TY  - JOUR
AU  - Arsić, Biljana
AU  - Petrović, Stefan
AU  - Mrmošanin, Jelena
AU  - Dimitrijević, Ivana
AU  - Tošić, Snežana
AU  - Stojanović, Gordana
AU  - Glišić, Sanja
AU  - Milićević, Jelena
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12852
AB  - Pesticides commonly used in the Republic of Serbia (tebuconazole, pendimethalin, pyraclostrobin, propiconazole, and famoxadone) have high stability, so their potential toxicity to humans needs to be investigated. These pesticides are in use in the Republic of Serbia in various formulations. Their toxicity and interactions with acetylcholinesterase were thoroughly investigated in this study using computational tools. The ADMET (Adsorption, Distribution, Metabolism, Excretion, Toxicity) study showed that all of them are good oral compounds, and that pendimethalin was a mutagenic compound. Glide scores ranged from -18.41 kJ mol-1 (pendimethalin) to -27.61 kJ mol-1 (famoxadone) in Mus musculus, and from -19.58 kJ mol-1 (pendimethalin) to -24.31 kJ mol-1 (propiconazole) in Homo sapiens. In addition, the experimental stability of the pesticides solutions in methanol was studied using the fast GC-MS (retention times of the studied pesticides ranged from 14.47 min (pendimethalin) to 22 min (famoxadone)). They showed good stability over time, apart from pyraclostrobin which decomposed mainly into its des-methoxy derivative after 20 months. Based on the promising modeling results, pyraclostrobin and famoxadone emerge as potential candidates for further investigation in the treatment of Alzheimer's disease, taking care to increase their stability.
AB  - Пестициди који се често користе у Републици Србији (тебуконазол, пендиметалин, пираклостробин, пропиконазол, и фамоксадон) имају високу стабилност, тако да њихова потенцијална токсичност се мора проучити. Ови пестициди се користе у Републици Србији у различитим формулацијама. Њихова токсичност и интеракције са ацетихолинестеразом су детаљно изучене у овом истраживању коришћењем компјутерских алата. АДМЕТ (Адсорпција, Дистрибуција, Метаболизам, Екскреција, Токсичност) изучавање је показало да су сви они добра орална једињења, и да је пендиметалин мутагено једињење. Глајд скорови су били у опсегу од -18,41 kJ mol -1 (пендиметалин) до -27,61 kJ mol-1 (фамоксадон) код миша, и од -19,58 kJ mol-1 (пендиметалин) до -24,31 kJ mol-1 (пропиконазол) код човека. Осим тога, експериментална стабилност раствора пестицида у метанолу је проучавана коришћењем ГХ-МС (ретенциона времена проучаваних пестицида се кретала од 14.47 мин (пендиметалин) до 22 мин (фамоксадон)). Они показују добру стабилност током времена, осим пираклостробина који се углавном распада на свој дез-метокси дериват после 20 месеци. На основу обећавајућих резултата моделовања, пираклостробин и фамоксадон се појављују као потенцијални кандидати који се могу даље изучавати као третман за Алцхајмерову болест, водећи рачина да се повећа њихова стабилност.
T2  - Journal of the Serbian Chemical Society
T1  - Stability and computational analyses of selected pesticides in use in the Republic of Serbia
VL  - 89
IS  - 2
SP  - 259
EP  - 274
DO  - 10.2298/JSC230714102A
ER  - 

