TY  - JOUR
AU  - Seke, Mariana
AU  - Markelić, Milica
AU  - Morina, Arian
AU  - Jović, Danica S.
AU  - Korać, Aleksandra
AU  - Miličić, Dragana
AU  - Đorđević, Aleksandar N.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1609
AB  - Adsorption of non-polar compounds by suspended fullerene nanoaggregates (nC(60)) may enhance their toxicity and affect the fate, transformation, and transport of non-polar compounds in the environment. The potential of stable fullerene nanoaggregates as contaminant carriers in aqueous systems and the influence of chloromethanes (trichloromethane and dichloromethane) were studied on the midgut epithelial cells of Daphnia magna by light and electron microscopy. The size and shape of fullerene nanoaggregates were observed and measured using dynamic light scattering, transmission electron microscopy, and low vacuum scanning electron microscopy. The nC(60) in suspension appeared as a bulk of aggregates of irregular shape with a surface consisting of small clumps 20-30 nm in diameter. The presence of nC(60) aggregates was confirmed in midgut lumen and epithelial cells of D. magna. After in vivo acute exposure to chloromethane, light and electron microscopy revealed an extensive cytoplasmic vacuolization with disruption and loss of specific structures of D. magna midgut epithelium (mitochondria, endoplasmic reticulum, microvilli, peritrophic membrane) and increased appearance of necrotic cells. The degree of observed changes depended on the type of treatment: trichloromethane (TCM) induced the most notable changes, whereas fullerene nanoaggregates alone had no negative effects. Transmission electron microscopy also indicated increased lysosomal degradation and severe peroxidative damages of enterocyte mitochondria following combined exposure to chloromethane and fullerene nanoaggregates. In conclusion, the adsorption of chloromethane by fullerene nanoaggregates enhances their toxicity and induces peroxidative mitochondrial damage in midgut enterocytes.
T2  - Protoplasma
T1  - Synergistic mitotoxicity of chloromethanes and fullerene C-60 nanoaggregates in Daphnia magna midgut epithelial cells
VL  - 254
IS  - 4
SP  - 1607
EP  - 1616
DO  - 10.1007/s00709-016-1049-9
ER  - 

@article{
author = "Seke, Mariana and Markelić, Milica and Morina, Arian and Jović, Danica S. and Korać, Aleksandra and Miličić, Dragana and Đorđević, Aleksandar N.",
year = "2017",
abstract = "Adsorption of non-polar compounds by suspended fullerene nanoaggregates (nC(60)) may enhance their toxicity and affect the fate, transformation, and transport of non-polar compounds in the environment. The potential of stable fullerene nanoaggregates as contaminant carriers in aqueous systems and the influence of chloromethanes (trichloromethane and dichloromethane) were studied on the midgut epithelial cells of Daphnia magna by light and electron microscopy. The size and shape of fullerene nanoaggregates were observed and measured using dynamic light scattering, transmission electron microscopy, and low vacuum scanning electron microscopy. The nC(60) in suspension appeared as a bulk of aggregates of irregular shape with a surface consisting of small clumps 20-30 nm in diameter. The presence of nC(60) aggregates was confirmed in midgut lumen and epithelial cells of D. magna. After in vivo acute exposure to chloromethane, light and electron microscopy revealed an extensive cytoplasmic vacuolization with disruption and loss of specific structures of D. magna midgut epithelium (mitochondria, endoplasmic reticulum, microvilli, peritrophic membrane) and increased appearance of necrotic cells. The degree of observed changes depended on the type of treatment: trichloromethane (TCM) induced the most notable changes, whereas fullerene nanoaggregates alone had no negative effects. Transmission electron microscopy also indicated increased lysosomal degradation and severe peroxidative damages of enterocyte mitochondria following combined exposure to chloromethane and fullerene nanoaggregates. In conclusion, the adsorption of chloromethane by fullerene nanoaggregates enhances their toxicity and induces peroxidative mitochondrial damage in midgut enterocytes.",
journal = "Protoplasma",
title = "Synergistic mitotoxicity of chloromethanes and fullerene C-60 nanoaggregates in Daphnia magna midgut epithelial cells",
volume = "254",
number = "4",
pages = "1607-1616",
doi = "10.1007/s00709-016-1049-9"
}

Seke, M., Markelić, M., Morina, A., Jović, D. S., Korać, A., Miličić, D.,& Đorđević, A. N.. (2017). Synergistic mitotoxicity of chloromethanes and fullerene C-60 nanoaggregates in Daphnia magna midgut epithelial cells. in Protoplasma, 254(4), 1607-1616.
https://doi.org/10.1007/s00709-016-1049-9

Seke M, Markelić M, Morina A, Jović DS, Korać A, Miličić D, Đorđević AN. Synergistic mitotoxicity of chloromethanes and fullerene C-60 nanoaggregates in Daphnia magna midgut epithelial cells. in Protoplasma. 2017;254(4):1607-1616.
doi:10.1007/s00709-016-1049-9 .

Seke, Mariana, Markelić, Milica, Morina, Arian, Jović, Danica S., Korać, Aleksandra, Miličić, Dragana, Đorđević, Aleksandar N., "Synergistic mitotoxicity of chloromethanes and fullerene C-60 nanoaggregates in Daphnia magna midgut epithelial cells" in Protoplasma, 254, no. 4 (2017):1607-1616,
https://doi.org/10.1007/s00709-016-1049-9 . .

