Čolović, Mirjana B.

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Authority KeyName Variants
orcid::0000-0001-5037-9985
  • Čolović, Mirjana B. (56)
Projects
Studies of enzyme interactions with toxic and pharmacologically active molecules Istraživanje mehanizma interakcija biološki aktivnih jedinjenja sa biomolekulima
The effects of homocysteine and homocysteine-related compounds on cardiovascular system: role of gaseous transmitters No, H2S and CO CMST COST Action [CM1203 (PoCheMoN)]
Electroconducting and redox-active polymers and oligomers: synthesis, structure, properties and applications Basic and Clinical Pharmacological research of mechanisms of action and drug interactions in nervous and cardiovascular system
The development of animal models of epilepsy and testing convulsive and anticonvulsive substances AIRC [IG-12085], Beneficentia Stiftung Vaduz
Beneficentia Stiftung (Vaduz), ITT (Istituto Toscano Tumori), Fondazione Cassa Risparmio Firenze (CRF), AIRC [IG-16049], AIRC-FIRC (Fondazione Italiana per la Ricerca sul Cancro) [18044] Bilateral project Serbia–Germany [451-03-01038/2015-09/16, DAAD-PPP]
Campus France for a PHC support ["Pavle Savić" 23643QC] Campus France for a Prestige grant
China Scholarship Council Chinese Science of Council
CMST COST Action [CM1203] COST Action [CA16113]
COST action CM1203 Polyoxometalate Chemistry for Molecular Nanoscience (PoCheMoN), COST-STSM-ECOST-STSM-CM1203-030416-072554 CSC, Wuhan Applied Basic Research Program [2014010101010020]
Generalitat de Catalunya [2017SGR629] German Research Council (DFG) [KO-2288/16-1]
German Research Council (DFG) [KO-2288/20-1] German Research Foundation (DFG) [DEG-KO-2288/16-1]
German Research Foundation (DFG) [DFG-KO-2288/20-1] Hungarian National Research, Development and Innovation Office - NKFIH [NKFI NN 128368]
ICREA The membranes as sites of interaction between the intracellular and apoplastic environments: studies of the bioenergetics and signaling using biophysical and biochemical techniques.
Biomarkers of organ damage and dysfunction Significance of early diagnosis of obstructive sleep apnea syndrome in professional drivers operating motor vehicles
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM)

Author's Bibliography

A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition

Bondžić, Aleksandra; Lazarević-Pašti, Tamara; Leskovac, Andreja; Petrović, Sandra; Čolović, Mirjana B.; Parac Vogt, Tatjana N.; Janjić, Goran V.

(2020)

TY  - JOUR
AU  - Bondžić, Aleksandra
AU  - Lazarević-Pašti, Tamara
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Čolović, Mirjana B.
AU  - Parac Vogt, Tatjana N.
AU  - Janjić, Goran V.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9028
AB  - Acetylcholinesterase (AChE) inhibitors are important in the treatment of neurodegenerative diseases. Two inhibitors,12-tungstosilicic acid (WSiA) and 12-tungstophosphoric acid (WPA), which have polyoxometalate(POM) type structure, have been shown to inhibit AChE activity in nM concentration. Circular dichroism andtryptophan fluorescence spectroscopy demonstrated that the AChE inhibition was not accompanied by significantchanges in the secondary structure of the enzyme. The molecular docking approach has revealed a newallosteric binding site, termed β-allosteric site (β-AS), which is considered responsible for the inhibition of AChEby POMs. To the best of our knowledge, this is the first study reporting a new allosteric site that is consideredresponsible for AChE inhibition by voluminous and negatively charged molecules such as POMs. The selectedPOMs were further subjected to genotoxicity testing using human peripheral blood cells as a model system. Itwas shown that WSiA and WPA induced a mild cytostatic but not genotoxic effects in human lymphocytes, whichindicates their potential to be used as medicinal drugs. The identification of non-toxic compounds capable ofbinding to an allosteric site that so far has not been considered responsible for enzyme inhibition could befundamental for the development of new drug design strategies and the discovery of more efficient AChEmodulators.
T2  - European Journal of Pharmaceutical Sciences
T1  - A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition
VL  - 151
SP  - 105376
DO  - 10.1016/j.ejps.2020.105376
ER  - 
@article{
author = "Bondžić, Aleksandra and Lazarević-Pašti, Tamara and Leskovac, Andreja and Petrović, Sandra and Čolović, Mirjana B. and Parac Vogt, Tatjana N. and Janjić, Goran V.",
year = "2020",
abstract = "Acetylcholinesterase (AChE) inhibitors are important in the treatment of neurodegenerative diseases. Two inhibitors,12-tungstosilicic acid (WSiA) and 12-tungstophosphoric acid (WPA), which have polyoxometalate(POM) type structure, have been shown to inhibit AChE activity in nM concentration. Circular dichroism andtryptophan fluorescence spectroscopy demonstrated that the AChE inhibition was not accompanied by significantchanges in the secondary structure of the enzyme. The molecular docking approach has revealed a newallosteric binding site, termed β-allosteric site (β-AS), which is considered responsible for the inhibition of AChEby POMs. To the best of our knowledge, this is the first study reporting a new allosteric site that is consideredresponsible for AChE inhibition by voluminous and negatively charged molecules such as POMs. The selectedPOMs were further subjected to genotoxicity testing using human peripheral blood cells as a model system. Itwas shown that WSiA and WPA induced a mild cytostatic but not genotoxic effects in human lymphocytes, whichindicates their potential to be used as medicinal drugs. The identification of non-toxic compounds capable ofbinding to an allosteric site that so far has not been considered responsible for enzyme inhibition could befundamental for the development of new drug design strategies and the discovery of more efficient AChEmodulators.",
journal = "European Journal of Pharmaceutical Sciences",
title = "A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition",
volume = "151",
pages = "105376",
doi = "10.1016/j.ejps.2020.105376"
}
Bondžić, A., Lazarević-Pašti, T., Leskovac, A., Petrović, S., Čolović, M. B., Parac Vogt, T. N.,& Janjić, G. V.. (2020). A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition. in European Journal of Pharmaceutical Sciences, 151, 105376.
https://doi.org/10.1016/j.ejps.2020.105376
Bondžić A, Lazarević-Pašti T, Leskovac A, Petrović S, Čolović MB, Parac Vogt TN, Janjić GV. A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition. in European Journal of Pharmaceutical Sciences. 2020;151:105376.
doi:10.1016/j.ejps.2020.105376 .
Bondžić, Aleksandra, Lazarević-Pašti, Tamara, Leskovac, Andreja, Petrović, Sandra, Čolović, Mirjana B., Parac Vogt, Tatjana N., Janjić, Goran V., "A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition" in European Journal of Pharmaceutical Sciences, 151 (2020):105376,
https://doi.org/10.1016/j.ejps.2020.105376 . .
4
3

In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system

Dinčić, Marko; Čolović, Mirjana B.; Sarić Matutinović, Marija; Ćetković, Mila; Kravić-Stevović, Tamara K.; Mougharbel, Ali S.; Todorović, Jasna; Ignjatović, Svetlana; Radosavljević, Branimir; Milisavljević, Milan; Kortz, Ulrich; Krstić, Danijela Z.

(2020)

TY  - JOUR
AU  - Dinčić, Marko
AU  - Čolović, Mirjana B.
AU  - Sarić Matutinović, Marija
AU  - Ćetković, Mila
AU  - Kravić-Stevović, Tamara K.
AU  - Mougharbel, Ali S.
AU  - Todorović, Jasna
AU  - Ignjatović, Svetlana
AU  - Radosavljević, Branimir
AU  - Milisavljević, Milan
AU  - Kortz, Ulrich
AU  - Krstić, Danijela Z.
PY  - 2020
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8479
AB  - In this study, thein vivohypoglycemic effect of a donut-shaped polyanion salt (NH4)14[Na@P5W30O110]$31H2O{NaP5W30} and its Ag-containing derivative K14[Ag@P5W30O110]$22H2O$6KCl {AgP5W30}, as wellas their hepatotoxicity and nephrotoxicity, was evaluated. In the screening hypoglycemic study,Wistaralbinorats with streptozotocin induced diabetes were treated intraperitoneally with three single doses (5,10, and 20 mg per kg per b.w.) of both investigated polyoxotungstates. The blood glucose levels,measured before and after 2, 4 and 6 h polyoxotungstate application, showed that both studiedcompounds induced the most pronounced and time dependent glucose lowering effects at the doses of20 mg kg1. Thus, daily doses of 20 mg kg1were administered toWistar albinorats orally for 14 days infurther toxicity examinations. The serum glucose concentration and biochemical parameters of kidneyand liver function, as well as a histopathological analysis of kidney and liver tissues were evaluated 14days after the polyoxotungstate administration. Both investigated compounds did not induce statisticallysignificant alterations of the serum glucose and uric acid concentrations, as well as some of the liverfunction markers (serum alanine and aspartate aminotransferases, and alkaline phosphatase activities).However, the significant decrease in serum total protein and albumin concentrations and the increase inbiochemical parameters of renal function–serum urea (up to 63.1%) and creatinine concentrations (upto 23.3%) were observed for both polyoxotungstates. In addition, the detected biochemical changeswere in accordance with kidney and liver histhopathological analysis. Accordingly, the hepatotoxic andnephrotoxic effects of these potential antidiabetic polyoxotungstates could be considered as mild.
T2  - RSC Advances
T1  - In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system
VL  - 10
IS  - 5
SP  - 2846
EP  - 2855
DO  - 10.1039/C9RA09790B
ER  - 
@article{
author = "Dinčić, Marko and Čolović, Mirjana B. and Sarić Matutinović, Marija and Ćetković, Mila and Kravić-Stevović, Tamara K. and Mougharbel, Ali S. and Todorović, Jasna and Ignjatović, Svetlana and Radosavljević, Branimir and Milisavljević, Milan and Kortz, Ulrich and Krstić, Danijela Z.",
year = "2020",
abstract = "In this study, thein vivohypoglycemic effect of a donut-shaped polyanion salt (NH4)14[Na@P5W30O110]$31H2O{NaP5W30} and its Ag-containing derivative K14[Ag@P5W30O110]$22H2O$6KCl {AgP5W30}, as wellas their hepatotoxicity and nephrotoxicity, was evaluated. In the screening hypoglycemic study,Wistaralbinorats with streptozotocin induced diabetes were treated intraperitoneally with three single doses (5,10, and 20 mg per kg per b.w.) of both investigated polyoxotungstates. The blood glucose levels,measured before and after 2, 4 and 6 h polyoxotungstate application, showed that both studiedcompounds induced the most pronounced and time dependent glucose lowering effects at the doses of20 mg kg1. Thus, daily doses of 20 mg kg1were administered toWistar albinorats orally for 14 days infurther toxicity examinations. The serum glucose concentration and biochemical parameters of kidneyand liver function, as well as a histopathological analysis of kidney and liver tissues were evaluated 14days after the polyoxotungstate administration. Both investigated compounds did not induce statisticallysignificant alterations of the serum glucose and uric acid concentrations, as well as some of the liverfunction markers (serum alanine and aspartate aminotransferases, and alkaline phosphatase activities).However, the significant decrease in serum total protein and albumin concentrations and the increase inbiochemical parameters of renal function–serum urea (up to 63.1%) and creatinine concentrations (upto 23.3%) were observed for both polyoxotungstates. In addition, the detected biochemical changeswere in accordance with kidney and liver histhopathological analysis. Accordingly, the hepatotoxic andnephrotoxic effects of these potential antidiabetic polyoxotungstates could be considered as mild.",
journal = "RSC Advances",
title = "In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system",
volume = "10",
number = "5",
pages = "2846-2855",
doi = "10.1039/C9RA09790B"
}
Dinčić, M., Čolović, M. B., Sarić Matutinović, M., Ćetković, M., Kravić-Stevović, T. K., Mougharbel, A. S., Todorović, J., Ignjatović, S., Radosavljević, B., Milisavljević, M., Kortz, U.,& Krstić, D. Z.. (2020). In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system. in RSC Advances, 10(5), 2846-2855.
https://doi.org/10.1039/C9RA09790B
Dinčić M, Čolović MB, Sarić Matutinović M, Ćetković M, Kravić-Stevović TK, Mougharbel AS, Todorović J, Ignjatović S, Radosavljević B, Milisavljević M, Kortz U, Krstić DZ. In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system. in RSC Advances. 2020;10(5):2846-2855.
doi:10.1039/C9RA09790B .
Dinčić, Marko, Čolović, Mirjana B., Sarić Matutinović, Marija, Ćetković, Mila, Kravić-Stevović, Tamara K., Mougharbel, Ali S., Todorović, Jasna, Ignjatović, Svetlana, Radosavljević, Branimir, Milisavljević, Milan, Kortz, Ulrich, Krstić, Danijela Z., "In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system" in RSC Advances, 10, no. 5 (2020):2846-2855,
https://doi.org/10.1039/C9RA09790B . .
2
2

Analytical techniques for multiplex analysis of protein biomarkers

Van Gool, Alain; Corrales, Fernado; Čolović, Mirjana B.; Krstić, Danijela; Oliver-Martos, Begona; Martínez-Cáceres, Eva; Jakasa, Ivone; Gajski, Goran; Brun, Virginie; Kyriacou, Kyriacos; Burzynska-Pedziwiatr, Izabela; Wozniak, Lucyna Alicja; Nierkens, Stephan; Pascual García, César; Katrlik, Jaroslav; Bojić-Trbojević, Žanka; Vacek, Jan; Llorente, Alicia; Antohe, Felicia; Suica, Viorel; Suarez, Guillaume; T’Kindt, Ruben; Martin, Petra; Penque, Deborah; Martins, Ines Lanca; Bodoki, Ede; Iacob, Bogdan-Cezar; Aydindogan, Eda; Timur, Suna; Allinson, John; Sutton, Christopher; Luider, Theo; Wittfooth, Saara; Sammar, Marei

(2020)

