Lazarević-Pašti, Tamara

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Authority KeyName Variants
orcid::0000-0001-6220-2079
  • Lazarević-Pašti, Tamara (26)
Projects
Studies of enzyme interactions with toxic and pharmacologically active molecules Lithium-ion batteries and fuel cells - research and development
Bilateral project Germany-Serbia, funded by DAAD [Theoretical and experimental development of novel sensor based on graphene composites for the detection of organophosphate pesticides (SeGraPhos)] Carl Tryggers Foundation for Scientific Research
CMST COST Action [CM1203 (PoCheMoN)] COST action CM1203 Polyoxometalate Chemistry for Molecular Nanoscience (PoCheMoN), COST-STSM-ECOST-STSM-CM1203-030416-072554
COST Action [MP1302] Effects of metabolic and nonmetabolic stressors on the expression and action of neuroendocrine regulators of energy homeostasis
Radiosensitivity of human genome Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) Studying climate change and its influence on environment: impacts, adaptation and mitigation
Synthesis, processing and characterization of nanostructured materials for application in the field of energy, mechanical engineering, environmental protection and biomedicine Innovation Fund of the Republic of Serbia [CGS50083]
Ministry of Science and Environmental Protection of the Republic of Serbia Ministry of Science and Technological Development of the Republic of Serbia
Sächsische Aufbaubank (SAB) and the European Social Fund (ESF) - InnoTeam-SimplySafe [100331073] Serbian Academy of Science and Arts
Swedish Research Council (2014-5993) Swiss National Science Foundation (200020-153549)
Swiss National Science Foundation (200020-175800) Swiss National Science Foundation (206021-113149)
Tomsk Polytechnic University Competitiveness Enhancement Program

Author's Bibliography

A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition

Bondžić, Aleksandra; Lazarević-Pašti, Tamara; Leskovac, Andreja; Petrović, Sandra; Čolović, Mirjana B.; Parac Vogt, Tatjana N.; Janjić, Goran V.

(2020)

TY  - JOUR
AU  - Bondžić, Aleksandra
AU  - Lazarević-Pašti, Tamara
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Čolović, Mirjana B.
AU  - Parac Vogt, Tatjana N.
AU  - Janjić, Goran V.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9028
AB  - Acetylcholinesterase (AChE) inhibitors are important in the treatment of neurodegenerative diseases. Two inhibitors,12-tungstosilicic acid (WSiA) and 12-tungstophosphoric acid (WPA), which have polyoxometalate(POM) type structure, have been shown to inhibit AChE activity in nM concentration. Circular dichroism andtryptophan fluorescence spectroscopy demonstrated that the AChE inhibition was not accompanied by significantchanges in the secondary structure of the enzyme. The molecular docking approach has revealed a newallosteric binding site, termed β-allosteric site (β-AS), which is considered responsible for the inhibition of AChEby POMs. To the best of our knowledge, this is the first study reporting a new allosteric site that is consideredresponsible for AChE inhibition by voluminous and negatively charged molecules such as POMs. The selectedPOMs were further subjected to genotoxicity testing using human peripheral blood cells as a model system. Itwas shown that WSiA and WPA induced a mild cytostatic but not genotoxic effects in human lymphocytes, whichindicates their potential to be used as medicinal drugs. The identification of non-toxic compounds capable ofbinding to an allosteric site that so far has not been considered responsible for enzyme inhibition could befundamental for the development of new drug design strategies and the discovery of more efficient AChEmodulators.
T2  - European Journal of Pharmaceutical Sciences
T1  - A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition
VL  - 151
SP  - 105376
DO  - 10.1016/j.ejps.2020.105376
ER  - 
@article{
author = "Bondžić, Aleksandra and Lazarević-Pašti, Tamara and Leskovac, Andreja and Petrović, Sandra and Čolović, Mirjana B. and Parac Vogt, Tatjana N. and Janjić, Goran V.",
year = "2020",
url = "https://vinar.vin.bg.ac.rs/handle/123456789/9028",
abstract = "Acetylcholinesterase (AChE) inhibitors are important in the treatment of neurodegenerative diseases. Two inhibitors,12-tungstosilicic acid (WSiA) and 12-tungstophosphoric acid (WPA), which have polyoxometalate(POM) type structure, have been shown to inhibit AChE activity in nM concentration. Circular dichroism andtryptophan fluorescence spectroscopy demonstrated that the AChE inhibition was not accompanied by significantchanges in the secondary structure of the enzyme. The molecular docking approach has revealed a newallosteric binding site, termed β-allosteric site (β-AS), which is considered responsible for the inhibition of AChEby POMs. To the best of our knowledge, this is the first study reporting a new allosteric site that is consideredresponsible for AChE inhibition by voluminous and negatively charged molecules such as POMs. The selectedPOMs were further subjected to genotoxicity testing using human peripheral blood cells as a model system. Itwas shown that WSiA and WPA induced a mild cytostatic but not genotoxic effects in human lymphocytes, whichindicates their potential to be used as medicinal drugs. The identification of non-toxic compounds capable ofbinding to an allosteric site that so far has not been considered responsible for enzyme inhibition could befundamental for the development of new drug design strategies and the discovery of more efficient AChEmodulators.",
journal = "European Journal of Pharmaceutical Sciences",
title = "A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition",
volume = "151",
pages = "105376",
doi = "10.1016/j.ejps.2020.105376"
}
Bondžić, A., Lazarević-Pašti, T., Leskovac, A., Petrović, S., Čolović, M. B., Parac Vogt, T. N.,& Janjić, G. V. (2020). A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition.
European Journal of Pharmaceutical Sciences, 151, 105376.
https://doi.org/10.1016/j.ejps.2020.105376
Bondžić A, Lazarević-Pašti T, Leskovac A, Petrović S, Čolović MB, Parac Vogt TN, Janjić GV. A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition. European Journal of Pharmaceutical Sciences. 2020;151:105376
Bondžić Aleksandra, Lazarević-Pašti Tamara, Leskovac Andreja, Petrović Sandra, Čolović Mirjana B., Parac Vogt Tatjana N., Janjić Goran V., "A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition" European Journal of Pharmaceutical Sciences, 151 (2020):105376,
https://doi.org/10.1016/j.ejps.2020.105376 .
4
1

UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations

Savić, Jasmina; Petrović, Sandra; Leskovac, Andreja; Lazarević-Pašti, Tamara; Nastasijević, Branislav J.; Tanović, Brankica B.; Gašić, Slavica M.; Vasić, Vesna M.

(2019)

TY  - JOUR
AU  - Savić, Jasmina
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Lazarević-Pašti, Tamara
AU  - Nastasijević, Branislav J.
AU  - Tanović, Brankica B.
AU  - Gašić, Slavica M.
AU  - Vasić, Vesna M.
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814618313670
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7821
AB  - UV-C irradiation is widely used in the food industry. However, the health effects from dietary exposure to the irradiated pesticide residues retained in foodstuffs are underestimated. In this study, technical chlorpyrifos (TCPF) and its oil in water (EW) and emulsifiable concentrate (EC) formulations were irradiated by UV-C, and their photodegradation products were subjected to toxicity assessment, including determination of acetylcholinesterase (AChE) activity, genotoxicity and oxidative stress using human blood cells as a model system. Toxicity studies were performed using the chlorpyrifos concentrations in the range of those proposed as the maximum residue levels in plant commodities. TCPF, EW and EC photodegradation products induced DNA damage and oxidative stress, and their genotoxicity did not decrease as a function of irradiation time. Irradiated TCPF and EC are more potent AChE inhibitors than irradiated EW. Accordingly, the application of UV-C irradiation must be considered when processing the plants previously treated with chlorpyrifos formulations. © 2018 Elsevier Ltd
T2  - Food Chemistry
T1  - UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations
VL  - 271
SP  - 469
EP  - 478
DO  - 10.1016/j.foodchem.2018.07.207
ER  - 
@article{
author = "Savić, Jasmina and Petrović, Sandra and Leskovac, Andreja and Lazarević-Pašti, Tamara and Nastasijević, Branislav J. and Tanović, Brankica B. and Gašić, Slavica M. and Vasić, Vesna M.",
year = "2019",
url = "https://linkinghub.elsevier.com/retrieve/pii/S0308814618313670, http://vinar.vin.bg.ac.rs/handle/123456789/7821",
abstract = "UV-C irradiation is widely used in the food industry. However, the health effects from dietary exposure to the irradiated pesticide residues retained in foodstuffs are underestimated. In this study, technical chlorpyrifos (TCPF) and its oil in water (EW) and emulsifiable concentrate (EC) formulations were irradiated by UV-C, and their photodegradation products were subjected to toxicity assessment, including determination of acetylcholinesterase (AChE) activity, genotoxicity and oxidative stress using human blood cells as a model system. Toxicity studies were performed using the chlorpyrifos concentrations in the range of those proposed as the maximum residue levels in plant commodities. TCPF, EW and EC photodegradation products induced DNA damage and oxidative stress, and their genotoxicity did not decrease as a function of irradiation time. Irradiated TCPF and EC are more potent AChE inhibitors than irradiated EW. Accordingly, the application of UV-C irradiation must be considered when processing the plants previously treated with chlorpyrifos formulations. © 2018 Elsevier Ltd",
journal = "Food Chemistry",
title = "UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations",
volume = "271",
pages = "469-478",
doi = "10.1016/j.foodchem.2018.07.207"
}
Savić, J., Petrović, S., Leskovac, A., Lazarević-Pašti, T., Nastasijević, B. J., Tanović, B. B., Gašić, S. M.,& Vasić, V. M. (2019). UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations.
Food Chemistry, 271, 469-478.
https://doi.org/10.1016/j.foodchem.2018.07.207
Savić J, Petrović S, Leskovac A, Lazarević-Pašti T, Nastasijević BJ, Tanović BB, Gašić SM, Vasić VM. UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations. Food Chemistry. 2019;271:469-478
Savić Jasmina, Petrović Sandra, Leskovac Andreja, Lazarević-Pašti Tamara, Nastasijević Branislav J., Tanović Brankica B., Gašić Slavica M., Vasić Vesna M., "UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations" Food Chemistry, 271 (2019):469-478,
https://doi.org/10.1016/j.foodchem.2018.07.207 .
8
7

Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations

Savić, Jasmina; Petrović, Sandra; Leskovac, Andreja; Lazarević-Pašti, Tamara; Nastasijević, Branislav J.; Tanović, Brankica B.; Gašić, Slavica M.; Vasić, Vesna M.

(2019)

TY  - BOOK
AU  - Savić, Jasmina
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Lazarević-Pašti, Tamara
AU  - Nastasijević, Branislav J.
AU  - Tanović, Brankica B.
AU  - Gašić, Slavica M.
AU  - Vasić, Vesna M.
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814618313670
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7831
AB  - Supplementary data 1: Table 1S. The chromatographic gradient profile; Table 2S. CPF concentration decrease (corresponding initial CPF concentration decrease was set as 0%) for all three forms of CPF depending on irradiation time; Table 3S. CPF and CPO concentrations determined chromatographically for TCPF, EW and EC formulations, as the function of irradiation time; % of CPO comparing to initial CPF concentration in all three forms of CPF; 
Supplementary data 2: Material safety data sheet according to 1907/2006/EC, Article 31/version 1; 
Supplementary data 3: Material safety data sheet according to 1907/2006/EC, Article 31/version 4
T2  - Food Chemistry
T1  - Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations
VL  - 271
SP  - 469
EP  - 478
DO  - 10.1016/j.foodchem.2018.07.207
ER  - 
@book{
author = "Savić, Jasmina and Petrović, Sandra and Leskovac, Andreja and Lazarević-Pašti, Tamara and Nastasijević, Branislav J. and Tanović, Brankica B. and Gašić, Slavica M. and Vasić, Vesna M.",
year = "2019",
url = "https://linkinghub.elsevier.com/retrieve/pii/S0308814618313670, http://vinar.vin.bg.ac.rs/handle/123456789/7831",
abstract = "Supplementary data 1: Table 1S. The chromatographic gradient profile; Table 2S. CPF concentration decrease (corresponding initial CPF concentration decrease was set as 0%) for all three forms of CPF depending on irradiation time; Table 3S. CPF and CPO concentrations determined chromatographically for TCPF, EW and EC formulations, as the function of irradiation time; % of CPO comparing to initial CPF concentration in all three forms of CPF; 
Supplementary data 2: Material safety data sheet according to 1907/2006/EC, Article 31/version 1; 
Supplementary data 3: Material safety data sheet according to 1907/2006/EC, Article 31/version 4",
journal = "Food Chemistry",
title = "Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations",
volume = "271",
pages = "469-478",
doi = "10.1016/j.foodchem.2018.07.207"
}
Savić, J., Petrović, S., Leskovac, A., Lazarević-Pašti, T., Nastasijević, B. J., Tanović, B. B., Gašić, S. M.,& Vasić, V. M. (2019). Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations.
Food Chemistry, 271, 469-478.
https://doi.org/10.1016/j.foodchem.2018.07.207
Savić J, Petrović S, Leskovac A, Lazarević-Pašti T, Nastasijević BJ, Tanović BB, Gašić SM, Vasić VM. Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations. Food Chemistry. 2019;271:469-478
Savić Jasmina, Petrović Sandra, Leskovac Andreja, Lazarević-Pašti Tamara, Nastasijević Branislav J., Tanović Brankica B., Gašić Slavica M., Vasić Vesna M., "Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations" Food Chemistry, 271 (2019):469-478,
https://doi.org/10.1016/j.foodchem.2018.07.207 .
8
7

Non-thermal plasma needle as an effective tool in dimethoate removal from water

Mitrović, Tatjana; Lazović, Saša; Nastasijević, Branislav J.; Pašti, Igor A.; Vasić, Vesna M.; Lazarević-Pašti, Tamara

(2019)

