TY  - CONF
AU  - Čolović, Mirjana
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Savić, Nada
AU  - Parac-Vogt, Tatjana
AU  - Krstić, Danijela
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12944
AB  - The aim of this study was to in vitro assess the anti-tumor potential of Fe(III)-substituted monolacunary Wells-Dawson polyoxotungstate, K7[FeIII(α2-P2W17O61)(H2O)] (FeWD) against human melanoma A375 cells. A375 cells were treated in vitro with FeWD in the concentration range of 0.001-1 mM, for 24, 48, and 72 hours. FeWD decreased A375 cell viability in a dose- and time-dependent manner. IC50 values (in mM), as a marker of the cytotoxic potency of FeWD, were obtained as follows: 1, 0.58, and 0.51, for 24-, 48-, and 72-hour exposure, respectively. However, in comparison with cisplatin as a gold standard in cancer chemotherapy, which was used as a positive control, IC50 values (in mM) were significantly higher than those obtained for cisplatin (0.09, 0.07, and 0.043, for 24, 48, and 72 hours, respectively). For this reason, the studied FeWD polyoxometalate could not be considered a superior anti-cancer candidate compared to the standard chemotherapeutic.
C3  - International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings
T1  - Anti-human melanoma effect of Fe(III)-containing wells-dawson nanocluster in vitro
VL  - 2
SP  - 24
EP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12944
ER  - 

@conference{
author = "Čolović, Mirjana and Korićanac, Lela and Žakula, Jelena and Savić, Nada and Parac-Vogt, Tatjana and Krstić, Danijela",
year = "2024",
abstract = "The aim of this study was to in vitro assess the anti-tumor potential of Fe(III)-substituted monolacunary Wells-Dawson polyoxotungstate, K7[FeIII(α2-P2W17O61)(H2O)] (FeWD) against human melanoma A375 cells. A375 cells were treated in vitro with FeWD in the concentration range of 0.001-1 mM, for 24, 48, and 72 hours. FeWD decreased A375 cell viability in a dose- and time-dependent manner. IC50 values (in mM), as a marker of the cytotoxic potency of FeWD, were obtained as follows: 1, 0.58, and 0.51, for 24-, 48-, and 72-hour exposure, respectively. However, in comparison with cisplatin as a gold standard in cancer chemotherapy, which was used as a positive control, IC50 values (in mM) were significantly higher than those obtained for cisplatin (0.09, 0.07, and 0.043, for 24, 48, and 72 hours, respectively). For this reason, the studied FeWD polyoxometalate could not be considered a superior anti-cancer candidate compared to the standard chemotherapeutic.",
journal = "International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings",
title = "Anti-human melanoma effect of Fe(III)-containing wells-dawson nanocluster in vitro",
volume = "2",
pages = "24-27",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12944"
}

Čolović, M., Korićanac, L., Žakula, J., Savić, N., Parac-Vogt, T.,& Krstić, D.. (2024). Anti-human melanoma effect of Fe(III)-containing wells-dawson nanocluster in vitro. in International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings, 2, 24-27.
https://hdl.handle.net/21.15107/rcub_vinar_12944

Čolović M, Korićanac L, Žakula J, Savić N, Parac-Vogt T, Krstić D. Anti-human melanoma effect of Fe(III)-containing wells-dawson nanocluster in vitro. in International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings. 2024;2:24-27.
https://hdl.handle.net/21.15107/rcub_vinar_12944 .

Čolović, Mirjana, Korićanac, Lela, Žakula, Jelena, Savić, Nada, Parac-Vogt, Tatjana, Krstić, Danijela, "Anti-human melanoma effect of Fe(III)-containing wells-dawson nanocluster in vitro" in International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings, 2 (2024):24-27,
https://hdl.handle.net/21.15107/rcub_vinar_12944 .

TY  - CONF
AU  - Krstić, Danijela
AU  - Lalatović, Jovana
AU  - Mrđa, Davor
AU  - Stojanović, Marko
AU  - Savić, Nada
AU  - Parac-Vogt, Tatjana
AU  - Čolović, Mirjana
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12945
AB  - The purpose of this study was to evaluate in vitro contrast properties of a synthesized Wells-Dawson P2W17O61)2]·19H2O (Hf-WD 1:2), as a potential contrast agent for computed tomography (CT). X-ray attenuation was determined in vitro in the presence of increasing tungsten concentrations (3.125-100 mM) of Hf-WD 1:2. In order to compare the contrast properties of the tungsten-containing Hf-WD 1:2 with a commercially available CT contrast agent that has been used in clinical practice, X-ray attenuation was presented for the same iodine concentrations of iodine-based iohexol solution as well. Higher HU values for particular concentrations were obtained for Hf-WD 1:2 in comparison with those determined for the standard iohexol. Thus, the studied Hf-WD 1:2 POM could be considered a promising contrast agent candidate for CT. Nevertheless, further development of Hf-WD 1:2 as a potential CT contrast requires additional CT studies in vivo and relevant toxicity studies in vitro and in vivo as well.
C3  - International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings
T1  - Hafnium-containing Wells-Dawson nanocluster as a promising contrast agent candidate
VL  - 2
SP  - 39
EP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12945
ER  - 

