Demajo, Miroslav

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orcid::0000-0003-3514-7902
  • Demajo, Miroslav (39)

Author's Bibliography

Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach

Joksić, Gordana; Mićić, Mileva; Filipović, Jelena G.; Drakulić, Dunja R.; Stanojlović, Miloš R.; Calija, Bojan; Valenta-Šobot, Ana; Demajo, Miroslav; Nilsson, Robert

(2017)

TY  - JOUR
AU  - Joksić, Gordana
AU  - Mićić, Mileva
AU  - Filipović, Jelena G.
AU  - Drakulić, Dunja R.
AU  - Stanojlović, Miloš R.
AU  - Calija, Bojan
AU  - Valenta-Šobot, Ana
AU  - Demajo, Miroslav
AU  - Nilsson, Robert
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1584
AB  - The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.
T2  - Acta Veterinaria, Beograd
T1  - Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach
VL  - 67
IS  - 1
SP  - 1
EP  - 10
DO  - 10.1515/acve-2017-0001
ER  - 
@article{
author = "Joksić, Gordana and Mićić, Mileva and Filipović, Jelena G. and Drakulić, Dunja R. and Stanojlović, Miloš R. and Calija, Bojan and Valenta-Šobot, Ana and Demajo, Miroslav and Nilsson, Robert",
year = "2017",
abstract = "The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.",
journal = "Acta Veterinaria, Beograd",
title = "Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach",
volume = "67",
number = "1",
pages = "1-10",
doi = "10.1515/acve-2017-0001"
}
Joksić, G., Mićić, M., Filipović, J. G., Drakulić, D. R., Stanojlović, M. R., Calija, B., Valenta-Šobot, A., Demajo, M.,& Nilsson, R.. (2017). Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach. in Acta Veterinaria, Beograd, 67(1), 1-10.
https://doi.org/10.1515/acve-2017-0001
Joksić G, Mićić M, Filipović JG, Drakulić DR, Stanojlović MR, Calija B, Valenta-Šobot A, Demajo M, Nilsson R. Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach. in Acta Veterinaria, Beograd. 2017;67(1):1-10.
doi:10.1515/acve-2017-0001 .
Joksić, Gordana, Mićić, Mileva, Filipović, Jelena G., Drakulić, Dunja R., Stanojlović, Miloš R., Calija, Bojan, Valenta-Šobot, Ana, Demajo, Miroslav, Nilsson, Robert, "Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach" in Acta Veterinaria, Beograd, 67, no. 1 (2017):1-10,
https://doi.org/10.1515/acve-2017-0001 . .
1
1

Case with triple-negative breast cancer shows overexpression of both cFOS and TGF-beta 1 in node-positive tissue

Ivanović, Vesna; Dedović-Tanić, Nasta; Milovanović, Zorka M.; Lukić, Silvana; Nikolic, Srdjan; Baltic, Vladimir; Stojiljković, Bratislav; Demajo, Miroslav; Mandušić, Vesna; Dimitrijević, Bogomir B.

(2016)

TY  - JOUR
AU  - Ivanović, Vesna
AU  - Dedović-Tanić, Nasta
AU  - Milovanović, Zorka M.
AU  - Lukić, Silvana
AU  - Nikolic, Srdjan
AU  - Baltic, Vladimir
AU  - Stojiljković, Bratislav
AU  - Demajo, Miroslav
AU  - Mandušić, Vesna
AU  - Dimitrijević, Bogomir B.
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1327
AB  - We present herein a case report style article on a rare advanced triple-negative breast cancer (TNBC) patient with 6-month disease-free interval, and 10-month overall survival. Our results demonstrate that the poor clinical outcome of this patient was associated with pronounced, more than fivefold higher, overexpression of both cFOS and TGF-beta 1 proteins in its metastatic nodal tissue extracts, when compared with the values of the two non-TNBC controls (with zero disease-free interval and overall survival). This original observation suggests, for the first time, that both the cFOS and TGF-beta 1 may be considered as a pair of biomarkers for an early assessment of poor prognosis for TNBC patients. The possible clinical implication of this observation is discussed.
T2  - Personalized Medicine
T1  - Case with triple-negative breast cancer shows overexpression of both cFOS and TGF-beta 1 in node-positive tissue
VL  - 13
IS  - 6
SP  - 523
EP  - 530
DO  - 10.2217/pme-2016-0032
ER  - 
@article{
author = "Ivanović, Vesna and Dedović-Tanić, Nasta and Milovanović, Zorka M. and Lukić, Silvana and Nikolic, Srdjan and Baltic, Vladimir and Stojiljković, Bratislav and Demajo, Miroslav and Mandušić, Vesna and Dimitrijević, Bogomir B.",
year = "2016",
abstract = "We present herein a case report style article on a rare advanced triple-negative breast cancer (TNBC) patient with 6-month disease-free interval, and 10-month overall survival. Our results demonstrate that the poor clinical outcome of this patient was associated with pronounced, more than fivefold higher, overexpression of both cFOS and TGF-beta 1 proteins in its metastatic nodal tissue extracts, when compared with the values of the two non-TNBC controls (with zero disease-free interval and overall survival). This original observation suggests, for the first time, that both the cFOS and TGF-beta 1 may be considered as a pair of biomarkers for an early assessment of poor prognosis for TNBC patients. The possible clinical implication of this observation is discussed.",
journal = "Personalized Medicine",
title = "Case with triple-negative breast cancer shows overexpression of both cFOS and TGF-beta 1 in node-positive tissue",
volume = "13",
number = "6",
pages = "523-530",
doi = "10.2217/pme-2016-0032"
}
Ivanović, V., Dedović-Tanić, N., Milovanović, Z. M., Lukić, S., Nikolic, S., Baltic, V., Stojiljković, B., Demajo, M., Mandušić, V.,& Dimitrijević, B. B.. (2016). Case with triple-negative breast cancer shows overexpression of both cFOS and TGF-beta 1 in node-positive tissue. in Personalized Medicine, 13(6), 523-530.
https://doi.org/10.2217/pme-2016-0032
Ivanović V, Dedović-Tanić N, Milovanović ZM, Lukić S, Nikolic S, Baltic V, Stojiljković B, Demajo M, Mandušić V, Dimitrijević BB. Case with triple-negative breast cancer shows overexpression of both cFOS and TGF-beta 1 in node-positive tissue. in Personalized Medicine. 2016;13(6):523-530.
doi:10.2217/pme-2016-0032 .
Ivanović, Vesna, Dedović-Tanić, Nasta, Milovanović, Zorka M., Lukić, Silvana, Nikolic, Srdjan, Baltic, Vladimir, Stojiljković, Bratislav, Demajo, Miroslav, Mandušić, Vesna, Dimitrijević, Bogomir B., "Case with triple-negative breast cancer shows overexpression of both cFOS and TGF-beta 1 in node-positive tissue" in Personalized Medicine, 13, no. 6 (2016):523-530,
https://doi.org/10.2217/pme-2016-0032 . .
1
1
1
1

Different susceptibility of prefrontal cortex and hippocampus to oxidative stress following chronic social isolation stress

Martinović, Jelena; Todorović, Nevena; Bošković, Maja; Pajović, Snežana B.; Demajo, Miroslav; Filipović, Dragana

(2014)

TY  - JOUR
AU  - Martinović, Jelena
AU  - Todorović, Nevena
AU  - Bošković, Maja
AU  - Pajović, Snežana B.
AU  - Demajo, Miroslav
AU  - Filipović, Dragana
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6058
AB  - Chronic oxidative stress plays an important role in depression. The aim of present study was to examine the stress-induced changes in serum corticosterone (CORT) levels, cytosolic protein carbonyl groups, malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO) and total superoxide dismutase (SOD) activity in the prefrontal cortex versus hippocampus of male Wistar rats exposed to acute (2 h of immobilization or cold), chronic (21d of social isolation) stress, and their combination (chronic + acute stress). The subcellular distribution of nuclear factor-kappa B (NF-kappa B) and cytosolic cyclooxygenase 2 (COX-2) protein expressions were also examined. Depressive- and anxiety-like behaviors were assessed via the forced swim, sucrose preference, and marble burying tests in chronically isolated rats. Although both acute stressors resulted in elevated CORT, increased MDA in the prefrontal cortex and NF-kappa B activation accompanied by increased NO in the hippocampus were detected only following acute cold stress. Chronic isolation resulted in no change in CORT levels, but disabled appropriate response to novel acute stress and led to depressive- and anxiety-like behaviors. Increased oxidative/nitrosative stress markers, likely by NF-kappa B nuclear translocation and concomitant COX-2 upregulation, associated with decreased SOD activity and GSH levels, suggested the existence of oxidative stress in the prefrontal cortex. In contrast, hippocampus was less susceptible to oxidative damage showing only increase in protein carbonyl groups and depleted GSH. Taken together, the prefrontal cortex seems to be more sensitive to oxidative stress than the hippocampus following chronic isolation stress, which may be relevant for further research related to stress-induced depressive-like behavior.
T2  - Molecular and Cellular Biochemistry
T1  - Different susceptibility of prefrontal cortex and hippocampus to oxidative stress following chronic social isolation stress
VL  - 393
IS  - 1-2
SP  - 43
EP  - 57
DO  - 10.1007/s11010-014-2045-z
ER  - 
@article{
author = "Martinović, Jelena and Todorović, Nevena and Bošković, Maja and Pajović, Snežana B. and Demajo, Miroslav and Filipović, Dragana",
year = "2014",
abstract = "Chronic oxidative stress plays an important role in depression. The aim of present study was to examine the stress-induced changes in serum corticosterone (CORT) levels, cytosolic protein carbonyl groups, malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO) and total superoxide dismutase (SOD) activity in the prefrontal cortex versus hippocampus of male Wistar rats exposed to acute (2 h of immobilization or cold), chronic (21d of social isolation) stress, and their combination (chronic + acute stress). The subcellular distribution of nuclear factor-kappa B (NF-kappa B) and cytosolic cyclooxygenase 2 (COX-2) protein expressions were also examined. Depressive- and anxiety-like behaviors were assessed via the forced swim, sucrose preference, and marble burying tests in chronically isolated rats. Although both acute stressors resulted in elevated CORT, increased MDA in the prefrontal cortex and NF-kappa B activation accompanied by increased NO in the hippocampus were detected only following acute cold stress. Chronic isolation resulted in no change in CORT levels, but disabled appropriate response to novel acute stress and led to depressive- and anxiety-like behaviors. Increased oxidative/nitrosative stress markers, likely by NF-kappa B nuclear translocation and concomitant COX-2 upregulation, associated with decreased SOD activity and GSH levels, suggested the existence of oxidative stress in the prefrontal cortex. In contrast, hippocampus was less susceptible to oxidative damage showing only increase in protein carbonyl groups and depleted GSH. Taken together, the prefrontal cortex seems to be more sensitive to oxidative stress than the hippocampus following chronic isolation stress, which may be relevant for further research related to stress-induced depressive-like behavior.",
journal = "Molecular and Cellular Biochemistry",
title = "Different susceptibility of prefrontal cortex and hippocampus to oxidative stress following chronic social isolation stress",
volume = "393",
number = "1-2",
pages = "43-57",
doi = "10.1007/s11010-014-2045-z"
}
Martinović, J., Todorović, N., Bošković, M., Pajović, S. B., Demajo, M.,& Filipović, D.. (2014). Different susceptibility of prefrontal cortex and hippocampus to oxidative stress following chronic social isolation stress. in Molecular and Cellular Biochemistry, 393(1-2), 43-57.
https://doi.org/10.1007/s11010-014-2045-z
Martinović J, Todorović N, Bošković M, Pajović SB, Demajo M, Filipović D. Different susceptibility of prefrontal cortex and hippocampus to oxidative stress following chronic social isolation stress. in Molecular and Cellular Biochemistry. 2014;393(1-2):43-57.
doi:10.1007/s11010-014-2045-z .
Martinović, Jelena, Todorović, Nevena, Bošković, Maja, Pajović, Snežana B., Demajo, Miroslav, Filipović, Dragana, "Different susceptibility of prefrontal cortex and hippocampus to oxidative stress following chronic social isolation stress" in Molecular and Cellular Biochemistry, 393, no. 1-2 (2014):43-57,
https://doi.org/10.1007/s11010-014-2045-z . .
77
73
78

