TY  - JOUR
AU  - Arsić, Biljana
AU  - Petrović, Stefan
AU  - Mrmošanin, Jelena
AU  - Dimitrijević, Ivana
AU  - Tošić, Snežana
AU  - Stojanović, Gordana
AU  - Glišić, Sanja
AU  - Milićević, Jelena
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12852
AB  - Pesticides commonly used in the Republic of Serbia (tebuconazole, pendimethalin, pyraclostrobin, propiconazole, and famoxadone) have high stability, so their potential toxicity to humans needs to be investigated. These pesticides are in use in the Republic of Serbia in various formulations. Their toxicity and interactions with acetylcholinesterase were thoroughly investigated in this study using computational tools. The ADMET (Adsorption, Distribution, Metabolism, Excretion, Toxicity) study showed that all of them are good oral compounds, and that pendimethalin was a mutagenic compound. Glide scores ranged from -18.41 kJ mol-1 (pendimethalin) to -27.61 kJ mol-1 (famoxadone) in Mus musculus, and from -19.58 kJ mol-1 (pendimethalin) to -24.31 kJ mol-1 (propiconazole) in Homo sapiens. In addition, the experimental stability of the pesticides solutions in methanol was studied using the fast GC-MS (retention times of the studied pesticides ranged from 14.47 min (pendimethalin) to 22 min (famoxadone)). They showed good stability over time, apart from pyraclostrobin which decomposed mainly into its des-methoxy derivative after 20 months. Based on the promising modeling results, pyraclostrobin and famoxadone emerge as potential candidates for further investigation in the treatment of Alzheimer's disease, taking care to increase their stability.
AB  - Пестициди који се често користе у Републици Србији (тебуконазол, пендиметалин, пираклостробин, пропиконазол, и фамоксадон) имају високу стабилност, тако да њихова потенцијална токсичност се мора проучити. Ови пестициди се користе у Републици Србији у различитим формулацијама. Њихова токсичност и интеракције са ацетихолинестеразом су детаљно изучене у овом истраживању коришћењем компјутерских алата. АДМЕТ (Адсорпција, Дистрибуција, Метаболизам, Екскреција, Токсичност) изучавање је показало да су сви они добра орална једињења, и да је пендиметалин мутагено једињење. Глајд скорови су били у опсегу од -18,41 kJ mol -1 (пендиметалин) до -27,61 kJ mol-1 (фамоксадон) код миша, и од -19,58 kJ mol-1 (пендиметалин) до -24,31 kJ mol-1 (пропиконазол) код човека. Осим тога, експериментална стабилност раствора пестицида у метанолу је проучавана коришћењем ГХ-МС (ретенциона времена проучаваних пестицида се кретала од 14.47 мин (пендиметалин) до 22 мин (фамоксадон)). Они показују добру стабилност током времена, осим пираклостробина који се углавном распада на свој дез-метокси дериват после 20 месеци. На основу обећавајућих резултата моделовања, пираклостробин и фамоксадон се појављују као потенцијални кандидати који се могу даље изучавати као третман за Алцхајмерову болест, водећи рачина да се повећа њихова стабилност.
T2  - Journal of the Serbian Chemical Society
T1  - Stability and computational analyses of selected pesticides in use in the Republic of Serbia
VL  - 89
IS  - 2
SP  - 259
EP  - 274
DO  - 10.2298/JSC230714102A
ER  - 

