TY  - JOUR
AU  - Jovanović, Predrag
AU  - Borka Jovanović, Vesna
AU  - Borka, Duško
AU  - Zakharov, Alexander F.
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13024
AB  - Here we study possible improvements of the existing constraints on the upper bound of the graviton mass by the analysis of the stellar orbits around the supermassive black hole (SMBH) at the Galactic Center (GC) in the framework of Yukawa gravity. A motivation for this study is a recent detection of Schwarzschild precession in the orbit of S2 star around the SMBH at the GC by the GRAVITY Collaboration. The authors indicated that the orbital precession of the S2 star is close to the general relativity (GR) prediction, but with possible small deviation from it, and parametrized this effect by introducing an ad hoc factor in the parametrized post-Newtonian (PPN) equations of motion. Here we use the value of this factor presented by GRAVITY in order to perform two-body simulations of the stellar orbits in massive gravity using equations of motion in the modified PPN formalism, as well as to constrain the range of massive interaction Λ. From the obtained values of Λ, and assuming that it corresponds to the Compton wavelength of graviton, we then calculated new estimates for the upper bound of graviton mass which are found to be independent, but consistent with the LIGO’s estimate of graviton mass from the first gravitational wave (GW) signal GW150914 (later this graviton mass estimation was significantly improved with consequent observations of GW events). We also performed calculations including numerical simulations in order to constrain the bounds on graviton mass in the case of a small deviation of the stellar orbits from the corresponding GR predictions and showed that our method could further improve previous estimates for the upper bounds on the graviton mass. It is also demonstrated that such an analysis of the observed orbits of S-stars around the GC in the frame of the Yukawa gravity represents a tool for constraining the upper bound for the graviton mass, as well as for probing the predictions of GR or other gravity theories.
T2  - Physical Review D
T1  - Improvement of graviton mass constraints using GRAVITY’s detection of Schwarzschild precession in the orbit of S2 star around the Galactic Center
VL  - 109
IS  - 6
SP  - 064046
DO  - 10.1103/PhysRevD.109.064046
ER  - 

@article{
author = "Jovanović, Predrag and Borka Jovanović, Vesna and Borka, Duško and Zakharov, Alexander F.",
year = "2024",
abstract = "Here we study possible improvements of the existing constraints on the upper bound of the graviton mass by the analysis of the stellar orbits around the supermassive black hole (SMBH) at the Galactic Center (GC) in the framework of Yukawa gravity. A motivation for this study is a recent detection of Schwarzschild precession in the orbit of S2 star around the SMBH at the GC by the GRAVITY Collaboration. The authors indicated that the orbital precession of the S2 star is close to the general relativity (GR) prediction, but with possible small deviation from it, and parametrized this effect by introducing an ad hoc factor in the parametrized post-Newtonian (PPN) equations of motion. Here we use the value of this factor presented by GRAVITY in order to perform two-body simulations of the stellar orbits in massive gravity using equations of motion in the modified PPN formalism, as well as to constrain the range of massive interaction Λ. From the obtained values of Λ, and assuming that it corresponds to the Compton wavelength of graviton, we then calculated new estimates for the upper bound of graviton mass which are found to be independent, but consistent with the LIGO’s estimate of graviton mass from the first gravitational wave (GW) signal GW150914 (later this graviton mass estimation was significantly improved with consequent observations of GW events). We also performed calculations including numerical simulations in order to constrain the bounds on graviton mass in the case of a small deviation of the stellar orbits from the corresponding GR predictions and showed that our method could further improve previous estimates for the upper bounds on the graviton mass. It is also demonstrated that such an analysis of the observed orbits of S-stars around the GC in the frame of the Yukawa gravity represents a tool for constraining the upper bound for the graviton mass, as well as for probing the predictions of GR or other gravity theories.",
journal = "Physical Review D",
title = "Improvement of graviton mass constraints using GRAVITY’s detection of Schwarzschild precession in the orbit of S2 star around the Galactic Center",
volume = "109",
number = "6",
pages = "064046",
doi = "10.1103/PhysRevD.109.064046"
}

Jovanović, P., Borka Jovanović, V., Borka, D.,& Zakharov, A. F.. (2024). Improvement of graviton mass constraints using GRAVITY’s detection of Schwarzschild precession in the orbit of S2 star around the Galactic Center. in Physical Review D, 109(6), 064046.
https://doi.org/10.1103/PhysRevD.109.064046

Jovanović P, Borka Jovanović V, Borka D, Zakharov AF. Improvement of graviton mass constraints using GRAVITY’s detection of Schwarzschild precession in the orbit of S2 star around the Galactic Center. in Physical Review D. 2024;109(6):064046.
doi:10.1103/PhysRevD.109.064046 .

Jovanović, Predrag, Borka Jovanović, Vesna, Borka, Duško, Zakharov, Alexander F., "Improvement of graviton mass constraints using GRAVITY’s detection of Schwarzschild precession in the orbit of S2 star around the Galactic Center" in Physical Review D, 109, no. 6 (2024):064046,
https://doi.org/10.1103/PhysRevD.109.064046 . .

TY  - JOUR
AU  - Borka-Jovanović, Vesna
AU  - Borka, Duško
AU  - Jovanović, Predrag
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12725
AB  - Here we investigate the giant radio galaxy DA 240, which is a FR II source. Specifically, we investigate its flux density, as well as the spectral index distribution. For that purpose, we used publicly available data for the source: Leahy's atlas of double radio-sources and NASA/IPAC Extragalactic Database (NED). We used observations at 326 MHz (92 cm) and at 608 MHz (49 cm) and obtained spectral index distributions between 326 and 608 MHz. For the first time we give spectral index map for these frequencies. We found that the synchrotron radiation is the dominant radiation mechanism over most of the area of DA 240, and also investigated the mechanism of radiation at some characteristic points, namely its core and the hotspots. The results of this study will be helpful for understanding the evolutionary process of the DA 240 radio source.
T2  - Contributions of the Astronomical Observatory Skalnaté Pleso
T1  - Study of spectral index of giant radio galaxy from Leahy's Atlas: DA 240
VL  - 53
IS  - 3
SP  - 188
EP  - 196
DO  - 10.31577/caosp.2023.53.3.188
ER  - 

@article{
author = "Borka-Jovanović, Vesna and Borka, Duško and Jovanović, Predrag",
year = "2023",
abstract = "Here we investigate the giant radio galaxy DA 240, which is a FR II source. Specifically, we investigate its flux density, as well as the spectral index distribution. For that purpose, we used publicly available data for the source: Leahy's atlas of double radio-sources and NASA/IPAC Extragalactic Database (NED). We used observations at 326 MHz (92 cm) and at 608 MHz (49 cm) and obtained spectral index distributions between 326 and 608 MHz. For the first time we give spectral index map for these frequencies. We found that the synchrotron radiation is the dominant radiation mechanism over most of the area of DA 240, and also investigated the mechanism of radiation at some characteristic points, namely its core and the hotspots. The results of this study will be helpful for understanding the evolutionary process of the DA 240 radio source.",
journal = "Contributions of the Astronomical Observatory Skalnaté Pleso",
title = "Study of spectral index of giant radio galaxy from Leahy's Atlas: DA 240",
volume = "53",
number = "3",
pages = "188-196",
doi = "10.31577/caosp.2023.53.3.188"
}

Borka-Jovanović, V., Borka, D.,& Jovanović, P.. (2023). Study of spectral index of giant radio galaxy from Leahy's Atlas: DA 240. in Contributions of the Astronomical Observatory Skalnaté Pleso, 53(3), 188-196.
https://doi.org/10.31577/caosp.2023.53.3.188

Borka-Jovanović V, Borka D, Jovanović P. Study of spectral index of giant radio galaxy from Leahy's Atlas: DA 240. in Contributions of the Astronomical Observatory Skalnaté Pleso. 2023;53(3):188-196.
doi:10.31577/caosp.2023.53.3.188 .

Borka-Jovanović, Vesna, Borka, Duško, Jovanović, Predrag, "Study of spectral index of giant radio galaxy from Leahy's Atlas: DA 240" in Contributions of the Astronomical Observatory Skalnaté Pleso, 53, no. 3 (2023):188-196,
https://doi.org/10.31577/caosp.2023.53.3.188 . .

TY  - CONF
AU  - Borka Jovanović, Vesna
AU  - Borka, Duško
AU  - Jovanović, Predrag
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11941
AB  - Here we study the flux densities distribution at 1380, 4908 and 15365 MHz, as well as the radio spectral index of 3C 84, a Double Radio source Associated with Galactic Nucleus (DRAGN). 3C 84 is the dominant giant elliptical galaxy in the Perseus cluster, and thus very interesting for our research. This famous radio galaxy Perseus A has Relaxed Double classification because it has the large halo, with the lack of its compact structure. We show its structure (using 2D and 3D flux density plot). Besides, we calculated the radio spectral index, which we then used to investigate the nature and mechanisms of its radiation. The obtained values of spectral indices indicated that the northern hotspot is dominated by synchrotron radiation, while in southern hotspot there are thermal and non-thermal radiations, depending on the studied frequency pairs.
C3  - Proceedings of Science
T1  - Flux densities and spectral indices of Relaxed Double radio galaxy 3C 84
VL  - 427
SP  - 193246
DO  - 10.22323/1.427.0043
ER  - 

@conference{
author = "Borka Jovanović, Vesna and Borka, Duško and Jovanović, Predrag",
year = "2023",
abstract = "Here we study the flux densities distribution at 1380, 4908 and 15365 MHz, as well as the radio spectral index of 3C 84, a Double Radio source Associated with Galactic Nucleus (DRAGN). 3C 84 is the dominant giant elliptical galaxy in the Perseus cluster, and thus very interesting for our research. This famous radio galaxy Perseus A has Relaxed Double classification because it has the large halo, with the lack of its compact structure. We show its structure (using 2D and 3D flux density plot). Besides, we calculated the radio spectral index, which we then used to investigate the nature and mechanisms of its radiation. The obtained values of spectral indices indicated that the northern hotspot is dominated by synchrotron radiation, while in southern hotspot there are thermal and non-thermal radiations, depending on the studied frequency pairs.",
journal = "Proceedings of Science",
title = "Flux densities and spectral indices of Relaxed Double radio galaxy 3C 84",
volume = "427",
pages = "193246",
doi = "10.22323/1.427.0043"
}

Borka Jovanović, V., Borka, D.,& Jovanović, P.. (2023). Flux densities and spectral indices of Relaxed Double radio galaxy 3C 84. in Proceedings of Science, 427, 193246.
https://doi.org/10.22323/1.427.0043

Borka Jovanović V, Borka D, Jovanović P. Flux densities and spectral indices of Relaxed Double radio galaxy 3C 84. in Proceedings of Science. 2023;427:193246.
doi:10.22323/1.427.0043 .

Borka Jovanović, Vesna, Borka, Duško, Jovanović, Predrag, "Flux densities and spectral indices of Relaxed Double radio galaxy 3C 84" in Proceedings of Science, 427 (2023):193246,
https://doi.org/10.22323/1.427.0043 . .

