TY  - CONF
AU  - Isaković, Anđelka
AU  - Jeremić, Marija
AU  - Krstić, Danijela
AU  - Čolović, Mirjana
AU  - Kortz, Ulrich
AU  - Misirlić-Denčić, Sonja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12964
AB  - Background: Polyoxometalates are a class of anionic, polynuclear metal-oxo clusters reported as promising in vitro and in vivo antitumor agents for several decades. The aim of this study was to investigate the antineuroblastoma potential of the polyoxopalladate(II) nanocube Na8[Pd13As8O34(OH)6]・42H2O (Pd13). Material and methods: All experiments were performed on human neuroblastoma cell line, SH-SY5Y. The number of viable cells after the treatment with Pd13 was assessed using an acid phosphatase viability assay. The level of superoxide ion, mitochondrial membrane potential, pan-caspase activity, acidic intracellular vesicles content, and the cell cycle was determined by flow cytometry. Results: The obtained results suggest that Pd13 caused a significant decrease in cell viability with IC50 values of 7.7 µM (24 h) and 4.4 µM (48 h). Pd13 induced depolarization of mitochondrial membrane (2 h), followed by ~ 30% increase in the production of the superoxide ion (O2-) 4 h after treatment. An increase (~ 30%) in pancaspase activation and disturbance of neuroblastma cell cycle were observed after 24 h treatment. Namely, Pd13 caused an increase (14.4%) in the number of cells with fragmented nuclear DNA (SubG0), a decrease (%) of cells in the G1 phase, and an increase (%) in the S phase, all suggestive of cell cycle arrest. Finally, Pd13 increased the orange to green fluorescence ratio for ~ 45% 24 h after treatment, supporting intracellular acidification. Conclusion: The polyoxopalladate, Pd13 can be regarded as a promising antineuroblastoma agent which induces oxidative stress, and causes pan-caspase activation, DNA fragmentation and cell cycle arrest, which are all hallmarks of apoptotic neuroblastoma cell death.
PB  - Belgrade : Serbian Association for Cancer Research (SDIR)
C3  - SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts
T1  - Antineuroblastoma potential of polyoxopalladate(II)
SP  - 69
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12964
ER  - 

@conference{
author = "Isaković, Anđelka and Jeremić, Marija and Krstić, Danijela and Čolović, Mirjana and Kortz, Ulrich and Misirlić-Denčić, Sonja",
year = "2021",
abstract = "Background: Polyoxometalates are a class of anionic, polynuclear metal-oxo clusters reported as promising in vitro and in vivo antitumor agents for several decades. The aim of this study was to investigate the antineuroblastoma potential of the polyoxopalladate(II) nanocube Na8[Pd13As8O34(OH)6]・42H2O (Pd13). Material and methods: All experiments were performed on human neuroblastoma cell line, SH-SY5Y. The number of viable cells after the treatment with Pd13 was assessed using an acid phosphatase viability assay. The level of superoxide ion, mitochondrial membrane potential, pan-caspase activity, acidic intracellular vesicles content, and the cell cycle was determined by flow cytometry. Results: The obtained results suggest that Pd13 caused a significant decrease in cell viability with IC50 values of 7.7 µM (24 h) and 4.4 µM (48 h). Pd13 induced depolarization of mitochondrial membrane (2 h), followed by ~ 30% increase in the production of the superoxide ion (O2-) 4 h after treatment. An increase (~ 30%) in pancaspase activation and disturbance of neuroblastma cell cycle were observed after 24 h treatment. Namely, Pd13 caused an increase (14.4%) in the number of cells with fragmented nuclear DNA (SubG0), a decrease (%) of cells in the G1 phase, and an increase (%) in the S phase, all suggestive of cell cycle arrest. Finally, Pd13 increased the orange to green fluorescence ratio for ~ 45% 24 h after treatment, supporting intracellular acidification. Conclusion: The polyoxopalladate, Pd13 can be regarded as a promising antineuroblastoma agent which induces oxidative stress, and causes pan-caspase activation, DNA fragmentation and cell cycle arrest, which are all hallmarks of apoptotic neuroblastoma cell death.",
publisher = "Belgrade : Serbian Association for Cancer Research (SDIR)",
journal = "SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts",
title = "Antineuroblastoma potential of polyoxopalladate(II)",
pages = "69-69",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12964"
}

Isaković, A., Jeremić, M., Krstić, D., Čolović, M., Kortz, U.,& Misirlić-Denčić, S.. (2021). Antineuroblastoma potential of polyoxopalladate(II). in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts
Belgrade : Serbian Association for Cancer Research (SDIR)., 69-69.
https://hdl.handle.net/21.15107/rcub_vinar_12964

Isaković A, Jeremić M, Krstić D, Čolović M, Kortz U, Misirlić-Denčić S. Antineuroblastoma potential of polyoxopalladate(II). in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts. 2021;:69-69.
https://hdl.handle.net/21.15107/rcub_vinar_12964 .

Isaković, Anđelka, Jeremić, Marija, Krstić, Danijela, Čolović, Mirjana, Kortz, Ulrich, Misirlić-Denčić, Sonja, "Antineuroblastoma potential of polyoxopalladate(II)" in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts (2021):69-69,
https://hdl.handle.net/21.15107/rcub_vinar_12964 .

