TY  - JOUR
AU  - Krstić, Danijela Z.
AU  - Tomić, Nenad
AU  - Radosavljević, Branimir
AU  - Avramović, Nataša
AU  - Dragutinović, Vesna
AU  - Radojević-Škodrić, Sanja
AU  - Čolović, Mirjana B.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1200
AB  - Kidney damage can be induced by ischemia, autoimmune diseases, hypertension, allograft rejection, metabolic or genetic disorders, infections or toxins. The influence of these factors could result in acute kidney injury (AKI) defined as an unexpected decrease in urine output or renal function, or encourage the development of chronic kidney disease (CKD). Biomarkers of renal function, measured in urine and serum, are in increasing use in order to estimate the severity and nature of kidney injury, and consequently apply appropriate therapy and improve patient management. The determined values of biomarkers can suggest the potential risk of kidney disease and the type of renal injury, predict the disease progression, as well as be helpful for assessing the response to an applied therapy. Although novel biomarkers are in practical use, serum creatinine, the indicator of glomerular filtration rate is still the most frequently used biomarker of renal function despite its known limitations. In recent decades, numerous studies resulted in discovering urinary and serum proteins that can serve as biomarkers for early and accurate detection of AKI and its development, as well as the identification of CKD. This review gives an overview of the most important renal biomarkers investigated in kidney diseases, classified in following types: functional biomarkers, up-regulated proteins, enzymes, and cycle arrest biomarkers. It describes their properties, physiological roles, and discusses the utility of these molecules in different clinical settings.
T2  - Current Medicinal Chemistry
T1  - Biochemical Markers of Renal Function
VL  - 23
IS  - 19
SP  - 2018
EP  - 2040
DO  - 10.2174/0929867323666160115130241
ER  - 

@article{
author = "Krstić, Danijela Z. and Tomić, Nenad and Radosavljević, Branimir and Avramović, Nataša and Dragutinović, Vesna and Radojević-Škodrić, Sanja and Čolović, Mirjana B.",
year = "2016",
abstract = "Kidney damage can be induced by ischemia, autoimmune diseases, hypertension, allograft rejection, metabolic or genetic disorders, infections or toxins. The influence of these factors could result in acute kidney injury (AKI) defined as an unexpected decrease in urine output or renal function, or encourage the development of chronic kidney disease (CKD). Biomarkers of renal function, measured in urine and serum, are in increasing use in order to estimate the severity and nature of kidney injury, and consequently apply appropriate therapy and improve patient management. The determined values of biomarkers can suggest the potential risk of kidney disease and the type of renal injury, predict the disease progression, as well as be helpful for assessing the response to an applied therapy. Although novel biomarkers are in practical use, serum creatinine, the indicator of glomerular filtration rate is still the most frequently used biomarker of renal function despite its known limitations. In recent decades, numerous studies resulted in discovering urinary and serum proteins that can serve as biomarkers for early and accurate detection of AKI and its development, as well as the identification of CKD. This review gives an overview of the most important renal biomarkers investigated in kidney diseases, classified in following types: functional biomarkers, up-regulated proteins, enzymes, and cycle arrest biomarkers. It describes their properties, physiological roles, and discusses the utility of these molecules in different clinical settings.",
journal = "Current Medicinal Chemistry",
title = "Biochemical Markers of Renal Function",
volume = "23",
number = "19",
pages = "2018-2040",
doi = "10.2174/0929867323666160115130241"
}

Krstić, D. Z., Tomić, N., Radosavljević, B., Avramović, N., Dragutinović, V., Radojević-Škodrić, S.,& Čolović, M. B.. (2016). Biochemical Markers of Renal Function. in Current Medicinal Chemistry, 23(19), 2018-2040.
https://doi.org/10.2174/0929867323666160115130241

Krstić DZ, Tomić N, Radosavljević B, Avramović N, Dragutinović V, Radojević-Škodrić S, Čolović MB. Biochemical Markers of Renal Function. in Current Medicinal Chemistry. 2016;23(19):2018-2040.
doi:10.2174/0929867323666160115130241 .

Krstić, Danijela Z., Tomić, Nenad, Radosavljević, Branimir, Avramović, Nataša, Dragutinović, Vesna, Radojević-Škodrić, Sanja, Čolović, Mirjana B., "Biochemical Markers of Renal Function" in Current Medicinal Chemistry, 23, no. 19 (2016):2018-2040,
https://doi.org/10.2174/0929867323666160115130241 . .