TY  - JOUR
AU  - Mitrović, Tatjana
AU  - Lazović, Saša
AU  - Nastasijević, Branislav J.
AU  - Pašti, Igor A.
AU  - Vasić, Vesna M.
AU  - Lazarević-Pašti, Tamara
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0301479719307716
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8337
AB  - Intensive use of pesticides requires innovative approaches for their removal from the environment. Here we report the method for degradation of dimethoate in water using non-thermal plasma needle and analyze kinetics of dimethoate removal and possible degradation pathways. The effects of dimethoate initial concentration, plasma treatment time, Argon flow rate and the presence of radical promoters on the effectiveness of proposed method are evaluated. With argon flow rate of 0.5 slm (standard litres per minute) 1 × 10−4 M dimethoate can be removed within 30 min of treatment. Using UPLC analysis it was confirmed that one of the decomposition products is dimethoate oxo-analogue omethoate, which is in fact more toxic than dimethoate. However, the overall toxicity of contaminated water was reduced upon the treatment. The addition of H2O2 as a free radical promoter enhances dimethoate removal, while K2S2O8 results with selective conversion to omethoate. Using mass spectrometry in combination with the theoretical calculations, possible degradation pathways were proposed. The feasibility of the proposed method for dimethoate degradation in real water samples is confirmed. The proposed method is demonstrated as a highly effective approach for dimethoate removal without significant accumulation of undesirable toxic products and secondary waste. © 2019 Elsevier Ltd
T2  - Journal of Environmental Management
T1  - Non-thermal plasma needle as an effective tool in dimethoate removal from water
VL  - 246
SP  - 63
EP  - 70
DO  - 10.1016/j.jenvman.2019.05.143
ER  - 
@article{
author = "Mitrović, Tatjana and Lazović, Saša and Nastasijević, Branislav J. and Pašti, Igor A. and Vasić, Vesna M. and Lazarević-Pašti, Tamara",
year = "2019",
url = "https://linkinghub.elsevier.com/retrieve/pii/S0301479719307716, http://vinar.vin.bg.ac.rs/handle/123456789/8337",
abstract = "Intensive use of pesticides requires innovative approaches for their removal from the environment. Here we report the method for degradation of dimethoate in water using non-thermal plasma needle and analyze kinetics of dimethoate removal and possible degradation pathways. The effects of dimethoate initial concentration, plasma treatment time, Argon flow rate and the presence of radical promoters on the effectiveness of proposed method are evaluated. With argon flow rate of 0.5 slm (standard litres per minute) 1 × 10−4 M dimethoate can be removed within 30 min of treatment. Using UPLC analysis it was confirmed that one of the decomposition products is dimethoate oxo-analogue omethoate, which is in fact more toxic than dimethoate. However, the overall toxicity of contaminated water was reduced upon the treatment. The addition of H2O2 as a free radical promoter enhances dimethoate removal, while K2S2O8 results with selective conversion to omethoate. Using mass spectrometry in combination with the theoretical calculations, possible degradation pathways were proposed. The feasibility of the proposed method for dimethoate degradation in real water samples is confirmed. The proposed method is demonstrated as a highly effective approach for dimethoate removal without significant accumulation of undesirable toxic products and secondary waste. © 2019 Elsevier Ltd",
journal = "Journal of Environmental Management",
title = "Non-thermal plasma needle as an effective tool in dimethoate removal from water",
volume = "246",
pages = "63-70",
doi = "10.1016/j.jenvman.2019.05.143"
}
Mitrović, T., Lazović, S., Nastasijević, B. J., Pašti, I. A., Vasić, V. M.,& Lazarević-Pašti, T. (2019). Non-thermal plasma needle as an effective tool in dimethoate removal from water.
Journal of Environmental Management, 246, 63-70.
https://doi.org/10.1016/j.jenvman.2019.05.143
Mitrović T, Lazović S, Nastasijević BJ, Pašti IA, Vasić VM, Lazarević-Pašti T. Non-thermal plasma needle as an effective tool in dimethoate removal from water. Journal of Environmental Management. 2019;246:63-70
Mitrović Tatjana, Lazović Saša, Nastasijević Branislav J., Pašti Igor A., Vasić Vesna M., Lazarević-Pašti Tamara, "Non-thermal plasma needle as an effective tool in dimethoate removal from water" Journal of Environmental Management, 246 (2019):63-70,
https://doi.org/10.1016/j.jenvman.2019.05.143 .
7
5
8

Detection of Dimethoate Pesticide using Layer by Layer Deposition of PDAC/GO on Ag electrode

Ega, Tharun K; Al-Hamry, Ammar; Kanoun, Olfa; Lazarević-Pašti, Tamara; Bogdanović, Danica B.; Pašti, Igor A.; Rodriguez, Raul D.; Sheremet, Evgeniya; Paterno, Leonardo G.

(IEEE, 2019)

TY  - CONF
AU  - Ega, Tharun K
AU  - Al-Hamry, Ammar
AU  - Kanoun, Olfa
AU  - Lazarević-Pašti, Tamara
AU  - Bogdanović, Danica B.
AU  - Pašti, Igor A.
AU  - Rodriguez, Raul D.
AU  - Sheremet, Evgeniya
AU  - Paterno, Leonardo G.
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8665
AB  - Dimethoate (DMT) is an organophosphate pesticide (OP), which is widely used against insects and mites and their control in agriculture. As other OPs, DMT is also an inhibitor of acetylcholinesterase, which is responsible for the disabling of cholinesterase required for the functioning of the central nervous system. This pesticide can invade living cells of the human body through contact or ingestion. We report an electrochemical sensor based on a layer by layer deposition of PDAC/GO on silver electrodes. The sensor fabrication, physical characterization i.e. Raman spectroscopy and scanning electron microscopy of PDAC/GO based films, and its electrochemical characterization are discussed. The detection of DMT by analyzing electrochemical measurements including cyclic voltammetry and impedance spectroscopy shows that functionalization using layer by layer deposition improves electrochemical response and presents a basis for detection of DMT. The highest response is observed in the case of only one PDAC/GO layer which is attributed to the properly balanced interaction between DMT and PDAC/GO layer, and the increase of electrical resistivity of the PDAC/GO layer with its thickness. © 2019 IEEE.
PB  - IEEE
C3  - 2019 16th International Multi-Conference on Systems, Signals & Devices (SSD)
C3  - 2019 16th International Multi-Conference on Systems, Signals & Devices (SSD)
T1  - Detection of Dimethoate Pesticide using Layer by Layer Deposition of PDAC/GO on Ag electrode
SP  - 621
EP  - 625
DO  - 10.1109/SSD.2019.8893253
ER  - 
@conference{
author = "Ega, Tharun K and Al-Hamry, Ammar and Kanoun, Olfa and Lazarević-Pašti, Tamara and Bogdanović, Danica B. and Pašti, Igor A. and Rodriguez, Raul D. and Sheremet, Evgeniya and Paterno, Leonardo G.",
year = "2019",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8665",
abstract = "Dimethoate (DMT) is an organophosphate pesticide (OP), which is widely used against insects and mites and their control in agriculture. As other OPs, DMT is also an inhibitor of acetylcholinesterase, which is responsible for the disabling of cholinesterase required for the functioning of the central nervous system. This pesticide can invade living cells of the human body through contact or ingestion. We report an electrochemical sensor based on a layer by layer deposition of PDAC/GO on silver electrodes. The sensor fabrication, physical characterization i.e. Raman spectroscopy and scanning electron microscopy of PDAC/GO based films, and its electrochemical characterization are discussed. The detection of DMT by analyzing electrochemical measurements including cyclic voltammetry and impedance spectroscopy shows that functionalization using layer by layer deposition improves electrochemical response and presents a basis for detection of DMT. The highest response is observed in the case of only one PDAC/GO layer which is attributed to the properly balanced interaction between DMT and PDAC/GO layer, and the increase of electrical resistivity of the PDAC/GO layer with its thickness. © 2019 IEEE.",
publisher = "IEEE",
journal = "2019 16th International Multi-Conference on Systems, Signals & Devices (SSD), 2019 16th International Multi-Conference on Systems, Signals & Devices (SSD)",
title = "Detection of Dimethoate Pesticide using Layer by Layer Deposition of PDAC/GO on Ag electrode",
pages = "621-625",
doi = "10.1109/SSD.2019.8893253"
}
Ega, T. K., Al-Hamry, A., Kanoun, O., Lazarević-Pašti, T., Bogdanović, D. B., Pašti, I. A., Rodriguez, R. D., Sheremet, E.,& Paterno, L. G. (2019). Detection of Dimethoate Pesticide using Layer by Layer Deposition of PDAC/GO on Ag electrode.
2019 16th International Multi-Conference on Systems, Signals & Devices (SSD)
IEEE., 621-625.
https://doi.org/10.1109/SSD.2019.8893253
Ega TK, Al-Hamry A, Kanoun O, Lazarević-Pašti T, Bogdanović DB, Pašti IA, Rodriguez RD, Sheremet E, Paterno LG. Detection of Dimethoate Pesticide using Layer by Layer Deposition of PDAC/GO on Ag electrode. 2019 16th International Multi-Conference on Systems, Signals & Devices (SSD). 2019;:621-625
Ega Tharun K, Al-Hamry Ammar, Kanoun Olfa, Lazarević-Pašti Tamara, Bogdanović Danica B., Pašti Igor A., Rodriguez Raul D., Sheremet Evgeniya, Paterno Leonardo G., "Detection of Dimethoate Pesticide using Layer by Layer Deposition of PDAC/GO on Ag electrode" 2019 16th International Multi-Conference on Systems, Signals & Devices (SSD) (2019):621-625,
https://doi.org/10.1109/SSD.2019.8893253 .
3
2

The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water

Lazarević-Pašti, Tamara; Anićijević, Vladan J.; Baljozović, Miloš; Vasić Anićijević, Dragana D.; Gutić, Sanjin J.; Vasić, Vesna M.; Skorodumova, Natalia V.; Pašti, Igor A.

(2018)

TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Anićijević, Vladan J.
AU  - Baljozović, Miloš
AU  - Vasić Anićijević, Dragana D.
AU  - Gutić, Sanjin J.
AU  - Vasić, Vesna M.
AU  - Skorodumova, Natalia V.
AU  - Pašti, Igor A.
PY  - 2018
UR  - http://xlink.rsc.org/?DOI=C8EN00171E
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7730
AB  - The wide use of pesticides in modern agriculture and other areas results in an urgent need for their efficient removal from the environment. Adsorption of pesticides is one of the most commonly used strategies for this task. Here we analyze the adsorption of two organophosphorus pesticides, dimethoate (DMT) and chlorpyrifos (CPF), on graphene-based materials. The adsorption was found to be very sensitive to the structure of the adsorbents used. In particular, aliphatic DMT was found to prefer hydrophilic oxidized graphene surfaces. The CPF molecule, which contains an aromatic moiety, prefers adsorption on the surface of a graphene basal plane with high structural order and preserved π electron system. The toxicity of pesticide solutions is reduced after adsorption, suggesting that there is no oxidation of DMT and CPF to more toxic oxo forms. We emphasize that the combination of structural properties of adsorbents and adsorbates defines the adsorption of organophosphorus pesticides on graphene-based materials, while the specific surface area of adsorbents is not the major factor.
T2  - Environmental Science: Nano
T1  - The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water
VL  - 5
IS  - 6
SP  - 1482
EP  - 1494
DO  - 10.1039/C8EN00171E
ER  - 
@article{
author = "Lazarević-Pašti, Tamara and Anićijević, Vladan J. and Baljozović, Miloš and Vasić Anićijević, Dragana D. and Gutić, Sanjin J. and Vasić, Vesna M. and Skorodumova, Natalia V. and Pašti, Igor A.",
year = "2018",
url = "http://xlink.rsc.org/?DOI=C8EN00171E, http://vinar.vin.bg.ac.rs/handle/123456789/7730",
abstract = "The wide use of pesticides in modern agriculture and other areas results in an urgent need for their efficient removal from the environment. Adsorption of pesticides is one of the most commonly used strategies for this task. Here we analyze the adsorption of two organophosphorus pesticides, dimethoate (DMT) and chlorpyrifos (CPF), on graphene-based materials. The adsorption was found to be very sensitive to the structure of the adsorbents used. In particular, aliphatic DMT was found to prefer hydrophilic oxidized graphene surfaces. The CPF molecule, which contains an aromatic moiety, prefers adsorption on the surface of a graphene basal plane with high structural order and preserved π electron system. The toxicity of pesticide solutions is reduced after adsorption, suggesting that there is no oxidation of DMT and CPF to more toxic oxo forms. We emphasize that the combination of structural properties of adsorbents and adsorbates defines the adsorption of organophosphorus pesticides on graphene-based materials, while the specific surface area of adsorbents is not the major factor.",
journal = "Environmental Science: Nano",
title = "The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water",
volume = "5",
number = "6",
pages = "1482-1494",
doi = "10.1039/C8EN00171E"
}
Lazarević-Pašti, T., Anićijević, V. J., Baljozović, M., Vasić Anićijević, D. D., Gutić, S. J., Vasić, V. M., Skorodumova, N. V.,& Pašti, I. A. (2018). The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water.
Environmental Science: Nano, 5(6), 1482-1494.
https://doi.org/10.1039/C8EN00171E
Lazarević-Pašti T, Anićijević VJ, Baljozović M, Vasić Anićijević DD, Gutić SJ, Vasić VM, Skorodumova NV, Pašti IA. The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water. Environmental Science: Nano. 2018;5(6):1482-1494
Lazarević-Pašti Tamara, Anićijević Vladan J., Baljozović Miloš, Vasić Anićijević Dragana D., Gutić Sanjin J., Vasić Vesna M., Skorodumova Natalia V., Pašti Igor A., "The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water" Environmental Science: Nano, 5, no. 6 (2018):1482-1494,
https://doi.org/10.1039/C8EN00171E .
7
29
15
28

Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents

Leskovac, Andreja; Petrović, Sandra; Lazarević-Pašti, Tamara; Krstić, Milena P.; Vasić, Vesna M.

