TY  - CONF
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad L.
AU  - Petrović, Nenad
AU  - Rajković, Jelena
AU  - Kenić Marinković, Dragana
AU  - Najman, Stevo
AU  - Mihailović, Dragan
AU  - Uskoković, Dragan
PY  - 2014
UR  - http://dais.sanu.ac.rs/123456789/558
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7477
AB  - Recent studies have shown that the behavior of nanoparticles in in vivo conditions is not solely dependant on their physical properties, such are size and shape. The surface of nanoparticles in biological fluids interacts with biomolecules such are proteins, adsorbs these molecules, and leads to formation of nanoparticle-biomoelcular complex known as “protein corona”. This protein corona changes the properties of nanoparticles and their behavior in vivo. In this work three types of nanomaterial based on Hap and polymers were incubated in rats plasma, and the identification and quantification of proteins in protein corona that had formed around these nanoparticles, was done by means of electrophoresis and mass spectrophotometery.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts
T1  - Interaction of nanoparticles and biological fluids
SP  - 113
EP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7477
ER  - 

@conference{
author = "Ajduković, Zorica and Ignjatović, Nenad L. and Petrović, Nenad and Rajković, Jelena and Kenić Marinković, Dragana and Najman, Stevo and Mihailović, Dragan and Uskoković, Dragan",
year = "2014",
abstract = "Recent studies have shown that the behavior of nanoparticles in in vivo conditions is not solely dependant on their physical properties, such are size and shape. The surface of nanoparticles in biological fluids interacts with biomolecules such are proteins, adsorbs these molecules, and leads to formation of nanoparticle-biomoelcular complex known as “protein corona”. This protein corona changes the properties of nanoparticles and their behavior in vivo. In this work three types of nanomaterial based on Hap and polymers were incubated in rats plasma, and the identification and quantification of proteins in protein corona that had formed around these nanoparticles, was done by means of electrophoresis and mass spectrophotometery.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts",
title = "Interaction of nanoparticles and biological fluids",
pages = "113-113",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7477"
}

Ajduković, Z., Ignjatović, N. L., Petrović, N., Rajković, J., Kenić Marinković, D., Najman, S., Mihailović, D.,& Uskoković, D.. (2014). Interaction of nanoparticles and biological fluids. in The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 113-113.
https://hdl.handle.net/21.15107/rcub_vinar_7477

Ajduković Z, Ignjatović NL, Petrović N, Rajković J, Kenić Marinković D, Najman S, Mihailović D, Uskoković D. Interaction of nanoparticles and biological fluids. in The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts. 2014;:113-113.
https://hdl.handle.net/21.15107/rcub_vinar_7477 .

Ajduković, Zorica, Ignjatović, Nenad L., Petrović, Nenad, Rajković, Jelena, Kenić Marinković, Dragana, Najman, Stevo, Mihailović, Dragan, Uskoković, Dragan, "Interaction of nanoparticles and biological fluids" in The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts (2014):113-113,
https://hdl.handle.net/21.15107/rcub_vinar_7477 .

TY  - JOUR
AU  - Ignjatović, Nenad L.
AU  - Ajduković, Zorica
AU  - Savić, Vojin
AU  - Najman, Stevo
AU  - Mihailović, Dragan
AU  - Vasiljević, Perica
AU  - Stojanović, Zoran S.
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7410
AB  - Indications exist that paramagnetic calcium phosphates may be able to promote regeneration of bone faster than their regular, diamagnetic counterparts. In this study, analyzed was the influence of paramagnetic cobalt-substituted hydroxyapatite nanoparticles on osteoporotic alveolar bone regeneration in rats. Simultaneously, biocompatibility of the material was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. The material was shown to be biocompatible and nontoxic when added to epithelial monolayers in vitro, while it caused a substantial decrease in the cell viability as well as deformation of the cytoskeleton and cell morphology when incubated with the osteoblastic cells. In the course of 6 months after the implantation of the material containing different amounts of cobalt, ranging from 5 to 12 wt%, in the osteoporotic alveolar bone of the lower jaw, the following parameters were investigated: histopathological parameters, alkaline phosphatase and alveolar bone density. The best result in terms of osteoporotic bone tissue regeneration was observed for hydroxyapatite nanoparticles with the largest content of cobalt ions. The histological analysis showed a high level of reparatory ability of the nanoparticulate material implanted in the bone defect, paralleled by a corresponding increase in the alveolar bone density. The combined effect of growth factors from autologous plasma admixed to cobalt-substituted hydroxyapatite was furthermore shown to have a crucial effect on the augmented osteoporotic bone regeneration upon the implantation of the biomaterial investigated in this study.
T2  - Journal of Materials Science: Materials in Medicine
T1  - Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones
SP  - 343
EP  - 354
DO  - 10.1007/s10856-012-4793-1
ER  - 