TY  - CONF
AU  - Čolović, Mirjana
AU  - Vasić, Vesna M.
AU  - Avramović, Nataša
AU  - Krstić, Danijela
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13013
AB  - The aim of this study was to investigate neurotoxic potential and oxidative stress responses of diazinon and its metabolites, diazoxon and 2-isopropyl-6-methyl-4-pyrimidinol using synaptosomes as a model system. Synaptosomes were isolated from the brain of Wistar albino rats and incubated at 37oC for 1 hour in the presence of selected concentrations of the investigated compounds. Acetylcholinesterase, Na+/K+-ATPase and antioxidant enzymes activities were determined by standard spectrophotometric methods. Diazinon induced concentration-dependent acetylcholinesterase and Na+/K+-ATPase inhibition, while the activity of catalase, superoxide dismutase and glutathione peroxidase was not significantly affected. Increasing concentrations of diazoxon, oxo analog of diazinon, caused almost complete acetylcholinesterase and Na+/K+-ATPase inhibition, and activated antioxidant enzymes: catalase (up to 25%), superoxide dismutase (up to 55%) and glutathione peroxidase (up to 30%). Unlike diazoxon, diazinon hydrolysis product, 2-isopropyl-6-methyl-4-pyrimidinol did not remarkably change the activities of the investigated enzymes, except superoxide dismutase that was stimulated up to 25%. The obtained results suggest that neurotoxic and prooxidative potential of diazinon, thioorganophoshate used as a commercial insecticide preparation, significantly reinforces mostly due to its transformation to diazoxon in the metabolic pathways.
PB  - Niš : RAD Association
C3  - RAD 2015 : International Conference on Radiation and Applications in Various Fields of Research : Book of abstracts
T1  - The influence of diazinon and its metabolites on acetylcholinesterase, NA+/K+-ATPase and antioxidant enzymes in rat brain synaptosomes
SP  - 15
EP  - 15
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13013
ER  - 

@conference{
author = "Čolović, Mirjana and Vasić, Vesna M. and Avramović, Nataša and Krstić, Danijela",
year = "2015",
abstract = "The aim of this study was to investigate neurotoxic potential and oxidative stress responses of diazinon and its metabolites, diazoxon and 2-isopropyl-6-methyl-4-pyrimidinol using synaptosomes as a model system. Synaptosomes were isolated from the brain of Wistar albino rats and incubated at 37oC for 1 hour in the presence of selected concentrations of the investigated compounds. Acetylcholinesterase, Na+/K+-ATPase and antioxidant enzymes activities were determined by standard spectrophotometric methods. Diazinon induced concentration-dependent acetylcholinesterase and Na+/K+-ATPase inhibition, while the activity of catalase, superoxide dismutase and glutathione peroxidase was not significantly affected. Increasing concentrations of diazoxon, oxo analog of diazinon, caused almost complete acetylcholinesterase and Na+/K+-ATPase inhibition, and activated antioxidant enzymes: catalase (up to 25%), superoxide dismutase (up to 55%) and glutathione peroxidase (up to 30%). Unlike diazoxon, diazinon hydrolysis product, 2-isopropyl-6-methyl-4-pyrimidinol did not remarkably change the activities of the investigated enzymes, except superoxide dismutase that was stimulated up to 25%. The obtained results suggest that neurotoxic and prooxidative potential of diazinon, thioorganophoshate used as a commercial insecticide preparation, significantly reinforces mostly due to its transformation to diazoxon in the metabolic pathways.",
publisher = "Niš : RAD Association",
journal = "RAD 2015 : International Conference on Radiation and Applications in Various Fields of Research : Book of abstracts",
title = "The influence of diazinon and its metabolites on acetylcholinesterase, NA+/K+-ATPase and antioxidant enzymes in rat brain synaptosomes",
pages = "15-15",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13013"
}

Čolović, M., Vasić, V. M., Avramović, N.,& Krstić, D.. (2015). The influence of diazinon and its metabolites on acetylcholinesterase, NA+/K+-ATPase and antioxidant enzymes in rat brain synaptosomes. in RAD 2015 : International Conference on Radiation and Applications in Various Fields of Research : Book of abstracts
Niš : RAD Association., 15-15.
https://hdl.handle.net/21.15107/rcub_vinar_13013

Čolović M, Vasić VM, Avramović N, Krstić D. The influence of diazinon and its metabolites on acetylcholinesterase, NA+/K+-ATPase and antioxidant enzymes in rat brain synaptosomes. in RAD 2015 : International Conference on Radiation and Applications in Various Fields of Research : Book of abstracts. 2015;:15-15.
https://hdl.handle.net/21.15107/rcub_vinar_13013 .

