TY  - JOUR
AU  - Tanić, Nikola
AU  - Vujošević, Mladen
AU  - Dedović-Tanić, Nasta
AU  - Dimitrijević, Bogomir B.
PY  - 2005
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2866
AB  - Most B chromosomes are heavily heterochromatic, promoting the general idea that they are genetically inert. The B chromosomes of Apodemus flavicollis are euchromatic and show a high degree of homology with the chromosomes. The euchromatic nature of chromosomes in A. flavicollis suggests that they may carry active genes and have transcriptional activity. We applied the differential display reverse transcription-polymerase chain reaction (DD RT-PCR) in order to analyze and compare gene expression in animals possessing chromosomes and animals without B chromosomes. After a second and third round of amplification, three cDNA fragments were differentially expressed in +B mice compared with 0B animals. These cDNAs were Chaperonin containing TCP-1, subunit 6b (zeta) (CCT6B), Fragile histidine triad gene (FHIT) and hypothetical gene XP transcript. The differential expression pattern was confirmed by Real Time RT-PCR. We suggest that altered expression of these important genes is due to the presence of B chromosomes. In elevating the expression of these genes, B chromosomes of A. flavicollis affect some of the crucial processes in the cell. The significance of these effects and the nature of B chromosomes of A. flavicollis are discussed in the context of the data presented.
T2  - Chromosoma
T1  - Differential gene expression in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia) with and without B chromosomes
VL  - 113
IS  - 8
SP  - 418
EP  - 427
DO  - 10.1007/s00412-004-0327-z
ER  - 

@article{
author = "Tanić, Nikola and Vujošević, Mladen and Dedović-Tanić, Nasta and Dimitrijević, Bogomir B.",
year = "2005",
abstract = "Most B chromosomes are heavily heterochromatic, promoting the general idea that they are genetically inert. The B chromosomes of Apodemus flavicollis are euchromatic and show a high degree of homology with the chromosomes. The euchromatic nature of chromosomes in A. flavicollis suggests that they may carry active genes and have transcriptional activity. We applied the differential display reverse transcription-polymerase chain reaction (DD RT-PCR) in order to analyze and compare gene expression in animals possessing chromosomes and animals without B chromosomes. After a second and third round of amplification, three cDNA fragments were differentially expressed in +B mice compared with 0B animals. These cDNAs were Chaperonin containing TCP-1, subunit 6b (zeta) (CCT6B), Fragile histidine triad gene (FHIT) and hypothetical gene XP transcript. The differential expression pattern was confirmed by Real Time RT-PCR. We suggest that altered expression of these important genes is due to the presence of B chromosomes. In elevating the expression of these genes, B chromosomes of A. flavicollis affect some of the crucial processes in the cell. The significance of these effects and the nature of B chromosomes of A. flavicollis are discussed in the context of the data presented.",
journal = "Chromosoma",
title = "Differential gene expression in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia) with and without B chromosomes",
volume = "113",
number = "8",
pages = "418-427",
doi = "10.1007/s00412-004-0327-z"
}

Tanić, N., Vujošević, M., Dedović-Tanić, N.,& Dimitrijević, B. B.. (2005). Differential gene expression in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia) with and without B chromosomes. in Chromosoma, 113(8), 418-427.
https://doi.org/10.1007/s00412-004-0327-z

Tanić N, Vujošević M, Dedović-Tanić N, Dimitrijević BB. Differential gene expression in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia) with and without B chromosomes. in Chromosoma. 2005;113(8):418-427.
doi:10.1007/s00412-004-0327-z .

Tanić, Nikola, Vujošević, Mladen, Dedović-Tanić, Nasta, Dimitrijević, Bogomir B., "Differential gene expression in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia) with and without B chromosomes" in Chromosoma, 113, no. 8 (2005):418-427,
https://doi.org/10.1007/s00412-004-0327-z . .