@article{
author = "Ignjatović, Nenad L. and Ajduković, Zorica and Savić, Vojin and Najman, Stevo and Mihailović, Dragan and Vasiljević, Perica and Stojanović, Zoran S. and Uskoković, Vuk and Uskoković, Dragan",
year = "2013",
abstract = "Indications exist that paramagnetic calcium phosphates may be able to promote regeneration of bone faster than their regular, diamagnetic counterparts. In this study, analyzed was the influence of paramagnetic cobalt-substituted hydroxyapatite nanoparticles on osteoporotic alveolar bone regeneration in rats. Simultaneously, biocompatibility of the material was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. The material was shown to be biocompatible and nontoxic when added to epithelial monolayers in vitro, while it caused a substantial decrease in the cell viability as well as deformation of the cytoskeleton and cell morphology when incubated with the osteoblastic cells. In the course of 6 months after the implantation of the material containing different amounts of cobalt, ranging from 5 to 12 wt%, in the osteoporotic alveolar bone of the lower jaw, the following parameters were investigated: histopathological parameters, alkaline phosphatase and alveolar bone density. The best result in terms of osteoporotic bone tissue regeneration was observed for hydroxyapatite nanoparticles with the largest content of cobalt ions. The histological analysis showed a high level of reparatory ability of the nanoparticulate material implanted in the bone defect, paralleled by a corresponding increase in the alveolar bone density. The combined effect of growth factors from autologous plasma admixed to cobalt-substituted hydroxyapatite was furthermore shown to have a crucial effect on the augmented osteoporotic bone regeneration upon the implantation of the biomaterial investigated in this study.",
journal = "Journal of Materials Science: Materials in Medicine",
title = "Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones",
pages = "343-354",
doi = "10.1007/s10856-012-4793-1"
}

Ignjatović, N. L., Ajduković, Z., Savić, V., Najman, S., Mihailović, D., Vasiljević, P., Stojanović, Z. S., Uskoković, V.,& Uskoković, D.. (2013). Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones. in Journal of Materials Science: Materials in Medicine, 343-354.
https://doi.org/10.1007/s10856-012-4793-1

Ignjatović NL, Ajduković Z, Savić V, Najman S, Mihailović D, Vasiljević P, Stojanović ZS, Uskoković V, Uskoković D. Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones. in Journal of Materials Science: Materials in Medicine. 2013;:343-354.
doi:10.1007/s10856-012-4793-1 .

Ignjatović, Nenad L., Ajduković, Zorica, Savić, Vojin, Najman, Stevo, Mihailović, Dragan, Vasiljević, Perica, Stojanović, Zoran S., Uskoković, Vuk, Uskoković, Dragan, "Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones" in Journal of Materials Science: Materials in Medicine (2013):343-354,
https://doi.org/10.1007/s10856-012-4793-1 . .

