TY  - JOUR
AU  - Veljković, Veljko
AU  - Glišić, Sanja
AU  - Muller, Claude P.
AU  - Scotch, Matthew
AU  - Branch, Donald R.
AU  - Perović, Vladimir R.
AU  - Senćanski, Milan V.
AU  - Veljković, Nevena V.
AU  - Colombatti, Alfonso
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/423
AB  - The worst Ebola virus (EV) outbreak in history has hit Liberia, Sierra Leone and Guinea hardest and the trend lines in this crisis are grave, and now represents a global public health threat concern. Limited therapeutic and/or prophylactic options are available for people suffering from Ebola virus disease (EVD) and further complicate the situation. Previous studies suggested that the EV glycoprotein (GP) is the main determinant causing structural damage of endothelial cells that triggers the hemorrhagic diathesis, but molecular mechanisms underlying this phenomenon remains elusive. Using the informational spectrum method (ISM), a virtual spectroscopy method for analysis of the protein-protein interactions, the interaction of GP with endothelial extracellular matrix (ECM) was investigated. Presented results of this in silico study suggest that Elastin Microfibril Interface Located Proteins (EMILINs) are involved in interaction between GP and ECM. This finding could contribute to a better understanding of EV/endothelium interaction and its role in pathogenesis, prevention and therapy of EVD.
T2  - Frontiers in Microbiology
T1  - In silico analysis suggests interaction between Ebola virus and the extracellular matrix
VL  - 6
DO  - 10.3389/fmicb.2015.00135
ER  - 

@article{
author = "Veljković, Veljko and Glišić, Sanja and Muller, Claude P. and Scotch, Matthew and Branch, Donald R. and Perović, Vladimir R. and Senćanski, Milan V. and Veljković, Nevena V. and Colombatti, Alfonso",
year = "2015",
abstract = "The worst Ebola virus (EV) outbreak in history has hit Liberia, Sierra Leone and Guinea hardest and the trend lines in this crisis are grave, and now represents a global public health threat concern. Limited therapeutic and/or prophylactic options are available for people suffering from Ebola virus disease (EVD) and further complicate the situation. Previous studies suggested that the EV glycoprotein (GP) is the main determinant causing structural damage of endothelial cells that triggers the hemorrhagic diathesis, but molecular mechanisms underlying this phenomenon remains elusive. Using the informational spectrum method (ISM), a virtual spectroscopy method for analysis of the protein-protein interactions, the interaction of GP with endothelial extracellular matrix (ECM) was investigated. Presented results of this in silico study suggest that Elastin Microfibril Interface Located Proteins (EMILINs) are involved in interaction between GP and ECM. This finding could contribute to a better understanding of EV/endothelium interaction and its role in pathogenesis, prevention and therapy of EVD.",
journal = "Frontiers in Microbiology",
title = "In silico analysis suggests interaction between Ebola virus and the extracellular matrix",
volume = "6",
doi = "10.3389/fmicb.2015.00135"
}

Veljković, V., Glišić, S., Muller, C. P., Scotch, M., Branch, D. R., Perović, V. R., Senćanski, M. V., Veljković, N. V.,& Colombatti, A.. (2015). In silico analysis suggests interaction between Ebola virus and the extracellular matrix. in Frontiers in Microbiology, 6.
https://doi.org/10.3389/fmicb.2015.00135

Veljković V, Glišić S, Muller CP, Scotch M, Branch DR, Perović VR, Senćanski MV, Veljković NV, Colombatti A. In silico analysis suggests interaction between Ebola virus and the extracellular matrix. in Frontiers in Microbiology. 2015;6.
doi:10.3389/fmicb.2015.00135 .

Veljković, Veljko, Glišić, Sanja, Muller, Claude P., Scotch, Matthew, Branch, Donald R., Perović, Vladimir R., Senćanski, Milan V., Veljković, Nevena V., Colombatti, Alfonso, "In silico analysis suggests interaction between Ebola virus and the extracellular matrix" in Frontiers in Microbiology, 6 (2015),
https://doi.org/10.3389/fmicb.2015.00135 . .

