TY  - JOUR
AU  - Kurtovic, Nada Kraguljac
AU  - Krajnović, Milena M.
AU  - Bogdanović, Andrija
AU  - Suvajdzic, Nada
AU  - Jovanović, Jelica
AU  - Dimitrijević, Bogomir B.
AU  - Čolović, Milica
AU  - Krtolica-Žikić, Koviljka
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5173
AB  - In this study, methylation-specific polymerase chain reaction (MS-PCR) was used to define the methylation status of the target promoter sequences of p15 and MGMT genes in the group of 21 adult patients with acute myeloid leukemia (AML). The incidence of aberrant hypermethylation of p15 gene (71 %) was higher comparing to MGMT gene (33 %), whereas concomitant methylation of both genes had 24 % of the patients. Although the incidence of cytogenetic abnormalities between the groups with a different methylation status of p15 and/or MGMT genes was not significantly different, we observed general trend of clustering of abnormalities with adverse prognosis into groups with concomitant hypermethylation of both genes and only p15 gene. Also, we showed that AML patients with concomitant methylation of p15/MGMT genes had a higher proportion of leukemic blast cells characterized with specific expression of individual leukocyte surface antigens (CD117(+)/CD7(+)/CD34(+)/CD15(-)), indicating leukemic cells as early myeloid progenitors. Although we could not prove that hypermethylation of p15 and/or MGMT genes is predictive parameter for response to therapy and overall survival, we noticed that AML patients with comethylated p15/MGMT genes or methylated p15 gene exhibited a higher frequency of early death, lower frequency of complete remissions as well as a trend for shorter overall survival. Assessing of the methylation status of p15 and MGMT genes may allow stratification of patients with AML into distinct groups with potentially different prognosis.
T2  - Medical Oncology
T1  - Concomitant aberrant methylation of p15 and MGMT genes in acute myeloid leukemia: association with a particular immunophenotype of blast cells
VL  - 29
IS  - 5
SP  - 3547
EP  - 3556
DO  - 10.1007/s12032-012-0289-6
ER  - 

@article{
author = "Kurtovic, Nada Kraguljac and Krajnović, Milena M. and Bogdanović, Andrija and Suvajdzic, Nada and Jovanović, Jelica and Dimitrijević, Bogomir B. and Čolović, Milica and Krtolica-Žikić, Koviljka",
year = "2012",
abstract = "In this study, methylation-specific polymerase chain reaction (MS-PCR) was used to define the methylation status of the target promoter sequences of p15 and MGMT genes in the group of 21 adult patients with acute myeloid leukemia (AML). The incidence of aberrant hypermethylation of p15 gene (71 %) was higher comparing to MGMT gene (33 %), whereas concomitant methylation of both genes had 24 % of the patients. Although the incidence of cytogenetic abnormalities between the groups with a different methylation status of p15 and/or MGMT genes was not significantly different, we observed general trend of clustering of abnormalities with adverse prognosis into groups with concomitant hypermethylation of both genes and only p15 gene. Also, we showed that AML patients with concomitant methylation of p15/MGMT genes had a higher proportion of leukemic blast cells characterized with specific expression of individual leukocyte surface antigens (CD117(+)/CD7(+)/CD34(+)/CD15(-)), indicating leukemic cells as early myeloid progenitors. Although we could not prove that hypermethylation of p15 and/or MGMT genes is predictive parameter for response to therapy and overall survival, we noticed that AML patients with comethylated p15/MGMT genes or methylated p15 gene exhibited a higher frequency of early death, lower frequency of complete remissions as well as a trend for shorter overall survival. Assessing of the methylation status of p15 and MGMT genes may allow stratification of patients with AML into distinct groups with potentially different prognosis.",
journal = "Medical Oncology",
title = "Concomitant aberrant methylation of p15 and MGMT genes in acute myeloid leukemia: association with a particular immunophenotype of blast cells",
volume = "29",
number = "5",
pages = "3547-3556",
doi = "10.1007/s12032-012-0289-6"
}

Kurtovic, N. K., Krajnović, M. M., Bogdanović, A., Suvajdzic, N., Jovanović, J., Dimitrijević, B. B., Čolović, M.,& Krtolica-Žikić, K.. (2012). Concomitant aberrant methylation of p15 and MGMT genes in acute myeloid leukemia: association with a particular immunophenotype of blast cells. in Medical Oncology, 29(5), 3547-3556.
https://doi.org/10.1007/s12032-012-0289-6

Kurtovic NK, Krajnović MM, Bogdanović A, Suvajdzic N, Jovanović J, Dimitrijević BB, Čolović M, Krtolica-Žikić K. Concomitant aberrant methylation of p15 and MGMT genes in acute myeloid leukemia: association with a particular immunophenotype of blast cells. in Medical Oncology. 2012;29(5):3547-3556.
doi:10.1007/s12032-012-0289-6 .

Kurtovic, Nada Kraguljac, Krajnović, Milena M., Bogdanović, Andrija, Suvajdzic, Nada, Jovanović, Jelica, Dimitrijević, Bogomir B., Čolović, Milica, Krtolica-Žikić, Koviljka, "Concomitant aberrant methylation of p15 and MGMT genes in acute myeloid leukemia: association with a particular immunophenotype of blast cells" in Medical Oncology, 29, no. 5 (2012):3547-3556,
https://doi.org/10.1007/s12032-012-0289-6 . .

