TY  - CONF
AU  - Blagojević, Danijela
AU  - Milanović, Ivana
AU  - Imširović, Mirela
AU  - Lazić, Sanda
AU  - Maksimović, Zlatko
AU  - Tanić, Nasta
AU  - Tanić, Nikola
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13316
AB  - COVID-19 pandemic caused by coronavirus 2 respiratory virus (SARS-CoV-2) required fast and effective diagnostics for the purpose of preventing the spread of the disease and outbreak monitoring and control. Therefore, prompt, accurate and reliable testing was of particular importance. Indubitably, the most representative, the most sensitive and the fastest method for detection of SARS CoV-2 is Real Time RT-PCR (RT-qPCR). During the pandemic there were numerous different assays, reagents, protocols and reporting procedures under the question mark because of lack of certified standards, standardization of RNA extraction and reporting procedures. In practice, the reliability of RT-qPCR results depend on a number of parameters that include sample collection and processing, method of RNA extraction, choice of assay, choice of instrument, analysis method and operator intervention. Here we present the various challenges which we encountered during our work and emphasize comparative analysis by different assays, as well as automated versus manual RNA extraction. Sampling, adequate manipulation load, the quality of the assay and interpretation of the result are extremely important. Our results revealed that manual viral RNA extraction should be a method of choice for high sensitivity. In addition, amplification assays targeting three SARS-CoV-2 genes are much more efficient from those targeting one. Unfortunately, RT-qPCR (originally quantitative method) was exclusively used as qualitative diagnostic test for SARS-CoV-2. We think that the ideal testing regimen would involve not just qualitative detection of SARS-CoV-2 but reliable and meaningful quantitative reporting of viral load. For such a thing, a consensus at the level of the world medical community is necessary regarding the most important thing: defining the cut-off value for a clinically significant viral load. It has never been done and therefore many people, all over the world, were sentenced to house arrest for no reason at all.
C3  - Genetics & Applications
T1  - Challenges in SARS-COV-2 diagnostics by real time RT-PCR
SP  - 33
EP  - 33
ER  - 

@conference{
author = "Blagojević, Danijela and Milanović, Ivana and Imširović, Mirela and Lazić, Sanda and Maksimović, Zlatko and Tanić, Nasta and Tanić, Nikola",
year = "2024",
abstract = "COVID-19 pandemic caused by coronavirus 2 respiratory virus (SARS-CoV-2) required fast and effective diagnostics for the purpose of preventing the spread of the disease and outbreak monitoring and control. Therefore, prompt, accurate and reliable testing was of particular importance. Indubitably, the most representative, the most sensitive and the fastest method for detection of SARS CoV-2 is Real Time RT-PCR (RT-qPCR). During the pandemic there were numerous different assays, reagents, protocols and reporting procedures under the question mark because of lack of certified standards, standardization of RNA extraction and reporting procedures. In practice, the reliability of RT-qPCR results depend on a number of parameters that include sample collection and processing, method of RNA extraction, choice of assay, choice of instrument, analysis method and operator intervention. Here we present the various challenges which we encountered during our work and emphasize comparative analysis by different assays, as well as automated versus manual RNA extraction. Sampling, adequate manipulation load, the quality of the assay and interpretation of the result are extremely important. Our results revealed that manual viral RNA extraction should be a method of choice for high sensitivity. In addition, amplification assays targeting three SARS-CoV-2 genes are much more efficient from those targeting one. Unfortunately, RT-qPCR (originally quantitative method) was exclusively used as qualitative diagnostic test for SARS-CoV-2. We think that the ideal testing regimen would involve not just qualitative detection of SARS-CoV-2 but reliable and meaningful quantitative reporting of viral load. For such a thing, a consensus at the level of the world medical community is necessary regarding the most important thing: defining the cut-off value for a clinically significant viral load. It has never been done and therefore many people, all over the world, were sentenced to house arrest for no reason at all.",
journal = "Genetics & Applications",
title = "Challenges in SARS-COV-2 diagnostics by real time RT-PCR",
pages = "33-33"
}

Blagojević, D., Milanović, I., Imširović, M., Lazić, S., Maksimović, Z., Tanić, N.,& Tanić, N.. (2024). Challenges in SARS-COV-2 diagnostics by real time RT-PCR. in Genetics & Applications, 33-33.

Blagojević D, Milanović I, Imširović M, Lazić S, Maksimović Z, Tanić N, Tanić N. Challenges in SARS-COV-2 diagnostics by real time RT-PCR. in Genetics & Applications. 2024;:33-33..

Blagojević, Danijela, Milanović, Ivana, Imširović, Mirela, Lazić, Sanda, Maksimović, Zlatko, Tanić, Nasta, Tanić, Nikola, "Challenges in SARS-COV-2 diagnostics by real time RT-PCR" in Genetics & Applications (2024):33-33.