@article{
author = "Arsić, Biljana and Petrović, Stefan and Mrmošanin, Jelena and Dimitrijević, Ivana and Tošić, Snežana and Stojanović, Gordana and Glišić, Sanja and Milićević, Jelena",
year = "2024",
abstract = "Pesticides commonly used in the Republic of Serbia (tebuconazole, pendimethalin, pyraclostrobin, propiconazole, and famoxadone) have high stability, so their potential toxicity to humans needs to be investigated. These pesticides are in use in the Republic of Serbia in various formulations. Their toxicity and interactions with acetylcholinesterase were thoroughly investigated in this study using computational tools. The ADMET (Adsorption, Distribution, Metabolism, Excretion, Toxicity) study showed that all of them are good oral compounds, and that pendimethalin was a mutagenic compound. Glide scores ranged from -18.41 kJ mol-1 (pendimethalin) to -27.61 kJ mol-1 (famoxadone) in Mus musculus, and from -19.58 kJ mol-1 (pendimethalin) to -24.31 kJ mol-1 (propiconazole) in Homo sapiens. In addition, the experimental stability of the pesticides solutions in methanol was studied using the fast GC-MS (retention times of the studied pesticides ranged from 14.47 min (pendimethalin) to 22 min (famoxadone)). They showed good stability over time, apart from pyraclostrobin which decomposed mainly into its des-methoxy derivative after 20 months. Based on the promising modeling results, pyraclostrobin and famoxadone emerge as potential candidates for further investigation in the treatment of Alzheimer's disease, taking care to increase their stability., Пестициди који се често користе у Републици Србији (тебуконазол, пендиметалин, пираклостробин, пропиконазол, и фамоксадон) имају високу стабилност, тако да њихова потенцијална токсичност се мора проучити. Ови пестициди се користе у Републици Србији у различитим формулацијама. Њихова токсичност и интеракције са ацетихолинестеразом су детаљно изучене у овом истраживању коришћењем компјутерских алата. АДМЕТ (Адсорпција, Дистрибуција, Метаболизам, Екскреција, Токсичност) изучавање је показало да су сви они добра орална једињења, и да је пендиметалин мутагено једињење. Глајд скорови су били у опсегу од -18,41 kJ mol -1 (пендиметалин) до -27,61 kJ mol-1 (фамоксадон) код миша, и од -19,58 kJ mol-1 (пендиметалин) до -24,31 kJ mol-1 (пропиконазол) код човека. Осим тога, експериментална стабилност раствора пестицида у метанолу је проучавана коришћењем ГХ-МС (ретенциона времена проучаваних пестицида се кретала од 14.47 мин (пендиметалин) до 22 мин (фамоксадон)). Они показују добру стабилност током времена, осим пираклостробина који се углавном распада на свој дез-метокси дериват после 20 месеци. На основу обећавајућих резултата моделовања, пираклостробин и фамоксадон се појављују као потенцијални кандидати који се могу даље изучавати као третман за Алцхајмерову болест, водећи рачина да се повећа њихова стабилност.",
journal = "Journal of the Serbian Chemical Society",
title = "Stability and computational analyses of selected pesticides in use in the Republic of Serbia",
volume = "89",
number = "2",
pages = "259-274",
doi = "10.2298/JSC230714102A"
}

Arsić, B., Petrović, S., Mrmošanin, J., Dimitrijević, I., Tošić, S., Stojanović, G., Glišić, S.,& Milićević, J.. (2024). Stability and computational analyses of selected pesticides in use in the Republic of Serbia. in Journal of the Serbian Chemical Society, 89(2), 259-274.
https://doi.org/10.2298/JSC230714102A

Arsić B, Petrović S, Mrmošanin J, Dimitrijević I, Tošić S, Stojanović G, Glišić S, Milićević J. Stability and computational analyses of selected pesticides in use in the Republic of Serbia. in Journal of the Serbian Chemical Society. 2024;89(2):259-274.
doi:10.2298/JSC230714102A .

Arsić, Biljana, Petrović, Stefan, Mrmošanin, Jelena, Dimitrijević, Ivana, Tošić, Snežana, Stojanović, Gordana, Glišić, Sanja, Milićević, Jelena, "Stability and computational analyses of selected pesticides in use in the Republic of Serbia" in Journal of the Serbian Chemical Society, 89, no. 2 (2024):259-274,
https://doi.org/10.2298/JSC230714102A . .

