TY  - JOUR
AU  - Prljić, Jelena
AU  - Veljković, Nevena V.
AU  - Doliana, R
AU  - Colombatti, A
AU  - Johnson, Emmett
AU  - Metlaš, Radmila
AU  - Veljković, Veljko
PY  - 1999
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2230
AB  - Because of a sequence similarity between the HIV-1 envelope glycoprotein gp120 and the variable region of human immunoglobulins, we have suggested that the use of this protein as a vaccine component could strongly influence the host immune system, making it more vulnerable to HIV, and in the long term, accelerate disease progression in asymptomatic HIV patients. Using a chimeric primer consisting of the nucleotide sequence derived from the HIV-1 env gene coding for the second conserved region of gp120, and the highly conserved sequence derived from the human immunoglobulin gene coding for the VHIII domain, we have identified in sera of AIDS patients HIV-I field isolates carrying the complete and active Chi recombinational hot spot (GCTGGTGG). We have also demonstrated in vivo recombination between the HIV-I gene coding for the central portion of the gp120 involving the V3 loop and the bacterial gene coding for the clp protease. These results strongly support and reinforce the previous contention and the serious concern that AIDS vaccine candidates carrying the HIV-1 env gene on viral and bacterial vectors, could result in the generation of new pathogens with unpredictable effects on the immune system. (C) 1999 Elsevier Science Ltd. All rights reserved.
T2  - Vaccine
T1  - Identification of an active Chi recombinational hot spot within the HIV-1 envelope gene: consequences for development of AIDS vaccines
VL  - 17
IS  - 11-12
SP  - 1462
EP  - 1467
DO  - 10.1016/S0264-410X(98)00373-9
ER  - 

@article{
author = "Prljić, Jelena and Veljković, Nevena V. and Doliana, R and Colombatti, A and Johnson, Emmett and Metlaš, Radmila and Veljković, Veljko",
year = "1999",
abstract = "Because of a sequence similarity between the HIV-1 envelope glycoprotein gp120 and the variable region of human immunoglobulins, we have suggested that the use of this protein as a vaccine component could strongly influence the host immune system, making it more vulnerable to HIV, and in the long term, accelerate disease progression in asymptomatic HIV patients. Using a chimeric primer consisting of the nucleotide sequence derived from the HIV-1 env gene coding for the second conserved region of gp120, and the highly conserved sequence derived from the human immunoglobulin gene coding for the VHIII domain, we have identified in sera of AIDS patients HIV-I field isolates carrying the complete and active Chi recombinational hot spot (GCTGGTGG). We have also demonstrated in vivo recombination between the HIV-I gene coding for the central portion of the gp120 involving the V3 loop and the bacterial gene coding for the clp protease. These results strongly support and reinforce the previous contention and the serious concern that AIDS vaccine candidates carrying the HIV-1 env gene on viral and bacterial vectors, could result in the generation of new pathogens with unpredictable effects on the immune system. (C) 1999 Elsevier Science Ltd. All rights reserved.",
journal = "Vaccine",
title = "Identification of an active Chi recombinational hot spot within the HIV-1 envelope gene: consequences for development of AIDS vaccines",
volume = "17",
number = "11-12",
pages = "1462-1467",
doi = "10.1016/S0264-410X(98)00373-9"
}

Prljić, J., Veljković, N. V., Doliana, R., Colombatti, A., Johnson, E., Metlaš, R.,& Veljković, V.. (1999). Identification of an active Chi recombinational hot spot within the HIV-1 envelope gene: consequences for development of AIDS vaccines. in Vaccine, 17(11-12), 1462-1467.
https://doi.org/10.1016/S0264-410X(98)00373-9

Prljić J, Veljković NV, Doliana R, Colombatti A, Johnson E, Metlaš R, Veljković V. Identification of an active Chi recombinational hot spot within the HIV-1 envelope gene: consequences for development of AIDS vaccines. in Vaccine. 1999;17(11-12):1462-1467.
doi:10.1016/S0264-410X(98)00373-9 .

Prljić, Jelena, Veljković, Nevena V., Doliana, R, Colombatti, A, Johnson, Emmett, Metlaš, Radmila, Veljković, Veljko, "Identification of an active Chi recombinational hot spot within the HIV-1 envelope gene: consequences for development of AIDS vaccines" in Vaccine, 17, no. 11-12 (1999):1462-1467,
https://doi.org/10.1016/S0264-410X(98)00373-9 . .