TY  - JOUR
AU  - Stepanović, Aleksandar
AU  - Nikitović, Marina
AU  - Stanojković, Tatjana P.
AU  - Grujičić, Danica
AU  - Bukumirić, Zoran
AU  - Srbljak, Ivana
AU  - Ilić, Rosanda
AU  - Milošević, Snežana
AU  - Arsenijević, Tatjana
AU  - Petrović, Nina
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10265
AB  - A personalized approach to chemoradiation is important in reducing its potential side effects and identifying a group of patients prone to toxicity. MicroRNAs have been shown to have a predictive potential for radiotoxicity. The goal of the study was to test if levels of miRNA in peripheral blood mononuclear cells of glioblastoma patients are associated with toxicity and to identify the peak time point for toxicity. MicroRNA-10b/21/34a levels were measured in 43 patients with and without toxicity, at baseline, at the 15th, and at the 30th fraction by Real-Time quantitative Polymerase Chain Reaction. MicroRNA-10b/21 levels increased with toxicity grade (p = 0.014; p = 0.013); miR-21/34a levels were significantly different between patients with and without toxicity at the 15th fraction (p = 0.030; p = 0.045), while miR-34a levels significantly changed during treatment (p < 0.001). All three miRNAs showed a significantly high positive correlation with one another. MiR-34a might be considered as a predictive factor for toxicity due to its changes during treatment, and differences between the groups with and without toxicity; miR-10b might be used to predict toxicity; miR-10b/21 might be used for predicting the grade of toxicity in GB patients.
T2  - Scientific Reports
T1  - Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide
VL  - 12
IS  - 1
SP  - 7505
DO  - 10.1038/s41598-022-11445-9
ER  - 

@article{
author = "Stepanović, Aleksandar and Nikitović, Marina and Stanojković, Tatjana P. and Grujičić, Danica and Bukumirić, Zoran and Srbljak, Ivana and Ilić, Rosanda and Milošević, Snežana and Arsenijević, Tatjana and Petrović, Nina",
year = "2022",
abstract = "A personalized approach to chemoradiation is important in reducing its potential side effects and identifying a group of patients prone to toxicity. MicroRNAs have been shown to have a predictive potential for radiotoxicity. The goal of the study was to test if levels of miRNA in peripheral blood mononuclear cells of glioblastoma patients are associated with toxicity and to identify the peak time point for toxicity. MicroRNA-10b/21/34a levels were measured in 43 patients with and without toxicity, at baseline, at the 15th, and at the 30th fraction by Real-Time quantitative Polymerase Chain Reaction. MicroRNA-10b/21 levels increased with toxicity grade (p = 0.014; p = 0.013); miR-21/34a levels were significantly different between patients with and without toxicity at the 15th fraction (p = 0.030; p = 0.045), while miR-34a levels significantly changed during treatment (p < 0.001). All three miRNAs showed a significantly high positive correlation with one another. MiR-34a might be considered as a predictive factor for toxicity due to its changes during treatment, and differences between the groups with and without toxicity; miR-10b might be used to predict toxicity; miR-10b/21 might be used for predicting the grade of toxicity in GB patients.",
journal = "Scientific Reports",
title = "Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide",
volume = "12",
number = "1",
pages = "7505",
doi = "10.1038/s41598-022-11445-9"
}

Stepanović, A., Nikitović, M., Stanojković, T. P., Grujičić, D., Bukumirić, Z., Srbljak, I., Ilić, R., Milošević, S., Arsenijević, T.,& Petrović, N.. (2022). Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide. in Scientific Reports, 12(1), 7505.
https://doi.org/10.1038/s41598-022-11445-9

Stepanović A, Nikitović M, Stanojković TP, Grujičić D, Bukumirić Z, Srbljak I, Ilić R, Milošević S, Arsenijević T, Petrović N. Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide. in Scientific Reports. 2022;12(1):7505.
doi:10.1038/s41598-022-11445-9 .

Stepanović, Aleksandar, Nikitović, Marina, Stanojković, Tatjana P., Grujičić, Danica, Bukumirić, Zoran, Srbljak, Ivana, Ilić, Rosanda, Milošević, Snežana, Arsenijević, Tatjana, Petrović, Nina, "Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide" in Scientific Reports, 12, no. 1 (2022):7505,
https://doi.org/10.1038/s41598-022-11445-9 . .