TY  - JOUR
AU  - Veljković, Veljko
AU  - Glišić, Sanja
AU  - Veljković, Nevena V.
AU  - Bojić, Tijana
AU  - Dietrich, Ursula
AU  - Perović, Vladimir R.
AU  - Colombatti, Alfonso
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/229
AB  - Despite plausible evidence for beneficial effects of the vaccination against influenza in cardiovascular diseases (CVD) very limited studies have been carried out to explain the molecular mechanism of this phenomenon. Using the informational spectrum method (ISM), a virtual spectroscopy method for analysis of protein-protein interactions, the bradykinin 2 receptor (BKB2R) was identified as a principal host protein which could mediate molecular processes underlying the cardioprotective effect of influenza vaccines. Based on this finding we suggest that some antibodies elicited by influenza vaccines act as agonists, which activate a BKB2R-associated signaling pathway contributing to the protection against CVD. The ISM analysis of 14 influenza viruses, which were used as components of seasonal vaccines, revealed four vaccine viruses A/Beijing/262/95(H1N1), A/NewCaledonia/20/1999(H1N1), A/Christchurch/28/2003(H3N2) and A/Perth/16/2009(H3N2), which could be suited best for further studies on the cardioprotective effect of influenza vaccines. (C) 2014 Elsevier Ltd. All rights reserved.
T2  - Vaccine
T1  - Influenza vaccine as prevention for cardiovascular diseases: Possible molecular mechanism
VL  - 32
IS  - 48
SP  - 6569
EP  - 6575
DO  - 10.1016/j.vaccine.2014.07.007
ER  - 

@article{
author = "Veljković, Veljko and Glišić, Sanja and Veljković, Nevena V. and Bojić, Tijana and Dietrich, Ursula and Perović, Vladimir R. and Colombatti, Alfonso",
year = "2014",
abstract = "Despite plausible evidence for beneficial effects of the vaccination against influenza in cardiovascular diseases (CVD) very limited studies have been carried out to explain the molecular mechanism of this phenomenon. Using the informational spectrum method (ISM), a virtual spectroscopy method for analysis of protein-protein interactions, the bradykinin 2 receptor (BKB2R) was identified as a principal host protein which could mediate molecular processes underlying the cardioprotective effect of influenza vaccines. Based on this finding we suggest that some antibodies elicited by influenza vaccines act as agonists, which activate a BKB2R-associated signaling pathway contributing to the protection against CVD. The ISM analysis of 14 influenza viruses, which were used as components of seasonal vaccines, revealed four vaccine viruses A/Beijing/262/95(H1N1), A/NewCaledonia/20/1999(H1N1), A/Christchurch/28/2003(H3N2) and A/Perth/16/2009(H3N2), which could be suited best for further studies on the cardioprotective effect of influenza vaccines. (C) 2014 Elsevier Ltd. All rights reserved.",
journal = "Vaccine",
title = "Influenza vaccine as prevention for cardiovascular diseases: Possible molecular mechanism",
volume = "32",
number = "48",
pages = "6569-6575",
doi = "10.1016/j.vaccine.2014.07.007"
}

Veljković, V., Glišić, S., Veljković, N. V., Bojić, T., Dietrich, U., Perović, V. R.,& Colombatti, A.. (2014). Influenza vaccine as prevention for cardiovascular diseases: Possible molecular mechanism. in Vaccine, 32(48), 6569-6575.
https://doi.org/10.1016/j.vaccine.2014.07.007

Veljković V, Glišić S, Veljković NV, Bojić T, Dietrich U, Perović VR, Colombatti A. Influenza vaccine as prevention for cardiovascular diseases: Possible molecular mechanism. in Vaccine. 2014;32(48):6569-6575.
doi:10.1016/j.vaccine.2014.07.007 .