TY  - JOUR
AU  - Zlatanović, Ivana
AU  - Stanković, Miroslava
AU  - Stankov Jovanović, Vesna
AU  - Mitić, Violeta
AU  - Zrnzevic, Ivana
AU  - Đorđević, Aleksandra
AU  - Stojanović, Gordana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1466
AB  - This paper reports for the first time the effect of an acetone extract of Umbilicaria crustulosa on the micronucleus distribution of human lymphocytes, and on the cholinesterase activity and antioxidant activity by the cupric ion reducing antioxidant capacity (CUPRAC) method. Additionally, the total phenolic compounds (TPC) and the antioxidant properties were estimated via DPPH, ABTS and TRP assays. Moreover, the antibacterial activity against two Gram-positive and three Gram-negative bacteria were determined. Acetone extract of U. crustulosa at concentration of 1 and 2 mu g mL(-1) decreased a frequency of micronuclei (MN) by 10.8 and 16.8 %, respectively, acting more or slightly less than the synthetic protector amifostine (AMF, WR-2721, 11.4 %, at concentration of 1 mu g mL(-1)). The tested extract did not inhibit cholinesterase activity nor did it exhibit activity toward the examined bacteria. The extract reduced the concentration of DPPH and ABTS radicals by 88.7 and 96.2 %, respectively. Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.6197+/-0.0166 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 19.7641+/-1.6546 mu g trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 350.4188 +/-14.587 mu g gallic acid equivalents (GAE) per mg of dry extract. The results of the present study showed that U. crustulosa acetone extract is a promising candidate for in vivo experiments considering its antioxidant activity and protective effect on human lymphocytes.
T2  - Journal of the Serbian Chemical Society
T1  - Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract
VL  - 82
IS  - 2
SP  - 141
EP  - 150
DO  - 10.2298/JSC161019014Z
ER  - 

@article{
author = "Zlatanović, Ivana and Stanković, Miroslava and Stankov Jovanović, Vesna and Mitić, Violeta and Zrnzevic, Ivana and Đorđević, Aleksandra and Stojanović, Gordana",
year = "2017",
abstract = "This paper reports for the first time the effect of an acetone extract of Umbilicaria crustulosa on the micronucleus distribution of human lymphocytes, and on the cholinesterase activity and antioxidant activity by the cupric ion reducing antioxidant capacity (CUPRAC) method. Additionally, the total phenolic compounds (TPC) and the antioxidant properties were estimated via DPPH, ABTS and TRP assays. Moreover, the antibacterial activity against two Gram-positive and three Gram-negative bacteria were determined. Acetone extract of U. crustulosa at concentration of 1 and 2 mu g mL(-1) decreased a frequency of micronuclei (MN) by 10.8 and 16.8 %, respectively, acting more or slightly less than the synthetic protector amifostine (AMF, WR-2721, 11.4 %, at concentration of 1 mu g mL(-1)). The tested extract did not inhibit cholinesterase activity nor did it exhibit activity toward the examined bacteria. The extract reduced the concentration of DPPH and ABTS radicals by 88.7 and 96.2 %, respectively. Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.6197+/-0.0166 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 19.7641+/-1.6546 mu g trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 350.4188 +/-14.587 mu g gallic acid equivalents (GAE) per mg of dry extract. The results of the present study showed that U. crustulosa acetone extract is a promising candidate for in vivo experiments considering its antioxidant activity and protective effect on human lymphocytes.",
journal = "Journal of the Serbian Chemical Society",
title = "Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract",
volume = "82",
number = "2",
pages = "141-150",
doi = "10.2298/JSC161019014Z"
}

Zlatanović, I., Stanković, M., Stankov Jovanović, V., Mitić, V., Zrnzevic, I., Đorđević, A.,& Stojanović, G.. (2017). Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract. in Journal of the Serbian Chemical Society, 82(2), 141-150.
https://doi.org/10.2298/JSC161019014Z

Zlatanović I, Stanković M, Stankov Jovanović V, Mitić V, Zrnzevic I, Đorđević A, Stojanović G. Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract. in Journal of the Serbian Chemical Society. 2017;82(2):141-150.
doi:10.2298/JSC161019014Z .

Zlatanović, Ivana, Stanković, Miroslava, Stankov Jovanović, Vesna, Mitić, Violeta, Zrnzevic, Ivana, Đorđević, Aleksandra, Stojanović, Gordana, "Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract" in Journal of the Serbian Chemical Society, 82, no. 2 (2017):141-150,
https://doi.org/10.2298/JSC161019014Z . .

