TY  - JOUR
AU  - Milojkov, Dušan V.
AU  - Silvestre, Oscar F.
AU  - Stanić, Vojislav
AU  - Janjić, Goran V.
AU  - Mutavdžić, Dragosav R.
AU  - Milanović, Marija
AU  - Nieder, Jana B.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8529
AB  - Fluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiplet states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Additionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (3P0→3H5 transition of Pr3+ ion) and orange emission at 600 nm (1D2→3H4), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications. © 2019
T2  - Journal of Luminescence
T1  - Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents
VL  - 217
SP  - 116757
DO  - 10.1016/j.jlumin.2019.116757
ER  - 

@article{
author = "Milojkov, Dušan V. and Silvestre, Oscar F. and Stanić, Vojislav and Janjić, Goran V. and Mutavdžić, Dragosav R. and Milanović, Marija and Nieder, Jana B.",
year = "2020",
abstract = "Fluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiplet states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Additionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (3P0→3H5 transition of Pr3+ ion) and orange emission at 600 nm (1D2→3H4), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications. © 2019",
journal = "Journal of Luminescence",
title = "Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents",
volume = "217",
pages = "116757",
doi = "10.1016/j.jlumin.2019.116757"
}

Milojkov, D. V., Silvestre, O. F., Stanić, V., Janjić, G. V., Mutavdžić, D. R., Milanović, M.,& Nieder, J. B.. (2020). Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents. in Journal of Luminescence, 217, 116757.
https://doi.org/10.1016/j.jlumin.2019.116757

Milojkov DV, Silvestre OF, Stanić V, Janjić GV, Mutavdžić DR, Milanović M, Nieder JB. Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents. in Journal of Luminescence. 2020;217:116757.
doi:10.1016/j.jlumin.2019.116757 .

Milojkov, Dušan V., Silvestre, Oscar F., Stanić, Vojislav, Janjić, Goran V., Mutavdžić, Dragosav R., Milanović, Marija, Nieder, Jana B., "Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents" in Journal of Luminescence, 217 (2020):116757,
https://doi.org/10.1016/j.jlumin.2019.116757 . .