : зборник радова (2019):366-372.

99mTc–bisphosphonate–coated magnetic nanoparticles as potential theranostic nanoagent

Mirković, Marija D.; Radović, Magdalena; Stanković, Dragana; Milanović, Zorana; Janković, Drina; Matović, Milovan D.; Jeremić, Marija; Antić, Bratislav; Vranješ-Đurić, Sanja

(2019)

TY  - JOUR
AU  - Mirković, Marija D.
AU  - Radović, Magdalena
AU  - Stanković, Dragana
AU  - Milanović, Zorana
AU  - Janković, Drina
AU  - Matović, Milovan D.
AU  - Jeremić, Marija
AU  - Antić, Bratislav
AU  - Vranješ-Đurić, Sanja
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8156
AB  - Novel theranostic nanoplatform is expected to integrate imaging for guiding and monitoring of the tumor therapy with great therapeutic efficacy and fewer side effects. Here we describe the preparation of a multifunctional 99mTc–bisphosphonate–coated magnetic nanoparticles (MNPs) based on Fe3O4 and coated with two hydrophilic bisphosphonate ligands, i.e., methylene diphosphonate (MDP) and 1–hydroxyethane-1,1- diphosphonate (HEDP). The presence of the bisphosphonates on the MNPs surface, enabled their biocompatibility, colloidal stability and successful binding of the radionuclide. The morphology, size, structure, surface charge and magnetic properties of obtained bisphosphonate–coated Fe3O4 MNPs were characterized by transmission electron microscopy, X–ray powder diffraction, dynamic light scattering, laser Doppler electrophoresis, Fourier transform infrared spectroscopy and vibrating sample magnetometer. The specific power absorption values for Fe3O4–MDP and Fe3O4–HEDP were 113 W/g and 141 W/g, respectively, indicated their heating ability under applied magnetic field. Coated MNPs were radiolabeled with 99mTc using stannous chloride as the reducing agent in a reproducible high yield (95% for Fe3O4–MDP and 97% for Fe3O4–HEDP MNPs) and were remained stable in saline and human serum for 24 h. Ex vivo biodistribution studies presented significant liver and spleen uptake in healthy Wistar rats after intravenous administration at all examined time points due to the colloidal nature of both 99mTc–MNPs. Results of scintigraphy studies are in accordance with ex vivo biodistribution studies, demonstrating high in vivo stability of radiolabeled MNPs and therefore results of both methods were proved as accurate information on the biodistribution profile of investigated MNPs. Overall, in vitro and in vivo stability as well as heating ability, indicate that biocompatible radiolabeled bisphosphonate magnetic nanoparticles exhibit promising potential as a theranostic nanoagent. © 2019 Elsevier B.V.
T2  - Materials Science and Engineering: C
T1  - 99mTc–bisphosphonate–coated magnetic nanoparticles as potential theranostic nanoagent
VL  - 102
SP  - 124
EP  - 133
DO  - 10.1016/j.msec.2019.04.034
ER  - 
@article{
author = "Mirković, Marija D. and Radović, Magdalena and Stanković, Dragana and Milanović, Zorana and Janković, Drina and Matović, Milovan D. and Jeremić, Marija and Antić, Bratislav and Vranješ-Đurić, Sanja",
year = "2019",