Effects of nitric oxide production on glutathione levels in an animal model of depression

Filipović, Dragana; Zlatković, Jelena; Demajo, Miroslav

(Society of Physical Chemists of Serbia, 2012)

TY  - CONF
AU  - Filipović, Dragana
AU  - Zlatković, Jelena
AU  - Demajo, Miroslav
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9279
AB  - Stimulation of glutamate receptors induces neuronal nitric oxide (NO) release,
which in turn modulates glutamate transmission. The present study evaluated the
effects of acute, chronic or combined stress on NO production via the accumulation
of nitrite, the stable metabolite of NO, in the prefrontal cortex and hippocampus of
male Wistar rats. Given that glutathione (GSH) plays a critical role in protecting
cells from oxidative stress, as well as maintaining the thiol redox state, GSH levels
in cytosolic fractions of both brain structures were examined. A significant increase
in nitrite levels was obtained after 3 weeks of chronic isolation stress, followed by
combined stresses (chronic + acute stress). Moreover, GSH levels were
significantly decreased after chronic and both combined stresses in both brain
structures. Our data support the idea that GSH might represent an important buffer
of NO toxicity in the brain, indicating that compromised redox buffering controlled
by GSH makes neuronal cells susceptible to endogenous physiological flux of NO.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
T1  - Effects of nitric oxide production on glutathione levels in an animal model of depression
VL  - 1
SP  - 352
EP  - 354
ER  - 
@conference{
author = "Filipović, Dragana and Zlatković, Jelena and Demajo, Miroslav",
year = "2012",
abstract = "Stimulation of glutamate receptors induces neuronal nitric oxide (NO) release,
which in turn modulates glutamate transmission. The present study evaluated the
effects of acute, chronic or combined stress on NO production via the accumulation
of nitrite, the stable metabolite of NO, in the prefrontal cortex and hippocampus of
male Wistar rats. Given that glutathione (GSH) plays a critical role in protecting
cells from oxidative stress, as well as maintaining the thiol redox state, GSH levels
in cytosolic fractions of both brain structures were examined. A significant increase
in nitrite levels was obtained after 3 weeks of chronic isolation stress, followed by
combined stresses (chronic + acute stress). Moreover, GSH levels were
significantly decreased after chronic and both combined stresses in both brain
structures. Our data support the idea that GSH might represent an important buffer
of NO toxicity in the brain, indicating that compromised redox buffering controlled
by GSH makes neuronal cells susceptible to endogenous physiological flux of NO.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry",
title = "Effects of nitric oxide production on glutathione levels in an animal model of depression",
volume = "1",
pages = "352-354"
}
Filipović, D., Zlatković, J.,& Demajo, M.. (2012). Effects of nitric oxide production on glutathione levels in an animal model of depression. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 352-354.
Filipović D, Zlatković J, Demajo M. Effects of nitric oxide production on glutathione levels in an animal model of depression. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry. 2012;1:352-354..
Filipović, Dragana, Zlatković, Jelena, Demajo, Miroslav, "Effects of nitric oxide production on glutathione levels in an animal model of depression" in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry, 1 (2012):352-354.

Nitric oxide and protein carbonyl content in the liver of stressed rats

Zlatković, Jelena; Demajo, Miroslav; Filipović, Dragana

(Society of Physical Chemists of Serbia, 2012)

TY  - CONF
AU  - Zlatković, Jelena
AU  - Demajo, Miroslav
AU  - Filipović, Dragana
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9282
AB  - Nitric oxide (NO) has been identified as a source of oxidative/nitrosative stress that
is known to oxidatively modify DNA, lipids and proteins. One such modification is
the addition of carbonyl groups to amino acid residues in proteins. Therefore, the
aim of our study was to examine the effects of acute, chronic or combined stress on
NO production and protein carbonyl content in the cytosolic fraction of rat liver.
Since NO is a highly reactive molecule, the levels of NO metabolites (nitrates and
nitrites) as markers of stable end products of NO metabolism were measured. Both
acute stresses showed unchanged nitrite levels while only acute IM stress led to an
increased level of the carbonyl group. The NO metabolites and protein carbonyl
content were increased by chronic isolation and remained upregulated after
combined stress. These data indicate that chronic isolation stress with increased
NO metabolites led to nitrosative stress, whereby accumulation of oxidized
proteins in the liver may induce progressive liver damage.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
T1  - Nitric oxide and protein carbonyl content in the liver of stressed rats
VL  - 1
SP  - 358
EP  - 360
ER  - 
@conference{
author = "Zlatković, Jelena and Demajo, Miroslav and Filipović, Dragana",
year = "2012",
abstract = "Nitric oxide (NO) has been identified as a source of oxidative/nitrosative stress that
is known to oxidatively modify DNA, lipids and proteins. One such modification is
the addition of carbonyl groups to amino acid residues in proteins. Therefore, the
aim of our study was to examine the effects of acute, chronic or combined stress on
NO production and protein carbonyl content in the cytosolic fraction of rat liver.
Since NO is a highly reactive molecule, the levels of NO metabolites (nitrates and
nitrites) as markers of stable end products of NO metabolism were measured. Both
acute stresses showed unchanged nitrite levels while only acute IM stress led to an
increased level of the carbonyl group. The NO metabolites and protein carbonyl
content were increased by chronic isolation and remained upregulated after
combined stress. These data indicate that chronic isolation stress with increased
NO metabolites led to nitrosative stress, whereby accumulation of oxidized
proteins in the liver may induce progressive liver damage.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry",
title = "Nitric oxide and protein carbonyl content in the liver of stressed rats",
volume = "1",
pages = "358-360"
}
Zlatković, J., Demajo, M.,& Filipović, D.. (2012). Nitric oxide and protein carbonyl content in the liver of stressed rats. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 358-360.
Zlatković J, Demajo M, Filipović D. Nitric oxide and protein carbonyl content in the liver of stressed rats. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry. 2012;1:358-360..
Zlatković, Jelena, Demajo, Miroslav, Filipović, Dragana, "Nitric oxide and protein carbonyl content in the liver of stressed rats" in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry, 1 (2012):358-360.

Chronic isolation stress compromises JNK/c-Jun signaling in rat brain

Filipović, Dragana; Martinović, Jelena; Pavicevic, Ivan; Mandic, Ljuba; Demajo, Miroslav

(2012)

TY  - JOUR
AU  - Filipović, Dragana
AU  - Martinović, Jelena
AU  - Pavicevic, Ivan
AU  - Mandic, Ljuba
AU  - Demajo, Miroslav
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4360
AB  - The c-Jun NH2-terminal kinases (JNKs) are important stress-responsive kinases. They regulate cellular activities by sequential phosphorylation and activation through a mitogen-activated protein kinase cascade, whereas JNKs activation is altered in response to various stressors. In the present study, we used immunoblotting to assess the effect of 21 day of social isolation as the chronic stressor, either sole and in combination with 2 h of acute immobilization or cold (4A degrees C) stress on circulating corticosterone level and phosphorylation status of p46 (phospho-p46/total p46) and p54 (phospho-p54/total p54) JNK isoforms in the cytosolic and nuclear fraction of the prefrontal cortex and hippocampus of male Wistar rats. Also, the phosphorylation status of JNK nuclear down-stream target c-Jun (p-c-Jun/c-Jun) on Ser63 was examined. Both acute stressors with elevated CORT levels led to increased phosphorylation status of cytosolic p54 JNK isoforms but not p46 JNK isoforms only in the hippocampus and no change in phosphorylation status of c-jun in both brain regions. Chronic isolation with unaltered CORT level and reduced responsiveness to novel acute stressors, led to unchanged or reduced phosphorylation status of p46 and p54 JNK isoforms in both fractions and both brain regions, whereas the decrease of c-Jun phosphorylation status was found only in the prefrontal cortex. Our results suggest that compromised JNKs activation following chronic isolation may lead to interruption of JNK signaling, which could be related with neuropsychiatric disorders such as depression or long-lasting neuronal remodeling.
T2  - Journal of Neural Transmission
T1  - Chronic isolation stress compromises JNK/c-Jun signaling in rat brain
VL  - 119
IS  - 11
SP  - 1275
EP  - 1284
DO  - 10.1007/s00702-012-0776-0
ER  - 
@article{
author = "Filipović, Dragana and Martinović, Jelena and Pavicevic, Ivan and Mandic, Ljuba and Demajo, Miroslav",
year = "2012",
abstract = "The c-Jun NH2-terminal kinases (JNKs) are important stress-responsive kinases. They regulate cellular activities by sequential phosphorylation and activation through a mitogen-activated protein kinase cascade, whereas JNKs activation is altered in response to various stressors. In the present study, we used immunoblotting to assess the effect of 21 day of social isolation as the chronic stressor, either sole and in combination with 2 h of acute immobilization or cold (4A degrees C) stress on circulating corticosterone level and phosphorylation status of p46 (phospho-p46/total p46) and p54 (phospho-p54/total p54) JNK isoforms in the cytosolic and nuclear fraction of the prefrontal cortex and hippocampus of male Wistar rats. Also, the phosphorylation status of JNK nuclear down-stream target c-Jun (p-c-Jun/c-Jun) on Ser63 was examined. Both acute stressors with elevated CORT levels led to increased phosphorylation status of cytosolic p54 JNK isoforms but not p46 JNK isoforms only in the hippocampus and no change in phosphorylation status of c-jun in both brain regions. Chronic isolation with unaltered CORT level and reduced responsiveness to novel acute stressors, led to unchanged or reduced phosphorylation status of p46 and p54 JNK isoforms in both fractions and both brain regions, whereas the decrease of c-Jun phosphorylation status was found only in the prefrontal cortex. Our results suggest that compromised JNKs activation following chronic isolation may lead to interruption of JNK signaling, which could be related with neuropsychiatric disorders such as depression or long-lasting neuronal remodeling.",
journal = "Journal of Neural Transmission",
title = "Chronic isolation stress compromises JNK/c-Jun signaling in rat brain",
volume = "119",
number = "11",
pages = "1275-1284",
doi = "10.1007/s00702-012-0776-0"
}
Filipović, D., Martinović, J., Pavicevic, I., Mandic, L.,& Demajo, M.. (2012). Chronic isolation stress compromises JNK/c-Jun signaling in rat brain. in Journal of Neural Transmission, 119(11), 1275-1284.
https://doi.org/10.1007/s00702-012-0776-0
Filipović D, Martinović J, Pavicevic I, Mandic L, Demajo M. Chronic isolation stress compromises JNK/c-Jun signaling in rat brain. in Journal of Neural Transmission. 2012;119(11):1275-1284.
doi:10.1007/s00702-012-0776-0 .
Filipović, Dragana, Martinović, Jelena, Pavicevic, Ivan, Mandic, Ljuba, Demajo, Miroslav, "Chronic isolation stress compromises JNK/c-Jun signaling in rat brain" in Journal of Neural Transmission, 119, no. 11 (2012):1275-1284,
https://doi.org/10.1007/s00702-012-0776-0 . .
5
2
4

Detection of elements and radioactivity in pellets from long-eared owls (Asio otus) inhabiting the city of Belgrade (Serbia)

Demajo, Miroslav; Cvetićanin, Jelena M.; Stoiljković, Milovan; Trpkov, Đorđe; Andrić, Velibor; Onjia, Antonije E.; Nešković, Olivera M.