TY  - JOUR
AU  - Jovanović, Predrag
AU  - Borka Jovanović, Vesna
AU  - Borka, Duško
AU  - Zakharov, Alexander F.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10849
AB  - In this paper we investigate a Yukawa gravity modification of the Newtonian gravitational potential in a weak field approximation. For that purpose we derived the corresponding equations of motion and used them to perform two-body simulations of the stellar orbits. In 2020 the GRAVITY Collaboration detected the orbital precession of the S2 star around the supermassive black hole (SMBH) at the Galactic Center (GC) and showed that it is close to the general relativity (GR) prediction. Using this observational fact, we evaluated parameters of the Yukawa gravity (the range of Yukawa interaction Λ and universal constant δ) with the Schwarzschild precession of the S-stars assuming that the observed values as indicated by the GRAVITY Collaboration will have a small deviation from GR prediction [1]. GR provides the most natural way to fit observational data for S-star orbits, however, their precessions can be fitted by Yukawa gravity. Our main goal was to study the possible influence of the strength of Yukawa interaction, i.e. the universal constant δ, on the precessions of S-star orbits. We analyze S-star orbits assuming different strength of Yukawa interaction δ and find that this parameter has strong influence on range of Yukawa interaction Λ. For that purpose we use parameterized post-Newtonian (PPN) equations of motion in order to calculate the simulated orbits of S-stars in GR and Yukawa gravity. Using MCMC simulations we obtain the best-fit values and uncertainties of Yukawa gravity parameters for S-stars. Also, we introduce a new criterion which can be used for classification of gravitational systems in this type of gravity, according to their scales. We demonstrated that performed analysis of the observed S-stars orbits around the GC in the frame of the Yukawa gravity represent a tool for constraining the Yukawa gravity parameters and probing the predictions of gravity theories.
T2  - Journal of Cosmology and Astroparticle Physics
T1  - Constraints on Yukawa gravity parameters from observations of bright stars
IS  - 03
SP  - 056
DO  - 10.1088/1475-7516/2023/03/056
ER  - 

@article{
author = "Jovanović, Predrag and Borka Jovanović, Vesna and Borka, Duško and Zakharov, Alexander F.",
year = "2023",
abstract = "In this paper we investigate a Yukawa gravity modification of the Newtonian gravitational potential in a weak field approximation. For that purpose we derived the corresponding equations of motion and used them to perform two-body simulations of the stellar orbits. In 2020 the GRAVITY Collaboration detected the orbital precession of the S2 star around the supermassive black hole (SMBH) at the Galactic Center (GC) and showed that it is close to the general relativity (GR) prediction. Using this observational fact, we evaluated parameters of the Yukawa gravity (the range of Yukawa interaction Λ and universal constant δ) with the Schwarzschild precession of the S-stars assuming that the observed values as indicated by the GRAVITY Collaboration will have a small deviation from GR prediction [1]. GR provides the most natural way to fit observational data for S-star orbits, however, their precessions can be fitted by Yukawa gravity. Our main goal was to study the possible influence of the strength of Yukawa interaction, i.e. the universal constant δ, on the precessions of S-star orbits. We analyze S-star orbits assuming different strength of Yukawa interaction δ and find that this parameter has strong influence on range of Yukawa interaction Λ. For that purpose we use parameterized post-Newtonian (PPN) equations of motion in order to calculate the simulated orbits of S-stars in GR and Yukawa gravity. Using MCMC simulations we obtain the best-fit values and uncertainties of Yukawa gravity parameters for S-stars. Also, we introduce a new criterion which can be used for classification of gravitational systems in this type of gravity, according to their scales. We demonstrated that performed analysis of the observed S-stars orbits around the GC in the frame of the Yukawa gravity represent a tool for constraining the Yukawa gravity parameters and probing the predictions of gravity theories.",
journal = "Journal of Cosmology and Astroparticle Physics",
title = "Constraints on Yukawa gravity parameters from observations of bright stars",
number = "03",
pages = "056",
doi = "10.1088/1475-7516/2023/03/056"
}

Jovanović, P., Borka Jovanović, V., Borka, D.,& Zakharov, A. F.. (2023). Constraints on Yukawa gravity parameters from observations of bright stars. in Journal of Cosmology and Astroparticle Physics(03), 056.
https://doi.org/10.1088/1475-7516/2023/03/056

Jovanović P, Borka Jovanović V, Borka D, Zakharov AF. Constraints on Yukawa gravity parameters from observations of bright stars. in Journal of Cosmology and Astroparticle Physics. 2023;(03):056.
doi:10.1088/1475-7516/2023/03/056 .

Jovanović, Predrag, Borka Jovanović, Vesna, Borka, Duško, Zakharov, Alexander F., "Constraints on Yukawa gravity parameters from observations of bright stars" in Journal of Cosmology and Astroparticle Physics, no. 03 (2023):056,
https://doi.org/10.1088/1475-7516/2023/03/056 . .

TY  - JOUR
AU  - Borka, Duško
AU  - Borka Jovanović, Vesna
AU  - Jovanović, Predrag
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11358
AB  - In this study we used the observed perihelion precession of planetary orbits in the Solar System in order to constrain theory of Yukawa-type gravity, and to bound mass of graviton. For that purpose we assumed that the precession angles of the planets in Yukawa-type gravity should be equal to their observed values, as well as to the corresponding predictions of General Relativity (GR). Starting from these requests we constrain Yukawa-like gravitational potential. The obtained results showed that our estimates for the range of Yukawa interaction Λ at the Solar System scales are in good agreement with recent experimental constrains. Assuming that the estimated value of the range of Λ corresponds to the Compton wavelength of graviton λg, we then estimate the upper bound for its mass mg. We found that these estimates were in agreement with our previous results obtained from the observed stellar orbits around the Galactic Center (GC). © 2023, University of Nis. All rights reserved.
T2  - Filomat
T1  - Bounds on graviton mass and constraining Yukawa-like gravitational potential from planetary motion in the Solar System
VL  - 37
IS  - 25
SP  - 8591
EP  - 8601
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11358
ER  - 

@article{
author = "Borka, Duško and Borka Jovanović, Vesna and Jovanović, Predrag",
year = "2023",
abstract = "In this study we used the observed perihelion precession of planetary orbits in the Solar System in order to constrain theory of Yukawa-type gravity, and to bound mass of graviton. For that purpose we assumed that the precession angles of the planets in Yukawa-type gravity should be equal to their observed values, as well as to the corresponding predictions of General Relativity (GR). Starting from these requests we constrain Yukawa-like gravitational potential. The obtained results showed that our estimates for the range of Yukawa interaction Λ at the Solar System scales are in good agreement with recent experimental constrains. Assuming that the estimated value of the range of Λ corresponds to the Compton wavelength of graviton λg, we then estimate the upper bound for its mass mg. We found that these estimates were in agreement with our previous results obtained from the observed stellar orbits around the Galactic Center (GC). © 2023, University of Nis. All rights reserved.",
journal = "Filomat",
title = "Bounds on graviton mass and constraining Yukawa-like gravitational potential from planetary motion in the Solar System",
volume = "37",
number = "25",
pages = "8591-8601",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11358"
}

Borka, D., Borka Jovanović, V.,& Jovanović, P.. (2023). Bounds on graviton mass and constraining Yukawa-like gravitational potential from planetary motion in the Solar System. in Filomat, 37(25), 8591-8601.
https://hdl.handle.net/21.15107/rcub_vinar_11358

Borka D, Borka Jovanović V, Jovanović P. Bounds on graviton mass and constraining Yukawa-like gravitational potential from planetary motion in the Solar System. in Filomat. 2023;37(25):8591-8601.
https://hdl.handle.net/21.15107/rcub_vinar_11358 .

Borka, Duško, Borka Jovanović, Vesna, Jovanović, Predrag, "Bounds on graviton mass and constraining Yukawa-like gravitational potential from planetary motion in the Solar System" in Filomat, 37, no. 25 (2023):8591-8601,
https://hdl.handle.net/21.15107/rcub_vinar_11358 .

TY  - JOUR
AU  - Stefanović, Bojana
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Dronjak, Slađana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8361
AB  - This study investigated the effects of melatonin treatment on adrenal catecholamine content, synthesis, uptake, and vesicular transport induced by the chronic unpredictable mild stress (CUMS) model of depression in rats. This entailed quantifying the norepinephrine, epinephrine, mRNA, and protein levels of tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), phenylethanolamine N-methyltransferase (PNMT), norepinephrine transporter (NET), and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. CUMS caused a significant depletion of norepinephrine stores and protein levels of TH, DBH, and NET, whereas the gene expression of PNMT was increased. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in norepinephrine content and the protein expression of TH, DBH, and NET in the adrenal medulla of chronically stressed rats. The present study demonstrates the stimulatory effect of melatonin on adrenomedullary synthesis, the uptake and content of catecholamine in the rat model of chronic stress-induced depression. © 2019, Published by NRC Research Press.
T2  - Canadian Journal of Physiology and Pharmacology
T1  - Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression
VL  - 97
IS  - 7
SP  - 685
EP  - 690
DO  - 10.1139/cjpp-2018-0612
ER  - 

@article{
author = "Stefanović, Bojana and Spasojević, Nataša and Jovanović, Predrag and Dronjak, Slađana",
year = "2019",
abstract = "This study investigated the effects of melatonin treatment on adrenal catecholamine content, synthesis, uptake, and vesicular transport induced by the chronic unpredictable mild stress (CUMS) model of depression in rats. This entailed quantifying the norepinephrine, epinephrine, mRNA, and protein levels of tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), phenylethanolamine N-methyltransferase (PNMT), norepinephrine transporter (NET), and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. CUMS caused a significant depletion of norepinephrine stores and protein levels of TH, DBH, and NET, whereas the gene expression of PNMT was increased. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in norepinephrine content and the protein expression of TH, DBH, and NET in the adrenal medulla of chronically stressed rats. The present study demonstrates the stimulatory effect of melatonin on adrenomedullary synthesis, the uptake and content of catecholamine in the rat model of chronic stress-induced depression. © 2019, Published by NRC Research Press.",
journal = "Canadian Journal of Physiology and Pharmacology",
title = "Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression",
volume = "97",
number = "7",
pages = "685-690",
doi = "10.1139/cjpp-2018-0612"
}

Stefanović, B., Spasojević, N., Jovanović, P.,& Dronjak, S.. (2019). Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression. in Canadian Journal of Physiology and Pharmacology, 97(7), 685-690.
https://doi.org/10.1139/cjpp-2018-0612

Stefanović B, Spasojević N, Jovanović P, Dronjak S. Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression. in Canadian Journal of Physiology and Pharmacology. 2019;97(7):685-690.
doi:10.1139/cjpp-2018-0612 .

Stefanović, Bojana, Spasojević, Nataša, Jovanović, Predrag, Dronjak, Slađana, "Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression" in Canadian Journal of Physiology and Pharmacology, 97, no. 7 (2019):685-690,
https://doi.org/10.1139/cjpp-2018-0612 . .

TY  - JOUR
AU  - Jovanović, Predrag
AU  - Spasojević, Nataša
AU  - Puškaš, Nela
AU  - Stefanović, Bojana
AU  - Dronjak, Slađana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8400
AB  - Social stress produces behavioral alterations, and autonomic and cardiac dysfunction in animals. In addition to the well-known roles of oxytocin on birth and maternal bonding, recent evidence shows that this neuropeptide possesses cardio-protective properties. However less is known about its role in the regulation of the autonomic nervous system. The direct influence of oxytocin on the cardiac catecholamine synthesizing enzyme, transport beta-adrenoceptors and muscarinic receptors in animals exposed to chronic social isolation stress has not yet been studied. In this study, we examined the influence of peripheral chronic oxytocin treatment on anxiety-related behavior, the morphology and content of epinephrine and norepinephrine, mRNA and protein levels of tyrosine hydroxylase (TH), norepinephrine transporter (NET) and receptors <beta> 3 (β3-AR) and muscarinic 2 (M2 MR) in the right and left cardiac atrium and ventricle of chronically socially isolated male rats. Our results show that oxytocin treatment exhibits an anxiolytic effect, decreases the heart/body weight ratio and prevents the hypertrophy of cardiomyocytes in the wall of the left ventricle of stressed rats. Epinephrine and TH protein levels were unchanged after prolonged oxytocin treatment. Peripheral oxytocin administration led to the enhancement of gene expression of β3-AR in both atria, NET protein in the left ventricle and gene expression of M2 MR in the right atrium and the left ventricle of chronically socially isolated rats. The study provides evidence that oxytocin treatment in chronically socially isolated animals enhances norepinephrine uptake and expression of cardio-inhibitory receptors in cardiac tissues, which could have a beneficial effect on the cardiovascular system under the increased activity of the sympathoneural system. © 2018 Elsevier Inc.
T2  - Peptides
T1  - Oxytocin modulates the expression of norepinephrine transporter, β3-adrenoceptors and muscarinic M2 receptors in the hearts of socially isolated rats
VL  - 111
SP  - 132
EP  - 141
DO  - 10.1016/j.peptides.2018.06.008
ER  - 