TY  - JOUR
AU  - Mališić, Emina
AU  - Petrović, Nina
AU  - Brengues, Muriel
AU  - Azria, David
AU  - Matić, Ivana Z.
AU  - Srbljak Ćuk, Ivana
AU  - Kopčalić, Katarina
AU  - Stanojković, Tatjana P.
AU  - Nikitović, Marina
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10544
AB  - The genetic background of each person might affect the severity of radiotherapy (RT)-induced normal tissue toxicity. The aim of study was to evaluate the influence of TGFB1 C-509T and Leu10Pro, XRCC1 Arg280His and XRCC3 Thr241Met polymorphisms as well as the level of radiation-induced CD8 T-lymphocyte apoptosis (RILA) on adverse effects of RT for prostate cancer (PCa). The study included 88 patients with localized or locally advanced PCa who were treated with RT. The polymorphisms were determined by PCR–RFLP analysis on DNA from peripheral blood mononuclear cells. RILA values were measured by flow cytometry. We found that CT genotype of TGFB1 C-509T could be protective biomarker for acute genitourinary (GU) and gastrointestinal (GI) radiotoxicity, while Thr variant of XRCC3 Thr241Met could predict the risk for acute GU radiotoxicity. Correlation between RILA values and toxicity was not detected. Univariate logistic regression analysis showed that Gleason score and risk group were risk factors for late GU, while for late GI radiotoxicity it was diabetes mellitus type 2. However, in multivariate model those were not proven to be significant and independent risk factors. Identification of assays combination predicting individual radiosensitivity is a crucial step towards personalized RT approach.
T2  - Scientific Reports
T1  - Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer
VL  - 12
IS  - 1
SP  - 21306
DO  - 10.1038/s41598-022-25328-6
ER  - 

@article{
author = "Mališić, Emina and Petrović, Nina and Brengues, Muriel and Azria, David and Matić, Ivana Z. and Srbljak Ćuk, Ivana and Kopčalić, Katarina and Stanojković, Tatjana P. and Nikitović, Marina",
year = "2022",
abstract = "The genetic background of each person might affect the severity of radiotherapy (RT)-induced normal tissue toxicity. The aim of study was to evaluate the influence of TGFB1 C-509T and Leu10Pro, XRCC1 Arg280His and XRCC3 Thr241Met polymorphisms as well as the level of radiation-induced CD8 T-lymphocyte apoptosis (RILA) on adverse effects of RT for prostate cancer (PCa). The study included 88 patients with localized or locally advanced PCa who were treated with RT. The polymorphisms were determined by PCR–RFLP analysis on DNA from peripheral blood mononuclear cells. RILA values were measured by flow cytometry. We found that CT genotype of TGFB1 C-509T could be protective biomarker for acute genitourinary (GU) and gastrointestinal (GI) radiotoxicity, while Thr variant of XRCC3 Thr241Met could predict the risk for acute GU radiotoxicity. Correlation between RILA values and toxicity was not detected. Univariate logistic regression analysis showed that Gleason score and risk group were risk factors for late GU, while for late GI radiotoxicity it was diabetes mellitus type 2. However, in multivariate model those were not proven to be significant and independent risk factors. Identification of assays combination predicting individual radiosensitivity is a crucial step towards personalized RT approach.",
journal = "Scientific Reports",
title = "Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer",
volume = "12",
number = "1",
pages = "21306",
doi = "10.1038/s41598-022-25328-6"
}

Mališić, E., Petrović, N., Brengues, M., Azria, D., Matić, I. Z., Srbljak Ćuk, I., Kopčalić, K., Stanojković, T. P.,& Nikitović, M.. (2022). Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer. in Scientific Reports, 12(1), 21306.
https://doi.org/10.1038/s41598-022-25328-6

Mališić E, Petrović N, Brengues M, Azria D, Matić IZ, Srbljak Ćuk I, Kopčalić K, Stanojković TP, Nikitović M. Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer. in Scientific Reports. 2022;12(1):21306.
doi:10.1038/s41598-022-25328-6 .

Mališić, Emina, Petrović, Nina, Brengues, Muriel, Azria, David, Matić, Ivana Z., Srbljak Ćuk, Ivana, Kopčalić, Katarina, Stanojković, Tatjana P., Nikitović, Marina, "Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer" in Scientific Reports, 12, no. 1 (2022):21306,
https://doi.org/10.1038/s41598-022-25328-6 . .

