TY  - JOUR
AU  - Pavić, Aleksandar B.
AU  - Savić, Nada D.
AU  - Glišić, Biljana Đ.
AU  - Crochet, Aurélien
AU  - Vojnović, Sandra
AU  - Kurutos, Atanas
AU  - Stanković, Dalibor M.
AU  - Fromm, Katharina M.
AU  - Nikodinović-Runić, Jasmina
AU  - Đuran, Miloš I.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8127
AB  - Five novel silver(I) complexes with 4,7-phenanthroline (4,7-phen), [Ag(NO 3 -O)(4,7-phen-μ-N4,N7)] n (1), [Ag(ClO 4 -О)(4,7-phen-μ-N4,N7)] n (2), [Ag(CF 3 COO-O)(4,7-phen-μ-N4,N7)] n (3), [Ag 2 (H 2 O) 0.58 (4,7-phen) 3 ](SbF 6 ) 2 (4) and {[Ag 2 (H 2 O)(4,7-phen-μ-N4,N7) 2 ](BF 4 ) 2 } n (5) were synthesized, structurally elucidated and biologically evaluated. These complexes showed selectivity towards Candida spp. in comparison to the tested bacteria and effectively inhibited the growth of four different Candida species, particularly of C. albicans strains, with minimal inhibitory concentrations (MICs) in the range of 2.0–10.0 μM. In order to evaluate the therapeutic potential of 1–5, in vivo toxicity studies were conducted in the zebrafish model. Based on the favorable therapeutic profiles, complexes 1, 3 and 5 were selected for the evaluation of their antifungal efficacy in vivo using the zebrafish model of lethal disseminated candidiasis. Complexes 1 and 3 efficiently controlled and prevented fungal filamentation even at sub-MIC doses, while drastically increased the survival of the infected embryos. Moreover, at the MIC doses, both complexes totally prevented C. albicans filamentation and rescued almost all infected fish of the fatal infection outcome. On the other side, complex 5, which demonstrated the highest antifungal activity in vitro, affected the neutrophils occurrence of the infected host, failed to inhibit the C. albicans cells filamentation and showed a poor potential to cure candidal infection, highlighting the importance of the in vivo activity evaluation early in the therapeutic design and development process. The mechanism of action of the investigated silver(I) complexes was related to the induction of reactive oxygen species (ROS) response in C. albicans, with DNA being one of the possible target biomolecules. © 2019 Elsevier Inc.
T2  - Journal of Inorganic Biochemistry
T1  - Silver(I) complexes with 4,7-phenanthroline efficient in rescuing the zebrafish embryos of lethal Candida albicans infection
VL  - 195
SP  - 149
EP  - 163
DO  - 10.1016/j.jinorgbio.2019.03.017
ER  - 