@article{
author = "Jovanović, Predrag and Spasojević, Nataša and Puškaš, Nela and Stefanović, Bojana and Dronjak, Slađana",
year = "2019",
abstract = "Social stress produces behavioral alterations, and autonomic and cardiac dysfunction in animals. In addition to the well-known roles of oxytocin on birth and maternal bonding, recent evidence shows that this neuropeptide possesses cardio-protective properties. However less is known about its role in the regulation of the autonomic nervous system. The direct influence of oxytocin on the cardiac catecholamine synthesizing enzyme, transport beta-adrenoceptors and muscarinic receptors in animals exposed to chronic social isolation stress has not yet been studied. In this study, we examined the influence of peripheral chronic oxytocin treatment on anxiety-related behavior, the morphology and content of epinephrine and norepinephrine, mRNA and protein levels of tyrosine hydroxylase (TH), norepinephrine transporter (NET) and receptors <beta> 3 (β3-AR) and muscarinic 2 (M2 MR) in the right and left cardiac atrium and ventricle of chronically socially isolated male rats. Our results show that oxytocin treatment exhibits an anxiolytic effect, decreases the heart/body weight ratio and prevents the hypertrophy of cardiomyocytes in the wall of the left ventricle of stressed rats. Epinephrine and TH protein levels were unchanged after prolonged oxytocin treatment. Peripheral oxytocin administration led to the enhancement of gene expression of β3-AR in both atria, NET protein in the left ventricle and gene expression of M2 MR in the right atrium and the left ventricle of chronically socially isolated rats. The study provides evidence that oxytocin treatment in chronically socially isolated animals enhances norepinephrine uptake and expression of cardio-inhibitory receptors in cardiac tissues, which could have a beneficial effect on the cardiovascular system under the increased activity of the sympathoneural system. © 2018 Elsevier Inc.",
journal = "Peptides",
title = "Oxytocin modulates the expression of norepinephrine transporter, β3-adrenoceptors and muscarinic M2 receptors in the hearts of socially isolated rats",
volume = "111",
pages = "132-141",
doi = "10.1016/j.peptides.2018.06.008"
}

Jovanović, P., Spasojević, N., Puškaš, N., Stefanović, B.,& Dronjak, S.. (2019). Oxytocin modulates the expression of norepinephrine transporter, β3-adrenoceptors and muscarinic M2 receptors in the hearts of socially isolated rats. in Peptides, 111, 132-141.
https://doi.org/10.1016/j.peptides.2018.06.008

Jovanović P, Spasojević N, Puškaš N, Stefanović B, Dronjak S. Oxytocin modulates the expression of norepinephrine transporter, β3-adrenoceptors and muscarinic M2 receptors in the hearts of socially isolated rats. in Peptides. 2019;111:132-141.
doi:10.1016/j.peptides.2018.06.008 .

Jovanović, Predrag, Spasojević, Nataša, Puškaš, Nela, Stefanović, Bojana, Dronjak, Slađana, "Oxytocin modulates the expression of norepinephrine transporter, β3-adrenoceptors and muscarinic M2 receptors in the hearts of socially isolated rats" in Peptides, 111 (2019):132-141,
https://doi.org/10.1016/j.peptides.2018.06.008 . .

TY  - JOUR
AU  - Stefanović, Bojana
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Dronjak, Slađana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8410
AB  - This study investigated the effects of melatonin treatment on adrenal catecholamine content, synthesis, uptake, and vesicular transport induced by the chronic unpredictable mild stress (CUMS) model of depression in rats. This entailed quantifying the norepinephrine, epinephrine, mRNA, and protein levels of tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), phenylethanolamine N-methyltransferase (PNMT), norepinephrine transporter (NET), and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. CUMS caused a significant depletion of norepinephrine stores and protein levels of TH, DBH, and NET, whereas the gene expression of PNMT was increased. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in norepinephrine content and the protein expression of TH, DBH, and NET in the adrenal medulla of chronically stressed rats. The present study demonstrates the stimulatory effect of melatonin on adrenomedullary synthesis, the uptake and content of catecholamine in the rat model of chronic stress-induced depression. © 2019, Published by NRC Research Press.
T2  - Canadian Journal of Physiology and Pharmacology
T1  - Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression
VL  - 97
IS  - 7
SP  - 685
EP  - 690
DO  - 10.1139/cjpp-2018-0612
ER  - 

@article{
author = "Stefanović, Bojana and Spasojević, Nataša and Jovanović, Predrag and Dronjak, Slađana",
year = "2019",
abstract = "This study investigated the effects of melatonin treatment on adrenal catecholamine content, synthesis, uptake, and vesicular transport induced by the chronic unpredictable mild stress (CUMS) model of depression in rats. This entailed quantifying the norepinephrine, epinephrine, mRNA, and protein levels of tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), phenylethanolamine N-methyltransferase (PNMT), norepinephrine transporter (NET), and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. CUMS caused a significant depletion of norepinephrine stores and protein levels of TH, DBH, and NET, whereas the gene expression of PNMT was increased. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in norepinephrine content and the protein expression of TH, DBH, and NET in the adrenal medulla of chronically stressed rats. The present study demonstrates the stimulatory effect of melatonin on adrenomedullary synthesis, the uptake and content of catecholamine in the rat model of chronic stress-induced depression. © 2019, Published by NRC Research Press.",
journal = "Canadian Journal of Physiology and Pharmacology",
title = "Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression",
volume = "97",
number = "7",
pages = "685-690",
doi = "10.1139/cjpp-2018-0612"
}

Stefanović, B., Spasojević, N., Jovanović, P.,& Dronjak, S.. (2019). Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression. in Canadian Journal of Physiology and Pharmacology, 97(7), 685-690.
https://doi.org/10.1139/cjpp-2018-0612

Stefanović B, Spasojević N, Jovanović P, Dronjak S. Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression. in Canadian Journal of Physiology and Pharmacology. 2019;97(7):685-690.
doi:10.1139/cjpp-2018-0612 .

Stefanović, Bojana, Spasojević, Nataša, Jovanović, Predrag, Dronjak, Slađana, "Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression" in Canadian Journal of Physiology and Pharmacology, 97, no. 7 (2019):685-690,
https://doi.org/10.1139/cjpp-2018-0612 . .

TY  - JOUR
AU  - Stefanović, Bojana
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Ferizović, Harisa
AU  - Dronjak, Slađana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7795
AB  - OBJECTIVE: This study investigated the effects of chronic melatonin treatment on gene expression of α1-, α2-, β1- and β2-adrenoceptors in the hippocampus of rats subjected to chronic unpredictable mild stress (CUMS). BACKGROUND: Preclinical studies have also shown that melatonin prevented short- and long-term memory impairments and exhibited antidepressant-like actions. METHODS: For this study, we used 24 animals, which were divided into four groups, and the experiment lasted 4 weeks. We quantifi ed the changes in mRNA and protein levels of α1-, α2-, β1- and β2-adrenoceptors in the hippocampus after melatonin treatment. RESULTS: Our results demonstrated a decreased gene expression of α1-, α2- and β2-adrenoceptors in the hippocampus of rats subjected to unpredictable chronic mild stress, while there was no change in gene expression of β1-adrenoceptors. Melatonin treatment in the CUMS rats prevented the stress-induced decrease in mRNA and protein levels of α1-and β2-adrenoceptors, whereas did not affect either on mRNA or protein level of β1-and α2-adrenoceptors. CONCLUSION: Our data suggest that melatonin, by increasing reduced levels of α1- and β2-adrenoceptors mRNA and protein in the hippocampus of chronic stressed rats, may be benefi cial in conditions such as chronic stress and provides an experimental opportunity to probe into further molecular mechanisms underlying the regulation of these receptor subtype.
T2  - Bratislava Medical Journal
T1  - Melatonin modulate the expression of α1- and β2-adrenoceptors in the hippocampus of rats subjected to unpredictable chronic mild stress
VL  - 119
IS  - 7
SP  - 429
EP  - 433
DO  - 10.4149/BLL_2018_078
ER  - 

@article{
author = "Stefanović, Bojana and Spasojević, Nataša and Jovanović, Predrag and Ferizović, Harisa and Dronjak, Slađana",
year = "2018",
abstract = "OBJECTIVE: This study investigated the effects of chronic melatonin treatment on gene expression of α1-, α2-, β1- and β2-adrenoceptors in the hippocampus of rats subjected to chronic unpredictable mild stress (CUMS). BACKGROUND: Preclinical studies have also shown that melatonin prevented short- and long-term memory impairments and exhibited antidepressant-like actions. METHODS: For this study, we used 24 animals, which were divided into four groups, and the experiment lasted 4 weeks. We quantifi ed the changes in mRNA and protein levels of α1-, α2-, β1- and β2-adrenoceptors in the hippocampus after melatonin treatment. RESULTS: Our results demonstrated a decreased gene expression of α1-, α2- and β2-adrenoceptors in the hippocampus of rats subjected to unpredictable chronic mild stress, while there was no change in gene expression of β1-adrenoceptors. Melatonin treatment in the CUMS rats prevented the stress-induced decrease in mRNA and protein levels of α1-and β2-adrenoceptors, whereas did not affect either on mRNA or protein level of β1-and α2-adrenoceptors. CONCLUSION: Our data suggest that melatonin, by increasing reduced levels of α1- and β2-adrenoceptors mRNA and protein in the hippocampus of chronic stressed rats, may be benefi cial in conditions such as chronic stress and provides an experimental opportunity to probe into further molecular mechanisms underlying the regulation of these receptor subtype.",
journal = "Bratislava Medical Journal",
title = "Melatonin modulate the expression of α1- and β2-adrenoceptors in the hippocampus of rats subjected to unpredictable chronic mild stress",
volume = "119",
number = "7",
pages = "429-433",
doi = "10.4149/BLL_2018_078"
}

Stefanović, B., Spasojević, N., Jovanović, P., Ferizović, H.,& Dronjak, S.. (2018). Melatonin modulate the expression of α1- and β2-adrenoceptors in the hippocampus of rats subjected to unpredictable chronic mild stress. in Bratislava Medical Journal, 119(7), 429-433.
https://doi.org/10.4149/BLL_2018_078

Stefanović B, Spasojević N, Jovanović P, Ferizović H, Dronjak S. Melatonin modulate the expression of α1- and β2-adrenoceptors in the hippocampus of rats subjected to unpredictable chronic mild stress. in Bratislava Medical Journal. 2018;119(7):429-433.
doi:10.4149/BLL_2018_078 .

Stefanović, Bojana, Spasojević, Nataša, Jovanović, Predrag, Ferizović, Harisa, Dronjak, Slađana, "Melatonin modulate the expression of α1- and β2-adrenoceptors in the hippocampus of rats subjected to unpredictable chronic mild stress" in Bratislava Medical Journal, 119, no. 7 (2018):429-433,
https://doi.org/10.4149/BLL_2018_078 . .

TY  - CONF
AU  - Stefanović, Bojana
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Dronjak, Slađana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12289
AB  - Introduction: Stress is considered a determinant in the etiology of depression. The adrenal medulla plays a key role in response to stress by releasing catecholamines, which are important to maintain homeostasis. Many studies have assessed the antidepressant-like activity of the melatonin, a neurohormone synthesized in the pineal gland. Thus, in the present study, we examined the effect of chronic melatonin treatment on mRNA levels and protein content of catecholamines biosynthetic enzymes (TH, DBH and PNMT) and transporters (NET, VMAT2) in adrenal medulla of rats exposed to chronic unpredictable mild stress (CUMS). Material and Methods: CUMS was used as an animal model of depression. Exposure of rats to CUMS and placebo or melatonin (10 mg/kg body weight, i.p.) administration started on the same day and was continued for 4 weeks. For quantifying TH, DBH, PNMT, NET and VMAT2 mRNA and protein levels we used realtime PCR and Western blot analysis. Results: We observed that CUMS induced increased mRNA levels of catecholamine biosynthetic enzymes (TH, DBH, and PNMT), and noradrenaline transporter NET, while treatment with melatonin decreased these biosynthetic enzymes and transporter. Conversely, CUMS induced a decrease in protein content of TH and NET, while chronic melatonin treatment increased NET protein levels in both control and stressed rats. CUMS and melatonin treatment has no effect on mRNA levels and protein content of VMAT2. Conclusion: This study suggests that the observed decrease of catecholamine biosynthesis and enhanced re-uptake in adrenal medulla of rats exposed to CUMS are connected to the beneficial effects of chronic melatonin treatment.
PB  - Belgrade : University of Belgrade, Faculty of Biology
C3  - CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
T1  - Melatonin modulates catecholamine biosynthesis and re-uptake in adrenal medulla of rats exposed to chronic unpredictable mild stress
SP  - 77
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12289
ER  - 