TY  - JOUR
AU  - Van Gool, Alain
AU  - Corrales, Fernado
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela
AU  - Oliver-Martos, Begona
AU  - Martínez-Cáceres, Eva
AU  - Jakasa, Ivone
AU  - Gajski, Goran
AU  - Brun, Virginie
AU  - Kyriacou, Kyriacos
AU  - Burzynska-Pedziwiatr, Izabela
AU  - Wozniak, Lucyna Alicja
AU  - Nierkens, Stephan
AU  - Pascual García, César
AU  - Katrlik, Jaroslav
AU  - Bojić-Trbojević, Žanka
AU  - Vacek, Jan
AU  - Llorente, Alicia
AU  - Antohe, Felicia
AU  - Suica, Viorel
AU  - Suarez, Guillaume
AU  - T’Kindt, Ruben
AU  - Martin, Petra
AU  - Penque, Deborah
AU  - Martins, Ines Lanca
AU  - Bodoki, Ede
AU  - Iacob, Bogdan-Cezar
AU  - Aydindogan, Eda
AU  - Timur, Suna
AU  - Allinson, John
AU  - Sutton, Christopher
AU  - Luider, Theo
AU  - Wittfooth, Saara
AU  - Sammar, Marei
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9018
AB  - Introduction: The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, capabilities to analyze multiple protein biomarkers in one assay are highly interesting for biomedical research. Areas covered: We here review multiple methods that are suitable for robust, high throughput, standardized, and affordable analysis of protein biomarkers in a multiplex format. We describe innovative developments in immunoassays, the vanguard of methods in clinical laboratories, and mass spectrometry, increasingly implemented for protein biomarker analysis. Moreover, emerging techniques are discussed with potentially improved protein capture, separation, and detection that will further boost multiplex analyses. Expert commentary: The development of clinically applied multiplex protein biomarker assays is essential as multi-protein signatures provide more comprehensive information about biological systems than single biomarkers, leading to improved insights in mechanisms of disease, diagnostics, and the effect of personalized medicine.
T2  - Expert Review of Proteomics
T1  - Analytical techniques for multiplex analysis of protein biomarkers
VL  - 17
IS  - 4
SP  - 257
EP  - 273
DO  - 10.1080/14789450.2020.1763174
ER  - 
@article{
author = "Van Gool, Alain and Corrales, Fernado and Čolović, Mirjana B. and Krstić, Danijela and Oliver-Martos, Begona and Martínez-Cáceres, Eva and Jakasa, Ivone and Gajski, Goran and Brun, Virginie and Kyriacou, Kyriacos and Burzynska-Pedziwiatr, Izabela and Wozniak, Lucyna Alicja and Nierkens, Stephan and Pascual García, César and Katrlik, Jaroslav and Bojić-Trbojević, Žanka and Vacek, Jan and Llorente, Alicia and Antohe, Felicia and Suica, Viorel and Suarez, Guillaume and T’Kindt, Ruben and Martin, Petra and Penque, Deborah and Martins, Ines Lanca and Bodoki, Ede and Iacob, Bogdan-Cezar and Aydindogan, Eda and Timur, Suna and Allinson, John and Sutton, Christopher and Luider, Theo and Wittfooth, Saara and Sammar, Marei",
year = "2020",
abstract = "Introduction: The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, capabilities to analyze multiple protein biomarkers in one assay are highly interesting for biomedical research. Areas covered: We here review multiple methods that are suitable for robust, high throughput, standardized, and affordable analysis of protein biomarkers in a multiplex format. We describe innovative developments in immunoassays, the vanguard of methods in clinical laboratories, and mass spectrometry, increasingly implemented for protein biomarker analysis. Moreover, emerging techniques are discussed with potentially improved protein capture, separation, and detection that will further boost multiplex analyses. Expert commentary: The development of clinically applied multiplex protein biomarker assays is essential as multi-protein signatures provide more comprehensive information about biological systems than single biomarkers, leading to improved insights in mechanisms of disease, diagnostics, and the effect of personalized medicine.",
journal = "Expert Review of Proteomics",
title = "Analytical techniques for multiplex analysis of protein biomarkers",
volume = "17",
number = "4",
pages = "257-273",
doi = "10.1080/14789450.2020.1763174"
}
Van Gool, A., Corrales, F., Čolović, M. B., Krstić, D., Oliver-Martos, B., Martínez-Cáceres, E., Jakasa, I., Gajski, G., Brun, V., Kyriacou, K., Burzynska-Pedziwiatr, I., Wozniak, L. A., Nierkens, S., Pascual García, C., Katrlik, J., Bojić-Trbojević, Ž., Vacek, J., Llorente, A., Antohe, F., Suica, V., Suarez, G., T’Kindt, R., Martin, P., Penque, D., Martins, I. L., Bodoki, E., Iacob, B., Aydindogan, E., Timur, S., Allinson, J., Sutton, C., Luider, T., Wittfooth, S.,& Sammar, M.. (2020). Analytical techniques for multiplex analysis of protein biomarkers. in Expert Review of Proteomics, 17(4), 257-273.
https://doi.org/10.1080/14789450.2020.1763174
Van Gool A, Corrales F, Čolović MB, Krstić D, Oliver-Martos B, Martínez-Cáceres E, Jakasa I, Gajski G, Brun V, Kyriacou K, Burzynska-Pedziwiatr I, Wozniak LA, Nierkens S, Pascual García C, Katrlik J, Bojić-Trbojević Ž, Vacek J, Llorente A, Antohe F, Suica V, Suarez G, T’Kindt R, Martin P, Penque D, Martins IL, Bodoki E, Iacob B, Aydindogan E, Timur S, Allinson J, Sutton C, Luider T, Wittfooth S, Sammar M. Analytical techniques for multiplex analysis of protein biomarkers. in Expert Review of Proteomics. 2020;17(4):257-273.
doi:10.1080/14789450.2020.1763174 .
Van Gool, Alain, Corrales, Fernado, Čolović, Mirjana B., Krstić, Danijela, Oliver-Martos, Begona, Martínez-Cáceres, Eva, Jakasa, Ivone, Gajski, Goran, Brun, Virginie, Kyriacou, Kyriacos, Burzynska-Pedziwiatr, Izabela, Wozniak, Lucyna Alicja, Nierkens, Stephan, Pascual García, César, Katrlik, Jaroslav, Bojić-Trbojević, Žanka, Vacek, Jan, Llorente, Alicia, Antohe, Felicia, Suica, Viorel, Suarez, Guillaume, T’Kindt, Ruben, Martin, Petra, Penque, Deborah, Martins, Ines Lanca, Bodoki, Ede, Iacob, Bogdan-Cezar, Aydindogan, Eda, Timur, Suna, Allinson, John, Sutton, Christopher, Luider, Theo, Wittfooth, Saara, Sammar, Marei, "Analytical techniques for multiplex analysis of protein biomarkers" in Expert Review of Proteomics, 17, no. 4 (2020):257-273,
https://doi.org/10.1080/14789450.2020.1763174 . .
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5
8

Lithium - Pharmacological and Toxicological Aspects: The Current State of the Art

Medić, Branislava; Stojanović, Marko; Stimec, Bojan V.; Divac, Nevena; Savić Vujović, Katarina; Stojanović, Radan; Čolović, Mirjana B.; Krstić, Danijela; Prostran, Milica

(2020)

TY  - JOUR
AU  - Medić, Branislava
AU  - Stojanović, Marko
AU  - Stimec, Bojan V.
AU  - Divac, Nevena
AU  - Savić Vujović, Katarina
AU  - Stojanović, Radan
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela
AU  - Prostran, Milica
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8838
AB  - Lithium is the smallest monovalent cation with many different biological effects. Although lithium is present in the pharmacotherapy of psychiatric illnesses for decades, its precise mechanism of action is still not clarified. Today lithium represents first-line therapy for bipolar disorders (because it possesses both antimanic and antidepressant properties) and the adjunctive treatment for major depression (due to its antisuicidal effects). Beside, lithium showed some protective effects in neurological diseases including acute neural injury, chronic degenerative conditions, Alzheimer's disease as well as in treating leucopenia, hepatitis and some renal diseases. Recent evidence suggested that lithium also possesses some anticancer properties due to its inhibition of Glycogen Synthase Kinase 3 beta (GSK3β) which is included in the regulation of a lot of important cellular processes such as: glycogen metabolism, inflammation, immunomodulation, apoptosis, tissue injury, regeneration etc. Although recent evidence suggested a potential utility of lithium in different conditions, its broader use in clinical practice still trails. The reason for this is a narrow therapeutic index of lithium, numerous toxic effects in various organ systems and some clinically relevant interactions with other drugs. Additionally, it is necessary to perform more preclinical as well as clinical studies in order to a precise therapeutic range of lithium, as well as its detailed mechanism of action. The aim of this review is to summarize the current knowledge concerning the pharmacological and toxicological effects of lithium. © 2020 Bentham Science Publishers.
T2  - Current Medicinal Chemistry
T1  - Lithium - Pharmacological and Toxicological Aspects: The Current State of the Art
VL  - 27
IS  - 3
SP  - 337
EP  - 351
DO  - 10.2174/0929867325666180904124733
ER  - 
@article{
author = "Medić, Branislava and Stojanović, Marko and Stimec, Bojan V. and Divac, Nevena and Savić Vujović, Katarina and Stojanović, Radan and Čolović, Mirjana B. and Krstić, Danijela and Prostran, Milica",
year = "2020",
abstract = "Lithium is the smallest monovalent cation with many different biological effects. Although lithium is present in the pharmacotherapy of psychiatric illnesses for decades, its precise mechanism of action is still not clarified. Today lithium represents first-line therapy for bipolar disorders (because it possesses both antimanic and antidepressant properties) and the adjunctive treatment for major depression (due to its antisuicidal effects). Beside, lithium showed some protective effects in neurological diseases including acute neural injury, chronic degenerative conditions, Alzheimer's disease as well as in treating leucopenia, hepatitis and some renal diseases. Recent evidence suggested that lithium also possesses some anticancer properties due to its inhibition of Glycogen Synthase Kinase 3 beta (GSK3β) which is included in the regulation of a lot of important cellular processes such as: glycogen metabolism, inflammation, immunomodulation, apoptosis, tissue injury, regeneration etc. Although recent evidence suggested a potential utility of lithium in different conditions, its broader use in clinical practice still trails. The reason for this is a narrow therapeutic index of lithium, numerous toxic effects in various organ systems and some clinically relevant interactions with other drugs. Additionally, it is necessary to perform more preclinical as well as clinical studies in order to a precise therapeutic range of lithium, as well as its detailed mechanism of action. The aim of this review is to summarize the current knowledge concerning the pharmacological and toxicological effects of lithium. © 2020 Bentham Science Publishers.",
journal = "Current Medicinal Chemistry",
title = "Lithium - Pharmacological and Toxicological Aspects: The Current State of the Art",
volume = "27",
number = "3",
pages = "337-351",
doi = "10.2174/0929867325666180904124733"
}
Medić, B., Stojanović, M., Stimec, B. V., Divac, N., Savić Vujović, K., Stojanović, R., Čolović, M. B., Krstić, D.,& Prostran, M.. (2020). Lithium - Pharmacological and Toxicological Aspects: The Current State of the Art. in Current Medicinal Chemistry, 27(3), 337-351.
https://doi.org/10.2174/0929867325666180904124733
Medić B, Stojanović M, Stimec BV, Divac N, Savić Vujović K, Stojanović R, Čolović MB, Krstić D, Prostran M. Lithium - Pharmacological and Toxicological Aspects: The Current State of the Art. in Current Medicinal Chemistry. 2020;27(3):337-351.
doi:10.2174/0929867325666180904124733 .
Medić, Branislava, Stojanović, Marko, Stimec, Bojan V., Divac, Nevena, Savić Vujović, Katarina, Stojanović, Radan, Čolović, Mirjana B., Krstić, Danijela, Prostran, Milica, "Lithium - Pharmacological and Toxicological Aspects: The Current State of the Art" in Current Medicinal Chemistry, 27, no. 3 (2020):337-351,
https://doi.org/10.2174/0929867325666180904124733 . .
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11

Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays

Bošnjaković-Pavlović, Nada; Xu, Xiao; Krstić, Danijela Z.; Gillet, Jean-Michel; Wei, Yongge; Wu, Pingfan; Čolović, Mirjana B.; Spasojević-de Biré, Anne

(2019)

TY  - JOUR
AU  - Bošnjaković-Pavlović, Nada
AU  - Xu, Xiao
AU  - Krstić, Danijela Z.
AU  - Gillet, Jean-Michel
AU  - Wei, Yongge
AU  - Wu, Pingfan
AU  - Čolović, Mirjana B.
AU  - Spasojević-de Biré, Anne
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0162013418306998
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8355
AB  - The influence of three functionalized hexavanadates (V6): Na2 [V6O13{(OCH2)3CCH3}2], [H2]2 [V6O13{(OCH2)3CCH2OCOCH2CH3}2] and [(C4H9)4N]2 [V6O13{(OCH2)3CCH2OOC(CH3)2-COOH}2 on Na+/K+-ATPase activity, was investigated in vitro. Including compounds already tested by Xu et al. (Journal of Inorganic Biochemistry 161 (2016) 27–36), all functionalized hexavanadates inhibit the activity of Na+/K+-ATPase in a dose-dependent manner but with different inhibitory potencies. Na2 [V6O13{(OCH2)3CCH3}2] was found to have the best inhibition properties - showing 50% inhibition IC50 = 5.50 × 10−5 M, while [(C4H9)4N]2 [V6O13{(OCH2)3CCH2OOC(CH3)2-COOH}2] showed the lowest inhibitory power, IC50 = 1.31 × 10−4 M. In order to understand the bioactivity of functionalized hexavanadates series, we have also used a combined theoretical approach: determination of electrostatic potential from ab initio theoretical calculations and computation of the molecular interaction field (MIF) surface. © 2019
T2  - Journal of Inorganic Biochemistry
T1  - Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays
VL  - 198
SP  - 110720
DO  - 10.1016/j.jinorgbio.2019.110720
ER  - 
@article{
author = "Bošnjaković-Pavlović, Nada and Xu, Xiao and Krstić, Danijela Z. and Gillet, Jean-Michel and Wei, Yongge and Wu, Pingfan and Čolović, Mirjana B. and Spasojević-de Biré, Anne",
year = "2019",
abstract = "The influence of three functionalized hexavanadates (V6): Na2 [V6O13{(OCH2)3CCH3}2], [H2]2 [V6O13{(OCH2)3CCH2OCOCH2CH3}2] and [(C4H9)4N]2 [V6O13{(OCH2)3CCH2OOC(CH3)2-COOH}2 on Na+/K+-ATPase activity, was investigated in vitro. Including compounds already tested by Xu et al. (Journal of Inorganic Biochemistry 161 (2016) 27–36), all functionalized hexavanadates inhibit the activity of Na+/K+-ATPase in a dose-dependent manner but with different inhibitory potencies. Na2 [V6O13{(OCH2)3CCH3}2] was found to have the best inhibition properties - showing 50% inhibition IC50 = 5.50 × 10−5 M, while [(C4H9)4N]2 [V6O13{(OCH2)3CCH2OOC(CH3)2-COOH}2] showed the lowest inhibitory power, IC50 = 1.31 × 10−4 M. In order to understand the bioactivity of functionalized hexavanadates series, we have also used a combined theoretical approach: determination of electrostatic potential from ab initio theoretical calculations and computation of the molecular interaction field (MIF) surface. © 2019",
journal = "Journal of Inorganic Biochemistry",
title = "Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays",
volume = "198",
pages = "110720",
doi = "10.1016/j.jinorgbio.2019.110720"
}
Bošnjaković-Pavlović, N., Xu, X., Krstić, D. Z., Gillet, J., Wei, Y., Wu, P., Čolović, M. B.,& Spasojević-de Biré, A.. (2019). Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays. in Journal of Inorganic Biochemistry, 198, 110720.
https://doi.org/10.1016/j.jinorgbio.2019.110720
Bošnjaković-Pavlović N, Xu X, Krstić DZ, Gillet J, Wei Y, Wu P, Čolović MB, Spasojević-de Biré A. Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays. in Journal of Inorganic Biochemistry. 2019;198:110720.
doi:10.1016/j.jinorgbio.2019.110720 .
Bošnjaković-Pavlović, Nada, Xu, Xiao, Krstić, Danijela Z., Gillet, Jean-Michel, Wei, Yongge, Wu, Pingfan, Čolović, Mirjana B., Spasojević-de Biré, Anne, "Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays" in Journal of Inorganic Biochemistry, 198 (2019):110720,
https://doi.org/10.1016/j.jinorgbio.2019.110720 . .
4
3
3

Tetravalent Metal Ion Guests in Polyoxopalladate Chemistry: Synthesis and Anticancer Activity of [MO 8 Pd 12 (PO 4 ) 8 ] 12– (M = Sn IV , Pb IV )

Yang, Peng; Ma, Tian; Lang, Zhongling; Misirlić-Denčić, Sonja; Isaković, Anđelka; Benyei, Attila; Čolović, Mirjana B.; Marković, Ivanka; Krstić, Danijela Z.; Poblet, Josep M.; Lin, Zhengguo; Kortz, Ulrich

(2019)