TY  - CONF
AU  - Mališić, Emina
AU  - Petrović, Nina
AU  - Nikitović, Marina
PY  - 2022
PY  - S1
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12422
AB  - Purpose or Objective About half of all prostate cancer (PCa) patients receive radiotherapy (RT) either as single curative treatment or as adjuvant/salvage treatment after radical prostatectomy. However, RT is associated with a spectrum of side effects (toxicity) in the surrounding normal tissues. Acute toxicity occurs within 90 days of treatment, is usually transient and affects skin and mucosa of bladder and intestine resulting in dermatitis, cystitis or diarrhea. XRCC3 gene encodes for protein that is involved in homologous recombination repair of double-strand breaks created by ionizing radiation. Disruption of these pathways has the potential to affect the normal tissue response to RT. The T variant of XRCC3 Thr241Met single nucleotide polymorphisms (SNP) in exon 7 (C>T, rs861539) was reported to be associated with elevated levels of DNA adducts, chromosomal deletions, sensitivity to ionizing radiation and cross-linking agents. The aim of this study was to examine association between this SNP and RT-induced normal tissue toxicity in PCa patients. Materials and Methods Eighty one patients who had a histologically confirmed localized or locally advanced PCa were included in the study. Patients were treated with 3DC RT (n=70) or ARC RT (n=11) with radical (72 Gy)(47 patients) or postoperative/salvage (66 Gy)(34 patients) RT without previous hormonal therapy. DNA from peripheral blood mononuclear cells was extracted by salting out method. XRCC3 Thr241Met SNP was determined by PCR-RFLP analysis. The restriction fragments were separated on 2100 Bioanalyzer using DNA 1000 kit. The differences in the distribution of genotypes of XRCC3 Thr241Met between patients with or without acute RT-induced genitourinary (GU) or gastrointestinal (GI) toxicity were tested by χ2 and Fisher’s exact test. P values ≤ 0.05 were considered statistically significant, while p values between 0.1 and 0.05 were pointed out as a statistical trend. Data were analyzed by SigmaStat 3.5. Results The acute GI toxicity appeared in 100%, 94.6% and 81.8% of ThrThr, ThrMet and MetMet PCa patients, raspectivelly. There was the statistical trend towards higher acute GU toxicity in carriers of Thr variant (ThrThr plus ThrMet) vs. MetMet (p=0.087) as well as ThrThr vs. MetMet (p=0.058). For acute GI toxicity, there was a similar distribution in genotypes: 90.9%, 91.9%, 90.9% for ThrThr, ThrMet, MetMet, respectively. PCa patients with ThrThr genotype had higher rate of acute GU toxicity grade ≥2 (45.5%) than ThrMet (28.6%) and MetMet (22.2%) while in GI toxicity MetMet had higher rate of grade ≥2 (40%) than ThrThr (23.3%) and ThrMet (23.5%) but without statistical significance. Conclusion The obtained data indicate that Thr variant of XRCC3 Thr241Met SNP is related to acute GU toxicity. Grade ≥2 acute GU toxicity could be associated with ThrThr while GI toxicity with MetMet genotype. Further study on larger group is necessary to confirm this date and to clarify mechanism underlying this observation.
PB  - Elsevier
C3  - ESTRO 2022 : Radiotherapy & Oncology : Journal of the European SocieTy for Radiotherapy and Oncology : Book of abstracts
T1  - XRCC3 Thr241Met gene polymorphism and acute radiotherapy induced toxicity for prostate cancer
VL  - 170
SP  - S1621
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12422
ER  - 