@article{
author = "Pavić, Aleksandar B. and Savić, Nada D. and Glišić, Biljana Đ. and Crochet, Aurélien and Vojnović, Sandra and Kurutos, Atanas and Stanković, Dalibor M. and Fromm, Katharina M. and Nikodinović-Runić, Jasmina and Đuran, Miloš I.",
year = "2019",
abstract = "Five novel silver(I) complexes with 4,7-phenanthroline (4,7-phen), [Ag(NO 3 -O)(4,7-phen-μ-N4,N7)] n (1), [Ag(ClO 4 -О)(4,7-phen-μ-N4,N7)] n (2), [Ag(CF 3 COO-O)(4,7-phen-μ-N4,N7)] n (3), [Ag 2 (H 2 O) 0.58 (4,7-phen) 3 ](SbF 6 ) 2 (4) and {[Ag 2 (H 2 O)(4,7-phen-μ-N4,N7) 2 ](BF 4 ) 2 } n (5) were synthesized, structurally elucidated and biologically evaluated. These complexes showed selectivity towards Candida spp. in comparison to the tested bacteria and effectively inhibited the growth of four different Candida species, particularly of C. albicans strains, with minimal inhibitory concentrations (MICs) in the range of 2.0–10.0 μM. In order to evaluate the therapeutic potential of 1–5, in vivo toxicity studies were conducted in the zebrafish model. Based on the favorable therapeutic profiles, complexes 1, 3 and 5 were selected for the evaluation of their antifungal efficacy in vivo using the zebrafish model of lethal disseminated candidiasis. Complexes 1 and 3 efficiently controlled and prevented fungal filamentation even at sub-MIC doses, while drastically increased the survival of the infected embryos. Moreover, at the MIC doses, both complexes totally prevented C. albicans filamentation and rescued almost all infected fish of the fatal infection outcome. On the other side, complex 5, which demonstrated the highest antifungal activity in vitro, affected the neutrophils occurrence of the infected host, failed to inhibit the C. albicans cells filamentation and showed a poor potential to cure candidal infection, highlighting the importance of the in vivo activity evaluation early in the therapeutic design and development process. The mechanism of action of the investigated silver(I) complexes was related to the induction of reactive oxygen species (ROS) response in C. albicans, with DNA being one of the possible target biomolecules. © 2019 Elsevier Inc.",
journal = "Journal of Inorganic Biochemistry",
title = "Silver(I) complexes with 4,7-phenanthroline efficient in rescuing the zebrafish embryos of lethal Candida albicans infection",
volume = "195",
pages = "149-163",
doi = "10.1016/j.jinorgbio.2019.03.017"
}

Pavić, A. B., Savić, N. D., Glišić, B. Đ., Crochet, A., Vojnović, S., Kurutos, A., Stanković, D. M., Fromm, K. M., Nikodinović-Runić, J.,& Đuran, M. I.. (2019). Silver(I) complexes with 4,7-phenanthroline efficient in rescuing the zebrafish embryos of lethal Candida albicans infection. in Journal of Inorganic Biochemistry, 195, 149-163.
https://doi.org/10.1016/j.jinorgbio.2019.03.017

Pavić AB, Savić ND, Glišić BĐ, Crochet A, Vojnović S, Kurutos A, Stanković DM, Fromm KM, Nikodinović-Runić J, Đuran MI. Silver(I) complexes with 4,7-phenanthroline efficient in rescuing the zebrafish embryos of lethal Candida albicans infection. in Journal of Inorganic Biochemistry. 2019;195:149-163.
doi:10.1016/j.jinorgbio.2019.03.017 .

Pavić, Aleksandar B., Savić, Nada D., Glišić, Biljana Đ., Crochet, Aurélien, Vojnović, Sandra, Kurutos, Atanas, Stanković, Dalibor M., Fromm, Katharina M., Nikodinović-Runić, Jasmina, Đuran, Miloš I., "Silver(I) complexes with 4,7-phenanthroline efficient in rescuing the zebrafish embryos of lethal Candida albicans infection" in Journal of Inorganic Biochemistry, 195 (2019):149-163,
https://doi.org/10.1016/j.jinorgbio.2019.03.017 . .

TY  - DATA
AU  - Pavić, Aleksandar B.
AU  - Savić, Nada D.
AU  - Glišić, Biljana Đ.
AU  - Crochet, Aurélien
AU  - Vojnović, Sandra
AU  - Kurutos, Atanas
AU  - Stanković, Dalibor M.
AU  - Fromm, Katharina M.
AU  - Nikodinović-Runić, Jasmina
AU  - Đuran, Miloš I.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8130
AB  - Data associated with the article "Pavic, A., Savić, N. D., Glišić, B. Đ., Crochet, A., Vojnovic, S., Kurutos, A., ... & Djuran, M. I. (2019). Silver (I) complexes with 4, 7-phenanthroline efficient in rescuing the zebrafish embryos of lethal Candida albicans infection. Journal of Inorganic Biochemistry."
T2  - The Cambridge Crystallographic Data Centre (CCDC)
T1  - CCDC 1879001: Experimental Crystal Structure Determination
DO  - 10.5517/ccdc.csd.cc2127yb
ER  - 