TY  - JOUR
AU  - Ignjatović, Nenad L.
AU  - Ajduković, Zorica
AU  - Savić, Vojin
AU  - Najman, Stevo
AU  - Mihailović, Dragan
AU  - Vasiljević, Perica
AU  - Stojanović, Zoran S.
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7481
AB  - Indications exist that paramagnetic calcium phosphates may be able to promote regeneration of bone faster than their regular, diamagnetic counterparts. In this study, analyzed was the influence of paramagnetic cobalt-substituted hydroxyapatite nanoparticles on osteoporotic alveolar bone regeneration in rats. Simultaneously, biocompatibility of the material was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. The material was shown to be biocompatible and nontoxic when added to epithelial monolayers in vitro, while it caused a substantial decrease in the cell viability as well as deformation of the cytoskeleton and cell morphology when incubated with the osteoblastic cells. In the course of 6 months after the implantation of the material containing different amounts of cobalt, ranging from 5 to 12 wt%, in the osteoporotic alveolar bone of the lower jaw, the following parameters were investigated: histopathological parameters, alkaline phosphatase and alveolar bone density. The best result in terms of osteoporotic bone tissue regeneration was observed for hydroxyapatite nanoparticles with the largest content of cobalt ions. The histological analysis showed a high level of reparatory ability of the nanoparticulate material implanted in the bone defect, paralleled by a corresponding increase in the alveolar bone density. The combined effect of growth factors from autologous plasma admixed to cobalt-substituted hydroxyapatite was furthermore shown to have a crucial effect on the augmented osteoporotic bone regeneration upon the implantation of the biomaterial investigated in this study.
T2  - Journal of Materials Science: Materials in Medicine
T1  - Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones
SP  - 343
EP  - 354
DO  - 10.1007/s10856-012-4793-1
ER  - 

@article{
author = "Ignjatović, Nenad L. and Ajduković, Zorica and Savić, Vojin and Najman, Stevo and Mihailović, Dragan and Vasiljević, Perica and Stojanović, Zoran S. and Uskoković, Vuk and Uskoković, Dragan",
year = "2013",
abstract = "Indications exist that paramagnetic calcium phosphates may be able to promote regeneration of bone faster than their regular, diamagnetic counterparts. In this study, analyzed was the influence of paramagnetic cobalt-substituted hydroxyapatite nanoparticles on osteoporotic alveolar bone regeneration in rats. Simultaneously, biocompatibility of the material was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. The material was shown to be biocompatible and nontoxic when added to epithelial monolayers in vitro, while it caused a substantial decrease in the cell viability as well as deformation of the cytoskeleton and cell morphology when incubated with the osteoblastic cells. In the course of 6 months after the implantation of the material containing different amounts of cobalt, ranging from 5 to 12 wt%, in the osteoporotic alveolar bone of the lower jaw, the following parameters were investigated: histopathological parameters, alkaline phosphatase and alveolar bone density. The best result in terms of osteoporotic bone tissue regeneration was observed for hydroxyapatite nanoparticles with the largest content of cobalt ions. The histological analysis showed a high level of reparatory ability of the nanoparticulate material implanted in the bone defect, paralleled by a corresponding increase in the alveolar bone density. The combined effect of growth factors from autologous plasma admixed to cobalt-substituted hydroxyapatite was furthermore shown to have a crucial effect on the augmented osteoporotic bone regeneration upon the implantation of the biomaterial investigated in this study.",
journal = "Journal of Materials Science: Materials in Medicine",
title = "Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones",
pages = "343-354",
doi = "10.1007/s10856-012-4793-1"
}

Ignjatović, N. L., Ajduković, Z., Savić, V., Najman, S., Mihailović, D., Vasiljević, P., Stojanović, Z. S., Uskoković, V.,& Uskoković, D.. (2013). Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones. in Journal of Materials Science: Materials in Medicine, 343-354.
https://doi.org/10.1007/s10856-012-4793-1

Ignjatović NL, Ajduković Z, Savić V, Najman S, Mihailović D, Vasiljević P, Stojanović ZS, Uskoković V, Uskoković D. Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones. in Journal of Materials Science: Materials in Medicine. 2013;:343-354.
doi:10.1007/s10856-012-4793-1 .

Ignjatović, Nenad L., Ajduković, Zorica, Savić, Vojin, Najman, Stevo, Mihailović, Dragan, Vasiljević, Perica, Stojanović, Zoran S., Uskoković, Vuk, Uskoković, Dragan, "Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones" in Journal of Materials Science: Materials in Medicine (2013):343-354,
https://doi.org/10.1007/s10856-012-4793-1 . .

