TY  - JOUR
AU  - Stojanović, Gordana
AU  - Stanković, Miroslava
AU  - Stojanovic, Igor Z.
AU  - Palic, Ivan
AU  - Milovanović, Vesna
AU  - Rancic, Sofija
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5969
AB  - Three lichen secondary metabolites atranorin (1), evernic acid (2), and usnic acid (3), were evaluated for their in vitro clastogenic and antiproliferative effects on human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay at concentrations of 2 mu g/mL, 4 mu g/mL and 6 mu g/mL of final culture solution. The frequency of micronucleus (MN) was scored in binucleated cells, and cytokinesis-block proliferation index (CBPI) was calculated. Among the tested compounds, 3 exhibited the most prominent effect decreasing the frequency of MN in the range of 42.5% - 48.9%, that is about double of the positive control amifostin WR-2721 that reduces MN frequency for 22.0%. The effect of evernic acid was approximately equal to action of amifostin (23.2% -32.9%). Atranorin at concentrations of 2 mu g/mL and 4 mu g/mL decreasing the frequency of MN only for 11.1% and 1.8%, while in concentration of 6 mu g/mL increases the frequency of MN for 9.6 %. The comparable CBPI values of the investigated compounds and control suggested that they did not show a statistically significant inhibitory effect on lymphocyte cell proliferation at applied concentrations.
T2  - Natural Product Communications
T1  - Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes
VL  - 9
IS  - 4
SP  - 503
EP  - 504
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5969
ER  - 

@article{
author = "Stojanović, Gordana and Stanković, Miroslava and Stojanovic, Igor Z. and Palic, Ivan and Milovanović, Vesna and Rancic, Sofija",
year = "2014",
abstract = "Three lichen secondary metabolites atranorin (1), evernic acid (2), and usnic acid (3), were evaluated for their in vitro clastogenic and antiproliferative effects on human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay at concentrations of 2 mu g/mL, 4 mu g/mL and 6 mu g/mL of final culture solution. The frequency of micronucleus (MN) was scored in binucleated cells, and cytokinesis-block proliferation index (CBPI) was calculated. Among the tested compounds, 3 exhibited the most prominent effect decreasing the frequency of MN in the range of 42.5% - 48.9%, that is about double of the positive control amifostin WR-2721 that reduces MN frequency for 22.0%. The effect of evernic acid was approximately equal to action of amifostin (23.2% -32.9%). Atranorin at concentrations of 2 mu g/mL and 4 mu g/mL decreasing the frequency of MN only for 11.1% and 1.8%, while in concentration of 6 mu g/mL increases the frequency of MN for 9.6 %. The comparable CBPI values of the investigated compounds and control suggested that they did not show a statistically significant inhibitory effect on lymphocyte cell proliferation at applied concentrations.",
journal = "Natural Product Communications",
title = "Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes",
volume = "9",
number = "4",
pages = "503-504",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5969"
}

Stojanović, G., Stanković, M., Stojanovic, I. Z., Palic, I., Milovanović, V.,& Rancic, S.. (2014). Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes. in Natural Product Communications, 9(4), 503-504.
https://hdl.handle.net/21.15107/rcub_vinar_5969

Stojanović G, Stanković M, Stojanovic IZ, Palic I, Milovanović V, Rancic S. Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes. in Natural Product Communications. 2014;9(4):503-504.
https://hdl.handle.net/21.15107/rcub_vinar_5969 .

Stojanović, Gordana, Stanković, Miroslava, Stojanovic, Igor Z., Palic, Ivan, Milovanović, Vesna, Rancic, Sofija, "Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes" in Natural Product Communications, 9, no. 4 (2014):503-504,
https://hdl.handle.net/21.15107/rcub_vinar_5969 .