@article{
author = "Arsić, Biljana and Petrović, Stefan and Mrmošanin, Jelena and Dimitrijević, Ivana and Tošić, Snežana and Stojanović, Gordana and Glišić, Sanja and Milićević, Jelena",
year = "2024",
abstract = "Pesticides commonly used in the Republic of Serbia (tebuconazole, pendimethalin, pyraclostrobin, propiconazole, and famoxadone) have high stability, so their potential toxicity to humans needs to be investigated. These pesticides are in use in the Republic of Serbia in various formulations. Their toxicity and interactions with acetylcholinesterase were thoroughly investigated in this study using computational tools. The ADMET (Adsorption, Distribution, Metabolism, Excretion, Toxicity) study showed that all of them are good oral compounds, and that pendimethalin was a mutagenic compound. Glide scores ranged from -18.41 kJ mol-1 (pendimethalin) to -27.61 kJ mol-1 (famoxadone) in Mus musculus, and from -19.58 kJ mol-1 (pendimethalin) to -24.31 kJ mol-1 (propiconazole) in Homo sapiens. In addition, the experimental stability of the pesticides solutions in methanol was studied using the fast GC-MS (retention times of the studied pesticides ranged from 14.47 min (pendimethalin) to 22 min (famoxadone)). They showed good stability over time, apart from pyraclostrobin which decomposed mainly into its des-methoxy derivative after 20 months. Based on the promising modeling results, pyraclostrobin and famoxadone emerge as potential candidates for further investigation in the treatment of Alzheimer's disease, taking care to increase their stability., Пестициди који се често користе у Републици Србији (тебуконазол, пендиметалин, пираклостробин, пропиконазол, и фамоксадон) имају високу стабилност, тако да њихова потенцијална токсичност се мора проучити. Ови пестициди се користе у Републици Србији у различитим формулацијама. Њихова токсичност и интеракције са ацетихолинестеразом су детаљно изучене у овом истраживању коришћењем компјутерских алата. АДМЕТ (Адсорпција, Дистрибуција, Метаболизам, Екскреција, Токсичност) изучавање је показало да су сви они добра орална једињења, и да је пендиметалин мутагено једињење. Глајд скорови су били у опсегу од -18,41 kJ mol -1 (пендиметалин) до -27,61 kJ mol-1 (фамоксадон) код миша, и од -19,58 kJ mol-1 (пендиметалин) до -24,31 kJ mol-1 (пропиконазол) код човека. Осим тога, експериментална стабилност раствора пестицида у метанолу је проучавана коришћењем ГХ-МС (ретенциона времена проучаваних пестицида се кретала од 14.47 мин (пендиметалин) до 22 мин (фамоксадон)). Они показују добру стабилност током времена, осим пираклостробина који се углавном распада на свој дез-метокси дериват после 20 месеци. На основу обећавајућих резултата моделовања, пираклостробин и фамоксадон се појављују као потенцијални кандидати који се могу даље изучавати као третман за Алцхајмерову болест, водећи рачина да се повећа њихова стабилност.",
journal = "Journal of the Serbian Chemical Society",
title = "Stability and computational analyses of selected pesticides in use in the Republic of Serbia",
volume = "89",
number = "2",
pages = "259-274",
doi = "10.2298/JSC230714102A"
}

Arsić, B., Petrović, S., Mrmošanin, J., Dimitrijević, I., Tošić, S., Stojanović, G., Glišić, S.,& Milićević, J.. (2024). Stability and computational analyses of selected pesticides in use in the Republic of Serbia. in Journal of the Serbian Chemical Society, 89(2), 259-274.
https://doi.org/10.2298/JSC230714102A

Arsić B, Petrović S, Mrmošanin J, Dimitrijević I, Tošić S, Stojanović G, Glišić S, Milićević J. Stability and computational analyses of selected pesticides in use in the Republic of Serbia. in Journal of the Serbian Chemical Society. 2024;89(2):259-274.
doi:10.2298/JSC230714102A .

Arsić, Biljana, Petrović, Stefan, Mrmošanin, Jelena, Dimitrijević, Ivana, Tošić, Snežana, Stojanović, Gordana, Glišić, Sanja, Milićević, Jelena, "Stability and computational analyses of selected pesticides in use in the Republic of Serbia" in Journal of the Serbian Chemical Society, 89, no. 2 (2024):259-274,
https://doi.org/10.2298/JSC230714102A . .

TY  - CONF
AU  - Milićević, Jelena
AU  - Arsić, Biljana
AU  - Petrović, Stefan
AU  - Tošić, Snežana
AU  - Dimitrijević, Ivana
AU  - Stojanović, Gordana
AU  - Glišić, Sanja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11585
PB  - Slovensko kemijsko društvo : Ljubljana, Slovenia
C3  - Slovenski kemijski dnevi
T1  - In silico and stability analyses of selected pesticides in use in Serbia
SP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11585
ER  - 

@conference{
author = "Milićević, Jelena and Arsić, Biljana and Petrović, Stefan and Tošić, Snežana and Dimitrijević, Ivana and Stojanović, Gordana and Glišić, Sanja",
year = "2023",
publisher = "Slovensko kemijsko društvo : Ljubljana, Slovenia",
journal = "Slovenski kemijski dnevi",
title = "In silico and stability analyses of selected pesticides in use in Serbia",
pages = "133",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11585"
}

Milićević, J., Arsić, B., Petrović, S., Tošić, S., Dimitrijević, I., Stojanović, G.,& Glišić, S.. (2023). In silico and stability analyses of selected pesticides in use in Serbia. in Slovenski kemijski dnevi
Slovensko kemijsko društvo : Ljubljana, Slovenia., 133.
https://hdl.handle.net/21.15107/rcub_vinar_11585

Milićević J, Arsić B, Petrović S, Tošić S, Dimitrijević I, Stojanović G, Glišić S. In silico and stability analyses of selected pesticides in use in Serbia. in Slovenski kemijski dnevi. 2023;:133.
https://hdl.handle.net/21.15107/rcub_vinar_11585 .

