TY  - JOUR
AU  - Radovanović, Jelena
AU  - Antonijević, Biljana
AU  - Ćurčić, Marijana
AU  - Baralić, Katarina
AU  - Kolarević, Stoimir
AU  - Bulat, Zorica
AU  - Đukić-Ćosić, Danijela
AU  - Buha-Đorđević, Aleksandra
AU  - Vuković-Gačić, Branka
AU  - Javorac, Dragana
AU  - Antonijević Miljaković, Evica
AU  - Carević, Momir
AU  - Mandinić, Zoran
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10451
AB  - Excessive fluoride (F−) levels in the environment could induce different pathological changes, including comorbidities in reproductive functions. Hence, the aim of the present in vivo study was to explore F− subacute toxicity mechanisms via Benchmark dose (BMD) methodology on rat's testicles. The experiment was conducted on thirty male Wistar rats for 28 days, divided into six groups (n = 5): 1) Control (tap water); 2) 10 mg/L F−; 3) 25 mg/L F−; 4) 50 mg/L F−; 5) 100 mg/L F−; 6) 150 mg/L F−. Testicles were dissected out and processed for the determination of F− tissue concentrations, redox status parameters, essential elements level, and DNA damage. PROASTweb 70.1 software was used for determination of external and internal dose-response relationship. The results confirmed a significant increase in superoxide anion (O2.-), total oxidative status (TOS), copper (Cu), zinc (Zn), iron (Fe), DNA damage levels, and decrease in superoxide dismutase activity (SOD1) and total thiol (SH) groups. The dose-dependent changes were confirmed for SOD1 activity and DNA damage. The most sensitive parameters were SOD1 activity and DNA damage with the lowest BMDLs 0.1 μg F−/kg b. w. Since human and animal populations are daily and frequently unconsciously exposed to F−, this dose-response study is valuable for further research regarding the F− health risk assessment.
T2  - Environmental Pollution
T1  - Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage
VL  - 314
SP  - 120321
DO  - 10.1016/j.envpol.2022.120321
ER  - 

@article{
author = "Radovanović, Jelena and Antonijević, Biljana and Ćurčić, Marijana and Baralić, Katarina and Kolarević, Stoimir and Bulat, Zorica and Đukić-Ćosić, Danijela and Buha-Đorđević, Aleksandra and Vuković-Gačić, Branka and Javorac, Dragana and Antonijević Miljaković, Evica and Carević, Momir and Mandinić, Zoran",
year = "2022",
abstract = "Excessive fluoride (F−) levels in the environment could induce different pathological changes, including comorbidities in reproductive functions. Hence, the aim of the present in vivo study was to explore F− subacute toxicity mechanisms via Benchmark dose (BMD) methodology on rat's testicles. The experiment was conducted on thirty male Wistar rats for 28 days, divided into six groups (n = 5): 1) Control (tap water); 2) 10 mg/L F−; 3) 25 mg/L F−; 4) 50 mg/L F−; 5) 100 mg/L F−; 6) 150 mg/L F−. Testicles were dissected out and processed for the determination of F− tissue concentrations, redox status parameters, essential elements level, and DNA damage. PROASTweb 70.1 software was used for determination of external and internal dose-response relationship. The results confirmed a significant increase in superoxide anion (O2.-), total oxidative status (TOS), copper (Cu), zinc (Zn), iron (Fe), DNA damage levels, and decrease in superoxide dismutase activity (SOD1) and total thiol (SH) groups. The dose-dependent changes were confirmed for SOD1 activity and DNA damage. The most sensitive parameters were SOD1 activity and DNA damage with the lowest BMDLs 0.1 μg F−/kg b. w. Since human and animal populations are daily and frequently unconsciously exposed to F−, this dose-response study is valuable for further research regarding the F− health risk assessment.",
journal = "Environmental Pollution",
title = "Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage",
volume = "314",
pages = "120321",
doi = "10.1016/j.envpol.2022.120321"
}

Radovanović, J., Antonijević, B., Ćurčić, M., Baralić, K., Kolarević, S., Bulat, Z., Đukić-Ćosić, D., Buha-Đorđević, A., Vuković-Gačić, B., Javorac, D., Antonijević Miljaković, E., Carević, M.,& Mandinić, Z.. (2022). Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage. in Environmental Pollution, 314, 120321.
https://doi.org/10.1016/j.envpol.2022.120321

Radovanović J, Antonijević B, Ćurčić M, Baralić K, Kolarević S, Bulat Z, Đukić-Ćosić D, Buha-Đorđević A, Vuković-Gačić B, Javorac D, Antonijević Miljaković E, Carević M, Mandinić Z. Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage. in Environmental Pollution. 2022;314:120321.
doi:10.1016/j.envpol.2022.120321 .