@conference{
author = "Stefanović, Bojana and Spasojević, Nataša and Jovanović, Predrag and Dronjak, Slađana",
year = "2017",
abstract = "Introduction: Stress is considered a determinant in the etiology of depression. The adrenal medulla plays a key role in response to stress by releasing catecholamines, which are important to maintain homeostasis. Many studies have assessed the antidepressant-like activity of the melatonin, a neurohormone synthesized in the pineal gland. Thus, in the present study, we examined the effect of chronic melatonin treatment on mRNA levels and protein content of catecholamines biosynthetic enzymes (TH, DBH and PNMT) and transporters (NET, VMAT2) in adrenal medulla of rats exposed to chronic unpredictable mild stress (CUMS). Material and Methods: CUMS was used as an animal model of depression. Exposure of rats to CUMS and placebo or melatonin (10 mg/kg body weight, i.p.) administration started on the same day and was continued for 4 weeks. For quantifying TH, DBH, PNMT, NET and VMAT2 mRNA and protein levels we used realtime PCR and Western blot analysis. Results: We observed that CUMS induced increased mRNA levels of catecholamine biosynthetic enzymes (TH, DBH, and PNMT), and noradrenaline transporter NET, while treatment with melatonin decreased these biosynthetic enzymes and transporter. Conversely, CUMS induced a decrease in protein content of TH and NET, while chronic melatonin treatment increased NET protein levels in both control and stressed rats. CUMS and melatonin treatment has no effect on mRNA levels and protein content of VMAT2. Conclusion: This study suggests that the observed decrease of catecholamine biosynthesis and enhanced re-uptake in adrenal medulla of rats exposed to CUMS are connected to the beneficial effects of chronic melatonin treatment.",
publisher = "Belgrade : University of Belgrade, Faculty of Biology",
journal = "CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts",
title = "Melatonin modulates catecholamine biosynthesis and re-uptake in adrenal medulla of rats exposed to chronic unpredictable mild stress",
pages = "77-77",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12289"
}

Stefanović, B., Spasojević, N., Jovanović, P.,& Dronjak, S.. (2017). Melatonin modulates catecholamine biosynthesis and re-uptake in adrenal medulla of rats exposed to chronic unpredictable mild stress. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
Belgrade : University of Belgrade, Faculty of Biology., 77-77.
https://hdl.handle.net/21.15107/rcub_vinar_12289

Stefanović B, Spasojević N, Jovanović P, Dronjak S. Melatonin modulates catecholamine biosynthesis and re-uptake in adrenal medulla of rats exposed to chronic unpredictable mild stress. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts. 2017;:77-77.
https://hdl.handle.net/21.15107/rcub_vinar_12289 .

Stefanović, Bojana, Spasojević, Nataša, Jovanović, Predrag, Dronjak, Slađana, "Melatonin modulates catecholamine biosynthesis and re-uptake in adrenal medulla of rats exposed to chronic unpredictable mild stress" in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts (2017):77-77,
https://hdl.handle.net/21.15107/rcub_vinar_12289 .

TY  - JOUR
AU  - Dronjak, Slađana
AU  - Stefanović, Bojana
AU  - Jovanović, Predrag
AU  - Spasojević, Nataša
AU  - Janković, Milica
AU  - Jeremić, Ivica
AU  - Hoffmann, Markus
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1929
AB  - Baseline sympathetic activity was found to be elevated in rheumatoid arthritis (RA) patients and it is related to increased cardiovascular risk in these patients. Although many studies have highlighted the association between RA and increased cardiac sympathetic activity, the underlying mechanistic links remain unclear. The aim of the present study was to understand how diseases-triggered changes in gene expression may result in maladaptive physiological changes. Our results suggest that the equilibrium between noradrenaline synthesis, release and reuptake was disrupted in the ventricles of arthritic rats. In the acute phase of the arthritic process, decreased gene expression of MAO-A might lead to accumulation of noradrenaline in myocardial interstitial space, whereas increased gene expression of NET protected cardiomyocytes from the deleterious effects of enhanced noradrenaline. During the chronic phase, reduced expression of beta(1)-adrenoceptor and decreased efficiency of noradrenaline reuptake contribute to progressive damage of the myocardium and limits heart efficiency.
T2  - Autonomic Neuroscience: Basic and Clinical
T1  - Altered cardiac gene expression of noradrenaline enzymes, transporter and beta-adrenoceptors in rat model of rheumatoid arthritis
VL  - 208
SP  - 165
EP  - 169
DO  - 10.1016/j.autneu.2017.10.003
ER  - 

@article{
author = "Dronjak, Slađana and Stefanović, Bojana and Jovanović, Predrag and Spasojević, Nataša and Janković, Milica and Jeremić, Ivica and Hoffmann, Markus",
year = "2017",
abstract = "Baseline sympathetic activity was found to be elevated in rheumatoid arthritis (RA) patients and it is related to increased cardiovascular risk in these patients. Although many studies have highlighted the association between RA and increased cardiac sympathetic activity, the underlying mechanistic links remain unclear. The aim of the present study was to understand how diseases-triggered changes in gene expression may result in maladaptive physiological changes. Our results suggest that the equilibrium between noradrenaline synthesis, release and reuptake was disrupted in the ventricles of arthritic rats. In the acute phase of the arthritic process, decreased gene expression of MAO-A might lead to accumulation of noradrenaline in myocardial interstitial space, whereas increased gene expression of NET protected cardiomyocytes from the deleterious effects of enhanced noradrenaline. During the chronic phase, reduced expression of beta(1)-adrenoceptor and decreased efficiency of noradrenaline reuptake contribute to progressive damage of the myocardium and limits heart efficiency.",
journal = "Autonomic Neuroscience: Basic and Clinical",
title = "Altered cardiac gene expression of noradrenaline enzymes, transporter and beta-adrenoceptors in rat model of rheumatoid arthritis",
volume = "208",
pages = "165-169",
doi = "10.1016/j.autneu.2017.10.003"
}

Dronjak, S., Stefanović, B., Jovanović, P., Spasojević, N., Janković, M., Jeremić, I.,& Hoffmann, M.. (2017). Altered cardiac gene expression of noradrenaline enzymes, transporter and beta-adrenoceptors in rat model of rheumatoid arthritis. in Autonomic Neuroscience: Basic and Clinical, 208, 165-169.
https://doi.org/10.1016/j.autneu.2017.10.003

Dronjak S, Stefanović B, Jovanović P, Spasojević N, Janković M, Jeremić I, Hoffmann M. Altered cardiac gene expression of noradrenaline enzymes, transporter and beta-adrenoceptors in rat model of rheumatoid arthritis. in Autonomic Neuroscience: Basic and Clinical. 2017;208:165-169.
doi:10.1016/j.autneu.2017.10.003 .

Dronjak, Slađana, Stefanović, Bojana, Jovanović, Predrag, Spasojević, Nataša, Janković, Milica, Jeremić, Ivica, Hoffmann, Markus, "Altered cardiac gene expression of noradrenaline enzymes, transporter and beta-adrenoceptors in rat model of rheumatoid arthritis" in Autonomic Neuroscience: Basic and Clinical, 208 (2017):165-169,
https://doi.org/10.1016/j.autneu.2017.10.003 . .

TY  - JOUR
AU  - Borka, Duško
AU  - Borka Jovanović, Vesna
AU  - Jovanović, Predrag
AU  - Capozziello, Salvatore
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11897
AB  - We investigate the possibility to explain theoretically the S2 star orbital precession around the massive object at Galactic Centre using Extended Theories of Gravity, specifically f(R, φ) a Sanders-like gravitational potential in total absence of dark matter. To this aim an analytic fourth-order theory of gravity, non-minimally coupled with a massive scalar field is considered. The interaction term is given by an analytic functions f(R, φ) where R is the Ricci scalar and φ is the scalar field. We simulated orbit of S2 star around Galactic Centre in Sanders-like gravity potentials and compared it with NTT/VLT observations. We presented maps of reduced χ 2 over the {α − mφ} parameter space in the case of NTT/VLT observations. The approach we are proposing seems reliable to constrain modified gravity models at astronomical level.
T2  - Publications of the Astronomical Observatory of Belgrade
T1  - Constraints on f(R, ɸ) (Sanders-Like) Gravity Potential From Orbit of S2 Star
IS  - 96
SP  - 205
EP  - 210
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11897
ER  - 

@article{
author = "Borka, Duško and Borka Jovanović, Vesna and Jovanović, Predrag and Capozziello, Salvatore",
year = "2017",
abstract = "We investigate the possibility to explain theoretically the S2 star orbital precession around the massive object at Galactic Centre using Extended Theories of Gravity, specifically f(R, φ) a Sanders-like gravitational potential in total absence of dark matter. To this aim an analytic fourth-order theory of gravity, non-minimally coupled with a massive scalar field is considered. The interaction term is given by an analytic functions f(R, φ) where R is the Ricci scalar and φ is the scalar field. We simulated orbit of S2 star around Galactic Centre in Sanders-like gravity potentials and compared it with NTT/VLT observations. We presented maps of reduced χ 2 over the {α − mφ} parameter space in the case of NTT/VLT observations. The approach we are proposing seems reliable to constrain modified gravity models at astronomical level.",
journal = "Publications of the Astronomical Observatory of Belgrade",
title = "Constraints on f(R, ɸ) (Sanders-Like) Gravity Potential From Orbit of S2 Star",
number = "96",
pages = "205-210",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11897"
}

Borka, D., Borka Jovanović, V., Jovanović, P.,& Capozziello, S.. (2017). Constraints on f(R, ɸ) (Sanders-Like) Gravity Potential From Orbit of S2 Star. in Publications of the Astronomical Observatory of Belgrade(96), 205-210.
https://hdl.handle.net/21.15107/rcub_vinar_11897

Borka D, Borka Jovanović V, Jovanović P, Capozziello S. Constraints on f(R, ɸ) (Sanders-Like) Gravity Potential From Orbit of S2 Star. in Publications of the Astronomical Observatory of Belgrade. 2017;(96):205-210.
https://hdl.handle.net/21.15107/rcub_vinar_11897 .

Borka, Duško, Borka Jovanović, Vesna, Jovanović, Predrag, Capozziello, Salvatore, "Constraints on f(R, ɸ) (Sanders-Like) Gravity Potential From Orbit of S2 Star" in Publications of the Astronomical Observatory of Belgrade, no. 96 (2017):205-210,
https://hdl.handle.net/21.15107/rcub_vinar_11897 .

TY  - JOUR
AU  - Stanić, Dušanka
AU  - Plećaš-Solarović, Bosiljka
AU  - Mirković, Duško
AU  - Jovanović, Predrag
AU  - Dronjak, Slađana
AU  - Marković, Bojan D.
AU  - Đorđević, Tea
AU  - Ignjatović, Svetlana
AU  - Pešić, Vesna
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1595
AB  - Chronic stress conditions can lead to considerable and extensible changes in physiological and psychological performances, and in emergence of risk for various somatic diseases. On the other hand, the neuropeptide oxytocin is reported to increase the resistance of the organism to stress and modulate activity of autonomic nervous system. Chronic corticosterone administration is used as a rat model for a state observed in terms of chronic stress exposure, when negative feedback mechanism of hypothalamus-pituitary-adrenal axis activity is disrupted. In our study, we aimed to investigate whether chronic administration of oxytocin (10114400 pI/day for 14 days, s.c.) influenced adrenal gland morphology and activity in adult male Wistar rats during long-term corticosterone administration via drinking water (100 mg/L for 21 days). We examined the influence of treatments on the levels of adrenal gland hormones, corticosterone, adrenaline and noradrenaline, as well as their response to an acute stress challenge evoked by 15-min forced swimming. In addition, the expression of two main monoamine transporters, the noradrenaline transporter (NAT) and vesicular monoamine transporter 2 (VMAT2) in adrenal medulla was measured in the rats exposed to acute stress. Our results showed that oxytocin treatment prevented corticosterone-induced decrease in body weight gain, attenuated adrenal gland atrophy by increasing glandular weight, and the area of the zona fasciculate and reticularis. Chronic corticosterone intake blunted the response of all measured hormones to acute stress, whereas concomitant oxytocin treatment reversed adrenaline and noradrenaline response to acute stress. Furthermore, in adrenal medulla, oxytocin produced significant vasodilatation and stimulated expression of both catecholamine transporters detected both on mRNA and protein level. Our data suggest that oxytocin, by reducing atrophy of adrenal gland, and by increasing catecholamine storage capacity, may be beneficial in conditions accompanied with high glucocorticoid levels, such as chronic stress exposure. (C) 2017 Elsevier Ltd. All rights reserved.
T2  - Psychoneuroendocrinology
T1  - Oxytocin in corticosterone-induced chronic stress model: Focus on adrenal gland function
VL  - 80
SP  - 137
EP  - 146
DO  - 10.1016/j.psyneuen.2017.03.011
ER  - 