Milićević, Jelena, Arsić, Biljana, Petrović, Stefan, Tošić, Snežana, Dimitrijević, Ivana, Stojanović, Gordana, Glišić, Sanja, "In silico and stability analyses of selected pesticides in use in Serbia" in Slovenski kemijski dnevi (2023):133,
https://hdl.handle.net/21.15107/rcub_vinar_11585 .

TY  - JOUR
AU  - Petrović, Stefan
AU  - Arsić, Biljana
AU  - Zlatanović, Ivana
AU  - Milićević, Jelena S.
AU  - Glišić, Sanja
AU  - Mitić, Milan
AU  - Đurović-Pejčev, Rada
AU  - Stojanović, Gordana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11025
AB  - In this study, we considered some pesticides as active substances within formulations for the protection of plant-based food in the Republic of Serbia in silico, because these pesticides have not often been investigated in this way previously, and in an analytical way, because there are not very many available fast, cheap, and easy methods for their determination in real agricultural samples. Seven pesticides were detected in selected agricultural products (tomatoes, cucumbers, peppers, and grapes) using the QuEChERS methodology and HPLC-DAD. Standard curves for the investigated pesticides (chlorantraniliprole, methomyl, metalaxyl, thiacloprid, acetamiprid, emamectin benzoate, and cymoxanil) show good linearity, with R2 values from 0.9785 to 0.9996. The HPLC-DAD method is fast, and these pesticides can be determined in real spiked samples in less than 15 min. We further characterized the pesticides we found in food based on physicochemical properties and molecular descriptors to predict the absorption, distribution, metabolism, elimination, and toxicity (ADMET) of the compounds. We summarized the data supporting their effects on humans using various computational tools to determine their potential adverse effects. The results of our prediction study show that all of the selected pesticides considered in this study have good oral bioavailability, and those with high toxicity, therefore, could be harmful to human health. Chlorantraniliprole was shown in a molecular docking study as a good starting point for a new Alzheimer’s disease drug candidate.
T2  - International Journal of Molecular Sciences
T1  - In Silico Investigation of Selected Pesticides and Their Determination in Agricultural Products Using QuEChERS Methodology and HPLC-DAD
VL  - 24
IS  - 9
SP  - 8003
DO  - 10.3390/ijms24098003
ER  - 

@article{
author = "Petrović, Stefan and Arsić, Biljana and Zlatanović, Ivana and Milićević, Jelena S. and Glišić, Sanja and Mitić, Milan and Đurović-Pejčev, Rada and Stojanović, Gordana",
year = "2023",
abstract = "In this study, we considered some pesticides as active substances within formulations for the protection of plant-based food in the Republic of Serbia in silico, because these pesticides have not often been investigated in this way previously, and in an analytical way, because there are not very many available fast, cheap, and easy methods for their determination in real agricultural samples. Seven pesticides were detected in selected agricultural products (tomatoes, cucumbers, peppers, and grapes) using the QuEChERS methodology and HPLC-DAD. Standard curves for the investigated pesticides (chlorantraniliprole, methomyl, metalaxyl, thiacloprid, acetamiprid, emamectin benzoate, and cymoxanil) show good linearity, with R2 values from 0.9785 to 0.9996. The HPLC-DAD method is fast, and these pesticides can be determined in real spiked samples in less than 15 min. We further characterized the pesticides we found in food based on physicochemical properties and molecular descriptors to predict the absorption, distribution, metabolism, elimination, and toxicity (ADMET) of the compounds. We summarized the data supporting their effects on humans using various computational tools to determine their potential adverse effects. The results of our prediction study show that all of the selected pesticides considered in this study have good oral bioavailability, and those with high toxicity, therefore, could be harmful to human health. Chlorantraniliprole was shown in a molecular docking study as a good starting point for a new Alzheimer’s disease drug candidate.",
journal = "International Journal of Molecular Sciences",
title = "In Silico Investigation of Selected Pesticides and Their Determination in Agricultural Products Using QuEChERS Methodology and HPLC-DAD",
volume = "24",
number = "9",
pages = "8003",
doi = "10.3390/ijms24098003"
}

Petrović, S., Arsić, B., Zlatanović, I., Milićević, J. S., Glišić, S., Mitić, M., Đurović-Pejčev, R.,& Stojanović, G.. (2023). In Silico Investigation of Selected Pesticides and Their Determination in Agricultural Products Using QuEChERS Methodology and HPLC-DAD. in International Journal of Molecular Sciences, 24(9), 8003.
https://doi.org/10.3390/ijms24098003

Petrović S, Arsić B, Zlatanović I, Milićević JS, Glišić S, Mitić M, Đurović-Pejčev R, Stojanović G. In Silico Investigation of Selected Pesticides and Their Determination in Agricultural Products Using QuEChERS Methodology and HPLC-DAD. in International Journal of Molecular Sciences. 2023;24(9):8003.
doi:10.3390/ijms24098003 .