(2018)

TY  - JOUR
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Lazarević-Pašti, Tamara
AU  - Krstić, Milena P.
AU  - Vasić, Vesna M.
PY  - 2018
UR  - http://link.springer.com/10.1007/s00775-018-1560-x
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7765
AB  - In recent years, the search for effective anticancer compounds based on transition metal complexes has been the focus of medical investigations. The synergy between the ruthenium(II) and N-alkylphenothiazine counter-ions (chlorpromazine hydrochloride, thioridazine hydrochloride and trifluoperazine dihydrochloride, respectively) through the formation of three different complexes (1–3) was investigated. We explored whether the selected counter-ions and complexes might affect redox homeostasis and genome integrity of normal human blood cells, and induce an inhibition of Na+/K+-ATPase and AChE at pharmacologically relevant doses. Our results have shown that counter-ions and complexes did not affect the activity of Na+/K+-ATPase, while AChE activity was inhibited in a dose-dependent manner. All investigated compounds disturbed the viability and redox homeostasis of lymphocytes. Complexes 1 and 2 displayed potent cytotoxic and prooxidant action while complex 3 behaved as a weaker genotoxic inducer. Still, the tested complexes appeared to be less genotoxic and more cytostatic than the corresponding counter-ions. The effects of selected complexes were also tested in PC12 and U2OS cancer cells with special attention being given to the ability of phenothiazines to affect dopamine D2 receptors. Using the confocal laser scanning microscopy, we observed that all the complexes reduced cell viability. Although all investigated complexes have been bound to the dopamine receptor D2-eGFP, only complex 3 reduced its surface density and increased its lateral mobility in investigated cell lines. Albeit the role of alternative targets for complex 3 cannot be ruled out, its effects should be further examined as potential treatment strategy against cancer cells that overexpress D2.
T2  - JBIC Journal of Biological Inorganic Chemistry
T1  - Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents
VL  - 23
IS  - 5
SP  - 689
EP  - 704
DO  - 10.1007/s00775-018-1560-x
ER  - 
@article{
author = "Leskovac, Andreja and Petrović, Sandra and Lazarević-Pašti, Tamara and Krstić, Milena P. and Vasić, Vesna M.",
year = "2018",
url = "http://link.springer.com/10.1007/s00775-018-1560-x, http://vinar.vin.bg.ac.rs/handle/123456789/7765",
abstract = "In recent years, the search for effective anticancer compounds based on transition metal complexes has been the focus of medical investigations. The synergy between the ruthenium(II) and N-alkylphenothiazine counter-ions (chlorpromazine hydrochloride, thioridazine hydrochloride and trifluoperazine dihydrochloride, respectively) through the formation of three different complexes (1–3) was investigated. We explored whether the selected counter-ions and complexes might affect redox homeostasis and genome integrity of normal human blood cells, and induce an inhibition of Na+/K+-ATPase and AChE at pharmacologically relevant doses. Our results have shown that counter-ions and complexes did not affect the activity of Na+/K+-ATPase, while AChE activity was inhibited in a dose-dependent manner. All investigated compounds disturbed the viability and redox homeostasis of lymphocytes. Complexes 1 and 2 displayed potent cytotoxic and prooxidant action while complex 3 behaved as a weaker genotoxic inducer. Still, the tested complexes appeared to be less genotoxic and more cytostatic than the corresponding counter-ions. The effects of selected complexes were also tested in PC12 and U2OS cancer cells with special attention being given to the ability of phenothiazines to affect dopamine D2 receptors. Using the confocal laser scanning microscopy, we observed that all the complexes reduced cell viability. Although all investigated complexes have been bound to the dopamine receptor D2-eGFP, only complex 3 reduced its surface density and increased its lateral mobility in investigated cell lines. Albeit the role of alternative targets for complex 3 cannot be ruled out, its effects should be further examined as potential treatment strategy against cancer cells that overexpress D2.",
journal = "JBIC Journal of Biological Inorganic Chemistry",
title = "Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents",
volume = "23",
number = "5",
pages = "689-704",
doi = "10.1007/s00775-018-1560-x"
}
Leskovac, A., Petrović, S., Lazarević-Pašti, T., Krstić, M. P.,& Vasić, V. M. (2018). Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents.
JBIC Journal of Biological Inorganic Chemistry, 23(5), 689-704.
https://doi.org/10.1007/s00775-018-1560-x
Leskovac A, Petrović S, Lazarević-Pašti T, Krstić MP, Vasić VM. Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents. JBIC Journal of Biological Inorganic Chemistry. 2018;23(5):689-704
Leskovac Andreja, Petrović Sandra, Lazarević-Pašti Tamara, Krstić Milena P., Vasić Vesna M., "Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents" JBIC Journal of Biological Inorganic Chemistry, 23, no. 5 (2018):689-704,
https://doi.org/10.1007/s00775-018-1560-x .
1
1

Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs

Lazarević-Pašti, Tamara; Leskovac, Andreja; Momić, Tatjana; Petrović, Sandra; Vasić, Vesna M.

(2017)

TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Leskovac, Andreja
AU  - Momić, Tatjana
AU  - Petrović, Sandra
AU  - Vasić, Vesna M.
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1796
AB  - Background: Acetylcholinesterase (AChE) is involved in the termination of impulse transmission by rapid hydrolysis of the neurotransmitter acetylcholine in numerous cholinergic pathways in the central and peripheral nervous systems. The enzyme inactivation leads to acetylcholine accumulation, hyperstimulation of nicotinic and muscarinic receptors, and disrupted neurotransmission. Hence, acetylcholinesterase inhibitors, interacting with the enzyme as their primary target, are applied as relevant drugs for different neurodegenerative diseases (such as Alzheimers and Parkinsons) as well as toxins. At the same time, there are increasing evidence that in non-neuronal context, AChE is involved in the regulation of cell proliferation, differentiation, apoptosis and cell-cell interaction. An irregular expression of AChE has been found in different types of tumors, suggesting the involvement of AChE in the regulation of tumor development. Having all this in mind, there is a possibility that some AChE inhibitors could be used as anti-cancer agents. Objective: This contribution will discuss a broad range of possible application of different AChE inhibitors as drugs, from well-known anti-Alzheimers disease drugs to their use in cancer treatment in future. Emphasis will be put on various known AChE inhibitors classes, whose application as drugs could be controversy, as well as on newly investigated natural products, which can also modulate AChE activity. Conclusion: It is not clear a patient treated for neurodegenerative condition prone to increased risk for some types of cancer and vice versa. This is necessary to keep in mind during rational drug design process for all therapies, which are based on AChE as a target molecule.
T2  - Current Medicinal Chemistry
T1  - Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs
VL  - 24
IS  - 30
SP  - 3283
EP  - 3309
DO  - 10.2174/0929867324666170705123509
ER  - 
@article{
author = "Lazarević-Pašti, Tamara and Leskovac, Andreja and Momić, Tatjana and Petrović, Sandra and Vasić, Vesna M.",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1796",
abstract = "Background: Acetylcholinesterase (AChE) is involved in the termination of impulse transmission by rapid hydrolysis of the neurotransmitter acetylcholine in numerous cholinergic pathways in the central and peripheral nervous systems. The enzyme inactivation leads to acetylcholine accumulation, hyperstimulation of nicotinic and muscarinic receptors, and disrupted neurotransmission. Hence, acetylcholinesterase inhibitors, interacting with the enzyme as their primary target, are applied as relevant drugs for different neurodegenerative diseases (such as Alzheimers and Parkinsons) as well as toxins. At the same time, there are increasing evidence that in non-neuronal context, AChE is involved in the regulation of cell proliferation, differentiation, apoptosis and cell-cell interaction. An irregular expression of AChE has been found in different types of tumors, suggesting the involvement of AChE in the regulation of tumor development. Having all this in mind, there is a possibility that some AChE inhibitors could be used as anti-cancer agents. Objective: This contribution will discuss a broad range of possible application of different AChE inhibitors as drugs, from well-known anti-Alzheimers disease drugs to their use in cancer treatment in future. Emphasis will be put on various known AChE inhibitors classes, whose application as drugs could be controversy, as well as on newly investigated natural products, which can also modulate AChE activity. Conclusion: It is not clear a patient treated for neurodegenerative condition prone to increased risk for some types of cancer and vice versa. This is necessary to keep in mind during rational drug design process for all therapies, which are based on AChE as a target molecule.",
journal = "Current Medicinal Chemistry",
title = "Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs",
volume = "24",
number = "30",
pages = "3283-3309",
doi = "10.2174/0929867324666170705123509"
}
Lazarević-Pašti, T., Leskovac, A., Momić, T., Petrović, S.,& Vasić, V. M. (2017). Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs.
Current Medicinal Chemistry, 24(30), 3283-3309.
https://doi.org/10.2174/0929867324666170705123509
Lazarević-Pašti T, Leskovac A, Momić T, Petrović S, Vasić VM. Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs. Current Medicinal Chemistry. 2017;24(30):3283-3309
Lazarević-Pašti Tamara, Leskovac Andreja, Momić Tatjana, Petrović Sandra, Vasić Vesna M., "Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs" Current Medicinal Chemistry, 24, no. 30 (2017):3283-3309,
https://doi.org/10.2174/0929867324666170705123509 .
7
35
31
36

Heteroatom-doped mesoporous carbons as efficient adsorbents for removal of dimethoate and omethoate from water

Lazarević-Pašti, Tamara; Pašti, Igor A.; Jokić, Bojan M.; Babić, Biljana M.; Vasić, Vesna M.

(2016)

TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Pašti, Igor A.
AU  - Jokić, Bojan M.
AU  - Babić, Biljana M.
AU  - Vasić, Vesna M.
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1178
AB  - Extensive use of organophosphate pesticides (OPs) invokes development of efficient procedures for their removal from the environment. By introducing low levels ( LT 1 at%) of B, N or P into the structure of mesoporous carbons, we have produced a series of materials with different surface chemical composition, textural properties and level of structural disorder. These adsorbents were applied for removal of dimethoate and its oxo-analogue omethoate from aqueous solutions under batch adsorption conditions and by filtration using modified nylon membrane filters. Adsorption capacities up to 164 mg g(-1) were measured, with OPs uptake typically above 80% for dimethoate concentration as high as 5 x 10(-3) mol dm(-3). After the adsorption, neurotoxic effects of OP-containing water samples were significantly reduced or completely removed. The level of structural disorder was identified as a key parameter for efficient removal of dimethoate and omethoate while in the filtration experiments surface area of adsorbents also played an important role. While the presented research appeals to new fundamental studies of OP-carbon surface interactions, it also indicates a possible strategy in designing new efficient adsorbents for OPs removal from water.
T2  - RSC Advances
T1  - Heteroatom-doped mesoporous carbons as efficient adsorbents for removal of dimethoate and omethoate from water
VL  - 6
IS  - 67
SP  - 62128
EP  - 62139
DO  - 10.1039/c6ra06736k
ER  - 
@article{
author = "Lazarević-Pašti, Tamara and Pašti, Igor A. and Jokić, Bojan M. and Babić, Biljana M. and Vasić, Vesna M.",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1178",
abstract = "Extensive use of organophosphate pesticides (OPs) invokes development of efficient procedures for their removal from the environment. By introducing low levels ( LT 1 at%) of B, N or P into the structure of mesoporous carbons, we have produced a series of materials with different surface chemical composition, textural properties and level of structural disorder. These adsorbents were applied for removal of dimethoate and its oxo-analogue omethoate from aqueous solutions under batch adsorption conditions and by filtration using modified nylon membrane filters. Adsorption capacities up to 164 mg g(-1) were measured, with OPs uptake typically above 80% for dimethoate concentration as high as 5 x 10(-3) mol dm(-3). After the adsorption, neurotoxic effects of OP-containing water samples were significantly reduced or completely removed. The level of structural disorder was identified as a key parameter for efficient removal of dimethoate and omethoate while in the filtration experiments surface area of adsorbents also played an important role. While the presented research appeals to new fundamental studies of OP-carbon surface interactions, it also indicates a possible strategy in designing new efficient adsorbents for OPs removal from water.",
journal = "RSC Advances",
title = "Heteroatom-doped mesoporous carbons as efficient adsorbents for removal of dimethoate and omethoate from water",
volume = "6",
number = "67",
pages = "62128-62139",
doi = "10.1039/c6ra06736k"
}
Lazarević-Pašti, T., Pašti, I. A., Jokić, B. M., Babić, B. M.,& Vasić, V. M. (2016). Heteroatom-doped mesoporous carbons as efficient adsorbents for removal of dimethoate and omethoate from water.
RSC Advances, 6(67), 62128-62139.
https://doi.org/10.1039/c6ra06736k
Lazarević-Pašti T, Pašti IA, Jokić BM, Babić BM, Vasić VM. Heteroatom-doped mesoporous carbons as efficient adsorbents for removal of dimethoate and omethoate from water. RSC Advances. 2016;6(67):62128-62139
Lazarević-Pašti Tamara, Pašti Igor A., Jokić Bojan M., Babić Biljana M., Vasić Vesna M., "Heteroatom-doped mesoporous carbons as efficient adsorbents for removal of dimethoate and omethoate from water" RSC Advances, 6, no. 67 (2016):62128-62139,
https://doi.org/10.1039/c6ra06736k .
14
10
13

Adsorption of malathion on mesoporous monetite obtained by mechanochemical treatment of brushite

Mirković, Miljana M.; Lazarević-Pašti, Tamara; Došen, Anja M.; Čebela, Maria; Rosic, A. A.; Matović, Branko; Babić, Biljana M.