@article{
author = "Stanić, Dušanka and Plećaš-Solarović, Bosiljka and Mirković, Duško and Jovanović, Predrag and Dronjak, Slađana and Marković, Bojan D. and Đorđević, Tea and Ignjatović, Svetlana and Pešić, Vesna",
year = "2017",
abstract = "Chronic stress conditions can lead to considerable and extensible changes in physiological and psychological performances, and in emergence of risk for various somatic diseases. On the other hand, the neuropeptide oxytocin is reported to increase the resistance of the organism to stress and modulate activity of autonomic nervous system. Chronic corticosterone administration is used as a rat model for a state observed in terms of chronic stress exposure, when negative feedback mechanism of hypothalamus-pituitary-adrenal axis activity is disrupted. In our study, we aimed to investigate whether chronic administration of oxytocin (10114400 pI/day for 14 days, s.c.) influenced adrenal gland morphology and activity in adult male Wistar rats during long-term corticosterone administration via drinking water (100 mg/L for 21 days). We examined the influence of treatments on the levels of adrenal gland hormones, corticosterone, adrenaline and noradrenaline, as well as their response to an acute stress challenge evoked by 15-min forced swimming. In addition, the expression of two main monoamine transporters, the noradrenaline transporter (NAT) and vesicular monoamine transporter 2 (VMAT2) in adrenal medulla was measured in the rats exposed to acute stress. Our results showed that oxytocin treatment prevented corticosterone-induced decrease in body weight gain, attenuated adrenal gland atrophy by increasing glandular weight, and the area of the zona fasciculate and reticularis. Chronic corticosterone intake blunted the response of all measured hormones to acute stress, whereas concomitant oxytocin treatment reversed adrenaline and noradrenaline response to acute stress. Furthermore, in adrenal medulla, oxytocin produced significant vasodilatation and stimulated expression of both catecholamine transporters detected both on mRNA and protein level. Our data suggest that oxytocin, by reducing atrophy of adrenal gland, and by increasing catecholamine storage capacity, may be beneficial in conditions accompanied with high glucocorticoid levels, such as chronic stress exposure. (C) 2017 Elsevier Ltd. All rights reserved.",
journal = "Psychoneuroendocrinology",
title = "Oxytocin in corticosterone-induced chronic stress model: Focus on adrenal gland function",
volume = "80",
pages = "137-146",
doi = "10.1016/j.psyneuen.2017.03.011"
}

Stanić, D., Plećaš-Solarović, B., Mirković, D., Jovanović, P., Dronjak, S., Marković, B. D., Đorđević, T., Ignjatović, S.,& Pešić, V.. (2017). Oxytocin in corticosterone-induced chronic stress model: Focus on adrenal gland function. in Psychoneuroendocrinology, 80, 137-146.
https://doi.org/10.1016/j.psyneuen.2017.03.011

Stanić D, Plećaš-Solarović B, Mirković D, Jovanović P, Dronjak S, Marković BD, Đorđević T, Ignjatović S, Pešić V. Oxytocin in corticosterone-induced chronic stress model: Focus on adrenal gland function. in Psychoneuroendocrinology. 2017;80:137-146.
doi:10.1016/j.psyneuen.2017.03.011 .

Stanić, Dušanka, Plećaš-Solarović, Bosiljka, Mirković, Duško, Jovanović, Predrag, Dronjak, Slađana, Marković, Bojan D., Đorđević, Tea, Ignjatović, Svetlana, Pešić, Vesna, "Oxytocin in corticosterone-induced chronic stress model: Focus on adrenal gland function" in Psychoneuroendocrinology, 80 (2017):137-146,
https://doi.org/10.1016/j.psyneuen.2017.03.011 . .

TY  - CONF
AU  - Stanic, D.
AU  - Petrovic, J.
AU  - Mirkovic, D.
AU  - Dordevic, T.
AU  - Duric, V.
AU  - Jovanović, Predrag
AU  - Dronjak, Slađana
AU  - Pesic, V.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7159
C3  - European Neuropsychopharmacology
T1  - Oxytocin modulates HPA axis activity and hormone response to stress in rats chronically treated with corticosterone
VL  - 26
SP  - S204
EP  - S204
DO  - 10.1016/S0924-977X(16)31048-3
ER  - 

@conference{
author = "Stanic, D. and Petrovic, J. and Mirkovic, D. and Dordevic, T. and Duric, V. and Jovanović, Predrag and Dronjak, Slađana and Pesic, V.",
year = "2016",
journal = "European Neuropsychopharmacology",
title = "Oxytocin modulates HPA axis activity and hormone response to stress in rats chronically treated with corticosterone",
volume = "26",
pages = "S204-S204",
doi = "10.1016/S0924-977X(16)31048-3"
}

Stanic, D., Petrovic, J., Mirkovic, D., Dordevic, T., Duric, V., Jovanović, P., Dronjak, S.,& Pesic, V.. (2016). Oxytocin modulates HPA axis activity and hormone response to stress in rats chronically treated with corticosterone. in European Neuropsychopharmacology, 26, S204-S204.
https://doi.org/10.1016/S0924-977X(16)31048-3

Stanic D, Petrovic J, Mirkovic D, Dordevic T, Duric V, Jovanović P, Dronjak S, Pesic V. Oxytocin modulates HPA axis activity and hormone response to stress in rats chronically treated with corticosterone. in European Neuropsychopharmacology. 2016;26:S204-S204.
doi:10.1016/S0924-977X(16)31048-3 .

Stanic, D., Petrovic, J., Mirkovic, D., Dordevic, T., Duric, V., Jovanović, Predrag, Dronjak, Slađana, Pesic, V., "Oxytocin modulates HPA axis activity and hormone response to stress in rats chronically treated with corticosterone" in European Neuropsychopharmacology, 26 (2016):S204-S204,
https://doi.org/10.1016/S0924-977X(16)31048-3 . .

TY  - JOUR
AU  - Jovanović, Predrag
AU  - Stefanović, Bojana
AU  - Spasojević, Nataša
AU  - Puškaš, Nela
AU  - Dronjak, Slađana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1074
AB  - Purpose/aim: The adreno-medullar system represents one of the main systems involved in the response to stressful events. The neuropeptide oxytocin, is highly sensitive to the social environment, and regulates autonomic function. Adreno-medullary activity is dependent on the synthesis of catecholamine, its reuptake, release, degradation and vesicular transport. A direct influence of oxytocin on catecholamine synthesizing enzyme and transports in animals exposed to chronic social isolation stress has not been studied yet. Materials and methods: In the present study, we examined the effect of chronic oxytocin treatment on the level of plasma catecholamine and its content, mRNA and protein levels of tyrosine hydroxylase (TH), noradrenaline transporter (NET) as well as vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla of socially isolated rats. Results: Our results show that, by the end of 12 weeks, social isolation did not produce any significant changes in catecholamine content but increased plasma catecholamine level and synthesis in the adrenal medulla. Oxytocin treatment had no further effect either on catecholamine synthesis or content in socially stressed animals whereas a significant elevation of plasma norepinephrine and epinephrine were reduced. On the other hand, chronic isolation caused a significant increase in VMAT2 and decrease in NET protein levels. Oxytocin treatment brought about an increase in protein levels of NET and its return to the levels of control group. Besides, it further increases VMAT2 protein levels in the adrenal medulla of individually housed rats. Conclusion: The present results show that peripheral oxytocin treatment enhances catecholamine uptake and storage in the adrenal medulla of chronically isolated animals.
PB  - Taylor & Francis
T2  - Endocrine Research
T1  - Effects of oxytocin on adreno-medullary catecholamine synthesis, uptake and storage in rats exposed to chronic isolation stress
VL  - 41
IS  - 2
SP  - 124
EP  - 131
DO  - 10.3109/07435800.2015.1094086
ER  - 

@article{
author = "Jovanović, Predrag and Stefanović, Bojana and Spasojević, Nataša and Puškaš, Nela and Dronjak, Slađana",
year = "2016",
abstract = "Purpose/aim: The adreno-medullar system represents one of the main systems involved in the response to stressful events. The neuropeptide oxytocin, is highly sensitive to the social environment, and regulates autonomic function. Adreno-medullary activity is dependent on the synthesis of catecholamine, its reuptake, release, degradation and vesicular transport. A direct influence of oxytocin on catecholamine synthesizing enzyme and transports in animals exposed to chronic social isolation stress has not been studied yet. Materials and methods: In the present study, we examined the effect of chronic oxytocin treatment on the level of plasma catecholamine and its content, mRNA and protein levels of tyrosine hydroxylase (TH), noradrenaline transporter (NET) as well as vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla of socially isolated rats. Results: Our results show that, by the end of 12 weeks, social isolation did not produce any significant changes in catecholamine content but increased plasma catecholamine level and synthesis in the adrenal medulla. Oxytocin treatment had no further effect either on catecholamine synthesis or content in socially stressed animals whereas a significant elevation of plasma norepinephrine and epinephrine were reduced. On the other hand, chronic isolation caused a significant increase in VMAT2 and decrease in NET protein levels. Oxytocin treatment brought about an increase in protein levels of NET and its return to the levels of control group. Besides, it further increases VMAT2 protein levels in the adrenal medulla of individually housed rats. Conclusion: The present results show that peripheral oxytocin treatment enhances catecholamine uptake and storage in the adrenal medulla of chronically isolated animals.",
publisher = "Taylor & Francis",
journal = "Endocrine Research",
title = "Effects of oxytocin on adreno-medullary catecholamine synthesis, uptake and storage in rats exposed to chronic isolation stress",
volume = "41",
number = "2",
pages = "124-131",
doi = "10.3109/07435800.2015.1094086"
}

Jovanović, P., Stefanović, B., Spasojević, N., Puškaš, N.,& Dronjak, S.. (2016). Effects of oxytocin on adreno-medullary catecholamine synthesis, uptake and storage in rats exposed to chronic isolation stress. in Endocrine Research
Taylor & Francis., 41(2), 124-131.
https://doi.org/10.3109/07435800.2015.1094086

Jovanović P, Stefanović B, Spasojević N, Puškaš N, Dronjak S. Effects of oxytocin on adreno-medullary catecholamine synthesis, uptake and storage in rats exposed to chronic isolation stress. in Endocrine Research. 2016;41(2):124-131.
doi:10.3109/07435800.2015.1094086 .

Jovanović, Predrag, Stefanović, Bojana, Spasojević, Nataša, Puškaš, Nela, Dronjak, Slađana, "Effects of oxytocin on adreno-medullary catecholamine synthesis, uptake and storage in rats exposed to chronic isolation stress" in Endocrine Research, 41, no. 2 (2016):124-131,
https://doi.org/10.3109/07435800.2015.1094086 . .