@conference{
author = "Krstić, Danijela and Lalatović, Jovana and Mrđa, Davor and Stojanović, Marko and Savić, Nada and Parac-Vogt, Tatjana and Čolović, Mirjana",
year = "2024",
abstract = "The purpose of this study was to evaluate in vitro contrast properties of a synthesized Wells-Dawson P2W17O61)2]·19H2O (Hf-WD 1:2), as a potential contrast agent for computed tomography (CT). X-ray attenuation was determined in vitro in the presence of increasing tungsten concentrations (3.125-100 mM) of Hf-WD 1:2. In order to compare the contrast properties of the tungsten-containing Hf-WD 1:2 with a commercially available CT contrast agent that has been used in clinical practice, X-ray attenuation was presented for the same iodine concentrations of iodine-based iohexol solution as well. Higher HU values for particular concentrations were obtained for Hf-WD 1:2 in comparison with those determined for the standard iohexol. Thus, the studied Hf-WD 1:2 POM could be considered a promising contrast agent candidate for CT. Nevertheless, further development of Hf-WD 1:2 as a potential CT contrast requires additional CT studies in vivo and relevant toxicity studies in vitro and in vivo as well.",
journal = "International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings",
title = "Hafnium-containing Wells-Dawson nanocluster as a promising contrast agent candidate",
volume = "2",
pages = "39-42",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12945"
}

Krstić, D., Lalatović, J., Mrđa, D., Stojanović, M., Savić, N., Parac-Vogt, T.,& Čolović, M.. (2024). Hafnium-containing Wells-Dawson nanocluster as a promising contrast agent candidate. in International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings, 2, 39-42.
https://hdl.handle.net/21.15107/rcub_vinar_12945

Krstić D, Lalatović J, Mrđa D, Stojanović M, Savić N, Parac-Vogt T, Čolović M. Hafnium-containing Wells-Dawson nanocluster as a promising contrast agent candidate. in International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings. 2024;2:39-42.
https://hdl.handle.net/21.15107/rcub_vinar_12945 .

Krstić, Danijela, Lalatović, Jovana, Mrđa, Davor, Stojanović, Marko, Savić, Nada, Parac-Vogt, Tatjana, Čolović, Mirjana, "Hafnium-containing Wells-Dawson nanocluster as a promising contrast agent candidate" in International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings, 2 (2024):39-42,
https://hdl.handle.net/21.15107/rcub_vinar_12945 .

TY  - CONF
AU  - Čolović, Mirjana
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Savić, Nada
AU  - Parac-Vogt, Tatjana
AU  - Krstić, Danijela Z.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12927
AB  - The aim of this study was to assess in vitro cytotoxic activity of a monolacunary Wells- Dawson nanocluster, α2-K10P2W17O61.20H2O (lacunary WD) against cervical carcinoma HeLa cells  as a commonly used model system for the evaluation of antitumor properties. After HeLa cells had been exposed to the investigated polyoxotungstate (the concentration range of 0.001 - 1 mM) for 24, 48, and 72 h, relative cell viability (expressed as a percentage of control) was determined.  The obtained results showed that lacunary WD affected HeLa cell viability in a concentration- and time-dependent manner. IC50 values (in μM), calculated using sigmoidal fitting experimental  plots, were as follows: 24.11 ± 9.95, 12.74 ± 0.096, and 11.48 ± 0.12 for 24, 48, and 72 hours treatment, respectively. In comparison with cisplatin, (positive control), IC50 values (μM) for 24 hours treatment were similar – 24.11 (lacunary WD) vs. 24.49 (cisplatin). However, after 48 and 72 hours IC50 obtained for cisplatin were found to be lower – 8.81 and 4.93 μM, respectively. Accordingly, the studied WD polyoxotungstate could not be regarded as a superior anticancer agent in comparison with the standard chemotherapeutic. Nevertheless, this studied nanocluster deserves attention as a promising antitumor therapeutic and as a good platform for the design of next-generation metal-based anticancer agents.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - In vitro cytotoxic activity of a monolacunary Wells-Dawson nanocluster against cervical carcinoma HeLa cells
SP  - 415
EP  - 418
DO  - 10.46793/ICCBI23.415C
ER  - 

@conference{
author = "Čolović, Mirjana and Žakula, Jelena and Korićanac, Lela and Savić, Nada and Parac-Vogt, Tatjana and Krstić, Danijela Z.",
year = "2023",
abstract = "The aim of this study was to assess in vitro cytotoxic activity of a monolacunary Wells- Dawson nanocluster, α2-K10P2W17O61.20H2O (lacunary WD) against cervical carcinoma HeLa cells  as a commonly used model system for the evaluation of antitumor properties. After HeLa cells had been exposed to the investigated polyoxotungstate (the concentration range of 0.001 - 1 mM) for 24, 48, and 72 h, relative cell viability (expressed as a percentage of control) was determined.  The obtained results showed that lacunary WD affected HeLa cell viability in a concentration- and time-dependent manner. IC50 values (in μM), calculated using sigmoidal fitting experimental  plots, were as follows: 24.11 ± 9.95, 12.74 ± 0.096, and 11.48 ± 0.12 for 24, 48, and 72 hours treatment, respectively. In comparison with cisplatin, (positive control), IC50 values (μM) for 24 hours treatment were similar – 24.11 (lacunary WD) vs. 24.49 (cisplatin). However, after 48 and 72 hours IC50 obtained for cisplatin were found to be lower – 8.81 and 4.93 μM, respectively. Accordingly, the studied WD polyoxotungstate could not be regarded as a superior anticancer agent in comparison with the standard chemotherapeutic. Nevertheless, this studied nanocluster deserves attention as a promising antitumor therapeutic and as a good platform for the design of next-generation metal-based anticancer agents.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "In vitro cytotoxic activity of a monolacunary Wells-Dawson nanocluster against cervical carcinoma HeLa cells",
pages = "415-418",
doi = "10.46793/ICCBI23.415C"
}

Čolović, M., Žakula, J., Korićanac, L., Savić, N., Parac-Vogt, T.,& Krstić, D. Z.. (2023). In vitro cytotoxic activity of a monolacunary Wells-Dawson nanocluster against cervical carcinoma HeLa cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 415-418.
https://doi.org/10.46793/ICCBI23.415C

Čolović M, Žakula J, Korićanac L, Savić N, Parac-Vogt T, Krstić DZ. In vitro cytotoxic activity of a monolacunary Wells-Dawson nanocluster against cervical carcinoma HeLa cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:415-418.
doi:10.46793/ICCBI23.415C .

