TY  - JOUR
AU  - Cabrera-Barjas, Gustavo
AU  - Radovanović, Neda
AU  - Arrepol, Gaston Bravo
AU  - de la Torre, Alexander F.
AU  - Valdes, Oscar
AU  - Nešić, Aleksandra
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10700
AB  - The efficient use of waste from food processing industry is one of the innovative approaches within sustainable development, because it can be transferred into added value products, which could improve economic, energetic and environmental sectors. In this context, the squid pen waste from seafood industry was used as raw material to obtain nanofibrous beta-chitin films. In order to extend functionality of obtained films, elderberry extract obtained from biomass was added at different concentrations. The tensile strength of chitin-elderberry extract films was improved by 52%, elongation at break by 153% and water vapor barrier by 65%. The obtained material showed distinct color change when subjected to acidic or basic solutions. It was proven by CIELab color analysis that all color changes could be easily perceived visually. In addition, the obtained nanofibrous film was successfully used to monitor the freshness of Hake fish. Namely, when the film was introduced in a package that contained fresh fish, its color was efficiently changed within the time during the storage at 4 degrees C. The obtained results demonstrated that food processing waste could be efficiently valorized, and could give sustainable food package design as a spoilage indicator of high protein food.
T2  - International Journal of Biological Macromolecules
T1  - Valorization of food waste to produce intelligent nanofibrous beta-chitin films
VL  - 186
SP  - 92
EP  - 99
DO  - 10.1016/j.ijbiomac.2021.07.045
ER  - 

@article{
author = "Cabrera-Barjas, Gustavo and Radovanović, Neda and Arrepol, Gaston Bravo and de la Torre, Alexander F. and Valdes, Oscar and Nešić, Aleksandra",
year = "2021",
abstract = "The efficient use of waste from food processing industry is one of the innovative approaches within sustainable development, because it can be transferred into added value products, which could improve economic, energetic and environmental sectors. In this context, the squid pen waste from seafood industry was used as raw material to obtain nanofibrous beta-chitin films. In order to extend functionality of obtained films, elderberry extract obtained from biomass was added at different concentrations. The tensile strength of chitin-elderberry extract films was improved by 52%, elongation at break by 153% and water vapor barrier by 65%. The obtained material showed distinct color change when subjected to acidic or basic solutions. It was proven by CIELab color analysis that all color changes could be easily perceived visually. In addition, the obtained nanofibrous film was successfully used to monitor the freshness of Hake fish. Namely, when the film was introduced in a package that contained fresh fish, its color was efficiently changed within the time during the storage at 4 degrees C. The obtained results demonstrated that food processing waste could be efficiently valorized, and could give sustainable food package design as a spoilage indicator of high protein food.",
journal = "International Journal of Biological Macromolecules",
title = "Valorization of food waste to produce intelligent nanofibrous beta-chitin films",
volume = "186",
pages = "92-99",
doi = "10.1016/j.ijbiomac.2021.07.045"
}

Cabrera-Barjas, G., Radovanović, N., Arrepol, G. B., de la Torre, A. F., Valdes, O.,& Nešić, A.. (2021). Valorization of food waste to produce intelligent nanofibrous beta-chitin films. in International Journal of Biological Macromolecules, 186, 92-99.
https://doi.org/10.1016/j.ijbiomac.2021.07.045

Cabrera-Barjas G, Radovanović N, Arrepol GB, de la Torre AF, Valdes O, Nešić A. Valorization of food waste to produce intelligent nanofibrous beta-chitin films. in International Journal of Biological Macromolecules. 2021;186:92-99.
doi:10.1016/j.ijbiomac.2021.07.045 .

Cabrera-Barjas, Gustavo, Radovanović, Neda, Arrepol, Gaston Bravo, de la Torre, Alexander F., Valdes, Oscar, Nešić, Aleksandra, "Valorization of food waste to produce intelligent nanofibrous beta-chitin films" in International Journal of Biological Macromolecules, 186 (2021):92-99,
https://doi.org/10.1016/j.ijbiomac.2021.07.045 . .