TY  - JOUR
AU  - Stefanović, Bojana
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Jasnić, Nebojša
AU  - Đorđević, Jelena D.
AU  - Dronjak, Slađana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1265
AB  - The hippocampus is sensitive to stress which activates norepinephrine terminals deriving from the locus coeruleus. Melatonin exerts positive effects on the hippocampal neurogenic process and on depressive-like behaviour. Thus, in the present study, an examination was made of the effect of chronic melatonin treatment on norepinephrine content, synthesis, uptake, vesicular transport and degradation in the hippocampus of rats exposed to CUMS. This entailed quantifying the norephinephrine, mRNA and protein levels of DBH, NET, VMAT 2, MAO-A and COMT. The results show that CUMS evoked prolonged immobility. Melatonin treatment decreased immobility in comparison with the placebo group, reflecting an antidepressant-like effect. Compared with the placebo group, a dramatic decrease in norepinephrine content, decreased VMAT2 mRNA and protein and increased MAO-A protein levels in the hippocampus of the CUMS rats were observed. However, no significant differences in the levels of DBH, NET, COMT mRNA and protein and MAO-A mRNA levels between the placebo and the stressed groups were found. The results showed the restorative effects of melatonin on the stress-induced decline in the norepinephrine content of the hippocampus. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in VMAT2 mRNA and protein levels, whereas it reduced the increase of the mRNA of COMT and protein levels of MAO-A. Chronic treatment with melatonin failed to alter the gene expression of DBH or NET in the hippocampus of the CUMS rats. Additionally, the results show that melatonin enhances VMAT2 expression and norepinephrine storage, whilst it reduces norepinephrine degrading enzymes. (C) 2016 Elsevier B.V. and ECNP. All rights reserved.
T2  - European Neuropsychopharmacology
T1  - Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels
VL  - 26
IS  - 10
SP  - 1629
EP  - 1637
DO  - 10.1016/j.euroneuro.2016.07.005
ER  - 

@article{
author = "Stefanović, Bojana and Spasojević, Nataša and Jovanović, Predrag and Jasnić, Nebojša and Đorđević, Jelena D. and Dronjak, Slađana",
year = "2016",
abstract = "The hippocampus is sensitive to stress which activates norepinephrine terminals deriving from the locus coeruleus. Melatonin exerts positive effects on the hippocampal neurogenic process and on depressive-like behaviour. Thus, in the present study, an examination was made of the effect of chronic melatonin treatment on norepinephrine content, synthesis, uptake, vesicular transport and degradation in the hippocampus of rats exposed to CUMS. This entailed quantifying the norephinephrine, mRNA and protein levels of DBH, NET, VMAT 2, MAO-A and COMT. The results show that CUMS evoked prolonged immobility. Melatonin treatment decreased immobility in comparison with the placebo group, reflecting an antidepressant-like effect. Compared with the placebo group, a dramatic decrease in norepinephrine content, decreased VMAT2 mRNA and protein and increased MAO-A protein levels in the hippocampus of the CUMS rats were observed. However, no significant differences in the levels of DBH, NET, COMT mRNA and protein and MAO-A mRNA levels between the placebo and the stressed groups were found. The results showed the restorative effects of melatonin on the stress-induced decline in the norepinephrine content of the hippocampus. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in VMAT2 mRNA and protein levels, whereas it reduced the increase of the mRNA of COMT and protein levels of MAO-A. Chronic treatment with melatonin failed to alter the gene expression of DBH or NET in the hippocampus of the CUMS rats. Additionally, the results show that melatonin enhances VMAT2 expression and norepinephrine storage, whilst it reduces norepinephrine degrading enzymes. (C) 2016 Elsevier B.V. and ECNP. All rights reserved.",
journal = "European Neuropsychopharmacology",
title = "Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels",
volume = "26",
number = "10",
pages = "1629-1637",
doi = "10.1016/j.euroneuro.2016.07.005"
}

Stefanović, B., Spasojević, N., Jovanović, P., Jasnić, N., Đorđević, J. D.,& Dronjak, S.. (2016). Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels. in European Neuropsychopharmacology, 26(10), 1629-1637.
https://doi.org/10.1016/j.euroneuro.2016.07.005

Stefanović B, Spasojević N, Jovanović P, Jasnić N, Đorđević JD, Dronjak S. Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels. in European Neuropsychopharmacology. 2016;26(10):1629-1637.
doi:10.1016/j.euroneuro.2016.07.005 .

Stefanović, Bojana, Spasojević, Nataša, Jovanović, Predrag, Jasnić, Nebojša, Đorđević, Jelena D., Dronjak, Slađana, "Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels" in European Neuropsychopharmacology, 26, no. 10 (2016):1629-1637,
https://doi.org/10.1016/j.euroneuro.2016.07.005 . .

TY  - JOUR
AU  - Spasojević, Nataša
AU  - Stefanović, Bojana
AU  - Jovanović, Predrag
AU  - Dronjak, Slađana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1495
AB  - Preclinical studies have shown that melatonin exercised antidepressant-like and anxiolytic-like effects in animal models of anxiety. The aim of the present study was to correlate the changes in behaviour induced by melatonin treatment with the activity of the dopaminergic system in the hippocampus of Wistar rats exposed to chronic, unpredictable, mild stress (CUMS). Male Wistar rats, 11 weeks old, were subjected to chronic stress for 28 successive days. Separate groups of control and stressed rats were intraperitoneally injected daily either with melatonin (10 mg/kg/day, i.p.) or placebo (5% ethanol). The open-field and elevated plus-maze tests were used to assess locomotor activities and anxiety levels. The content of dopamine (DA) in the hippocampal tissues was determined using radioenzymatic assay, while changes in tyrosine hydroxylase (TH) mRNA and protein levels in the hippocampus were determined using real-time RT-PCR and Western immunoblotting. Chronic stress led to reduction in the hippocampal dopaminergic content without affecting the levels of TH protein. These changes were accompanied by increased locomotor activity and higher anxiety levels in the open-field test. Administration of melatonin for 28 days resulted in an increase in the hippocampal DA content as a result of elevated TH protein levels. Melatonin showed an improvement in anxiety-like behaviour along with significantly reduced exploration. We could conclude that melatonin may stimulate dopaminergic synthesis in the hippocampus in order to suppress stress-induced behaviour.
T2  - Folia Biologica
T1  - Anxiety and Hyperlocomotion Induced by Chronic Unpredictable Mild Stress Can Be Moderated with Melatonin Treatment
VL  - 62
IS  - 6
SP  - 250
EP  - 257
UR  - https://hdl.handle.net/21.15107/rcub_vinar_1495
ER  - 

@article{
author = "Spasojević, Nataša and Stefanović, Bojana and Jovanović, Predrag and Dronjak, Slađana",
year = "2016",
abstract = "Preclinical studies have shown that melatonin exercised antidepressant-like and anxiolytic-like effects in animal models of anxiety. The aim of the present study was to correlate the changes in behaviour induced by melatonin treatment with the activity of the dopaminergic system in the hippocampus of Wistar rats exposed to chronic, unpredictable, mild stress (CUMS). Male Wistar rats, 11 weeks old, were subjected to chronic stress for 28 successive days. Separate groups of control and stressed rats were intraperitoneally injected daily either with melatonin (10 mg/kg/day, i.p.) or placebo (5% ethanol). The open-field and elevated plus-maze tests were used to assess locomotor activities and anxiety levels. The content of dopamine (DA) in the hippocampal tissues was determined using radioenzymatic assay, while changes in tyrosine hydroxylase (TH) mRNA and protein levels in the hippocampus were determined using real-time RT-PCR and Western immunoblotting. Chronic stress led to reduction in the hippocampal dopaminergic content without affecting the levels of TH protein. These changes were accompanied by increased locomotor activity and higher anxiety levels in the open-field test. Administration of melatonin for 28 days resulted in an increase in the hippocampal DA content as a result of elevated TH protein levels. Melatonin showed an improvement in anxiety-like behaviour along with significantly reduced exploration. We could conclude that melatonin may stimulate dopaminergic synthesis in the hippocampus in order to suppress stress-induced behaviour.",
journal = "Folia Biologica",
title = "Anxiety and Hyperlocomotion Induced by Chronic Unpredictable Mild Stress Can Be Moderated with Melatonin Treatment",
volume = "62",
number = "6",
pages = "250-257",
url = "https://hdl.handle.net/21.15107/rcub_vinar_1495"
}

Spasojević, N., Stefanović, B., Jovanović, P.,& Dronjak, S.. (2016). Anxiety and Hyperlocomotion Induced by Chronic Unpredictable Mild Stress Can Be Moderated with Melatonin Treatment. in Folia Biologica, 62(6), 250-257.
https://hdl.handle.net/21.15107/rcub_vinar_1495

Spasojević N, Stefanović B, Jovanović P, Dronjak S. Anxiety and Hyperlocomotion Induced by Chronic Unpredictable Mild Stress Can Be Moderated with Melatonin Treatment. in Folia Biologica. 2016;62(6):250-257.
https://hdl.handle.net/21.15107/rcub_vinar_1495 .

Spasojević, Nataša, Stefanović, Bojana, Jovanović, Predrag, Dronjak, Slađana, "Anxiety and Hyperlocomotion Induced by Chronic Unpredictable Mild Stress Can Be Moderated with Melatonin Treatment" in Folia Biologica, 62, no. 6 (2016):250-257,
https://hdl.handle.net/21.15107/rcub_vinar_1495 .

TY  - JOUR
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Dronjak, Slađana
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/463
AB  - We have recently shown that chronic fluoxetine treatment acted significantly increasing plasma norepinephrine and epinephrine concentrations both in control and chronically stressed adult male rats. However, possible effects of fluoxetine on catecholamine synthesis and re-uptake in adrenal medulla have been largely unknown. In the present study the effects of chronic fluoxetine treatment on tyrosine hydroxylase, a rate-limiting enzyme in catecholamine synthesis, as well as a norepinephrine transporter and vesicular monoamine transporter 2 gene expressions in adrenal medulla of animals exposed to chronic unpredictable mild stress (CUMS) for 4 weeks, were investigated. Gene expression analyses were performed using a real-time quantitative reverse transcription-PCR. Chronically stressed animals had increased tyrosine hydroxylase mRNA levels and decreased expression of both transporters. Fluoxetine increased tyrosine hydroxylase and decreased norepinephrine transporter gene expression in both unstressed and CUMS rats. These findings suggest that chronic fluoxetine treatment increased plasma catecholamine levels by affecting opposing changes in catecholamine synthesis and uptake.
T2  - Anais de Academia Brasileira de Ciencias
T1  - Differential regulation of catecholamine synthesis and transport in rat adrenal medulla by fluoxetine treatment
VL  - 87
IS  - 1
SP  - 343
EP  - 350
DO  - 10.1590/0001-3765201520130400
ER  - 

@article{
author = "Spasojević, Nataša and Jovanović, Predrag and Dronjak, Slađana",
year = "2015",
abstract = "We have recently shown that chronic fluoxetine treatment acted significantly increasing plasma norepinephrine and epinephrine concentrations both in control and chronically stressed adult male rats. However, possible effects of fluoxetine on catecholamine synthesis and re-uptake in adrenal medulla have been largely unknown. In the present study the effects of chronic fluoxetine treatment on tyrosine hydroxylase, a rate-limiting enzyme in catecholamine synthesis, as well as a norepinephrine transporter and vesicular monoamine transporter 2 gene expressions in adrenal medulla of animals exposed to chronic unpredictable mild stress (CUMS) for 4 weeks, were investigated. Gene expression analyses were performed using a real-time quantitative reverse transcription-PCR. Chronically stressed animals had increased tyrosine hydroxylase mRNA levels and decreased expression of both transporters. Fluoxetine increased tyrosine hydroxylase and decreased norepinephrine transporter gene expression in both unstressed and CUMS rats. These findings suggest that chronic fluoxetine treatment increased plasma catecholamine levels by affecting opposing changes in catecholamine synthesis and uptake.",
journal = "Anais de Academia Brasileira de Ciencias",
title = "Differential regulation of catecholamine synthesis and transport in rat adrenal medulla by fluoxetine treatment",
volume = "87",
number = "1",
pages = "343-350",
doi = "10.1590/0001-3765201520130400"
}

Spasojević, N., Jovanović, P.,& Dronjak, S.. (2015). Differential regulation of catecholamine synthesis and transport in rat adrenal medulla by fluoxetine treatment. in Anais de Academia Brasileira de Ciencias, 87(1), 343-350.
https://doi.org/10.1590/0001-3765201520130400

Spasojević N, Jovanović P, Dronjak S. Differential regulation of catecholamine synthesis and transport in rat adrenal medulla by fluoxetine treatment. in Anais de Academia Brasileira de Ciencias. 2015;87(1):343-350.
doi:10.1590/0001-3765201520130400 .

Spasojević, Nataša, Jovanović, Predrag, Dronjak, Slađana, "Differential regulation of catecholamine synthesis and transport in rat adrenal medulla by fluoxetine treatment" in Anais de Academia Brasileira de Ciencias, 87, no. 1 (2015):343-350,
https://doi.org/10.1590/0001-3765201520130400 . .