Čolović, Mirjana, Žakula, Jelena, Korićanac, Lela, Savić, Nada, Parac-Vogt, Tatjana, Krstić, Danijela Z., "In vitro cytotoxic activity of a monolacunary Wells-Dawson nanocluster against cervical carcinoma HeLa cells" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):415-418,
https://doi.org/10.46793/ICCBI23.415C . .

TY  - CONF
AU  - Čolović, Mirjana
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Savić, Nada
AU  - Parac-Vogt, Tatjana
AU  - Krstić, Danijela Z.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12928
AB  - The objective of this study was to evaluate in vitro the antitumor properties of Fe(III)- substituted monolacunary Wells-Dawson polyoxotungstate, K7[FeIII(α2-P2W17O61)(H2O)] (FeWD) using cervical carcinoma HeLa cells as a model system. HeLa cells were exposed in vitro to FeWD within the concentration range from 0.001 to 1 mM, for 24, 48, and 72 hours. The studied Fe(III)- substituted polyoxotungstate affected HeLa cell viability in a concentration- and time-dependent manner. The obtained IC50 values (µM), as an indicator of the cytotoxic potential of FeWD, were: 16.64 ± 0.49, 10.75 ± 0.97, and 9.64 ± 0.19 for 24-, 48-, and 72-hour treatment, respectively. FeWD exhibited a stronger antitumor potential against HeLa cells than the structurally similar monolacunary Wells-Dawson polyoxotungstate, K10P2W17O61.20H2O (lacunary WD). Lacunary WD achieved IC50 at 24,11 µM after 24-hour exposure, which is about 44% higher concentration compared to the corresponding IC50 obtained for FeWD. This indicates that incorporating Fe(III) might be a new strategy for improving the antitumor efficacy of polyoxometalates as promising candidates for next-generation chemotherapeutics.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - The influence of Fe(III) incorporation on anti-cancer potential of a Wells-Dawson nanocluster
SP  - 419
EP  - 422
DO  - 10.46793/ICCBI23.419C
ER  - 

@conference{
author = "Čolović, Mirjana and Korićanac, Lela and Žakula, Jelena and Savić, Nada and Parac-Vogt, Tatjana and Krstić, Danijela Z.",
year = "2023",
abstract = "The objective of this study was to evaluate in vitro the antitumor properties of Fe(III)- substituted monolacunary Wells-Dawson polyoxotungstate, K7[FeIII(α2-P2W17O61)(H2O)] (FeWD) using cervical carcinoma HeLa cells as a model system. HeLa cells were exposed in vitro to FeWD within the concentration range from 0.001 to 1 mM, for 24, 48, and 72 hours. The studied Fe(III)- substituted polyoxotungstate affected HeLa cell viability in a concentration- and time-dependent manner. The obtained IC50 values (µM), as an indicator of the cytotoxic potential of FeWD, were: 16.64 ± 0.49, 10.75 ± 0.97, and 9.64 ± 0.19 for 24-, 48-, and 72-hour treatment, respectively. FeWD exhibited a stronger antitumor potential against HeLa cells than the structurally similar monolacunary Wells-Dawson polyoxotungstate, K10P2W17O61.20H2O (lacunary WD). Lacunary WD achieved IC50 at 24,11 µM after 24-hour exposure, which is about 44% higher concentration compared to the corresponding IC50 obtained for FeWD. This indicates that incorporating Fe(III) might be a new strategy for improving the antitumor efficacy of polyoxometalates as promising candidates for next-generation chemotherapeutics.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "The influence of Fe(III) incorporation on anti-cancer potential of a Wells-Dawson nanocluster",
pages = "419-422",
doi = "10.46793/ICCBI23.419C"
}

Čolović, M., Korićanac, L., Žakula, J., Savić, N., Parac-Vogt, T.,& Krstić, D. Z.. (2023). The influence of Fe(III) incorporation on anti-cancer potential of a Wells-Dawson nanocluster. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 419-422.
https://doi.org/10.46793/ICCBI23.419C

Čolović M, Korićanac L, Žakula J, Savić N, Parac-Vogt T, Krstić DZ. The influence of Fe(III) incorporation on anti-cancer potential of a Wells-Dawson nanocluster. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:419-422.
doi:10.46793/ICCBI23.419C .

Čolović, Mirjana, Korićanac, Lela, Žakula, Jelena, Savić, Nada, Parac-Vogt, Tatjana, Krstić, Danijela Z., "The influence of Fe(III) incorporation on anti-cancer potential of a Wells-Dawson nanocluster" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):419-422,
https://doi.org/10.46793/ICCBI23.419C . .