TY  - JOUR
AU  - Nešić, Aleksandra
AU  - Cabrera-Barjas, Gustavo
AU  - Dimitrijević-Branković, Suzana I.
AU  - Davidović, Slađana Z.
AU  - Radovanović, Neda
AU  - Delattre, Cédric
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8800
AB  - The use of polysaccharide-based materials presents an eco-friendly technological solution, by reducing dependence on fossil resources while reducing a product’s carbon footprint, when compared to conventional plastic packaging materials. This review discusses the potential of polysaccharides as a raw material to produce multifunctional materials for food packaging applications. The covered areas include the recent innovations and properties of the polysaccharide-based materials. Emphasis is given to hemicelluloses, marine polysaccharides, and bacterial exopolysaccharides and their potential application in the latest trends of food packaging materials, including edible coatings, intelligent films, and thermo-insulated aerogel packaging.
T2  - Molecules
T1  - Prospect of Polysaccharide-Based Materials as Advanced Food Packaging
VL  - 25
IS  - 1
SP  - 135
DO  - 10.3390/molecules25010135
ER  - 

@article{
author = "Nešić, Aleksandra and Cabrera-Barjas, Gustavo and Dimitrijević-Branković, Suzana I. and Davidović, Slađana Z. and Radovanović, Neda and Delattre, Cédric",
year = "2019",
abstract = "The use of polysaccharide-based materials presents an eco-friendly technological solution, by reducing dependence on fossil resources while reducing a product’s carbon footprint, when compared to conventional plastic packaging materials. This review discusses the potential of polysaccharides as a raw material to produce multifunctional materials for food packaging applications. The covered areas include the recent innovations and properties of the polysaccharide-based materials. Emphasis is given to hemicelluloses, marine polysaccharides, and bacterial exopolysaccharides and their potential application in the latest trends of food packaging materials, including edible coatings, intelligent films, and thermo-insulated aerogel packaging.",
journal = "Molecules",
title = "Prospect of Polysaccharide-Based Materials as Advanced Food Packaging",
volume = "25",
number = "1",
pages = "135",
doi = "10.3390/molecules25010135"
}

Nešić, A., Cabrera-Barjas, G., Dimitrijević-Branković, S. I., Davidović, S. Z., Radovanović, N.,& Delattre, C.. (2019). Prospect of Polysaccharide-Based Materials as Advanced Food Packaging. in Molecules, 25(1), 135.
https://doi.org/10.3390/molecules25010135

Nešić A, Cabrera-Barjas G, Dimitrijević-Branković SI, Davidović SZ, Radovanović N, Delattre C. Prospect of Polysaccharide-Based Materials as Advanced Food Packaging. in Molecules. 2019;25(1):135.
doi:10.3390/molecules25010135 .

Nešić, Aleksandra, Cabrera-Barjas, Gustavo, Dimitrijević-Branković, Suzana I., Davidović, Slađana Z., Radovanović, Neda, Delattre, Cédric, "Prospect of Polysaccharide-Based Materials as Advanced Food Packaging" in Molecules, 25, no. 1 (2019):135,
https://doi.org/10.3390/molecules25010135 . .

TY  - JOUR
AU  - Radovanović, Neda
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Gordić, Milan V.
AU  - Petrović, Jelena M.
AU  - Pavlović, Vladimir B.
AU  - Mitrić, Miodrag
AU  - Nešić, Aleksandra
AU  - Dimitrijević-Branković, Suzana I.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8454
AB  - New agar-based composite films with increasing Cu-carbonate and Cu-phosphate mineral phase content were prepared by in situ mineralization and solvent casting method. SEM and optical analysis revealed that Cu-carbonate phase had better compatibility with agar matrix than Cu-phosphate phase. Incorporation of both mineral phases improved mechanical and water vapor barrier properties of the obtained mineralized films, in concentration dependent manner. When 5 mM of carbonate precursor was incorporated into agar matrix, mechanical resistance was enchanced for 44% and water vapor barrier property for 40%. The release of Cu (II) was higher in acidic conditions for both mineralized composites and remained in the range of specific release limits for this metal. In addition, both mineralized composite films exhibited distinctive antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Overall, the Cu-carbonate and Cu-phosphate mineralized agar films showed potential to be used for food packaging materials, agriculture or medical purposes. © 2019 Elsevier Ltd
T2  - European Polymer Journal
T1  - Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase
VL  - 119
SP  - 352
EP  - 358
DO  - 10.1016/j.eurpolymj.2019.08.004
ER  - 