@conference{
author = "Mališić, Emina and Petrović, Nina and Nikitović, Marina",
year = "2022, S1",
abstract = "Purpose or Objective About half of all prostate cancer (PCa) patients receive radiotherapy (RT) either as single curative treatment or as adjuvant/salvage treatment after radical prostatectomy. However, RT is associated with a spectrum of side effects (toxicity) in the surrounding normal tissues. Acute toxicity occurs within 90 days of treatment, is usually transient and affects skin and mucosa of bladder and intestine resulting in dermatitis, cystitis or diarrhea. XRCC3 gene encodes for protein that is involved in homologous recombination repair of double-strand breaks created by ionizing radiation. Disruption of these pathways has the potential to affect the normal tissue response to RT. The T variant of XRCC3 Thr241Met single nucleotide polymorphisms (SNP) in exon 7 (C>T, rs861539) was reported to be associated with elevated levels of DNA adducts, chromosomal deletions, sensitivity to ionizing radiation and cross-linking agents. The aim of this study was to examine association between this SNP and RT-induced normal tissue toxicity in PCa patients. Materials and Methods Eighty one patients who had a histologically confirmed localized or locally advanced PCa were included in the study. Patients were treated with 3DC RT (n=70) or ARC RT (n=11) with radical (72 Gy)(47 patients) or postoperative/salvage (66 Gy)(34 patients) RT without previous hormonal therapy. DNA from peripheral blood mononuclear cells was extracted by salting out method. XRCC3 Thr241Met SNP was determined by PCR-RFLP analysis. The restriction fragments were separated on 2100 Bioanalyzer using DNA 1000 kit. The differences in the distribution of genotypes of XRCC3 Thr241Met between patients with or without acute RT-induced genitourinary (GU) or gastrointestinal (GI) toxicity were tested by χ2 and Fisher’s exact test. P values ≤ 0.05 were considered statistically significant, while p values between 0.1 and 0.05 were pointed out as a statistical trend. Data were analyzed by SigmaStat 3.5. Results The acute GI toxicity appeared in 100%, 94.6% and 81.8% of ThrThr, ThrMet and MetMet PCa patients, raspectivelly. There was the statistical trend towards higher acute GU toxicity in carriers of Thr variant (ThrThr plus ThrMet) vs. MetMet (p=0.087) as well as ThrThr vs. MetMet (p=0.058). For acute GI toxicity, there was a similar distribution in genotypes: 90.9%, 91.9%, 90.9% for ThrThr, ThrMet, MetMet, respectively. PCa patients with ThrThr genotype had higher rate of acute GU toxicity grade ≥2 (45.5%) than ThrMet (28.6%) and MetMet (22.2%) while in GI toxicity MetMet had higher rate of grade ≥2 (40%) than ThrThr (23.3%) and ThrMet (23.5%) but without statistical significance. Conclusion The obtained data indicate that Thr variant of XRCC3 Thr241Met SNP is related to acute GU toxicity. Grade ≥2 acute GU toxicity could be associated with ThrThr while GI toxicity with MetMet genotype. Further study on larger group is necessary to confirm this date and to clarify mechanism underlying this observation.",
publisher = "Elsevier",
journal = "ESTRO 2022 : Radiotherapy & Oncology : Journal of the European SocieTy for Radiotherapy and Oncology : Book of abstracts",
title = "XRCC3 Thr241Met gene polymorphism and acute radiotherapy induced toxicity for prostate cancer",
volume = "170",
pages = "S1621",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12422"
}

Mališić, E., Petrović, N.,& Nikitović, M.. (2022). XRCC3 Thr241Met gene polymorphism and acute radiotherapy induced toxicity for prostate cancer. in ESTRO 2022 : Radiotherapy & Oncology : Journal of the European SocieTy for Radiotherapy and Oncology : Book of abstracts
Elsevier., 170, S1621.
https://hdl.handle.net/21.15107/rcub_vinar_12422

Mališić E, Petrović N, Nikitović M. XRCC3 Thr241Met gene polymorphism and acute radiotherapy induced toxicity for prostate cancer. in ESTRO 2022 : Radiotherapy & Oncology : Journal of the European SocieTy for Radiotherapy and Oncology : Book of abstracts. 2022;170:S1621.
https://hdl.handle.net/21.15107/rcub_vinar_12422 .

Mališić, Emina, Petrović, Nina, Nikitović, Marina, "XRCC3 Thr241Met gene polymorphism and acute radiotherapy induced toxicity for prostate cancer" in ESTRO 2022 : Radiotherapy & Oncology : Journal of the European SocieTy for Radiotherapy and Oncology : Book of abstracts, 170 (2022):S1621,
https://hdl.handle.net/21.15107/rcub_vinar_12422 .

TY  - CONF
AU  - Stepanović, Aleksandar
AU  - Petrović, Nina
AU  - Ilić, Rosanda
AU  - Bogdanović, Ivan
AU  - Arsenijević, Tatjana
AU  - Grujičić, Danica
AU  - Nikitović, Marina
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12458
C3  - Annals of cancerology section : 59th Cancerology Week : Book of asbtracts
T1  - MikroRNK kao prediktori radiotoksičnosti kod pacijenata sa glioblastomom
SP  - 114
EP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12458
ER  - 

@conference{
author = "Stepanović, Aleksandar and Petrović, Nina and Ilić, Rosanda and Bogdanović, Ivan and Arsenijević, Tatjana and Grujičić, Danica and Nikitović, Marina",
year = "2022",
journal = "Annals of cancerology section : 59th Cancerology Week : Book of asbtracts",
title = "MikroRNK kao prediktori radiotoksičnosti kod pacijenata sa glioblastomom",
pages = "114-115",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12458"
}