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Bulat, Tanja M.
AU  - Golic, Igor
AU  - Incerti, Sebastien
AU  - Korać, Aleksandra
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1044
AB  - In most patients with lung cancer radiation treatment is used either as single agent or in combination with radiosensitizing drugs. However, the mechanisms underlying combined therapy and its impact on different modes of cell death have not yet been fully elucidated. We aimed to examine effects of single and combined treatments with gamma-rays and erlotinib on radioresistant CRL-5876 human lung adenocarcinoma cells with particular emphasis on cell death. CRL-5876 cells were treated with gamma-rays and/or erlotinib and changes in cell cycle, DNA repair dynamics, ultrastructure, nuclear morphology and protein expression were monitored at different time points. To reveal the relationship between types of cell death that arise after these treatments, autophagy was blocked with chloroquine. We found that higher dose of gamma-rays causes G2/M arrest while adding of erlotinib to this treatment decreases the number of cells in S phase. Impact of erlotinib on kinetics of disappearance of irradiation-induced DNA double strand breaks is reflected in the increase of residual gamma-H2AX foci after 24 h. gamma-rays provoke cytoprotective autophagy which precedes development of senescence. Erlotinib predominantly induces apoptosis and enlarges the number of apoptotic cells in the irradiated CRL-5876 cells. Chloroquine improved cytotoxicity induced by radiation and erlotinib, increased apoptosis and decreased senescence in the CRL-5876 cells. The results obtained on CRL-5876 cells indicate significant radiosensitizing effect of erlotinib and suggest that chloroquine in the combination with the above treatments may have an additional antitumor effect in lung adenocarcinoma.
T2  - Cell Biology and Toxicology
T1  - The impact of autophagy on cell death modalities in CRL-5876 lung adenocarcinoma cells after their exposure to gamma-rays and/or erlotinib
VL  - 32
IS  - 2
SP  - 83
EP  - 101
DO  - 10.1007/s10565-016-9319-z
ER  - 

@article{
author = "Keta, Otilija D. and Bulat, Tanja M. and Golic, Igor and Incerti, Sebastien and Korać, Aleksandra and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2016",
abstract = "In most patients with lung cancer radiation treatment is used either as single agent or in combination with radiosensitizing drugs. However, the mechanisms underlying combined therapy and its impact on different modes of cell death have not yet been fully elucidated. We aimed to examine effects of single and combined treatments with gamma-rays and erlotinib on radioresistant CRL-5876 human lung adenocarcinoma cells with particular emphasis on cell death. CRL-5876 cells were treated with gamma-rays and/or erlotinib and changes in cell cycle, DNA repair dynamics, ultrastructure, nuclear morphology and protein expression were monitored at different time points. To reveal the relationship between types of cell death that arise after these treatments, autophagy was blocked with chloroquine. We found that higher dose of gamma-rays causes G2/M arrest while adding of erlotinib to this treatment decreases the number of cells in S phase. Impact of erlotinib on kinetics of disappearance of irradiation-induced DNA double strand breaks is reflected in the increase of residual gamma-H2AX foci after 24 h. gamma-rays provoke cytoprotective autophagy which precedes development of senescence. Erlotinib predominantly induces apoptosis and enlarges the number of apoptotic cells in the irradiated CRL-5876 cells. Chloroquine improved cytotoxicity induced by radiation and erlotinib, increased apoptosis and decreased senescence in the CRL-5876 cells. The results obtained on CRL-5876 cells indicate significant radiosensitizing effect of erlotinib and suggest that chloroquine in the combination with the above treatments may have an additional antitumor effect in lung adenocarcinoma.",
journal = "Cell Biology and Toxicology",
title = "The impact of autophagy on cell death modalities in CRL-5876 lung adenocarcinoma cells after their exposure to gamma-rays and/or erlotinib",
volume = "32",
number = "2",
pages = "83-101",
doi = "10.1007/s10565-016-9319-z"
}

Keta, O. D., Bulat, T. M., Golic, I., Incerti, S., Korać, A., Petrović, I. M.,& Ristić-Fira, A.. (2016). The impact of autophagy on cell death modalities in CRL-5876 lung adenocarcinoma cells after their exposure to gamma-rays and/or erlotinib. in Cell Biology and Toxicology, 32(2), 83-101.
https://doi.org/10.1007/s10565-016-9319-z

Keta OD, Bulat TM, Golic I, Incerti S, Korać A, Petrović IM, Ristić-Fira A. The impact of autophagy on cell death modalities in CRL-5876 lung adenocarcinoma cells after their exposure to gamma-rays and/or erlotinib. in Cell Biology and Toxicology. 2016;32(2):83-101.
doi:10.1007/s10565-016-9319-z .

Keta, Otilija D., Bulat, Tanja M., Golic, Igor, Incerti, Sebastien, Korać, Aleksandra, Petrović, Ivan M., Ristić-Fira, Aleksandra, "The impact of autophagy on cell death modalities in CRL-5876 lung adenocarcinoma cells after their exposure to gamma-rays and/or erlotinib" in Cell Biology and Toxicology, 32, no. 2 (2016):83-101,
https://doi.org/10.1007/s10565-016-9319-z . .