TY  - CONF
AU  - Ajduković, Zorica
AU  - Petrović, Milica B.
AU  - Milićević, J.
AU  - Đorđević, N.
AU  - Ignjatović, Nenad L.
AU  - Savić, Vojin
AU  - Najman, Stevo
AU  - Mihailović, Dragan
AU  - Vasiljević, P.
AU  - Uskoković, Dragan
PY  - 2011
UR  - http://dais.sanu.ac.rs/123456789/674
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7505
AB  - One of the trends in the treatment of reduced bone density is in oral implantology through the use of new biomaterials with paramagnetic properties. In this in vivo study the effect of Ca /Co-HAp nanoparticles (calcium/cobalt-hydroxiapatite) in the regeneration of the reduced alveolar bone density in experimental animals was examined by analyzing both the biochemical and histochemical markers and through analysis of alveolar bone density. The research was carried out on female Westar rats, aged 6-8 weeks, whom have been implanted nanobiomaterial in reduced alveolar bone density. Good results in regeneration of reduced bone density were achieved in six weeks after implantation of the nanoparticles with paramagnetic properties. Biochemical markers of osteogenesis showed statistically significant rise. Histological analysis revealed high level reparatory skills of the biocomposite implanted in the bone defect, while X-ray analyses evidenced rise of bone density. Implantation of Ca/Co-Hap enables rapid new formation, thus becoming the material of choice for accelerated reduced bone density regeneration.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts
T1  - Paramagnetic nanoparticles in the regeneration of the reduced alveolar bone density
SP  - 161
EP  - 161
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7505
ER  - 

@conference{
author = "Ajduković, Zorica and Petrović, Milica B. and Milićević, J. and Đorđević, N. and Ignjatović, Nenad L. and Savić, Vojin and Najman, Stevo and Mihailović, Dragan and Vasiljević, P. and Uskoković, Dragan",
year = "2011",
abstract = "One of the trends in the treatment of reduced bone density is in oral implantology through the use of new biomaterials with paramagnetic properties. In this in vivo study the effect of Ca /Co-HAp nanoparticles (calcium/cobalt-hydroxiapatite) in the regeneration of the reduced alveolar bone density in experimental animals was examined by analyzing both the biochemical and histochemical markers and through analysis of alveolar bone density. The research was carried out on female Westar rats, aged 6-8 weeks, whom have been implanted nanobiomaterial in reduced alveolar bone density. Good results in regeneration of reduced bone density were achieved in six weeks after implantation of the nanoparticles with paramagnetic properties. Biochemical markers of osteogenesis showed statistically significant rise. Histological analysis revealed high level reparatory skills of the biocomposite implanted in the bone defect, while X-ray analyses evidenced rise of bone density. Implantation of Ca/Co-Hap enables rapid new formation, thus becoming the material of choice for accelerated reduced bone density regeneration.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts",
title = "Paramagnetic nanoparticles in the regeneration of the reduced alveolar bone density",
pages = "161-161",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7505"
}

Ajduković, Z., Petrović, M. B., Milićević, J., Đorđević, N., Ignjatović, N. L., Savić, V., Najman, S., Mihailović, D., Vasiljević, P.,& Uskoković, D.. (2011). Paramagnetic nanoparticles in the regeneration of the reduced alveolar bone density. in The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 161-161.
https://hdl.handle.net/21.15107/rcub_vinar_7505

Ajduković Z, Petrović MB, Milićević J, Đorđević N, Ignjatović NL, Savić V, Najman S, Mihailović D, Vasiljević P, Uskoković D. Paramagnetic nanoparticles in the regeneration of the reduced alveolar bone density. in The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts. 2011;:161-161.
https://hdl.handle.net/21.15107/rcub_vinar_7505 .

Ajduković, Zorica, Petrović, Milica B., Milićević, J., Đorđević, N., Ignjatović, Nenad L., Savić, Vojin, Najman, Stevo, Mihailović, Dragan, Vasiljević, P., Uskoković, Dragan, "Paramagnetic nanoparticles in the regeneration of the reduced alveolar bone density" in The Thirteenth Annual Conference YUCOMAT 2011: Programme and the Book of Abstracts (2011):161-161,
https://hdl.handle.net/21.15107/rcub_vinar_7505 .