@article{
author = "Radovanović, Neda and Malagurski, Ivana and Lević, Steva and Gordić, Milan V. and Petrović, Jelena M. and Pavlović, Vladimir B. and Mitrić, Miodrag and Nešić, Aleksandra and Dimitrijević-Branković, Suzana I.",
year = "2019",
abstract = "New agar-based composite films with increasing Cu-carbonate and Cu-phosphate mineral phase content were prepared by in situ mineralization and solvent casting method. SEM and optical analysis revealed that Cu-carbonate phase had better compatibility with agar matrix than Cu-phosphate phase. Incorporation of both mineral phases improved mechanical and water vapor barrier properties of the obtained mineralized films, in concentration dependent manner. When 5 mM of carbonate precursor was incorporated into agar matrix, mechanical resistance was enchanced for 44% and water vapor barrier property for 40%. The release of Cu (II) was higher in acidic conditions for both mineralized composites and remained in the range of specific release limits for this metal. In addition, both mineralized composite films exhibited distinctive antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Overall, the Cu-carbonate and Cu-phosphate mineralized agar films showed potential to be used for food packaging materials, agriculture or medical purposes. © 2019 Elsevier Ltd",
journal = "European Polymer Journal",
title = "Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase",
volume = "119",
pages = "352-358",
doi = "10.1016/j.eurpolymj.2019.08.004"
}

Radovanović, N., Malagurski, I., Lević, S., Gordić, M. V., Petrović, J. M., Pavlović, V. B., Mitrić, M., Nešić, A.,& Dimitrijević-Branković, S. I.. (2019). Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase. in European Polymer Journal, 119, 352-358.
https://doi.org/10.1016/j.eurpolymj.2019.08.004

Radovanović N, Malagurski I, Lević S, Gordić MV, Petrović JM, Pavlović VB, Mitrić M, Nešić A, Dimitrijević-Branković SI. Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase. in European Polymer Journal. 2019;119:352-358.
doi:10.1016/j.eurpolymj.2019.08.004 .

Radovanović, Neda, Malagurski, Ivana, Lević, Steva, Gordić, Milan V., Petrović, Jelena M., Pavlović, Vladimir B., Mitrić, Miodrag, Nešić, Aleksandra, Dimitrijević-Branković, Suzana I., "Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase" in European Polymer Journal, 119 (2019):352-358,
https://doi.org/10.1016/j.eurpolymj.2019.08.004 . .

TY  - CONF
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Radovanović, Neda
AU  - Gordić, Milan V.
AU  - Carević, Milica
AU  - Nešić, Aleksandra
AU  - Dimitrijević, Suzana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10723
AB  - Dextransucrase (DS), the extracellular enzyme is of immense industrial importance, due to ability to produce dextran and oligosaccharides (OS). Worldwide interest in OS has been increasing, since they have been accorded the prebiotic status. However, the industrial application of DS for OS synthesis is limited, due to low yield of enzyme production and its low catalytic activity. Hence, there is a great interest for development of new technologies that can provide improved performance of biocatalyst. Enzyme immobilization technology is considered to be a crucial step for cheaper and more efficient usage of DS. Entrapment is one of the widely investigated immobilization methods, where enzymes are enclosed or confined within the polymer matrix without altering their native structure, developing bioreactors for commercial applications. Different matrices such as polyacryl-amide gel, alginate beads and agar–agar have been used for the entrapment of different enzymes and among them agar–agar is a biocompatible, non-toxic and strong solidifying agent for immobilization of various enzymes. In this work, the entrapment of DS was initiated by different quantity (1:9, 1:4 and 1:1) of dialyzed enzyme into agar- agar solution. Agar solution was prepared in distilled water by vigorous shaking at 100°C, autoclaved and was allowed to cool to 40–45°C. Afterwards, enzyme was incorporated and mixed thoroughly. This mixture was immediately poured into a clean Petri plate and left to solidify at room temperature. Polymer films with and without immobilized DS were analyzed in terms of enzyme activity and reusability and mechanical properties (tensile strength, elongation at break and elastic modulus). In order to remove un-entrapped enzyme the films were washed with double deionized water and sodium acetate buffer (pH 5.4) three times prior to enzymatic assay. For quantitative analysis of samples for OS production a Dionex Ultimate 3000 HPLC system was used. Results showed that maximum immobilization yield (74.11%) was achieved by use of 2 % agar and (1:9) enzyme: agar ratio. HPLC analysis confirmed the similar trend of OS formation in immobilized samples compared to free enzyme. The lowest tested fraction of enzyme immobilized into polymer matrix (1:9) improved tensile strength of films in comparison with control film, whereas higher concentration of enzyme led to decrease in mechanical resistance of films. Scanning electron microscopy of agar films before and after DS entrapment revealed significant morphological change on the matrix surface. Considering the economic feasibility, the entrapped DS indicated imperative recycling efficiency up to six reaction cycles. The results of this study revealed that an easily available and inexpensive matrix could be successfully employed for DS immobilization and OS production.
C3  - PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia
T1  - Dextransucrase entrapment as an efficient alternative for increased recycling efficiency of free enzyme within agar-agar film matrix
SP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10723
ER  - 