Petrović, Stefan, Arsić, Biljana, Zlatanović, Ivana, Milićević, Jelena S., Glišić, Sanja, Mitić, Milan, Đurović-Pejčev, Rada, Stojanović, Gordana, "In Silico Investigation of Selected Pesticides and Their Determination in Agricultural Products Using QuEChERS Methodology and HPLC-DAD" in International Journal of Molecular Sciences, 24, no. 9 (2023):8003,
https://doi.org/10.3390/ijms24098003 . .

TY  - CONF
AU  - Stanković, Miroslav
AU  - Stojanović, Igor
AU  - Zlatanović, Ivana
AU  - Milovanović, Vesna
AU  - Stojanović, Gordana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11147
PB  - RAD Centre, Niš, Serbia
C3  - RAD 2019 : 7th International Conference on Radiation in Various Fields of Research : book of abstracts; June 10-14, 2019; Herceg Novi, Montenegro
T1  - An overview of the effect of Hypogimnia physodes, Hypogimnia tubulosa, Umbilicaria crustulosa and Umbilicaria cylindrica acetone extracts on frequencies and distribution of micronucleus in human lymphocytes
SP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11147
ER  - 

@conference{
author = "Stanković, Miroslav and Stojanović, Igor and Zlatanović, Ivana and Milovanović, Vesna and Stojanović, Gordana",
year = "2019",
publisher = "RAD Centre, Niš, Serbia",
journal = "RAD 2019 : 7th International Conference on Radiation in Various Fields of Research : book of abstracts; June 10-14, 2019; Herceg Novi, Montenegro",
title = "An overview of the effect of Hypogimnia physodes, Hypogimnia tubulosa, Umbilicaria crustulosa and Umbilicaria cylindrica acetone extracts on frequencies and distribution of micronucleus in human lymphocytes",
pages = "140",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11147"
}

Stanković, M., Stojanović, I., Zlatanović, I., Milovanović, V.,& Stojanović, G.. (2019). An overview of the effect of Hypogimnia physodes, Hypogimnia tubulosa, Umbilicaria crustulosa and Umbilicaria cylindrica acetone extracts on frequencies and distribution of micronucleus in human lymphocytes. in RAD 2019 : 7th International Conference on Radiation in Various Fields of Research : book of abstracts; June 10-14, 2019; Herceg Novi, Montenegro
RAD Centre, Niš, Serbia., 140.
https://hdl.handle.net/21.15107/rcub_vinar_11147

Stanković M, Stojanović I, Zlatanović I, Milovanović V, Stojanović G. An overview of the effect of Hypogimnia physodes, Hypogimnia tubulosa, Umbilicaria crustulosa and Umbilicaria cylindrica acetone extracts on frequencies and distribution of micronucleus in human lymphocytes. in RAD 2019 : 7th International Conference on Radiation in Various Fields of Research : book of abstracts; June 10-14, 2019; Herceg Novi, Montenegro. 2019;:140.
https://hdl.handle.net/21.15107/rcub_vinar_11147 .

Stanković, Miroslav, Stojanović, Igor, Zlatanović, Ivana, Milovanović, Vesna, Stojanović, Gordana, "An overview of the effect of Hypogimnia physodes, Hypogimnia tubulosa, Umbilicaria crustulosa and Umbilicaria cylindrica acetone extracts on frequencies and distribution of micronucleus in human lymphocytes" in RAD 2019 : 7th International Conference on Radiation in Various Fields of Research : book of abstracts; June 10-14, 2019; Herceg Novi, Montenegro (2019):140,
https://hdl.handle.net/21.15107/rcub_vinar_11147 .

TY  - JOUR
AU  - Zlatanović, Ivana
AU  - Stanković, Miroslava
AU  - Stankov Jovanović, Vesna
AU  - Mitić, Violeta
AU  - Zrnzevic, Ivana
AU  - Đorđević, Aleksandra
AU  - Stojanović, Gordana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1466
AB  - This paper reports for the first time the effect of an acetone extract of Umbilicaria crustulosa on the micronucleus distribution of human lymphocytes, and on the cholinesterase activity and antioxidant activity by the cupric ion reducing antioxidant capacity (CUPRAC) method. Additionally, the total phenolic compounds (TPC) and the antioxidant properties were estimated via DPPH, ABTS and TRP assays. Moreover, the antibacterial activity against two Gram-positive and three Gram-negative bacteria were determined. Acetone extract of U. crustulosa at concentration of 1 and 2 mu g mL(-1) decreased a frequency of micronuclei (MN) by 10.8 and 16.8 %, respectively, acting more or slightly less than the synthetic protector amifostine (AMF, WR-2721, 11.4 %, at concentration of 1 mu g mL(-1)). The tested extract did not inhibit cholinesterase activity nor did it exhibit activity toward the examined bacteria. The extract reduced the concentration of DPPH and ABTS radicals by 88.7 and 96.2 %, respectively. Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.6197+/-0.0166 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 19.7641+/-1.6546 mu g trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 350.4188 +/-14.587 mu g gallic acid equivalents (GAE) per mg of dry extract. The results of the present study showed that U. crustulosa acetone extract is a promising candidate for in vivo experiments considering its antioxidant activity and protective effect on human lymphocytes.
T2  - Journal of the Serbian Chemical Society
T1  - Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract
VL  - 82
IS  - 2
SP  - 141
EP  - 150
DO  - 10.2298/JSC161019014Z
ER  - 