(2011)

TY  - JOUR
AU  - Demajo, Miroslav
AU  - Cvetićanin, Jelena M.
AU  - Stoiljković, Milovan
AU  - Trpkov, Đorđe
AU  - Andrić, Velibor
AU  - Onjia, Antonije E.
AU  - Nešković, Olivera M.
PY  - 2011
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4664
AB  - In this study, we analysed pellets from long-eared owls (Asio otus) collected from four localities in Belgrade (Serbia). The pellets contained the remains of prey, namely voles (Arvicola terrestris) and field mice (Apodemus agrarius). The concentrations of 14 elements (Ca, P, Mg, Na, K, Fe, Zn, Sr, Ba, Mn, Ti, Cu, Si, B) were evaluated in whole pellets and in samples containing only bone tissue, which were dissected from the whole pellet. The increased levels of certain elements, including Mn, Zn, Ba, Cu and radioactive K-40, indicate contamination of the soil by various sources, such as industrial plants and agricultural practices. From the results presented in this article, we suggest that the analysis of owl pellets may indicate the quality of the local environment.
T2  - Chemistry and Ecology
T1  - Detection of elements and radioactivity in pellets from long-eared owls (Asio otus) inhabiting the city of Belgrade (Serbia)
VL  - 27
IS  - 5
SP  - 393
EP  - 400
DO  - 10.1080/02757540.2011.607440
ER  - 
@article{
author = "Demajo, Miroslav and Cvetićanin, Jelena M. and Stoiljković, Milovan and Trpkov, Đorđe and Andrić, Velibor and Onjia, Antonije E. and Nešković, Olivera M.",
year = "2011",
abstract = "In this study, we analysed pellets from long-eared owls (Asio otus) collected from four localities in Belgrade (Serbia). The pellets contained the remains of prey, namely voles (Arvicola terrestris) and field mice (Apodemus agrarius). The concentrations of 14 elements (Ca, P, Mg, Na, K, Fe, Zn, Sr, Ba, Mn, Ti, Cu, Si, B) were evaluated in whole pellets and in samples containing only bone tissue, which were dissected from the whole pellet. The increased levels of certain elements, including Mn, Zn, Ba, Cu and radioactive K-40, indicate contamination of the soil by various sources, such as industrial plants and agricultural practices. From the results presented in this article, we suggest that the analysis of owl pellets may indicate the quality of the local environment.",
journal = "Chemistry and Ecology",
title = "Detection of elements and radioactivity in pellets from long-eared owls (Asio otus) inhabiting the city of Belgrade (Serbia)",
volume = "27",
number = "5",
pages = "393-400",
doi = "10.1080/02757540.2011.607440"
}
Demajo, M., Cvetićanin, J. M., Stoiljković, M., Trpkov, Đ., Andrić, V., Onjia, A. E.,& Nešković, O. M.. (2011). Detection of elements and radioactivity in pellets from long-eared owls (Asio otus) inhabiting the city of Belgrade (Serbia). in Chemistry and Ecology, 27(5), 393-400.
https://doi.org/10.1080/02757540.2011.607440
Demajo M, Cvetićanin JM, Stoiljković M, Trpkov Đ, Andrić V, Onjia AE, Nešković OM. Detection of elements and radioactivity in pellets from long-eared owls (Asio otus) inhabiting the city of Belgrade (Serbia). in Chemistry and Ecology. 2011;27(5):393-400.
doi:10.1080/02757540.2011.607440 .
Demajo, Miroslav, Cvetićanin, Jelena M., Stoiljković, Milovan, Trpkov, Đorđe, Andrić, Velibor, Onjia, Antonije E., Nešković, Olivera M., "Detection of elements and radioactivity in pellets from long-eared owls (Asio otus) inhabiting the city of Belgrade (Serbia)" in Chemistry and Ecology, 27, no. 5 (2011):393-400,
https://doi.org/10.1080/02757540.2011.607440 . .
1
1
1

Pellets from long-eared owls (asio otus) as indicators of soil quality

Demajo, Miroslav; Cvetićanin, Jelena M.; Stoiljković, Milovan; Trpkov, Đorđe; Andrić, Velibor; Onjia, Antonije E.; Nešković, Olivera M.

(Society of Physical Chemists of Serbia, 2010)

TY  - CONF
AU  - Demajo, Miroslav
AU  - Cvetićanin, Jelena M.
AU  - Stoiljković, Milovan
AU  - Trpkov, Đorđe
AU  - Andrić, Velibor
AU  - Onjia, Antonije E.
AU  - Nešković, Olivera M.
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9338
AB  - Pellets from Long-Eared Owls (Asio otus) were collected from four localities in the
city and in the vicinity of Belgrade. The element content in the pellets was
analyzed. The results indicate that owl pellets may be useful for monitoring
environmental conditions, specifically soil quality.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - Pellets from long-eared owls (asio otus) as indicators of soil quality
ER  - 
@conference{
author = "Demajo, Miroslav and Cvetićanin, Jelena M. and Stoiljković, Milovan and Trpkov, Đorđe and Andrić, Velibor and Onjia, Antonije E. and Nešković, Olivera M.",
year = "2010",
abstract = "Pellets from Long-Eared Owls (Asio otus) were collected from four localities in the
city and in the vicinity of Belgrade. The element content in the pellets was
analyzed. The results indicate that owl pellets may be useful for monitoring
environmental conditions, specifically soil quality.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "Pellets from long-eared owls (asio otus) as indicators of soil quality"
}
Demajo, M., Cvetićanin, J. M., Stoiljković, M., Trpkov, Đ., Andrić, V., Onjia, A. E.,& Nešković, O. M.. (2010). Pellets from long-eared owls (asio otus) as indicators of soil quality. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
Demajo M, Cvetićanin JM, Stoiljković M, Trpkov Đ, Andrić V, Onjia AE, Nešković OM. Pellets from long-eared owls (asio otus) as indicators of soil quality. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;..
Demajo, Miroslav, Cvetićanin, Jelena M., Stoiljković, Milovan, Trpkov, Đorđe, Andrić, Velibor, Onjia, Antonije E., Nešković, Olivera M., "Pellets from long-eared owls (asio otus) as indicators of soil quality" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010).

Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus

Horvat, Anica; Milošević, Maja; Demajo, Miroslav

(2009)

TY  - JOUR
AU  - Horvat, Anica
AU  - Milošević, Maja
AU  - Demajo, Miroslav
PY  - 2009
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3630
AB  - In this study, we investigated the in vitro effects of metal ions on Mg2+-ATPase activity in isolated membranes from rat uterus. The effects of increasing concentrations of metal salts (CrCl2, CuSO4, HgCl2 and ZnSO4) show sigmoidal and almost complete inhibition relative to the control enzyme activity. According to the IC50, the ATPase possesses greater sensibility to Zn2+ GT Cu2+ congruent to Cr3+ congruent to Hg2+, while other metal salts exhibit the following inhibition: CdCl2 55%, CsCl 64.5% and SrCl2 58%. Here we demonstrated that the physicochemical properties of these metals are of importance in defining possible mechanisms of binding and decrease of enzyme activity.
T2  - Environmental Chemistry Letters
T1  - Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus
VL  - 7
IS  - 1
SP  - 41
EP  - 44
DO  - 10.1007/s10311-007-0132-z
ER  - 
@article{
author = "Horvat, Anica and Milošević, Maja and Demajo, Miroslav",
year = "2009",
abstract = "In this study, we investigated the in vitro effects of metal ions on Mg2+-ATPase activity in isolated membranes from rat uterus. The effects of increasing concentrations of metal salts (CrCl2, CuSO4, HgCl2 and ZnSO4) show sigmoidal and almost complete inhibition relative to the control enzyme activity. According to the IC50, the ATPase possesses greater sensibility to Zn2+ GT Cu2+ congruent to Cr3+ congruent to Hg2+, while other metal salts exhibit the following inhibition: CdCl2 55%, CsCl 64.5% and SrCl2 58%. Here we demonstrated that the physicochemical properties of these metals are of importance in defining possible mechanisms of binding and decrease of enzyme activity.",
journal = "Environmental Chemistry Letters",
title = "Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus",
volume = "7",
number = "1",
pages = "41-44",
doi = "10.1007/s10311-007-0132-z"
}
Horvat, A., Milošević, M.,& Demajo, M.. (2009). Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus. in Environmental Chemistry Letters, 7(1), 41-44.
https://doi.org/10.1007/s10311-007-0132-z
Horvat A, Milošević M, Demajo M. Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus. in Environmental Chemistry Letters. 2009;7(1):41-44.
doi:10.1007/s10311-007-0132-z .
Horvat, Anica, Milošević, Maja, Demajo, Miroslav, "Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus" in Environmental Chemistry Letters, 7, no. 1 (2009):41-44,
https://doi.org/10.1007/s10311-007-0132-z . .
1
1
1

Quantification of Transforming Growth Factor Beta 1 Levels in Metastatic Axillary Lymph Node Tissue Extracts from Breast Cancer Patients A New Specimen Source

Ivanović, Vesna; Dedović-Tanić, Nasta; Milovanović, Zorka M.; Lukić, Silvana; Nikolic, Srdjan; Baltic, Vladimir; Stojiljković, Bratislav; Budisin, Nikola; Savovski, Kiril; Demajo, Miroslav; Dimitrijević, Bogomir B.

(2009)

TY  - JOUR
AU  - Ivanović, Vesna
AU  - Dedović-Tanić, Nasta
AU  - Milovanović, Zorka M.
AU  - Lukić, Silvana
AU  - Nikolic, Srdjan
AU  - Baltic, Vladimir
AU  - Stojiljković, Bratislav
AU  - Budisin, Nikola
AU  - Savovski, Kiril
AU  - Demajo, Miroslav
AU  - Dimitrijević, Bogomir B.
PY  - 2009
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/2728
AB  - OBJECTIVE: To use cytoplasmic tissue extract as a new specimen source to quantify transforming growth factor beta 1 (TGF beta 1) protein in metastatic axillary lymph node tissue (ALNT) of breast cancer (BC) patients and to confirm the feasibility of this approach in a prospective pilot study on a subgroup of patients with invasive BC. STUDY DESIGN: The 6 selected malignant and autologous nonmalignant pairs of ALNT were fractionated, under special preanalytical, nonaggressive/nondenaturing conditions, to obtain respective cytoplasmic extracts for TGF beta 1 detection by the Quantikine (R and D Systems Inc., Minneapolis, Minnesota, U.S.A.) enzyme-linked immunosorbent assay kit. RESULTS: The data indicated a highly significant (r=0.973054) positive linear correlation between the TGF beta 1 concentration and total protein concentration in cytoplasmic extract of metastatic ALNT. The subsequent patients pilot study, performed strictly before any clinicopathologic factors were accessible, revealed significantly (p LT 0.01) elevated TGF beta 1 in malignant ALNT (median value: 1.05 ng/mg protein, range: 0.67-3.6 ng/mg protein, n=6) vs. autologous nonmalignant ALNT controls (median value: 0.48 ng/mg protein, range: 0.29-0.90 ng/mg protein, n=6). This elevation was correlated with the number of metastatic axillary lymph nodes with respect to the total and was consistent with an increase in size of tumor deposits in axillary lymph nodes. CONCLUSION: Our data provide for the first time suggestive evidence that the TGF beta 1 level in cytoplasmic extracts of metastatic ALNTs may be a promising bio-marker of invasiveness for BC patients. Confirmatory, large-scale studies are needed to evaluate possible implications of this putative biomarker in BC diagnosis and treatment. (Anal Quant Cytol Histol 2009;31:288-295)
T2  - Analytical and Quantitative Cytology and Histology
T1  - Quantification of Transforming Growth Factor Beta 1 Levels in Metastatic Axillary Lymph Node Tissue Extracts from Breast Cancer Patients A New Specimen Source
VL  - 31
IS  - 5
SP  - 288
EP  - 295
ER  - 
@article{
author = "Ivanović, Vesna and Dedović-Tanić, Nasta and Milovanović, Zorka M. and Lukić, Silvana and Nikolic, Srdjan and Baltic, Vladimir and Stojiljković, Bratislav and Budisin, Nikola and Savovski, Kiril and Demajo, Miroslav and Dimitrijević, Bogomir B.",
year = "2009",
abstract = "OBJECTIVE: To use cytoplasmic tissue extract as a new specimen source to quantify transforming growth factor beta 1 (TGF beta 1) protein in metastatic axillary lymph node tissue (ALNT) of breast cancer (BC) patients and to confirm the feasibility of this approach in a prospective pilot study on a subgroup of patients with invasive BC. STUDY DESIGN: The 6 selected malignant and autologous nonmalignant pairs of ALNT were fractionated, under special preanalytical, nonaggressive/nondenaturing conditions, to obtain respective cytoplasmic extracts for TGF beta 1 detection by the Quantikine (R and D Systems Inc., Minneapolis, Minnesota, U.S.A.) enzyme-linked immunosorbent assay kit. RESULTS: The data indicated a highly significant (r=0.973054) positive linear correlation between the TGF beta 1 concentration and total protein concentration in cytoplasmic extract of metastatic ALNT. The subsequent patients pilot study, performed strictly before any clinicopathologic factors were accessible, revealed significantly (p LT 0.01) elevated TGF beta 1 in malignant ALNT (median value: 1.05 ng/mg protein, range: 0.67-3.6 ng/mg protein, n=6) vs. autologous nonmalignant ALNT controls (median value: 0.48 ng/mg protein, range: 0.29-0.90 ng/mg protein, n=6). This elevation was correlated with the number of metastatic axillary lymph nodes with respect to the total and was consistent with an increase in size of tumor deposits in axillary lymph nodes. CONCLUSION: Our data provide for the first time suggestive evidence that the TGF beta 1 level in cytoplasmic extracts of metastatic ALNTs may be a promising bio-marker of invasiveness for BC patients. Confirmatory, large-scale studies are needed to evaluate possible implications of this putative biomarker in BC diagnosis and treatment. (Anal Quant Cytol Histol 2009;31:288-295)",
journal = "Analytical and Quantitative Cytology and Histology",
title = "Quantification of Transforming Growth Factor Beta 1 Levels in Metastatic Axillary Lymph Node Tissue Extracts from Breast Cancer Patients A New Specimen Source",
volume = "31",
number = "5",
pages = "288-295"
}
Ivanović, V., Dedović-Tanić, N., Milovanović, Z. M., Lukić, S., Nikolic, S., Baltic, V., Stojiljković, B., Budisin, N., Savovski, K., Demajo, M.,& Dimitrijević, B. B.. (2009). Quantification of Transforming Growth Factor Beta 1 Levels in Metastatic Axillary Lymph Node Tissue Extracts from Breast Cancer Patients A New Specimen Source. in Analytical and Quantitative Cytology and Histology, 31(5), 288-295.
Ivanović V, Dedović-Tanić N, Milovanović ZM, Lukić S, Nikolic S, Baltic V, Stojiljković B, Budisin N, Savovski K, Demajo M, Dimitrijević BB. Quantification of Transforming Growth Factor Beta 1 Levels in Metastatic Axillary Lymph Node Tissue Extracts from Breast Cancer Patients A New Specimen Source. in Analytical and Quantitative Cytology and Histology. 2009;31(5):288-295..
Ivanović, Vesna, Dedović-Tanić, Nasta, Milovanović, Zorka M., Lukić, Silvana, Nikolic, Srdjan, Baltic, Vladimir, Stojiljković, Bratislav, Budisin, Nikola, Savovski, Kiril, Demajo, Miroslav, Dimitrijević, Bogomir B., "Quantification of Transforming Growth Factor Beta 1 Levels in Metastatic Axillary Lymph Node Tissue Extracts from Breast Cancer Patients A New Specimen Source" in Analytical and Quantitative Cytology and Histology, 31, no. 5 (2009):288-295.
4