TY  - CONF
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad L.
AU  - Petrović, Nenad
AU  - Savić, Vojin
AU  - Mihailović, Dragan
AU  - Uskoković, Dragan
PY  - 2011
UR  - http://dais.sanu.ac.rs/123456789/690
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7512
AB  - Systems for targeted delivery of drugs with high affinity for specific organs, tissues, and cells were introduced at the beginning of the twentieth century. Recently, this concept is attracting much attention. These systems have shown most effective in reducing adverse effects of drugs. In recent years, scientists have created a system of nanoparticles for delivery of vitamin D. Special attention is paid to the role of vitamin D in the division and differentiation of osteoblasts and thus, in its role in osteogenesis. In this regard it was interesting to observe the local effects of the active form of vitamin D3 in osteogenesis, as a part of the system for local delivery. These effects were observed on an experimental model. Three types of nanobiomaterial were implanted in the artificially made defects in rats’ mandibular bone. Six weeks after implantation of hydroxyapatite, hydroxyapatite-coated vitamin D3 and hydroxyapatite nanoparticles coated with vitamin D3 mixed with the polymer, the animals were sacrificed, and the samples of mandibular alveolar bone were taken. The samples were adequately prepared and subjected to histopathological analysis. The best results in regeneration of osteoporotic jaw bone were obtained in the experimental group of animals where the implant was hydroxyapatite-coated vitamin D3 mixed with the polymer. The results show that nanobiomaterials with local delivery of active form of vitamin D3, used in this study, may be materials of the future in the area of weakened jaw bone tissue regeneration.
PB  - Belgrade : Institute of Technical Sciences of the Serbian Academy of Sciences and Arts
C3  - Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia
T1  - Joint effects of vitamin D and nanobiomaterial in the jaw bone regeneration
SP  - 9
EP  - 9
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7512
ER  - 

@conference{
author = "Ajduković, Zorica and Ignjatović, Nenad L. and Petrović, Nenad and Savić, Vojin and Mihailović, Dragan and Uskoković, Dragan",
year = "2011",
abstract = "Systems for targeted delivery of drugs with high affinity for specific organs, tissues, and cells were introduced at the beginning of the twentieth century. Recently, this concept is attracting much attention. These systems have shown most effective in reducing adverse effects of drugs. In recent years, scientists have created a system of nanoparticles for delivery of vitamin D. Special attention is paid to the role of vitamin D in the division and differentiation of osteoblasts and thus, in its role in osteogenesis. In this regard it was interesting to observe the local effects of the active form of vitamin D3 in osteogenesis, as a part of the system for local delivery. These effects were observed on an experimental model. Three types of nanobiomaterial were implanted in the artificially made defects in rats’ mandibular bone. Six weeks after implantation of hydroxyapatite, hydroxyapatite-coated vitamin D3 and hydroxyapatite nanoparticles coated with vitamin D3 mixed with the polymer, the animals were sacrificed, and the samples of mandibular alveolar bone were taken. The samples were adequately prepared and subjected to histopathological analysis. The best results in regeneration of osteoporotic jaw bone were obtained in the experimental group of animals where the implant was hydroxyapatite-coated vitamin D3 mixed with the polymer. The results show that nanobiomaterials with local delivery of active form of vitamin D3, used in this study, may be materials of the future in the area of weakened jaw bone tissue regeneration.",
publisher = "Belgrade : Institute of Technical Sciences of the Serbian Academy of Sciences and Arts",
journal = "Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia",
title = "Joint effects of vitamin D and nanobiomaterial in the jaw bone regeneration",
pages = "9-9",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7512"
}

Ajduković, Z., Ignjatović, N. L., Petrović, N., Savić, V., Mihailović, D.,& Uskoković, D.. (2011). Joint effects of vitamin D and nanobiomaterial in the jaw bone regeneration. in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of the Serbian Academy of Sciences and Arts., 9-9.
https://hdl.handle.net/21.15107/rcub_vinar_7512

Ajduković Z, Ignjatović NL, Petrović N, Savić V, Mihailović D, Uskoković D. Joint effects of vitamin D and nanobiomaterial in the jaw bone regeneration. in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia. 2011;:9-9.
https://hdl.handle.net/21.15107/rcub_vinar_7512 .

Ajduković, Zorica, Ignjatović, Nenad L., Petrović, Nenad, Savić, Vojin, Mihailović, Dragan, Uskoković, Dragan, "Joint effects of vitamin D and nanobiomaterial in the jaw bone regeneration" in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia (2011):9-9,
https://hdl.handle.net/21.15107/rcub_vinar_7512 .