TY  - CONF
AU  - Parac-Vogt, Tatjana
AU  - Savić, Nada
AU  - Kerckhofs, Greet
AU  - Stojanović, Marko
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12940
AB  - Due to the high complexity and heterogeneous structure of biological tissues, imaging techniques that allow for precise and quantitative structural analyses of such materials are of high importance. These techniques could also advance clinical translation of regenerative medicine by providing better insights in tissue development and disease. The standard techniques for evaluating biological tissues such as histological sectioning and staining, have a high discriminative power, but allow assessment of the tissue distribution only in two dimensions. Due to restricted sectioning orientation and limited depth resolution, this leads to loss of information in three dimensions (3D), and therefore, more precise imaging of the 3D microstructure and spatial interrelationships of the different tissues within organs is crucial. MicroCT can provide full 3D structural information of mineralized tissues and dense biomaterials. However, the intrinsic low X-ray absorption of soft tissues requires contrast-enhancing staining agents (CESAs) to be used. We have shown that a range of polyoxometalate clusters (POMs) can be excellent non-destructive staining agents for high-resolution contrast-enhanced microCT (CE-CT) visualization of various tissues, of bone and its marrow vascularization and adiposity. A range of Wells-Dawson POMs, differing in structure and overall charge, has been synthesized and evaluated for their potential as soft tissue CESAs. We have shown that hafnium-substituted POM (Hf-POM) allows for simultaneous contrast-enhanced microCT (CE-CT) visualization of bone and its marrow vascularization and adiposity. Monolacunary Wells-Dawson POM (Mono-WD POM) showed similar soft tissue enhancement as Hf-WD POM and phosphotungstic acid (PTA), a frequently used but destructive CESA. However, compared to PTA, the POMs are much less destructive and show a better diffusion. The solubility of Mono-WD POM can be improved by simple addition of lithium chloride to the staining solution, leading to further enhancement of the soft tissue contrast. In vivo toxicity of Wells-Dawson POM has been also evaluated according to standard toxicological protocols using Wistar albino rats, which is the first and important step in evaluating side effects of these polyoxometalate nanoclusters that show large potential as therapeutics and contrast agents.
C3  - Medical Research : Medicinska istraživanja
T1  - Exploring polyoxometalates as non-destructive staining agents for contrast-enhanced computed tomography
VL  - 56
IS  - 4
SP  - 129
EP  - 129
DO  - 10.5937/medi56-47579
ER  - 

@conference{
author = "Parac-Vogt, Tatjana and Savić, Nada and Kerckhofs, Greet and Stojanović, Marko and Čolović, Mirjana and Krstić, Danijela",
year = "2023",
abstract = "Due to the high complexity and heterogeneous structure of biological tissues, imaging techniques that allow for precise and quantitative structural analyses of such materials are of high importance. These techniques could also advance clinical translation of regenerative medicine by providing better insights in tissue development and disease. The standard techniques for evaluating biological tissues such as histological sectioning and staining, have a high discriminative power, but allow assessment of the tissue distribution only in two dimensions. Due to restricted sectioning orientation and limited depth resolution, this leads to loss of information in three dimensions (3D), and therefore, more precise imaging of the 3D microstructure and spatial interrelationships of the different tissues within organs is crucial. MicroCT can provide full 3D structural information of mineralized tissues and dense biomaterials. However, the intrinsic low X-ray absorption of soft tissues requires contrast-enhancing staining agents (CESAs) to be used. We have shown that a range of polyoxometalate clusters (POMs) can be excellent non-destructive staining agents for high-resolution contrast-enhanced microCT (CE-CT) visualization of various tissues, of bone and its marrow vascularization and adiposity. A range of Wells-Dawson POMs, differing in structure and overall charge, has been synthesized and evaluated for their potential as soft tissue CESAs. We have shown that hafnium-substituted POM (Hf-POM) allows for simultaneous contrast-enhanced microCT (CE-CT) visualization of bone and its marrow vascularization and adiposity. Monolacunary Wells-Dawson POM (Mono-WD POM) showed similar soft tissue enhancement as Hf-WD POM and phosphotungstic acid (PTA), a frequently used but destructive CESA. However, compared to PTA, the POMs are much less destructive and show a better diffusion. The solubility of Mono-WD POM can be improved by simple addition of lithium chloride to the staining solution, leading to further enhancement of the soft tissue contrast. In vivo toxicity of Wells-Dawson POM has been also evaluated according to standard toxicological protocols using Wistar albino rats, which is the first and important step in evaluating side effects of these polyoxometalate nanoclusters that show large potential as therapeutics and contrast agents.",
journal = "Medical Research : Medicinska istraživanja",
title = "Exploring polyoxometalates as non-destructive staining agents for contrast-enhanced computed tomography",
volume = "56",
number = "4",
pages = "129-129",
doi = "10.5937/medi56-47579"
}

Parac-Vogt, T., Savić, N., Kerckhofs, G., Stojanović, M., Čolović, M.,& Krstić, D.. (2023). Exploring polyoxometalates as non-destructive staining agents for contrast-enhanced computed tomography. in Medical Research : Medicinska istraživanja, 56(4), 129-129.
https://doi.org/10.5937/medi56-47579

Parac-Vogt T, Savić N, Kerckhofs G, Stojanović M, Čolović M, Krstić D. Exploring polyoxometalates as non-destructive staining agents for contrast-enhanced computed tomography. in Medical Research : Medicinska istraživanja. 2023;56(4):129-129.
doi:10.5937/medi56-47579 .

Parac-Vogt, Tatjana, Savić, Nada, Kerckhofs, Greet, Stojanović, Marko, Čolović, Mirjana, Krstić, Danijela, "Exploring polyoxometalates as non-destructive staining agents for contrast-enhanced computed tomography" in Medical Research : Medicinska istraživanja, 56, no. 4 (2023):129-129,
https://doi.org/10.5937/medi56-47579 . .