TY  - JOUR
AU  - Jovanović, Predrag
AU  - Spasojević, Nataša
AU  - Stefanović, Bojana
AU  - Božović, N.
AU  - Jasnic, N.
AU  - Đorđević, Jelena D.
AU  - Dronjak, Slađana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5825
AB  - The neuropeptide oxytocin has been shown to influence on neuroendocrine function. The aim of the present study was to investigate the effect of peripheral oxytocin treatment on the synthesis, uptake and content of adreno-medullary catecholamine. For this purpose oxytocin (3.6 mu g/100 g body weight, s.c) was administrated to male rats once a day over 14 days. In order to assess the effect of peripheral oxytocin treatment on adreno-medullary catecholamine we measured epinephrine and norepinephrine content and gene expression of tyrosine hydroxylase (TH), norepinephrine transporter (NET) and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. Our results show a significant increase of epinephrine (1.7-fold, p LT 0.05) and norepinephrine (1.5-fold, p LT 0.05) content in oxytocin treated animals compared to saline treated ones. Oxytocin treatment had no effect either on mRNA or protein level of TH and NET. Under oxytocin treatment the increase in VMAT2 mRNA level was not statistically significant, but it caused a significant increase in protein level of VMAT2 (3.7-fold, p LT 0.001). These findings indicate that oxytocin treatment increases catecholamine content in the rat adrenal medulla modulating VMAT2 expression. (C) 2013 Elsevier Inc. All rights reserved.
T2  - Peptides
T1  - Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2
VL  - 51
SP  - 110
EP  - 114
DO  - 10.1016/j.peptides.2013.11.001
ER  - 

@article{
author = "Jovanović, Predrag and Spasojević, Nataša and Stefanović, Bojana and Božović, N. and Jasnic, N. and Đorđević, Jelena D. and Dronjak, Slađana",
year = "2014",
abstract = "The neuropeptide oxytocin has been shown to influence on neuroendocrine function. The aim of the present study was to investigate the effect of peripheral oxytocin treatment on the synthesis, uptake and content of adreno-medullary catecholamine. For this purpose oxytocin (3.6 mu g/100 g body weight, s.c) was administrated to male rats once a day over 14 days. In order to assess the effect of peripheral oxytocin treatment on adreno-medullary catecholamine we measured epinephrine and norepinephrine content and gene expression of tyrosine hydroxylase (TH), norepinephrine transporter (NET) and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. Our results show a significant increase of epinephrine (1.7-fold, p LT 0.05) and norepinephrine (1.5-fold, p LT 0.05) content in oxytocin treated animals compared to saline treated ones. Oxytocin treatment had no effect either on mRNA or protein level of TH and NET. Under oxytocin treatment the increase in VMAT2 mRNA level was not statistically significant, but it caused a significant increase in protein level of VMAT2 (3.7-fold, p LT 0.001). These findings indicate that oxytocin treatment increases catecholamine content in the rat adrenal medulla modulating VMAT2 expression. (C) 2013 Elsevier Inc. All rights reserved.",
journal = "Peptides",
title = "Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2",
volume = "51",
pages = "110-114",
doi = "10.1016/j.peptides.2013.11.001"
}

Jovanović, P., Spasojević, N., Stefanović, B., Božović, N., Jasnic, N., Đorđević, J. D.,& Dronjak, S.. (2014). Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2. in Peptides, 51, 110-114.
https://doi.org/10.1016/j.peptides.2013.11.001

Jovanović P, Spasojević N, Stefanović B, Božović N, Jasnic N, Đorđević JD, Dronjak S. Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2. in Peptides. 2014;51:110-114.
doi:10.1016/j.peptides.2013.11.001 .

Jovanović, Predrag, Spasojević, Nataša, Stefanović, Bojana, Božović, N., Jasnic, N., Đorđević, Jelena D., Dronjak, Slađana, "Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2" in Peptides, 51 (2014):110-114,
https://doi.org/10.1016/j.peptides.2013.11.001 . .

TY  - JOUR
AU  - Jovanović, Predrag
AU  - Spasojević, Nataša
AU  - Stefanović, Bojana
AU  - Dronjak, Slađana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5941
AB  - Chronic isolation stress of adult rat males acted increasing gene expression of tyrosine hydroxylase (TH) and neuronal norepinephrine transporter (NET) in the right stellate ganglia, while vesicular monoamine transporter 2 (VMAT2) level remained unchanged. The stress decreased protein level of TH, as well as mRNA levels for NET and VMAT2 in the left stellate ganglia, but expressed no effect on protein levels of these two transporters. These results demonstrate asymmetry in noradrenegic genes in the right and left stellate ganglia during stress and provide molecular evidence to help explain the difference in response to the stress. (C) 2014 Elsevier B.V. All rights reserved.
T2  - Autonomic Neuroscience: Basic and Clinical
T1  - Differential expression of tyrosine hydroxylase and transporters in the right and left stellate ganglion of socially isolated rats
VL  - 181
SP  - 85
EP  - 89
DO  - 10.1016/j.autneu.2014.01.002
ER  - 

@article{
author = "Jovanović, Predrag and Spasojević, Nataša and Stefanović, Bojana and Dronjak, Slađana",
year = "2014",
abstract = "Chronic isolation stress of adult rat males acted increasing gene expression of tyrosine hydroxylase (TH) and neuronal norepinephrine transporter (NET) in the right stellate ganglia, while vesicular monoamine transporter 2 (VMAT2) level remained unchanged. The stress decreased protein level of TH, as well as mRNA levels for NET and VMAT2 in the left stellate ganglia, but expressed no effect on protein levels of these two transporters. These results demonstrate asymmetry in noradrenegic genes in the right and left stellate ganglia during stress and provide molecular evidence to help explain the difference in response to the stress. (C) 2014 Elsevier B.V. All rights reserved.",
journal = "Autonomic Neuroscience: Basic and Clinical",
title = "Differential expression of tyrosine hydroxylase and transporters in the right and left stellate ganglion of socially isolated rats",
volume = "181",
pages = "85-89",
doi = "10.1016/j.autneu.2014.01.002"
}

Jovanović, P., Spasojević, N., Stefanović, B.,& Dronjak, S.. (2014). Differential expression of tyrosine hydroxylase and transporters in the right and left stellate ganglion of socially isolated rats. in Autonomic Neuroscience: Basic and Clinical, 181, 85-89.
https://doi.org/10.1016/j.autneu.2014.01.002

Jovanović P, Spasojević N, Stefanović B, Dronjak S. Differential expression of tyrosine hydroxylase and transporters in the right and left stellate ganglion of socially isolated rats. in Autonomic Neuroscience: Basic and Clinical. 2014;181:85-89.
doi:10.1016/j.autneu.2014.01.002 .

Jovanović, Predrag, Spasojević, Nataša, Stefanović, Bojana, Dronjak, Slađana, "Differential expression of tyrosine hydroxylase and transporters in the right and left stellate ganglion of socially isolated rats" in Autonomic Neuroscience: Basic and Clinical, 181 (2014):85-89,
https://doi.org/10.1016/j.autneu.2014.01.002 . .

TY  - JOUR
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Stefanović, Bojana
AU  - Dronjak, Slađana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/227
AB  - Social isolation produce various neurochemical alterations in monoamines pathways of the brain. In order to assess the effect of individual housing of adult rats on neurochemical substrates and lateralization in the right and left medial prefrontal cortex (mPFC), we measured dopamine and noradrenaline stores, protein levels of tyrosine-hydroxylase (TH), noradrenaline transporter (NET) and vesicular monoamine transporter 2 (VMAT 2), by Western blot analysis. Individual housing produced no significant changes in dopamine and noradrenaline content, whereas levels of TH and NET were elevated in the right and left mPFC. On the other hand, VMAT 2 protein was reduced in the right mPFC and increased in the left mPFC. The results indicate an increased synthetic and reuptake capacity of noradrenergic terminals in the right and left mPFC and decreased translocation monoamines from the cytoplasm into the intracellular storage vesicles of neurons in the right mPFC.
T2  - Neurochemical Journal
T1  - Influence of individual housing in the adult rats on catecholamine synthesis and storage in the right and left medial prefrontal cortex
VL  - 8
IS  - 4
SP  - 277
EP  - 281
DO  - 10.1134/S1819712414040084
ER  - 

@article{
author = "Spasojević, Nataša and Jovanović, Predrag and Stefanović, Bojana and Dronjak, Slađana",
year = "2014",
abstract = "Social isolation produce various neurochemical alterations in monoamines pathways of the brain. In order to assess the effect of individual housing of adult rats on neurochemical substrates and lateralization in the right and left medial prefrontal cortex (mPFC), we measured dopamine and noradrenaline stores, protein levels of tyrosine-hydroxylase (TH), noradrenaline transporter (NET) and vesicular monoamine transporter 2 (VMAT 2), by Western blot analysis. Individual housing produced no significant changes in dopamine and noradrenaline content, whereas levels of TH and NET were elevated in the right and left mPFC. On the other hand, VMAT 2 protein was reduced in the right mPFC and increased in the left mPFC. The results indicate an increased synthetic and reuptake capacity of noradrenergic terminals in the right and left mPFC and decreased translocation monoamines from the cytoplasm into the intracellular storage vesicles of neurons in the right mPFC.",
journal = "Neurochemical Journal",
title = "Influence of individual housing in the adult rats on catecholamine synthesis and storage in the right and left medial prefrontal cortex",
volume = "8",
number = "4",
pages = "277-281",
doi = "10.1134/S1819712414040084"
}

Spasojević, N., Jovanović, P., Stefanović, B.,& Dronjak, S.. (2014). Influence of individual housing in the adult rats on catecholamine synthesis and storage in the right and left medial prefrontal cortex. in Neurochemical Journal, 8(4), 277-281.
https://doi.org/10.1134/S1819712414040084

Spasojević N, Jovanović P, Stefanović B, Dronjak S. Influence of individual housing in the adult rats on catecholamine synthesis and storage in the right and left medial prefrontal cortex. in Neurochemical Journal. 2014;8(4):277-281.
doi:10.1134/S1819712414040084 .

Spasojević, Nataša, Jovanović, Predrag, Stefanović, Bojana, Dronjak, Slađana, "Influence of individual housing in the adult rats on catecholamine synthesis and storage in the right and left medial prefrontal cortex" in Neurochemical Journal, 8, no. 4 (2014):277-281,
https://doi.org/10.1134/S1819712414040084 . .

TY  - JOUR
AU  - Jovanović, Predrag
AU  - Puškaš, Nela
AU  - Stefanović, Bojana
AU  - Spasojević, Nataša
AU  - Dronjak, Slađana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/300
AB  - Social isolation induced a significant increase in resting heart rate and reduction in heart rate variability. Dysfunction of the intrinsic cardiac nervous system is implicated in the genesis of cardiovascular diseases. Previous evidence suggests that cardiac ganglia contain noradrenergic neurons. Thus, immunohistochemical expression of catecholamine-synthesizing enzyme tyrosine hydroxylase (TH) and vesicular monoamine transporter 2 (VMAT2) were analyzed, as well as the effects of social isolation stress on mRNA and protein levels of this enzyme and transporter in the intrinsic cardiac nervous system of adult rats. Our results indicate that cardiac ganglion neurons express TH and VMAT2 immunoreactivity. Chronic isolated stress of rats caused a decrease in TH mRNA and VMAT2 mRNA in the neurons of intrinsic cardiac ganglia. No significant alterations in the protein levels of TH and VMAT2 were observed in these neurons. These data indicate that the neurons of intrinsic cardiac ganglia express TH as well as VMAT2 but that social isolation stress does not change their protein levels.
T2  - Archives of Biological Sciences
T1  - Immunohistochemical Detection and Gene Expression of Tyrosine Hydroxylase and Vesicular Monoamine Transporter Type 2 in Intrinsic Cardiac Ganglia of Socially Isolated Rats
VL  - 66
IS  - 4
SP  - 1645
EP  - 1651
DO  - 10.2298/ABS1404645J
ER  - 

@article{
author = "Jovanović, Predrag and Puškaš, Nela and Stefanović, Bojana and Spasojević, Nataša and Dronjak, Slađana",
year = "2014",
abstract = "Social isolation induced a significant increase in resting heart rate and reduction in heart rate variability. Dysfunction of the intrinsic cardiac nervous system is implicated in the genesis of cardiovascular diseases. Previous evidence suggests that cardiac ganglia contain noradrenergic neurons. Thus, immunohistochemical expression of catecholamine-synthesizing enzyme tyrosine hydroxylase (TH) and vesicular monoamine transporter 2 (VMAT2) were analyzed, as well as the effects of social isolation stress on mRNA and protein levels of this enzyme and transporter in the intrinsic cardiac nervous system of adult rats. Our results indicate that cardiac ganglion neurons express TH and VMAT2 immunoreactivity. Chronic isolated stress of rats caused a decrease in TH mRNA and VMAT2 mRNA in the neurons of intrinsic cardiac ganglia. No significant alterations in the protein levels of TH and VMAT2 were observed in these neurons. These data indicate that the neurons of intrinsic cardiac ganglia express TH as well as VMAT2 but that social isolation stress does not change their protein levels.",
journal = "Archives of Biological Sciences",
title = "Immunohistochemical Detection and Gene Expression of Tyrosine Hydroxylase and Vesicular Monoamine Transporter Type 2 in Intrinsic Cardiac Ganglia of Socially Isolated Rats",
volume = "66",
number = "4",
pages = "1645-1651",
doi = "10.2298/ABS1404645J"
}

Jovanović, P., Puškaš, N., Stefanović, B., Spasojević, N.,& Dronjak, S.. (2014). Immunohistochemical Detection and Gene Expression of Tyrosine Hydroxylase and Vesicular Monoamine Transporter Type 2 in Intrinsic Cardiac Ganglia of Socially Isolated Rats. in Archives of Biological Sciences, 66(4), 1645-1651.
https://doi.org/10.2298/ABS1404645J

Jovanović P, Puškaš N, Stefanović B, Spasojević N, Dronjak S. Immunohistochemical Detection and Gene Expression of Tyrosine Hydroxylase and Vesicular Monoamine Transporter Type 2 in Intrinsic Cardiac Ganglia of Socially Isolated Rats. in Archives of Biological Sciences. 2014;66(4):1645-1651.
doi:10.2298/ABS1404645J .