TY  - JOUR
AU  - Yang, Peng
AU  - Ma, Tian
AU  - Lang, Zhongling
AU  - Misirlić-Denčić, Sonja
AU  - Isaković, Anđelka
AU  - Benyei, Attila
AU  - Čolović, Mirjana B.
AU  - Marković, Ivanka
AU  - Krstić, Danijela Z.
AU  - Poblet, Josep M.
AU  - Lin, Zhengguo
AU  - Kortz, Ulrich
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8499
AB  - The first two examples of polyoxopalladates(II) (POPs) containing tetravalent metal ion guests, [MO8Pd12(PO4)8]12- (M = SnIV, PbIV), have been prepared and structurally characterized in the solid state, solution, and gas phase. The interactions of the metal ion guests and the palladium-oxo shell were studied by theoretical calculations. The POPs were shown to possess anticancer activity by causing oxidative stress inducing caspase activation and consecutive apoptosis of leukemic cells.
T2  - Inorganic Chemistry
T1  - Tetravalent Metal Ion Guests in Polyoxopalladate Chemistry: Synthesis and Anticancer Activity of [MO 8 Pd 12 (PO 4 ) 8 ] 12– (M = Sn IV , Pb IV )
VL  - 58
IS  - 17
SP  - 11294
EP  - 11299
DO  - 10.1021/acs.inorgchem.9b01129
ER  - 
@article{
author = "Yang, Peng and Ma, Tian and Lang, Zhongling and Misirlić-Denčić, Sonja and Isaković, Anđelka and Benyei, Attila and Čolović, Mirjana B. and Marković, Ivanka and Krstić, Danijela Z. and Poblet, Josep M. and Lin, Zhengguo and Kortz, Ulrich",
year = "2019",
abstract = "The first two examples of polyoxopalladates(II) (POPs) containing tetravalent metal ion guests, [MO8Pd12(PO4)8]12- (M = SnIV, PbIV), have been prepared and structurally characterized in the solid state, solution, and gas phase. The interactions of the metal ion guests and the palladium-oxo shell were studied by theoretical calculations. The POPs were shown to possess anticancer activity by causing oxidative stress inducing caspase activation and consecutive apoptosis of leukemic cells.",
journal = "Inorganic Chemistry",
title = "Tetravalent Metal Ion Guests in Polyoxopalladate Chemistry: Synthesis and Anticancer Activity of [MO 8 Pd 12 (PO 4 ) 8 ] 12– (M = Sn IV , Pb IV )",
volume = "58",
number = "17",
pages = "11294-11299",
doi = "10.1021/acs.inorgchem.9b01129"
}
Yang, P., Ma, T., Lang, Z., Misirlić-Denčić, S., Isaković, A., Benyei, A., Čolović, M. B., Marković, I., Krstić, D. Z., Poblet, J. M., Lin, Z.,& Kortz, U.. (2019). Tetravalent Metal Ion Guests in Polyoxopalladate Chemistry: Synthesis and Anticancer Activity of [MO 8 Pd 12 (PO 4 ) 8 ] 12– (M = Sn IV , Pb IV ). in Inorganic Chemistry, 58(17), 11294-11299.
https://doi.org/10.1021/acs.inorgchem.9b01129
Yang P, Ma T, Lang Z, Misirlić-Denčić S, Isaković A, Benyei A, Čolović MB, Marković I, Krstić DZ, Poblet JM, Lin Z, Kortz U. Tetravalent Metal Ion Guests in Polyoxopalladate Chemistry: Synthesis and Anticancer Activity of [MO 8 Pd 12 (PO 4 ) 8 ] 12– (M = Sn IV , Pb IV ). in Inorganic Chemistry. 2019;58(17):11294-11299.
doi:10.1021/acs.inorgchem.9b01129 .
Yang, Peng, Ma, Tian, Lang, Zhongling, Misirlić-Denčić, Sonja, Isaković, Anđelka, Benyei, Attila, Čolović, Mirjana B., Marković, Ivanka, Krstić, Danijela Z., Poblet, Josep M., Lin, Zhengguo, Kortz, Ulrich, "Tetravalent Metal Ion Guests in Polyoxopalladate Chemistry: Synthesis and Anticancer Activity of [MO 8 Pd 12 (PO 4 ) 8 ] 12– (M = Sn IV , Pb IV )" in Inorganic Chemistry, 58, no. 17 (2019):11294-11299,
https://doi.org/10.1021/acs.inorgchem.9b01129 . .
8
6
6

Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue

Stojanović, Marija; Todorović, Dajana D.; Šćepanović, Ljiljana; Mitrović, Dušan M.; Borozan, Sunčica Z.; Dragutinović, Vesna V.; Labudović-Borović, Milica; Krstić, Danijela Z.; Čolović, Mirjana B.; Đurić, Dragan M.

(2018)

TY  - JOUR
AU  - Stojanović, Marija
AU  - Todorović, Dajana D.
AU  - Šćepanović, Ljiljana
AU  - Mitrović, Dušan M.
AU  - Borozan, Sunčica Z.
AU  - Dragutinović, Vesna V.
AU  - Labudović-Borović, Milica
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - http://link.springer.com/10.1007/s11010-018-3311-2
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8060
AB  - The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that Cys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure.
T2  - Molecular and Cellular Biochemistry
T1  - Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue
VL  - 448
IS  - 1-2
SP  - 43
EP  - 50
DO  - 10.1007/s11010-018-3311-2
ER  - 
@article{
author = "Stojanović, Marija and Todorović, Dajana D. and Šćepanović, Ljiljana and Mitrović, Dušan M. and Borozan, Sunčica Z. and Dragutinović, Vesna V. and Labudović-Borović, Milica and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Dragan M.",
year = "2018",
abstract = "The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that Cys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure.",
journal = "Molecular and Cellular Biochemistry",
title = "Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue",
volume = "448",
number = "1-2",
pages = "43-50",
doi = "10.1007/s11010-018-3311-2"
}
Stojanović, M., Todorović, D. D., Šćepanović, L., Mitrović, D. M., Borozan, S. Z., Dragutinović, V. V., Labudović-Borović, M., Krstić, D. Z., Čolović, M. B.,& Đurić, D. M.. (2018). Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue. in Molecular and Cellular Biochemistry, 448(1-2), 43-50.
https://doi.org/10.1007/s11010-018-3311-2
Stojanović M, Todorović DD, Šćepanović L, Mitrović DM, Borozan SZ, Dragutinović VV, Labudović-Borović M, Krstić DZ, Čolović MB, Đurić DM. Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue. in Molecular and Cellular Biochemistry. 2018;448(1-2):43-50.
doi:10.1007/s11010-018-3311-2 .
Stojanović, Marija, Todorović, Dajana D., Šćepanović, Ljiljana, Mitrović, Dušan M., Borozan, Sunčica Z., Dragutinović, Vesna V., Labudović-Borović, Milica, Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Dragan M., "Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue" in Molecular and Cellular Biochemistry, 448, no. 1-2 (2018):43-50,
https://doi.org/10.1007/s11010-018-3311-2 . .
8
8
9

Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals

Čolović, Mirjana B.; Vasić, Vesna M.; Đurić, Dragan M.; Krstić, Danijela Z.

(2018)

TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Vasić, Vesna M.
AU  - Đurić, Dragan M.
AU  - Krstić, Danijela Z.
PY  - 2018
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1926
AB  - Background: Sulphur is an abundant element in biological systems, which plays an important role in processes essential for life as a constituent of proteins, vitamins and other crucial biomolecules. The major source of sulphur for humans is plants being able to use inorganic sulphur in the purpose of sulphur-containing amino acids synthesis. Sulphur-containing amino acids include methionine, cysteine, homocysteine, and taurine. Methionine and cysteine are classified as proteinogenic, canonic amino acids incorporated in protein structure. Sulphur amino acids are involved in the synthesis of intracellular antioxidants such as glutathione and N-acetyl cysteine. Moreover, naturally occurring sulphur-containing ligands are effective and safe detoxifying agents, often used in order to prevent toxic metal ions effects and their accumulation in human body. Methods: Literature search for peer-reviewed articles was performed using PubMed and Scopus databases, and utilizing appropriate keywords. Results: This review is focused on sulphur-containing amino acids - methionine, cysteine, taurine, and their derivatives - glutathione and N-acetylcysteine, and their defense effects as antioxidant agents against free radicals. Additionally, the protective effects of sulphur-containing ligands against the toxic effects of heavy and transition metal ions, and their reactivation role towards the enzyme inhibition are described. Conclusion: Sulphur-containing amino acids represent a powerful part of cell antioxidant system. Thus, they are essential in the maintenance of normal cellular functions and health. In addition to their worthy antioxidant action, sulphur-containing amino acids may offer a chelating site for heavy metals. Accordingly, they may be supplemented during chelating therapy, providing beneficial effects in eliminating toxic metals.
T2  - Current Medicinal Chemistry
T1  - Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals
VL  - 25
IS  - 3
SP  - 324
EP  - 335
DO  - 10.2174/0929867324666170609075434
ER  - 
@article{
author = "Čolović, Mirjana B. and Vasić, Vesna M. and Đurić, Dragan M. and Krstić, Danijela Z.",
year = "2018",
abstract = "Background: Sulphur is an abundant element in biological systems, which plays an important role in processes essential for life as a constituent of proteins, vitamins and other crucial biomolecules. The major source of sulphur for humans is plants being able to use inorganic sulphur in the purpose of sulphur-containing amino acids synthesis. Sulphur-containing amino acids include methionine, cysteine, homocysteine, and taurine. Methionine and cysteine are classified as proteinogenic, canonic amino acids incorporated in protein structure. Sulphur amino acids are involved in the synthesis of intracellular antioxidants such as glutathione and N-acetyl cysteine. Moreover, naturally occurring sulphur-containing ligands are effective and safe detoxifying agents, often used in order to prevent toxic metal ions effects and their accumulation in human body. Methods: Literature search for peer-reviewed articles was performed using PubMed and Scopus databases, and utilizing appropriate keywords. Results: This review is focused on sulphur-containing amino acids - methionine, cysteine, taurine, and their derivatives - glutathione and N-acetylcysteine, and their defense effects as antioxidant agents against free radicals. Additionally, the protective effects of sulphur-containing ligands against the toxic effects of heavy and transition metal ions, and their reactivation role towards the enzyme inhibition are described. Conclusion: Sulphur-containing amino acids represent a powerful part of cell antioxidant system. Thus, they are essential in the maintenance of normal cellular functions and health. In addition to their worthy antioxidant action, sulphur-containing amino acids may offer a chelating site for heavy metals. Accordingly, they may be supplemented during chelating therapy, providing beneficial effects in eliminating toxic metals.",
journal = "Current Medicinal Chemistry",
title = "Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals",
volume = "25",
number = "3",
pages = "324-335",
doi = "10.2174/0929867324666170609075434"
}
Čolović, M. B., Vasić, V. M., Đurić, D. M.,& Krstić, D. Z.. (2018). Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals. in Current Medicinal Chemistry, 25(3), 324-335.
https://doi.org/10.2174/0929867324666170609075434
Čolović MB, Vasić VM, Đurić DM, Krstić DZ. Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals. in Current Medicinal Chemistry. 2018;25(3):324-335.
doi:10.2174/0929867324666170609075434 .
Čolović, Mirjana B., Vasić, Vesna M., Đurić, Dragan M., Krstić, Danijela Z., "Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals" in Current Medicinal Chemistry, 25, no. 3 (2018):324-335,
https://doi.org/10.2174/0929867324666170609075434 . .
39
30
35

Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field

Dinčić, Marko; Krstić, Danijela Z.; Čolović, Mirjana B.; Nešović Ostojić, Jelena; Kovačević, Sanjin; De Luka, Silvio R.; Đorđević, Drago M.; Ćirković, Saša; Brkić, Predrag; Todorović, Jasna

(2018)

TY  - JOUR
AU  - Dinčić, Marko
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Nešović Ostojić, Jelena
AU  - Kovačević, Sanjin
AU  - De Luka, Silvio R.
AU  - Đorđević, Drago M.
AU  - Ćirković, Saša
AU  - Brkić, Predrag
AU  - Todorović, Jasna
PY  - 2018
UR  - https://www.tandfonline.com/doi/full/10.1080/09553002.2018.1518611
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7957
AB  - Purpose: It is considered that exposure to static magnetic fields (SMF) may have both detrimental and therapeutic effect, but the mechanism of SMF influence on the living organisms is not well understood. Since the adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) are involved in both physiological and pathological processes, the modulation of Na+/K+-ATPase, ecto-ATPases and AChE activities, as well as oxidative stress responses were followed in synaptosomes isolated from rats after chronic exposure toward differently oriented SMF. Material and methods: Wistar albino rats were randomly divided into three experimental groups (six animals per group): Up and Down group - exposed to upward and downward oriented SMF, respectively, and Control group. After 50 days, the rats were sacrificed, and synaptosomes were isolated from the whole rat brain and used for testing the enzyme activities and oxidative stress parameters. Results: Chronic exposure to 1 mT SMF significantly increased ATPases, AChE activities, and malondialdehyde (MDA) level in both exposed groups, compared to control values. The significant decrease in synaptosomal catalase activity (1.48 ± 0.17 U/mg protein) induced by exposure to the downward oriented field, compared to those obtained for Control group (2.60 ± 0.29 U/mg protein), and Up group (2.72 ± 0.21 U/mg protein). Conclusions: It could be concluded that chronic exposure to differently oriented SMF increases ATPases and AChE activities in rat synaptosomes. Since brain ATPases and AChE have important roles in the pathogenesis of several neurological diseases, SMF influence on the activity of these enzymes may have potential therapeutic importance. © 2018, Copyright © 2018 Taylor & Francis Group, LLC.
T2  - International Journal of Radiation Biology
T1  - Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field
VL  - 94
IS  - 11
SP  - 1062
EP  - 1071
DO  - 10.1080/09553002.2018.1518611
ER  - 
@article{
author = "Dinčić, Marko and Krstić, Danijela Z. and Čolović, Mirjana B. and Nešović Ostojić, Jelena and Kovačević, Sanjin and De Luka, Silvio R. and Đorđević, Drago M. and Ćirković, Saša and Brkić, Predrag and Todorović, Jasna",
year = "2018",
abstract = "Purpose: It is considered that exposure to static magnetic fields (SMF) may have both detrimental and therapeutic effect, but the mechanism of SMF influence on the living organisms is not well understood. Since the adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) are involved in both physiological and pathological processes, the modulation of Na+/K+-ATPase, ecto-ATPases and AChE activities, as well as oxidative stress responses were followed in synaptosomes isolated from rats after chronic exposure toward differently oriented SMF. Material and methods: Wistar albino rats were randomly divided into three experimental groups (six animals per group): Up and Down group - exposed to upward and downward oriented SMF, respectively, and Control group. After 50 days, the rats were sacrificed, and synaptosomes were isolated from the whole rat brain and used for testing the enzyme activities and oxidative stress parameters. Results: Chronic exposure to 1 mT SMF significantly increased ATPases, AChE activities, and malondialdehyde (MDA) level in both exposed groups, compared to control values. The significant decrease in synaptosomal catalase activity (1.48 ± 0.17 U/mg protein) induced by exposure to the downward oriented field, compared to those obtained for Control group (2.60 ± 0.29 U/mg protein), and Up group (2.72 ± 0.21 U/mg protein). Conclusions: It could be concluded that chronic exposure to differently oriented SMF increases ATPases and AChE activities in rat synaptosomes. Since brain ATPases and AChE have important roles in the pathogenesis of several neurological diseases, SMF influence on the activity of these enzymes may have potential therapeutic importance. © 2018, Copyright © 2018 Taylor & Francis Group, LLC.",
journal = "International Journal of Radiation Biology",
title = "Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field",
volume = "94",
number = "11",
pages = "1062-1071",
doi = "10.1080/09553002.2018.1518611"
}
Dinčić, M., Krstić, D. Z., Čolović, M. B., Nešović Ostojić, J., Kovačević, S., De Luka, S. R., Đorđević, D. M., Ćirković, S., Brkić, P.,& Todorović, J.. (2018). Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field. in International Journal of Radiation Biology, 94(11), 1062-1071.
https://doi.org/10.1080/09553002.2018.1518611
Dinčić M, Krstić DZ, Čolović MB, Nešović Ostojić J, Kovačević S, De Luka SR, Đorđević DM, Ćirković S, Brkić P, Todorović J. Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field. in International Journal of Radiation Biology. 2018;94(11):1062-1071.
doi:10.1080/09553002.2018.1518611 .
Dinčić, Marko, Krstić, Danijela Z., Čolović, Mirjana B., Nešović Ostojić, Jelena, Kovačević, Sanjin, De Luka, Silvio R., Đorđević, Drago M., Ćirković, Saša, Brkić, Predrag, Todorović, Jasna, "Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field" in International Journal of Radiation Biology, 94, no. 11 (2018):1062-1071,
https://doi.org/10.1080/09553002.2018.1518611 . .
1
3
2
1

The effects of N-acetyl-L-cysteine on subchronic methionine load in male wistar rats: Focus on standard biochemical parameters and markers of homocysteine metabolism in blood

Đurić, Aleksandar; Čolović, Mirjana B.; Krstić, Danijela Z.; Obrenović, Radmila; Micovic, Z; Đurić, Marija; Đurić, Dragan M.