TY  - JOUR
AU  - Prvanović, Mirjana
AU  - Nedeljković, Milica
AU  - Tanić, Nasta
AU  - Tomić, Tijana
AU  - Terzić, Tanja
AU  - Milovanović, Zorka
AU  - Maksimović, Zlatko
AU  - Tanić, Nikola
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10064
AB  - Breast cancer is the most commonly occurring malignancy and the leading cause of cancer-related death in women. Triple-negative breast cancer (TNBC) is the most aggressive subtype and is associated with high recurrence rates, high incidence of distant metastases, and poor overall survival. The aim of this study was to investigate the PI3K/PTEN/Akt/mTOR pathway as one of the most frequently deregulated pathways in cancer. We aimed to explore the impact of PI3K and mTOR oncogenes as well as the PTEN tumor suppressor on TNBC clinical behavior, prognosis, and multidrug resistance (MDR), using immunohistochemistry and copy number analysis by quantitative real-time PCR. Our results revealed that loss of PTEN and high expression of PI3K and mTOR proteins are associated with poor outcome of TNBC patients. PTEN deletions appeared as a major cause of reduced or absent PTEN expression in TNBC. Importantly, homozygous deletions of PTEN (and not hemizygous deletions) are a potential molecular marker of metastasis formation and good predictors of TNBC outcome. In conclusion, we believe that concurrent examination of PTEN/PI3K/mTOR protein expression may be more useful in predicting TNBC clinical course than the analysis of single protein expression. Specifically, our results showed that PTEN-reduced/PI3K-high/mTOR-high expression constitutes a 'high risk' profile of TNBC.
T2  - Life (Basel, Switzerland)
T1  - Role of PTEN, PI3K, and mTOR in Triple-Negative Breast Cancer.
VL  - 11
IS  - 11
SP  - 1247
DO  - 10.3390/life11111247
ER  - 

@article{
author = "Prvanović, Mirjana and Nedeljković, Milica and Tanić, Nasta and Tomić, Tijana and Terzić, Tanja and Milovanović, Zorka and Maksimović, Zlatko and Tanić, Nikola",
year = "2021",
abstract = "Breast cancer is the most commonly occurring malignancy and the leading cause of cancer-related death in women. Triple-negative breast cancer (TNBC) is the most aggressive subtype and is associated with high recurrence rates, high incidence of distant metastases, and poor overall survival. The aim of this study was to investigate the PI3K/PTEN/Akt/mTOR pathway as one of the most frequently deregulated pathways in cancer. We aimed to explore the impact of PI3K and mTOR oncogenes as well as the PTEN tumor suppressor on TNBC clinical behavior, prognosis, and multidrug resistance (MDR), using immunohistochemistry and copy number analysis by quantitative real-time PCR. Our results revealed that loss of PTEN and high expression of PI3K and mTOR proteins are associated with poor outcome of TNBC patients. PTEN deletions appeared as a major cause of reduced or absent PTEN expression in TNBC. Importantly, homozygous deletions of PTEN (and not hemizygous deletions) are a potential molecular marker of metastasis formation and good predictors of TNBC outcome. In conclusion, we believe that concurrent examination of PTEN/PI3K/mTOR protein expression may be more useful in predicting TNBC clinical course than the analysis of single protein expression. Specifically, our results showed that PTEN-reduced/PI3K-high/mTOR-high expression constitutes a 'high risk' profile of TNBC.",
journal = "Life (Basel, Switzerland)",
title = "Role of PTEN, PI3K, and mTOR in Triple-Negative Breast Cancer.",
volume = "11",
number = "11",
pages = "1247",
doi = "10.3390/life11111247"
}

Prvanović, M., Nedeljković, M., Tanić, N., Tomić, T., Terzić, T., Milovanović, Z., Maksimović, Z.,& Tanić, N.. (2021). Role of PTEN, PI3K, and mTOR in Triple-Negative Breast Cancer.. in Life (Basel, Switzerland), 11(11), 1247.
https://doi.org/10.3390/life11111247

Prvanović M, Nedeljković M, Tanić N, Tomić T, Terzić T, Milovanović Z, Maksimović Z, Tanić N. Role of PTEN, PI3K, and mTOR in Triple-Negative Breast Cancer.. in Life (Basel, Switzerland). 2021;11(11):1247.
doi:10.3390/life11111247 .

Prvanović, Mirjana, Nedeljković, Milica, Tanić, Nasta, Tomić, Tijana, Terzić, Tanja, Milovanović, Zorka, Maksimović, Zlatko, Tanić, Nikola, "Role of PTEN, PI3K, and mTOR in Triple-Negative Breast Cancer." in Life (Basel, Switzerland), 11, no. 11 (2021):1247,
https://doi.org/10.3390/life11111247 . .