Jovanović, Predrag, Puškaš, Nela, Stefanović, Bojana, Spasojević, Nataša, Dronjak, Slađana, "Immunohistochemical Detection and Gene Expression of Tyrosine Hydroxylase and Vesicular Monoamine Transporter Type 2 in Intrinsic Cardiac Ganglia of Socially Isolated Rats" in Archives of Biological Sciences, 66, no. 4 (2014):1645-1651,
https://doi.org/10.2298/ABS1404645J . .

TY  - JOUR
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Stanisavljevic, D.
AU  - Stefanović, Bojana
AU  - Dronjak, Slađana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5546
AB  - The brain monoamine systems play a crucial role in mediating social behaviors. In order to assess the effect of socially isolated rats on neurochemical substrates in hypothalamus we measured levels of tyrosine hydroxylase (TH), noradrenaline transporter (NET) and vesicular monoamine transporter 2 (VMAT 2). Our results show that 12 weeks of social isolation produced no significant changes in noradrenaline content in hypothalamus. The levels of TH and NET were elevated in these animals, whereas level of VMAT 2 was unchanged. The data suggests that both enhanced synthesis and reuptake may aid in the recovery of noradrenaline content in the hypothalamus. The adaptation of the hypothalamic noradrenaline to long period of stress may have great physiological importance and may represent mechanism designed to help the organism adapt to the deleterious effects of chronic indiviual housing.
T2  - Neurochemical Journal
T1  - Hypothalamic noradrenaline synthesis, uptake and storage in rats during adaptation to long-term individual housing
VL  - 7
IS  - 2
SP  - 139
EP  - 143
DO  - 10.1134/S1819712413020050
ER  - 

@article{
author = "Spasojević, Nataša and Jovanović, Predrag and Stanisavljevic, D. and Stefanović, Bojana and Dronjak, Slađana",
year = "2013",
abstract = "The brain monoamine systems play a crucial role in mediating social behaviors. In order to assess the effect of socially isolated rats on neurochemical substrates in hypothalamus we measured levels of tyrosine hydroxylase (TH), noradrenaline transporter (NET) and vesicular monoamine transporter 2 (VMAT 2). Our results show that 12 weeks of social isolation produced no significant changes in noradrenaline content in hypothalamus. The levels of TH and NET were elevated in these animals, whereas level of VMAT 2 was unchanged. The data suggests that both enhanced synthesis and reuptake may aid in the recovery of noradrenaline content in the hypothalamus. The adaptation of the hypothalamic noradrenaline to long period of stress may have great physiological importance and may represent mechanism designed to help the organism adapt to the deleterious effects of chronic indiviual housing.",
journal = "Neurochemical Journal",
title = "Hypothalamic noradrenaline synthesis, uptake and storage in rats during adaptation to long-term individual housing",
volume = "7",
number = "2",
pages = "139-143",
doi = "10.1134/S1819712413020050"
}

Spasojević, N., Jovanović, P., Stanisavljevic, D., Stefanović, B.,& Dronjak, S.. (2013). Hypothalamic noradrenaline synthesis, uptake and storage in rats during adaptation to long-term individual housing. in Neurochemical Journal, 7(2), 139-143.
https://doi.org/10.1134/S1819712413020050

Spasojević N, Jovanović P, Stanisavljevic D, Stefanović B, Dronjak S. Hypothalamic noradrenaline synthesis, uptake and storage in rats during adaptation to long-term individual housing. in Neurochemical Journal. 2013;7(2):139-143.
doi:10.1134/S1819712413020050 .

Spasojević, Nataša, Jovanović, Predrag, Stanisavljevic, D., Stefanović, Bojana, Dronjak, Slađana, "Hypothalamic noradrenaline synthesis, uptake and storage in rats during adaptation to long-term individual housing" in Neurochemical Journal, 7, no. 2 (2013):139-143,
https://doi.org/10.1134/S1819712413020050 . .

TY  - JOUR
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Dronjak, Slađana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5810
AB  - Neurochemical lateralization has been demonstrated in the rat brain suggesting that such lateralization might contribute to behavior. Thus, the aim of the present study was to examine neurochemical asymmetry in the hippocampus, molecular basis of neurochemical lateralization and its impact on spatial learning and memory. Changes in noradrenaline content, tyrosine hydroxylase (TH) were studied in the right and left hippocampus of naive control and chronically isolated rats, by applying TaqMan RT-PCR and Western blot analysis. Hippocampal-based spatial learning and memory were evaluated using the Barnes maze. In control rats an asymmetrical right-left distribution of noradrenaline content and gene expression of catecholamine synthesizing enzyme was found. Chronic psychosocial stress further emphasized asymmetry. Isolation stress reduced noradrenaline content only in the right hippocampus. No changes were observed in gene expression and protein levels of TH in the right hippocampus, whereas expression of catecholamine synthesizing enzyme was elevated in the left hippocampus. Reduced noradrenaline content in the right hippocampus did not cause impairment in spatial learning and memory. Our findings suggest that chronic psychosocial stress reduces noradrenaline stores in the right hippocampus which may be caused by molecular asymmetry, but it does not affect spatial learning and memory.
T2  - Acta Physiologica Hungarica
T1  - Molecular basis of chronic stress-induced hippocampal lateral asymmetry in rats and impact on learning and memory
VL  - 100
IS  - 4
SP  - 388
EP  - 394
DO  - 10.1556/APhysiol.100.2013.4.3
ER  - 

@article{
author = "Spasojević, Nataša and Jovanović, Predrag and Dronjak, Slađana",
year = "2013",
abstract = "Neurochemical lateralization has been demonstrated in the rat brain suggesting that such lateralization might contribute to behavior. Thus, the aim of the present study was to examine neurochemical asymmetry in the hippocampus, molecular basis of neurochemical lateralization and its impact on spatial learning and memory. Changes in noradrenaline content, tyrosine hydroxylase (TH) were studied in the right and left hippocampus of naive control and chronically isolated rats, by applying TaqMan RT-PCR and Western blot analysis. Hippocampal-based spatial learning and memory were evaluated using the Barnes maze. In control rats an asymmetrical right-left distribution of noradrenaline content and gene expression of catecholamine synthesizing enzyme was found. Chronic psychosocial stress further emphasized asymmetry. Isolation stress reduced noradrenaline content only in the right hippocampus. No changes were observed in gene expression and protein levels of TH in the right hippocampus, whereas expression of catecholamine synthesizing enzyme was elevated in the left hippocampus. Reduced noradrenaline content in the right hippocampus did not cause impairment in spatial learning and memory. Our findings suggest that chronic psychosocial stress reduces noradrenaline stores in the right hippocampus which may be caused by molecular asymmetry, but it does not affect spatial learning and memory.",
journal = "Acta Physiologica Hungarica",
title = "Molecular basis of chronic stress-induced hippocampal lateral asymmetry in rats and impact on learning and memory",
volume = "100",
number = "4",
pages = "388-394",
doi = "10.1556/APhysiol.100.2013.4.3"
}

Spasojević, N., Jovanović, P.,& Dronjak, S.. (2013). Molecular basis of chronic stress-induced hippocampal lateral asymmetry in rats and impact on learning and memory. in Acta Physiologica Hungarica, 100(4), 388-394.
https://doi.org/10.1556/APhysiol.100.2013.4.3

Spasojević N, Jovanović P, Dronjak S. Molecular basis of chronic stress-induced hippocampal lateral asymmetry in rats and impact on learning and memory. in Acta Physiologica Hungarica. 2013;100(4):388-394.
doi:10.1556/APhysiol.100.2013.4.3 .

Spasojević, Nataša, Jovanović, Predrag, Dronjak, Slađana, "Molecular basis of chronic stress-induced hippocampal lateral asymmetry in rats and impact on learning and memory" in Acta Physiologica Hungarica, 100, no. 4 (2013):388-394,
https://doi.org/10.1556/APhysiol.100.2013.4.3 . .

TY  - JOUR
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Dronjak, Slađana
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5153
AB  - Depression is associated with increased risk of coronary heart diseases. Selective serotonin reuptake inhibitors (SSRIs) have been proved to be very effective in normalizing symptoms of depression, but the data on possible influence of these drugs on cardiovascular function is controversial. Applying Taqman RT-PCR assay, the effect of chronic treatment with a SSRI antidepressant fluoxetine has been investigated on gene expression of catecholamine biosynthetic enzymes in all four heart chambers of rats with signs of depression. Depression was induced by exposing the animals to chronic unpredictable mild stress (CUMS). Tyrosine-hydroxylase (TH) and dopamine-beta-hydroxylase (DBH) mRNA levels were decreased both in right and left atria, while phenylethanolamine N-methyltransferase (PNMT) mRNAs were increased in left atria and both ventricles of depression model rats. Fluoxetine elevated gene expression of TH and DBH in atria, but did not influence this process in the ventricles. Also, this antidepressant did not express a significant effect on the level of PNMT mRNA both in atria and ventricles. These results indicate that fluoxetine acted stimulating noradrenaline synthesis in the heart, which could lead to increased risk of heart disease.
T2  - Indian Journal of Experimental Biology
T1  - Chronic fluoxetine treatment affects gene expression of catecholamine enzymes in the heart of depression model rats
VL  - 50
IS  - 11
SP  - 771
EP  - 775
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5153
ER  - 

@article{
author = "Spasojević, Nataša and Jovanović, Predrag and Dronjak, Slađana",
year = "2012",
abstract = "Depression is associated with increased risk of coronary heart diseases. Selective serotonin reuptake inhibitors (SSRIs) have been proved to be very effective in normalizing symptoms of depression, but the data on possible influence of these drugs on cardiovascular function is controversial. Applying Taqman RT-PCR assay, the effect of chronic treatment with a SSRI antidepressant fluoxetine has been investigated on gene expression of catecholamine biosynthetic enzymes in all four heart chambers of rats with signs of depression. Depression was induced by exposing the animals to chronic unpredictable mild stress (CUMS). Tyrosine-hydroxylase (TH) and dopamine-beta-hydroxylase (DBH) mRNA levels were decreased both in right and left atria, while phenylethanolamine N-methyltransferase (PNMT) mRNAs were increased in left atria and both ventricles of depression model rats. Fluoxetine elevated gene expression of TH and DBH in atria, but did not influence this process in the ventricles. Also, this antidepressant did not express a significant effect on the level of PNMT mRNA both in atria and ventricles. These results indicate that fluoxetine acted stimulating noradrenaline synthesis in the heart, which could lead to increased risk of heart disease.",
journal = "Indian Journal of Experimental Biology",
title = "Chronic fluoxetine treatment affects gene expression of catecholamine enzymes in the heart of depression model rats",
volume = "50",
number = "11",
pages = "771-775",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5153"
}

Spasojević, N., Jovanović, P.,& Dronjak, S.. (2012). Chronic fluoxetine treatment affects gene expression of catecholamine enzymes in the heart of depression model rats. in Indian Journal of Experimental Biology, 50(11), 771-775.
https://hdl.handle.net/21.15107/rcub_vinar_5153

Spasojević N, Jovanović P, Dronjak S. Chronic fluoxetine treatment affects gene expression of catecholamine enzymes in the heart of depression model rats. in Indian Journal of Experimental Biology. 2012;50(11):771-775.
https://hdl.handle.net/21.15107/rcub_vinar_5153 .

Spasojević, Nataša, Jovanović, Predrag, Dronjak, Slađana, "Chronic fluoxetine treatment affects gene expression of catecholamine enzymes in the heart of depression model rats" in Indian Journal of Experimental Biology, 50, no. 11 (2012):771-775,
https://hdl.handle.net/21.15107/rcub_vinar_5153 .