TY  - JOUR
AU  - Stojanović, Marko
AU  - Lalatović, Jovana
AU  - Milosavljević, Aleksandra
AU  - Savić, Nada
AU  - Simms, Charlotte
AU  - Radosavljević, Branimir
AU  - Ćetković, Mila
AU  - Kravić Stevović, Tamara
AU  - Mrda, Davor
AU  - Čolović, Mirjana B.
AU  - Parac-Vogt, Tatjana N.
AU  - Krstić, Danijela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11091
AB  - In this study, we demonstrate for the first time, that a discrete metal-oxo cluster α-/β-K 6 P 2 W 18 O 62 (WD-POM) exhibits superior performance as a computed tomography (CT) contrast agent, in comparison to the standard contrast agent iohexol. A toxicity evaluation of WD-POM was performed according to standard toxicological protocols using Wistar albino rats. The maximum tolerable dose (MTD) of 2000 mg/kg was initially determined after oral WD-POM application. The acute intravenous toxicity of single WD-POM doses (1/3, 1/5, and 1/10 MTD), which are at least fifty times higher than the typically used dose (0.015 mmol W kg −1 ) of tungsten-based contrast agents, was evaluated for 14 days. The results of arterial blood gas analysis, CO-oximetry status, electrolyte and lactate levels for 1/10 MTD group (80% survival rate) indicated the mixed respiratory and metabolic acidosis. The highest deposition of WD-POM (0.6 ppm tungsten) was found in the kidney, followed by liver (0.15 ppm tungsten), for which the histological analysis revealed morphological irregularities, although the renal function parameters (creatinine and BUN levels) were within the physiological range. This study is the first and important step in evaluating side effects of polyoxometalate nanoclusters, which in recent years have shown a large potential as therapeutics and contrast agents.
T2  - Scientific Reports
T1  - In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography
VL  - 13
IS  - 1
SP  - 9140
DO  - 10.1038/s41598-023-36317-8
ER  - 

@article{
author = "Stojanović, Marko and Lalatović, Jovana and Milosavljević, Aleksandra and Savić, Nada and Simms, Charlotte and Radosavljević, Branimir and Ćetković, Mila and Kravić Stevović, Tamara and Mrda, Davor and Čolović, Mirjana B. and Parac-Vogt, Tatjana N. and Krstić, Danijela",
year = "2023",
abstract = "In this study, we demonstrate for the first time, that a discrete metal-oxo cluster α-/β-K 6 P 2 W 18 O 62 (WD-POM) exhibits superior performance as a computed tomography (CT) contrast agent, in comparison to the standard contrast agent iohexol. A toxicity evaluation of WD-POM was performed according to standard toxicological protocols using Wistar albino rats. The maximum tolerable dose (MTD) of 2000 mg/kg was initially determined after oral WD-POM application. The acute intravenous toxicity of single WD-POM doses (1/3, 1/5, and 1/10 MTD), which are at least fifty times higher than the typically used dose (0.015 mmol W kg −1 ) of tungsten-based contrast agents, was evaluated for 14 days. The results of arterial blood gas analysis, CO-oximetry status, electrolyte and lactate levels for 1/10 MTD group (80% survival rate) indicated the mixed respiratory and metabolic acidosis. The highest deposition of WD-POM (0.6 ppm tungsten) was found in the kidney, followed by liver (0.15 ppm tungsten), for which the histological analysis revealed morphological irregularities, although the renal function parameters (creatinine and BUN levels) were within the physiological range. This study is the first and important step in evaluating side effects of polyoxometalate nanoclusters, which in recent years have shown a large potential as therapeutics and contrast agents.",
journal = "Scientific Reports",
title = "In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography",
volume = "13",
number = "1",
pages = "9140",
doi = "10.1038/s41598-023-36317-8"
}

Stojanović, M., Lalatović, J., Milosavljević, A., Savić, N., Simms, C., Radosavljević, B., Ćetković, M., Kravić Stevović, T., Mrda, D., Čolović, M. B., Parac-Vogt, T. N.,& Krstić, D.. (2023). In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography. in Scientific Reports, 13(1), 9140.
https://doi.org/10.1038/s41598-023-36317-8

Stojanović M, Lalatović J, Milosavljević A, Savić N, Simms C, Radosavljević B, Ćetković M, Kravić Stevović T, Mrda D, Čolović MB, Parac-Vogt TN, Krstić D. In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography. in Scientific Reports. 2023;13(1):9140.
doi:10.1038/s41598-023-36317-8 .

Stojanović, Marko, Lalatović, Jovana, Milosavljević, Aleksandra, Savić, Nada, Simms, Charlotte, Radosavljević, Branimir, Ćetković, Mila, Kravić Stevović, Tamara, Mrda, Davor, Čolović, Mirjana B., Parac-Vogt, Tatjana N., Krstić, Danijela, "In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography" in Scientific Reports, 13, no. 1 (2023):9140,
https://doi.org/10.1038/s41598-023-36317-8 . .