Stepanović, A., Petrović, N., Ilić, R., Bogdanović, I., Arsenijević, T., Grujičić, D.,& Nikitović, M.. (2022). MikroRNK kao prediktori radiotoksičnosti kod pacijenata sa glioblastomom. in Annals of cancerology section : 59th Cancerology Week : Book of asbtracts, 114-115.
https://hdl.handle.net/21.15107/rcub_vinar_12458

Stepanović A, Petrović N, Ilić R, Bogdanović I, Arsenijević T, Grujičić D, Nikitović M. MikroRNK kao prediktori radiotoksičnosti kod pacijenata sa glioblastomom. in Annals of cancerology section : 59th Cancerology Week : Book of asbtracts. 2022;:114-115.
https://hdl.handle.net/21.15107/rcub_vinar_12458 .

Stepanović, Aleksandar, Petrović, Nina, Ilić, Rosanda, Bogdanović, Ivan, Arsenijević, Tatjana, Grujičić, Danica, Nikitović, Marina, "MikroRNK kao prediktori radiotoksičnosti kod pacijenata sa glioblastomom" in Annals of cancerology section : 59th Cancerology Week : Book of asbtracts (2022):114-115,
https://hdl.handle.net/21.15107/rcub_vinar_12458 .

TY  - CONF
AU  - Stepanović, Aleksandar
AU  - Petrović, Nina
AU  - Arsenijević, Tatjana
AU  - Nikitović, Marina
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12412
AB  - Background: Standard treatment of the glioblastoma patients is implemented with Stupp’s protocol since 2005, but 2-years survival of the patients are still at low percentage. Even with implementation of modern radiotherapy techniques, acute radiotoxicity is still observed in these patients. Micro RNAs (miRNAs) expression patterns are introduced in recent years as factors that might predict survival in patients with glioblastoma, as well as cancer treatment related side effects. Material and methods: The data used in this review were conducted from research papers in PUBMED/MEDLINE databases with a special focus on role of miRNAs in cancer and with emphasis on brain tumors correlated to acute radiotoxicity. Results: Inflammation due to oxidative stress via free radicals is one of the mechanisms of radiation injury of irradiated cells and may correlate with toxicity. MiRNAs expression in prostate cancer patients may predict acute radiotoxicity, while miRNAs expression levels that could predict radiation toxicity in glioblastoma are still under investigation. However, it has been shown that miRNA levels like miR-16, miR 21, miR-19a and miR-22 are increased after radiation in glioma, while levels of miR-107, miR-181a are decreased. Conclusion: Recent studies have shown that miRNAs may have role as potent indicator of radiotoxicity in cancer patients. To date, there is no available data about acute brain radiotoxicity and its correlation with expression levels of mRNAs in patients with GB. There is a emerge need for further investigation on role of radioresponsive miRNAs in glioblastoma patients with acute radiotoxicity.
PB  - Belgrade : Serbian Association for Cancer Research (SDIR)
C3  - SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts
T1  - Can miRNA expression patterns predict radiotoxicity in patients with glioblastoma?
SP  - 19
EP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12412
ER  - 

@conference{
author = "Stepanović, Aleksandar and Petrović, Nina and Arsenijević, Tatjana and Nikitović, Marina",
year = "2021",
abstract = "Background: Standard treatment of the glioblastoma patients is implemented with Stupp’s protocol since 2005, but 2-years survival of the patients are still at low percentage. Even with implementation of modern radiotherapy techniques, acute radiotoxicity is still observed in these patients. Micro RNAs (miRNAs) expression patterns are introduced in recent years as factors that might predict survival in patients with glioblastoma, as well as cancer treatment related side effects. Material and methods: The data used in this review were conducted from research papers in PUBMED/MEDLINE databases with a special focus on role of miRNAs in cancer and with emphasis on brain tumors correlated to acute radiotoxicity. Results: Inflammation due to oxidative stress via free radicals is one of the mechanisms of radiation injury of irradiated cells and may correlate with toxicity. MiRNAs expression in prostate cancer patients may predict acute radiotoxicity, while miRNAs expression levels that could predict radiation toxicity in glioblastoma are still under investigation. However, it has been shown that miRNA levels like miR-16, miR 21, miR-19a and miR-22 are increased after radiation in glioma, while levels of miR-107, miR-181a are decreased. Conclusion: Recent studies have shown that miRNAs may have role as potent indicator of radiotoxicity in cancer patients. To date, there is no available data about acute brain radiotoxicity and its correlation with expression levels of mRNAs in patients with GB. There is a emerge need for further investigation on role of radioresponsive miRNAs in glioblastoma patients with acute radiotoxicity.",
publisher = "Belgrade : Serbian Association for Cancer Research (SDIR)",
journal = "SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts",
title = "Can miRNA expression patterns predict radiotoxicity in patients with glioblastoma?",
pages = "19-19",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12412"
}