Veljković, Veljko, Glišić, Sanja, Veljković, Nevena V., Bojić, Tijana, Dietrich, Ursula, Perović, Vladimir R., Colombatti, Alfonso, "Influenza vaccine as prevention for cardiovascular diseases: Possible molecular mechanism" in Vaccine, 32, no. 48 (2014):6569-6575,
https://doi.org/10.1016/j.vaccine.2014.07.007 . .

TY  - JOUR
AU  - Veljković, Milena
AU  - Dopsaj, Violeta
AU  - Dopsaj, Milivoj
AU  - Branch, Donald R.
AU  - Veljković, Nevena V.
AU  - Sakarellos-Daitsiotis, Maria M.
AU  - Veljković, Veljko
AU  - Glišić, Sanja
AU  - Colombatti, Alfonso
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4613
AB  - Background: There is convincing evidence from numerous clinical and epidemiological studies that physical activity can reduce the risk for breast and prostate cancer. The biological mechanisms underlying this phenomenon remain elusive. Herein we suggest a role for naturally produced antibodies reactive with the vasoactive intestinal peptide (VIP) in the suppression of breast and prostate cancer, which we believe could offer a possible molecular mechanism underlying control of these cancers by physical exercise. Methodology and Results: We found that sera from individuals having breast and prostate cancers have decreased titers of VIP natural antibodies as demonstrated by a lower reactivity against peptide NTM1, having similar informational and structural properties as VIP. In contrast, sera collected from elite athletes, exhibited titers of natural NTM1-reactive antibodies that are significantly increased, suggesting that physical activity boosts production of these antibodies. Significance: Presented results suggest that physical exercise stimulates production of natural anti-VIP antibodies and likely results in suppression of VIP. This, in turn, may play a protective role against breast and prostate cancers. Physical exercise should be further investigated as a potential tool in the treatment of these diseases.
T2  - PLOS One
T1  - Physical Activity and Natural Anti-VIP Antibodies: Potential Role in Breast and Prostate Cancer Therapy
VL  - 6
IS  - 11
DO  - 10.1371/journal.pone.0028304
ER  - 

@article{
author = "Veljković, Milena and Dopsaj, Violeta and Dopsaj, Milivoj and Branch, Donald R. and Veljković, Nevena V. and Sakarellos-Daitsiotis, Maria M. and Veljković, Veljko and Glišić, Sanja and Colombatti, Alfonso",
year = "2011",
abstract = "Background: There is convincing evidence from numerous clinical and epidemiological studies that physical activity can reduce the risk for breast and prostate cancer. The biological mechanisms underlying this phenomenon remain elusive. Herein we suggest a role for naturally produced antibodies reactive with the vasoactive intestinal peptide (VIP) in the suppression of breast and prostate cancer, which we believe could offer a possible molecular mechanism underlying control of these cancers by physical exercise. Methodology and Results: We found that sera from individuals having breast and prostate cancers have decreased titers of VIP natural antibodies as demonstrated by a lower reactivity against peptide NTM1, having similar informational and structural properties as VIP. In contrast, sera collected from elite athletes, exhibited titers of natural NTM1-reactive antibodies that are significantly increased, suggesting that physical activity boosts production of these antibodies. Significance: Presented results suggest that physical exercise stimulates production of natural anti-VIP antibodies and likely results in suppression of VIP. This, in turn, may play a protective role against breast and prostate cancers. Physical exercise should be further investigated as a potential tool in the treatment of these diseases.",
journal = "PLOS One",
title = "Physical Activity and Natural Anti-VIP Antibodies: Potential Role in Breast and Prostate Cancer Therapy",
volume = "6",
number = "11",
doi = "10.1371/journal.pone.0028304"
}