Čolović, Mirjana, Vasić, Vesna M., Avramović, Nataša, Krstić, Danijela, "The influence of diazinon and its metabolites on acetylcholinesterase, NA+/K+-ATPase and antioxidant enzymes in rat brain synaptosomes" in RAD 2015 : International Conference on Radiation and Applications in Various Fields of Research : Book of abstracts (2015):15-15,
https://hdl.handle.net/21.15107/rcub_vinar_13013 .

TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Vasić, Vesna M.
AU  - Avramović, Nataša
AU  - Gajic, Milan M.
AU  - Đurić, Dragan M.
AU  - Krstić, Danijela Z.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/409
AB  - Although primary toxic action of organophosphorous insecticides is associated with acetylcholinesterase inhibition, later studies suggest that oxidative stress may be responsible for induced organophosphates toxicity. These studies mostly include thio forms, while the effects of their metabolites/ degradation products have been less investigated. Therefore, this paper studies the toxic effects of diazinon degradation products, diazoxon and 2-isopropyl-6-methyl-4-pyrimidinol, and compares them with the toxic potential of the parent compound. The toxicity induced by various concentrations of the investigated compounds was in vitro evaluated by the activities of acetylcholinesterase, ATPases, antioxidant defense enzymes and lactate dehydrogenase, and malondialdehyde level in rat brain synaptosomes. Diazinon inhibited acetylcholinesterase and Na+/K+-ATPase in dose-dependent manner, while the inhibition of ecto-ATPase activity was less than 15% at all investigated concentrations. It did not demonstrate noteworthy prooxidative properties causing increase (up to 10%) in antioxidant enzymes activity and malondialdehyde level, as a marker of lipid peroxidation. Diazinon oxidation product, diazoxon was found as the most toxic investigated compound. Beside the expected strong inhibitory effect on acetylcholinesterase, it induced dose-dependent and almost complete inhibition of Na+/K+-ATPase and ecto-ATPase at the highest investigated concentration (0.1 mM). Increasing diazoxon concentrations activated catalase (up to 30%), superoxide dismutase (up to 50%), glutathione peroxidase (up to 30%), and significantly increased malondialdehyde level (up to 50%). The investigated hydrolysis product of diazinon, 2-isopropyl-6-methyl-4-pyrimidinol did not remarkably alter the activities of acetylcholinesterase, Na+/K+-ATPase, catalase, glutathione peroxidase and lipid peroxidation level (up to about 10%). Although this diazinon metabolite has been known as non toxic, it induced superoxide dismutase stimulation up to 30%. Finally, even high concentrations of both diazinon and its metabolites did noticeably affect lactate dehydrogenase activity as a marker of synaptosomal integrity. The changes in investigated biochemical parameters in rat brain synaptosomes could serve as indicators of toxicity due to the exposure to thio organophosphates and/or their break-down products. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
T2  - Toxicology Letters
T1  - In vitro evaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes
VL  - 233
IS  - 1
SP  - 29
EP  - 37
DO  - 10.1016/j.toxlet.2015.01.003
ER  - 

@article{
author = "Čolović, Mirjana B. and Vasić, Vesna M. and Avramović, Nataša and Gajic, Milan M. and Đurić, Dragan M. and Krstić, Danijela Z.",
year = "2015",
abstract = "Although primary toxic action of organophosphorous insecticides is associated with acetylcholinesterase inhibition, later studies suggest that oxidative stress may be responsible for induced organophosphates toxicity. These studies mostly include thio forms, while the effects of their metabolites/ degradation products have been less investigated. Therefore, this paper studies the toxic effects of diazinon degradation products, diazoxon and 2-isopropyl-6-methyl-4-pyrimidinol, and compares them with the toxic potential of the parent compound. The toxicity induced by various concentrations of the investigated compounds was in vitro evaluated by the activities of acetylcholinesterase, ATPases, antioxidant defense enzymes and lactate dehydrogenase, and malondialdehyde level in rat brain synaptosomes. Diazinon inhibited acetylcholinesterase and Na+/K+-ATPase in dose-dependent manner, while the inhibition of ecto-ATPase activity was less than 15% at all investigated concentrations. It did not demonstrate noteworthy prooxidative properties causing increase (up to 10%) in antioxidant enzymes activity and malondialdehyde level, as a marker of lipid peroxidation. Diazinon oxidation product, diazoxon was found as the most toxic investigated compound. Beside the expected strong inhibitory effect on acetylcholinesterase, it induced dose-dependent and almost complete inhibition of Na+/K+-ATPase and ecto-ATPase at the highest investigated concentration (0.1 mM). Increasing diazoxon concentrations activated catalase (up to 30%), superoxide dismutase (up to 50%), glutathione peroxidase (up to 30%), and significantly increased malondialdehyde level (up to 50%). The investigated hydrolysis product of diazinon, 2-isopropyl-6-methyl-4-pyrimidinol did not remarkably alter the activities of acetylcholinesterase, Na+/K+-ATPase, catalase, glutathione peroxidase and lipid peroxidation level (up to about 10%). Although this diazinon metabolite has been known as non toxic, it induced superoxide dismutase stimulation up to 30%. Finally, even high concentrations of both diazinon and its metabolites did noticeably affect lactate dehydrogenase activity as a marker of synaptosomal integrity. The changes in investigated biochemical parameters in rat brain synaptosomes could serve as indicators of toxicity due to the exposure to thio organophosphates and/or their break-down products. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
journal = "Toxicology Letters",
title = "In vitro evaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes",
volume = "233",
number = "1",
pages = "29-37",
doi = "10.1016/j.toxlet.2015.01.003"
}