Liver glucocorticoid receptor and heat shock protein 70 levels in rats exposed to different stress models

Filipović, Dragana; Gavrilović, Ljubica; Dronjak, Slađana; Demajo, Miroslav; Radoičić, Marija B.

(2008)

TY  - JOUR
AU  - Filipović, Dragana
AU  - Gavrilović, Ljubica
AU  - Dronjak, Slađana
AU  - Demajo, Miroslav
AU  - Radoičić, Marija B.
PY  - 2008
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3477
AB  - The aim of the present study was to define the stress-induced pattern of cytosolic glucocorticoid receptor (GR) and Hsp70 protein in the liver of male Wistar rats exposed to different stress models: acute (2 h/day) immobilization or cold (4 degrees C); chronic (21 days) isolation, crowding, swimming or isolation plus swimming and combined (chronic plus acute stress). Changes in plasma levels of corticosterone were studied by radioimmunoassay (RIA). The results obtained by Western immunoblotting showed that both acute stressors led to a significant decrease in cytosolic GR and Hsp70 levels. Compared to acute stress effects, only a weak decrease in the levels of GR and Hsp70 was demonstrated in chronic stress models. Chronically stressed rats, which were subsequently exposed to novel acute stressors (immobilization or cold), showed a lower extent of GR down-regulation when compared to acute stress. The exception was swimming, which partially restores this down-regulation. The observed changes in the levels of these major stress-related cellular proteins in liver cytosol lead to the conclusion that chronic stressors compromise intracellular GR down-regulation in the liver.
T2  - Physiological Research
T1  - Liver glucocorticoid receptor and heat shock protein 70 levels in rats exposed to different stress models
VL  - 57
IS  - 2
SP  - 205
EP  - 213
ER  - 
@article{
author = "Filipović, Dragana and Gavrilović, Ljubica and Dronjak, Slađana and Demajo, Miroslav and Radoičić, Marija B.",
year = "2008",
abstract = "The aim of the present study was to define the stress-induced pattern of cytosolic glucocorticoid receptor (GR) and Hsp70 protein in the liver of male Wistar rats exposed to different stress models: acute (2 h/day) immobilization or cold (4 degrees C); chronic (21 days) isolation, crowding, swimming or isolation plus swimming and combined (chronic plus acute stress). Changes in plasma levels of corticosterone were studied by radioimmunoassay (RIA). The results obtained by Western immunoblotting showed that both acute stressors led to a significant decrease in cytosolic GR and Hsp70 levels. Compared to acute stress effects, only a weak decrease in the levels of GR and Hsp70 was demonstrated in chronic stress models. Chronically stressed rats, which were subsequently exposed to novel acute stressors (immobilization or cold), showed a lower extent of GR down-regulation when compared to acute stress. The exception was swimming, which partially restores this down-regulation. The observed changes in the levels of these major stress-related cellular proteins in liver cytosol lead to the conclusion that chronic stressors compromise intracellular GR down-regulation in the liver.",
journal = "Physiological Research",
title = "Liver glucocorticoid receptor and heat shock protein 70 levels in rats exposed to different stress models",
volume = "57",
number = "2",
pages = "205-213"
}
Filipović, D., Gavrilović, L., Dronjak, S., Demajo, M.,& Radoičić, M. B.. (2008). Liver glucocorticoid receptor and heat shock protein 70 levels in rats exposed to different stress models. in Physiological Research, 57(2), 205-213.
Filipović D, Gavrilović L, Dronjak S, Demajo M, Radoičić MB. Liver glucocorticoid receptor and heat shock protein 70 levels in rats exposed to different stress models. in Physiological Research. 2008;57(2):205-213..
Filipović, Dragana, Gavrilović, Ljubica, Dronjak, Slađana, Demajo, Miroslav, Radoičić, Marija B., "Liver glucocorticoid receptor and heat shock protein 70 levels in rats exposed to different stress models" in Physiological Research, 57, no. 2 (2008):205-213.
11

Traces of DU in samples of environmental bio-monitors (non-flowering plants, fungi) and soil from target sites of the Western Balkan region

Žunić, Zora S.; Mietelski, Jerzy W.; Blazej, Sylwia; Gaca, Pawel; Tomankiewicz, Ewa; Ujić, Predrag; Čeliković, Igor T.; Cuknic, Olivera; Demajo, Miroslav

(2008)

TY  - JOUR
AU  - Žunić, Zora S.
AU  - Mietelski, Jerzy W.
AU  - Blazej, Sylwia
AU  - Gaca, Pawel
AU  - Tomankiewicz, Ewa
AU  - Ujić, Predrag
AU  - Čeliković, Igor T.
AU  - Cuknic, Olivera
AU  - Demajo, Miroslav
PY  - 2008
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3496
AB  - This paper reports results of gamma and alpha spectrometric measurements for mosses, lichens, fungi and soil samples from areas in the Balkans targeted by depleted uranium (DU). Samples were collected in 2002 and 2003 in the vicinity of several villages, principally Han Pijesak: (Bosnia and Herzegovina, hit by DU in 1995) and Bratoselce (South Serbia, hit by DU in 1999) and in lesser numbers from Gornja Stubla, Kosovo (which is identified as a high natural radon/thoron area) and Presevo close to the Kosovo border. In the course of gamma spectrometric measurements some results suggested samples with unusual high uranium contents which might be considered to be a signature for the presence of DU, although many samples had very high detection limits. Alpha spectrometric measurements directly proved the presence of DU for five samples, all from directly targeted places. These were samples of mosses, lichens and soil. For some samples homogeneity tests were applied which showed a rather even distribution of DU in these samples. No trace of DU was found in any sample from a dwelling. (C) 2008 Elsevier Ltd. All rights reserved.
T2  - Journal of Environmental Radioactivity
T1  - Traces of DU in samples of environmental bio-monitors (non-flowering plants, fungi) and soil from target sites of the Western Balkan region
VL  - 99
IS  - 8
SP  - 1324
EP  - 1328
DO  - 10.1016/j.jenvrad.2008.04.005
ER  - 
@article{
author = "Žunić, Zora S. and Mietelski, Jerzy W. and Blazej, Sylwia and Gaca, Pawel and Tomankiewicz, Ewa and Ujić, Predrag and Čeliković, Igor T. and Cuknic, Olivera and Demajo, Miroslav",
year = "2008",
abstract = "This paper reports results of gamma and alpha spectrometric measurements for mosses, lichens, fungi and soil samples from areas in the Balkans targeted by depleted uranium (DU). Samples were collected in 2002 and 2003 in the vicinity of several villages, principally Han Pijesak: (Bosnia and Herzegovina, hit by DU in 1995) and Bratoselce (South Serbia, hit by DU in 1999) and in lesser numbers from Gornja Stubla, Kosovo (which is identified as a high natural radon/thoron area) and Presevo close to the Kosovo border. In the course of gamma spectrometric measurements some results suggested samples with unusual high uranium contents which might be considered to be a signature for the presence of DU, although many samples had very high detection limits. Alpha spectrometric measurements directly proved the presence of DU for five samples, all from directly targeted places. These were samples of mosses, lichens and soil. For some samples homogeneity tests were applied which showed a rather even distribution of DU in these samples. No trace of DU was found in any sample from a dwelling. (C) 2008 Elsevier Ltd. All rights reserved.",
journal = "Journal of Environmental Radioactivity",
title = "Traces of DU in samples of environmental bio-monitors (non-flowering plants, fungi) and soil from target sites of the Western Balkan region",
volume = "99",
number = "8",
pages = "1324-1328",
doi = "10.1016/j.jenvrad.2008.04.005"
}
Žunić, Z. S., Mietelski, J. W., Blazej, S., Gaca, P., Tomankiewicz, E., Ujić, P., Čeliković, I. T., Cuknic, O.,& Demajo, M.. (2008). Traces of DU in samples of environmental bio-monitors (non-flowering plants, fungi) and soil from target sites of the Western Balkan region. in Journal of Environmental Radioactivity, 99(8), 1324-1328.
https://doi.org/10.1016/j.jenvrad.2008.04.005
Žunić ZS, Mietelski JW, Blazej S, Gaca P, Tomankiewicz E, Ujić P, Čeliković IT, Cuknic O, Demajo M. Traces of DU in samples of environmental bio-monitors (non-flowering plants, fungi) and soil from target sites of the Western Balkan region. in Journal of Environmental Radioactivity. 2008;99(8):1324-1328.
doi:10.1016/j.jenvrad.2008.04.005 .
Žunić, Zora S., Mietelski, Jerzy W., Blazej, Sylwia, Gaca, Pawel, Tomankiewicz, Ewa, Ujić, Predrag, Čeliković, Igor T., Cuknic, Olivera, Demajo, Miroslav, "Traces of DU in samples of environmental bio-monitors (non-flowering plants, fungi) and soil from target sites of the Western Balkan region" in Journal of Environmental Radioactivity, 99, no. 8 (2008):1324-1328,
https://doi.org/10.1016/j.jenvrad.2008.04.005 . .
10
10
14

Different induction of dual corticosteroid receptor system in the rat hippocampus following gamma radiation

Veličković, Nataša; Đorđević, Ana D.; Popović, Nataša; Demajo, Miroslav

(Society of Physical Chemists of Serbia, 2006)