@article{
author = "Zlatanović, Ivana and Stanković, Miroslava and Stankov Jovanović, Vesna and Mitić, Violeta and Zrnzevic, Ivana and Đorđević, Aleksandra and Stojanović, Gordana",
year = "2017",
abstract = "This paper reports for the first time the effect of an acetone extract of Umbilicaria crustulosa on the micronucleus distribution of human lymphocytes, and on the cholinesterase activity and antioxidant activity by the cupric ion reducing antioxidant capacity (CUPRAC) method. Additionally, the total phenolic compounds (TPC) and the antioxidant properties were estimated via DPPH, ABTS and TRP assays. Moreover, the antibacterial activity against two Gram-positive and three Gram-negative bacteria were determined. Acetone extract of U. crustulosa at concentration of 1 and 2 mu g mL(-1) decreased a frequency of micronuclei (MN) by 10.8 and 16.8 %, respectively, acting more or slightly less than the synthetic protector amifostine (AMF, WR-2721, 11.4 %, at concentration of 1 mu g mL(-1)). The tested extract did not inhibit cholinesterase activity nor did it exhibit activity toward the examined bacteria. The extract reduced the concentration of DPPH and ABTS radicals by 88.7 and 96.2 %, respectively. Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.6197+/-0.0166 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 19.7641+/-1.6546 mu g trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 350.4188 +/-14.587 mu g gallic acid equivalents (GAE) per mg of dry extract. The results of the present study showed that U. crustulosa acetone extract is a promising candidate for in vivo experiments considering its antioxidant activity and protective effect on human lymphocytes.",
journal = "Journal of the Serbian Chemical Society",
title = "Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract",
volume = "82",
number = "2",
pages = "141-150",
doi = "10.2298/JSC161019014Z"
}

Zlatanović, I., Stanković, M., Stankov Jovanović, V., Mitić, V., Zrnzevic, I., Đorđević, A.,& Stojanović, G.. (2017). Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract. in Journal of the Serbian Chemical Society, 82(2), 141-150.
https://doi.org/10.2298/JSC161019014Z

Zlatanović I, Stanković M, Stankov Jovanović V, Mitić V, Zrnzevic I, Đorđević A, Stojanović G. Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract. in Journal of the Serbian Chemical Society. 2017;82(2):141-150.
doi:10.2298/JSC161019014Z .

Zlatanović, Ivana, Stanković, Miroslava, Stankov Jovanović, Vesna, Mitić, Violeta, Zrnzevic, Ivana, Đorđević, Aleksandra, Stojanović, Gordana, "Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract" in Journal of the Serbian Chemical Society, 82, no. 2 (2017):141-150,
https://doi.org/10.2298/JSC161019014Z . .

TY  - JOUR
AU  - Zrnzevic, Ivana
AU  - Stanković, Miroslava
AU  - Stankov Jovanović, Vesna
AU  - Mitić, Violeta
AU  - Đorđević, Aleksandra
AU  - Zlatanović, Ivana
AU  - Stojanović, Gordana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1656
AB  - In the present investigation, effects of Ramalina capitata acetone extract on micronucleus distribution on human lymphocytes, on cholinesterase activity and antioxidant activity (by the CUPRAC method) were examined, for the first time as well as its HPLC profile. Additionally, total phenolic compounds (TPC), antioxidant properties (estimated via DPPH, ABTS and TRP assays) and antibacterial activity were determined. The predominant phenolic compounds in this extract were evernic, everninic and obtusatic acids. Acetone extract of R. capitata at concentration of 2 mu g mL(-1) decreased a frequency of micronuclei (MN) for 14.8 %. The extract reduces the concentration of DPPH and ABTS radicals for 21.2 and 36.1 % (respectively). Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.4624 +/- 0.1064 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 6.1176 +/- 0.2964 mu g Trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 670.6376 +/- 66.554 mu g galic acid equivalents (GAE) per mg of dry extract. Tested extract at concentration of 2 mg mL(-1) exhibited inhibition effect (5.2 %) on pooled human serum cholinesterase. The antimicrobial assay showed that acetone extract had inhibition effect towards Gram-positive strains. The results of manifested antioxidant activity, reducing the number of micronuclei in human lymphocytes, and antibacterial activity recommends R. capitata extract for further in vivo studies.
T2  - EXCLI Journal
T1  - Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity
VL  - 16
SP  - 679
EP  - 687
DO  - 10.17179/excli2017-301
ER  - 