Čolović, M. B., Vasić, V. M., Avramović, N., Gajic, M. M., Đurić, D. M.,& Krstić, D. Z.. (2015). In vitro evaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes. in Toxicology Letters, 233(1), 29-37.
https://doi.org/10.1016/j.toxlet.2015.01.003

Čolović MB, Vasić VM, Avramović N, Gajic MM, Đurić DM, Krstić DZ. In vitro evaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes. in Toxicology Letters. 2015;233(1):29-37.
doi:10.1016/j.toxlet.2015.01.003 .

Čolović, Mirjana B., Vasić, Vesna M., Avramović, Nataša, Gajic, Milan M., Đurić, Dragan M., Krstić, Danijela Z., "In vitro evaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes" in Toxicology Letters, 233, no. 1 (2015):29-37,
https://doi.org/10.1016/j.toxlet.2015.01.003 . .

TY  - CONF
AU  - Čolović, Mirjana B.
AU  - Vasić, Vesna M.
AU  - Avramović, Nataša
AU  - Đurić, Dragan M.
AU  - Krstić, Danijela Z.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9189
AB  - Toxic effects of diazinon and its degradation products, diazoxon and 2- isopropyl-6-methyl-4-pyrimidinol (IMP), were investigated in vitro by determining the inhibition of acetylcholinesterase (AChE), Na+ /K+ -ATPase and ecto-ATPase activity in rat brain synaptosomes after 1 hour exposure toward varying concentrations. Dose-dependent AChE and Na+ /K+ -ATPase inhibition was obtained in the presence of diazinon, while diazinon concentrations below 0.1 mM did not noticeably affect ecto-ATPase activity. Diazinon oxidation product, diazoxon was found as the most toxic investigated compound. Diazoxon induced dose-dependent and almost complete inhibition of AChE, Na+ /K+ -ATPase and ecto-ATPase at the highest investigated concentration (0.1 mM), while hydrolysis product of diazinon, IMP did not remarkably influence their activities.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - In vitro evaluation of diazinon and its degradation products neurotoxicity potential in rat brain synaptosomes
VL  - F-05-P
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9189
ER  - 

@conference{
author = "Čolović, Mirjana B. and Vasić, Vesna M. and Avramović, Nataša and Đurić, Dragan M. and Krstić, Danijela Z.",
year = "2014",
abstract = "Toxic effects of diazinon and its degradation products, diazoxon and 2- isopropyl-6-methyl-4-pyrimidinol (IMP), were investigated in vitro by determining the inhibition of acetylcholinesterase (AChE), Na+ /K+ -ATPase and ecto-ATPase activity in rat brain synaptosomes after 1 hour exposure toward varying concentrations. Dose-dependent AChE and Na+ /K+ -ATPase inhibition was obtained in the presence of diazinon, while diazinon concentrations below 0.1 mM did not noticeably affect ecto-ATPase activity. Diazinon oxidation product, diazoxon was found as the most toxic investigated compound. Diazoxon induced dose-dependent and almost complete inhibition of AChE, Na+ /K+ -ATPase and ecto-ATPase at the highest investigated concentration (0.1 mM), while hydrolysis product of diazinon, IMP did not remarkably influence their activities.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "In vitro evaluation of diazinon and its degradation products neurotoxicity potential in rat brain synaptosomes",
volume = "F-05-P",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9189"
}

Čolović, M. B., Vasić, V. M., Avramović, N., Đurić, D. M.,& Krstić, D. Z.. (2014). In vitro evaluation of diazinon and its degradation products neurotoxicity potential in rat brain synaptosomes. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., F-05-P.
https://hdl.handle.net/21.15107/rcub_vinar_9189

Čolović MB, Vasić VM, Avramović N, Đurić DM, Krstić DZ. In vitro evaluation of diazinon and its degradation products neurotoxicity potential in rat brain synaptosomes. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2014;F-05-P.
https://hdl.handle.net/21.15107/rcub_vinar_9189 .