Radovanović, Jelena, Antonijević, Biljana, Ćurčić, Marijana, Baralić, Katarina, Kolarević, Stoimir, Bulat, Zorica, Đukić-Ćosić, Danijela, Buha-Đorđević, Aleksandra, Vuković-Gačić, Branka, Javorac, Dragana, Antonijević Miljaković, Evica, Carević, Momir, Mandinić, Zoran, "Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage" in Environmental Pollution, 314 (2022):120321,
https://doi.org/10.1016/j.envpol.2022.120321 . .

TY  - JOUR
AU  - Radovanović, Jelena
AU  - Antonijević, Biljana
AU  - Baralić, Katarina
AU  - Ćurčić, Marijana
AU  - Đukić-Ćosić, Danijela
AU  - Bulat, Zorica
AU  - Javorac, Dragana
AU  - Buha-Đorđević, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Sudar-Milovanović, Emina
AU  - Antonijević-Miljaković, Evica
AU  - Beloica, Miloš
AU  - Mandinić, Zoran
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10464
AB  - This study aimed to investigate the effect of 150 mg/L sodium fluoride (NaF) on redox status parameters and essential metals [copper (Cu), iron (Fe), and zinc (Zn)] in the blood, liver, kidney, brain, and spleen of Wistar rats and to determine the protective potential of selenium (Se) against fluoride (F-) toxicity. Male Wistar rats were randomly distributed in groups of five (n=5) receiving tap water (control) or water with NaF 150 mg/L, NaF 150 mg/L + Se 1.5 mg/L, and Se 1.5 mg/L solutions ad libitum for 28 days. Fluorides caused an imbalance in the redox and biometal (Cu, Fe, and Zn) status, leading to high superoxide anion (O2 .-) and malondialdehyde (MDA) levels in the blood and brain and a drop in superoxide dismutase (SOD1) activity in the liver and its increase in the brain and kidneys. Se given with NaF improved MDA, SOD1, and O2 .- in the blood, brain, and kidneys, while alone it decreased SH group levels in the liver and kidney. Biometals both reduced and increased F- toxicity. Further research is needed before Se should be considered as a promising strategy for mitigating F- toxicity. © 2022 Jelena Radovanović, Biljana Antonijević, Katarina Baralić, Marijana Ćurčić, Danijela Đukić-Ćosić, Zorica Bulat, Dragana Javorac, Aleksandra Buha Đorđević, Jelena Kotur-Stevuljević, Emina Sudar-Milovanović, Evica Antonijević Miljaković, Miloš Beloica, and Zoran Mandinić, published by Sciendo.  Cilj ovog istraživanja bio je utvrditi djelovanje 150 mg/L natrijevog fluorida (NaF) na redoks-status i koncentracije esencijalnih elemenata [bakar (Cu), željezo (Fe) i cink (Zn)] u krvi, jetri, bubrezima, mozgu i slezeni Wistar štakora te mogući zaštitni učinak selena (Se) od toksičnosti prouzročene fluoridom (F-). Mužjaci Wistar štakora nasumično su razvrstani u četiri skupine (n=5), nakon čega su 28 dana konzumirali običnu vodu ili vodu s otopinom NaF 150 mg/L, NaF 150 mg/L + Se 1,5 mg/L ili Se 1,5 mg/L. Izloženost fluoridu dovela je do poremećaja redoks-parametara i koncentracija istraživanih biometala. Utvrđene su povišene razine superoksid aniona (O2.-) i malondialdehida (MDA) u krvi i mozgu, smanjena aktivnost superoksid dismutaze (SOD1) u jetri te njezin porast u mozgu i bubrezima. Nadomjesni Se u kombinaciji s NaF pozitivno je utjecao na razine MDA, SOD1 i O2.- u krvi, mozgu i bubrezima, a sâm Se smanjio je razine SH skupina u jetri i bubrezima. Izloženost fluoridu uzrokovala je sniženje, ali i porast koncentracija biometala. Nužna su dodatna istraživanja kako bi se ispitali antioksidacijski učinci Se na toksičnost izazvanu F-.
T2  - Arhiv za higijenu rada i toksikologiju
T1  - Redox and biometal status in Wistar rats after subacute exposure to fluoride and selenium counter-effects
VL  - 73
IS  - 3
SP  - 207
EP  - 222
DO  - 10.2478/aiht-2022-73-3650
ER  - 