TY  - JOUR
AU  - Stojanovic, Igor Z.
AU  - Stanković, Miroslava
AU  - Jovanovic, Olga
AU  - Petrovic, Goran
AU  - Smelcerovic, Andrija
AU  - Stojanović, Gordana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5302
AB  - Three lichen depsidones, physodalic acid (1), physodic acid (2), and 3-hydroxy physodic acid (3), were isolated from Hypogymnia physodes diethyl ether extract using column chromatography, and their structures determined by comparing their UV, H-1 and C-13 NMR spectroscopic and MS data with those given in the literature, as well as with data computed by CHEM draw ultra 11 software. The contents of 1, 2 and 3 were determined in the methanol (ME), acetone (AE), and diethyl ether (EE) extracts using reversed-phase high performance liquid chromatography with photodiode array detection. The extracts, isolated depsidones 1-3 and EE fraction F23 (consisting of 90% 2 and 3, in the ratio 5.5: 1) were evaluated for their in vitro effects on chromosome aberrations in peripheral human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay in doses of 1 mu g/mL and 2 mu g/mL of final culture solution. The frequency of MN was scored in binucleated cells, and nuclear proliferation index was calculated. It was found that 1, 2, 3, F23, and EE at 1.0 mu g/mL exerted a beneficial effect on lymphocyte cells giving a significant decrease of the frequency of MN in comparison with the positive control Amifostin WR-2721. Among the tested depsidones at a concentration of 1 mu g/mL, 3 exhibited the most prominent effect decreasing the frequency of MN by 30.3%, followed by 2 (28.2%) and 1 (22.0%). The extracts were less effective than the isolated depsidones.
T2  - Natural Product Communications
T1  - Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes
VL  - 8
IS  - 1
SP  - 109
EP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5302
ER  - 

@article{
author = "Stojanovic, Igor Z. and Stanković, Miroslava and Jovanovic, Olga and Petrovic, Goran and Smelcerovic, Andrija and Stojanović, Gordana",
year = "2013",
abstract = "Three lichen depsidones, physodalic acid (1), physodic acid (2), and 3-hydroxy physodic acid (3), were isolated from Hypogymnia physodes diethyl ether extract using column chromatography, and their structures determined by comparing their UV, H-1 and C-13 NMR spectroscopic and MS data with those given in the literature, as well as with data computed by CHEM draw ultra 11 software. The contents of 1, 2 and 3 were determined in the methanol (ME), acetone (AE), and diethyl ether (EE) extracts using reversed-phase high performance liquid chromatography with photodiode array detection. The extracts, isolated depsidones 1-3 and EE fraction F23 (consisting of 90% 2 and 3, in the ratio 5.5: 1) were evaluated for their in vitro effects on chromosome aberrations in peripheral human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay in doses of 1 mu g/mL and 2 mu g/mL of final culture solution. The frequency of MN was scored in binucleated cells, and nuclear proliferation index was calculated. It was found that 1, 2, 3, F23, and EE at 1.0 mu g/mL exerted a beneficial effect on lymphocyte cells giving a significant decrease of the frequency of MN in comparison with the positive control Amifostin WR-2721. Among the tested depsidones at a concentration of 1 mu g/mL, 3 exhibited the most prominent effect decreasing the frequency of MN by 30.3%, followed by 2 (28.2%) and 1 (22.0%). The extracts were less effective than the isolated depsidones.",
journal = "Natural Product Communications",
title = "Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes",
volume = "8",
number = "1",
pages = "109-112",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5302"
}

Stojanovic, I. Z., Stanković, M., Jovanovic, O., Petrovic, G., Smelcerovic, A.,& Stojanović, G.. (2013). Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes. in Natural Product Communications, 8(1), 109-112.
https://hdl.handle.net/21.15107/rcub_vinar_5302

Stojanovic IZ, Stanković M, Jovanovic O, Petrovic G, Smelcerovic A, Stojanović G. Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes. in Natural Product Communications. 2013;8(1):109-112.
https://hdl.handle.net/21.15107/rcub_vinar_5302 .

Stojanovic, Igor Z., Stanković, Miroslava, Jovanovic, Olga, Petrovic, Goran, Smelcerovic, Andrija, Stojanović, Gordana, "Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes" in Natural Product Communications, 8, no. 1 (2013):109-112,
https://hdl.handle.net/21.15107/rcub_vinar_5302 .