Čolović, Mirjana B., Vasić, Vesna M., Avramović, Nataša, Đurić, Dragan M., Krstić, Danijela Z., "In vitro evaluation of diazinon and its degradation products neurotoxicity potential in rat brain synaptosomes" in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry, F-05-P (2014),
https://hdl.handle.net/21.15107/rcub_vinar_9189 .

TY  - CONF
AU  - Čolović, Mirjana B.
AU  - Vasić, Vesna M.
AU  - Avramović, Nataša
AU  - Đurić, Dragan M.
AU  - Krstić, Danijela Z.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9195
AB  - In vitro evaluation of oxidative stress responses to various concentrations of diazinon and its degradation products, diazoxon and 2-isopropyl-6-methyl- 4-pyrimidinol (IMP) was investigated by determining antioxidant enzymes activity (catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx)) and lipid peroxidation level in rat brain synaptosomes. Diazinon showed negligible prooxidative properties causing increase in antioxidant enzymes activity and lipid peroxidation level up to 10%. Increasing concentrations of diazinon oxidation product, diazoxon activated CAT (up to 20%), SOD (up to 50%), GPx (up to 25%), and significantly increased the content of lipid peroxidation indicator (up to 50%). The investigated hydrolysis product of diazinon, IMP did not remarkably influence the activity of CAT, GPx and lipid peroxidation level (up to 10%), while it induced SOD stimulation up to 30%.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - The effects of diazinon and its degradation products on oxidative stress parameters in rat brain synaptosomes
VL  - F-24-P
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9195
ER  - 

@conference{
author = "Čolović, Mirjana B. and Vasić, Vesna M. and Avramović, Nataša and Đurić, Dragan M. and Krstić, Danijela Z.",
year = "2014",
abstract = "In vitro evaluation of oxidative stress responses to various concentrations of diazinon and its degradation products, diazoxon and 2-isopropyl-6-methyl- 4-pyrimidinol (IMP) was investigated by determining antioxidant enzymes activity (catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx)) and lipid peroxidation level in rat brain synaptosomes. Diazinon showed negligible prooxidative properties causing increase in antioxidant enzymes activity and lipid peroxidation level up to 10%. Increasing concentrations of diazinon oxidation product, diazoxon activated CAT (up to 20%), SOD (up to 50%), GPx (up to 25%), and significantly increased the content of lipid peroxidation indicator (up to 50%). The investigated hydrolysis product of diazinon, IMP did not remarkably influence the activity of CAT, GPx and lipid peroxidation level (up to 10%), while it induced SOD stimulation up to 30%.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "The effects of diazinon and its degradation products on oxidative stress parameters in rat brain synaptosomes",
volume = "F-24-P",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9195"
}

Čolović, M. B., Vasić, V. M., Avramović, N., Đurić, D. M.,& Krstić, D. Z.. (2014). The effects of diazinon and its degradation products on oxidative stress parameters in rat brain synaptosomes. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., F-24-P.
https://hdl.handle.net/21.15107/rcub_vinar_9195

Čolović MB, Vasić VM, Avramović N, Đurić DM, Krstić DZ. The effects of diazinon and its degradation products on oxidative stress parameters in rat brain synaptosomes. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2014;F-24-P.
https://hdl.handle.net/21.15107/rcub_vinar_9195 .

Čolović, Mirjana B., Vasić, Vesna M., Avramović, Nataša, Đurić, Dragan M., Krstić, Danijela Z., "The effects of diazinon and its degradation products on oxidative stress parameters in rat brain synaptosomes" in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry, F-24-P (2014),
https://hdl.handle.net/21.15107/rcub_vinar_9195 .