@misc{
author = "Pavić, Aleksandar B. and Savić, Nada D. and Glišić, Biljana Đ. and Crochet, Aurélien and Vojnović, Sandra and Kurutos, Atanas and Stanković, Dalibor M. and Fromm, Katharina M. and Nikodinović-Runić, Jasmina and Đuran, Miloš I.",
year = "2019",
abstract = "Data associated with the article "Pavic, A., Savić, N. D., Glišić, B. Đ., Crochet, A., Vojnovic, S., Kurutos, A., ... & Djuran, M. I. (2019). Silver (I) complexes with 4, 7-phenanthroline efficient in rescuing the zebrafish embryos of lethal Candida albicans infection. Journal of Inorganic Biochemistry."",
journal = "The Cambridge Crystallographic Data Centre (CCDC)",
title = "CCDC 1879001: Experimental Crystal Structure Determination",
doi = "10.5517/ccdc.csd.cc2127yb"
}

Pavić, A. B., Savić, N. D., Glišić, B. Đ., Crochet, A., Vojnović, S., Kurutos, A., Stanković, D. M., Fromm, K. M., Nikodinović-Runić, J.,& Đuran, M. I.. (2019). CCDC 1879001: Experimental Crystal Structure Determination. in The Cambridge Crystallographic Data Centre (CCDC).
https://doi.org/10.5517/ccdc.csd.cc2127yb

Pavić AB, Savić ND, Glišić BĐ, Crochet A, Vojnović S, Kurutos A, Stanković DM, Fromm KM, Nikodinović-Runić J, Đuran MI. CCDC 1879001: Experimental Crystal Structure Determination. in The Cambridge Crystallographic Data Centre (CCDC). 2019;.
doi:10.5517/ccdc.csd.cc2127yb .

Pavić, Aleksandar B., Savić, Nada D., Glišić, Biljana Đ., Crochet, Aurélien, Vojnović, Sandra, Kurutos, Atanas, Stanković, Dalibor M., Fromm, Katharina M., Nikodinović-Runić, Jasmina, Đuran, Miloš I., "CCDC 1879001: Experimental Crystal Structure Determination" in The Cambridge Crystallographic Data Centre (CCDC) (2019),
https://doi.org/10.5517/ccdc.csd.cc2127yb . .

TY  - JOUR
AU  - Milivojević, Dušan
AU  - Šumonja, Neven
AU  - Medić, Strahinja
AU  - Pavić, Aleksandar B.
AU  - Morić, Ivana
AU  - Vasiljević, Branka
AU  - Senerović, Lidija
AU  - Nikodinović-Runić, Jasmina
PY  - 2018
UR  - https://academic.oup.com/femspd/article/doi/10.1093/femspd/fty041/4978417
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7822
AB  - Pseudomonas aeruginosa has been amongst the top 10 'superbugs' worldwide and is causing infections with poor outcomes in both humans and animals. From 202 P. aeruginosa isolates (n = 121 animal and n = 81 human), 40 were selected on the basis of biofilm-forming ability and were comparatively characterized in terms of virulence determinants to the type strain P. aeruginosa PAO1. Biofilm formation, pyocyanin and hemolysin production, and bacterial motility patterns were compared with the ability to kill human cell line A549 in vitro. On average, there was no significant difference between levels of animal and human cytotoxicity, while human isolates produced higher amounts of pyocyanin, hemolysins and showed increased swimming ability. Non-parametric statistical analysis identified the highest positive correlation between hemolysis and the swarming ability. For the first time an ensemble machine learning approach used on the in vitro virulence data determined the highest relative predictive importance of the submerged biofilm formation for the cytotoxicity, as an indicator of the infection ability. The findings from the in vitro study were validated in vivo using zebrafish (Danio rerio) embryos. This study highlighted no major differences between P. aeruginosa species isolated from animal and human infections and the importance of pyocyanin production in cytotoxicity and infection ability. © FEMS 2018.
T2  - Pathogens and Disease
T1  - Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans
VL  - 76
IS  - 4
SP  - fty041
DO  - 10.1093/femspd/fty041
ER  - 