(2016)

TY  - JOUR
AU  - Mirković, Miljana M.
AU  - Lazarević-Pašti, Tamara
AU  - Došen, Anja M.
AU  - Čebela, Maria
AU  - Rosic, A. A.
AU  - Matović, Branko
AU  - Babić, Biljana M.
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/930
AB  - Mesoporous monetite (CaHPO4), obtained by mechanochemical treatment of previously synthesized brushite (CaHPO4 center dot 2H(2)O), was used as efficient adsorbent for the organic pesticide malathion. The structure of brushite was confirmed by Raman spectroscopy. The phase transformation process was investigated by X-ray powder diffraction (XRD) and Fourier transformation infra-red spectroscopy (FTIR). The microstructure and morphology were determined by scanning electron microscopy (SEM) and the nitrogen adsorption-desorption method. It was found that five minutes of milling induces brushite-monetite phase transformation. Adsorption of malathion from aqueous solutions showed that this pesticide can be successfully adsorbed on surface of this material.
T2  - RSC Advances
T1  - Adsorption of malathion on mesoporous monetite obtained by mechanochemical treatment of brushite
VL  - 6
IS  - 15
SP  - 12219
EP  - 12225
DO  - 10.1039/c5ra27554g
ER  - 
@article{
author = "Mirković, Miljana M. and Lazarević-Pašti, Tamara and Došen, Anja M. and Čebela, Maria and Rosic, A. A. and Matović, Branko and Babić, Biljana M.",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/930",
abstract = "Mesoporous monetite (CaHPO4), obtained by mechanochemical treatment of previously synthesized brushite (CaHPO4 center dot 2H(2)O), was used as efficient adsorbent for the organic pesticide malathion. The structure of brushite was confirmed by Raman spectroscopy. The phase transformation process was investigated by X-ray powder diffraction (XRD) and Fourier transformation infra-red spectroscopy (FTIR). The microstructure and morphology were determined by scanning electron microscopy (SEM) and the nitrogen adsorption-desorption method. It was found that five minutes of milling induces brushite-monetite phase transformation. Adsorption of malathion from aqueous solutions showed that this pesticide can be successfully adsorbed on surface of this material.",
journal = "RSC Advances",
title = "Adsorption of malathion on mesoporous monetite obtained by mechanochemical treatment of brushite",
volume = "6",
number = "15",
pages = "12219-12225",
doi = "10.1039/c5ra27554g"
}
Mirković, M. M., Lazarević-Pašti, T., Došen, A. M., Čebela, M., Rosic, A. A., Matović, B.,& Babić, B. M. (2016). Adsorption of malathion on mesoporous monetite obtained by mechanochemical treatment of brushite.
RSC Advances, 6(15), 12219-12225.
https://doi.org/10.1039/c5ra27554g
Mirković MM, Lazarević-Pašti T, Došen AM, Čebela M, Rosic AA, Matović B, Babić BM. Adsorption of malathion on mesoporous monetite obtained by mechanochemical treatment of brushite. RSC Advances. 2016;6(15):12219-12225
Mirković Miljana M., Lazarević-Pašti Tamara, Došen Anja M., Čebela Maria, Rosic A. A., Matović Branko, Babić Biljana M., "Adsorption of malathion on mesoporous monetite obtained by mechanochemical treatment of brushite" RSC Advances, 6, no. 15 (2016):12219-12225,
https://doi.org/10.1039/c5ra27554g .
27
24
30

Adsorption of Organophosphate Pesticide Dimethoate on Gold Nanospheres and Nanorods

Momić, Tatjana; Lazarević-Pašti, Tamara; Bogdanović, Una; Vodnik, Vesna; Mraković, Ana Đ.; Rakočević, Zlatko Lj.; Pavlović, Vladimir B.; Vasić, Vesna M.

(2016)

TY  - JOUR
AU  - Momić, Tatjana
AU  - Lazarević-Pašti, Tamara
AU  - Bogdanović, Una
AU  - Vodnik, Vesna
AU  - Mraković, Ana Đ.
AU  - Rakočević, Zlatko Lj.
AU  - Pavlović, Vladimir B.
AU  - Vasić, Vesna M.
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1347
AB  - Organophosphorus pesticide dimethoate was adsorbed onto gold nanospheres and nanorods in aqueous solution using batch technique. Adsorption of dimethoate onto gold nanoparticles was confirmed by UV-Vis spectrophotometry, TEM, AFM, and FTIR analysis. The adsorption of nanospheres resulted in aggregation which was not the case with nanorods. Nanoparticles adsorption features were characterized using Langmuir and Freundlich isotherm models. The Langmuir adsorption isotherm was found to have the best fit to the experimental data for both types of nanoparticles. Adsorption capacity detected for nanospheres is 456 mg/g and for nanorods is 57.1 mg/g. Also, nanoparticles were successfully used for dimethoate removal from spiked drinking water while nanospheres were shown to be more efficient than nanorods.
T2  - Journal of Nanomaterials
T1  - Adsorption of Organophosphate Pesticide Dimethoate on Gold Nanospheres and Nanorods
DO  - 10.1155/2016/8910271
ER  - 
@article{
author = "Momić, Tatjana and Lazarević-Pašti, Tamara and Bogdanović, Una and Vodnik, Vesna and Mraković, Ana Đ. and Rakočević, Zlatko Lj. and Pavlović, Vladimir B. and Vasić, Vesna M.",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1347",
abstract = "Organophosphorus pesticide dimethoate was adsorbed onto gold nanospheres and nanorods in aqueous solution using batch technique. Adsorption of dimethoate onto gold nanoparticles was confirmed by UV-Vis spectrophotometry, TEM, AFM, and FTIR analysis. The adsorption of nanospheres resulted in aggregation which was not the case with nanorods. Nanoparticles adsorption features were characterized using Langmuir and Freundlich isotherm models. The Langmuir adsorption isotherm was found to have the best fit to the experimental data for both types of nanoparticles. Adsorption capacity detected for nanospheres is 456 mg/g and for nanorods is 57.1 mg/g. Also, nanoparticles were successfully used for dimethoate removal from spiked drinking water while nanospheres were shown to be more efficient than nanorods.",
journal = "Journal of Nanomaterials",
title = "Adsorption of Organophosphate Pesticide Dimethoate on Gold Nanospheres and Nanorods",
doi = "10.1155/2016/8910271"
}
Momić, T., Lazarević-Pašti, T., Bogdanović, U., Vodnik, V., Mraković, A. Đ., Rakočević, Z. Lj., Pavlović, V. B.,& Vasić, V. M. (2016). Adsorption of Organophosphate Pesticide Dimethoate on Gold Nanospheres and Nanorods.
Journal of Nanomaterials.
https://doi.org/10.1155/2016/8910271
Momić T, Lazarević-Pašti T, Bogdanović U, Vodnik V, Mraković AĐ, Rakočević ZL, Pavlović VB, Vasić VM. Adsorption of Organophosphate Pesticide Dimethoate on Gold Nanospheres and Nanorods. Journal of Nanomaterials. 2016;
Momić Tatjana, Lazarević-Pašti Tamara, Bogdanović Una, Vodnik Vesna, Mraković Ana Đ., Rakočević Zlatko Lj., Pavlović Vladimir B., Vasić Vesna M., "Adsorption of Organophosphate Pesticide Dimethoate on Gold Nanospheres and Nanorods" Journal of Nanomaterials (2016),
https://doi.org/10.1155/2016/8910271 .
23
12
14

Myeloperoxidase Inhibitors as Potential Drugs

Lazarević-Pašti, Tamara; Leskovac, Andreja; Vasić, Vesna M.

(2015)

TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Leskovac, Andreja
AU  - Vasić, Vesna M.
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/700
AB  - Myeloperoxidase (MPO) is an important member of the haem peroxidase - cyclooxygenase superfamily. This enzyme is physiologically expressed in circulating neutrophils, monocytes and some tissue macrophages including microglia. MPO plays an essential role in the antimicrobial and antiviral system of humans. The microbicidal activity of MPO exists due to its capability to oxidize halide and pseudohalide ions (Cl-, Br-, I- and SCN-) by H2O2, thereby producing respective hypohalous acids (HOX). During the phagocytosis of pathogens, azurophilic granules release their content together with MPO into phagolysosomes. On the other hand, MPO can be discharged outside the phagocytes. Due to this, tissue damage during inflammation is greatly promoted by MPO-derived oxidants. Regarding its activity, MPO is a key factor in a great number of conditions within the group of cardiovascular diseases, inflammatory diseases, neurodegenerative diseases, kidney diseases and immune-mediated diseases. Therefore, MPO and its downstream inflammatory pathways might be attractive targets for both prognostic and therapeutic intervention in the prophylaxis of all mentioned illnesses. Nowadays, structure and reaction mechanism of MPO are known, which enable rational strategy in the development of specific MPO inhibitors that still preserve MPO activity during host defense from bacteria, but hinder pathophysiologically persistent activation of MPO. Various methods for MPO activity inhibition and unfavorable effects of MPO-derived oxidants remodeling will be discussed. Emphasis will be put on various known inhibitors, as well as on newly investigated natural products, which can also inhibit MPO activity.
T2  - Current Drug Metabolism
T1  - Myeloperoxidase Inhibitors as Potential Drugs
VL  - 16
IS  - 3
SP  - 168
EP  - 190
DO  - 10.2174/138920021603150812120640
ER  - 
@article{
author = "Lazarević-Pašti, Tamara and Leskovac, Andreja and Vasić, Vesna M.",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/700",
abstract = "Myeloperoxidase (MPO) is an important member of the haem peroxidase - cyclooxygenase superfamily. This enzyme is physiologically expressed in circulating neutrophils, monocytes and some tissue macrophages including microglia. MPO plays an essential role in the antimicrobial and antiviral system of humans. The microbicidal activity of MPO exists due to its capability to oxidize halide and pseudohalide ions (Cl-, Br-, I- and SCN-) by H2O2, thereby producing respective hypohalous acids (HOX). During the phagocytosis of pathogens, azurophilic granules release their content together with MPO into phagolysosomes. On the other hand, MPO can be discharged outside the phagocytes. Due to this, tissue damage during inflammation is greatly promoted by MPO-derived oxidants. Regarding its activity, MPO is a key factor in a great number of conditions within the group of cardiovascular diseases, inflammatory diseases, neurodegenerative diseases, kidney diseases and immune-mediated diseases. Therefore, MPO and its downstream inflammatory pathways might be attractive targets for both prognostic and therapeutic intervention in the prophylaxis of all mentioned illnesses. Nowadays, structure and reaction mechanism of MPO are known, which enable rational strategy in the development of specific MPO inhibitors that still preserve MPO activity during host defense from bacteria, but hinder pathophysiologically persistent activation of MPO. Various methods for MPO activity inhibition and unfavorable effects of MPO-derived oxidants remodeling will be discussed. Emphasis will be put on various known inhibitors, as well as on newly investigated natural products, which can also inhibit MPO activity.",
journal = "Current Drug Metabolism",
title = "Myeloperoxidase Inhibitors as Potential Drugs",
volume = "16",
number = "3",
pages = "168-190",
doi = "10.2174/138920021603150812120640"
}
Lazarević-Pašti, T., Leskovac, A.,& Vasić, V. M. (2015). Myeloperoxidase Inhibitors as Potential Drugs.
Current Drug Metabolism, 16(3), 168-190.
https://doi.org/10.2174/138920021603150812120640
Lazarević-Pašti T, Leskovac A, Vasić VM. Myeloperoxidase Inhibitors as Potential Drugs. Current Drug Metabolism. 2015;16(3):168-190
Lazarević-Pašti Tamara, Leskovac Andreja, Vasić Vesna M., "Myeloperoxidase Inhibitors as Potential Drugs" Current Drug Metabolism, 16, no. 3 (2015):168-190,
https://doi.org/10.2174/138920021603150812120640 .
3
53
44
52

Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: A histopathological study

Martinović, Jelena; Todorović, Nevena; Tomanovic, Nada; Bošković, Maja; Djordjevic, Snezana; Lazarević-Pašti, Tamara; Bernardi, Rick E.; Djurdjevic, Aleksandra; Filipović, Dragana

(2014)