TY  - CONF
AU  - Veličković, Nataša
AU  - Đorđević, Ana D.
AU  - Popović, Nataša
AU  - Demajo, Miroslav
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9434
AB  - Cranial radiotherapy (CRT) is an effective way to prevent CNS relapse in children with acute lymphoblastic leukemia (ALL). However, CRT also has serious side effects on normal tissues, including long-term neuroendocrine disturbances. In order to test this clinical protocol on animals, we examined the effects of CRT (10 Gy) on the level of mRNA for glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) in the hippocampus of 8-days-old rats. Irradiation rapidly stimulated GR gene expression in a time-dependent manner, whereas the time-course of MR mRNA expression showed no statistically significant changes. At postnatal day 42, the level of GR mRNA was diminished while the level of MR mRNA remained unchanged compared to matched controls. Dexamethasone suppression test (DST) revealed the altered nucleocytoplasmic shuttling of activated GR after CRT in 42-days-old rats, as a long-term consequence of gamma irradiation.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
T1  - Different induction of dual corticosteroid receptor system in the rat hippocampus following gamma radiation
SP  - 425
EP  - 427
ER  - 
@conference{
author = "Veličković, Nataša and Đorđević, Ana D. and Popović, Nataša and Demajo, Miroslav",
year = "2006",
abstract = "Cranial radiotherapy (CRT) is an effective way to prevent CNS relapse in children with acute lymphoblastic leukemia (ALL). However, CRT also has serious side effects on normal tissues, including long-term neuroendocrine disturbances. In order to test this clinical protocol on animals, we examined the effects of CRT (10 Gy) on the level of mRNA for glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) in the hippocampus of 8-days-old rats. Irradiation rapidly stimulated GR gene expression in a time-dependent manner, whereas the time-course of MR mRNA expression showed no statistically significant changes. At postnatal day 42, the level of GR mRNA was diminished while the level of MR mRNA remained unchanged compared to matched controls. Dexamethasone suppression test (DST) revealed the altered nucleocytoplasmic shuttling of activated GR after CRT in 42-days-old rats, as a long-term consequence of gamma irradiation.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry",
title = "Different induction of dual corticosteroid receptor system in the rat hippocampus following gamma radiation",
pages = "425-427"
}
Veličković, N., Đorđević, A. D., Popović, N.,& Demajo, M.. (2006). Different induction of dual corticosteroid receptor system in the rat hippocampus following gamma radiation. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 425-427.
Veličković N, Đorđević AD, Popović N, Demajo M. Different induction of dual corticosteroid receptor system in the rat hippocampus following gamma radiation. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry. 2006;:425-427..
Veličković, Nataša, Đorđević, Ana D., Popović, Nataša, Demajo, Miroslav, "Different induction of dual corticosteroid receptor system in the rat hippocampus following gamma radiation" in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry (2006):425-427.

Elevated plasma TGF-beta(1) levels correlate with decreased survival of metastatic breast cancer patients

Ivanović, Vesna; Demajo, Miroslav; Krtolica-Žikić, Koviljka; Krajnović, Milena M.; Dimitrijević, Bogomir B.; Konstantinovic, M.; Baltic, Vladimir; Prtenjak, Gordana; Stojiljković, Bratislav; Breberina, Milan; Nešković-Konstantinović, Zora; Nikolic-Vukosavljevic, Dragica

(2006)

TY  - JOUR
AU  - Ivanović, Vesna
AU  - Demajo, Miroslav
AU  - Krtolica-Žikić, Koviljka
AU  - Krajnović, Milena M.
AU  - Dimitrijević, Bogomir B.
AU  - Konstantinovic, M.
AU  - Baltic, Vladimir
AU  - Prtenjak, Gordana
AU  - Stojiljković, Bratislav
AU  - Breberina, Milan
AU  - Nešković-Konstantinović, Zora
AU  - Nikolic-Vukosavljevic, Dragica
PY  - 2006
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3079
AB  - Background: The role of circulating TGF-beta(1) in prognosis of breast cancer (BC) was investigated with an intention to define TGF-beta(1)-dependent high risk and low risk subsets of patients. Methods: Fifty three BC patients of all clinical stages and 37 healthy donors (HD) were analyzed for plasma TGF-beta(1) by the T beta RII receptor-based Quantikine TGF-beta(1) ELISA kit. Results: The plasma TGF-beta(1) level of Stage I/II disease (median: 0.94 ng/ml; n=10)) remained close to HD (median: 1.30 ng/ml; n = 37; p GT 0.1). In contrast, Stage III/IV disease (median: 2.34 ng/ml; n=43) exhibited highly significant TGF-beta(1) elevation (p LT 0.001) relative to HD. Further analysis revealed that TGF-beta(1) increase was predominantly attributed to Stage IV, metastatic disease patients (Q3=4.23 ng/ml) rather than to the group Stage III/IV (Q3=3.58 ng/ml). Using the plasma TGF-beta(1) concentration of 3.00 ng/ml as the cut-off value, two subgroups of patients were formed. Overall 2-year survival of the first subgroup, having elevated plasma TGF-beta(1) ( GT 3.00 ng/ml; n=10), was 10%. This was significantly decreased (p LT 0.05) compared to 52% survival observed for the second subgroup of patients with plasma TGF beta(1) values close to HD ( LT 3.00 ng/ml, n=19). Conclusion: We have performed a pilot study to determine the relationship between overall survival and TGF-beta(1) concentration in the blood of metastatic breast cancer patients. The survival was significantly reduced in the patients with elevated plasma TGF-beta(1) levels compared to that of the patients with plasma TGF-beta(1) levels close to normal. We propose that plasma TGF-beta(1) concentration may be a new tumour marker attributed to the presence of metastatic BC cells that may be used in selection of metastatic BC patients with poor prognosis. (c) 2006 Elsevier B.V. All rights reserved.
T2  - Clinica Chimica Acta
T1  - Elevated plasma TGF-beta(1) levels correlate with decreased survival of metastatic breast cancer patients
VL  - 371
IS  - 1-2
SP  - 191
EP  - 193
DO  - 10.1016/j.cca.2006.02.027
ER  - 
@article{
author = "Ivanović, Vesna and Demajo, Miroslav and Krtolica-Žikić, Koviljka and Krajnović, Milena M. and Dimitrijević, Bogomir B. and Konstantinovic, M. and Baltic, Vladimir and Prtenjak, Gordana and Stojiljković, Bratislav and Breberina, Milan and Nešković-Konstantinović, Zora and Nikolic-Vukosavljevic, Dragica",
year = "2006",
abstract = "Background: The role of circulating TGF-beta(1) in prognosis of breast cancer (BC) was investigated with an intention to define TGF-beta(1)-dependent high risk and low risk subsets of patients. Methods: Fifty three BC patients of all clinical stages and 37 healthy donors (HD) were analyzed for plasma TGF-beta(1) by the T beta RII receptor-based Quantikine TGF-beta(1) ELISA kit. Results: The plasma TGF-beta(1) level of Stage I/II disease (median: 0.94 ng/ml; n=10)) remained close to HD (median: 1.30 ng/ml; n = 37; p GT 0.1). In contrast, Stage III/IV disease (median: 2.34 ng/ml; n=43) exhibited highly significant TGF-beta(1) elevation (p LT 0.001) relative to HD. Further analysis revealed that TGF-beta(1) increase was predominantly attributed to Stage IV, metastatic disease patients (Q3=4.23 ng/ml) rather than to the group Stage III/IV (Q3=3.58 ng/ml). Using the plasma TGF-beta(1) concentration of 3.00 ng/ml as the cut-off value, two subgroups of patients were formed. Overall 2-year survival of the first subgroup, having elevated plasma TGF-beta(1) ( GT 3.00 ng/ml; n=10), was 10%. This was significantly decreased (p LT 0.05) compared to 52% survival observed for the second subgroup of patients with plasma TGF beta(1) values close to HD ( LT 3.00 ng/ml, n=19). Conclusion: We have performed a pilot study to determine the relationship between overall survival and TGF-beta(1) concentration in the blood of metastatic breast cancer patients. The survival was significantly reduced in the patients with elevated plasma TGF-beta(1) levels compared to that of the patients with plasma TGF-beta(1) levels close to normal. We propose that plasma TGF-beta(1) concentration may be a new tumour marker attributed to the presence of metastatic BC cells that may be used in selection of metastatic BC patients with poor prognosis. (c) 2006 Elsevier B.V. All rights reserved.",
journal = "Clinica Chimica Acta",
title = "Elevated plasma TGF-beta(1) levels correlate with decreased survival of metastatic breast cancer patients",
volume = "371",
number = "1-2",
pages = "191-193",
doi = "10.1016/j.cca.2006.02.027"
}
Ivanović, V., Demajo, M., Krtolica-Žikić, K., Krajnović, M. M., Dimitrijević, B. B., Konstantinovic, M., Baltic, V., Prtenjak, G., Stojiljković, B., Breberina, M., Nešković-Konstantinović, Z.,& Nikolic-Vukosavljevic, D.. (2006). Elevated plasma TGF-beta(1) levels correlate with decreased survival of metastatic breast cancer patients. in Clinica Chimica Acta, 371(1-2), 191-193.
https://doi.org/10.1016/j.cca.2006.02.027
Ivanović V, Demajo M, Krtolica-Žikić K, Krajnović MM, Dimitrijević BB, Konstantinovic M, Baltic V, Prtenjak G, Stojiljković B, Breberina M, Nešković-Konstantinović Z, Nikolic-Vukosavljevic D. Elevated plasma TGF-beta(1) levels correlate with decreased survival of metastatic breast cancer patients. in Clinica Chimica Acta. 2006;371(1-2):191-193.
doi:10.1016/j.cca.2006.02.027 .
Ivanović, Vesna, Demajo, Miroslav, Krtolica-Žikić, Koviljka, Krajnović, Milena M., Dimitrijević, Bogomir B., Konstantinovic, M., Baltic, Vladimir, Prtenjak, Gordana, Stojiljković, Bratislav, Breberina, Milan, Nešković-Konstantinović, Zora, Nikolic-Vukosavljevic, Dragica, "Elevated plasma TGF-beta(1) levels correlate with decreased survival of metastatic breast cancer patients" in Clinica Chimica Acta, 371, no. 1-2 (2006):191-193,
https://doi.org/10.1016/j.cca.2006.02.027 . .
50
54
56

Effect of EDTA on the inhibition of rat myometrial ecto-ATPase activity in the presence of heavy metal ions II. cadmium

Milošević, Maja; Banjac, Ana; Demajo, Miroslav; Horvat, Anica

(Society of Physical Chemists of Serbia, 2006)

TY  - CONF
AU  - Milošević, Maja
AU  - Banjac, Ana
AU  - Demajo, Miroslav
AU  - Horvat, Anica
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9428
AB  - The effects of ethylenediamine tetraacetic acid (EDTA) on the CdCl2 cell toxicity was examined on rat myometrium. Activity of plasma membrane ecto-ATPase, as modulator of purinergic signaling in the presence of increasing concentrations of cadmium salt and in the presence or absence of EDTA were studied. The EDTA, chelating cadmium ions decrease inhibitory cadmium potency by increasing half-maximum inhibitory activities of this ion.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
T1  - Effect of EDTA on the inhibition of rat myometrial ecto-ATPase activity in the presence of heavy metal ions II. cadmium
SP  - 398
EP  - 400
ER  - 
@conference{
author = "Milošević, Maja and Banjac, Ana and Demajo, Miroslav and Horvat, Anica",
year = "2006",
abstract = "The effects of ethylenediamine tetraacetic acid (EDTA) on the CdCl2 cell toxicity was examined on rat myometrium. Activity of plasma membrane ecto-ATPase, as modulator of purinergic signaling in the presence of increasing concentrations of cadmium salt and in the presence or absence of EDTA were studied. The EDTA, chelating cadmium ions decrease inhibitory cadmium potency by increasing half-maximum inhibitory activities of this ion.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry",
title = "Effect of EDTA on the inhibition of rat myometrial ecto-ATPase activity in the presence of heavy metal ions II. cadmium",
pages = "398-400"
}
Milošević, M., Banjac, A., Demajo, M.,& Horvat, A.. (2006). Effect of EDTA on the inhibition of rat myometrial ecto-ATPase activity in the presence of heavy metal ions II. cadmium. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 398-400.
Milošević M, Banjac A, Demajo M, Horvat A. Effect of EDTA on the inhibition of rat myometrial ecto-ATPase activity in the presence of heavy metal ions II. cadmium. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry. 2006;:398-400..
Milošević, Maja, Banjac, Ana, Demajo, Miroslav, Horvat, Anica, "Effect of EDTA on the inhibition of rat myometrial ecto-ATPase activity in the presence of heavy metal ions II. cadmium" in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry (2006):398-400.