@article{
author = "Zrnzevic, Ivana and Stanković, Miroslava and Stankov Jovanović, Vesna and Mitić, Violeta and Đorđević, Aleksandra and Zlatanović, Ivana and Stojanović, Gordana",
year = "2017",
abstract = "In the present investigation, effects of Ramalina capitata acetone extract on micronucleus distribution on human lymphocytes, on cholinesterase activity and antioxidant activity (by the CUPRAC method) were examined, for the first time as well as its HPLC profile. Additionally, total phenolic compounds (TPC), antioxidant properties (estimated via DPPH, ABTS and TRP assays) and antibacterial activity were determined. The predominant phenolic compounds in this extract were evernic, everninic and obtusatic acids. Acetone extract of R. capitata at concentration of 2 mu g mL(-1) decreased a frequency of micronuclei (MN) for 14.8 %. The extract reduces the concentration of DPPH and ABTS radicals for 21.2 and 36.1 % (respectively). Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.4624 +/- 0.1064 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 6.1176 +/- 0.2964 mu g Trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 670.6376 +/- 66.554 mu g galic acid equivalents (GAE) per mg of dry extract. Tested extract at concentration of 2 mg mL(-1) exhibited inhibition effect (5.2 %) on pooled human serum cholinesterase. The antimicrobial assay showed that acetone extract had inhibition effect towards Gram-positive strains. The results of manifested antioxidant activity, reducing the number of micronuclei in human lymphocytes, and antibacterial activity recommends R. capitata extract for further in vivo studies.",
journal = "EXCLI Journal",
title = "Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity",
volume = "16",
pages = "679-687",
doi = "10.17179/excli2017-301"
}

Zrnzevic, I., Stanković, M., Stankov Jovanović, V., Mitić, V., Đorđević, A., Zlatanović, I.,& Stojanović, G.. (2017). Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity. in EXCLI Journal, 16, 679-687.
https://doi.org/10.17179/excli2017-301

Zrnzevic I, Stanković M, Stankov Jovanović V, Mitić V, Đorđević A, Zlatanović I, Stojanović G. Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity. in EXCLI Journal. 2017;16:679-687.
doi:10.17179/excli2017-301 .

Zrnzevic, Ivana, Stanković, Miroslava, Stankov Jovanović, Vesna, Mitić, Violeta, Đorđević, Aleksandra, Zlatanović, Ivana, Stojanović, Gordana, "Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity" in EXCLI Journal, 16 (2017):679-687,
https://doi.org/10.17179/excli2017-301 . .

TY  - JOUR
AU  - Stojanović, Gordana
AU  - Stanković, Miroslava
AU  - Stojanovic, Igor Z.
AU  - Palic, Ivan
AU  - Milovanović, Vesna
AU  - Rancic, Sofija
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5969
AB  - Three lichen secondary metabolites atranorin (1), evernic acid (2), and usnic acid (3), were evaluated for their in vitro clastogenic and antiproliferative effects on human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay at concentrations of 2 mu g/mL, 4 mu g/mL and 6 mu g/mL of final culture solution. The frequency of micronucleus (MN) was scored in binucleated cells, and cytokinesis-block proliferation index (CBPI) was calculated. Among the tested compounds, 3 exhibited the most prominent effect decreasing the frequency of MN in the range of 42.5% - 48.9%, that is about double of the positive control amifostin WR-2721 that reduces MN frequency for 22.0%. The effect of evernic acid was approximately equal to action of amifostin (23.2% -32.9%). Atranorin at concentrations of 2 mu g/mL and 4 mu g/mL decreasing the frequency of MN only for 11.1% and 1.8%, while in concentration of 6 mu g/mL increases the frequency of MN for 9.6 %. The comparable CBPI values of the investigated compounds and control suggested that they did not show a statistically significant inhibitory effect on lymphocyte cell proliferation at applied concentrations.
T2  - Natural Product Communications
T1  - Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes
VL  - 9
IS  - 4
SP  - 503
EP  - 504
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5969
ER  - 