(2018)

TY  - CONF
AU  - Đurić, Aleksandar
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Obrenović, Radmila
AU  - Micovic, Z
AU  - Đurić, Marija
AU  - Đurić, Dragan M.
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0021915018309547
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7954
C3  - Atherosclerosis
T1  - The effects of N-acetyl-L-cysteine on subchronic methionine load in male wistar rats: Focus on standard biochemical parameters and markers of homocysteine metabolism in blood
VL  - 275
SP  - e206
EP  - e207
DO  - 10.1016/j.atherosclerosis.2018.06.642
ER  - 
@conference{
author = "Đurić, Aleksandar and Čolović, Mirjana B. and Krstić, Danijela Z. and Obrenović, Radmila and Micovic, Z and Đurić, Marija and Đurić, Dragan M.",
year = "2018",
journal = "Atherosclerosis",
title = "The effects of N-acetyl-L-cysteine on subchronic methionine load in male wistar rats: Focus on standard biochemical parameters and markers of homocysteine metabolism in blood",
volume = "275",
pages = "e206-e207",
doi = "10.1016/j.atherosclerosis.2018.06.642"
}
Đurić, A., Čolović, M. B., Krstić, D. Z., Obrenović, R., Micovic, Z., Đurić, M.,& Đurić, D. M.. (2018). The effects of N-acetyl-L-cysteine on subchronic methionine load in male wistar rats: Focus on standard biochemical parameters and markers of homocysteine metabolism in blood. in Atherosclerosis, 275, e206-e207.
https://doi.org/10.1016/j.atherosclerosis.2018.06.642
Đurić A, Čolović MB, Krstić DZ, Obrenović R, Micovic Z, Đurić M, Đurić DM. The effects of N-acetyl-L-cysteine on subchronic methionine load in male wistar rats: Focus on standard biochemical parameters and markers of homocysteine metabolism in blood. in Atherosclerosis. 2018;275:e206-e207.
doi:10.1016/j.atherosclerosis.2018.06.642 .
Đurić, Aleksandar, Čolović, Mirjana B., Krstić, Danijela Z., Obrenović, Radmila, Micovic, Z, Đurić, Marija, Đurić, Dragan M., "The effects of N-acetyl-L-cysteine on subchronic methionine load in male wistar rats: Focus on standard biochemical parameters and markers of homocysteine metabolism in blood" in Atherosclerosis, 275 (2018):e206-e207,
https://doi.org/10.1016/j.atherosclerosis.2018.06.642 . .

Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters

Jakovljević-Uzelac, Jovana; Stanić, Marina; Krstić, Danijela Z.; Čolović, Mirjana B.; Đurić, Dragan M.

(2018)

TY  - JOUR
AU  - Jakovljević-Uzelac, Jovana
AU  - Stanić, Marina
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - http://link.springer.com/10.1007/s11010-017-3238-z
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7788
AB  - The objective of this study was to investigate in vitro effects of 10 A mu M dl-homocysteine (dl-Hcy), dl-homocysteine thiolactone-hydrochloride (dl-Hcy TLHC), and l-homocysteine thiolactone-hydrochloride (l-Hcy TLHC) on the oxygen consumption of rat heart tissue homogenate, as well as the involvement of the gasotransmitters NO, H2S and CO in the effects of the most toxic homocysteine compound, dl-Hcy TLHC. The possible contribution of the gasotransmitters in these effects was estimated by using the appropriate inhibitors of their synthesis (N (omega)-nitro-l-arginine methyl ester (l-NAME), dl-propargylglycine (dl-PAG), and zinc protoporphyrin IX (ZnPPR IX), respectively). The oxygen consumption of rat heart tissue homogenate was measured by Clark/type oxygen electrode in the absence and presence of the investigated compounds. All three homocysteine-based compounds caused a similar decrease in the oxygen consumption rate compared to control: 15.19 +/- 4.01%, 12.42 +/- 1.01%, and 16.43 +/- 4.52% for dl-Hcy, dl-Hcy TLHC, or l-Hcy TLHC, respectively. All applied inhibitors of gasotransmitter synthesis also decreased the oxygen consumption rate of tissue homogenate related to control: 13.53 +/- 1.35% for l-NAME (30 A mu M), 5.32 +/- 1.23% for dl-PAG (10 A mu M), and 5.56 +/- 1.39% for ZnPPR IX (10 A mu M). Simultaneous effect of l-NAME (30 A mu M) or ZnPPR IX (10 A mu M) with dl-Hcy TLHC (10 A mu M) caused a larger decrease of oxygen consumption compared to each of the substances individually. However, when dl-PAG (10 A mu M) was applied together with dl-Hcy TLHC (10 A mu M), it attenuated the effect of dl-Hcy TLHC from 12.42 +/- 1.01 to 9.22 +/- 1.58%. In conclusion, cardiotoxicity induced by Hcy-related compounds, which was shown in our previous research, could result from the inhibition of the oxygen consumption, and might be mediated by the certain gasotransmitters.
T2  - Molecular and Cellular Biochemistry
T1  - Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters
VL  - 444
IS  - 1-2
SP  - 143
EP  - 148
DO  - 10.1007/s11010-017-3238-z
ER  - 
@article{
author = "Jakovljević-Uzelac, Jovana and Stanić, Marina and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Dragan M.",
year = "2018",
abstract = "The objective of this study was to investigate in vitro effects of 10 A mu M dl-homocysteine (dl-Hcy), dl-homocysteine thiolactone-hydrochloride (dl-Hcy TLHC), and l-homocysteine thiolactone-hydrochloride (l-Hcy TLHC) on the oxygen consumption of rat heart tissue homogenate, as well as the involvement of the gasotransmitters NO, H2S and CO in the effects of the most toxic homocysteine compound, dl-Hcy TLHC. The possible contribution of the gasotransmitters in these effects was estimated by using the appropriate inhibitors of their synthesis (N (omega)-nitro-l-arginine methyl ester (l-NAME), dl-propargylglycine (dl-PAG), and zinc protoporphyrin IX (ZnPPR IX), respectively). The oxygen consumption of rat heart tissue homogenate was measured by Clark/type oxygen electrode in the absence and presence of the investigated compounds. All three homocysteine-based compounds caused a similar decrease in the oxygen consumption rate compared to control: 15.19 +/- 4.01%, 12.42 +/- 1.01%, and 16.43 +/- 4.52% for dl-Hcy, dl-Hcy TLHC, or l-Hcy TLHC, respectively. All applied inhibitors of gasotransmitter synthesis also decreased the oxygen consumption rate of tissue homogenate related to control: 13.53 +/- 1.35% for l-NAME (30 A mu M), 5.32 +/- 1.23% for dl-PAG (10 A mu M), and 5.56 +/- 1.39% for ZnPPR IX (10 A mu M). Simultaneous effect of l-NAME (30 A mu M) or ZnPPR IX (10 A mu M) with dl-Hcy TLHC (10 A mu M) caused a larger decrease of oxygen consumption compared to each of the substances individually. However, when dl-PAG (10 A mu M) was applied together with dl-Hcy TLHC (10 A mu M), it attenuated the effect of dl-Hcy TLHC from 12.42 +/- 1.01 to 9.22 +/- 1.58%. In conclusion, cardiotoxicity induced by Hcy-related compounds, which was shown in our previous research, could result from the inhibition of the oxygen consumption, and might be mediated by the certain gasotransmitters.",
journal = "Molecular and Cellular Biochemistry",
title = "Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters",
volume = "444",
number = "1-2",
pages = "143-148",
doi = "10.1007/s11010-017-3238-z"
}
Jakovljević-Uzelac, J., Stanić, M., Krstić, D. Z., Čolović, M. B.,& Đurić, D. M.. (2018). Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters. in Molecular and Cellular Biochemistry, 444(1-2), 143-148.
https://doi.org/10.1007/s11010-017-3238-z
Jakovljević-Uzelac J, Stanić M, Krstić DZ, Čolović MB, Đurić DM. Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters. in Molecular and Cellular Biochemistry. 2018;444(1-2):143-148.
doi:10.1007/s11010-017-3238-z .
Jakovljević-Uzelac, Jovana, Stanić, Marina, Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Dragan M., "Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters" in Molecular and Cellular Biochemistry, 444, no. 1-2 (2018):143-148,
https://doi.org/10.1007/s11010-017-3238-z . .
6
3
3

The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat

Kornjača, Duško; Živković, Vladimir I.; Krstić, Danijela Z.; Čolović, Mirjana B.; Đurić, Marko; Stanković, Sanja; Mutavdžin, Slavica; Jakovljević, Vladimir Lj.; Đurić, Dragan M.

(2018)

TY  - JOUR
AU  - Kornjača, Duško
AU  - Živković, Vladimir I.
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Đurić, Marko
AU  - Stanković, Sanja
AU  - Mutavdžin, Slavica
AU  - Jakovljević, Vladimir Lj.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641700041K
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7714
AB  - The aim of this study was to assess the effects of DL-homocysteine (DL-Hcy) and DL-homocysteine thiolactone (DL-Hcy TLHC) on selected serum biochemical parameters, markers of oxidative stress and the activities of antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD)) in the plasma, as well as on acetylcholinesterase (AChE) activity in the cardiac tissue homogenate in the rat. Male Wistar rats were divided into three groups as follows: control group (1 mL 0.9% NaCl, intraperitoneal (i.p.) injection), DL-Hcy group (8 mmol/kg body mass (b.m.), i.p.) or DL-Hcy TLHC group (8 mmol/kg b.m., i.p.). One hour after administration, the rats were euthanized, whole blood was collected for biochemical analysis, and the heart was excised. Following the i.p. administration of DL-Hcy and DL-Hcy TLHC, the activities of antioxidant enzymes were mostly significantly increased, while plasma malondialdehyde (MDA) was decreased. Administration of DL-Hcy and DL-Hcy TLHC significantly inhibited AChE activity in rat cardiac tissue. Our findings suggest that DL-Hcy and DL-Hcy TLHC exerted prooxidant effects; however, the decrease in MDA points to an inverse response to the increase in antioxidant enzyme activities. While both substances inhibited AChE activity in rat cardiac tissue, DL-Hcy TLHC induced stronger effects than DL-Hcy.
T2  - Archives of Biological Sciences
T1  - The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat
VL  - 70
IS  - 2
SP  - 241
EP  - 248
DO  - 10.2298/ABS170731041K
ER  - 
@article{
author = "Kornjača, Duško and Živković, Vladimir I. and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Marko and Stanković, Sanja and Mutavdžin, Slavica and Jakovljević, Vladimir Lj. and Đurić, Dragan M.",
year = "2018",
abstract = "The aim of this study was to assess the effects of DL-homocysteine (DL-Hcy) and DL-homocysteine thiolactone (DL-Hcy TLHC) on selected serum biochemical parameters, markers of oxidative stress and the activities of antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD)) in the plasma, as well as on acetylcholinesterase (AChE) activity in the cardiac tissue homogenate in the rat. Male Wistar rats were divided into three groups as follows: control group (1 mL 0.9% NaCl, intraperitoneal (i.p.) injection), DL-Hcy group (8 mmol/kg body mass (b.m.), i.p.) or DL-Hcy TLHC group (8 mmol/kg b.m., i.p.). One hour after administration, the rats were euthanized, whole blood was collected for biochemical analysis, and the heart was excised. Following the i.p. administration of DL-Hcy and DL-Hcy TLHC, the activities of antioxidant enzymes were mostly significantly increased, while plasma malondialdehyde (MDA) was decreased. Administration of DL-Hcy and DL-Hcy TLHC significantly inhibited AChE activity in rat cardiac tissue. Our findings suggest that DL-Hcy and DL-Hcy TLHC exerted prooxidant effects; however, the decrease in MDA points to an inverse response to the increase in antioxidant enzyme activities. While both substances inhibited AChE activity in rat cardiac tissue, DL-Hcy TLHC induced stronger effects than DL-Hcy.",
journal = "Archives of Biological Sciences",
title = "The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat",
volume = "70",
number = "2",
pages = "241-248",
doi = "10.2298/ABS170731041K"
}
Kornjača, D., Živković, V. I., Krstić, D. Z., Čolović, M. B., Đurić, M., Stanković, S., Mutavdžin, S., Jakovljević, V. Lj.,& Đurić, D. M.. (2018). The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat. in Archives of Biological Sciences, 70(2), 241-248.
https://doi.org/10.2298/ABS170731041K
Kornjača D, Živković VI, Krstić DZ, Čolović MB, Đurić M, Stanković S, Mutavdžin S, Jakovljević VL, Đurić DM. The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat. in Archives of Biological Sciences. 2018;70(2):241-248.
doi:10.2298/ABS170731041K .
Kornjača, Duško, Živković, Vladimir I., Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Marko, Stanković, Sanja, Mutavdžin, Slavica, Jakovljević, Vladimir Lj., Đurić, Dragan M., "The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat" in Archives of Biological Sciences, 70, no. 2 (2018):241-248,
https://doi.org/10.2298/ABS170731041K . .
1
1

Advanced-level analysis of spiking EEG activity potentiated by high dietary methionine: contribution of purinergic signaling

Hrnčić, Dragan; Markovic, A. Rasic; Sutulovici, N.; Grubač, Željko; Vorkapici, M.; Ademovici, A.; Čolović, Mirjana B.; Krstić, Danijela Z.; Petrović, B. Rankov; Šušić, Veselinka; Đurić, Dragan M.; Stanojlović, Olivera

(2017)

TY  - CONF
AU  - Hrnčić, Dragan
AU  - Markovic, A. Rasic
AU  - Sutulovici, N.
AU  - Grubač, Željko
AU  - Vorkapici, M.
AU  - Ademovici, A.
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Petrović, B. Rankov
AU  - Šušić, Veselinka
AU  - Đurić, Dragan M.
AU  - Stanojlović, Olivera
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1712
C3  - Acta Physiologica
T1  - Advanced-level analysis of spiking EEG activity potentiated by high dietary methionine: contribution of purinergic signaling
VL  - 221
IS  - SI
SP  - 4
EP  - 4
ER  - 
@conference{
author = "Hrnčić, Dragan and Markovic, A. Rasic and Sutulovici, N. and Grubač, Željko and Vorkapici, M. and Ademovici, A. and Čolović, Mirjana B. and Krstić, Danijela Z. and Petrović, B. Rankov and Šušić, Veselinka and Đurić, Dragan M. and Stanojlović, Olivera",
year = "2017",
journal = "Acta Physiologica",
title = "Advanced-level analysis of spiking EEG activity potentiated by high dietary methionine: contribution of purinergic signaling",
volume = "221",
number = "SI",
pages = "4-4"
}
Hrnčić, D., Markovic, A. R., Sutulovici, N., Grubač, Ž., Vorkapici, M., Ademovici, A., Čolović, M. B., Krstić, D. Z., Petrović, B. R., Šušić, V., Đurić, D. M.,& Stanojlović, O.. (2017). Advanced-level analysis of spiking EEG activity potentiated by high dietary methionine: contribution of purinergic signaling. in Acta Physiologica, 221(SI), 4-4.
Hrnčić D, Markovic AR, Sutulovici N, Grubač Ž, Vorkapici M, Ademovici A, Čolović MB, Krstić DZ, Petrović BR, Šušić V, Đurić DM, Stanojlović O. Advanced-level analysis of spiking EEG activity potentiated by high dietary methionine: contribution of purinergic signaling. in Acta Physiologica. 2017;221(SI):4-4..
Hrnčić, Dragan, Markovic, A. Rasic, Sutulovici, N., Grubač, Željko, Vorkapici, M., Ademovici, A., Čolović, Mirjana B., Krstić, Danijela Z., Petrović, B. Rankov, Šušić, Veselinka, Đurić, Dragan M., Stanojlović, Olivera, "Advanced-level analysis of spiking EEG activity potentiated by high dietary methionine: contribution of purinergic signaling" in Acta Physiologica, 221, no. SI (2017):4-4.