TY  - JOUR
AU  - Zrnzevic, Ivana
AU  - Stanković, Miroslava
AU  - Stankov Jovanović, Vesna
AU  - Mitić, Violeta
AU  - Đorđević, Aleksandra
AU  - Zlatanović, Ivana
AU  - Stojanović, Gordana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1656
AB  - In the present investigation, effects of Ramalina capitata acetone extract on micronucleus distribution on human lymphocytes, on cholinesterase activity and antioxidant activity (by the CUPRAC method) were examined, for the first time as well as its HPLC profile. Additionally, total phenolic compounds (TPC), antioxidant properties (estimated via DPPH, ABTS and TRP assays) and antibacterial activity were determined. The predominant phenolic compounds in this extract were evernic, everninic and obtusatic acids. Acetone extract of R. capitata at concentration of 2 mu g mL(-1) decreased a frequency of micronuclei (MN) for 14.8 %. The extract reduces the concentration of DPPH and ABTS radicals for 21.2 and 36.1 % (respectively). Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.4624 +/- 0.1064 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 6.1176 +/- 0.2964 mu g Trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 670.6376 +/- 66.554 mu g galic acid equivalents (GAE) per mg of dry extract. Tested extract at concentration of 2 mg mL(-1) exhibited inhibition effect (5.2 %) on pooled human serum cholinesterase. The antimicrobial assay showed that acetone extract had inhibition effect towards Gram-positive strains. The results of manifested antioxidant activity, reducing the number of micronuclei in human lymphocytes, and antibacterial activity recommends R. capitata extract for further in vivo studies.
T2  - EXCLI Journal
T1  - Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity
VL  - 16
SP  - 679
EP  - 687
DO  - 10.17179/excli2017-301
ER  - 

@article{
author = "Zrnzevic, Ivana and Stanković, Miroslava and Stankov Jovanović, Vesna and Mitić, Violeta and Đorđević, Aleksandra and Zlatanović, Ivana and Stojanović, Gordana",
year = "2017",
abstract = "In the present investigation, effects of Ramalina capitata acetone extract on micronucleus distribution on human lymphocytes, on cholinesterase activity and antioxidant activity (by the CUPRAC method) were examined, for the first time as well as its HPLC profile. Additionally, total phenolic compounds (TPC), antioxidant properties (estimated via DPPH, ABTS and TRP assays) and antibacterial activity were determined. The predominant phenolic compounds in this extract were evernic, everninic and obtusatic acids. Acetone extract of R. capitata at concentration of 2 mu g mL(-1) decreased a frequency of micronuclei (MN) for 14.8 %. The extract reduces the concentration of DPPH and ABTS radicals for 21.2 and 36.1 % (respectively). Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.4624 +/- 0.1064 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 6.1176 +/- 0.2964 mu g Trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 670.6376 +/- 66.554 mu g galic acid equivalents (GAE) per mg of dry extract. Tested extract at concentration of 2 mg mL(-1) exhibited inhibition effect (5.2 %) on pooled human serum cholinesterase. The antimicrobial assay showed that acetone extract had inhibition effect towards Gram-positive strains. The results of manifested antioxidant activity, reducing the number of micronuclei in human lymphocytes, and antibacterial activity recommends R. capitata extract for further in vivo studies.",
journal = "EXCLI Journal",
title = "Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity",
volume = "16",
pages = "679-687",
doi = "10.17179/excli2017-301"
}

Zrnzevic, I., Stanković, M., Stankov Jovanović, V., Mitić, V., Đorđević, A., Zlatanović, I.,& Stojanović, G.. (2017). Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity. in EXCLI Journal, 16, 679-687.
https://doi.org/10.17179/excli2017-301

Zrnzevic I, Stanković M, Stankov Jovanović V, Mitić V, Đorđević A, Zlatanović I, Stojanović G. Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity. in EXCLI Journal. 2017;16:679-687.
doi:10.17179/excli2017-301 .

Zrnzevic, Ivana, Stanković, Miroslava, Stankov Jovanović, Vesna, Mitić, Violeta, Đorđević, Aleksandra, Zlatanović, Ivana, Stojanović, Gordana, "Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity" in EXCLI Journal, 16 (2017):679-687,
https://doi.org/10.17179/excli2017-301 . .