@article{
author = "Stojanović, Gordana and Stanković, Miroslava and Stojanovic, Igor Z. and Palic, Ivan and Milovanović, Vesna and Rancic, Sofija",
year = "2014",
abstract = "Three lichen secondary metabolites atranorin (1), evernic acid (2), and usnic acid (3), were evaluated for their in vitro clastogenic and antiproliferative effects on human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay at concentrations of 2 mu g/mL, 4 mu g/mL and 6 mu g/mL of final culture solution. The frequency of micronucleus (MN) was scored in binucleated cells, and cytokinesis-block proliferation index (CBPI) was calculated. Among the tested compounds, 3 exhibited the most prominent effect decreasing the frequency of MN in the range of 42.5% - 48.9%, that is about double of the positive control amifostin WR-2721 that reduces MN frequency for 22.0%. The effect of evernic acid was approximately equal to action of amifostin (23.2% -32.9%). Atranorin at concentrations of 2 mu g/mL and 4 mu g/mL decreasing the frequency of MN only for 11.1% and 1.8%, while in concentration of 6 mu g/mL increases the frequency of MN for 9.6 %. The comparable CBPI values of the investigated compounds and control suggested that they did not show a statistically significant inhibitory effect on lymphocyte cell proliferation at applied concentrations.",
journal = "Natural Product Communications",
title = "Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes",
volume = "9",
number = "4",
pages = "503-504",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5969"
}

Stojanović, G., Stanković, M., Stojanovic, I. Z., Palic, I., Milovanović, V.,& Rancic, S.. (2014). Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes. in Natural Product Communications, 9(4), 503-504.
https://hdl.handle.net/21.15107/rcub_vinar_5969

Stojanović G, Stanković M, Stojanovic IZ, Palic I, Milovanović V, Rancic S. Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes. in Natural Product Communications. 2014;9(4):503-504.
https://hdl.handle.net/21.15107/rcub_vinar_5969 .

Stojanović, Gordana, Stanković, Miroslava, Stojanovic, Igor Z., Palic, Ivan, Milovanović, Vesna, Rancic, Sofija, "Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes" in Natural Product Communications, 9, no. 4 (2014):503-504,
https://hdl.handle.net/21.15107/rcub_vinar_5969 .

TY  - JOUR
AU  - Stojanovic, Igor Z.
AU  - Stanković, Miroslava
AU  - Jovanovic, Olga
AU  - Petrovic, Goran
AU  - Smelcerovic, Andrija
AU  - Stojanović, Gordana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5302
AB  - Three lichen depsidones, physodalic acid (1), physodic acid (2), and 3-hydroxy physodic acid (3), were isolated from Hypogymnia physodes diethyl ether extract using column chromatography, and their structures determined by comparing their UV, H-1 and C-13 NMR spectroscopic and MS data with those given in the literature, as well as with data computed by CHEM draw ultra 11 software. The contents of 1, 2 and 3 were determined in the methanol (ME), acetone (AE), and diethyl ether (EE) extracts using reversed-phase high performance liquid chromatography with photodiode array detection. The extracts, isolated depsidones 1-3 and EE fraction F23 (consisting of 90% 2 and 3, in the ratio 5.5: 1) were evaluated for their in vitro effects on chromosome aberrations in peripheral human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay in doses of 1 mu g/mL and 2 mu g/mL of final culture solution. The frequency of MN was scored in binucleated cells, and nuclear proliferation index was calculated. It was found that 1, 2, 3, F23, and EE at 1.0 mu g/mL exerted a beneficial effect on lymphocyte cells giving a significant decrease of the frequency of MN in comparison with the positive control Amifostin WR-2721. Among the tested depsidones at a concentration of 1 mu g/mL, 3 exhibited the most prominent effect decreasing the frequency of MN by 30.3%, followed by 2 (28.2%) and 1 (22.0%). The extracts were less effective than the isolated depsidones.
T2  - Natural Product Communications
T1  - Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes
VL  - 8
IS  - 1
SP  - 109
EP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5302
ER  - 

@article{
author = "Stojanovic, Igor Z. and Stanković, Miroslava and Jovanovic, Olga and Petrovic, Goran and Smelcerovic, Andrija and Stojanović, Gordana",
year = "2013",
abstract = "Three lichen depsidones, physodalic acid (1), physodic acid (2), and 3-hydroxy physodic acid (3), were isolated from Hypogymnia physodes diethyl ether extract using column chromatography, and their structures determined by comparing their UV, H-1 and C-13 NMR spectroscopic and MS data with those given in the literature, as well as with data computed by CHEM draw ultra 11 software. The contents of 1, 2 and 3 were determined in the methanol (ME), acetone (AE), and diethyl ether (EE) extracts using reversed-phase high performance liquid chromatography with photodiode array detection. The extracts, isolated depsidones 1-3 and EE fraction F23 (consisting of 90% 2 and 3, in the ratio 5.5: 1) were evaluated for their in vitro effects on chromosome aberrations in peripheral human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay in doses of 1 mu g/mL and 2 mu g/mL of final culture solution. The frequency of MN was scored in binucleated cells, and nuclear proliferation index was calculated. It was found that 1, 2, 3, F23, and EE at 1.0 mu g/mL exerted a beneficial effect on lymphocyte cells giving a significant decrease of the frequency of MN in comparison with the positive control Amifostin WR-2721. Among the tested depsidones at a concentration of 1 mu g/mL, 3 exhibited the most prominent effect decreasing the frequency of MN by 30.3%, followed by 2 (28.2%) and 1 (22.0%). The extracts were less effective than the isolated depsidones.",
journal = "Natural Product Communications",
title = "Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes",
volume = "8",
number = "1",
pages = "109-112",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5302"
}