TY  - JOUR
AU  - Todorić-Živanović, B.
AU  - Strnad, M.
AU  - Stamatovic, D.
AU  - Tukic, L.
AU  - Krtolica-Žikić, Koviljka
AU  - Tatomirovic, Z.
AU  - Đorđević, Vesna R.
AU  - Bogdanović, A.
AU  - Janković, G.
AU  - Magic, Z.
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4272
AB  - Purpose: The aim of this study was to analyze the occurrence of the most frequent BCR-ABL transcript variants (b3a2, b2a2 and ela2) in Serbian patients with chronic myeloid leukemia (CML) and compare it with the occurrence reported in other populations. Methods: We analyzed peripheral blood and bone marrow samples of 136 Serbian patients with CML by RT-PCR and cytogenetic methods. Results: In 100 patients (73.5%) the b3a2 and in 34 (25%) the b2a2 forms of BCR-ABL were detected. One (0.75%) patient was BCR-ABL negative, but in lymphoblastic transformation he expressed the ela1 transcript of BCR-ABL. One (0.75%) patient displayed both b2a2 and b3a2 forms of BCR-ABL. Analysis of this group according to karyotype showed b3a2 predominance (79%) in patients with classic t(9;22); b2a2 was found in 20% and both b2a2 and b3a2 forms in 1%. In variant translocations b3a2 in 65% and b2a2 in 35% of the patients were detected. In contrast, the subgroup with normal karyotype expressed slight predominance of the b2a2 form (50%); b3a2 was found in 43% of the patients and one patient (7%) displayed ela2. Conclusion: Predominance of the b3a2 form in Serbian patients with CML is in concordance with other relevant investigations, conducted mostly on Caucasian ethnic groups, but in contrast to the study performed on the Mestizo ethnic group in Ecuador Slight predominance of the b2a2 form was also noticed among the patients with normal karyotype.
T2  - Journal of BUON
T1  - Frequency of BCR-ABL fusion transcripts in Serbian patients with chronic myeloid leukemia
VL  - 16
IS  - 1
SP  - 104
EP  - 107
UR  - https://hdl.handle.net/21.15107/rcub_vinar_4272
ER  - 

@article{
author = "Todorić-Živanović, B. and Strnad, M. and Stamatovic, D. and Tukic, L. and Krtolica-Žikić, Koviljka and Tatomirovic, Z. and Đorđević, Vesna R. and Bogdanović, A. and Janković, G. and Magic, Z.",
year = "2011",
abstract = "Purpose: The aim of this study was to analyze the occurrence of the most frequent BCR-ABL transcript variants (b3a2, b2a2 and ela2) in Serbian patients with chronic myeloid leukemia (CML) and compare it with the occurrence reported in other populations. Methods: We analyzed peripheral blood and bone marrow samples of 136 Serbian patients with CML by RT-PCR and cytogenetic methods. Results: In 100 patients (73.5%) the b3a2 and in 34 (25%) the b2a2 forms of BCR-ABL were detected. One (0.75%) patient was BCR-ABL negative, but in lymphoblastic transformation he expressed the ela1 transcript of BCR-ABL. One (0.75%) patient displayed both b2a2 and b3a2 forms of BCR-ABL. Analysis of this group according to karyotype showed b3a2 predominance (79%) in patients with classic t(9;22); b2a2 was found in 20% and both b2a2 and b3a2 forms in 1%. In variant translocations b3a2 in 65% and b2a2 in 35% of the patients were detected. In contrast, the subgroup with normal karyotype expressed slight predominance of the b2a2 form (50%); b3a2 was found in 43% of the patients and one patient (7%) displayed ela2. Conclusion: Predominance of the b3a2 form in Serbian patients with CML is in concordance with other relevant investigations, conducted mostly on Caucasian ethnic groups, but in contrast to the study performed on the Mestizo ethnic group in Ecuador Slight predominance of the b2a2 form was also noticed among the patients with normal karyotype.",
journal = "Journal of BUON",
title = "Frequency of BCR-ABL fusion transcripts in Serbian patients with chronic myeloid leukemia",
volume = "16",
number = "1",
pages = "104-107",
url = "https://hdl.handle.net/21.15107/rcub_vinar_4272"
}

Todorić-Živanović, B., Strnad, M., Stamatovic, D., Tukic, L., Krtolica-Žikić, K., Tatomirovic, Z., Đorđević, V. R., Bogdanović, A., Janković, G.,& Magic, Z.. (2011). Frequency of BCR-ABL fusion transcripts in Serbian patients with chronic myeloid leukemia. in Journal of BUON, 16(1), 104-107.
https://hdl.handle.net/21.15107/rcub_vinar_4272

Todorić-Živanović B, Strnad M, Stamatovic D, Tukic L, Krtolica-Žikić K, Tatomirovic Z, Đorđević VR, Bogdanović A, Janković G, Magic Z. Frequency of BCR-ABL fusion transcripts in Serbian patients with chronic myeloid leukemia. in Journal of BUON. 2011;16(1):104-107.
https://hdl.handle.net/21.15107/rcub_vinar_4272 .

Todorić-Živanović, B., Strnad, M., Stamatovic, D., Tukic, L., Krtolica-Žikić, Koviljka, Tatomirovic, Z., Đorđević, Vesna R., Bogdanović, A., Janković, G., Magic, Z., "Frequency of BCR-ABL fusion transcripts in Serbian patients with chronic myeloid leukemia" in Journal of BUON, 16, no. 1 (2011):104-107,
https://hdl.handle.net/21.15107/rcub_vinar_4272 .