TY  - JOUR
AU  - Avramović, Nataša
AU  - Dragutinovic, V
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Trbovic, A.
AU  - De Luka, Silvio R.
AU  - Milovanović, I
AU  - Popovic, T.
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5239
AB  - Background: The omega 3 fatty acids play an important role in many physiological processes. Their effect is well documented in neurodegenerative diseases and inflammatory diseases. Also, aging as a biophysiological process could be influenced by eicosapentanoic acid (EPA) and docosahexanoic acid (DHA) components of fish oil. However there are not many studies showing the effect of PUFA (polyunsaturated FA) suplementation in eldery brain functions and the response to oxidative strees. The aim of this study was to investigate the effects of dietary omega-3 fatty acid supplementation on levels of lipid peroxidation and oxidant/antioxidant status of brain tissue in aged (24 months old) Wistar rats. Methods: Animals were divided in two groups. Control group (n=8) was fed with standard laboratory food and received water ad libitum. Treated group (n=8) was also fed with standard laboratory food, water ad libitum and received fish oil capsules (EPA+DHA) for 6 weeks. Daily dose was 30mg EPA and 45mg DHA (capsules: 200mg EPA and 300mg DHA; in-house method). At the end of treatment animals were sacrificed and brains were collected and frozen on -80 degrees C. The levels of lipid peroxidation (malondialdehyde - MDA), activity of catalase (CAT) and activity of superoxide dismutase (SOD) were examined in cerebral cortex. Catalase activity was determined by measuring the decrease in absorbance (H2O2 degradation) at 240 nm for 3 min and expressed as U/mg protein. Total SOD (superoxide dismutase) activity was performed at room temperature according to the method of Misra and Fridovich. The extent of lipid peroxidation (LPO) was estimated as the concentration of thiobarbituric acid reactive product malondialdehyde (MDA) by using the method of Aruoma et al. The incorporation of fatty acids in cellular membranes was confirmed by gas chromatography. Results: Our results showed that lipid peroxidation significantly decreased in treated animal group, where MDA concentration was 0.38 +/- 0.001 vs. 0.43 +/- 0.001 nM/ml (p LT 0.05) in control. However SOD activity increased significantly in treated animal group 1.57 +/- 0.24 vs. 4.12 +/- 0.15 U/gHb/L (p LT 0.01) in control. CAT activity decreased in treated group but not significantly. Conclusion: Incorporation of omega-3 fatty acids after their supplementation had beneficial effects on brain tissue. Omega-3 fatty acids increased activity of SOD and decreased lipid peroxidation. Changes in oxidative/antioxidative balance are a result of EPA and DHA effects on lipids and enzymes of antioxidative system. Hippokratia 2012; 16 (3): 214-245
T2  - Hippokratia
T1  - The effects of omega 3 fatty acid supplementation on brain tissue oxidative status in aged wistar rats
VL  - 16
IS  - 3
SP  - 241
EP  - 245
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5239
ER  - 

@article{
author = "Avramović, Nataša and Dragutinovic, V and Krstić, Danijela Z. and Čolović, Mirjana B. and Trbovic, A. and De Luka, Silvio R. and Milovanović, I and Popovic, T.",
year = "2012",
abstract = "Background: The omega 3 fatty acids play an important role in many physiological processes. Their effect is well documented in neurodegenerative diseases and inflammatory diseases. Also, aging as a biophysiological process could be influenced by eicosapentanoic acid (EPA) and docosahexanoic acid (DHA) components of fish oil. However there are not many studies showing the effect of PUFA (polyunsaturated FA) suplementation in eldery brain functions and the response to oxidative strees. The aim of this study was to investigate the effects of dietary omega-3 fatty acid supplementation on levels of lipid peroxidation and oxidant/antioxidant status of brain tissue in aged (24 months old) Wistar rats. Methods: Animals were divided in two groups. Control group (n=8) was fed with standard laboratory food and received water ad libitum. Treated group (n=8) was also fed with standard laboratory food, water ad libitum and received fish oil capsules (EPA+DHA) for 6 weeks. Daily dose was 30mg EPA and 45mg DHA (capsules: 200mg EPA and 300mg DHA; in-house method). At the end of treatment animals were sacrificed and brains were collected and frozen on -80 degrees C. The levels of lipid peroxidation (malondialdehyde - MDA), activity of catalase (CAT) and activity of superoxide dismutase (SOD) were examined in cerebral cortex. Catalase activity was determined by measuring the decrease in absorbance (H2O2 degradation) at 240 nm for 3 min and expressed as U/mg protein. Total SOD (superoxide dismutase) activity was performed at room temperature according to the method of Misra and Fridovich. The extent of lipid peroxidation (LPO) was estimated as the concentration of thiobarbituric acid reactive product malondialdehyde (MDA) by using the method of Aruoma et al. The incorporation of fatty acids in cellular membranes was confirmed by gas chromatography. Results: Our results showed that lipid peroxidation significantly decreased in treated animal group, where MDA concentration was 0.38 +/- 0.001 vs. 0.43 +/- 0.001 nM/ml (p LT 0.05) in control. However SOD activity increased significantly in treated animal group 1.57 +/- 0.24 vs. 4.12 +/- 0.15 U/gHb/L (p LT 0.01) in control. CAT activity decreased in treated group but not significantly. Conclusion: Incorporation of omega-3 fatty acids after their supplementation had beneficial effects on brain tissue. Omega-3 fatty acids increased activity of SOD and decreased lipid peroxidation. Changes in oxidative/antioxidative balance are a result of EPA and DHA effects on lipids and enzymes of antioxidative system. Hippokratia 2012; 16 (3): 214-245",
journal = "Hippokratia",
title = "The effects of omega 3 fatty acid supplementation on brain tissue oxidative status in aged wistar rats",
volume = "16",
number = "3",
pages = "241-245",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5239"
}