Veljković, M., Dopsaj, V., Dopsaj, M., Branch, D. R., Veljković, N. V., Sakarellos-Daitsiotis, M. M., Veljković, V., Glišić, S.,& Colombatti, A.. (2011). Physical Activity and Natural Anti-VIP Antibodies: Potential Role in Breast and Prostate Cancer Therapy. in PLOS One, 6(11).
https://doi.org/10.1371/journal.pone.0028304

Veljković M, Dopsaj V, Dopsaj M, Branch DR, Veljković NV, Sakarellos-Daitsiotis MM, Veljković V, Glišić S, Colombatti A. Physical Activity and Natural Anti-VIP Antibodies: Potential Role in Breast and Prostate Cancer Therapy. in PLOS One. 2011;6(11).
doi:10.1371/journal.pone.0028304 .

Veljković, Milena, Dopsaj, Violeta, Dopsaj, Milivoj, Branch, Donald R., Veljković, Nevena V., Sakarellos-Daitsiotis, Maria M., Veljković, Veljko, Glišić, Sanja, Colombatti, Alfonso, "Physical Activity and Natural Anti-VIP Antibodies: Potential Role in Breast and Prostate Cancer Therapy" in PLOS One, 6, no. 11 (2011),
https://doi.org/10.1371/journal.pone.0028304 . .

TY  - JOUR
AU  - Veljković, Milena
AU  - Branch, Donald R.
AU  - Dopsaj, Violeta
AU  - Veljković, Veljko
AU  - Veljković, Nevena V.
AU  - Glišić, Sanja
AU  - Colombatti, Alfonso
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4495
AB  - Objectives: The incidence of non-AIDS-defining cancer is remarkably higher in HIV-infected than in the general population. In contrast, breast cancer risk is significantly reduced in the HIV-infected population. The molecular mechanisms underlying the phenomenon of suppression of breast cancer in the HIV-infected population may serve as a basis for development of a new platform for prevention and treatment of breast cancer. Hypothesis: Various evidences indicate that vasoactive intestinal peptide (VIP) plays an important role in growth, and differentiation of breast cancer. We previously showed (i) that natural antibodies recognizing VIP and the gp120-derived peptide NTM significantly contribute to the control of HIV disease progression by suppression of VIP-like activity of HIV-1 gp120 and (ii) that physical exercise stimulates production of these natural antibodies. These findings suggest that natural anti-VIP/NTM antibodies could contribute to a decrease of breast cancer in the HIV-infected population by suppression of VIP, which may play a pro/oncogenic function. Aerobic exercise which stimulates production of anti-VIP/NTM antibodies could be used as prevention and supportive treatment of breast cancer. Impact: Immunotherapy based on natural anti-VIP/NTM antibodies could serve as an effective adjunct therapy for the treatment of breast cancer. Similarly, aerobic exercise, which stimulates production of these antibodies, should be considered as an inexpensive and safe preventive and supportive breast cancer therapy. Natural anti-VIP/NTM antibodies also represent promising prognostic marker for breast cancer. (C) 2011 Elsevier Ltd. All rights reserved.
T2  - Medical Hypotheses
T1  - Can natural antibodies to VIP or VIP-like HIV-1 glycoprotein facilitate prevention and supportive treatment of breast cancer?
VL  - 77
IS  - 3
SP  - 404
EP  - 408
DO  - 10.1016/j.mehy.2011.05.030
ER  - 