abstract = "Novel theranostic nanoplatform is expected to integrate imaging for guiding and monitoring of the tumor therapy with great therapeutic efficacy and fewer side effects. Here we describe the preparation of a multifunctional 99mTc–bisphosphonate–coated magnetic nanoparticles (MNPs) based on Fe3O4 and coated with two hydrophilic bisphosphonate ligands, i.e., methylene diphosphonate (MDP) and 1–hydroxyethane-1,1- diphosphonate (HEDP). The presence of the bisphosphonates on the MNPs surface, enabled their biocompatibility, colloidal stability and successful binding of the radionuclide. The morphology, size, structure, surface charge and magnetic properties of obtained bisphosphonate–coated Fe3O4 MNPs were characterized by transmission electron microscopy, X–ray powder diffraction, dynamic light scattering, laser Doppler electrophoresis, Fourier transform infrared spectroscopy and vibrating sample magnetometer. The specific power absorption values for Fe3O4–MDP and Fe3O4–HEDP were 113 W/g and 141 W/g, respectively, indicated their heating ability under applied magnetic field. Coated MNPs were radiolabeled with 99mTc using stannous chloride as the reducing agent in a reproducible high yield (95% for Fe3O4–MDP and 97% for Fe3O4–HEDP MNPs) and were remained stable in saline and human serum for 24 h. Ex vivo biodistribution studies presented significant liver and spleen uptake in healthy Wistar rats after intravenous administration at all examined time points due to the colloidal nature of both 99mTc–MNPs. Results of scintigraphy studies are in accordance with ex vivo biodistribution studies, demonstrating high in vivo stability of radiolabeled MNPs and therefore results of both methods were proved as accurate information on the biodistribution profile of investigated MNPs. Overall, in vitro and in vivo stability as well as heating ability, indicate that biocompatible radiolabeled bisphosphonate magnetic nanoparticles exhibit promising potential as a theranostic nanoagent. © 2019 Elsevier B.V.",
journal = "Materials Science and Engineering: C",
title = "99mTc–bisphosphonate–coated magnetic nanoparticles as potential theranostic nanoagent",
volume = "102",
pages = "124-133",
doi = "10.1016/j.msec.2019.04.034"
}
Mirković, M. D., Radović, M., Stanković, D., Milanović, Z., Janković, D., Matović, M. D., Jeremić, M., Antić, B.,& Vranješ-Đurić, S.. (2019). 99mTc–bisphosphonate–coated magnetic nanoparticles as potential theranostic nanoagent. in Materials Science and Engineering: C, 102, 124-133.
https://doi.org/10.1016/j.msec.2019.04.034
Mirković MD, Radović M, Stanković D, Milanović Z, Janković D, Matović MD, Jeremić M, Antić B, Vranješ-Đurić S. 99mTc–bisphosphonate–coated magnetic nanoparticles as potential theranostic nanoagent. in Materials Science and Engineering: C. 2019;102:124-133.
doi:10.1016/j.msec.2019.04.034 .
Mirković, Marija D., Radović, Magdalena, Stanković, Dragana, Milanović, Zorana, Janković, Drina, Matović, Milovan D., Jeremić, Marija, Antić, Bratislav, Vranješ-Đurić, Sanja, "99mTc–bisphosphonate–coated magnetic nanoparticles as potential theranostic nanoagent" in Materials Science and Engineering: C, 102 (2019):124-133,
https://doi.org/10.1016/j.msec.2019.04.034 . .
16
15
15