TY  - CONF
AU  - Čolović, Mirjana
AU  - Gajski, Goran
AU  - Domijan, Ana-Marija
AU  - Gerić, Marko
AU  - Savić, Nada
AU  - Parac-Vogt, Tatjana
AU  - Krstić, Danijela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12229
AB  - Tungsten-based nanoparticles possess high density and capability to attenuate X-rays and thus have been studied as interesting candidates for the development of new-generation contrast-enhancing staining agents (CESAs) for computed tomography (CT). Polyoxotungstates, as polyoxoanions containing tungsten in its high oxidation state (W6+) were reported as promising CESA candidates to visualize long bones and kidney tissues. However, some polyoxotungstates induced side effects in toxicity studies in vivo, which could limit their clinical application. Thus, the aim of this study was to evaluate genotoxic effects in vitro of monolacunary Wells-Dawson polyoxotungstate, α2-K10P2W17O61.20H2O (lacunary WD) that was found as a potential contrast agent for CT in our previous studies in situ and in vitro. Lacunary WD was synthesized by following the reported procedure. The genotoxicity evaluation was performed by using the standard procedure for the alkaline comet assay. Briefly, human whole blood samples were taken from healthy male and female donors and exposed to different lacunary WD concentrations within the range of 10-6-10-4 mol/L, for 4 and 24 h at 37 °C. Then, 5 μL of whole blood was embedded into an agarose matrix and subsequently lysed (2.5 M NaCl, 100 mM EDTANa2, 10 mM Tris, 1% sodium sarcosinate, 1% Triton X-100, 10% DMSO, pH 10) overnight at 4 °C. After the lysis, the slides were placed into an alkaline solution (300 mM NaOH, 1 mM EDTANa2, pH 13) for 20 min at 4 °C to allow DNA unwinding and subsequently electrophoresed for 20 min at 1 V/cm. Finally, the slides were neutralized in 0.4 M Tris buffer (pH 7.5) for 5 min 3 times, stained with ethidium bromide (10 μg/mL), and analyzed at 250× magnification using an epifluorescence microscope (Zeiss, Göttingen, Germany) connected through a camera to an image analysis system (Comet Assay II; Perceptive Instruments Ltd., Haverhill, Suffolk, UK). One hundred randomly captured comets from each slide were examined. Multiple comparisons between groups were done by means of ANOVA on log-transformed data. Post hoc analyses of the differences were done by the Scheffé test. The percentage of tail DNA was determined to evaluate the level of DNA damage and genotoxicity potential. The obtained results showed that lacunary WD did not induce a statistically significant relative increase of tail DNA compared to the corresponding control at all investigated concentrations, after both 4 and 24 h exposure. Accordingly, the investigated promising contrast agent candidate could be regarded in further studies as toxicologically safe for healthy human blood cells from a genotoxicity point of view.
PB  - Niš : RAD Centre
C3  - RAD 2023 : 11th International Conference on Radiation Natural Sciences, Medicine, Engineering, Technology and Ecology : Book of Abstracts
T1  - In vitro genotoxicity assessment of a monolacunary Wells-Dawson nanocluster as a promising contrast agent candidate
SP  - 135
EP  - 135
DO  - 10.21175/rad.abstr.book.2023.23.3
ER  - 

@conference{
author = "Čolović, Mirjana and Gajski, Goran and Domijan, Ana-Marija and Gerić, Marko and Savić, Nada and Parac-Vogt, Tatjana and Krstić, Danijela",
year = "2023",
abstract = "Tungsten-based nanoparticles possess high density and capability to attenuate X-rays and thus have been studied as interesting candidates for the development of new-generation contrast-enhancing staining agents (CESAs) for computed tomography (CT). Polyoxotungstates, as polyoxoanions containing tungsten in its high oxidation state (W6+) were reported as promising CESA candidates to visualize long bones and kidney tissues. However, some polyoxotungstates induced side effects in toxicity studies in vivo, which could limit their clinical application. Thus, the aim of this study was to evaluate genotoxic effects in vitro of monolacunary Wells-Dawson polyoxotungstate, α2-K10P2W17O61.20H2O (lacunary WD) that was found as a potential contrast agent for CT in our previous studies in situ and in vitro. Lacunary WD was synthesized by following the reported procedure. The genotoxicity evaluation was performed by using the standard procedure for the alkaline comet assay. Briefly, human whole blood samples were taken from healthy male and female donors and exposed to different lacunary WD concentrations within the range of 10-6-10-4 mol/L, for 4 and 24 h at 37 °C. Then, 5 μL of whole blood was embedded into an agarose matrix and subsequently lysed (2.5 M NaCl, 100 mM EDTANa2, 10 mM Tris, 1% sodium sarcosinate, 1% Triton X-100, 10% DMSO, pH 10) overnight at 4 °C. After the lysis, the slides were placed into an alkaline solution (300 mM NaOH, 1 mM EDTANa2, pH 13) for 20 min at 4 °C to allow DNA unwinding and subsequently electrophoresed for 20 min at 1 V/cm. Finally, the slides were neutralized in 0.4 M Tris buffer (pH 7.5) for 5 min 3 times, stained with ethidium bromide (10 μg/mL), and analyzed at 250× magnification using an epifluorescence microscope (Zeiss, Göttingen, Germany) connected through a camera to an image analysis system (Comet Assay II; Perceptive Instruments Ltd., Haverhill, Suffolk, UK). One hundred randomly captured comets from each slide were examined. Multiple comparisons between groups were done by means of ANOVA on log-transformed data. Post hoc analyses of the differences were done by the Scheffé test. The percentage of tail DNA was determined to evaluate the level of DNA damage and genotoxicity potential. The obtained results showed that lacunary WD did not induce a statistically significant relative increase of tail DNA compared to the corresponding control at all investigated concentrations, after both 4 and 24 h exposure. Accordingly, the investigated promising contrast agent candidate could be regarded in further studies as toxicologically safe for healthy human blood cells from a genotoxicity point of view.",
publisher = "Niš : RAD Centre",
journal = "RAD 2023 : 11th International Conference on Radiation Natural Sciences, Medicine, Engineering, Technology and Ecology : Book of Abstracts",
title = "In vitro genotoxicity assessment of a monolacunary Wells-Dawson nanocluster as a promising contrast agent candidate",
pages = "135-135",
doi = "10.21175/rad.abstr.book.2023.23.3"
}

Čolović, M., Gajski, G., Domijan, A., Gerić, M., Savić, N., Parac-Vogt, T.,& Krstić, D.. (2023). In vitro genotoxicity assessment of a monolacunary Wells-Dawson nanocluster as a promising contrast agent candidate. in RAD 2023 : 11th International Conference on Radiation Natural Sciences, Medicine, Engineering, Technology and Ecology : Book of Abstracts
Niš : RAD Centre., 135-135.
https://doi.org/10.21175/rad.abstr.book.2023.23.3

Čolović M, Gajski G, Domijan A, Gerić M, Savić N, Parac-Vogt T, Krstić D. In vitro genotoxicity assessment of a monolacunary Wells-Dawson nanocluster as a promising contrast agent candidate. in RAD 2023 : 11th International Conference on Radiation Natural Sciences, Medicine, Engineering, Technology and Ecology : Book of Abstracts. 2023;:135-135.
doi:10.21175/rad.abstr.book.2023.23.3 .