Effect of EDTA on the inhibition of rat myometrial ecto-atpase activity in the presence of heavy metal ions I. Mercury

Milošević, Maja; Demajo, Miroslav; Horvat, Anica

(Society of Physical Chemists of Serbia, 2006)

TY  - CONF
AU  - Milošević, Maja
AU  - Demajo, Miroslav
AU  - Horvat, Anica
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9427
AB  - The effects of increasing concentrations of HgCl2 on rat uterine plasma membrane ecto-ATPase activity, in presence and absence of ethylenediamine tetra acetic acid (EDTA) were studied. The aim was to examine the ability of EDTA to prevent mercury induced inhibition of ecto-ATPase activity. Our results show that addition of 1mmol/l EDTA to the reaction mixture potentiates Hg2+ induced inhibition of enzyme activity. We may concluded that formation of the HgEDTA complex increased capacity of Hg2+ to inhibit enzyme activity.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
T1  - Effect of EDTA on the inhibition of rat myometrial ecto-atpase activity in the presence of heavy metal ions I. Mercury
SP  - 410
EP  - 412
ER  - 
@conference{
author = "Milošević, Maja and Demajo, Miroslav and Horvat, Anica",
year = "2006",
abstract = "The effects of increasing concentrations of HgCl2 on rat uterine plasma membrane ecto-ATPase activity, in presence and absence of ethylenediamine tetra acetic acid (EDTA) were studied. The aim was to examine the ability of EDTA to prevent mercury induced inhibition of ecto-ATPase activity. Our results show that addition of 1mmol/l EDTA to the reaction mixture potentiates Hg2+ induced inhibition of enzyme activity. We may concluded that formation of the HgEDTA complex increased capacity of Hg2+ to inhibit enzyme activity.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry",
title = "Effect of EDTA on the inhibition of rat myometrial ecto-atpase activity in the presence of heavy metal ions I. Mercury",
pages = "410-412"
}
Milošević, M., Demajo, M.,& Horvat, A.. (2006). Effect of EDTA on the inhibition of rat myometrial ecto-atpase activity in the presence of heavy metal ions I. Mercury. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 410-412.
Milošević M, Demajo M, Horvat A. Effect of EDTA on the inhibition of rat myometrial ecto-atpase activity in the presence of heavy metal ions I. Mercury. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry. 2006;:410-412..
Milošević, Maja, Demajo, Miroslav, Horvat, Anica, "Effect of EDTA on the inhibition of rat myometrial ecto-atpase activity in the presence of heavy metal ions I. Mercury" in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry (2006):410-412.

Selective inhibition of brain Na,K-ATPase by drugs

Horvat, Anica; Momić, Tatjana; Banjac, Ana; Petrović S.; Nikezic, G.; Demajo, Miroslav

(2006)

TY  - JOUR
AU  - Horvat, Anica
AU  - Momić, Tatjana
AU  - Banjac, Ana
AU  - Petrović S.
AU  - Nikezic, G.
AU  - Demajo, Miroslav
PY  - 2006
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3040
AB  - The effect of drugs from the class of cardiac (methyldigoxin, verapamil, propranolol), antiepileptic ( carbamazepine), sedative (diazepam) and antihistaminic (promethazine) drugs on Na,K-ATPase activity of plasma membranes was studied in rat brain synaptosomes. Methyldigoxin in a concentration of 0.1 mmol/l inhibits enzyme activity by 80%. Verapamil, propranolol and promethazine in concentrations of 20, 20 and 2 mmol/l respectively, entirely inhibit the ATPase activity. Carbamazepine and diazepam in concentrations of 0.02-60 mmol/l have no effect on the activity of this enzyme. According to the drug concentrations that inhibit 50% of enzyme activity (IC50), the potency can be listed in the following order: methyldigoxin GT GT promethazine GT verapamil GT = propranolol. From the inhibition of commercially available purified Na, K-ATPase isolated from porcine cerebral cortex in the presence of chosen drugs, as well as from kinetic studies on synaptosomal plasma membranes, it may be concluded that the drugs inhibit enzyme activity, partly by acting directly on the enzyme proteins. Propranolol, verapamil and promethazine inhibitions acted in an uncompetitive manner. The results suggest that these three drugs may contribute to neurological dysfunctions and indicate the necessity to take into consideration the side effects of the investigated drugs during the treatment of various pathological conditions.
T2  - Physiological Research
T1  - Selective inhibition of brain Na,K-ATPase by drugs
VL  - 55
IS  - 3
SP  - 325
EP  - 338
ER  - 
@article{
author = "Horvat, Anica and Momić, Tatjana and Banjac, Ana and Petrović S. and Nikezic, G. and Demajo, Miroslav",
year = "2006",
abstract = "The effect of drugs from the class of cardiac (methyldigoxin, verapamil, propranolol), antiepileptic ( carbamazepine), sedative (diazepam) and antihistaminic (promethazine) drugs on Na,K-ATPase activity of plasma membranes was studied in rat brain synaptosomes. Methyldigoxin in a concentration of 0.1 mmol/l inhibits enzyme activity by 80%. Verapamil, propranolol and promethazine in concentrations of 20, 20 and 2 mmol/l respectively, entirely inhibit the ATPase activity. Carbamazepine and diazepam in concentrations of 0.02-60 mmol/l have no effect on the activity of this enzyme. According to the drug concentrations that inhibit 50% of enzyme activity (IC50), the potency can be listed in the following order: methyldigoxin GT GT promethazine GT verapamil GT = propranolol. From the inhibition of commercially available purified Na, K-ATPase isolated from porcine cerebral cortex in the presence of chosen drugs, as well as from kinetic studies on synaptosomal plasma membranes, it may be concluded that the drugs inhibit enzyme activity, partly by acting directly on the enzyme proteins. Propranolol, verapamil and promethazine inhibitions acted in an uncompetitive manner. The results suggest that these three drugs may contribute to neurological dysfunctions and indicate the necessity to take into consideration the side effects of the investigated drugs during the treatment of various pathological conditions.",
journal = "Physiological Research",
title = "Selective inhibition of brain Na,K-ATPase by drugs",
volume = "55",
number = "3",
pages = "325-338"
}
Horvat, A., Momić, T., Banjac, A., Petrović S., Nikezic, G.,& Demajo, M.. (2006). Selective inhibition of brain Na,K-ATPase by drugs. in Physiological Research, 55(3), 325-338.
Horvat A, Momić T, Banjac A, Petrović S., Nikezic G, Demajo M. Selective inhibition of brain Na,K-ATPase by drugs. in Physiological Research. 2006;55(3):325-338..
Horvat, Anica, Momić, Tatjana, Banjac, Ana, Petrović S., Nikezic, G., Demajo, Miroslav, "Selective inhibition of brain Na,K-ATPase by drugs" in Physiological Research, 55, no. 3 (2006):325-338.
17

Inhibition of rat brain ecto-ATPase activity by various drugs

Horvat, Anica; Orlić, T; Banjac, Ana; Momić, Tatjana; Petrović, S; Demajo, Miroslav

(2006)

TY  - JOUR
AU  - Horvat, Anica
AU  - Orlić, T
AU  - Banjac, Ana
AU  - Momić, Tatjana
AU  - Petrović, S
AU  - Demajo, Miroslav
PY  - 2006
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3024
AB  - The in vitro effect of digoxin, verapamil, propranolol, carbamazepine, diazepam and promethazine were investigated on the ecto-ATPase activity of synaptosomal plasma membranes from the rat brain. ATP hydrolyzing activities of the enzyme were not affected by digoxin while the use of all other drugs resulted in significant and dose-dependent ihibition in ATP hydrolysis. According to values Of IC50 and K-iapp, the order of inhibitory potency of the drugs applied was: diazepam GT promethazine GT verapamil GT propranolol GT GT carbamazepine. Kinetic analysis of the nature of the ATPase inhibition revealed that it resulted from a direct action of drugs on the enzyme protein. The aim of the present study was to determine the potential neuromodulatory side effects of the drugs investigated. The results achieved indicated that all investigated drugs, except digoxin, may modulate neuronal activities via the purinergic receptors P2 by increasing extracellular concentrations of ATP as a consequence of inhibition of the ecto-ATPase activity. Our findings indicate that it may be useful to take into consideration the possible side effects of the investigated drugs, when they are used in treatment of different pathologies, particularly in the treatment of epilepsy by carbamazepine and diazepam.
T2  - General Physiology and Biophysics
T1  - Inhibition of rat brain ecto-ATPase activity by various drugs
VL  - 25
IS  - 1
SP  - 91
EP  - 105
ER  - 
@article{
author = "Horvat, Anica and Orlić, T and Banjac, Ana and Momić, Tatjana and Petrović, S and Demajo, Miroslav",
year = "2006",
abstract = "The in vitro effect of digoxin, verapamil, propranolol, carbamazepine, diazepam and promethazine were investigated on the ecto-ATPase activity of synaptosomal plasma membranes from the rat brain. ATP hydrolyzing activities of the enzyme were not affected by digoxin while the use of all other drugs resulted in significant and dose-dependent ihibition in ATP hydrolysis. According to values Of IC50 and K-iapp, the order of inhibitory potency of the drugs applied was: diazepam GT promethazine GT verapamil GT propranolol GT GT carbamazepine. Kinetic analysis of the nature of the ATPase inhibition revealed that it resulted from a direct action of drugs on the enzyme protein. The aim of the present study was to determine the potential neuromodulatory side effects of the drugs investigated. The results achieved indicated that all investigated drugs, except digoxin, may modulate neuronal activities via the purinergic receptors P2 by increasing extracellular concentrations of ATP as a consequence of inhibition of the ecto-ATPase activity. Our findings indicate that it may be useful to take into consideration the possible side effects of the investigated drugs, when they are used in treatment of different pathologies, particularly in the treatment of epilepsy by carbamazepine and diazepam.",
journal = "General Physiology and Biophysics",
title = "Inhibition of rat brain ecto-ATPase activity by various drugs",
volume = "25",
number = "1",
pages = "91-105"
}
Horvat, A., Orlić, T., Banjac, A., Momić, T., Petrović, S.,& Demajo, M.. (2006). Inhibition of rat brain ecto-ATPase activity by various drugs. in General Physiology and Biophysics, 25(1), 91-105.
Horvat A, Orlić T, Banjac A, Momić T, Petrović S, Demajo M. Inhibition of rat brain ecto-ATPase activity by various drugs. in General Physiology and Biophysics. 2006;25(1):91-105..
Horvat, Anica, Orlić, T, Banjac, Ana, Momić, Tatjana, Petrović, S, Demajo, Miroslav, "Inhibition of rat brain ecto-ATPase activity by various drugs" in General Physiology and Biophysics, 25, no. 1 (2006):91-105.
10

Early effects of ionizing radiation on rat brain NTPDase activity

Horvat, Anica; Petrovic, S; Milošević, Maja; Stanojević, Ivana; Demajo, Miroslav

(2005)

TY  - CONF
AU  - Horvat, Anica
AU  - Petrovic, S
AU  - Milošević, Maja
AU  - Stanojević, Ivana
AU  - Demajo, Miroslav
PY  - 2005
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6563
C3  - FEBS Journal
T1  - Early effects of ionizing radiation on rat brain NTPDase activity
VL  - 272
SP  - 190
EP  - 190
ER  - 
@conference{
author = "Horvat, Anica and Petrovic, S and Milošević, Maja and Stanojević, Ivana and Demajo, Miroslav",
year = "2005",
journal = "FEBS Journal",
title = "Early effects of ionizing radiation on rat brain NTPDase activity",
volume = "272",
pages = "190-190"
}
Horvat, A., Petrovic, S., Milošević, M., Stanojević, I.,& Demajo, M.. (2005). Early effects of ionizing radiation on rat brain NTPDase activity. in FEBS Journal, 272, 190-190.
Horvat A, Petrovic S, Milošević M, Stanojević I, Demajo M. Early effects of ionizing radiation on rat brain NTPDase activity. in FEBS Journal. 2005;272:190-190..
Horvat, Anica, Petrovic, S, Milošević, Maja, Stanojević, Ivana, Demajo, Miroslav, "Early effects of ionizing radiation on rat brain NTPDase activity" in FEBS Journal, 272 (2005):190-190.

Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries

Milošević, Maja; Petrovic, S; Demajo, Miroslav; Horvat, Anica

(2005)

TY  - JOUR
AU  - Milošević, Maja
AU  - Petrovic, S
AU  - Demajo, Miroslav
AU  - Horvat, Anica
PY  - 2005
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6556
AB  - The in vitro effects of cadmium and mercury were investigated on the Mg2+-ATPase activity of plasma membranes from the rat ovary and uterus. ATP hydrolyzing activities were significant and dose-dependent-inhibited in both plasma membrane preparations by both metals. According to the IC50 and apparent K-i, Cd2+ was most potent in the ovary, while Hg2+ was most potent in the uterus. In ovaries and uterus,Cd2+ inhibits competitively, while Hg2+ inhibits noncompetitively in both organs. The observed inhibition was a consequence of direct action of the chosen metal ions on the enzyme protein and by decreasing ATP hydrolysis, Hg2+ and Cd2+ may affect mammalian fertility.
T2  - Annals of the New York Academy of Sciences
T1  - Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries
VL  - 1048
SP  - 445
EP  - 448
DO  - 10.1196/annals.1342.061
ER  - 
@article{
author = "Milošević, Maja and Petrovic, S and Demajo, Miroslav and Horvat, Anica",
year = "2005",
abstract = "The in vitro effects of cadmium and mercury were investigated on the Mg2+-ATPase activity of plasma membranes from the rat ovary and uterus. ATP hydrolyzing activities were significant and dose-dependent-inhibited in both plasma membrane preparations by both metals. According to the IC50 and apparent K-i, Cd2+ was most potent in the ovary, while Hg2+ was most potent in the uterus. In ovaries and uterus,Cd2+ inhibits competitively, while Hg2+ inhibits noncompetitively in both organs. The observed inhibition was a consequence of direct action of the chosen metal ions on the enzyme protein and by decreasing ATP hydrolysis, Hg2+ and Cd2+ may affect mammalian fertility.",
journal = "Annals of the New York Academy of Sciences",
title = "Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries",
volume = "1048",
pages = "445-448",
doi = "10.1196/annals.1342.061"
}
Milošević, M., Petrovic, S., Demajo, M.,& Horvat, A.. (2005). Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries. in Annals of the New York Academy of Sciences, 1048, 445-448.
https://doi.org/10.1196/annals.1342.061
Milošević M, Petrovic S, Demajo M, Horvat A. Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries. in Annals of the New York Academy of Sciences. 2005;1048:445-448.
doi:10.1196/annals.1342.061 .
Milošević, Maja, Petrovic, S, Demajo, Miroslav, Horvat, Anica, "Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries" in Annals of the New York Academy of Sciences, 1048 (2005):445-448,
https://doi.org/10.1196/annals.1342.061 . .
5
4
5

Estradiol affects calcium transport across mitochondrial membrane in different brain regions

Petrovic, SA; Demajo, Miroslav; Horvat, Anica

(2005)

TY  - JOUR
AU  - Petrovic, SA
AU  - Demajo, Miroslav
AU  - Horvat, Anica
PY  - 2005
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6551
AB  - The in vitro effect of estradiol on flux of Ca2+ in the synaptosomal mitochondria from nucleus caudatus and hippocampus of chronically ovariectomized female rats was examined. No effect of estradiol on Ca2+, influx through ruthenium red-sensitive channels was found. Estradiol, at a concentration of 0.05-5 nmol/L for nucleus caudatus and 0.5-5 nmol/L for the hippocampus, decreased Na-dependent Ca2+ efflux about 25%.
T2  - Annals of the New York Academy of Sciences
T1  - Estradiol affects calcium transport across mitochondrial membrane in different brain regions
VL  - 1048
SP  - 341
EP  - 343
DO  - 10.1196/annals.1342.032
ER  - 
@article{
author = "Petrovic, SA and Demajo, Miroslav and Horvat, Anica",
year = "2005",
abstract = "The in vitro effect of estradiol on flux of Ca2+ in the synaptosomal mitochondria from nucleus caudatus and hippocampus of chronically ovariectomized female rats was examined. No effect of estradiol on Ca2+, influx through ruthenium red-sensitive channels was found. Estradiol, at a concentration of 0.05-5 nmol/L for nucleus caudatus and 0.5-5 nmol/L for the hippocampus, decreased Na-dependent Ca2+ efflux about 25%.",
journal = "Annals of the New York Academy of Sciences",
title = "Estradiol affects calcium transport across mitochondrial membrane in different brain regions",
volume = "1048",
pages = "341-343",
doi = "10.1196/annals.1342.032"
}
Petrovic, S., Demajo, M.,& Horvat, A.. (2005). Estradiol affects calcium transport across mitochondrial membrane in different brain regions. in Annals of the New York Academy of Sciences, 1048, 341-343.
https://doi.org/10.1196/annals.1342.032
Petrovic S, Demajo M, Horvat A. Estradiol affects calcium transport across mitochondrial membrane in different brain regions. in Annals of the New York Academy of Sciences. 2005;1048:341-343.
doi:10.1196/annals.1342.032 .
Petrovic, SA, Demajo, Miroslav, Horvat, Anica, "Estradiol affects calcium transport across mitochondrial membrane in different brain regions" in Annals of the New York Academy of Sciences, 1048 (2005):341-343,
https://doi.org/10.1196/annals.1342.032 . .
6
5
7

Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin

Korićanac, Lela; Todorović, Danijela V.; Popović, Nataša M.; Demajo, Miroslav; Ruždijić, Sabera; Ristić-Fira, Aleksandra

(2004)

TY  - JOUR
AU  - Korićanac, Lela
AU  - Todorović, Danijela V.
AU  - Popović, Nataša M.
AU  - Demajo, Miroslav
AU  - Ruždijić, Sabera
AU  - Ristić-Fira, Aleksandra
PY  - 2004
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6500
AB  - Novel antineoplastic agents, 8-chloroadenosine 3,5-monophosphate (8-Cl-cAMP) and tiazofurin (TR), have been shown to be effective against different malignant cells. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, and apoptosis. The aim of this work was the in vitro study of either 8-Cl-cAMP or TR effects on B16/F10 and B16/C3 mouse melanoma cell growth and cell death. Significant cell growth inhibition was obtained after the application of 8-Cl-cAMP or TR. The presence and number of apoptotic cells was evaluated using agarose gel electrophoresis and flow cytometry. The number of apoptotic nuclei, after treatment with antineoplastic agents, did not significantly change in B16/F10 cells, although it did show a significant increase in B16/C3 cells. The expression of c-myc did not significantly change in B16/F10 cells after treatment with 8-Cl-cAMP or TR. The same results were obtained in B16/C3 cells after treatment with 8-Cl-cAMP. The level of c-myc expression showed a significant increase in B16/C3 cells after treatment with TR. Concerning the effects that the analyzed agents exhibited on melanoma cells and other cancer cells, further preclinical studies of these drugs will potentially lead to better understanding of the molecular mechanisms of their action and finally more efficient therapeutic approaches to malignant diseases.
T2  - Annals of the New York Academy of Sciences
T1  - Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin
VL  - 1030
SP  - 384
EP  - 392
DO  - 10.1196/annals.1329.048
ER  - 
@article{
author = "Korićanac, Lela and Todorović, Danijela V. and Popović, Nataša M. and Demajo, Miroslav and Ruždijić, Sabera and Ristić-Fira, Aleksandra",
year = "2004",
abstract = "Novel antineoplastic agents, 8-chloroadenosine 3,5-monophosphate (8-Cl-cAMP) and tiazofurin (TR), have been shown to be effective against different malignant cells. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, and apoptosis. The aim of this work was the in vitro study of either 8-Cl-cAMP or TR effects on B16/F10 and B16/C3 mouse melanoma cell growth and cell death. Significant cell growth inhibition was obtained after the application of 8-Cl-cAMP or TR. The presence and number of apoptotic cells was evaluated using agarose gel electrophoresis and flow cytometry. The number of apoptotic nuclei, after treatment with antineoplastic agents, did not significantly change in B16/F10 cells, although it did show a significant increase in B16/C3 cells. The expression of c-myc did not significantly change in B16/F10 cells after treatment with 8-Cl-cAMP or TR. The same results were obtained in B16/C3 cells after treatment with 8-Cl-cAMP. The level of c-myc expression showed a significant increase in B16/C3 cells after treatment with TR. Concerning the effects that the analyzed agents exhibited on melanoma cells and other cancer cells, further preclinical studies of these drugs will potentially lead to better understanding of the molecular mechanisms of their action and finally more efficient therapeutic approaches to malignant diseases.",
journal = "Annals of the New York Academy of Sciences",
title = "Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin",
volume = "1030",
pages = "384-392",
doi = "10.1196/annals.1329.048"
}
Korićanac, L., Todorović, D. V., Popović, N. M., Demajo, M., Ruždijić, S.,& Ristić-Fira, A.. (2004). Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin. in Annals of the New York Academy of Sciences, 1030, 384-392.
https://doi.org/10.1196/annals.1329.048
Korićanac L, Todorović DV, Popović NM, Demajo M, Ruždijić S, Ristić-Fira A. Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin. in Annals of the New York Academy of Sciences. 2004;1030:384-392.
doi:10.1196/annals.1329.048 .
Korićanac, Lela, Todorović, Danijela V., Popović, Nataša M., Demajo, Miroslav, Ruždijić, Sabera, Ristić-Fira, Aleksandra, "Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin" in Annals of the New York Academy of Sciences, 1030 (2004):384-392,
https://doi.org/10.1196/annals.1329.048 . .
1
2
3

Plasma TGF-beta 1-related survival of postmenopausal metastatic breast cancer patients

Nikolic-Vukosavljevic, D; Todorović-Raković, N; Demajo, Miroslav; Ivanović, Vesna; Neskovic, B; Markicevic, M; Nešković-Konstantinović, Zora

(2004)