@article{
author = "Milivojević, Dušan and Šumonja, Neven and Medić, Strahinja and Pavić, Aleksandar B. and Morić, Ivana and Vasiljević, Branka and Senerović, Lidija and Nikodinović-Runić, Jasmina",
year = "2018",
abstract = "Pseudomonas aeruginosa has been amongst the top 10 'superbugs' worldwide and is causing infections with poor outcomes in both humans and animals. From 202 P. aeruginosa isolates (n = 121 animal and n = 81 human), 40 were selected on the basis of biofilm-forming ability and were comparatively characterized in terms of virulence determinants to the type strain P. aeruginosa PAO1. Biofilm formation, pyocyanin and hemolysin production, and bacterial motility patterns were compared with the ability to kill human cell line A549 in vitro. On average, there was no significant difference between levels of animal and human cytotoxicity, while human isolates produced higher amounts of pyocyanin, hemolysins and showed increased swimming ability. Non-parametric statistical analysis identified the highest positive correlation between hemolysis and the swarming ability. For the first time an ensemble machine learning approach used on the in vitro virulence data determined the highest relative predictive importance of the submerged biofilm formation for the cytotoxicity, as an indicator of the infection ability. The findings from the in vitro study were validated in vivo using zebrafish (Danio rerio) embryos. This study highlighted no major differences between P. aeruginosa species isolated from animal and human infections and the importance of pyocyanin production in cytotoxicity and infection ability. © FEMS 2018.",
journal = "Pathogens and Disease",
title = "Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans",
volume = "76",
number = "4",
pages = "fty041",
doi = "10.1093/femspd/fty041"
}

Milivojević, D., Šumonja, N., Medić, S., Pavić, A. B., Morić, I., Vasiljević, B., Senerović, L.,& Nikodinović-Runić, J.. (2018). Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans. in Pathogens and Disease, 76(4), fty041.
https://doi.org/10.1093/femspd/fty041

Milivojević D, Šumonja N, Medić S, Pavić AB, Morić I, Vasiljević B, Senerović L, Nikodinović-Runić J. Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans. in Pathogens and Disease. 2018;76(4):fty041.
doi:10.1093/femspd/fty041 .

Milivojević, Dušan, Šumonja, Neven, Medić, Strahinja, Pavić, Aleksandar B., Morić, Ivana, Vasiljević, Branka, Senerović, Lidija, Nikodinović-Runić, Jasmina, "Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans" in Pathogens and Disease, 76, no. 4 (2018):fty041,
https://doi.org/10.1093/femspd/fty041 . .

TY  - JOUR
AU  - Jović, Danica S.
AU  - Seke, Mariana
AU  - Đorđević, Aleksandar N.
AU  - Mrđanović, Jasminka Ž.
AU  - Aleksić, Lidija D.
AU  - Bogdanović, Gordana M.
AU  - Pavić, Aleksandar B.
AU  - Plavec, Janez
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1042
AB  - Doxorubicin is a very potent chemotherapeutic drug, however its side effects limit its clinical use. The aim of this research was to investigate the properties of a fullerenol/doxorubicin nanocomposite, its potentially cytotoxic and genotoxic effects on malignant cell lines, as well as its toxicity towards zebra fish embryos. Chromatographic, NMR and mass spectral analysis of the nanocomposite imply that interactions between doxorubicin and fullerenol are non-covalent bonds. The stability of the nanocomposite was confirmed by the use of atomic force microscopy, dynamic light scattering and transmission electron microscopy. The nanocomposite, compared to the free doxorubicin at equivalent concentrations, significantly decreased the viability of MCF-7 and MDA-MB-231 cells. The flow cytometry results indicated that doxorubicin-loaded fullerenol could remarkably increase the uptake of doxorubicin suggesting that fullerenol might be a promising intracellular targeting carrier for the efficient delivery of antitumor drugs into tumor cells. The nanocomposite also affected cell cycle distribution. A genotoxicity test showed that the nanocomposite at all examined concentrations on MCF-7 and at lower concentrations on MDA-MB-231 cells caused DNA damage. Consequently, cell proliferation was notably reduced when compared with controls. Results of the zebrafish embryotoxicity assay showed a decreased overall toxicity, particularly cardiotoxicity and increased safety of the nanocomposite in comparison to doxorubicin alone, as manifested by a higher survival of embryos and less pericardial edema.
T2  - RSC Advances
T1  - Fullerenol nanoparticles as a new delivery system for doxorubicin
VL  - 6
IS  - 45
SP  - 38563
EP  - 38578
DO  - 10.1039/c6ra03879d
ER  - 