Čolović, Mirjana, Gajski, Goran, Domijan, Ana-Marija, Gerić, Marko, Savić, Nada, Parac-Vogt, Tatjana, Krstić, Danijela, "In vitro genotoxicity assessment of a monolacunary Wells-Dawson nanocluster as a promising contrast agent candidate" in RAD 2023 : 11th International Conference on Radiation Natural Sciences, Medicine, Engineering, Technology and Ecology : Book of Abstracts (2023):135-135,
https://doi.org/10.21175/rad.abstr.book.2023.23.3 . .

TY  - JOUR
AU  - Pavić, Aleksandar B.
AU  - Savić, Nada D.
AU  - Glišić, Biljana Đ.
AU  - Crochet, Aurélien
AU  - Vojnović, Sandra
AU  - Kurutos, Atanas
AU  - Stanković, Dalibor M.
AU  - Fromm, Katharina M.
AU  - Nikodinović-Runić, Jasmina
AU  - Đuran, Miloš I.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8127
AB  - Five novel silver(I) complexes with 4,7-phenanthroline (4,7-phen), [Ag(NO 3 -O)(4,7-phen-μ-N4,N7)] n (1), [Ag(ClO 4 -О)(4,7-phen-μ-N4,N7)] n (2), [Ag(CF 3 COO-O)(4,7-phen-μ-N4,N7)] n (3), [Ag 2 (H 2 O) 0.58 (4,7-phen) 3 ](SbF 6 ) 2 (4) and {[Ag 2 (H 2 O)(4,7-phen-μ-N4,N7) 2 ](BF 4 ) 2 } n (5) were synthesized, structurally elucidated and biologically evaluated. These complexes showed selectivity towards Candida spp. in comparison to the tested bacteria and effectively inhibited the growth of four different Candida species, particularly of C. albicans strains, with minimal inhibitory concentrations (MICs) in the range of 2.0–10.0 μM. In order to evaluate the therapeutic potential of 1–5, in vivo toxicity studies were conducted in the zebrafish model. Based on the favorable therapeutic profiles, complexes 1, 3 and 5 were selected for the evaluation of their antifungal efficacy in vivo using the zebrafish model of lethal disseminated candidiasis. Complexes 1 and 3 efficiently controlled and prevented fungal filamentation even at sub-MIC doses, while drastically increased the survival of the infected embryos. Moreover, at the MIC doses, both complexes totally prevented C. albicans filamentation and rescued almost all infected fish of the fatal infection outcome. On the other side, complex 5, which demonstrated the highest antifungal activity in vitro, affected the neutrophils occurrence of the infected host, failed to inhibit the C. albicans cells filamentation and showed a poor potential to cure candidal infection, highlighting the importance of the in vivo activity evaluation early in the therapeutic design and development process. The mechanism of action of the investigated silver(I) complexes was related to the induction of reactive oxygen species (ROS) response in C. albicans, with DNA being one of the possible target biomolecules. © 2019 Elsevier Inc.
T2  - Journal of Inorganic Biochemistry
T1  - Silver(I) complexes with 4,7-phenanthroline efficient in rescuing the zebrafish embryos of lethal Candida albicans infection
VL  - 195
SP  - 149
EP  - 163
DO  - 10.1016/j.jinorgbio.2019.03.017
ER  - 

@article{
author = "Pavić, Aleksandar B. and Savić, Nada D. and Glišić, Biljana Đ. and Crochet, Aurélien and Vojnović, Sandra and Kurutos, Atanas and Stanković, Dalibor M. and Fromm, Katharina M. and Nikodinović-Runić, Jasmina and Đuran, Miloš I.",
year = "2019",
abstract = "Five novel silver(I) complexes with 4,7-phenanthroline (4,7-phen), [Ag(NO 3 -O)(4,7-phen-μ-N4,N7)] n (1), [Ag(ClO 4 -О)(4,7-phen-μ-N4,N7)] n (2), [Ag(CF 3 COO-O)(4,7-phen-μ-N4,N7)] n (3), [Ag 2 (H 2 O) 0.58 (4,7-phen) 3 ](SbF 6 ) 2 (4) and {[Ag 2 (H 2 O)(4,7-phen-μ-N4,N7) 2 ](BF 4 ) 2 } n (5) were synthesized, structurally elucidated and biologically evaluated. These complexes showed selectivity towards Candida spp. in comparison to the tested bacteria and effectively inhibited the growth of four different Candida species, particularly of C. albicans strains, with minimal inhibitory concentrations (MICs) in the range of 2.0–10.0 μM. In order to evaluate the therapeutic potential of 1–5, in vivo toxicity studies were conducted in the zebrafish model. Based on the favorable therapeutic profiles, complexes 1, 3 and 5 were selected for the evaluation of their antifungal efficacy in vivo using the zebrafish model of lethal disseminated candidiasis. Complexes 1 and 3 efficiently controlled and prevented fungal filamentation even at sub-MIC doses, while drastically increased the survival of the infected embryos. Moreover, at the MIC doses, both complexes totally prevented C. albicans filamentation and rescued almost all infected fish of the fatal infection outcome. On the other side, complex 5, which demonstrated the highest antifungal activity in vitro, affected the neutrophils occurrence of the infected host, failed to inhibit the C. albicans cells filamentation and showed a poor potential to cure candidal infection, highlighting the importance of the in vivo activity evaluation early in the therapeutic design and development process. The mechanism of action of the investigated silver(I) complexes was related to the induction of reactive oxygen species (ROS) response in C. albicans, with DNA being one of the possible target biomolecules. © 2019 Elsevier Inc.",
journal = "Journal of Inorganic Biochemistry",
title = "Silver(I) complexes with 4,7-phenanthroline efficient in rescuing the zebrafish embryos of lethal Candida albicans infection",
volume = "195",
pages = "149-163",
doi = "10.1016/j.jinorgbio.2019.03.017"
}