@article{
author = "Radovanović, Jelena and Antonijević, Biljana and Baralić, Katarina and Ćurčić, Marijana and Đukić-Ćosić, Danijela and Bulat, Zorica and Javorac, Dragana and Buha-Đorđević, Aleksandra and Kotur-Stevuljević, Jelena and Sudar-Milovanović, Emina and Antonijević-Miljaković, Evica and Beloica, Miloš and Mandinić, Zoran",
year = "2022",
abstract = "This study aimed to investigate the effect of 150 mg/L sodium fluoride (NaF) on redox status parameters and essential metals [copper (Cu), iron (Fe), and zinc (Zn)] in the blood, liver, kidney, brain, and spleen of Wistar rats and to determine the protective potential of selenium (Se) against fluoride (F-) toxicity. Male Wistar rats were randomly distributed in groups of five (n=5) receiving tap water (control) or water with NaF 150 mg/L, NaF 150 mg/L + Se 1.5 mg/L, and Se 1.5 mg/L solutions ad libitum for 28 days. Fluorides caused an imbalance in the redox and biometal (Cu, Fe, and Zn) status, leading to high superoxide anion (O2 .-) and malondialdehyde (MDA) levels in the blood and brain and a drop in superoxide dismutase (SOD1) activity in the liver and its increase in the brain and kidneys. Se given with NaF improved MDA, SOD1, and O2 .- in the blood, brain, and kidneys, while alone it decreased SH group levels in the liver and kidney. Biometals both reduced and increased F- toxicity. Further research is needed before Se should be considered as a promising strategy for mitigating F- toxicity. © 2022 Jelena Radovanović, Biljana Antonijević, Katarina Baralić, Marijana Ćurčić, Danijela Đukić-Ćosić, Zorica Bulat, Dragana Javorac, Aleksandra Buha Đorđević, Jelena Kotur-Stevuljević, Emina Sudar-Milovanović, Evica Antonijević Miljaković, Miloš Beloica, and Zoran Mandinić, published by Sciendo.  Cilj ovog istraživanja bio je utvrditi djelovanje 150 mg/L natrijevog fluorida (NaF) na redoks-status i koncentracije esencijalnih elemenata [bakar (Cu), željezo (Fe) i cink (Zn)] u krvi, jetri, bubrezima, mozgu i slezeni Wistar štakora te mogući zaštitni učinak selena (Se) od toksičnosti prouzročene fluoridom (F-). Mužjaci Wistar štakora nasumično su razvrstani u četiri skupine (n=5), nakon čega su 28 dana konzumirali običnu vodu ili vodu s otopinom NaF 150 mg/L, NaF 150 mg/L + Se 1,5 mg/L ili Se 1,5 mg/L. Izloženost fluoridu dovela je do poremećaja redoks-parametara i koncentracija istraživanih biometala. Utvrđene su povišene razine superoksid aniona (O2.-) i malondialdehida (MDA) u krvi i mozgu, smanjena aktivnost superoksid dismutaze (SOD1) u jetri te njezin porast u mozgu i bubrezima. Nadomjesni Se u kombinaciji s NaF pozitivno je utjecao na razine MDA, SOD1 i O2.- u krvi, mozgu i bubrezima, a sâm Se smanjio je razine SH skupina u jetri i bubrezima. Izloženost fluoridu uzrokovala je sniženje, ali i porast koncentracija biometala. Nužna su dodatna istraživanja kako bi se ispitali antioksidacijski učinci Se na toksičnost izazvanu F-.",
journal = "Arhiv za higijenu rada i toksikologiju",
title = "Redox and biometal status in Wistar rats after subacute exposure to fluoride and selenium counter-effects",
volume = "73",
number = "3",
pages = "207-222",
doi = "10.2478/aiht-2022-73-3650"
}