Stepanović, A., Petrović, N., Arsenijević, T.,& Nikitović, M.. (2021). Can miRNA expression patterns predict radiotoxicity in patients with glioblastoma?. in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts
Belgrade : Serbian Association for Cancer Research (SDIR)., 19-19.
https://hdl.handle.net/21.15107/rcub_vinar_12412

Stepanović A, Petrović N, Arsenijević T, Nikitović M. Can miRNA expression patterns predict radiotoxicity in patients with glioblastoma?. in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts. 2021;:19-19.
https://hdl.handle.net/21.15107/rcub_vinar_12412 .

Stepanović, Aleksandar, Petrović, Nina, Arsenijević, Tatjana, Nikitović, Marina, "Can miRNA expression patterns predict radiotoxicity in patients with glioblastoma?" in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts (2021):19-19,
https://hdl.handle.net/21.15107/rcub_vinar_12412 .

TY  - CONF
AU  - Mališić, Emina
AU  - Petrović, Nina
AU  - Nikitović, Marina
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12414
AB  - Background: Radiotherapy (RT)-induced acute toxicity associated with bladder and bowel injury has great impact the quality of life for prostate cancer (PCa) patients. TGFB1 is a key proinflammatory and profibrotic cytokine, but its role in acute toxicity is still unclear. TGFB1 T>C transition at codon 10 results in leucine to proline substitution and increased TGFB1 protein levels. The aim of this study was to examine impact of TGFB1 Leu10Pro (rs1800470) polymorphism on RT-induced acute toxicity in PCa patients. Patients and methods: Eighty two patients who had a localized or locally advanced PCa were treated with radical (72 Gy)(n=47) or postoperative/salvage (66 Gy)(n=35) RT without previous hormonal therapy. TGFB1 Leu10Pro was determined by PCR-RFLP analysis on DNA from PBMC. The differences in the distribution of genotypes for dominant, recessive, codominant and overdominant genetic model between patients with or without acute genitourinary (GU) or gastrointestinal (GI) toxicity as well as different grade of toxicity were tested by χ2 and Fisher’s exact test. P values ≤0.05 were considered statistically significant. Results: Heterozygote PCa patients had lower rate of acute GU and GI toxicity then homozygotes (LeuLeu, LeuPro, ProPro were: 100%, 90.7%, 100% for GU and 92.0%, 88.4%, 100%, respectively for GI). The frequency of toxicity grade ≥2 were higher in LeuPro then both homozygote carriers (41% vs. 28.2% for GU and 26.3% vs. 21.6% for GI acute toxicity). The differences were not statistically significant. Conclusion: The present study did not establish impact of TGFB1 Leu10Pro polymorphism on RT-induced acute toxicity in PCa patients.
PB  - Belgrade : Serbian Association for Cancer Research (SDIR)
C3  - SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts
T1  - Impact of TGFB1 Leu10Pro polymorphism on acute radiotherapy-induced toxicity in prostate cancer patients
SP  - 61
EP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12414
ER  - 

@conference{
author = "Mališić, Emina and Petrović, Nina and Nikitović, Marina",
year = "2021",
abstract = "Background: Radiotherapy (RT)-induced acute toxicity associated with bladder and bowel injury has great impact the quality of life for prostate cancer (PCa) patients. TGFB1 is a key proinflammatory and profibrotic cytokine, but its role in acute toxicity is still unclear. TGFB1 T>C transition at codon 10 results in leucine to proline substitution and increased TGFB1 protein levels. The aim of this study was to examine impact of TGFB1 Leu10Pro (rs1800470) polymorphism on RT-induced acute toxicity in PCa patients. Patients and methods: Eighty two patients who had a localized or locally advanced PCa were treated with radical (72 Gy)(n=47) or postoperative/salvage (66 Gy)(n=35) RT without previous hormonal therapy. TGFB1 Leu10Pro was determined by PCR-RFLP analysis on DNA from PBMC. The differences in the distribution of genotypes for dominant, recessive, codominant and overdominant genetic model between patients with or without acute genitourinary (GU) or gastrointestinal (GI) toxicity as well as different grade of toxicity were tested by χ2 and Fisher’s exact test. P values ≤0.05 were considered statistically significant. Results: Heterozygote PCa patients had lower rate of acute GU and GI toxicity then homozygotes (LeuLeu, LeuPro, ProPro were: 100%, 90.7%, 100% for GU and 92.0%, 88.4%, 100%, respectively for GI). The frequency of toxicity grade ≥2 were higher in LeuPro then both homozygote carriers (41% vs. 28.2% for GU and 26.3% vs. 21.6% for GI acute toxicity). The differences were not statistically significant. Conclusion: The present study did not establish impact of TGFB1 Leu10Pro polymorphism on RT-induced acute toxicity in PCa patients.",
publisher = "Belgrade : Serbian Association for Cancer Research (SDIR)",
journal = "SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts",
title = "Impact of TGFB1 Leu10Pro polymorphism on acute radiotherapy-induced toxicity in prostate cancer patients",
pages = "61-61",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12414"
}