TY  - JOUR
AU  - Nikolic-Vukosavljevic, D
AU  - Todorović-Raković, N
AU  - Demajo, Miroslav
AU  - Ivanović, Vesna
AU  - Neskovic, B
AU  - Markicevic, M
AU  - Nešković-Konstantinović, Zora
PY  - 2004
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/2869
AB  - A pilot study was conducted to assess whether plasma levels of transforming growth factor-beta 1 (TGF-beta 1) might facilitate biological subgrouping of postmenopausal metastatic breast cancer patients, and, accordingly, its applicability in clinical oncology. This study included 29 postmenopausal metastatic breast cancer patients. Plasma TGF-beta 1 levels were detected by enzyme-linked immunosorbent assay (ELISA). Estrogen and progesterone receptors were assayed by radioligand binding, in accordance with the recommendation of the EORTC. Concentrations of 17-beta estradiol were determined by using ELISA-microwell method (DIALAB). Overall survival was followed for 24 months for each individual patient. Stratification of the patients by ER/PR status showed that 14 patients with estrogen receptor-negative, progesterone receptor-negative carcinomas displayed a statistically significant increase in plasma TGF-beta 1 levels when compared to plasma TGF-beta 1 levels of 6 patients with ER-positive, PR-positive carcinomas (P=0.04). In this study, 7 out of 14 patients with negative receptors status had no plasma TGF-beta 1 values overlapping with patients having positive receptors status. The TGF-beta 1 cut-off value was defined as the highest plasma TGF-beta 1 level of ER-positive, PR-positive patients: 3.28 ng/ml. This plasma TGF-beta 1 cut-off value defined low-risk subgroup of 19 patients (! 3.28 ng/ml) and high-risk subgroup of 10 patients ( GT 3.28 ng/ml) (P=0.047). Plasma TGF-beta 1-related survival was independent of the classical prognostic factors of metastatic breast cancer. Accordingly, a clinical significance of elevated plasma TGF-beta 1 levels may be suggested.
T2  - Clinical and Experimental Metastasis
T1  - Plasma TGF-beta 1-related survival of postmenopausal metastatic breast cancer patients
VL  - 21
IS  - 7
SP  - 581
EP  - 585
ER  - 
@article{
author = "Nikolic-Vukosavljevic, D and Todorović-Raković, N and Demajo, Miroslav and Ivanović, Vesna and Neskovic, B and Markicevic, M and Nešković-Konstantinović, Zora",
year = "2004",
abstract = "A pilot study was conducted to assess whether plasma levels of transforming growth factor-beta 1 (TGF-beta 1) might facilitate biological subgrouping of postmenopausal metastatic breast cancer patients, and, accordingly, its applicability in clinical oncology. This study included 29 postmenopausal metastatic breast cancer patients. Plasma TGF-beta 1 levels were detected by enzyme-linked immunosorbent assay (ELISA). Estrogen and progesterone receptors were assayed by radioligand binding, in accordance with the recommendation of the EORTC. Concentrations of 17-beta estradiol were determined by using ELISA-microwell method (DIALAB). Overall survival was followed for 24 months for each individual patient. Stratification of the patients by ER/PR status showed that 14 patients with estrogen receptor-negative, progesterone receptor-negative carcinomas displayed a statistically significant increase in plasma TGF-beta 1 levels when compared to plasma TGF-beta 1 levels of 6 patients with ER-positive, PR-positive carcinomas (P=0.04). In this study, 7 out of 14 patients with negative receptors status had no plasma TGF-beta 1 values overlapping with patients having positive receptors status. The TGF-beta 1 cut-off value was defined as the highest plasma TGF-beta 1 level of ER-positive, PR-positive patients: 3.28 ng/ml. This plasma TGF-beta 1 cut-off value defined low-risk subgroup of 19 patients (! 3.28 ng/ml) and high-risk subgroup of 10 patients ( GT 3.28 ng/ml) (P=0.047). Plasma TGF-beta 1-related survival was independent of the classical prognostic factors of metastatic breast cancer. Accordingly, a clinical significance of elevated plasma TGF-beta 1 levels may be suggested.",
journal = "Clinical and Experimental Metastasis",
title = "Plasma TGF-beta 1-related survival of postmenopausal metastatic breast cancer patients",
volume = "21",
number = "7",
pages = "581-585"
}
Nikolic-Vukosavljevic, D., Todorović-Raković, N., Demajo, M., Ivanović, V., Neskovic, B., Markicevic, M.,& Nešković-Konstantinović, Z.. (2004). Plasma TGF-beta 1-related survival of postmenopausal metastatic breast cancer patients. in Clinical and Experimental Metastasis, 21(7), 581-585.
Nikolic-Vukosavljevic D, Todorović-Raković N, Demajo M, Ivanović V, Neskovic B, Markicevic M, Nešković-Konstantinović Z. Plasma TGF-beta 1-related survival of postmenopausal metastatic breast cancer patients. in Clinical and Experimental Metastasis. 2004;21(7):581-585..
Nikolic-Vukosavljevic, D, Todorović-Raković, N, Demajo, Miroslav, Ivanović, Vesna, Neskovic, B, Markicevic, M, Nešković-Konstantinović, Zora, "Plasma TGF-beta 1-related survival of postmenopausal metastatic breast cancer patients" in Clinical and Experimental Metastasis, 21, no. 7 (2004):581-585.
15

Localization of recognition site between transforming growth factor-beta(1) (TGF-beta(1)) and TGF beta receptor type II: possible implications in breast cancer

Ivanović, Vesna; Demajo, Miroslav; Todorović-Raković, N; Nikolic-Vukosavljevic, D; Nešković-Konstantinović, Zora; Krtolica-Žikić, Koviljka; Veljković, Veljko; Prljić, Jelena; Dimitrijević, Bogomir B.

(2004)

TY  - JOUR
AU  - Ivanović, Vesna
AU  - Demajo, Miroslav
AU  - Todorović-Raković, N
AU  - Nikolic-Vukosavljevic, D
AU  - Nešković-Konstantinović, Zora
AU  - Krtolica-Žikić, Koviljka
AU  - Veljković, Veljko
AU  - Prljić, Jelena
AU  - Dimitrijević, Bogomir B.
PY  - 2004
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/2769
AB  - Although overexpression of TGF-beta(1) protein has been demonstrated in advanced breast cancer(BC) patients, as well as in other solid tumours, the molecular mechanism of this process remains obscure. This paper proposes that a genetic/epigenetic alteration might occur in the TGF-beta(1) gene, within the region coding for the recognition site with TGF(beta) receptor type II, leading to a disruption of the ligand-receptor interaction and triggering the TGF-beta(1) cascade-related BC progression. To establish the operational framework for this hypothesis, in the present study, this recognition site was identified by the Informational Spectrum Method (ISM) to comprise two TGF-beta(1) peptides (positions 47-66 as and 83-112 aa) and one receptor peptide at positions 112-151 as of the extracellular domain of the receptor (TbetaRII(M)). The TbetaRII(M) locus was further evaluated by ISM-derived deletion analysis of the TbetaRII sequences. To provide experimental support for the proposed model, a pilot study of plasma TGF-beta(1) analysis was performed in advanced BC patients (n = 8). Two commercial ELISA assays, one with specific alphaTGF-beta(1) MAb (MAb) and other with TbetaRII(M) as the immobilized phase, revealed pronounced differences in the pattern of plasma TGF-beta(1) elevation. In MAb-profile, the TGF-beta(1) increase was detected in 7 of 8 patients, whereas analogous TbetaRII(M)-profile revealed the elevation in 3 of 8 patients, taking a 50% of maximal elevation as the cut-off value. These findings are consistent with the proposed aberration of TGF-beta(1) ligand within the TbetaRII recognition site. Summarizing, this model system is a good starting point for further genetic studies, particularly on genetic/epigenetic alterations of sequences involved in TGF-beta(1) and TbetaRII(M) interaction, with putative prognostic value for breast cancer. (C) 2004 Elsevier Ltd. All rights reserved.
T2  - Medical Hypotheses
T1  - Localization of recognition site between transforming growth factor-beta(1) (TGF-beta(1)) and TGF beta receptor type II: possible implications in breast cancer
VL  - 62
IS  - 5
SP  - 727
EP  - 732
DO  - 10.1016/j.mehy.2003.11.027
ER  - 
@article{
author = "Ivanović, Vesna and Demajo, Miroslav and Todorović-Raković, N and Nikolic-Vukosavljevic, D and Nešković-Konstantinović, Zora and Krtolica-Žikić, Koviljka and Veljković, Veljko and Prljić, Jelena and Dimitrijević, Bogomir B.",
year = "2004",
abstract = "Although overexpression of TGF-beta(1) protein has been demonstrated in advanced breast cancer(BC) patients, as well as in other solid tumours, the molecular mechanism of this process remains obscure. This paper proposes that a genetic/epigenetic alteration might occur in the TGF-beta(1) gene, within the region coding for the recognition site with TGF(beta) receptor type II, leading to a disruption of the ligand-receptor interaction and triggering the TGF-beta(1) cascade-related BC progression. To establish the operational framework for this hypothesis, in the present study, this recognition site was identified by the Informational Spectrum Method (ISM) to comprise two TGF-beta(1) peptides (positions 47-66 as and 83-112 aa) and one receptor peptide at positions 112-151 as of the extracellular domain of the receptor (TbetaRII(M)). The TbetaRII(M) locus was further evaluated by ISM-derived deletion analysis of the TbetaRII sequences. To provide experimental support for the proposed model, a pilot study of plasma TGF-beta(1) analysis was performed in advanced BC patients (n = 8). Two commercial ELISA assays, one with specific alphaTGF-beta(1) MAb (MAb) and other with TbetaRII(M) as the immobilized phase, revealed pronounced differences in the pattern of plasma TGF-beta(1) elevation. In MAb-profile, the TGF-beta(1) increase was detected in 7 of 8 patients, whereas analogous TbetaRII(M)-profile revealed the elevation in 3 of 8 patients, taking a 50% of maximal elevation as the cut-off value. These findings are consistent with the proposed aberration of TGF-beta(1) ligand within the TbetaRII recognition site. Summarizing, this model system is a good starting point for further genetic studies, particularly on genetic/epigenetic alterations of sequences involved in TGF-beta(1) and TbetaRII(M) interaction, with putative prognostic value for breast cancer. (C) 2004 Elsevier Ltd. All rights reserved.",
journal = "Medical Hypotheses",
title = "Localization of recognition site between transforming growth factor-beta(1) (TGF-beta(1)) and TGF beta receptor type II: possible implications in breast cancer",
volume = "62",
number = "5",
pages = "727-732",
doi = "10.1016/j.mehy.2003.11.027"
}
Ivanović, V., Demajo, M., Todorović-Raković, N., Nikolic-Vukosavljevic, D., Nešković-Konstantinović, Z., Krtolica-Žikić, K., Veljković, V., Prljić, J.,& Dimitrijević, B. B.. (2004). Localization of recognition site between transforming growth factor-beta(1) (TGF-beta(1)) and TGF beta receptor type II: possible implications in breast cancer. in Medical Hypotheses, 62(5), 727-732.
https://doi.org/10.1016/j.mehy.2003.11.027
Ivanović V, Demajo M, Todorović-Raković N, Nikolic-Vukosavljevic D, Nešković-Konstantinović Z, Krtolica-Žikić K, Veljković V, Prljić J, Dimitrijević BB. Localization of recognition site between transforming growth factor-beta(1) (TGF-beta(1)) and TGF beta receptor type II: possible implications in breast cancer. in Medical Hypotheses. 2004;62(5):727-732.
doi:10.1016/j.mehy.2003.11.027 .
Ivanović, Vesna, Demajo, Miroslav, Todorović-Raković, N, Nikolic-Vukosavljevic, D, Nešković-Konstantinović, Zora, Krtolica-Žikić, Koviljka, Veljković, Veljko, Prljić, Jelena, Dimitrijević, Bogomir B., "Localization of recognition site between transforming growth factor-beta(1) (TGF-beta(1)) and TGF beta receptor type II: possible implications in breast cancer" in Medical Hypotheses, 62, no. 5 (2004):727-732,
https://doi.org/10.1016/j.mehy.2003.11.027 . .
3
4
5

Transforming growth factor-beta1 and steroid receptor status in breast cancer

Todorović-Raković, N.; Ivanović, Vesna; Demajo, Miroslav; Nešković-Konstantinović, Zora; Nikolic-Vukosavljevic, D.

(2004)

TY  - CONF
AU  - Todorović-Raković, N.
AU  - Ivanović, Vesna
AU  - Demajo, Miroslav
AU  - Nešković-Konstantinović, Zora
AU  - Nikolic-Vukosavljevic, D.
PY  - 2004
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/2718
C3  - European Journal of Cancer Supplements / EJC Supplements
T1  - Transforming growth factor-beta1 and steroid receptor status in breast cancer
VL  - 2
IS  - 3
SP  - 105
EP  - 105
DO  - 10.1016/S1359-6349(04)90778-1
ER  - 
@conference{
author = "Todorović-Raković, N. and Ivanović, Vesna and Demajo, Miroslav and Nešković-Konstantinović, Zora and Nikolic-Vukosavljevic, D.",
year = "2004",
journal = "European Journal of Cancer Supplements / EJC Supplements",
title = "Transforming growth factor-beta1 and steroid receptor status in breast cancer",
volume = "2",
number = "3",
pages = "105-105",
doi = "10.1016/S1359-6349(04)90778-1"
}
Todorović-Raković, N., Ivanović, V., Demajo, M., Nešković-Konstantinović, Z.,& Nikolic-Vukosavljevic, D.. (2004). Transforming growth factor-beta1 and steroid receptor status in breast cancer. in European Journal of Cancer Supplements / EJC Supplements, 2(3), 105-105.
https://doi.org/10.1016/S1359-6349(04)90778-1
Todorović-Raković N, Ivanović V, Demajo M, Nešković-Konstantinović Z, Nikolic-Vukosavljevic D. Transforming growth factor-beta1 and steroid receptor status in breast cancer. in European Journal of Cancer Supplements / EJC Supplements. 2004;2(3):105-105.
doi:10.1016/S1359-6349(04)90778-1 .
Todorović-Raković, N., Ivanović, Vesna, Demajo, Miroslav, Nešković-Konstantinović, Zora, Nikolic-Vukosavljevic, D., "Transforming growth factor-beta1 and steroid receptor status in breast cancer" in European Journal of Cancer Supplements / EJC Supplements, 2, no. 3 (2004):105-105,
https://doi.org/10.1016/S1359-6349(04)90778-1 . .