Stojanovic, I. Z., Stanković, M., Jovanovic, O., Petrovic, G., Smelcerovic, A.,& Stojanović, G.. (2013). Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes. in Natural Product Communications, 8(1), 109-112.
https://hdl.handle.net/21.15107/rcub_vinar_5302

Stojanovic IZ, Stanković M, Jovanovic O, Petrovic G, Smelcerovic A, Stojanović G. Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes. in Natural Product Communications. 2013;8(1):109-112.
https://hdl.handle.net/21.15107/rcub_vinar_5302 .

Stojanovic, Igor Z., Stanković, Miroslava, Jovanovic, Olga, Petrovic, Goran, Smelcerovic, Andrija, Stojanović, Gordana, "Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes" in Natural Product Communications, 8, no. 1 (2013):109-112,
https://hdl.handle.net/21.15107/rcub_vinar_5302 .

TY  - JOUR
AU  - Milovanović, Vesna
AU  - Radulovic, Niko
AU  - Todorović, Zoran
AU  - Stanković, Miroslava
AU  - Stojanović, Gordana
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3279
AB  - The hydro-alcoholic extracts of five Equisetum species, E. arvense L., E. sylvaticum L., E. fluviatile L., E. palustre L. and E. telmateia Ehrh., growing-wild in Serbia were evaluated for their genotoxicity, antimicrobial activity, antioxidant capacity and the results related to the total phenol content and HPLC flavonoid profiles. The total phenol content was 92-349 mu mol expressed as equivalents of chlorogenic acid per g of dried plant material. Main identified compounds were kaempferol-, quercetin- glycosides and caffeic acid derivatives. E. telmateia extract showed the greatest antioxidant capacity. Almost all tested microorganisms demonstrated some degree of sensitivity to the examined extracts. All tested extracts at 62.5 mu g/ml showed higher incidence of micronucleus formation than in the control sample. The obtained data allowed mutual comparison of examined species and their assessment as possible sources of antioxidants, antimicrobials and/or genotoxic substances.
T2  - Plant Foods for Human Nutrition
T1  - Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extracts of five serbian Equisetum species
VL  - 62
IS  - 3
SP  - 113
EP  - 119
DO  - 10.1007/s11130-007-0050-z
ER  - 

@article{
author = "Milovanović, Vesna and Radulovic, Niko and Todorović, Zoran and Stanković, Miroslava and Stojanović, Gordana",
year = "2007",
abstract = "The hydro-alcoholic extracts of five Equisetum species, E. arvense L., E. sylvaticum L., E. fluviatile L., E. palustre L. and E. telmateia Ehrh., growing-wild in Serbia were evaluated for their genotoxicity, antimicrobial activity, antioxidant capacity and the results related to the total phenol content and HPLC flavonoid profiles. The total phenol content was 92-349 mu mol expressed as equivalents of chlorogenic acid per g of dried plant material. Main identified compounds were kaempferol-, quercetin- glycosides and caffeic acid derivatives. E. telmateia extract showed the greatest antioxidant capacity. Almost all tested microorganisms demonstrated some degree of sensitivity to the examined extracts. All tested extracts at 62.5 mu g/ml showed higher incidence of micronucleus formation than in the control sample. The obtained data allowed mutual comparison of examined species and their assessment as possible sources of antioxidants, antimicrobials and/or genotoxic substances.",
journal = "Plant Foods for Human Nutrition",
title = "Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extracts of five serbian Equisetum species",
volume = "62",
number = "3",
pages = "113-119",
doi = "10.1007/s11130-007-0050-z"
}

Milovanović, V., Radulovic, N., Todorović, Z., Stanković, M.,& Stojanović, G.. (2007). Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extracts of five serbian Equisetum species. in Plant Foods for Human Nutrition, 62(3), 113-119.
https://doi.org/10.1007/s11130-007-0050-z

Milovanović V, Radulovic N, Todorović Z, Stanković M, Stojanović G. Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extracts of five serbian Equisetum species. in Plant Foods for Human Nutrition. 2007;62(3):113-119.
doi:10.1007/s11130-007-0050-z .

Milovanović, Vesna, Radulovic, Niko, Todorović, Zoran, Stanković, Miroslava, Stojanović, Gordana, "Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extracts of five serbian Equisetum species" in Plant Foods for Human Nutrition, 62, no. 3 (2007):113-119,
https://doi.org/10.1007/s11130-007-0050-z . .