The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach

Bondžić, Aleksandra; Čolović, Mirjana B.; Janjić, Goran V.; Zarić, Božidarka; Petrović, Sandra; Krstić, Danijela Z.; Marzo, Tiziano; Messori, Luigi; Vasić, Vesna M.

(2017)

TY  - JOUR
AU  - Bondžić, Aleksandra
AU  - Čolović, Mirjana B.
AU  - Janjić, Goran V.
AU  - Zarić, Božidarka
AU  - Petrović, Sandra
AU  - Krstić, Danijela Z.
AU  - Marzo, Tiziano
AU  - Messori, Luigi
AU  - Vasić, Vesna M.
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1648
AB  - The in vitro effects of oxo-bridged binuclear gold(III) complexes, i.e., [(bipy2Me)(2)Au-2(mu-O)(2)][PF6](2) (Auoxo6), Au-2[(bipydmb-H)(2)(mu-O)][PF6] (Au(2)bipyC) and [Au-2(phen(2Me))(2)(mu-O)(2)](PF6)(2) (Au(2)phen) on Na/K-ATPase, purified from the porcine cerebral cortex, were investigated. All three studied gold complexes inhibited the enzyme activity in a concentration-dependent manner achieving IC50 values in the low micromolar range. Kinetic analysis suggested an uncompetitive mode of inhibition for Auoxo6 and Au(2)bipyC, and a mixed type one for Au(2)phen. Docking studies indicated that the inhibitory actions of all tested complexes are related to E2-P enzyme conformation binding to ion channel and intracellular part between N and P sub-domain. In addition, Au(2)phen was able to inhibit the enzyme by interacting with its extracellular part as well. Toxic effects of the gold(III) complexes were evaluated in vitro by following lactate dehydrogenase activity in rat brain synaptosomes and incidence of micronuclei and cytokinesis-block proliferation index in cultivated human lymphocytes. All investigated complexes turned out to induce cytogenetic damage consisting of a significant decrease in cell proliferation and an increase in micronuclei in a dose-dependent manner. On the other hand, lactate dehydrogenase activity, an indicator of membrane integrity/viability, was not affected by Auoxo6 and Au(2)bipyC, while Au(2)phen slightly modified its activity.
T2  - Journal of Biological Inorganic Chemistry
T1  - The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach
VL  - 22
IS  - 6
SP  - 819
EP  - 832
DO  - 10.1007/s00775-017-1460-5
ER  - 
@article{
author = "Bondžić, Aleksandra and Čolović, Mirjana B. and Janjić, Goran V. and Zarić, Božidarka and Petrović, Sandra and Krstić, Danijela Z. and Marzo, Tiziano and Messori, Luigi and Vasić, Vesna M.",
year = "2017",
abstract = "The in vitro effects of oxo-bridged binuclear gold(III) complexes, i.e., [(bipy2Me)(2)Au-2(mu-O)(2)][PF6](2) (Auoxo6), Au-2[(bipydmb-H)(2)(mu-O)][PF6] (Au(2)bipyC) and [Au-2(phen(2Me))(2)(mu-O)(2)](PF6)(2) (Au(2)phen) on Na/K-ATPase, purified from the porcine cerebral cortex, were investigated. All three studied gold complexes inhibited the enzyme activity in a concentration-dependent manner achieving IC50 values in the low micromolar range. Kinetic analysis suggested an uncompetitive mode of inhibition for Auoxo6 and Au(2)bipyC, and a mixed type one for Au(2)phen. Docking studies indicated that the inhibitory actions of all tested complexes are related to E2-P enzyme conformation binding to ion channel and intracellular part between N and P sub-domain. In addition, Au(2)phen was able to inhibit the enzyme by interacting with its extracellular part as well. Toxic effects of the gold(III) complexes were evaluated in vitro by following lactate dehydrogenase activity in rat brain synaptosomes and incidence of micronuclei and cytokinesis-block proliferation index in cultivated human lymphocytes. All investigated complexes turned out to induce cytogenetic damage consisting of a significant decrease in cell proliferation and an increase in micronuclei in a dose-dependent manner. On the other hand, lactate dehydrogenase activity, an indicator of membrane integrity/viability, was not affected by Auoxo6 and Au(2)bipyC, while Au(2)phen slightly modified its activity.",
journal = "Journal of Biological Inorganic Chemistry",
title = "The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach",
volume = "22",
number = "6",
pages = "819-832",
doi = "10.1007/s00775-017-1460-5"
}
Bondžić, A., Čolović, M. B., Janjić, G. V., Zarić, B., Petrović, S., Krstić, D. Z., Marzo, T., Messori, L.,& Vasić, V. M.. (2017). The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach. in Journal of Biological Inorganic Chemistry, 22(6), 819-832.
https://doi.org/10.1007/s00775-017-1460-5
Bondžić A, Čolović MB, Janjić GV, Zarić B, Petrović S, Krstić DZ, Marzo T, Messori L, Vasić VM. The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach. in Journal of Biological Inorganic Chemistry. 2017;22(6):819-832.
doi:10.1007/s00775-017-1460-5 .
Bondžić, Aleksandra, Čolović, Mirjana B., Janjić, Goran V., Zarić, Božidarka, Petrović, Sandra, Krstić, Danijela Z., Marzo, Tiziano, Messori, Luigi, Vasić, Vesna M., "The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach" in Journal of Biological Inorganic Chemistry, 22, no. 6 (2017):819-832,
https://doi.org/10.1007/s00775-017-1460-5 . .
3
4
4

Hyperhomocysteinemia Induced By Methionine Nutritional Overload More Promptly Affects Brain Than Heart Cholinergic System Without Affects on Food Intake and Body Mass Gain

Hrncic, Dragan; Rasic-Markovic, Aleksandra; Čolović, Mirjana B.; Krstić, Danijela Z.; Sutulovic, Nikola; Grubac, Zeljko; Susic, Veselinka; Đurić, Dragan M.; Stanojlovic, Olivera

(2017)

TY  - CONF
AU  - Hrncic, Dragan
AU  - Rasic-Markovic, Aleksandra
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Sutulovic, Nikola
AU  - Grubac, Zeljko
AU  - Susic, Veselinka
AU  - Đurić, Dragan M.
AU  - Stanojlovic, Olivera
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7176
C3  - Atherosclerosis
T1  - Hyperhomocysteinemia Induced By Methionine Nutritional Overload More Promptly Affects Brain Than Heart Cholinergic System Without Affects on Food Intake and Body Mass Gain
VL  - 263
SP  - E168
EP  - E168
DO  - 10.1016/j.atherosclerosis.2017.06.536
ER  - 
@conference{
author = "Hrncic, Dragan and Rasic-Markovic, Aleksandra and Čolović, Mirjana B. and Krstić, Danijela Z. and Sutulovic, Nikola and Grubac, Zeljko and Susic, Veselinka and Đurić, Dragan M. and Stanojlovic, Olivera",
year = "2017",
journal = "Atherosclerosis",
title = "Hyperhomocysteinemia Induced By Methionine Nutritional Overload More Promptly Affects Brain Than Heart Cholinergic System Without Affects on Food Intake and Body Mass Gain",
volume = "263",
pages = "E168-E168",
doi = "10.1016/j.atherosclerosis.2017.06.536"
}
Hrncic, D., Rasic-Markovic, A., Čolović, M. B., Krstić, D. Z., Sutulovic, N., Grubac, Z., Susic, V., Đurić, D. M.,& Stanojlovic, O.. (2017). Hyperhomocysteinemia Induced By Methionine Nutritional Overload More Promptly Affects Brain Than Heart Cholinergic System Without Affects on Food Intake and Body Mass Gain. in Atherosclerosis, 263, E168-E168.
https://doi.org/10.1016/j.atherosclerosis.2017.06.536
Hrncic D, Rasic-Markovic A, Čolović MB, Krstić DZ, Sutulovic N, Grubac Z, Susic V, Đurić DM, Stanojlovic O. Hyperhomocysteinemia Induced By Methionine Nutritional Overload More Promptly Affects Brain Than Heart Cholinergic System Without Affects on Food Intake and Body Mass Gain. in Atherosclerosis. 2017;263:E168-E168.
doi:10.1016/j.atherosclerosis.2017.06.536 .
Hrncic, Dragan, Rasic-Markovic, Aleksandra, Čolović, Mirjana B., Krstić, Danijela Z., Sutulovic, Nikola, Grubac, Zeljko, Susic, Veselinka, Đurić, Dragan M., Stanojlovic, Olivera, "Hyperhomocysteinemia Induced By Methionine Nutritional Overload More Promptly Affects Brain Than Heart Cholinergic System Without Affects on Food Intake and Body Mass Gain" in Atherosclerosis, 263 (2017):E168-E168,
https://doi.org/10.1016/j.atherosclerosis.2017.06.536 . .

The Effects of Nitric Oxide Synthase Inhibition Following Acute Administration of Homocysteine on Oxidative Stress Markers in Rat Plasma

Jakovljević-Uzelac, Jovana; Čolović, Mirjana B.; Krstić, Danijela Z.; Đurić, Marko; Đurić, Dragan M.

(2017)

TY  - CONF
AU  - Jakovljević-Uzelac, Jovana
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Đurić, Marko
AU  - Đurić, Dragan M.
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7175
C3  - Atherosclerosis
T1  - The Effects of Nitric Oxide Synthase Inhibition Following Acute Administration of Homocysteine on Oxidative Stress Markers in Rat Plasma
VL  - 263
SP  - E137
EP  - E138
DO  - 10.1016/j.atherosclerosis.2017.06.440
ER  - 
@conference{
author = "Jakovljević-Uzelac, Jovana and Čolović, Mirjana B. and Krstić, Danijela Z. and Đurić, Marko and Đurić, Dragan M.",
year = "2017",
journal = "Atherosclerosis",
title = "The Effects of Nitric Oxide Synthase Inhibition Following Acute Administration of Homocysteine on Oxidative Stress Markers in Rat Plasma",
volume = "263",
pages = "E137-E138",
doi = "10.1016/j.atherosclerosis.2017.06.440"
}
Jakovljević-Uzelac, J., Čolović, M. B., Krstić, D. Z., Đurić, M.,& Đurić, D. M.. (2017). The Effects of Nitric Oxide Synthase Inhibition Following Acute Administration of Homocysteine on Oxidative Stress Markers in Rat Plasma. in Atherosclerosis, 263, E137-E138.
https://doi.org/10.1016/j.atherosclerosis.2017.06.440
Jakovljević-Uzelac J, Čolović MB, Krstić DZ, Đurić M, Đurić DM. The Effects of Nitric Oxide Synthase Inhibition Following Acute Administration of Homocysteine on Oxidative Stress Markers in Rat Plasma. in Atherosclerosis. 2017;263:E137-E138.
doi:10.1016/j.atherosclerosis.2017.06.440 .
Jakovljević-Uzelac, Jovana, Čolović, Mirjana B., Krstić, Danijela Z., Đurić, Marko, Đurić, Dragan M., "The Effects of Nitric Oxide Synthase Inhibition Following Acute Administration of Homocysteine on Oxidative Stress Markers in Rat Plasma" in Atherosclerosis, 263 (2017):E137-E138,
https://doi.org/10.1016/j.atherosclerosis.2017.06.440 . .

Toxicity evaluation of two polyoxotungstates with anti-acetylcholinesterase activity

Čolović, Mirjana B.; Medic, Branislava; Cetkovic, Mila; Kravić-Stevović, Tamara K.; Stojanovic, Marko; Ayass, Wassim W.; Mougharbel, Ali S.; Radenković, Miroslav; Prostran, Milica; Kortz, Ulrich; Krstić, Danijela Z.

(2017)

TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Medic, Branislava
AU  - Cetkovic, Mila
AU  - Kravić-Stevović, Tamara K.
AU  - Stojanovic, Marko
AU  - Ayass, Wassim W.
AU  - Mougharbel, Ali S.
AU  - Radenković, Miroslav
AU  - Prostran, Milica
AU  - Kortz, Ulrich
AU  - Krstić, Danijela Z.
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1748
AB  - A toxicity evaluation of two Keggin-type heteropolytungstates, K-7[Ti2PW10O40].6H(2)O and K6H [SiV3W9O40].3H(2)O, with different inhibitory potencies toward acetylcholinesterase activity (IC50 values of 1.04 x 10(-6) and 4.80 x 10(-4) mol/L, respectively) was performed. Wistar albino rats were orally treated with single doses (5 and 50 mg/kg) of both investigated compounds. The biochemical parameters of renal (serum urea and creatinine) and liver function (direct and total bilirubin, alanine transaminase, and aspartate aminotransferase) were determined after 24 h and 14 days. A histopathological analysis of liver tissue was carried out 14 days after the polyoxotungstate administration. Both applied doses of the investigated compounds did not induce statistically significant alterations of the renal function markers. However, the polyoxotungstate treatment caused an increase in the activities of serum alanine transaminase and aspartate aminotransferase in a time- and concentration -dependent manner, although statistically significant changes in bilirubin concentrations were not observed. Furthermore, the detected hepatotoxic effect was confirmed by histhopathological analysis that suggested some reversible liver tissue damage two weeks after the treatment, especially in the case of K6H [SiV3W9O40]-3H(2)O. Accordingly, the toxicity of these two polyoxotungstates with anti-acetylcholinesterase effect cannot be considered as a severe one, but their potential clinical application would require a more complex toxicological study.
T2  - Toxicology and Applied Pharmacology
T1  - Toxicity evaluation of two polyoxotungstates with anti-acetylcholinesterase activity
VL  - 333
SP  - 68
EP  - 75
DO  - 10.1016/j.taap.2017.08.010
ER  - 
@article{
author = "Čolović, Mirjana B. and Medic, Branislava and Cetkovic, Mila and Kravić-Stevović, Tamara K. and Stojanovic, Marko and Ayass, Wassim W. and Mougharbel, Ali S. and Radenković, Miroslav and Prostran, Milica and Kortz, Ulrich and Krstić, Danijela Z.",
year = "2017",
abstract = "A toxicity evaluation of two Keggin-type heteropolytungstates, K-7[Ti2PW10O40].6H(2)O and K6H [SiV3W9O40].3H(2)O, with different inhibitory potencies toward acetylcholinesterase activity (IC50 values of 1.04 x 10(-6) and 4.80 x 10(-4) mol/L, respectively) was performed. Wistar albino rats were orally treated with single doses (5 and 50 mg/kg) of both investigated compounds. The biochemical parameters of renal (serum urea and creatinine) and liver function (direct and total bilirubin, alanine transaminase, and aspartate aminotransferase) were determined after 24 h and 14 days. A histopathological analysis of liver tissue was carried out 14 days after the polyoxotungstate administration. Both applied doses of the investigated compounds did not induce statistically significant alterations of the renal function markers. However, the polyoxotungstate treatment caused an increase in the activities of serum alanine transaminase and aspartate aminotransferase in a time- and concentration -dependent manner, although statistically significant changes in bilirubin concentrations were not observed. Furthermore, the detected hepatotoxic effect was confirmed by histhopathological analysis that suggested some reversible liver tissue damage two weeks after the treatment, especially in the case of K6H [SiV3W9O40]-3H(2)O. Accordingly, the toxicity of these two polyoxotungstates with anti-acetylcholinesterase effect cannot be considered as a severe one, but their potential clinical application would require a more complex toxicological study.",
journal = "Toxicology and Applied Pharmacology",
title = "Toxicity evaluation of two polyoxotungstates with anti-acetylcholinesterase activity",
volume = "333",
pages = "68-75",
doi = "10.1016/j.taap.2017.08.010"
}
Čolović, M. B., Medic, B., Cetkovic, M., Kravić-Stevović, T. K., Stojanovic, M., Ayass, W. W., Mougharbel, A. S., Radenković, M., Prostran, M., Kortz, U.,& Krstić, D. Z.. (2017). Toxicity evaluation of two polyoxotungstates with anti-acetylcholinesterase activity. in Toxicology and Applied Pharmacology, 333, 68-75.
https://doi.org/10.1016/j.taap.2017.08.010
Čolović MB, Medic B, Cetkovic M, Kravić-Stevović TK, Stojanovic M, Ayass WW, Mougharbel AS, Radenković M, Prostran M, Kortz U, Krstić DZ. Toxicity evaluation of two polyoxotungstates with anti-acetylcholinesterase activity. in Toxicology and Applied Pharmacology. 2017;333:68-75.
doi:10.1016/j.taap.2017.08.010 .
Čolović, Mirjana B., Medic, Branislava, Cetkovic, Mila, Kravić-Stevović, Tamara K., Stojanovic, Marko, Ayass, Wassim W., Mougharbel, Ali S., Radenković, Miroslav, Prostran, Milica, Kortz, Ulrich, Krstić, Danijela Z., "Toxicity evaluation of two polyoxotungstates with anti-acetylcholinesterase activity" in Toxicology and Applied Pharmacology, 333 (2017):68-75,
https://doi.org/10.1016/j.taap.2017.08.010 . .
7
8
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9

Modulation of acetylcholinesterase activity induced by polyoxotungstates

Čolović, Mirjana B.; Bondžić, Aleksandra; Kortz, U.; Vasić, Vesna M.; Krstić, Danijela Z.