Radovanović, J., Antonijević, B., Baralić, K., Ćurčić, M., Đukić-Ćosić, D., Bulat, Z., Javorac, D., Buha-Đorđević, A., Kotur-Stevuljević, J., Sudar-Milovanović, E., Antonijević-Miljaković, E., Beloica, M.,& Mandinić, Z.. (2022). Redox and biometal status in Wistar rats after subacute exposure to fluoride and selenium counter-effects. in Arhiv za higijenu rada i toksikologiju, 73(3), 207-222.
https://doi.org/10.2478/aiht-2022-73-3650

Radovanović J, Antonijević B, Baralić K, Ćurčić M, Đukić-Ćosić D, Bulat Z, Javorac D, Buha-Đorđević A, Kotur-Stevuljević J, Sudar-Milovanović E, Antonijević-Miljaković E, Beloica M, Mandinić Z. Redox and biometal status in Wistar rats after subacute exposure to fluoride and selenium counter-effects. in Arhiv za higijenu rada i toksikologiju. 2022;73(3):207-222.
doi:10.2478/aiht-2022-73-3650 .

Radovanović, Jelena, Antonijević, Biljana, Baralić, Katarina, Ćurčić, Marijana, Đukić-Ćosić, Danijela, Bulat, Zorica, Javorac, Dragana, Buha-Đorđević, Aleksandra, Kotur-Stevuljević, Jelena, Sudar-Milovanović, Emina, Antonijević-Miljaković, Evica, Beloica, Miloš, Mandinić, Zoran, "Redox and biometal status in Wistar rats after subacute exposure to fluoride and selenium counter-effects" in Arhiv za higijenu rada i toksikologiju, 73, no. 3 (2022):207-222,
https://doi.org/10.2478/aiht-2022-73-3650 . .

TY  - JOUR
AU  - Baralić, Katarina
AU  - Živančević, Katarina
AU  - Jorgovanović, Dragica
AU  - Bojanin, Dragana
AU  - Radovanović, Jelena
AU  - Gojković, Tamara
AU  - Buha-Đorđević, Aleksandra
AU  - Ćurčić, Marijana
AU  - Mandinić, Zoran
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
AU  - Đukić-Ćosić, Danijela
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9836
AB  - Linkage between bis(2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP), and bisphenol A (BPA) coexposure and type 2 diabetes mellitus (T2DM), as well as ability of multi-strained probiotic to reduce DEHP, DBP and BPA mixture-induced oxidative damage in rat pancreas were investigated. The Comparative Toxicogenomics Database, Cytoscape software and ToppGene Suite were used for data-mining. Animals were sorted into seven groups (n = 6): (1) Control group: corn oil, (2) P: probiotic: Saccharomyces boulardii + Lactobacillus rhamnosus + Lactobacillus plantarum LP 6595 + Lactobacillus plantarum HEAL9; (3) DEHP: 50 mg/kg b.w./day, (4) DBP: 50 mg/kg b.w./day, (5) BPA: 25 mg/kg b.w./day, and (6) MIX: 50 mg/kg b.w./day DEHP + 50 mg/kg b.w/ day DBP + 25 mg/kg b.w./day BPA; (7) MIX + P. Rats were sacrificed after 28 days of oral exposure. In silico investigation highlighted 44 DEHP, DBP and BPA mutual genes linked to the T2DM, while apoptosis and oxidative stress were highlighted as the main mechanisms of DEHP, DBP and BPA mixture-linked T2DM. In vivo experiment confirmed the presence of significant changes in redox status parameters (TOS, SOD and SH groups) only in the MIX group, indicating possible additive effects, while probiotic ameliorated mixture-induced redox status changes in rat pancreatic tissue.
T2  - Food and Chemical Toxicology
T1  - Probiotic reduced the impact of phthalates and bisphenol A mixture on type 2 diabetes mellitus development: Merging bioinformatics with in vivo analysis
VL  - 154
DO  - 10.1016/j.fct.2021.112325
ER  - 