TY  - JOUR
AU  - Martinović, Jelena
AU  - Todorović, Nevena
AU  - Tomanovic, Nada
AU  - Bošković, Maja
AU  - Djordjevic, Snezana
AU  - Lazarević-Pašti, Tamara
AU  - Bernardi, Rick E.
AU  - Djurdjevic, Aleksandra
AU  - Filipović, Dragana
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6043
AB  - Chronic exposure to stress contributes to the etiology of mood disorders, and the liver as a target organ of antidepressant and antipsychotic drug metabolism is vulnerable to drug-induced toxicity. We investigated the effects of chronic administration of fluoxetine (15 mg/kg/day) or clozapine (20 mg/kg/day) on liver injury via the measurement of liver enzymes, oxidative stress and histopathology in rats exposed to chronic social isolation (21 days), an animal model of depression, and controls. The activity of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the liver content of carbonyl groups, malonyldialdehyde (MDA), reduced glutathione (GSH), cytosolic glutathione S-transferase (GST) and nitric oxide (NO) metabolites were determined. We also characterized nuclear factor-kappa B (NF-kappa B), cyclooxygenase-2 (COX-2) and CuZn-superoxide dismutase (CuZnSOD) protein expression as well as histopathological changes. Increased serum ALT activity in chronically-isolated and control animals treated with both drugs was found while increased AST activity was observed only in fluoxetine-treated rats (chronically-isolated and controls). Increased carbonyl content, MDA, GST activity and decreased GSH levels in drug-treated controls/chronically-isolated animals suggest a link between drugs and hepatic oxidative stress. Increased NO levels associated with NF-kappa B activation and the concomitant increased COX-2 expression together with compromised CuZnSOD expression in clozapine-treated chronically-isolated rats likely reinforce oxidative stress, observed by increased lipid peroxidation and GSH depletion. In contrast, fluoxetine reduced NO levels in chronically-isolated rats. Isolation induced oxidative stress but histological changes were similar to those observed in vehicle-treated controls. Chronic administration of fluoxetine in both chronically-isolated and control animals resulted in more or less normal hepatic architecture, while clozapine in both groups resulted in liver injury. These data suggest that clozapine appears to have a higher potential to induce liver toxicity than fluoxetine. (C) 2014 Elsevier B.V. All rights reserved.
T2  - European Journal of Pharmaceutical Sciences
T1  - Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: A histopathological study
VL  - 59
SP  - 20
EP  - 30
DO  - 10.1016/j.ejps.2014.04.010
ER  - 
@article{
author = "Martinović, Jelena and Todorović, Nevena and Tomanovic, Nada and Bošković, Maja and Djordjevic, Snezana and Lazarević-Pašti, Tamara and Bernardi, Rick E. and Djurdjevic, Aleksandra and Filipović, Dragana",
year = "2014",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/6043",
abstract = "Chronic exposure to stress contributes to the etiology of mood disorders, and the liver as a target organ of antidepressant and antipsychotic drug metabolism is vulnerable to drug-induced toxicity. We investigated the effects of chronic administration of fluoxetine (15 mg/kg/day) or clozapine (20 mg/kg/day) on liver injury via the measurement of liver enzymes, oxidative stress and histopathology in rats exposed to chronic social isolation (21 days), an animal model of depression, and controls. The activity of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the liver content of carbonyl groups, malonyldialdehyde (MDA), reduced glutathione (GSH), cytosolic glutathione S-transferase (GST) and nitric oxide (NO) metabolites were determined. We also characterized nuclear factor-kappa B (NF-kappa B), cyclooxygenase-2 (COX-2) and CuZn-superoxide dismutase (CuZnSOD) protein expression as well as histopathological changes. Increased serum ALT activity in chronically-isolated and control animals treated with both drugs was found while increased AST activity was observed only in fluoxetine-treated rats (chronically-isolated and controls). Increased carbonyl content, MDA, GST activity and decreased GSH levels in drug-treated controls/chronically-isolated animals suggest a link between drugs and hepatic oxidative stress. Increased NO levels associated with NF-kappa B activation and the concomitant increased COX-2 expression together with compromised CuZnSOD expression in clozapine-treated chronically-isolated rats likely reinforce oxidative stress, observed by increased lipid peroxidation and GSH depletion. In contrast, fluoxetine reduced NO levels in chronically-isolated rats. Isolation induced oxidative stress but histological changes were similar to those observed in vehicle-treated controls. Chronic administration of fluoxetine in both chronically-isolated and control animals resulted in more or less normal hepatic architecture, while clozapine in both groups resulted in liver injury. These data suggest that clozapine appears to have a higher potential to induce liver toxicity than fluoxetine. (C) 2014 Elsevier B.V. All rights reserved.",
journal = "European Journal of Pharmaceutical Sciences",
title = "Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: A histopathological study",
volume = "59",
pages = "20-30",
doi = "10.1016/j.ejps.2014.04.010"
}
Martinović, J., Todorović, N., Tomanovic, N., Bošković, M., Djordjevic, S., Lazarević-Pašti, T., Bernardi, R. E., Djurdjevic, A.,& Filipović, D. (2014). Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: A histopathological study.
European Journal of Pharmaceutical Sciences, 59, 20-30.
https://doi.org/10.1016/j.ejps.2014.04.010
Martinović J, Todorović N, Tomanovic N, Bošković M, Djordjevic S, Lazarević-Pašti T, Bernardi RE, Djurdjevic A, Filipović D. Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: A histopathological study. European Journal of Pharmaceutical Sciences. 2014;59:20-30
Martinović Jelena, Todorović Nevena, Tomanovic Nada, Bošković Maja, Djordjevic Snezana, Lazarević-Pašti Tamara, Bernardi Rick E., Djurdjevic Aleksandra, Filipović Dragana, "Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: A histopathological study" European Journal of Pharmaceutical Sciences, 59 (2014):20-30,
https://doi.org/10.1016/j.ejps.2014.04.010 .
37
34
36

Electrochemical oxidation of diazinon in aqueous solutions via electrogenerated halogens - Diazinon fate and implications for its detection

Lazarević-Pašti, Tamara; Bondžić, Aleksandra; Pašti, Igor A.; Mentus, Slavko V.; Vasić, Vesna M.

(2013)

TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Bondžić, Aleksandra
AU  - Pašti, Igor A.
AU  - Mentus, Slavko V.
AU  - Vasić, Vesna M.
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5410
AB  - Organophosphorous pesticide diazinon was indirectly oxidized to diazoxon by means of electrogenerated Cl-2, Br-2 or I-2 under galvanostatic conditions using rotating glassy carbon disk electrode. The rate of oxidation was the highest in the case of electrogenerated Br-2. In the presence of electrogenerated Br-2 diazinon was completely converted to diazoxon after 15 min of electrochemical treatment at all concentrations investigated. On the basis of thermodynamic and kinetic arguments it was showed that diazinon oxidation is governed by both kinetics and speciation of halogen species. Diazoxon is rapidly formed during its reaction with active oxidant species, while formed diazoxon is being stable and not degraded at appreciable rate by reactive halogen species. Described oxidation procedure was used as a pre-step for diazinon detection using AChE test coupled with optical detection. Diazinon detection limit was reduced by three orders of magnitude compared to unoxidized diazinon. This enabled diazinon detection at the lowest possible concentration using described methodology, as determined by diazoxon detection limit. (C) 2013 Elsevier B.V. All rights reserved.
T2  - Journal of Electroanalytical Chemistry
T1  - Electrochemical oxidation of diazinon in aqueous solutions via electrogenerated halogens - Diazinon fate and implications for its detection
VL  - 692
SP  - 40
EP  - 45
DO  - 10.1016/j.jelechem.2013.01.005
ER  - 
@article{
author = "Lazarević-Pašti, Tamara and Bondžić, Aleksandra and Pašti, Igor A. and Mentus, Slavko V. and Vasić, Vesna M.",
year = "2013",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5410",
abstract = "Organophosphorous pesticide diazinon was indirectly oxidized to diazoxon by means of electrogenerated Cl-2, Br-2 or I-2 under galvanostatic conditions using rotating glassy carbon disk electrode. The rate of oxidation was the highest in the case of electrogenerated Br-2. In the presence of electrogenerated Br-2 diazinon was completely converted to diazoxon after 15 min of electrochemical treatment at all concentrations investigated. On the basis of thermodynamic and kinetic arguments it was showed that diazinon oxidation is governed by both kinetics and speciation of halogen species. Diazoxon is rapidly formed during its reaction with active oxidant species, while formed diazoxon is being stable and not degraded at appreciable rate by reactive halogen species. Described oxidation procedure was used as a pre-step for diazinon detection using AChE test coupled with optical detection. Diazinon detection limit was reduced by three orders of magnitude compared to unoxidized diazinon. This enabled diazinon detection at the lowest possible concentration using described methodology, as determined by diazoxon detection limit. (C) 2013 Elsevier B.V. All rights reserved.",
journal = "Journal of Electroanalytical Chemistry",
title = "Electrochemical oxidation of diazinon in aqueous solutions via electrogenerated halogens - Diazinon fate and implications for its detection",
volume = "692",
pages = "40-45",
doi = "10.1016/j.jelechem.2013.01.005"
}
Lazarević-Pašti, T., Bondžić, A., Pašti, I. A., Mentus, S. V.,& Vasić, V. M. (2013). Electrochemical oxidation of diazinon in aqueous solutions via electrogenerated halogens - Diazinon fate and implications for its detection.
Journal of Electroanalytical Chemistry, 692, 40-45.
https://doi.org/10.1016/j.jelechem.2013.01.005
Lazarević-Pašti T, Bondžić A, Pašti IA, Mentus SV, Vasić VM. Electrochemical oxidation of diazinon in aqueous solutions via electrogenerated halogens - Diazinon fate and implications for its detection. Journal of Electroanalytical Chemistry. 2013;692:40-45
Lazarević-Pašti Tamara, Bondžić Aleksandra, Pašti Igor A., Mentus Slavko V., Vasić Vesna M., "Electrochemical oxidation of diazinon in aqueous solutions via electrogenerated halogens - Diazinon fate and implications for its detection" Journal of Electroanalytical Chemistry, 692 (2013):40-45,
https://doi.org/10.1016/j.jelechem.2013.01.005 .
15
14
19

Acetylcholinesterase Inhibitors: Pharmacology and Toxicology

Čolović, Mirjana B.; Krstić, Danijela Z.; Lazarević-Pašti, Tamara; Bondžić, Aleksandra; Vasić, Vesna M.

(2013)

TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Lazarević-Pašti, Tamara
AU  - Bondžić, Aleksandra
AU  - Vasić, Vesna M.
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5474
AB  - Acetylcholinesterase is involved in the termination of impulse transmission by rapid hydrolysis of the neurotransmitter acetylcholine in numerous cholinergic pathways in the central and peripheral nervous systems. The enzyme inactivation, induced by various inhibitors, leads to acetylcholine accumulation, hyperstimulation of nicotinic and muscarinic receptors, and disrupted neurotransmission. Hence, acetylcholinesterase inhibitors, interacting with the enzyme as their primary target, are applied as relevant drugs and toxins. This review presents an overview of toxicology and pharmacology of reversible and irreversible acetylcholinesterase inactivating compounds. In the case of reversible inhibitors being commonly applied in neurodegenerative disorders treatment, special attention is paid to currently approved drugs (donepezil, rivastigmine and galantamine) in the pharmacotherapy of Alzheimers disease, and toxic carbamates used as pesticides. Subsequently, mechanism of irreversible acetylcholinesterase inhibition induced by organophosphorus compounds (insecticides and nerve agents), and their specific and nonspecific toxic effects are described, as well as irreversible inhibitors having pharmacological implementation. In addition, the pharmacological treatment of intoxication caused by organophosphates is presented, with emphasis on oxime reactivators of the inhibited enzyme activity administering as causal drugs after the poisoning. Besides, organophosphorus and carbamate insecticides can be detoxified in mammals through enzymatic hydrolysis before they reach targets in the nervous system. Carboxylesterases most effectively decompose carbamates, whereas the most successful route of organophosphates detoxification is their degradation by corresponding phosphotriesterases.
T2  - Current Neuropharmacology
T1  - Acetylcholinesterase Inhibitors: Pharmacology and Toxicology
VL  - 11
IS  - 3
SP  - 315
EP  - 335
DO  - 10.2174/1570159X11311030006
ER  - 
@article{
author = "Čolović, Mirjana B. and Krstić, Danijela Z. and Lazarević-Pašti, Tamara and Bondžić, Aleksandra and Vasić, Vesna M.",
year = "2013",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5474",
abstract = "Acetylcholinesterase is involved in the termination of impulse transmission by rapid hydrolysis of the neurotransmitter acetylcholine in numerous cholinergic pathways in the central and peripheral nervous systems. The enzyme inactivation, induced by various inhibitors, leads to acetylcholine accumulation, hyperstimulation of nicotinic and muscarinic receptors, and disrupted neurotransmission. Hence, acetylcholinesterase inhibitors, interacting with the enzyme as their primary target, are applied as relevant drugs and toxins. This review presents an overview of toxicology and pharmacology of reversible and irreversible acetylcholinesterase inactivating compounds. In the case of reversible inhibitors being commonly applied in neurodegenerative disorders treatment, special attention is paid to currently approved drugs (donepezil, rivastigmine and galantamine) in the pharmacotherapy of Alzheimers disease, and toxic carbamates used as pesticides. Subsequently, mechanism of irreversible acetylcholinesterase inhibition induced by organophosphorus compounds (insecticides and nerve agents), and their specific and nonspecific toxic effects are described, as well as irreversible inhibitors having pharmacological implementation. In addition, the pharmacological treatment of intoxication caused by organophosphates is presented, with emphasis on oxime reactivators of the inhibited enzyme activity administering as causal drugs after the poisoning. Besides, organophosphorus and carbamate insecticides can be detoxified in mammals through enzymatic hydrolysis before they reach targets in the nervous system. Carboxylesterases most effectively decompose carbamates, whereas the most successful route of organophosphates detoxification is their degradation by corresponding phosphotriesterases.",
journal = "Current Neuropharmacology",
title = "Acetylcholinesterase Inhibitors: Pharmacology and Toxicology",
volume = "11",
number = "3",
pages = "315-335",
doi = "10.2174/1570159X11311030006"
}
Čolović, M. B., Krstić, D. Z., Lazarević-Pašti, T., Bondžić, A.,& Vasić, V. M. (2013). Acetylcholinesterase Inhibitors: Pharmacology and Toxicology.
Current Neuropharmacology, 11(3), 315-335.
https://doi.org/10.2174/1570159X11311030006
Čolović MB, Krstić DZ, Lazarević-Pašti T, Bondžić A, Vasić VM. Acetylcholinesterase Inhibitors: Pharmacology and Toxicology. Current Neuropharmacology. 2013;11(3):315-335
Čolović Mirjana B., Krstić Danijela Z., Lazarević-Pašti Tamara, Bondžić Aleksandra, Vasić Vesna M., "Acetylcholinesterase Inhibitors: Pharmacology and Toxicology" Current Neuropharmacology, 11, no. 3 (2013):315-335,
https://doi.org/10.2174/1570159X11311030006 .
75
1013
862
978

Influence of organophosphorus pesticides on peroxidase and chlorination activity of human myeloperoxidase

Lazarević-Pašti, Tamara; Momić, Tatjana; Radojevic, Milos M.; Vasić, Vesna M.