Toxicity study of DE-EDCP as a potential drug for cancer therapy: Toxicity profile of DE-EDCP

Stanković, Dragana; Ristić, Slavica M.; Vukadinović, Aleksandar; Mirković, Marija D.; Vladimirov, Sandra S.; Milanović, Zorana; Radović, Magdalena; Mijović, Milica; Stanković, Dalibor M.; Sabo, Tibor J.; Vranješ-Đurić, Sanja; Janković, Drina

(2019)

TY  - JOUR
AU  - Stanković, Dragana
AU  - Ristić, Slavica M.
AU  - Vukadinović, Aleksandar
AU  - Mirković, Marija D.
AU  - Vladimirov, Sandra S.
AU  - Milanović, Zorana
AU  - Radović, Magdalena
AU  - Mijović, Milica
AU  - Stanković, Dalibor M.
AU  - Sabo, Tibor J.
AU  - Vranješ-Đurić, Sanja
AU  - Janković, Drina
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8492
AB  - It was reported that novel O,O′-diethyl-(S, S)-ethylenediamine-N,N′-di-2-(3-cyclohexyl) propanoate dihydrochloride (DE-EDCP) displayed in vitro antiproliferative activity on several human and mouse cancer cell lines, which was comparable to that of the prototypical anticancer drug cisplatin. In order to reveal its toxicity profile, acute and repeated-dose toxicity studies were performed in Naval Medical Research Institute (NMRI) Han mice. The intravenous LD50 values of DE-EDCP were found to be 95.3 and 101.3 mg/kg body weight in female and male mice, respectively. In the subacute toxicity study, DE-EDCP was administered intravenously at the doses of 15, 25, and 40 mg/kg/day for a period of 28 days. There were no adverse effects on general condition, growth, feed and water consumption, and hematological parameters. There was a significant increase in urea and alanine aminotransferase in female mice and aspartate aminotransferase and alkaline phosphatase in both genders in 40 mg/kg/day dose-treated group. The histopathological changes confined to the liver and kidney, but in other organs were not found. Satellite group revealed that changes in the kidney and liver were less pronounced, suggesting their reversibility. Interactions with DNA could also be of importance for understanding DE-EDCP toxic side effects. Hyperchromic effect obtained with ultraviolet–visible, suggested electrostatic interactions between DE-EDCP and calf thymus DNA. The toxicity testing of DE-EDCP was conducted to predict human outcomes. © The Author(s) 2018.
T2  - Human & Experimental Toxicology
T1  - Toxicity study of DE-EDCP as a potential drug for cancer therapy: Toxicity profile of DE-EDCP
VL  - 38
IS  - 4
SP  - 466
EP  - 481
DO  - 10.1177/0960327118819047
ER  - 
@article{
author = "Stanković, Dragana and Ristić, Slavica M. and Vukadinović, Aleksandar and Mirković, Marija D. and Vladimirov, Sandra S. and Milanović, Zorana and Radović, Magdalena and Mijović, Milica and Stanković, Dalibor M. and Sabo, Tibor J. and Vranješ-Đurić, Sanja and Janković, Drina",
year = "2019",
abstract = "It was reported that novel O,O′-diethyl-(S, S)-ethylenediamine-N,N′-di-2-(3-cyclohexyl) propanoate dihydrochloride (DE-EDCP) displayed in vitro antiproliferative activity on several human and mouse cancer cell lines, which was comparable to that of the prototypical anticancer drug cisplatin. In order to reveal its toxicity profile, acute and repeated-dose toxicity studies were performed in Naval Medical Research Institute (NMRI) Han mice. The intravenous LD50 values of DE-EDCP were found to be 95.3 and 101.3 mg/kg body weight in female and male mice, respectively. In the subacute toxicity study, DE-EDCP was administered intravenously at the doses of 15, 25, and 40 mg/kg/day for a period of 28 days. There were no adverse effects on general condition, growth, feed and water consumption, and hematological parameters. There was a significant increase in urea and alanine aminotransferase in female mice and aspartate aminotransferase and alkaline phosphatase in both genders in 40 mg/kg/day dose-treated group. The histopathological changes confined to the liver and kidney, but in other organs were not found. Satellite group revealed that changes in the kidney and liver were less pronounced, suggesting their reversibility. Interactions with DNA could also be of importance for understanding DE-EDCP toxic side effects. Hyperchromic effect obtained with ultraviolet–visible, suggested electrostatic interactions between DE-EDCP and calf thymus DNA. The toxicity testing of DE-EDCP was conducted to predict human outcomes. © The Author(s) 2018.",
journal = "Human & Experimental Toxicology",
title = "Toxicity study of DE-EDCP as a potential drug for cancer therapy: Toxicity profile of DE-EDCP",
volume = "38",
number = "4",
pages = "466-481",
doi = "10.1177/0960327118819047"
}
Stanković, D., Ristić, S. M., Vukadinović, A., Mirković, M. D., Vladimirov, S. S., Milanović, Z., Radović, M., Mijović, M., Stanković, D. M., Sabo, T. J., Vranješ-Đurić, S.,& Janković, D.. (2019). Toxicity study of DE-EDCP as a potential drug for cancer therapy: Toxicity profile of DE-EDCP. in Human & Experimental Toxicology, 38(4), 466-481.
https://doi.org/10.1177/0960327118819047
Stanković D, Ristić SM, Vukadinović A, Mirković MD, Vladimirov SS, Milanović Z, Radović M, Mijović M, Stanković DM, Sabo TJ, Vranješ-Đurić S, Janković D. Toxicity study of DE-EDCP as a potential drug for cancer therapy: Toxicity profile of DE-EDCP. in Human & Experimental Toxicology. 2019;38(4):466-481.
doi:10.1177/0960327118819047 .
Stanković, Dragana, Ristić, Slavica M., Vukadinović, Aleksandar, Mirković, Marija D., Vladimirov, Sandra S., Milanović, Zorana, Radović, Magdalena, Mijović, Milica, Stanković, Dalibor M., Sabo, Tibor J., Vranješ-Đurić, Sanja, Janković, Drina, "Toxicity study of DE-EDCP as a potential drug for cancer therapy: Toxicity profile of DE-EDCP" in Human & Experimental Toxicology, 38, no. 4 (2019):466-481,
https://doi.org/10.1177/0960327118819047 . .
1