@conference{
author = "Miljković, Miona and Davidović, Slađana and Radovanović, Neda and Gordić, Milan V. and Carević, Milica and Nešić, Aleksandra and Dimitrijević, Suzana",
year = "2018",
abstract = "Dextransucrase (DS), the extracellular enzyme is of immense industrial importance, due to ability to produce dextran and oligosaccharides (OS). Worldwide interest in OS has been increasing, since they have been accorded the prebiotic status. However, the industrial application of DS for OS synthesis is limited, due to low yield of enzyme production and its low catalytic activity. Hence, there is a great interest for development of new technologies that can provide improved performance of biocatalyst. Enzyme immobilization technology is considered to be a crucial step for cheaper and more efficient usage of DS. Entrapment is one of the widely investigated immobilization methods, where enzymes are enclosed or confined within the polymer matrix without altering their native structure, developing bioreactors for commercial applications. Different matrices such as polyacryl-amide gel, alginate beads and agar–agar have been used for the entrapment of different enzymes and among them agar–agar is a biocompatible, non-toxic and strong solidifying agent for immobilization of various enzymes. In this work, the entrapment of DS was initiated by different quantity (1:9, 1:4 and 1:1) of dialyzed enzyme into agar- agar solution. Agar solution was prepared in distilled water by vigorous shaking at 100°C, autoclaved and was allowed to cool to 40–45°C. Afterwards, enzyme was incorporated and mixed thoroughly. This mixture was immediately poured into a clean Petri plate and left to solidify at room temperature. Polymer films with and without immobilized DS were analyzed in terms of enzyme activity and reusability and mechanical properties (tensile strength, elongation at break and elastic modulus). In order to remove un-entrapped enzyme the films were washed with double deionized water and sodium acetate buffer (pH 5.4) three times prior to enzymatic assay. For quantitative analysis of samples for OS production a Dionex Ultimate 3000 HPLC system was used. Results showed that maximum immobilization yield (74.11%) was achieved by use of 2 % agar and (1:9) enzyme: agar ratio. HPLC analysis confirmed the similar trend of OS formation in immobilized samples compared to free enzyme. The lowest tested fraction of enzyme immobilized into polymer matrix (1:9) improved tensile strength of films in comparison with control film, whereas higher concentration of enzyme led to decrease in mechanical resistance of films. Scanning electron microscopy of agar films before and after DS entrapment revealed significant morphological change on the matrix surface. Considering the economic feasibility, the entrapped DS indicated imperative recycling efficiency up to six reaction cycles. The results of this study revealed that an easily available and inexpensive matrix could be successfully employed for DS immobilization and OS production.",
journal = "PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia",
title = "Dextransucrase entrapment as an efficient alternative for increased recycling efficiency of free enzyme within agar-agar film matrix",
pages = "88",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10723"
}

Miljković, M., Davidović, S., Radovanović, N., Gordić, M. V., Carević, M., Nešić, A.,& Dimitrijević, S.. (2018). Dextransucrase entrapment as an efficient alternative for increased recycling efficiency of free enzyme within agar-agar film matrix. in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia, 88.
https://hdl.handle.net/21.15107/rcub_vinar_10723

Miljković M, Davidović S, Radovanović N, Gordić MV, Carević M, Nešić A, Dimitrijević S. Dextransucrase entrapment as an efficient alternative for increased recycling efficiency of free enzyme within agar-agar film matrix. in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia. 2018;:88.
https://hdl.handle.net/21.15107/rcub_vinar_10723 .

Miljković, Miona, Davidović, Slađana, Radovanović, Neda, Gordić, Milan V., Carević, Milica, Nešić, Aleksandra, Dimitrijević, Suzana, "Dextransucrase entrapment as an efficient alternative for increased recycling efficiency of free enzyme within agar-agar film matrix" in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia (2018):88,
https://hdl.handle.net/21.15107/rcub_vinar_10723 .