(2013)

TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Momić, Tatjana
AU  - Radojevic, Milos M.
AU  - Vasić, Vesna M.
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5650
AB  - Inhibitory effects of five organophosphorus pesticides (diazinon, malathion, chlorpyrifos, azinphosmethyl and phorate) and their oxo-analogs on human myeloperoxidase (MPO) activity were investigated. While inspecting separately peroxidase and chlorination activity, it was observed that investigated OPs affect peroxidase activity, but not chlorination activity. Among investigated pesticides, malathion and malaoxon have showed the highest power to inhibit MPO peroxidase activity With IC50 values of the order of 3 x 10(-7) and 5 x 10(-9) M, respectively. It was proposed that inhibition trend is rendered by molecular structure which invokes steric hindrance for OPs interaction with MPO active center responsible for peroxidase activity. In addition, it was concluded that physiological function of MPO is not affected by any of the investigated OPs. (C) 2013 Elsevier Inc. All rights reserved.
T2  - Pesticide Biochemistry and Physiology
T1  - Influence of organophosphorus pesticides on peroxidase and chlorination activity of human myeloperoxidase
VL  - 107
IS  - 1
SP  - 55
EP  - 60
DO  - 10.1016/j.pestbp.2013.05.004
ER  - 
@article{
author = "Lazarević-Pašti, Tamara and Momić, Tatjana and Radojevic, Milos M. and Vasić, Vesna M.",
year = "2013",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5650",
abstract = "Inhibitory effects of five organophosphorus pesticides (diazinon, malathion, chlorpyrifos, azinphosmethyl and phorate) and their oxo-analogs on human myeloperoxidase (MPO) activity were investigated. While inspecting separately peroxidase and chlorination activity, it was observed that investigated OPs affect peroxidase activity, but not chlorination activity. Among investigated pesticides, malathion and malaoxon have showed the highest power to inhibit MPO peroxidase activity With IC50 values of the order of 3 x 10(-7) and 5 x 10(-9) M, respectively. It was proposed that inhibition trend is rendered by molecular structure which invokes steric hindrance for OPs interaction with MPO active center responsible for peroxidase activity. In addition, it was concluded that physiological function of MPO is not affected by any of the investigated OPs. (C) 2013 Elsevier Inc. All rights reserved.",
journal = "Pesticide Biochemistry and Physiology",
title = "Influence of organophosphorus pesticides on peroxidase and chlorination activity of human myeloperoxidase",
volume = "107",
number = "1",
pages = "55-60",
doi = "10.1016/j.pestbp.2013.05.004"
}
Lazarević-Pašti, T., Momić, T., Radojevic, M. M.,& Vasić, V. M. (2013). Influence of organophosphorus pesticides on peroxidase and chlorination activity of human myeloperoxidase.
Pesticide Biochemistry and Physiology, 107(1), 55-60.
https://doi.org/10.1016/j.pestbp.2013.05.004
Lazarević-Pašti T, Momić T, Radojevic MM, Vasić VM. Influence of organophosphorus pesticides on peroxidase and chlorination activity of human myeloperoxidase. Pesticide Biochemistry and Physiology. 2013;107(1):55-60
Lazarević-Pašti Tamara, Momić Tatjana, Radojevic Milos M., Vasić Vesna M., "Influence of organophosphorus pesticides on peroxidase and chlorination activity of human myeloperoxidase" Pesticide Biochemistry and Physiology, 107, no. 1 (2013):55-60,
https://doi.org/10.1016/j.pestbp.2013.05.004 .
5
5
8

The Antiradical, Anti-Inflammatory and Anti-Genotoxic Potential of Herbal Preparation Chlamyfin

Leskovac, Andreja; Joksić, Gordana; Pašti, Igor A.; Lazarević-Pašti, Tamara; Nastasijević, Branislav J.; Petrović, Sandra

(2013)

TY  - JOUR
AU  - Leskovac, Andreja
AU  - Joksić, Gordana
AU  - Pašti, Igor A.
AU  - Lazarević-Pašti, Tamara
AU  - Nastasijević, Branislav J.
AU  - Petrović, Sandra
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5830
AB  - Herbal preparation Chlamyfin was investigated for its total polyphenol content, 2,2-diphenyl-1picrylhydrazyl (DPPH) scavenging activity, and anti-inflammatory and genotoxic properties. A high total polyphenol content provided evidence of high DPPH radical scavenging activity (IC50 = 4.96 +/- 0.23 mu g/ml). Analysis of the electrochemical behavior of Chlamyfin indicated high reducing ability, i.e., high antioxidant capacity, in agreement with the DPPH test. Analysis of myeloperoxidase (MPO) inhibition by Chlamyfin suggested anti-inflammatory action (IC50 values of 5.40 mu g/ml and 4.45 i_tg/m1 for an incubation time of 10 and 30 min, respectively). For genotoxic assessment, oxidative stress was induced by irradiation of peripheral whole blood with gamma-radiation in vitro. In the presence of Chlamyfin, reduced incidence of micronuclei without disturbance to the proliferative potential of cells was evidenced in both irradiated and unirradiated samples, indicating its genoprotective properties. It was shown that Chlamyfm, in addition to its bactericidal effect, also possesses strong antioxidant, anti-inflammatory and anti-genotoxic properties.
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - The Antiradical, Anti-Inflammatory and Anti-Genotoxic Potential of Herbal Preparation Chlamyfin
VL  - 32
IS  - 2
SP  - 227
EP  - 237
ER  - 
@article{
author = "Leskovac, Andreja and Joksić, Gordana and Pašti, Igor A. and Lazarević-Pašti, Tamara and Nastasijević, Branislav J. and Petrović, Sandra",
year = "2013",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5830",
abstract = "Herbal preparation Chlamyfin was investigated for its total polyphenol content, 2,2-diphenyl-1picrylhydrazyl (DPPH) scavenging activity, and anti-inflammatory and genotoxic properties. A high total polyphenol content provided evidence of high DPPH radical scavenging activity (IC50 = 4.96 +/- 0.23 mu g/ml). Analysis of the electrochemical behavior of Chlamyfin indicated high reducing ability, i.e., high antioxidant capacity, in agreement with the DPPH test. Analysis of myeloperoxidase (MPO) inhibition by Chlamyfin suggested anti-inflammatory action (IC50 values of 5.40 mu g/ml and 4.45 i_tg/m1 for an incubation time of 10 and 30 min, respectively). For genotoxic assessment, oxidative stress was induced by irradiation of peripheral whole blood with gamma-radiation in vitro. In the presence of Chlamyfin, reduced incidence of micronuclei without disturbance to the proliferative potential of cells was evidenced in both irradiated and unirradiated samples, indicating its genoprotective properties. It was shown that Chlamyfm, in addition to its bactericidal effect, also possesses strong antioxidant, anti-inflammatory and anti-genotoxic properties.",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "The Antiradical, Anti-Inflammatory and Anti-Genotoxic Potential of Herbal Preparation Chlamyfin",
volume = "32",
number = "2",
pages = "227-237"
}
Leskovac, A., Joksić, G., Pašti, I. A., Lazarević-Pašti, T., Nastasijević, B. J.,& Petrović, S. (2013). The Antiradical, Anti-Inflammatory and Anti-Genotoxic Potential of Herbal Preparation Chlamyfin.
Macedonian Journal of Chemistry and Chemical Engineering, 32(2), 227-237.
Leskovac A, Joksić G, Pašti IA, Lazarević-Pašti T, Nastasijević BJ, Petrović S. The Antiradical, Anti-Inflammatory and Anti-Genotoxic Potential of Herbal Preparation Chlamyfin. Macedonian Journal of Chemistry and Chemical Engineering. 2013;32(2):227-237
Leskovac Andreja, Joksić Gordana, Pašti Igor A., Lazarević-Pašti Tamara, Nastasijević Branislav J., Petrović Sandra, "The Antiradical, Anti-Inflammatory and Anti-Genotoxic Potential of Herbal Preparation Chlamyfin" Macedonian Journal of Chemistry and Chemical Engineering, 32, no. 2 (2013):227-237
4

Investigation of reaction between quercetin and Au(III) in acidic media: mechanism and identification of reaction products

Bondžić, Aleksandra; Lazarević-Pašti, Tamara; Bondzic, Bojan P.; Čolović, Mirjana B.; Jadranin, Milka B.; Vasić, Vesna M.

(2013)

TY  - JOUR
AU  - Bondžić, Aleksandra
AU  - Lazarević-Pašti, Tamara
AU  - Bondzic, Bojan P.
AU  - Čolović, Mirjana B.
AU  - Jadranin, Milka B.
AU  - Vasić, Vesna M.
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5362
AB  - The aim of the present paper was to investigate the reaction of quercetin, the flavonol very often used as a dietary supplement, with [AuCl4](-) ions. The reaction was studied spectrophotometrically using the equimolar solutions in 1 : 1 water-methanol at pH similar to 2. The spectrophotometric data indicated the formation of the products with an absorption maximum at 295 nm in all cases, characteristic of the oxidized forms of quercetin. HPLC coupled with DAD and LC-MS analysis of the reaction products suggested that the oxidation of quercetin resulted in the generation of similar metabolites including quinone and various oxidized quercetin-solvent adducts. In addition, cyclic voltammetric measurements confirmed that under applied experimental conditions, the reduction of Au(III) to Au(0) took place. The reduction species in the reaction mixture were Au(III) ions, while Au(I) disproportionates back to Au(III) and Au(0). The newly generated Au(III) ions further oxidized 3-4-dihydroxy groups of quercetin adducts obtained after first 2e(-) oxidation, giving the final reaction products. Based on the identification of reaction products, the reaction mechanism for the oxidation of quercetin in the presence of Au(III) which involves two 2e(-) transfer processes was proposed.
T2  - New Journal of Chemistry
T1  - Investigation of reaction between quercetin and Au(III) in acidic media: mechanism and identification of reaction products
VL  - 37
IS  - 4
SP  - 901
EP  - 908
DO  - 10.1039/c2nj40742f
ER  - 
@article{
author = "Bondžić, Aleksandra and Lazarević-Pašti, Tamara and Bondzic, Bojan P. and Čolović, Mirjana B. and Jadranin, Milka B. and Vasić, Vesna M.",
year = "2013",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5362",
abstract = "The aim of the present paper was to investigate the reaction of quercetin, the flavonol very often used as a dietary supplement, with [AuCl4](-) ions. The reaction was studied spectrophotometrically using the equimolar solutions in 1 : 1 water-methanol at pH similar to 2. The spectrophotometric data indicated the formation of the products with an absorption maximum at 295 nm in all cases, characteristic of the oxidized forms of quercetin. HPLC coupled with DAD and LC-MS analysis of the reaction products suggested that the oxidation of quercetin resulted in the generation of similar metabolites including quinone and various oxidized quercetin-solvent adducts. In addition, cyclic voltammetric measurements confirmed that under applied experimental conditions, the reduction of Au(III) to Au(0) took place. The reduction species in the reaction mixture were Au(III) ions, while Au(I) disproportionates back to Au(III) and Au(0). The newly generated Au(III) ions further oxidized 3-4-dihydroxy groups of quercetin adducts obtained after first 2e(-) oxidation, giving the final reaction products. Based on the identification of reaction products, the reaction mechanism for the oxidation of quercetin in the presence of Au(III) which involves two 2e(-) transfer processes was proposed.",
journal = "New Journal of Chemistry",
title = "Investigation of reaction between quercetin and Au(III) in acidic media: mechanism and identification of reaction products",
volume = "37",
number = "4",
pages = "901-908",
doi = "10.1039/c2nj40742f"
}
Bondžić, A., Lazarević-Pašti, T., Bondzic, B. P., Čolović, M. B., Jadranin, M. B.,& Vasić, V. M. (2013). Investigation of reaction between quercetin and Au(III) in acidic media: mechanism and identification of reaction products.
New Journal of Chemistry, 37(4), 901-908.
https://doi.org/10.1039/c2nj40742f
Bondžić A, Lazarević-Pašti T, Bondzic BP, Čolović MB, Jadranin MB, Vasić VM. Investigation of reaction between quercetin and Au(III) in acidic media: mechanism and identification of reaction products. New Journal of Chemistry. 2013;37(4):901-908
Bondžić Aleksandra, Lazarević-Pašti Tamara, Bondzic Bojan P., Čolović Mirjana B., Jadranin Milka B., Vasić Vesna M., "Investigation of reaction between quercetin and Au(III) in acidic media: mechanism and identification of reaction products" New Journal of Chemistry, 37, no. 4 (2013):901-908,
https://doi.org/10.1039/c2nj40742f .
13
15
16

Switching between voltammetry and potentiometry in order to determine H+ or OH- ion concentration over the entire pH scale by means of tungsten disk electrode

Pašti, Igor A.; Lazarević-Pašti, Tamara; Mentus, Slavko V.

(2012)

TY  - JOUR
AU  - Pašti, Igor A.
AU  - Lazarević-Pašti, Tamara
AU  - Mentus, Slavko V.
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4704
AB  - This study demonstrates that tungsten rotating disk electrode can be used for pH or H+/OH- concentration measurements in practically entire pH range, using the combination of voltammetric and potentiometric techniques. In strong acidic (c(H+) GT 10(-3) mol dm(-3)), and alkaline (c(OH-) GT 10(-3) mol dm(-3)) solutions, rotating tungsten disk electrode can be used as a maintenance-free electrode for voltammetric determination of H+ and OH- ion concentration. After an appropriate electrochemical oxidation, the same electrode, in a stationary regime, may be used as a potentiometric sensor in the range 3 LT pH LT 11, with the response -49.5 +/- 2.3 mV per one pH unit. (C) 2011 Elsevier B.V. All rights reserved.
T2  - Journal of Electroanalytical Chemistry
T1  - Switching between voltammetry and potentiometry in order to determine H+ or OH- ion concentration over the entire pH scale by means of tungsten disk electrode
VL  - 665
SP  - 83
EP  - 89
DO  - 10.1016/j.jelechem.2011.11.019
ER  - 
@article{
author = "Pašti, Igor A. and Lazarević-Pašti, Tamara and Mentus, Slavko V.",
year = "2012",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4704",
abstract = "This study demonstrates that tungsten rotating disk electrode can be used for pH or H+/OH- concentration measurements in practically entire pH range, using the combination of voltammetric and potentiometric techniques. In strong acidic (c(H+) GT 10(-3) mol dm(-3)), and alkaline (c(OH-) GT 10(-3) mol dm(-3)) solutions, rotating tungsten disk electrode can be used as a maintenance-free electrode for voltammetric determination of H+ and OH- ion concentration. After an appropriate electrochemical oxidation, the same electrode, in a stationary regime, may be used as a potentiometric sensor in the range 3 LT pH LT 11, with the response -49.5 +/- 2.3 mV per one pH unit. (C) 2011 Elsevier B.V. All rights reserved.",
journal = "Journal of Electroanalytical Chemistry",
title = "Switching between voltammetry and potentiometry in order to determine H+ or OH- ion concentration over the entire pH scale by means of tungsten disk electrode",
volume = "665",
pages = "83-89",
doi = "10.1016/j.jelechem.2011.11.019"
}
Pašti, I. A., Lazarević-Pašti, T.,& Mentus, S. V. (2012). Switching between voltammetry and potentiometry in order to determine H+ or OH- ion concentration over the entire pH scale by means of tungsten disk electrode.
Journal of Electroanalytical Chemistry, 665, 83-89.
https://doi.org/10.1016/j.jelechem.2011.11.019
Pašti IA, Lazarević-Pašti T, Mentus SV. Switching between voltammetry and potentiometry in order to determine H+ or OH- ion concentration over the entire pH scale by means of tungsten disk electrode. Journal of Electroanalytical Chemistry. 2012;665:83-89
Pašti Igor A., Lazarević-Pašti Tamara, Mentus Slavko V., "Switching between voltammetry and potentiometry in order to determine H+ or OH- ion concentration over the entire pH scale by means of tungsten disk electrode" Journal of Electroanalytical Chemistry, 665 (2012):83-89,
https://doi.org/10.1016/j.jelechem.2011.11.019 .
13
10
13