Avramović, N., Dragutinovic, V., Krstić, D. Z., Čolović, M. B., Trbovic, A., De Luka, S. R., Milovanović, I.,& Popovic, T.. (2012). The effects of omega 3 fatty acid supplementation on brain tissue oxidative status in aged wistar rats. in Hippokratia, 16(3), 241-245.
https://hdl.handle.net/21.15107/rcub_vinar_5239

Avramović N, Dragutinovic V, Krstić DZ, Čolović MB, Trbovic A, De Luka SR, Milovanović I, Popovic T. The effects of omega 3 fatty acid supplementation on brain tissue oxidative status in aged wistar rats. in Hippokratia. 2012;16(3):241-245.
https://hdl.handle.net/21.15107/rcub_vinar_5239 .

Avramović, Nataša, Dragutinovic, V, Krstić, Danijela Z., Čolović, Mirjana B., Trbovic, A., De Luka, Silvio R., Milovanović, I, Popovic, T., "The effects of omega 3 fatty acid supplementation on brain tissue oxidative status in aged wistar rats" in Hippokratia, 16, no. 3 (2012):241-245,
https://hdl.handle.net/21.15107/rcub_vinar_5239 .

TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Bajuk-Bogdanović, Danica V.
AU  - Avramović, Nataša
AU  - Holclajtner-Antunović, Ivanka D.
AU  - Bošnjaković-Pavlović, Nada
AU  - Vasić, Vesna M.
AU  - Krstić, Danijela Z.
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4558
AB  - C. The in vitro influence of Keggin structure polyoxotungstates, 12-tungstosilicic acid, H4SiW12O40 (WSiA) and 12-tungstophosphoric acid, H3PW12O40 (WPA), and monomer Na2WO4 x 2H(2)O on rat synaptic plasma membrane (SPM) Na+/K+-ATPase and E-NTPDase activity was studied, whereas the commercial porcine cerebral cortex Na+/K+-ATPase served as a reference. Dose-dependent Na+/K+-ATPase inhibition was obtained for all investigated compounds. Calculated IC50 (10 min) values, in mol/l, for SPM/commercial Na+/K+-ATPase, were: 3.4 x 10(-6)/4.3 x 10(-6), 2.9 x 10(-6)/3.1 x 10(-6) and 1.3 x 10(-3)/1.5 x 10(-3) for WSiA, WPA and Na2WO4 x 2H(2)O, respectively. In the case of E-NTPDase, increasing concentrations of WSiA and WPA induced its activity reduction, while Na2WO4 x 2H(2)O did not noticeably affect the enzyme activity at all investigated concentrations (up to 1 x 10(-3) mol/l). IC50 (10 min) values, obtained from the inhibition curves, were (in mol/l): 4.1 x 10(-6) for WSiA and 1.6 x 10(-6) for WPA. Monolacunary Keggin anion was found as the main active molecular species present under physiological conditions (in the enzyme assays, pH 7.4), for the both polyoxotungstates solutions (1 mmol/l), using Fourier transform infrared (FT-IR) and micro-Raman spectroscopy. Additionally, commercial porcine cerebral cortex Na+/K+-ATPase was exposed to the mixture of Na2WO4 x 2H(2)O and WSiA at different concentrations. Additive inhibition effect was achieved for lower concentrations of Na2WO4 x 2H(2)O/WSiA ( LT = 1 x 10(-3)/ 4 x 10(-6) mol/l), while antagonistic effect was obtained for all higher concentrations of the inhibitors. (C) 2011 Elsevier Ltd. All rights reserved.
T2  - Bioorganic and Medicinal Chemistry
T1  - Inhibition of rat synaptic membrane Na+/K+-ATPase and ecto-nucleoside triphosphate diphosphohydrolases by 12-tungstosilicic and 12-tungstophosphoric acid
VL  - 19
IS  - 23
SP  - 7063
EP  - 7069
DO  - 10.1016/j.bmc.2011.10.008
ER  - 