@article{
author = "Baralić, Katarina and Živančević, Katarina and Jorgovanović, Dragica and Bojanin, Dragana and Radovanović, Jelena and Gojković, Tamara and Buha-Đorđević, Aleksandra and Ćurčić, Marijana and Mandinić, Zoran and Bulat, Zorica and Antonijević, Biljana and Đukić-Ćosić, Danijela",
year = "2021",
abstract = "Linkage between bis(2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP), and bisphenol A (BPA) coexposure and type 2 diabetes mellitus (T2DM), as well as ability of multi-strained probiotic to reduce DEHP, DBP and BPA mixture-induced oxidative damage in rat pancreas were investigated. The Comparative Toxicogenomics Database, Cytoscape software and ToppGene Suite were used for data-mining. Animals were sorted into seven groups (n = 6): (1) Control group: corn oil, (2) P: probiotic: Saccharomyces boulardii + Lactobacillus rhamnosus + Lactobacillus plantarum LP 6595 + Lactobacillus plantarum HEAL9; (3) DEHP: 50 mg/kg b.w./day, (4) DBP: 50 mg/kg b.w./day, (5) BPA: 25 mg/kg b.w./day, and (6) MIX: 50 mg/kg b.w./day DEHP + 50 mg/kg b.w/ day DBP + 25 mg/kg b.w./day BPA; (7) MIX + P. Rats were sacrificed after 28 days of oral exposure. In silico investigation highlighted 44 DEHP, DBP and BPA mutual genes linked to the T2DM, while apoptosis and oxidative stress were highlighted as the main mechanisms of DEHP, DBP and BPA mixture-linked T2DM. In vivo experiment confirmed the presence of significant changes in redox status parameters (TOS, SOD and SH groups) only in the MIX group, indicating possible additive effects, while probiotic ameliorated mixture-induced redox status changes in rat pancreatic tissue.",
journal = "Food and Chemical Toxicology",
title = "Probiotic reduced the impact of phthalates and bisphenol A mixture on type 2 diabetes mellitus development: Merging bioinformatics with in vivo analysis",
volume = "154",
doi = "10.1016/j.fct.2021.112325"
}

Baralić, K., Živančević, K., Jorgovanović, D., Bojanin, D., Radovanović, J., Gojković, T., Buha-Đorđević, A., Ćurčić, M., Mandinić, Z., Bulat, Z., Antonijević, B.,& Đukić-Ćosić, D.. (2021). Probiotic reduced the impact of phthalates and bisphenol A mixture on type 2 diabetes mellitus development: Merging bioinformatics with in vivo analysis. in Food and Chemical Toxicology, 154.
https://doi.org/10.1016/j.fct.2021.112325

Baralić K, Živančević K, Jorgovanović D, Bojanin D, Radovanović J, Gojković T, Buha-Đorđević A, Ćurčić M, Mandinić Z, Bulat Z, Antonijević B, Đukić-Ćosić D. Probiotic reduced the impact of phthalates and bisphenol A mixture on type 2 diabetes mellitus development: Merging bioinformatics with in vivo analysis. in Food and Chemical Toxicology. 2021;154.
doi:10.1016/j.fct.2021.112325 .

Baralić, Katarina, Živančević, Katarina, Jorgovanović, Dragica, Bojanin, Dragana, Radovanović, Jelena, Gojković, Tamara, Buha-Đorđević, Aleksandra, Ćurčić, Marijana, Mandinić, Zoran, Bulat, Zorica, Antonijević, Biljana, Đukić-Ćosić, Danijela, "Probiotic reduced the impact of phthalates and bisphenol A mixture on type 2 diabetes mellitus development: Merging bioinformatics with in vivo analysis" in Food and Chemical Toxicology, 154 (2021),
https://doi.org/10.1016/j.fct.2021.112325 . .