Primena oksidacije organo-tiofosfatnih pesticida u metodama za njihovu detekciju na bazi inhibicije acetilholinesteraze

Lazarević-Pašti, Tamara

(Универзитет у Београду, Хемијски факултет, 2012)

TY  - BOOK
AU  - Lazarević-Pašti, Tamara
PY  - 2012
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=332
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:5873/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=41104655
UR  - http://nardus.mpn.gov.rs/123456789/3468
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7267
AB  - Ispitana je inhibicija slobodne acetilholinesteraze (AChE) odabranim organotiofosfatima (OP) (diazinon, malation, hlorpirifos, azinfos-metil, forat) i njihovim oksoanalozima (diazokson, malaokson, hlorpirifos-okson, azinfos-metil-okson, forat-okson), pri čemu su optimizovani uslovi za detekciju najniže koncentracije tih jedinjenja primenom AChE testa. Određene su IC50 vrednosti za sva navedena jedinjenja. U cilju povećanja osetljivosti AChE testa, ispitivani organo-tiofosfati prevedeni su u okso-analoge u prisustvu enzima mijeloperoksidaze (MPO). To je potvreno pomoćuUPLC i GC/MS analize. Nastali oksidacioni proizvodi stabilni su najmanje 1h. Maksimalne koncentracije oksona dobijaju se kada se organo-tiofosfati inkubiraju sa 100 nM MPO u prisustvu H2O2 koncentracije 50 μM, pri pH 6, na temperaturi 25 °C u toku 10 minuta. Oksidacija OP u prisustvu MPO pod navedenim uslovima primenjena je u modifikaciji metode za detekciju OP na bazi inhibicije AChE. Određena je granica detekcije modifikovane metode, kao i efikasnost oksidacije i njena primena na smešu OP. Gore navedeni organo-tiofosfati prevedeni su u odgovarajue oksone i pomoću elektrohemijski generisanih halogena. Proizvodi koji nastaju identifikovani su kao oksoni pomoću UPLC analize. Utvrđeno je da je najefikasnija oksidacija elektrogenerisanim bromom u toku 15 minuta. Određena je i granica detekcije AChE testa za odreivanje OP kada su OP oksidovani elektrohemijski generisanim bromom...
AB  - The inhibition of acetylcholinesterase in the presence of selected organothiophosphates (diazinon, malathion, chlorpyrifos, azinphos-methyl, phorate) andtheir oxo-analogues (diazoxon, malaoxon, chlorpyrifos-oxon, azinphos-methyl-oxon,phorate-oxon) was examined. Experimental conditions were optimized to detect the lowestposible concentrations of these compounds using AChE test. IC50 values were determinedfor all the mentioned compounds.Investigated organothiophosphates have been converted into their oxo-analogues inthe presence of the enzyme myeloperoxidase. Oxidation products were detected usingUPLC and GC/MS analysis. It was found that the products are stable within minimum 1h.In order to optimize oxidation procedure and achieve maximum concentrations of oxons,the optimal concentrations of H2O2 (50 μM) and MPO (100 nM) were determined. Also,the optimal incubation time of OPs and MPO (10 min), as well as the optimal pH (6) andtemperature (25 °C) were estimated. Oxidation of OPs in the presence of MPO underoptimal conditions was applied as a modification of the method for detecting OP usingAChE test. The detection limits were determined for the modified method, as well as theefficiency of oxidation and its application for the analysi of sintetic mixture of OPs.Examined organothiophosphates have been converted into their oxo-forms usingelectrochemically generated halogens. Products have been identified as oxons using UPLCanalysis. It was found that the the most efficient oxidation of OPs was carried out by after15 minutes of oxidation with electrogenerated bromine. Detection limits were determined,too.
PB  - Универзитет у Београду, Хемијски факултет
T2  - Универзитет у Београду
T1  - Primena oksidacije organo-tiofosfatnih pesticida u metodama za njihovu detekciju na bazi inhibicije acetilholinesteraze
T1  - Application of organothiophosphate pesticides oxidation in methods for their detection based on acetylcholinesterase inhibition
ER  - 
@phdthesis{
author = "Lazarević-Pašti, Tamara",
year = "2012",
url = "http://eteze.bg.ac.rs/application/showtheses?thesesId=332, https://fedorabg.bg.ac.rs/fedora/get/o:5873/bdef:Content/download, http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=41104655, http://nardus.mpn.gov.rs/123456789/3468, http://vinar.vin.bg.ac.rs/handle/123456789/7267",
abstract = "Ispitana je inhibicija slobodne acetilholinesteraze (AChE) odabranim organotiofosfatima (OP) (diazinon, malation, hlorpirifos, azinfos-metil, forat) i njihovim oksoanalozima (diazokson, malaokson, hlorpirifos-okson, azinfos-metil-okson, forat-okson), pri čemu su optimizovani uslovi za detekciju najniže koncentracije tih jedinjenja primenom AChE testa. Određene su IC50 vrednosti za sva navedena jedinjenja. U cilju povećanja osetljivosti AChE testa, ispitivani organo-tiofosfati prevedeni su u okso-analoge u prisustvu enzima mijeloperoksidaze (MPO). To je potvreno pomoćuUPLC i GC/MS analize. Nastali oksidacioni proizvodi stabilni su najmanje 1h. Maksimalne koncentracije oksona dobijaju se kada se organo-tiofosfati inkubiraju sa 100 nM MPO u prisustvu H2O2 koncentracije 50 μM, pri pH 6, na temperaturi 25 °C u toku 10 minuta. Oksidacija OP u prisustvu MPO pod navedenim uslovima primenjena je u modifikaciji metode za detekciju OP na bazi inhibicije AChE. Određena je granica detekcije modifikovane metode, kao i efikasnost oksidacije i njena primena na smešu OP. Gore navedeni organo-tiofosfati prevedeni su u odgovarajue oksone i pomoću elektrohemijski generisanih halogena. Proizvodi koji nastaju identifikovani su kao oksoni pomoću UPLC analize. Utvrđeno je da je najefikasnija oksidacija elektrogenerisanim bromom u toku 15 minuta. Određena je i granica detekcije AChE testa za odreivanje OP kada su OP oksidovani elektrohemijski generisanim bromom..., The inhibition of acetylcholinesterase in the presence of selected organothiophosphates (diazinon, malathion, chlorpyrifos, azinphos-methyl, phorate) andtheir oxo-analogues (diazoxon, malaoxon, chlorpyrifos-oxon, azinphos-methyl-oxon,phorate-oxon) was examined. Experimental conditions were optimized to detect the lowestposible concentrations of these compounds using AChE test. IC50 values were determinedfor all the mentioned compounds.Investigated organothiophosphates have been converted into their oxo-analogues inthe presence of the enzyme myeloperoxidase. Oxidation products were detected usingUPLC and GC/MS analysis. It was found that the products are stable within minimum 1h.In order to optimize oxidation procedure and achieve maximum concentrations of oxons,the optimal concentrations of H2O2 (50 μM) and MPO (100 nM) were determined. Also,the optimal incubation time of OPs and MPO (10 min), as well as the optimal pH (6) andtemperature (25 °C) were estimated. Oxidation of OPs in the presence of MPO underoptimal conditions was applied as a modification of the method for detecting OP usingAChE test. The detection limits were determined for the modified method, as well as theefficiency of oxidation and its application for the analysi of sintetic mixture of OPs.Examined organothiophosphates have been converted into their oxo-forms usingelectrochemically generated halogens. Products have been identified as oxons using UPLCanalysis. It was found that the the most efficient oxidation of OPs was carried out by after15 minutes of oxidation with electrogenerated bromine. Detection limits were determined,too.",
publisher = "Универзитет у Београду, Хемијски факултет",
journal = "Универзитет у Београду",
title = "Primena oksidacije organo-tiofosfatnih pesticida u metodama za njihovu detekciju na bazi inhibicije acetilholinesteraze, Application of organothiophosphate pesticides oxidation in methods for their detection based on acetylcholinesterase inhibition"
}
Lazarević-Pašti, T. (2012). Application of organothiophosphate pesticides oxidation in methods for their detection based on acetylcholinesterase inhibition.
Универзитет у Београду
Универзитет у Београду, Хемијски факултет..
Lazarević-Pašti T. Application of organothiophosphate pesticides oxidation in methods for their detection based on acetylcholinesterase inhibition. Универзитет у Београду. 2012;
Lazarević-Pašti Tamara, "Application of organothiophosphate pesticides oxidation in methods for their detection based on acetylcholinesterase inhibition" Универзитет у Београду (2012)

Indirect electrochemical oxidation of organophosphorous pesticides for efficient detection via acetylcholinesterase test

Lazarević-Pašti, Tamara; Bondžić, Aleksandra; Pašti, Igor A.; Vasić, Vesna M.

(2012)

TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Bondžić, Aleksandra
AU  - Pašti, Igor A.
AU  - Vasić, Vesna M.
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5263
AB  - Organothiophosphorous pesticides diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate, have been indirectly electrochemically oxidized in aqueous media using anodically evolved Cl-2, Br-2 or I-2 as a pre-step for their detection via acetylcholinesterase-based test. The presence of single oxidation product, corresponding oxo-form, was confirmed by UPLC analysis, as well as its stability with respect to hydrolysis. Comparing different halogens, the best results were obtained using Br-2 as the oxidant due to high reactivity of HOBr, which is formed upon chemical reaction of anodically formed Br-2 with water. Limits of detection of five analyzed pesticides were lowered upon indirect electrochemical oxidation with Br-2 for two orders of magnitude or more, comparing to unoxidized parental thio-forms. In fact, the lowest possible detection limits for all five pesticides using proposed analytical procedure were achieved, as being determined by detection limits of corresponding oxo forms. Comparison of here proposed electrochemical oxidation pre-step with earlier reported ones is provided and discussed. (c) 2012 Elsevier Inc. All rights reserved.
T2  - Pesticide Biochemistry and Physiology
T1  - Indirect electrochemical oxidation of organophosphorous pesticides for efficient detection via acetylcholinesterase test
VL  - 104
IS  - 3
SP  - 236
EP  - 242
DO  - 10.1016/j.pestbp.2012.09.004
ER  - 
@article{
author = "Lazarević-Pašti, Tamara and Bondžić, Aleksandra and Pašti, Igor A. and Vasić, Vesna M.",
year = "2012",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5263",
abstract = "Organothiophosphorous pesticides diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate, have been indirectly electrochemically oxidized in aqueous media using anodically evolved Cl-2, Br-2 or I-2 as a pre-step for their detection via acetylcholinesterase-based test. The presence of single oxidation product, corresponding oxo-form, was confirmed by UPLC analysis, as well as its stability with respect to hydrolysis. Comparing different halogens, the best results were obtained using Br-2 as the oxidant due to high reactivity of HOBr, which is formed upon chemical reaction of anodically formed Br-2 with water. Limits of detection of five analyzed pesticides were lowered upon indirect electrochemical oxidation with Br-2 for two orders of magnitude or more, comparing to unoxidized parental thio-forms. In fact, the lowest possible detection limits for all five pesticides using proposed analytical procedure were achieved, as being determined by detection limits of corresponding oxo forms. Comparison of here proposed electrochemical oxidation pre-step with earlier reported ones is provided and discussed. (c) 2012 Elsevier Inc. All rights reserved.",
journal = "Pesticide Biochemistry and Physiology",
title = "Indirect electrochemical oxidation of organophosphorous pesticides for efficient detection via acetylcholinesterase test",
volume = "104",
number = "3",
pages = "236-242",
doi = "10.1016/j.pestbp.2012.09.004"
}
Lazarević-Pašti, T., Bondžić, A., Pašti, I. A.,& Vasić, V. M. (2012). Indirect electrochemical oxidation of organophosphorous pesticides for efficient detection via acetylcholinesterase test.
Pesticide Biochemistry and Physiology, 104(3), 236-242.
https://doi.org/10.1016/j.pestbp.2012.09.004
Lazarević-Pašti T, Bondžić A, Pašti IA, Vasić VM. Indirect electrochemical oxidation of organophosphorous pesticides for efficient detection via acetylcholinesterase test. Pesticide Biochemistry and Physiology. 2012;104(3):236-242
Lazarević-Pašti Tamara, Bondžić Aleksandra, Pašti Igor A., Vasić Vesna M., "Indirect electrochemical oxidation of organophosphorous pesticides for efficient detection via acetylcholinesterase test" Pesticide Biochemistry and Physiology, 104, no. 3 (2012):236-242,
https://doi.org/10.1016/j.pestbp.2012.09.004 .
13
12
15

Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts

Nastasijević, Branislav J.; Lazarević-Pašti, Tamara; Dimitrijević-Branković, Suzana I.; Pašti, Igor A.; Vujačić, Ana V.; Joksić, Gordana; Vasić, Vesna M.