TY  - CONF
AU  - Jeremić, Marija
AU  - Isaković, Anđelka
AU  - Krstić, Danijela
AU  - Čolović, Mirjana
AU  - Kortz, Ulrich
AU  - Misirlić-Denčić, Sonja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12965
AB  - Background: Polyoxo-noble-metalates, a class of molecular noble metal-oxo nanoclusters that combine features of both polyoxometalates and noble metals, are a promising platform for the development of nextgeneration antitumor metallodrugs. The aim of this study was to evaluate the antineuroblastoma potential of three novel polyoxopalladates. Material and methods: All experiments were performed on human neuroblastoma cell line, SHSY5Y. The three polyoxo-noble-palladates Na4[SrPd12O6(OH)3(PhAsO3)6(OAc)3] ・2NaOAc・32H2O (SrPd12), Na12[SnIVO8Pd12(PO4)8]·43H2O (SnPd12) and Na12[PbIVO8Pd12(PO4)8]·38H2O (PbPd12) were investigated in our study. The viability of neuroblastoma cells after 24h treatment was assessed using an acid phosphatase assay. The level of superoxide ion, mitochondrial membrane potential, pan-caspase activity, cell cycle analysis and acidic vesicles content were determined by flow cytometry using appropriate fluorochromes. Results: Calculated IC50 (μM; 24h) values were 75.8 ± 6.7 (SrPd12) and >>100 (SnPd12 and PbPd12), selecting SrPd12 as the most efficient. SrPd12 did not affect the mitochondrial membrane potential and superoxide production in neuroblastoma cells after short (2 h and 4 h) exposure. Also, it did not induce an increase in the number of neuroblastoma cells with fragmented DNA content, but displayed the cell cycle arrest: the ~ 23% reduction of neuroblastoma cells in G0/G1 phase and the ~ 17% increase in S phase. The treatment with SrPd12 did not increase the level of acidic vesicles but it increased the activity of caspases five-fold. Conclusion: Only SrPd12 exhibited a satisfactory antineuroblastoma action by inducing caspase activation and neuroblastoma cell cycle arrest.
PB  - Belgrade : Serbian Association for Cancer Research (SDIR)
C3  - SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts
T1  - Selected polyoxopalladates as potential antitumor drug candidates
SP  - 70
EP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12965
ER  - 

@conference{
author = "Jeremić, Marija and Isaković, Anđelka and Krstić, Danijela and Čolović, Mirjana and Kortz, Ulrich and Misirlić-Denčić, Sonja",
year = "2021",
abstract = "Background: Polyoxo-noble-metalates, a class of molecular noble metal-oxo nanoclusters that combine features of both polyoxometalates and noble metals, are a promising platform for the development of nextgeneration antitumor metallodrugs. The aim of this study was to evaluate the antineuroblastoma potential of three novel polyoxopalladates. Material and methods: All experiments were performed on human neuroblastoma cell line, SHSY5Y. The three polyoxo-noble-palladates Na4[SrPd12O6(OH)3(PhAsO3)6(OAc)3] ・2NaOAc・32H2O (SrPd12), Na12[SnIVO8Pd12(PO4)8]·43H2O (SnPd12) and Na12[PbIVO8Pd12(PO4)8]·38H2O (PbPd12) were investigated in our study. The viability of neuroblastoma cells after 24h treatment was assessed using an acid phosphatase assay. The level of superoxide ion, mitochondrial membrane potential, pan-caspase activity, cell cycle analysis and acidic vesicles content were determined by flow cytometry using appropriate fluorochromes. Results: Calculated IC50 (μM; 24h) values were 75.8 ± 6.7 (SrPd12) and >>100 (SnPd12 and PbPd12), selecting SrPd12 as the most efficient. SrPd12 did not affect the mitochondrial membrane potential and superoxide production in neuroblastoma cells after short (2 h and 4 h) exposure. Also, it did not induce an increase in the number of neuroblastoma cells with fragmented DNA content, but displayed the cell cycle arrest: the ~ 23% reduction of neuroblastoma cells in G0/G1 phase and the ~ 17% increase in S phase. The treatment with SrPd12 did not increase the level of acidic vesicles but it increased the activity of caspases five-fold. Conclusion: Only SrPd12 exhibited a satisfactory antineuroblastoma action by inducing caspase activation and neuroblastoma cell cycle arrest.",
publisher = "Belgrade : Serbian Association for Cancer Research (SDIR)",
journal = "SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts",
title = "Selected polyoxopalladates as potential antitumor drug candidates",
pages = "70-70",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12965"
}

Jeremić, M., Isaković, A., Krstić, D., Čolović, M., Kortz, U.,& Misirlić-Denčić, S.. (2021). Selected polyoxopalladates as potential antitumor drug candidates. in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts
Belgrade : Serbian Association for Cancer Research (SDIR)., 70-70.
https://hdl.handle.net/21.15107/rcub_vinar_12965

Jeremić M, Isaković A, Krstić D, Čolović M, Kortz U, Misirlić-Denčić S. Selected polyoxopalladates as potential antitumor drug candidates. in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts. 2021;:70-70.
https://hdl.handle.net/21.15107/rcub_vinar_12965 .

Jeremić, Marija, Isaković, Anđelka, Krstić, Danijela, Čolović, Mirjana, Kortz, Ulrich, Misirlić-Denčić, Sonja, "Selected polyoxopalladates as potential antitumor drug candidates" in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts (2021):70-70,
https://hdl.handle.net/21.15107/rcub_vinar_12965 .