Mališić, E., Petrović, N.,& Nikitović, M.. (2021). Impact of TGFB1 Leu10Pro polymorphism on acute radiotherapy-induced toxicity in prostate cancer patients. in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts
Belgrade : Serbian Association for Cancer Research (SDIR)., 61-61.
https://hdl.handle.net/21.15107/rcub_vinar_12414

Mališić E, Petrović N, Nikitović M. Impact of TGFB1 Leu10Pro polymorphism on acute radiotherapy-induced toxicity in prostate cancer patients. in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts. 2021;:61-61.
https://hdl.handle.net/21.15107/rcub_vinar_12414 .

Mališić, Emina, Petrović, Nina, Nikitović, Marina, "Impact of TGFB1 Leu10Pro polymorphism on acute radiotherapy-induced toxicity in prostate cancer patients" in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts (2021):61-61,
https://hdl.handle.net/21.15107/rcub_vinar_12414 .

TY  - JOUR
AU  - Stanojković, Tatjana P.
AU  - Matić, Ivana Z.
AU  - Petrović, Nina
AU  - Stanković, Vesna
AU  - Kopčalić, Katarina
AU  - Besu, Irina
AU  - Đorđić Crnogorac, Marija
AU  - Mališić, Emina
AU  - Mirjačić-Martinović, Katarina
AU  - Vuletić, Ana
AU  - Bukumirić, Zoran
AU  - Žižak, Željko
AU  - Veldwijk, Marlon
AU  - Herskind, Carsten
AU  - Nikitović, Marina
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9713
AB  - One of the challenges of radiation oncology in the era of personalized medicine is identification of biomarkers associated with individual radiosensitivity. The aim of research was to evaluate the possible clinical value of the associations between clinical, physical, and biological factors, and risk for development of acute radiotoxicity in patients with prostate cancer. The study involved forty four patients treated with three-dimensional conformal radiotherapy. The concentrations of IL-1β, IL-2, IL-6, IFN-γ and TGF-β1 were assessed before radiotherapy, after 5th, 15th and 25th radiotherapy fractions, at the end, and 1 month after the end of radiotherapy. Cytokine gene expression was determined in peripheral blood mononuclear cells. The univariate analysis of circulating cytokine levels during radiotherapy showed that increased serum concentrations of IL-6 were significantly associated with higher grade of acute genitourinary toxicity. The multivariate analysis demonstrated that increased level of IL-6 during the radiotherapy was significantly associated with higher grade of acute genitourinary toxicity across treatment. TGF-β expression levels significantly decreased during course of radiotherapy. Research indicates that changes in circulating cytokine levels might be important parameter of radiotoxicity in patients with prostate cancer. These findings suggest that future studies based on multi-parameter examination are necessary for prediction of individual radiosensitivity.
T2  - Scientific Reports
T1  - Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients
VL  - 10
IS  - 1
SP  - 19002
DO  - 10.1038/s41598-020-75812-0
ER  - 