@article{
author = "Veljković, Milena and Branch, Donald R. and Dopsaj, Violeta and Veljković, Veljko and Veljković, Nevena V. and Glišić, Sanja and Colombatti, Alfonso",
year = "2011",
abstract = "Objectives: The incidence of non-AIDS-defining cancer is remarkably higher in HIV-infected than in the general population. In contrast, breast cancer risk is significantly reduced in the HIV-infected population. The molecular mechanisms underlying the phenomenon of suppression of breast cancer in the HIV-infected population may serve as a basis for development of a new platform for prevention and treatment of breast cancer. Hypothesis: Various evidences indicate that vasoactive intestinal peptide (VIP) plays an important role in growth, and differentiation of breast cancer. We previously showed (i) that natural antibodies recognizing VIP and the gp120-derived peptide NTM significantly contribute to the control of HIV disease progression by suppression of VIP-like activity of HIV-1 gp120 and (ii) that physical exercise stimulates production of these natural antibodies. These findings suggest that natural anti-VIP/NTM antibodies could contribute to a decrease of breast cancer in the HIV-infected population by suppression of VIP, which may play a pro/oncogenic function. Aerobic exercise which stimulates production of anti-VIP/NTM antibodies could be used as prevention and supportive treatment of breast cancer. Impact: Immunotherapy based on natural anti-VIP/NTM antibodies could serve as an effective adjunct therapy for the treatment of breast cancer. Similarly, aerobic exercise, which stimulates production of these antibodies, should be considered as an inexpensive and safe preventive and supportive breast cancer therapy. Natural anti-VIP/NTM antibodies also represent promising prognostic marker for breast cancer. (C) 2011 Elsevier Ltd. All rights reserved.",
journal = "Medical Hypotheses",
title = "Can natural antibodies to VIP or VIP-like HIV-1 glycoprotein facilitate prevention and supportive treatment of breast cancer?",
volume = "77",
number = "3",
pages = "404-408",
doi = "10.1016/j.mehy.2011.05.030"
}

Veljković, M., Branch, D. R., Dopsaj, V., Veljković, V., Veljković, N. V., Glišić, S.,& Colombatti, A.. (2011). Can natural antibodies to VIP or VIP-like HIV-1 glycoprotein facilitate prevention and supportive treatment of breast cancer?. in Medical Hypotheses, 77(3), 404-408.
https://doi.org/10.1016/j.mehy.2011.05.030

Veljković M, Branch DR, Dopsaj V, Veljković V, Veljković NV, Glišić S, Colombatti A. Can natural antibodies to VIP or VIP-like HIV-1 glycoprotein facilitate prevention and supportive treatment of breast cancer?. in Medical Hypotheses. 2011;77(3):404-408.
doi:10.1016/j.mehy.2011.05.030 .

Veljković, Milena, Branch, Donald R., Dopsaj, Violeta, Veljković, Veljko, Veljković, Nevena V., Glišić, Sanja, Colombatti, Alfonso, "Can natural antibodies to VIP or VIP-like HIV-1 glycoprotein facilitate prevention and supportive treatment of breast cancer?" in Medical Hypotheses, 77, no. 3 (2011):404-408,
https://doi.org/10.1016/j.mehy.2011.05.030 . .

TY  - JOUR
AU  - Doliana, Roberto
AU  - Veljković, Veljko
AU  - Prljić, Jelena
AU  - Veljković, Nevena V.
AU  - De Lorenzo, Elisa
AU  - Mongiat, Maurizio
AU  - Ligresti, Giovanni
AU  - Marastoni, Stefano
AU  - Colombatti, Alfonso
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3377
AB  - The informational spectrum method (ISM) is a virtual spectroscopy method for the fast analysis of potential protein-protein relationships. By applying the ISM approach to the GeneBank protein database the vascular proteins EMILIN1 (Elastin Microfibril Interface Located ProteIN), EMILIN2, MMN1, and MMN2 were identified as additional anthrax PA antigen interacting molecules. This virtual molecular interaction was formally proven by solid phase assays using recombinant proteins. The interaction is independent of the presence of divalent cations and does not involve PA aspartic residue at 683, a critical residue in receptor binding. In fact, the D683A point mutation fully prevented the cell intoxication ability of PA in the presence of Lethal Factor, but it was fully ineffective on the binding of mutated PA to EMILIN1 and EMILIN2. The ISM approach also led to the identification of the potential interaction sites between PA and EMILINs. A PA mutant with a deletion at residue D425 and solid phase protein-protein interaction studies as well as deletion mutant of EMILIN2 confirmed the hypothesized interaction site. Our findings imply that the PA-cell surface receptor interaction is not likely to provide the full explanation for the vascular lesions and prominent hemorrhages that follow Bacillus anthracis infection and spreading and call into play vascular associated proteins such as EMILINs as potential inhibitory proteins. (c) 2007 Elsevier B.V/International Society of Matrix Biology. All rights reserved.
T2  - Matrix Biology
T1  - EMILINs Interact with Anthrax Protective Antigen and Inhibit Toxin Action in Vitro
VL  - 27
IS  - 2
SP  - 96
EP  - 106
DO  - 10.1016/j.matbio.2007.09.008
ER  - 