(Society of Physical Chemists of Serbia, 2016)

TY  - CONF
AU  - Čolović, Mirjana B.
AU  - Bondžić, Aleksandra
AU  - Kortz, U.
AU  - Vasić, Vesna M.
AU  - Krstić, Danijela Z.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9208
AB  - The in  vitroinfluence  of  five  polyoxotungstates  containing  various  central atoms    on    acetylcholinesterase    (AChE)    activity    was    investigated. K6[PV3W9O40] × 3H2O, K6H2[TiW11CoO40] × 13H2O, (NH4)14[NaP5W30O110]    ×    31H2O,    K7[SiV3W9O40]    ×    10H2O,    and K7[Ti2PW10O40]   induced   the   enzyme   inhibition   in   a   concentration-dependent  manner.  Inhibitory  power  of  the  investigated  compounds  was evaluated  using  IC50values.  K7[SiV3W9O40]  ×  10H2O  affected  AChE activity with lowest potency (IC50 = 4.80 × 10-4mol/L). K6H2[TiW11CoO40] ×  13H2O  and  K7[Ti2PW10O40]  exhibited  high  affinity  toward  the  enzyme, inducing half-maximuminhibition at micromolar concentrations (1.14 × 10-6and 1.04 × 10-6mol/L, respectively), while the same effect was achieved in the  presence  of  about  fifty  times  higher  concentration  of  K6[PV3W9O40]  × 3H2O.  Finally,  (NH4)14[NaP5W30O110]  ×  31H2O  was  foundas  the  most potent   inhibitor   of   AChE   activity   (IC50 = 6.36   ×   10-7mol/L),   and consequently the most promising candidate for the treatment of neurological diseases associated with acetylcholine leakage.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
T1  - Modulation of acetylcholinesterase activity induced by polyoxotungstates
SP  - 451
EP  - 454
ER  - 
@conference{
author = "Čolović, Mirjana B. and Bondžić, Aleksandra and Kortz, U. and Vasić, Vesna M. and Krstić, Danijela Z.",
year = "2016",
abstract = "The in  vitroinfluence  of  five  polyoxotungstates  containing  various  central atoms    on    acetylcholinesterase    (AChE)    activity    was    investigated. K6[PV3W9O40] × 3H2O, K6H2[TiW11CoO40] × 13H2O, (NH4)14[NaP5W30O110]    ×    31H2O,    K7[SiV3W9O40]    ×    10H2O,    and K7[Ti2PW10O40]   induced   the   enzyme   inhibition   in   a   concentration-dependent  manner.  Inhibitory  power  of  the  investigated  compounds  was evaluated  using  IC50values.  K7[SiV3W9O40]  ×  10H2O  affected  AChE activity with lowest potency (IC50 = 4.80 × 10-4mol/L). K6H2[TiW11CoO40] ×  13H2O  and  K7[Ti2PW10O40]  exhibited  high  affinity  toward  the  enzyme, inducing half-maximuminhibition at micromolar concentrations (1.14 × 10-6and 1.04 × 10-6mol/L, respectively), while the same effect was achieved in the  presence  of  about  fifty  times  higher  concentration  of  K6[PV3W9O40]  × 3H2O.  Finally,  (NH4)14[NaP5W30O110]  ×  31H2O  was  foundas  the  most potent   inhibitor   of   AChE   activity   (IC50 = 6.36   ×   10-7mol/L),   and consequently the most promising candidate for the treatment of neurological diseases associated with acetylcholine leakage.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry",
title = "Modulation of acetylcholinesterase activity induced by polyoxotungstates",
pages = "451-454"
}
Čolović, M. B., Bondžić, A., Kortz, U., Vasić, V. M.,& Krstić, D. Z.. (2016). Modulation of acetylcholinesterase activity induced by polyoxotungstates. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 451-454.
Čolović MB, Bondžić A, Kortz U, Vasić VM, Krstić DZ. Modulation of acetylcholinesterase activity induced by polyoxotungstates. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry. 2016;:451-454..
Čolović, Mirjana B., Bondžić, Aleksandra, Kortz, U., Vasić, Vesna M., Krstić, Danijela Z., "Modulation of acetylcholinesterase activity induced by polyoxotungstates" in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry (2016):451-454.

A combined crystallographic analysis and ab initio calculations to interpret the reactivity of functionalized hexavanadates and their inhibitor potency toward Na+/K+-ATPase

Xu, Xiao; Bošnjaković-Pavlović, Nada; Čolović, Mirjana B.; Krstić, Danijela Z.; Vasić, Vesna M.; Gillet, Jean-Michel; Wu, Pingfan; Wei, Yongge; Spasojević-de Bire, Anne

(2016)

TY  - JOUR
AU  - Xu, Xiao
AU  - Bošnjaković-Pavlović, Nada
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Vasić, Vesna M.
AU  - Gillet, Jean-Michel
AU  - Wu, Pingfan
AU  - Wei, Yongge
AU  - Spasojević-de Bire, Anne
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1158
AB  - In vitro influence of five synthesized functionalized hexavanadates (V-6) on commercial porcine cerebral cortex Na+/K+-ATPase activity has been studied. Dose dependent Na+/K+-ATPase inhibition was obtained for all investigated compounds. Calculated half maximal inhibitory concentration IC50 values, in mol/L, for Na+/K+-ATPase were 7.6 x 10(-5), 1.8 x 10(-5), 2.9 x 10(-5), 5.5 x 10(-5) for functionalized hexavanadates (V-6) with tetrabutylammonium (TBA) [V-6-CH3][TBA](2), [V-6-NO2][TBA](2), [V-6-OH][TBA](2) and [V-6-C-3][TBA](2) respectively. [V-6-OH][Na](2) inhibited Na+/K+-ATPase activity up to 30% at maximal investigated concentration 1 x 10(-3) mol/L. This reactivity has been interpreted using a study of the non-covalent interactions of functionalized hexavanadate hybrids through Cambridge Structural Database (CSD) analysis. Bibliographic searching has led to 18 different structures and 99 contacts. We have observed that C-H center dot center dot center dot O contacts consolidate the structures. We have also performed density functional theory (DFT) calculations and have determined electrostatic potential values at the molecular surface on a series of functionalized V-6. These results enlightened their chemical reactivity and their potential biological applications such as the inhibition of the ATPase. (C) 2016 Elsevier Inc. All rights reserved.
T2  - Journal of Inorganic Biochemistry
T1  - A combined crystallographic analysis and ab initio calculations to interpret the reactivity of functionalized hexavanadates and their inhibitor potency toward Na+/K+-ATPase
VL  - 161
SP  - 27
EP  - 36
DO  - 10.1016/j.jinorgbio.2016.04.029
ER  - 
@article{
author = "Xu, Xiao and Bošnjaković-Pavlović, Nada and Čolović, Mirjana B. and Krstić, Danijela Z. and Vasić, Vesna M. and Gillet, Jean-Michel and Wu, Pingfan and Wei, Yongge and Spasojević-de Bire, Anne",
year = "2016",
abstract = "In vitro influence of five synthesized functionalized hexavanadates (V-6) on commercial porcine cerebral cortex Na+/K+-ATPase activity has been studied. Dose dependent Na+/K+-ATPase inhibition was obtained for all investigated compounds. Calculated half maximal inhibitory concentration IC50 values, in mol/L, for Na+/K+-ATPase were 7.6 x 10(-5), 1.8 x 10(-5), 2.9 x 10(-5), 5.5 x 10(-5) for functionalized hexavanadates (V-6) with tetrabutylammonium (TBA) [V-6-CH3][TBA](2), [V-6-NO2][TBA](2), [V-6-OH][TBA](2) and [V-6-C-3][TBA](2) respectively. [V-6-OH][Na](2) inhibited Na+/K+-ATPase activity up to 30% at maximal investigated concentration 1 x 10(-3) mol/L. This reactivity has been interpreted using a study of the non-covalent interactions of functionalized hexavanadate hybrids through Cambridge Structural Database (CSD) analysis. Bibliographic searching has led to 18 different structures and 99 contacts. We have observed that C-H center dot center dot center dot O contacts consolidate the structures. We have also performed density functional theory (DFT) calculations and have determined electrostatic potential values at the molecular surface on a series of functionalized V-6. These results enlightened their chemical reactivity and their potential biological applications such as the inhibition of the ATPase. (C) 2016 Elsevier Inc. All rights reserved.",
journal = "Journal of Inorganic Biochemistry",
title = "A combined crystallographic analysis and ab initio calculations to interpret the reactivity of functionalized hexavanadates and their inhibitor potency toward Na+/K+-ATPase",
volume = "161",
pages = "27-36",
doi = "10.1016/j.jinorgbio.2016.04.029"
}
Xu, X., Bošnjaković-Pavlović, N., Čolović, M. B., Krstić, D. Z., Vasić, V. M., Gillet, J., Wu, P., Wei, Y.,& Spasojević-de Bire, A.. (2016). A combined crystallographic analysis and ab initio calculations to interpret the reactivity of functionalized hexavanadates and their inhibitor potency toward Na+/K+-ATPase. in Journal of Inorganic Biochemistry, 161, 27-36.
https://doi.org/10.1016/j.jinorgbio.2016.04.029
Xu X, Bošnjaković-Pavlović N, Čolović MB, Krstić DZ, Vasić VM, Gillet J, Wu P, Wei Y, Spasojević-de Bire A. A combined crystallographic analysis and ab initio calculations to interpret the reactivity of functionalized hexavanadates and their inhibitor potency toward Na+/K+-ATPase. in Journal of Inorganic Biochemistry. 2016;161:27-36.
doi:10.1016/j.jinorgbio.2016.04.029 .
Xu, Xiao, Bošnjaković-Pavlović, Nada, Čolović, Mirjana B., Krstić, Danijela Z., Vasić, Vesna M., Gillet, Jean-Michel, Wu, Pingfan, Wei, Yongge, Spasojević-de Bire, Anne, "A combined crystallographic analysis and ab initio calculations to interpret the reactivity of functionalized hexavanadates and their inhibitor potency toward Na+/K+-ATPase" in Journal of Inorganic Biochemistry, 161 (2016):27-36,
https://doi.org/10.1016/j.jinorgbio.2016.04.029 . .
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The effect of subchronic supplementation with folic acid and L-arginine on homocysteine-induced seizures

Rašić-Marković, Aleksandra; Hrnčić, Dragan; Krstić, Danijela Z.; Čolović, Mirjana B.; Djuric, E.; Rankov-Petrovic, B.; Šušić, Veselinka; Stanojlović, Olivera; Đurić, Dragan M.

(2016)

TY  - JOUR
AU  - Rašić-Marković, Aleksandra
AU  - Hrnčić, Dragan
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Djuric, E.
AU  - Rankov-Petrovic, B.
AU  - Šušić, Veselinka
AU  - Stanojlović, Olivera
AU  - Đurić, Dragan M.
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7114
AB  - The aim of the present study was to examine the effect of subchronic co-administration of folic acid (F) and L-arginine (A) on behavioural and electroencephalographic (EEG) characteristics of DL homocysteine thiolactone (H) induced seizures in adult rats. The activity of membrane ATPases in different brain regions were also investigated. Rats were treated with F, A, or vehicle for 15 days (regimen: F 5 mg/kg + A 500 mg/kg (F5A500); F 10 mg/kg + A 300 mg/kg (F10A300)). Seizures were elicited by convulsive dose of H (H, F5A500H, F10A300H) Subchronic supplementation with F and A did not affect seizure incidence, number of seizure episodes, and severity in F5A500H and F10A300H groups vs. H group. However, a tendency to increase latency and decrease the number of seizure episodes was noticed in the F10A300H group. EEG mean spectral power densities during ictal periods were significantly lower in F10A300H vs. H group. The activity of Na+/K+-ATPase and Mg2+-ATPase was significantly increased in almost all examined structures in rats treated with F and A. We can conclude that subchronic supplementation with folic acid and L-arginine has an antiepileptic effect in DL homocysteine thiolactone induced epilepsy.
T2  - Canadian Journal of Physiology and Pharmacology
T1  - The effect of subchronic supplementation with folic acid and L-arginine on homocysteine-induced seizures
VL  - 94
IS  - 10
SP  - 1083
EP  - 1089
DO  - 10.1139/cjpp-2016-0076
ER  - 
@article{
author = "Rašić-Marković, Aleksandra and Hrnčić, Dragan and Krstić, Danijela Z. and Čolović, Mirjana B. and Djuric, E. and Rankov-Petrovic, B. and Šušić, Veselinka and Stanojlović, Olivera and Đurić, Dragan M.",
year = "2016",
abstract = "The aim of the present study was to examine the effect of subchronic co-administration of folic acid (F) and L-arginine (A) on behavioural and electroencephalographic (EEG) characteristics of DL homocysteine thiolactone (H) induced seizures in adult rats. The activity of membrane ATPases in different brain regions were also investigated. Rats were treated with F, A, or vehicle for 15 days (regimen: F 5 mg/kg + A 500 mg/kg (F5A500); F 10 mg/kg + A 300 mg/kg (F10A300)). Seizures were elicited by convulsive dose of H (H, F5A500H, F10A300H) Subchronic supplementation with F and A did not affect seizure incidence, number of seizure episodes, and severity in F5A500H and F10A300H groups vs. H group. However, a tendency to increase latency and decrease the number of seizure episodes was noticed in the F10A300H group. EEG mean spectral power densities during ictal periods were significantly lower in F10A300H vs. H group. The activity of Na+/K+-ATPase and Mg2+-ATPase was significantly increased in almost all examined structures in rats treated with F and A. We can conclude that subchronic supplementation with folic acid and L-arginine has an antiepileptic effect in DL homocysteine thiolactone induced epilepsy.",
journal = "Canadian Journal of Physiology and Pharmacology",
title = "The effect of subchronic supplementation with folic acid and L-arginine on homocysteine-induced seizures",
volume = "94",
number = "10",
pages = "1083-1089",
doi = "10.1139/cjpp-2016-0076"
}
Rašić-Marković, A., Hrnčić, D., Krstić, D. Z., Čolović, M. B., Djuric, E., Rankov-Petrovic, B., Šušić, V., Stanojlović, O.,& Đurić, D. M.. (2016). The effect of subchronic supplementation with folic acid and L-arginine on homocysteine-induced seizures. in Canadian Journal of Physiology and Pharmacology, 94(10), 1083-1089.
https://doi.org/10.1139/cjpp-2016-0076
Rašić-Marković A, Hrnčić D, Krstić DZ, Čolović MB, Djuric E, Rankov-Petrovic B, Šušić V, Stanojlović O, Đurić DM. The effect of subchronic supplementation with folic acid and L-arginine on homocysteine-induced seizures. in Canadian Journal of Physiology and Pharmacology. 2016;94(10):1083-1089.
doi:10.1139/cjpp-2016-0076 .
Rašić-Marković, Aleksandra, Hrnčić, Dragan, Krstić, Danijela Z., Čolović, Mirjana B., Djuric, E., Rankov-Petrovic, B., Šušić, Veselinka, Stanojlović, Olivera, Đurić, Dragan M., "The effect of subchronic supplementation with folic acid and L-arginine on homocysteine-induced seizures" in Canadian Journal of Physiology and Pharmacology, 94, no. 10 (2016):1083-1089,
https://doi.org/10.1139/cjpp-2016-0076 . .
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10

Biochemical Markers of Renal Function

Krstić, Danijela Z.; Tomic, Nenad; Radosavljevic, Branimir; Avramović, Nataša; Dragutinovic, Vesna; Skodric, Sanja Radojevic; Čolović, Mirjana B.