Preparation and radiolabeling of surface-modified magnetic nanoparticles with technetium-99m as potential radiopharmaceuticals in nuclear medicine

Vukadinović, Aleksandar; Janković, Drina; Mirković, M; Radović, Magdalena; Perić, Marko R.; Milanović, Zorana; Stanković, D; Vranješ-Đurić, Sanja

(2018)

TY  - CONF
AU  - Vukadinović, Aleksandar
AU  - Janković, Drina
AU  - Mirković, M
AU  - Radović, Magdalena
AU  - Perić, Marko R.
AU  - Milanović, Zorana
AU  - Stanković, D
AU  - Vranješ-Đurić, Sanja
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8068
C3  - European Journal of Nuclear Medicine and Molecular Imaging
T1  - Preparation and radiolabeling of surface-modified magnetic nanoparticles with technetium-99m as potential radiopharmaceuticals in nuclear medicine
VL  - 45
IS  - Supp. 1
SP  - S654
ER  - 
@conference{
author = "Vukadinović, Aleksandar and Janković, Drina and Mirković, M and Radović, Magdalena and Perić, Marko R. and Milanović, Zorana and Stanković, D and Vranješ-Đurić, Sanja",
year = "2018",
journal = "European Journal of Nuclear Medicine and Molecular Imaging",
title = "Preparation and radiolabeling of surface-modified magnetic nanoparticles with technetium-99m as potential radiopharmaceuticals in nuclear medicine",
volume = "45",
number = "Supp. 1",
pages = "S654"
}
Vukadinović, A., Janković, D., Mirković, M., Radović, M., Perić, M. R., Milanović, Z., Stanković, D.,& Vranješ-Đurić, S.. (2018). Preparation and radiolabeling of surface-modified magnetic nanoparticles with technetium-99m as potential radiopharmaceuticals in nuclear medicine. in European Journal of Nuclear Medicine and Molecular Imaging, 45(Supp. 1), S654.
Vukadinović A, Janković D, Mirković M, Radović M, Perić MR, Milanović Z, Stanković D, Vranješ-Đurić S. Preparation and radiolabeling of surface-modified magnetic nanoparticles with technetium-99m as potential radiopharmaceuticals in nuclear medicine. in European Journal of Nuclear Medicine and Molecular Imaging. 2018;45(Supp. 1):S654..
Vukadinović, Aleksandar, Janković, Drina, Mirković, M, Radović, Magdalena, Perić, Marko R., Milanović, Zorana, Stanković, D, Vranješ-Đurić, Sanja, "Preparation and radiolabeling of surface-modified magnetic nanoparticles with technetium-99m as potential radiopharmaceuticals in nuclear medicine" in European Journal of Nuclear Medicine and Molecular Imaging, 45, no. Supp. 1 (2018):S654.