@article{
author = "Čolović, Mirjana B. and Bajuk-Bogdanović, Danica V. and Avramović, Nataša and Holclajtner-Antunović, Ivanka D. and Bošnjaković-Pavlović, Nada and Vasić, Vesna M. and Krstić, Danijela Z.",
year = "2011",
abstract = "C. The in vitro influence of Keggin structure polyoxotungstates, 12-tungstosilicic acid, H4SiW12O40 (WSiA) and 12-tungstophosphoric acid, H3PW12O40 (WPA), and monomer Na2WO4 x 2H(2)O on rat synaptic plasma membrane (SPM) Na+/K+-ATPase and E-NTPDase activity was studied, whereas the commercial porcine cerebral cortex Na+/K+-ATPase served as a reference. Dose-dependent Na+/K+-ATPase inhibition was obtained for all investigated compounds. Calculated IC50 (10 min) values, in mol/l, for SPM/commercial Na+/K+-ATPase, were: 3.4 x 10(-6)/4.3 x 10(-6), 2.9 x 10(-6)/3.1 x 10(-6) and 1.3 x 10(-3)/1.5 x 10(-3) for WSiA, WPA and Na2WO4 x 2H(2)O, respectively. In the case of E-NTPDase, increasing concentrations of WSiA and WPA induced its activity reduction, while Na2WO4 x 2H(2)O did not noticeably affect the enzyme activity at all investigated concentrations (up to 1 x 10(-3) mol/l). IC50 (10 min) values, obtained from the inhibition curves, were (in mol/l): 4.1 x 10(-6) for WSiA and 1.6 x 10(-6) for WPA. Monolacunary Keggin anion was found as the main active molecular species present under physiological conditions (in the enzyme assays, pH 7.4), for the both polyoxotungstates solutions (1 mmol/l), using Fourier transform infrared (FT-IR) and micro-Raman spectroscopy. Additionally, commercial porcine cerebral cortex Na+/K+-ATPase was exposed to the mixture of Na2WO4 x 2H(2)O and WSiA at different concentrations. Additive inhibition effect was achieved for lower concentrations of Na2WO4 x 2H(2)O/WSiA ( LT = 1 x 10(-3)/ 4 x 10(-6) mol/l), while antagonistic effect was obtained for all higher concentrations of the inhibitors. (C) 2011 Elsevier Ltd. All rights reserved.",
journal = "Bioorganic and Medicinal Chemistry",
title = "Inhibition of rat synaptic membrane Na+/K+-ATPase and ecto-nucleoside triphosphate diphosphohydrolases by 12-tungstosilicic and 12-tungstophosphoric acid",
volume = "19",
number = "23",
pages = "7063-7069",
doi = "10.1016/j.bmc.2011.10.008"
}

Čolović, M. B., Bajuk-Bogdanović, D. V., Avramović, N., Holclajtner-Antunović, I. D., Bošnjaković-Pavlović, N., Vasić, V. M.,& Krstić, D. Z.. (2011). Inhibition of rat synaptic membrane Na+/K+-ATPase and ecto-nucleoside triphosphate diphosphohydrolases by 12-tungstosilicic and 12-tungstophosphoric acid. in Bioorganic and Medicinal Chemistry, 19(23), 7063-7069.
https://doi.org/10.1016/j.bmc.2011.10.008

Čolović MB, Bajuk-Bogdanović DV, Avramović N, Holclajtner-Antunović ID, Bošnjaković-Pavlović N, Vasić VM, Krstić DZ. Inhibition of rat synaptic membrane Na+/K+-ATPase and ecto-nucleoside triphosphate diphosphohydrolases by 12-tungstosilicic and 12-tungstophosphoric acid. in Bioorganic and Medicinal Chemistry. 2011;19(23):7063-7069.
doi:10.1016/j.bmc.2011.10.008 .

Čolović, Mirjana B., Bajuk-Bogdanović, Danica V., Avramović, Nataša, Holclajtner-Antunović, Ivanka D., Bošnjaković-Pavlović, Nada, Vasić, Vesna M., Krstić, Danijela Z., "Inhibition of rat synaptic membrane Na+/K+-ATPase and ecto-nucleoside triphosphate diphosphohydrolases by 12-tungstosilicic and 12-tungstophosphoric acid" in Bioorganic and Medicinal Chemistry, 19, no. 23 (2011):7063-7069,
https://doi.org/10.1016/j.bmc.2011.10.008 . .