@article{
author = "Stanojković, Tatjana P. and Matić, Ivana Z. and Petrović, Nina and Stanković, Vesna and Kopčalić, Katarina and Besu, Irina and Đorđić Crnogorac, Marija and Mališić, Emina and Mirjačić-Martinović, Katarina and Vuletić, Ana and Bukumirić, Zoran and Žižak, Željko and Veldwijk, Marlon and Herskind, Carsten and Nikitović, Marina",
year = "2020",
abstract = "One of the challenges of radiation oncology in the era of personalized medicine is identification of biomarkers associated with individual radiosensitivity. The aim of research was to evaluate the possible clinical value of the associations between clinical, physical, and biological factors, and risk for development of acute radiotoxicity in patients with prostate cancer. The study involved forty four patients treated with three-dimensional conformal radiotherapy. The concentrations of IL-1β, IL-2, IL-6, IFN-γ and TGF-β1 were assessed before radiotherapy, after 5th, 15th and 25th radiotherapy fractions, at the end, and 1 month after the end of radiotherapy. Cytokine gene expression was determined in peripheral blood mononuclear cells. The univariate analysis of circulating cytokine levels during radiotherapy showed that increased serum concentrations of IL-6 were significantly associated with higher grade of acute genitourinary toxicity. The multivariate analysis demonstrated that increased level of IL-6 during the radiotherapy was significantly associated with higher grade of acute genitourinary toxicity across treatment. TGF-β expression levels significantly decreased during course of radiotherapy. Research indicates that changes in circulating cytokine levels might be important parameter of radiotoxicity in patients with prostate cancer. These findings suggest that future studies based on multi-parameter examination are necessary for prediction of individual radiosensitivity.",
journal = "Scientific Reports",
title = "Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients",
volume = "10",
number = "1",
pages = "19002",
doi = "10.1038/s41598-020-75812-0"
}

Stanojković, T. P., Matić, I. Z., Petrović, N., Stanković, V., Kopčalić, K., Besu, I., Đorđić Crnogorac, M., Mališić, E., Mirjačić-Martinović, K., Vuletić, A., Bukumirić, Z., Žižak, Ž., Veldwijk, M., Herskind, C.,& Nikitović, M.. (2020). Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients. in Scientific Reports, 10(1), 19002.
https://doi.org/10.1038/s41598-020-75812-0

Stanojković TP, Matić IZ, Petrović N, Stanković V, Kopčalić K, Besu I, Đorđić Crnogorac M, Mališić E, Mirjačić-Martinović K, Vuletić A, Bukumirić Z, Žižak Ž, Veldwijk M, Herskind C, Nikitović M. Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients. in Scientific Reports. 2020;10(1):19002.
doi:10.1038/s41598-020-75812-0 .

Stanojković, Tatjana P., Matić, Ivana Z., Petrović, Nina, Stanković, Vesna, Kopčalić, Katarina, Besu, Irina, Đorđić Crnogorac, Marija, Mališić, Emina, Mirjačić-Martinović, Katarina, Vuletić, Ana, Bukumirić, Zoran, Žižak, Željko, Veldwijk, Marlon, Herskind, Carsten, Nikitović, Marina, "Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients" in Scientific Reports, 10, no. 1 (2020):19002,
https://doi.org/10.1038/s41598-020-75812-0 . .

TY  - JOUR
AU  - Kopčalić, Katarina
AU  - Petrović, Nina
AU  - Stanojković, Tatjana P.
AU  - Stanković, Vesna
AU  - Bukumirić, Zoran
AU  - Roganović, Jelena
AU  - Mališić, Emina
AU  - Nikitović, Marina
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8444
AB  - Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative ΔΔCt method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs. © 2018 Elsevier GmbH
T2  - Pathology - Research and Practice
T1  - Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study
VL  - 215
IS  - 4
SP  - 626
EP  - 631
DO  - 10.1016/j.prp.2018.12.007
ER  - 

@article{
author = "Kopčalić, Katarina and Petrović, Nina and Stanojković, Tatjana P. and Stanković, Vesna and Bukumirić, Zoran and Roganović, Jelena and Mališić, Emina and Nikitović, Marina",
year = "2019",
abstract = "Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative ΔΔCt method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs. © 2018 Elsevier GmbH",
journal = "Pathology - Research and Practice",
title = "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study",
volume = "215",
number = "4",
pages = "626-631",
doi = "10.1016/j.prp.2018.12.007"
}

Kopčalić, K., Petrović, N., Stanojković, T. P., Stanković, V., Bukumirić, Z., Roganović, J., Mališić, E.,& Nikitović, M.. (2019). Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology - Research and Practice, 215(4), 626-631.
https://doi.org/10.1016/j.prp.2018.12.007

Kopčalić K, Petrović N, Stanojković TP, Stanković V, Bukumirić Z, Roganović J, Mališić E, Nikitović M. Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology - Research and Practice. 2019;215(4):626-631.
doi:10.1016/j.prp.2018.12.007 .

Kopčalić, Katarina, Petrović, Nina, Stanojković, Tatjana P., Stanković, Vesna, Bukumirić, Zoran, Roganović, Jelena, Mališić, Emina, Nikitović, Marina, "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study" in Pathology - Research and Practice, 215, no. 4 (2019):626-631,
https://doi.org/10.1016/j.prp.2018.12.007 . .