(2016)

TY  - JOUR
AU  - Krstić, Danijela Z.
AU  - Tomic, Nenad
AU  - Radosavljevic, Branimir
AU  - Avramović, Nataša
AU  - Dragutinovic, Vesna
AU  - Skodric, Sanja Radojevic
AU  - Čolović, Mirjana B.
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1200
AB  - Kidney damage can be induced by ischemia, autoimmune diseases, hypertension, allograft rejection, metabolic or genetic disorders, infections or toxins. The influence of these factors could result in acute kidney injury (AKI) defined as an unexpected decrease in urine output or renal function, or encourage the development of chronic kidney disease (CKD). Biomarkers of renal function, measured in urine and serum, are in increasing use in order to estimate the severity and nature of kidney injury, and consequently apply appropriate therapy and improve patient management. The determined values of biomarkers can suggest the potential risk of kidney disease and the type of renal injury, predict the disease progression, as well as be helpful for assessing the response to an applied therapy. Although novel biomarkers are in practical use, serum creatinine, the indicator of glomerular filtration rate is still the most frequently used biomarker of renal function despite its known limitations. In recent decades, numerous studies resulted in discovering urinary and serum proteins that can serve as biomarkers for early and accurate detection of AKI and its development, as well as the identification of CKD. This review gives an overview of the most important renal biomarkers investigated in kidney diseases, classified in following types: functional biomarkers, up-regulated proteins, enzymes, and cycle arrest biomarkers. It describes their properties, physiological roles, and discusses the utility of these molecules in different clinical settings.
T2  - Current Medicinal Chemistry
T1  - Biochemical Markers of Renal Function
VL  - 23
IS  - 19
SP  - 2018
EP  - 2040
DO  - 10.2174/0929867323666160115130241
ER  - 
@article{
author = "Krstić, Danijela Z. and Tomic, Nenad and Radosavljevic, Branimir and Avramović, Nataša and Dragutinovic, Vesna and Skodric, Sanja Radojevic and Čolović, Mirjana B.",
year = "2016",
abstract = "Kidney damage can be induced by ischemia, autoimmune diseases, hypertension, allograft rejection, metabolic or genetic disorders, infections or toxins. The influence of these factors could result in acute kidney injury (AKI) defined as an unexpected decrease in urine output or renal function, or encourage the development of chronic kidney disease (CKD). Biomarkers of renal function, measured in urine and serum, are in increasing use in order to estimate the severity and nature of kidney injury, and consequently apply appropriate therapy and improve patient management. The determined values of biomarkers can suggest the potential risk of kidney disease and the type of renal injury, predict the disease progression, as well as be helpful for assessing the response to an applied therapy. Although novel biomarkers are in practical use, serum creatinine, the indicator of glomerular filtration rate is still the most frequently used biomarker of renal function despite its known limitations. In recent decades, numerous studies resulted in discovering urinary and serum proteins that can serve as biomarkers for early and accurate detection of AKI and its development, as well as the identification of CKD. This review gives an overview of the most important renal biomarkers investigated in kidney diseases, classified in following types: functional biomarkers, up-regulated proteins, enzymes, and cycle arrest biomarkers. It describes their properties, physiological roles, and discusses the utility of these molecules in different clinical settings.",
journal = "Current Medicinal Chemistry",
title = "Biochemical Markers of Renal Function",
volume = "23",
number = "19",
pages = "2018-2040",
doi = "10.2174/0929867323666160115130241"
}
Krstić, D. Z., Tomic, N., Radosavljevic, B., Avramović, N., Dragutinovic, V., Skodric, S. R.,& Čolović, M. B.. (2016). Biochemical Markers of Renal Function. in Current Medicinal Chemistry, 23(19), 2018-2040.
https://doi.org/10.2174/0929867323666160115130241
Krstić DZ, Tomic N, Radosavljevic B, Avramović N, Dragutinovic V, Skodric SR, Čolović MB. Biochemical Markers of Renal Function. in Current Medicinal Chemistry. 2016;23(19):2018-2040.
doi:10.2174/0929867323666160115130241 .
Krstić, Danijela Z., Tomic, Nenad, Radosavljevic, Branimir, Avramović, Nataša, Dragutinovic, Vesna, Skodric, Sanja Radojevic, Čolović, Mirjana B., "Biochemical Markers of Renal Function" in Current Medicinal Chemistry, 23, no. 19 (2016):2018-2040,
https://doi.org/10.2174/0929867323666160115130241 . .
16
16
13

The effect of subchronic supplementation with folic acid on homocysteine induced seizures

Rašić-Marković, Aleksandra; Rankov-Petrović, B.; Hrnčić, Dragan; Krstić, Danijela Z.; Čolović, Mirjana B.; Macut, Dj; Đurić, Dragan M.; Stanojlović, Olivera

(2015)

TY  - JOUR
AU  - Rašić-Marković, Aleksandra
AU  - Rankov-Petrović, B.
AU  - Hrnčić, Dragan
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Macut, Dj
AU  - Đurić, Dragan M.
AU  - Stanojlović, Olivera
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/619
AB  - Influence of folic acid on the CNS is still unclear. Folate has a neuroprotective effect, while on the other hand excess folate can exacerbate seizures in epileptics. The aim of the present study was to examine the effect of subchronic administration of folic acid on behavioural and electroencephalographic (EEG) characteristics of DL homocysteine thiolactone induced seizures in adult rats. The activity of Na+/K+-ATPase and Mg2+-ATPase in different brain regions was investigated. Adult male Wistar rats were divided into groups: 1. Controls (C, 0.9% NaCl); 2. DL homocysteine-thiolactone 8.0 mmol/kg (H); 3. Subchronic supplementation with folic acid 5 mg/kg for 7 days (F) and 4. Subchronic supplementation with F + single dose of H (FH). Seizure behaviour was assessed by incidence, latency, number and intensity of seizure episodes. Seizure severity was described by a descriptive scale with grades 0-4. For EEG recordings, three gold-plated recording electrodes were implanted into the skull. Subchronic supplementation with folic acid did not affect seizure incidence, median number of seizure episodes and severity in FH, comparison with H (p GT 0.05). The majority of seizure episodes in all groups were of grade 2. There were no significant differences in lethal outcomes at 24 h upon H injection in the FH vs. H group. The activity of Na+/K+-ATPase and Mg2+-ATPase was significantly increased in almost all examined structures in the FH vs. H group. Subchronic folic acid administration did not exacerbate H induced seizures and completely recovered the activity of ATPases.
T2  - Acta Physiologica Hungarica
T1  - The effect of subchronic supplementation with folic acid on homocysteine induced seizures
VL  - 102
IS  - 2
SP  - 151
EP  - 162
DO  - 10.1556/036.102.2015.2.6
ER  - 
@article{
author = "Rašić-Marković, Aleksandra and Rankov-Petrović, B. and Hrnčić, Dragan and Krstić, Danijela Z. and Čolović, Mirjana B. and Macut, Dj and Đurić, Dragan M. and Stanojlović, Olivera",
year = "2015",
abstract = "Influence of folic acid on the CNS is still unclear. Folate has a neuroprotective effect, while on the other hand excess folate can exacerbate seizures in epileptics. The aim of the present study was to examine the effect of subchronic administration of folic acid on behavioural and electroencephalographic (EEG) characteristics of DL homocysteine thiolactone induced seizures in adult rats. The activity of Na+/K+-ATPase and Mg2+-ATPase in different brain regions was investigated. Adult male Wistar rats were divided into groups: 1. Controls (C, 0.9% NaCl); 2. DL homocysteine-thiolactone 8.0 mmol/kg (H); 3. Subchronic supplementation with folic acid 5 mg/kg for 7 days (F) and 4. Subchronic supplementation with F + single dose of H (FH). Seizure behaviour was assessed by incidence, latency, number and intensity of seizure episodes. Seizure severity was described by a descriptive scale with grades 0-4. For EEG recordings, three gold-plated recording electrodes were implanted into the skull. Subchronic supplementation with folic acid did not affect seizure incidence, median number of seizure episodes and severity in FH, comparison with H (p GT 0.05). The majority of seizure episodes in all groups were of grade 2. There were no significant differences in lethal outcomes at 24 h upon H injection in the FH vs. H group. The activity of Na+/K+-ATPase and Mg2+-ATPase was significantly increased in almost all examined structures in the FH vs. H group. Subchronic folic acid administration did not exacerbate H induced seizures and completely recovered the activity of ATPases.",
journal = "Acta Physiologica Hungarica",
title = "The effect of subchronic supplementation with folic acid on homocysteine induced seizures",
volume = "102",
number = "2",
pages = "151-162",
doi = "10.1556/036.102.2015.2.6"
}
Rašić-Marković, A., Rankov-Petrović, B., Hrnčić, D., Krstić, D. Z., Čolović, M. B., Macut, D., Đurić, D. M.,& Stanojlović, O.. (2015). The effect of subchronic supplementation with folic acid on homocysteine induced seizures. in Acta Physiologica Hungarica, 102(2), 151-162.
https://doi.org/10.1556/036.102.2015.2.6
Rašić-Marković A, Rankov-Petrović B, Hrnčić D, Krstić DZ, Čolović MB, Macut D, Đurić DM, Stanojlović O. The effect of subchronic supplementation with folic acid on homocysteine induced seizures. in Acta Physiologica Hungarica. 2015;102(2):151-162.
doi:10.1556/036.102.2015.2.6 .
Rašić-Marković, Aleksandra, Rankov-Petrović, B., Hrnčić, Dragan, Krstić, Danijela Z., Čolović, Mirjana B., Macut, Dj, Đurić, Dragan M., Stanojlović, Olivera, "The effect of subchronic supplementation with folic acid on homocysteine induced seizures" in Acta Physiologica Hungarica, 102, no. 2 (2015):151-162,
https://doi.org/10.1556/036.102.2015.2.6 . .
4
5
5

In vitro evaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes

Čolović, Mirjana B.; Vasić, Vesna M.; Avramović, Nataša; Gajic, Milan M.; Đurić, Dragan M.; Krstić, Danijela Z.

(2015)

TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Vasić, Vesna M.
AU  - Avramović, Nataša
AU  - Gajic, Milan M.
AU  - Đurić, Dragan M.
AU  - Krstić, Danijela Z.
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/409
AB  - Although primary toxic action of organophosphorous insecticides is associated with acetylcholinesterase inhibition, later studies suggest that oxidative stress may be responsible for induced organophosphates toxicity. These studies mostly include thio forms, while the effects of their metabolites/ degradation products have been less investigated. Therefore, this paper studies the toxic effects of diazinon degradation products, diazoxon and 2-isopropyl-6-methyl-4-pyrimidinol, and compares them with the toxic potential of the parent compound. The toxicity induced by various concentrations of the investigated compounds was in vitro evaluated by the activities of acetylcholinesterase, ATPases, antioxidant defense enzymes and lactate dehydrogenase, and malondialdehyde level in rat brain synaptosomes. Diazinon inhibited acetylcholinesterase and Na+/K+-ATPase in dose-dependent manner, while the inhibition of ecto-ATPase activity was less than 15% at all investigated concentrations. It did not demonstrate noteworthy prooxidative properties causing increase (up to 10%) in antioxidant enzymes activity and malondialdehyde level, as a marker of lipid peroxidation. Diazinon oxidation product, diazoxon was found as the most toxic investigated compound. Beside the expected strong inhibitory effect on acetylcholinesterase, it induced dose-dependent and almost complete inhibition of Na+/K+-ATPase and ecto-ATPase at the highest investigated concentration (0.1 mM). Increasing diazoxon concentrations activated catalase (up to 30%), superoxide dismutase (up to 50%), glutathione peroxidase (up to 30%), and significantly increased malondialdehyde level (up to 50%). The investigated hydrolysis product of diazinon, 2-isopropyl-6-methyl-4-pyrimidinol did not remarkably alter the activities of acetylcholinesterase, Na+/K+-ATPase, catalase, glutathione peroxidase and lipid peroxidation level (up to about 10%). Although this diazinon metabolite has been known as non toxic, it induced superoxide dismutase stimulation up to 30%. Finally, even high concentrations of both diazinon and its metabolites did noticeably affect lactate dehydrogenase activity as a marker of synaptosomal integrity. The changes in investigated biochemical parameters in rat brain synaptosomes could serve as indicators of toxicity due to the exposure to thio organophosphates and/or their break-down products. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
T2  - Toxicology Letters
T1  - In vitro evaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes
VL  - 233
IS  - 1
SP  - 29
EP  - 37
DO  - 10.1016/j.toxlet.2015.01.003
ER  - 
@article{
author = "Čolović, Mirjana B. and Vasić, Vesna M. and Avramović, Nataša and Gajic, Milan M. and Đurić, Dragan M. and Krstić, Danijela Z.",
year = "2015",
abstract = "Although primary toxic action of organophosphorous insecticides is associated with acetylcholinesterase inhibition, later studies suggest that oxidative stress may be responsible for induced organophosphates toxicity. These studies mostly include thio forms, while the effects of their metabolites/ degradation products have been less investigated. Therefore, this paper studies the toxic effects of diazinon degradation products, diazoxon and 2-isopropyl-6-methyl-4-pyrimidinol, and compares them with the toxic potential of the parent compound. The toxicity induced by various concentrations of the investigated compounds was in vitro evaluated by the activities of acetylcholinesterase, ATPases, antioxidant defense enzymes and lactate dehydrogenase, and malondialdehyde level in rat brain synaptosomes. Diazinon inhibited acetylcholinesterase and Na+/K+-ATPase in dose-dependent manner, while the inhibition of ecto-ATPase activity was less than 15% at all investigated concentrations. It did not demonstrate noteworthy prooxidative properties causing increase (up to 10%) in antioxidant enzymes activity and malondialdehyde level, as a marker of lipid peroxidation. Diazinon oxidation product, diazoxon was found as the most toxic investigated compound. Beside the expected strong inhibitory effect on acetylcholinesterase, it induced dose-dependent and almost complete inhibition of Na+/K+-ATPase and ecto-ATPase at the highest investigated concentration (0.1 mM). Increasing diazoxon concentrations activated catalase (up to 30%), superoxide dismutase (up to 50%), glutathione peroxidase (up to 30%), and significantly increased malondialdehyde level (up to 50%). The investigated hydrolysis product of diazinon, 2-isopropyl-6-methyl-4-pyrimidinol did not remarkably alter the activities of acetylcholinesterase, Na+/K+-ATPase, catalase, glutathione peroxidase and lipid peroxidation level (up to about 10%). Although this diazinon metabolite has been known as non toxic, it induced superoxide dismutase stimulation up to 30%. Finally, even high concentrations of both diazinon and its metabolites did noticeably affect lactate dehydrogenase activity as a marker of synaptosomal integrity. The changes in investigated biochemical parameters in rat brain synaptosomes could serve as indicators of toxicity due to the exposure to thio organophosphates and/or their break-down products. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
journal = "Toxicology Letters",
title = "In vitro evaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes",
volume = "233",
number = "1",
pages = "29-37",
doi = "10.1016/j.toxlet.2015.01.003"
}
Čolović, M. B., Vasić, V. M., Avramović, N., Gajic, M. M., Đurić, D. M.,& Krstić, D. Z.. (2015). In vitro evaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes. in Toxicology Letters, 233(1), 29-37.
https://doi.org/10.1016/j.toxlet.2015.01.003
Čolović MB, Vasić VM, Avramović N, Gajic MM, Đurić DM, Krstić DZ. In vitro evaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes. in Toxicology Letters. 2015;233(1):29-37.
doi:10.1016/j.toxlet.2015.01.003 .
Čolović, Mirjana B., Vasić, Vesna M., Avramović, Nataša, Gajic, Milan M., Đurić, Dragan M., Krstić, Danijela Z., "In vitro evaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes" in Toxicology Letters, 233, no. 1 (2015):29-37,
https://doi.org/10.1016/j.toxlet.2015.01.003 . .
28
25
33

Bioanalitičke metode za detekciju i evaluaciju toksičnosti organo-tiofosfatnih insekticida i proizvoda njihove degradacije

Čolović, Mirjana B.