TY  - JOUR
AU  - Tanić, Nikola
AU  - Dedović-Tanić, Nasta
AU  - Vujošević, Mladen
AU  - Dimitrijević, Bogomir B.
PY  - 2000
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2325
AB  - Using AP-PCR-based DNA profiling we examined some structural features of B chromosomes from yellow-necked mice Apodemus flavicollis. Mice harboring one,two, or three or lacking B chromosomes were examined. Chromosomal structure was scanned for variant bands by using a series of arbitrary primers and From these, informative bands were selected. The selection:criteria used were the ability to differentiate between individuals of the species, to detect markers common For both A and B chromosomes, and, importantly, to differentiate between A- and B-chromosome sets. In addition to primers, profiling conditions were found to be critical for meeting the selection criteria. Primers and analysis conditions that demonstrated structural characteristics unique to the B-chromosome set are described. These characteristics included variant bands as qualitative parameters and altered electrophoretic band-intensities as quantitative distinctions estimated by integration of densitometric profiles of electrophoretograms. B chromosome-specific molecular markers are easy to detect by AP-PCR-based DNA profiling in the presence of a full set of A chromosomes. Models for the origin of yellow-necked mouse B chromosomes are discussed in the context of presented data.
T2  - Genome Research
T1  - DNA profiling of B chromosomes from the yellow-necked mouse Apodemus flavicollis (Rodentia, Mammalia)
VL  - 10
IS  - 1
SP  - 55
EP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2325
ER  - 

@article{
author = "Tanić, Nikola and Dedović-Tanić, Nasta and Vujošević, Mladen and Dimitrijević, Bogomir B.",
year = "2000",
abstract = "Using AP-PCR-based DNA profiling we examined some structural features of B chromosomes from yellow-necked mice Apodemus flavicollis. Mice harboring one,two, or three or lacking B chromosomes were examined. Chromosomal structure was scanned for variant bands by using a series of arbitrary primers and From these, informative bands were selected. The selection:criteria used were the ability to differentiate between individuals of the species, to detect markers common For both A and B chromosomes, and, importantly, to differentiate between A- and B-chromosome sets. In addition to primers, profiling conditions were found to be critical for meeting the selection criteria. Primers and analysis conditions that demonstrated structural characteristics unique to the B-chromosome set are described. These characteristics included variant bands as qualitative parameters and altered electrophoretic band-intensities as quantitative distinctions estimated by integration of densitometric profiles of electrophoretograms. B chromosome-specific molecular markers are easy to detect by AP-PCR-based DNA profiling in the presence of a full set of A chromosomes. Models for the origin of yellow-necked mouse B chromosomes are discussed in the context of presented data.",
journal = "Genome Research",
title = "DNA profiling of B chromosomes from the yellow-necked mouse Apodemus flavicollis (Rodentia, Mammalia)",
volume = "10",
number = "1",
pages = "55-61",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2325"
}

Tanić, N., Dedović-Tanić, N., Vujošević, M.,& Dimitrijević, B. B.. (2000). DNA profiling of B chromosomes from the yellow-necked mouse Apodemus flavicollis (Rodentia, Mammalia). in Genome Research, 10(1), 55-61.
https://hdl.handle.net/21.15107/rcub_vinar_2325

Tanić N, Dedović-Tanić N, Vujošević M, Dimitrijević BB. DNA profiling of B chromosomes from the yellow-necked mouse Apodemus flavicollis (Rodentia, Mammalia). in Genome Research. 2000;10(1):55-61.
https://hdl.handle.net/21.15107/rcub_vinar_2325 .

Tanić, Nikola, Dedović-Tanić, Nasta, Vujošević, Mladen, Dimitrijević, Bogomir B., "DNA profiling of B chromosomes from the yellow-necked mouse Apodemus flavicollis (Rodentia, Mammalia)" in Genome Research, 10, no. 1 (2000):55-61,
https://hdl.handle.net/21.15107/rcub_vinar_2325 .