@article{
author = "Jović, Danica S. and Seke, Mariana and Đorđević, Aleksandar N. and Mrđanović, Jasminka Ž. and Aleksić, Lidija D. and Bogdanović, Gordana M. and Pavić, Aleksandar B. and Plavec, Janez",
year = "2016",
abstract = "Doxorubicin is a very potent chemotherapeutic drug, however its side effects limit its clinical use. The aim of this research was to investigate the properties of a fullerenol/doxorubicin nanocomposite, its potentially cytotoxic and genotoxic effects on malignant cell lines, as well as its toxicity towards zebra fish embryos. Chromatographic, NMR and mass spectral analysis of the nanocomposite imply that interactions between doxorubicin and fullerenol are non-covalent bonds. The stability of the nanocomposite was confirmed by the use of atomic force microscopy, dynamic light scattering and transmission electron microscopy. The nanocomposite, compared to the free doxorubicin at equivalent concentrations, significantly decreased the viability of MCF-7 and MDA-MB-231 cells. The flow cytometry results indicated that doxorubicin-loaded fullerenol could remarkably increase the uptake of doxorubicin suggesting that fullerenol might be a promising intracellular targeting carrier for the efficient delivery of antitumor drugs into tumor cells. The nanocomposite also affected cell cycle distribution. A genotoxicity test showed that the nanocomposite at all examined concentrations on MCF-7 and at lower concentrations on MDA-MB-231 cells caused DNA damage. Consequently, cell proliferation was notably reduced when compared with controls. Results of the zebrafish embryotoxicity assay showed a decreased overall toxicity, particularly cardiotoxicity and increased safety of the nanocomposite in comparison to doxorubicin alone, as manifested by a higher survival of embryos and less pericardial edema.",
journal = "RSC Advances",
title = "Fullerenol nanoparticles as a new delivery system for doxorubicin",
volume = "6",
number = "45",
pages = "38563-38578",
doi = "10.1039/c6ra03879d"
}

Jović, D. S., Seke, M., Đorđević, A. N., Mrđanović, J. Ž., Aleksić, L. D., Bogdanović, G. M., Pavić, A. B.,& Plavec, J.. (2016). Fullerenol nanoparticles as a new delivery system for doxorubicin. in RSC Advances, 6(45), 38563-38578.
https://doi.org/10.1039/c6ra03879d

Jović DS, Seke M, Đorđević AN, Mrđanović JŽ, Aleksić LD, Bogdanović GM, Pavić AB, Plavec J. Fullerenol nanoparticles as a new delivery system for doxorubicin. in RSC Advances. 2016;6(45):38563-38578.
doi:10.1039/c6ra03879d .

Jović, Danica S., Seke, Mariana, Đorđević, Aleksandar N., Mrđanović, Jasminka Ž., Aleksić, Lidija D., Bogdanović, Gordana M., Pavić, Aleksandar B., Plavec, Janez, "Fullerenol nanoparticles as a new delivery system for doxorubicin" in RSC Advances, 6, no. 45 (2016):38563-38578,
https://doi.org/10.1039/c6ra03879d . .