(Универзитет у Београду, Технолошко-металуршки факултет, 2014)

TY  - THES
AU  - Čolović, Mirjana B.
PY  - 2014
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=3941
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:13247/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=46751503
UR  - http://nardus.mpn.gov.rs/123456789/6586
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7306
AB  - U ovoj tezi su ispitani toksični efekti organo-tiofosfatnih insekticida i proizvoda njihove degradacije primenom različitih metoda kojima se mere parametri toksičnosti. Degradacijom organo-tiofosfatnih insekticida, do koje dolazi tokom fizičko-hemijskih procesa za uklanjanje organskih zagađivača iz voda, delovanjem faktora životne sredine, tokom dužeg skladištenja preparata insekticida ili sterilizacijom hrane, nastaju različita jedinjenja koja često poseduju jači toksični potencijal od polaznih insekticida. Proizvodi degradacije nisu ciljani analiti tokom kontrole prisustva insekticida, a i njihova detekcija primenom klasičnih analitičkih metoda je skupa, komplikovana i dugotrojna i ne može da predvidi toksične efekte na osnovu identifikovanog sastava. Znatno pogodnija je primena bioeseja koji detektuju prisustvo toksičnih proizvoda degradacije na osnovu promene odgovarajućih parametara i istovremeno daju informaciju o njihovom toksičnom potencijalu.Za procenu neurotoksičnog potencijala ispitivanih organofosfatnih jedinjenja i njihovu detekciju modifikovan je standardni esej na bazi acetilholinesteraze, koji je unapredjen u cilju snižavanja limita detekcije organofosfata. Konstruisan je i protočni biosenzor sa imobilizovanom acetilholinesterazom koja se može koristiti više puta tokom nekoliko meseci, što ovaj sistem čini ekonomičnijim u odnosu na šaržni esej. U cilju povaćanja osetljivosti standardnog eseja i biosenzora na prisustvo organo-tiofosfata, korišćeno je oksidaciono sredstvo, N-bromo sukcinimid koji tio oblike prevodi u okso koji dovode do pada aktivnosti enzima pri znatno nižim koncentracijama. Testirano je i simultano dejstvo organo-tiofosfata i njihovih okso i izo analoga.U tezi su analizirani diazinon, malation, hlorpirifos, organo-tiofosfati koji se primenjuju kao preparati insekticida, i proizvodi njihove oksidacije, hidrolize i izomerizacije.Organo-tiofosfatni insekticid diazinon je tretiran UV svetlošću, koja se koristi za uklanjanje organskih zagađivača u procesima za prečišćavanje voda. Hromatografski je praćena razgradnja polaznog jedinjenja i nastajanje proizvoda hidrolize tokom 115minuta ozračivanja diazinona. Ispitano je neurotoksično, prooksidativno, genotoksično i citotoksično dejstvo ozračenih rastvora, kao i samog diazinona i identifikovanog proizvoda njegove fotohemijske degradacije...
AB  - Toxic effects of organo-thiophosphorous insecticides i their degradation products have been investigated in this dissertation using various methods for the determination of toxicity parameters. Organo-thiophosphorous insecticides can be degraded during physico-chemical processes for the removal of organic pollutants from wastewaters, insecticides storage, food sterilisation, as well as under the influence of environmental factors. The formed degradation products are not the aimed analytes during insecticides control, and their detection using analytical methods is expensive, complicated and longterm. Additionally, toxic effects of the identified compounds cannot be evaluated. Therefore, the application of bioassays, which detect the degradation products on the basis of the modification of appropriate parameters and give the information about their toxic potential, is considerably suitable.The standard acetylcholinesterase assay has been modified for the evaluation of neurotoxic potential of the investigated organophosphorous compounds and the reduction of their limit of detection. Besides, flow injection analysis biosensor based on immobilized acetylcholinesterase has been constructed. The immobilized enzyme can be used more times during several months, making the biosenzor more economical related to the batch system. In order to increase the sensitivity of the biosenzor, N-bromosuccinimide has been used. Actually, this oxidant transforms thio forms of organophosphates to oxo analogs causing the decrease of the enzyme activity at lower concentrations. The simultaneous influence of the organo-thiophosphates and their oxo and iso analogs has been investigated as well.In this thesis, diazinon, malathion, chlorpyrifos-organo-thiophosphates being applied as insecticides, and the products of their oxidation, hydrolysis and isomerisation have been tested.Organo-thiophosphorous insecticide diazinon has been treated with UV light that is applied in purification processes for the removal of organic pollutants from wastewaters. The decomposition of the parent organo-thiophosphate and forming the hydrolysis product during 115 minute irradiation has been monitored usingchromatographic methods. It has been investigated neurotoxic, prooxidative, genotoxic and citotoxic effects of the irradiated diazinon solutions, as well as various concentrations of diazinon and the identified product of diazinon photochemical degradation...
PB  - Универзитет у Београду, Технолошко-металуршки факултет
T2  - Универзитет у Београду
T1  - Bioanalitičke metode za detekciju i evaluaciju toksičnosti organo-tiofosfatnih insekticida i proizvoda njihove degradacije
T1  - Bioanalytical methods for detection and toxicity evaluation of organo-thiophosphorous insecticides and their degradation products
ER  - 
@phdthesis{
author = "Čolović, Mirjana B.",
year = "2014",
abstract = "U ovoj tezi su ispitani toksični efekti organo-tiofosfatnih insekticida i proizvoda njihove degradacije primenom različitih metoda kojima se mere parametri toksičnosti. Degradacijom organo-tiofosfatnih insekticida, do koje dolazi tokom fizičko-hemijskih procesa za uklanjanje organskih zagađivača iz voda, delovanjem faktora životne sredine, tokom dužeg skladištenja preparata insekticida ili sterilizacijom hrane, nastaju različita jedinjenja koja često poseduju jači toksični potencijal od polaznih insekticida. Proizvodi degradacije nisu ciljani analiti tokom kontrole prisustva insekticida, a i njihova detekcija primenom klasičnih analitičkih metoda je skupa, komplikovana i dugotrojna i ne može da predvidi toksične efekte na osnovu identifikovanog sastava. Znatno pogodnija je primena bioeseja koji detektuju prisustvo toksičnih proizvoda degradacije na osnovu promene odgovarajućih parametara i istovremeno daju informaciju o njihovom toksičnom potencijalu.Za procenu neurotoksičnog potencijala ispitivanih organofosfatnih jedinjenja i njihovu detekciju modifikovan je standardni esej na bazi acetilholinesteraze, koji je unapredjen u cilju snižavanja limita detekcije organofosfata. Konstruisan je i protočni biosenzor sa imobilizovanom acetilholinesterazom koja se može koristiti više puta tokom nekoliko meseci, što ovaj sistem čini ekonomičnijim u odnosu na šaržni esej. U cilju povaćanja osetljivosti standardnog eseja i biosenzora na prisustvo organo-tiofosfata, korišćeno je oksidaciono sredstvo, N-bromo sukcinimid koji tio oblike prevodi u okso koji dovode do pada aktivnosti enzima pri znatno nižim koncentracijama. Testirano je i simultano dejstvo organo-tiofosfata i njihovih okso i izo analoga.U tezi su analizirani diazinon, malation, hlorpirifos, organo-tiofosfati koji se primenjuju kao preparati insekticida, i proizvodi njihove oksidacije, hidrolize i izomerizacije.Organo-tiofosfatni insekticid diazinon je tretiran UV svetlošću, koja se koristi za uklanjanje organskih zagađivača u procesima za prečišćavanje voda. Hromatografski je praćena razgradnja polaznog jedinjenja i nastajanje proizvoda hidrolize tokom 115minuta ozračivanja diazinona. Ispitano je neurotoksično, prooksidativno, genotoksično i citotoksično dejstvo ozračenih rastvora, kao i samog diazinona i identifikovanog proizvoda njegove fotohemijske degradacije..., Toxic effects of organo-thiophosphorous insecticides i their degradation products have been investigated in this dissertation using various methods for the determination of toxicity parameters. Organo-thiophosphorous insecticides can be degraded during physico-chemical processes for the removal of organic pollutants from wastewaters, insecticides storage, food sterilisation, as well as under the influence of environmental factors. The formed degradation products are not the aimed analytes during insecticides control, and their detection using analytical methods is expensive, complicated and longterm. Additionally, toxic effects of the identified compounds cannot be evaluated. Therefore, the application of bioassays, which detect the degradation products on the basis of the modification of appropriate parameters and give the information about their toxic potential, is considerably suitable.The standard acetylcholinesterase assay has been modified for the evaluation of neurotoxic potential of the investigated organophosphorous compounds and the reduction of their limit of detection. Besides, flow injection analysis biosensor based on immobilized acetylcholinesterase has been constructed. The immobilized enzyme can be used more times during several months, making the biosenzor more economical related to the batch system. In order to increase the sensitivity of the biosenzor, N-bromosuccinimide has been used. Actually, this oxidant transforms thio forms of organophosphates to oxo analogs causing the decrease of the enzyme activity at lower concentrations. The simultaneous influence of the organo-thiophosphates and their oxo and iso analogs has been investigated as well.In this thesis, diazinon, malathion, chlorpyrifos-organo-thiophosphates being applied as insecticides, and the products of their oxidation, hydrolysis and isomerisation have been tested.Organo-thiophosphorous insecticide diazinon has been treated with UV light that is applied in purification processes for the removal of organic pollutants from wastewaters. The decomposition of the parent organo-thiophosphate and forming the hydrolysis product during 115 minute irradiation has been monitored usingchromatographic methods. It has been investigated neurotoxic, prooxidative, genotoxic and citotoxic effects of the irradiated diazinon solutions, as well as various concentrations of diazinon and the identified product of diazinon photochemical degradation...",
publisher = "Универзитет у Београду, Технолошко-металуршки факултет",
journal = "Универзитет у Београду",
title = "Bioanalitičke metode za detekciju i evaluaciju toksičnosti organo-tiofosfatnih insekticida i proizvoda njihove degradacije, Bioanalytical methods for detection and toxicity evaluation of organo-thiophosphorous insecticides and their degradation products"
}
Čolović, M. B.. (2014). Bioanalitičke metode za detekciju i evaluaciju toksičnosti organo-tiofosfatnih insekticida i proizvoda njihove degradacije. in Универзитет у Београду
Универзитет у Београду, Технолошко-металуршки факултет..
Čolović MB. Bioanalitičke metode za detekciju i evaluaciju toksičnosti organo-tiofosfatnih insekticida i proizvoda njihove degradacije. in Универзитет у Београду. 2014;..
Čolović, Mirjana B., "Bioanalitičke metode za detekciju i evaluaciju toksičnosti organo-tiofosfatnih insekticida i proizvoda njihove degradacije" in Универзитет у Београду (2014).

The effects of diazinon and its degradation products on oxidative stress parameters in rat brain synaptosomes

Čolović, Mirjana B.; Vasić, Vesna M.; Avramović, Nataša; Đurić, Dragan M.; Krstić, Danijela Z.

(Society of Physical Chemists of Serbia, 2014)

TY  - CONF
AU  - Čolović, Mirjana B.
AU  - Vasić, Vesna M.
AU  - Avramović, Nataša
AU  - Đurić, Dragan M.
AU  - Krstić, Danijela Z.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9195
AB  - In vitro evaluation of oxidative stress responses to various concentrations of
diazinon and its degradation products, diazoxon and 2-isopropyl-6-methyl-
4-pyrimidinol (IMP) was investigated by determining antioxidant enzymes
activity (catalase (CAT), superoxide dismutase (SOD) and glutathione
peroxidase (GPx)) and lipid peroxidation level in rat brain synaptosomes.
Diazinon showed negligible prooxidative properties causing increase in
antioxidant enzymes activity and lipid peroxidation level up to 10%.
Increasing concentrations of diazinon oxidation product, diazoxon activated
CAT (up to 20%), SOD (up to 50%), GPx (up to 25%), and significantly
increased the content of lipid peroxidation indicator (up to 50%). The
investigated hydrolysis product of diazinon, IMP did not remarkably
influence the activity of CAT, GPx and lipid peroxidation level (up to 10%),
while it induced SOD stimulation up to 30%.
PB  - Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - The effects of diazinon and its degradation products on oxidative stress parameters in rat brain synaptosomes
VL  - F-24-P
ER  - 
@conference{
author = "Čolović, Mirjana B. and Vasić, Vesna M. and Avramović, Nataša and Đurić, Dragan M. and Krstić, Danijela Z.",
year = "2014",
abstract = "In vitro evaluation of oxidative stress responses to various concentrations of
diazinon and its degradation products, diazoxon and 2-isopropyl-6-methyl-
4-pyrimidinol (IMP) was investigated by determining antioxidant enzymes
activity (catalase (CAT), superoxide dismutase (SOD) and glutathione
peroxidase (GPx)) and lipid peroxidation level in rat brain synaptosomes.
Diazinon showed negligible prooxidative properties causing increase in
antioxidant enzymes activity and lipid peroxidation level up to 10%.
Increasing concentrations of diazinon oxidation product, diazoxon activated
CAT (up to 20%), SOD (up to 50%), GPx (up to 25%), and significantly
increased the content of lipid peroxidation indicator (up to 50%). The
investigated hydrolysis product of diazinon, IMP did not remarkably
influence the activity of CAT, GPx and lipid peroxidation level (up to 10%),
while it induced SOD stimulation up to 30%.",
publisher = "Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "The effects of diazinon and its degradation products on oxidative stress parameters in rat brain synaptosomes",
volume = "F-24-P"
}
Čolović, M. B., Vasić, V. M., Avramović, N., Đurić, D. M.,& Krstić, D. Z.. (2014). The effects of diazinon and its degradation products on oxidative stress parameters in rat brain synaptosomes. in PHYSICAL CHEMISTRY 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., F-24-P.
Čolović MB, Vasić VM, Avramović N, Đurić DM, Krstić DZ. The effects of diazinon and its degradation products on oxidative stress parameters in rat brain synaptosomes. in PHYSICAL CHEMISTRY 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2014;F-24-P..
Čolović, Mirjana B., Vasić, Vesna M., Avramović, Nataša, Đurić, Dragan M., Krstić, Danijela Z., "The effects of diazinon and its degradation products on oxidative stress parameters in rat brain synaptosomes" in PHYSICAL CHEMISTRY 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry, F-24-P (2014).