Pavić, A. B., Savić, N. D., Glišić, B. Đ., Crochet, A., Vojnović, S., Kurutos, A., Stanković, D. M., Fromm, K. M., Nikodinović-Runić, J.,& Đuran, M. I.. (2019). Silver(I) complexes with 4,7-phenanthroline efficient in rescuing the zebrafish embryos of lethal Candida albicans infection. in Journal of Inorganic Biochemistry, 195, 149-163.
https://doi.org/10.1016/j.jinorgbio.2019.03.017

Pavić AB, Savić ND, Glišić BĐ, Crochet A, Vojnović S, Kurutos A, Stanković DM, Fromm KM, Nikodinović-Runić J, Đuran MI. Silver(I) complexes with 4,7-phenanthroline efficient in rescuing the zebrafish embryos of lethal Candida albicans infection. in Journal of Inorganic Biochemistry. 2019;195:149-163.
doi:10.1016/j.jinorgbio.2019.03.017 .

Pavić, Aleksandar B., Savić, Nada D., Glišić, Biljana Đ., Crochet, Aurélien, Vojnović, Sandra, Kurutos, Atanas, Stanković, Dalibor M., Fromm, Katharina M., Nikodinović-Runić, Jasmina, Đuran, Miloš I., "Silver(I) complexes with 4,7-phenanthroline efficient in rescuing the zebrafish embryos of lethal Candida albicans infection" in Journal of Inorganic Biochemistry, 195 (2019):149-163,
https://doi.org/10.1016/j.jinorgbio.2019.03.017 . .

TY  - DATA
AU  - Pavić, Aleksandar B.
AU  - Savić, Nada D.
AU  - Glišić, Biljana Đ.
AU  - Crochet, Aurélien
AU  - Vojnović, Sandra
AU  - Kurutos, Atanas
AU  - Stanković, Dalibor M.
AU  - Fromm, Katharina M.
AU  - Nikodinović-Runić, Jasmina
AU  - Đuran, Miloš I.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8130
AB  - Data associated with the article "Pavic, A., Savić, N. D., Glišić, B. Đ., Crochet, A., Vojnovic, S., Kurutos, A., ... & Djuran, M. I. (2019). Silver (I) complexes with 4, 7-phenanthroline efficient in rescuing the zebrafish embryos of lethal Candida albicans infection. Journal of Inorganic Biochemistry."
T2  - The Cambridge Crystallographic Data Centre (CCDC)
T1  - CCDC 1879001: Experimental Crystal Structure Determination
DO  - 10.5517/ccdc.csd.cc2127yb
ER  - 

@misc{
author = "Pavić, Aleksandar B. and Savić, Nada D. and Glišić, Biljana Đ. and Crochet, Aurélien and Vojnović, Sandra and Kurutos, Atanas and Stanković, Dalibor M. and Fromm, Katharina M. and Nikodinović-Runić, Jasmina and Đuran, Miloš I.",
year = "2019",
abstract = "Data associated with the article "Pavic, A., Savić, N. D., Glišić, B. Đ., Crochet, A., Vojnovic, S., Kurutos, A., ... & Djuran, M. I. (2019). Silver (I) complexes with 4, 7-phenanthroline efficient in rescuing the zebrafish embryos of lethal Candida albicans infection. Journal of Inorganic Biochemistry."",
journal = "The Cambridge Crystallographic Data Centre (CCDC)",
title = "CCDC 1879001: Experimental Crystal Structure Determination",
doi = "10.5517/ccdc.csd.cc2127yb"
}

Pavić, A. B., Savić, N. D., Glišić, B. Đ., Crochet, A., Vojnović, S., Kurutos, A., Stanković, D. M., Fromm, K. M., Nikodinović-Runić, J.,& Đuran, M. I.. (2019). CCDC 1879001: Experimental Crystal Structure Determination. in The Cambridge Crystallographic Data Centre (CCDC).
https://doi.org/10.5517/ccdc.csd.cc2127yb

Pavić AB, Savić ND, Glišić BĐ, Crochet A, Vojnović S, Kurutos A, Stanković DM, Fromm KM, Nikodinović-Runić J, Đuran MI. CCDC 1879001: Experimental Crystal Structure Determination. in The Cambridge Crystallographic Data Centre (CCDC). 2019;.
doi:10.5517/ccdc.csd.cc2127yb .

Pavić, Aleksandar B., Savić, Nada D., Glišić, Biljana Đ., Crochet, Aurélien, Vojnović, Sandra, Kurutos, Atanas, Stanković, Dalibor M., Fromm, Katharina M., Nikodinović-Runić, Jasmina, Đuran, Miloš I., "CCDC 1879001: Experimental Crystal Structure Determination" in The Cambridge Crystallographic Data Centre (CCDC) (2019),
https://doi.org/10.5517/ccdc.csd.cc2127yb . .