(2012)

TY  - JOUR
AU  - Nastasijević, Branislav J.
AU  - Lazarević-Pašti, Tamara
AU  - Dimitrijević-Branković, Suzana I.
AU  - Pašti, Igor A.
AU  - Vujačić, Ana V.
AU  - Joksić, Gordana
AU  - Vasić, Vesna M.
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4873
AB  - The aim of this study was to investigate the inhibitory activity of Gentiana lutea extracts on the enzyme myeloperoxidase (MPO), as well as the antioxidant activity of these extracts and their correlation with the total polyphenol content. Extracts were prepared using methanol (100%), water and ethanol aqueous solutions (96, 75, 50 and 25% v/v) as solvents for extraction. Also, isovitexin, amarogentin and gentiopicroside, pharmacologically active constituents of G. lutea were tested as potential inhibitors of MPO. Antioxidant activity of extracts was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging test and also using cyclic voltammetry (CV). Among all extracts, the antioxidant capacity of 50% ethanol aqueous extract was the highest, both when measured using the DPPH test, with IC50 = 20.6 mu g/ml, and when using CV. Also, 50% ethanol extract, showed the best inhibition of MPO activity in comparison with other extracts. In the group of the selected G. lutea constituents, gentiopicroside has proved to be the strongest inhibitor of MPO, with IC50 = 0.8 mu g/ml. Also, the concentration of G. lutea constituents were determined in all extracts, using Ultra Performance Liquid Chromatography (UPLC). (C) 2012 Elsevier B.V. All rights reserved.
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts
VL  - 66
SP  - 191
EP  - 196
DO  - 10.1016/j.jpba.2012.03.052
ER  - 
@article{
author = "Nastasijević, Branislav J. and Lazarević-Pašti, Tamara and Dimitrijević-Branković, Suzana I. and Pašti, Igor A. and Vujačić, Ana V. and Joksić, Gordana and Vasić, Vesna M.",
year = "2012",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4873",
abstract = "The aim of this study was to investigate the inhibitory activity of Gentiana lutea extracts on the enzyme myeloperoxidase (MPO), as well as the antioxidant activity of these extracts and their correlation with the total polyphenol content. Extracts were prepared using methanol (100%), water and ethanol aqueous solutions (96, 75, 50 and 25% v/v) as solvents for extraction. Also, isovitexin, amarogentin and gentiopicroside, pharmacologically active constituents of G. lutea were tested as potential inhibitors of MPO. Antioxidant activity of extracts was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging test and also using cyclic voltammetry (CV). Among all extracts, the antioxidant capacity of 50% ethanol aqueous extract was the highest, both when measured using the DPPH test, with IC50 = 20.6 mu g/ml, and when using CV. Also, 50% ethanol extract, showed the best inhibition of MPO activity in comparison with other extracts. In the group of the selected G. lutea constituents, gentiopicroside has proved to be the strongest inhibitor of MPO, with IC50 = 0.8 mu g/ml. Also, the concentration of G. lutea constituents were determined in all extracts, using Ultra Performance Liquid Chromatography (UPLC). (C) 2012 Elsevier B.V. All rights reserved.",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts",
volume = "66",
pages = "191-196",
doi = "10.1016/j.jpba.2012.03.052"
}
Nastasijević, B. J., Lazarević-Pašti, T., Dimitrijević-Branković, S. I., Pašti, I. A., Vujačić, A. V., Joksić, G.,& Vasić, V. M. (2012). Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts.
Journal of Pharmaceutical and Biomedical Analysis, 66, 191-196.
https://doi.org/10.1016/j.jpba.2012.03.052
Nastasijević BJ, Lazarević-Pašti T, Dimitrijević-Branković SI, Pašti IA, Vujačić AV, Joksić G, Vasić VM. Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts. Journal of Pharmaceutical and Biomedical Analysis. 2012;66:191-196
Nastasijević Branislav J., Lazarević-Pašti Tamara, Dimitrijević-Branković Suzana I., Pašti Igor A., Vujačić Ana V., Joksić Gordana, Vasić Vesna M., "Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts" Journal of Pharmaceutical and Biomedical Analysis, 66 (2012):191-196,
https://doi.org/10.1016/j.jpba.2012.03.052 .
47
44
50

Oxidation of diazinon and malathion by myeloperoxidase

Lazarević-Pašti, Tamara; Čolović, Mirjana B.; Savić, Jasmina; Momić, Tatjana; Vasić, Vesna M.

(2011)

TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Čolović, Mirjana B.
AU  - Savić, Jasmina
AU  - Momić, Tatjana
AU  - Vasić, Vesna M.
PY  - 2011
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4332
AB  - The aim of the work was to investigate the in vitro oxidation of diazinon and malathion, organophosphorous pesticides (OPs) containing phosphorthioate group, catalyzed by enzyme myeloperoxidase (MPO). The oxidation was performed in the presence of hydrogen peroxide. The products were identified as oxon derivatives (phosphates), where the sulfur atom from thioate group was substituted by an oxygen atom. No hydrolysis products were detected after enzyme - induced oxidation. The oxidation efficiency was controlled using acethylcholinesterase (AChE) bioassay for determination of oxon derivatives concentration. The influence of OPs concentration, incubation time of OPs with MPO, as well as MPO concentration on the yield of oxo forms was investigated. Kinetic constants of MPO in oxidation of malathion and diazinon were estimated. The maximum concentration of oxo forms was achieved after 10 min incubation of OPs in 50 mM phosphate buffer (pH 6.0) with 100 nM MPO. (C) 2011 Elsevier Inc. All rights reserved.
T2  - Pesticide Biochemistry and Physiology
T1  - Oxidation of diazinon and malathion by myeloperoxidase
VL  - 100
IS  - 2
SP  - 140
EP  - 144
DO  - 10.1016/j.pestbp.2011.03.001
ER  - 
@article{
author = "Lazarević-Pašti, Tamara and Čolović, Mirjana B. and Savić, Jasmina and Momić, Tatjana and Vasić, Vesna M.",
year = "2011",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4332",
abstract = "The aim of the work was to investigate the in vitro oxidation of diazinon and malathion, organophosphorous pesticides (OPs) containing phosphorthioate group, catalyzed by enzyme myeloperoxidase (MPO). The oxidation was performed in the presence of hydrogen peroxide. The products were identified as oxon derivatives (phosphates), where the sulfur atom from thioate group was substituted by an oxygen atom. No hydrolysis products were detected after enzyme - induced oxidation. The oxidation efficiency was controlled using acethylcholinesterase (AChE) bioassay for determination of oxon derivatives concentration. The influence of OPs concentration, incubation time of OPs with MPO, as well as MPO concentration on the yield of oxo forms was investigated. Kinetic constants of MPO in oxidation of malathion and diazinon were estimated. The maximum concentration of oxo forms was achieved after 10 min incubation of OPs in 50 mM phosphate buffer (pH 6.0) with 100 nM MPO. (C) 2011 Elsevier Inc. All rights reserved.",
journal = "Pesticide Biochemistry and Physiology",
title = "Oxidation of diazinon and malathion by myeloperoxidase",
volume = "100",
number = "2",
pages = "140-144",
doi = "10.1016/j.pestbp.2011.03.001"
}
Lazarević-Pašti, T., Čolović, M. B., Savić, J., Momić, T.,& Vasić, V. M. (2011). Oxidation of diazinon and malathion by myeloperoxidase.
Pesticide Biochemistry and Physiology, 100(2), 140-144.
https://doi.org/10.1016/j.pestbp.2011.03.001
Lazarević-Pašti T, Čolović MB, Savić J, Momić T, Vasić VM. Oxidation of diazinon and malathion by myeloperoxidase. Pesticide Biochemistry and Physiology. 2011;100(2):140-144
Lazarević-Pašti Tamara, Čolović Mirjana B., Savić Jasmina, Momić Tatjana, Vasić Vesna M., "Oxidation of diazinon and malathion by myeloperoxidase" Pesticide Biochemistry and Physiology, 100, no. 2 (2011):140-144,
https://doi.org/10.1016/j.pestbp.2011.03.001 .
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Oxidation of Diazinon for the Sensitive Detection By Cholinesterase-Based Bioanalytical Method

Lazarević-Pašti, Tamara; Vasić, Vesna M.

(2011)

TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Vasić, Vesna M.
PY  - 2011
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4534
AB  - The conversion of diazinon, the model compound for OPs, to more potent AChE inhibitor diazoxon was investigated, using enzyme myeloperoxidase (MPO) as an oxidant. The aim was to enhance its inhibitory power in order to develop rapid, effective and specific acethylcholinesterase (AChE)-based bioanalytical method for its detection in environment. 5-min incubation of 1 x 10(-5) M - 1 x 10(-7) M diazinon with 100 nM MPO increased the degree of AChE inhibition from 80 to 10% due to the formation of diazoxon, compared to the control values for samples containing the same diazinon concentrations in untreated samples. The calibration graph for diazinon detection with AChE assay after incubation with MPO was constructed. While the lower detection limit of diazinon (10% AChE inhibition) before oxidation was ca. 1 x 10(-5) M, the detection limit after oxidation was below 1 x 10(-8) M.
T2  - Journal of Environmental Protection and Ecology
T1  - Oxidation of Diazinon for the Sensitive Detection By Cholinesterase-Based Bioanalytical Method
VL  - 12
IS  - 3
SP  - 1168
EP  - 1173
ER  - 
@article{
author = "Lazarević-Pašti, Tamara and Vasić, Vesna M.",
year = "2011",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4534",
abstract = "The conversion of diazinon, the model compound for OPs, to more potent AChE inhibitor diazoxon was investigated, using enzyme myeloperoxidase (MPO) as an oxidant. The aim was to enhance its inhibitory power in order to develop rapid, effective and specific acethylcholinesterase (AChE)-based bioanalytical method for its detection in environment. 5-min incubation of 1 x 10(-5) M - 1 x 10(-7) M diazinon with 100 nM MPO increased the degree of AChE inhibition from 80 to 10% due to the formation of diazoxon, compared to the control values for samples containing the same diazinon concentrations in untreated samples. The calibration graph for diazinon detection with AChE assay after incubation with MPO was constructed. While the lower detection limit of diazinon (10% AChE inhibition) before oxidation was ca. 1 x 10(-5) M, the detection limit after oxidation was below 1 x 10(-8) M.",
journal = "Journal of Environmental Protection and Ecology",
title = "Oxidation of Diazinon for the Sensitive Detection By Cholinesterase-Based Bioanalytical Method",
volume = "12",
number = "3",
pages = "1168-1173"
}
Lazarević-Pašti, T.,& Vasić, V. M. (2011). Oxidation of Diazinon for the Sensitive Detection By Cholinesterase-Based Bioanalytical Method.
Journal of Environmental Protection and Ecology, 12(3), 1168-1173.
Lazarević-Pašti T, Vasić VM. Oxidation of Diazinon for the Sensitive Detection By Cholinesterase-Based Bioanalytical Method. Journal of Environmental Protection and Ecology. 2011;12(3):1168-1173
Lazarević-Pašti Tamara, Vasić Vesna M., "Oxidation of Diazinon for the Sensitive Detection By Cholinesterase-Based Bioanalytical Method" Journal of Environmental Protection and Ecology, 12, no. 3 (2011):1168-1173
3

Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase

Lazarević-Pašti, Tamara; Nastasijević, Branislav J.; Vasić, Vesna M.

(2011)

TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Nastasijević, Branislav J.
AU  - Vasić, Vesna M.
PY  - 2011
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4597
AB  - The present paper deals with the investigations of optimal conditions for the myeloperoxidase (MPO) mediated oxidation of chlorpyrifos, azinphos-methyl and phorate, organophosphorous pesticides (OPs) containing phosphorothionate group, from thio- to oxo-forms, in the presence of hydrogen peroxide. The aim of the work was to apply this oxidation method in the AChE based bioanalytical tests for OPs determination. The maximum concentration of oxo-forms for all tested pesticides was achieved after 10 min incubation of OPs in 50 mM phosphate buffer (pH 6.0) with 100 nM MPO in the presence of 50 mu M H(2)O(2). Optimal temperature for obtaining maximal concentration of oxo-forms was 37 degrees C. Only the parent compounds and their oxo-forms were identified chromatographically in the OPs samples after their exposure to MPO. Moreover, no hydrolysis products were detected in the time interval of 1 h after the MPO catalyzed reaction was stopped by catalase. The efficiency of OPs transformation from thio- to oxo-forms was measured using acethylcholinesterase (AChE) test, by comparison of percent of AChE inhibition before and after exposure to the oxidized sample. (C) 2011 Elsevier Inc. All rights reserved.
T2  - Pesticide Biochemistry and Physiology
T1  - Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase
VL  - 101
IS  - 3
SP  - 220
EP  - 226
DO  - 10.1016/j.pestbp.2011.09.009
ER  - 
@article{
author = "Lazarević-Pašti, Tamara and Nastasijević, Branislav J. and Vasić, Vesna M.",
year = "2011",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4597",
abstract = "The present paper deals with the investigations of optimal conditions for the myeloperoxidase (MPO) mediated oxidation of chlorpyrifos, azinphos-methyl and phorate, organophosphorous pesticides (OPs) containing phosphorothionate group, from thio- to oxo-forms, in the presence of hydrogen peroxide. The aim of the work was to apply this oxidation method in the AChE based bioanalytical tests for OPs determination. The maximum concentration of oxo-forms for all tested pesticides was achieved after 10 min incubation of OPs in 50 mM phosphate buffer (pH 6.0) with 100 nM MPO in the presence of 50 mu M H(2)O(2). Optimal temperature for obtaining maximal concentration of oxo-forms was 37 degrees C. Only the parent compounds and their oxo-forms were identified chromatographically in the OPs samples after their exposure to MPO. Moreover, no hydrolysis products were detected in the time interval of 1 h after the MPO catalyzed reaction was stopped by catalase. The efficiency of OPs transformation from thio- to oxo-forms was measured using acethylcholinesterase (AChE) test, by comparison of percent of AChE inhibition before and after exposure to the oxidized sample. (C) 2011 Elsevier Inc. All rights reserved.",
journal = "Pesticide Biochemistry and Physiology",
title = "Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase",
volume = "101",
number = "3",
pages = "220-226",
doi = "10.1016/j.pestbp.2011.09.009"
}
Lazarević-Pašti, T., Nastasijević, B. J.,& Vasić, V. M. (2011). Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase.
Pesticide Biochemistry and Physiology, 101(3), 220-226.
https://doi.org/10.1016/j.pestbp.2011.09.009
Lazarević-Pašti T, Nastasijević BJ, Vasić VM. Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase. Pesticide Biochemistry and Physiology. 2011;101(3):220-226
Lazarević-Pašti Tamara, Nastasijević Branislav J., Vasić Vesna M., "Oxidation of chlorpyrifos, azinphos-methyl and phorate by myeloperoxidase" Pesticide Biochemistry and Physiology, 101, no. 3 (2011):220-226,
https://doi.org/10.1016/j.pestbp.2011.09.009 .
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