@article{
author = "Doliana, Roberto and Veljković, Veljko and Prljić, Jelena and Veljković, Nevena V. and De Lorenzo, Elisa and Mongiat, Maurizio and Ligresti, Giovanni and Marastoni, Stefano and Colombatti, Alfonso",
year = "2008",
abstract = "The informational spectrum method (ISM) is a virtual spectroscopy method for the fast analysis of potential protein-protein relationships. By applying the ISM approach to the GeneBank protein database the vascular proteins EMILIN1 (Elastin Microfibril Interface Located ProteIN), EMILIN2, MMN1, and MMN2 were identified as additional anthrax PA antigen interacting molecules. This virtual molecular interaction was formally proven by solid phase assays using recombinant proteins. The interaction is independent of the presence of divalent cations and does not involve PA aspartic residue at 683, a critical residue in receptor binding. In fact, the D683A point mutation fully prevented the cell intoxication ability of PA in the presence of Lethal Factor, but it was fully ineffective on the binding of mutated PA to EMILIN1 and EMILIN2. The ISM approach also led to the identification of the potential interaction sites between PA and EMILINs. A PA mutant with a deletion at residue D425 and solid phase protein-protein interaction studies as well as deletion mutant of EMILIN2 confirmed the hypothesized interaction site. Our findings imply that the PA-cell surface receptor interaction is not likely to provide the full explanation for the vascular lesions and prominent hemorrhages that follow Bacillus anthracis infection and spreading and call into play vascular associated proteins such as EMILINs as potential inhibitory proteins. (c) 2007 Elsevier B.V/International Society of Matrix Biology. All rights reserved.",
journal = "Matrix Biology",
title = "EMILINs Interact with Anthrax Protective Antigen and Inhibit Toxin Action in Vitro",
volume = "27",
number = "2",
pages = "96-106",
doi = "10.1016/j.matbio.2007.09.008"
}

Doliana, R., Veljković, V., Prljić, J., Veljković, N. V., De Lorenzo, E., Mongiat, M., Ligresti, G., Marastoni, S.,& Colombatti, A.. (2008). EMILINs Interact with Anthrax Protective Antigen and Inhibit Toxin Action in Vitro. in Matrix Biology, 27(2), 96-106.
https://doi.org/10.1016/j.matbio.2007.09.008

Doliana R, Veljković V, Prljić J, Veljković NV, De Lorenzo E, Mongiat M, Ligresti G, Marastoni S, Colombatti A. EMILINs Interact with Anthrax Protective Antigen and Inhibit Toxin Action in Vitro. in Matrix Biology. 2008;27(2):96-106.
doi:10.1016/j.matbio.2007.09.008 .

Doliana, Roberto, Veljković, Veljko, Prljić, Jelena, Veljković, Nevena V., De Lorenzo, Elisa, Mongiat, Maurizio, Ligresti, Giovanni, Marastoni, Stefano, Colombatti, Alfonso, "EMILINs Interact with Anthrax Protective Antigen and Inhibit Toxin Action in Vitro" in Matrix Biology, 27, no. 2 (2008):96-106,
https://doi.org/10.1016/j.matbio.2007.09.008 . .