TY  - JOUR
AU  - Radovanović, Neda
AU  - Milutinović, Milica
AU  - Mihajlovski, Katarina 
AU  - Jović, Jelena M.
AU  - Nastasijević, Branislav J.
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana I.
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0882401017312664
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7679
AB  - In the current study, the biocontrol potential of a novel strain Bacillus sp. PPM3 isolated from marine sediment from the Red Sea in Hurghada, Egypt is recognized. This novel strain was selected out of 32 isolates based on its ability to suppress the growth of four plant pathogenic fungi: Aspergillus flavus, Fusarium graminearum, Mucor sp. and Alternaria sp. The new marine strain was identified and characterized by phenotypic and molecular approaches. The culture filtrate of Bacillus sp. PPM3 suppressed the growth and spore germination of all tested fungi in vitro with the highest value of inhibition reported for Mucor sp. (97.5%). The antifungal effect of the culture filtrate from the strain PPM3 was due to production of highly stable secondary metabolites resistant to extreme pH, temperature and enzymatic treatments. A PCR analysis confirmed the expression of genes involved in the synthesis of antifungal lipopeptides: iturin, bacillomycin D, mycosubtilin and surfactin. In a greenhouse experiment strain PPM3 effectively reduced disease incidence of F. graminearum in maize plants and displayed additional plant growth stimulating effect. The results show that novel marine strain PPM3 could have a potential in commercial application as biocontrol agent for treatment of various plant diseases caused by soil-borne and postharvest pathogenic fungi.
T2  - Microbial Pathogenesis
T1  - Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment
VL  - 120
SP  - 71
EP  - 78
DO  - 10.1016/j.micpath.2018.04.056
ER  - 

@article{
author = "Radovanović, Neda and Milutinović, Milica and Mihajlovski, Katarina  and Jović, Jelena M. and Nastasijević, Branislav J. and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana I.",
year = "2018",
abstract = "In the current study, the biocontrol potential of a novel strain Bacillus sp. PPM3 isolated from marine sediment from the Red Sea in Hurghada, Egypt is recognized. This novel strain was selected out of 32 isolates based on its ability to suppress the growth of four plant pathogenic fungi: Aspergillus flavus, Fusarium graminearum, Mucor sp. and Alternaria sp. The new marine strain was identified and characterized by phenotypic and molecular approaches. The culture filtrate of Bacillus sp. PPM3 suppressed the growth and spore germination of all tested fungi in vitro with the highest value of inhibition reported for Mucor sp. (97.5%). The antifungal effect of the culture filtrate from the strain PPM3 was due to production of highly stable secondary metabolites resistant to extreme pH, temperature and enzymatic treatments. A PCR analysis confirmed the expression of genes involved in the synthesis of antifungal lipopeptides: iturin, bacillomycin D, mycosubtilin and surfactin. In a greenhouse experiment strain PPM3 effectively reduced disease incidence of F. graminearum in maize plants and displayed additional plant growth stimulating effect. The results show that novel marine strain PPM3 could have a potential in commercial application as biocontrol agent for treatment of various plant diseases caused by soil-borne and postharvest pathogenic fungi.",
journal = "Microbial Pathogenesis",
title = "Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment",
volume = "120",
pages = "71-78",
doi = "10.1016/j.micpath.2018.04.056"
}

Radovanović, N., Milutinović, M., Mihajlovski, K., Jović, J. M., Nastasijević, B. J., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S. I.. (2018). Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment. in Microbial Pathogenesis, 120, 71-78.
https://doi.org/10.1016/j.micpath.2018.04.056

Radovanović N, Milutinović M, Mihajlovski K, Jović JM, Nastasijević BJ, Rajilić-Stojanović M, Dimitrijević-Branković SI. Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment. in Microbial Pathogenesis. 2018;120:71-78.
doi:10.1016/j.micpath.2018.04.056 .

Radovanović, Neda, Milutinović, Milica, Mihajlovski, Katarina , Jović, Jelena M., Nastasijević, Branislav J., Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana I., "Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment" in Microbial Pathogenesis, 120 (2018):71-78,
https://doi.org/10.1016/j.micpath.2018.04.056 . .

TY  - CONF
AU  - Radovanović, Neda
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Malagurski, Ivana
AU  - Gordić, Milan V.
AU  - Nešić, Aleksandra
AU  - Dimitrijević-Branković, Suzana I.
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10724
AB  - Polysaccharide-based materials represent an attractive alternative to plastics, due to their biodegradability, compatibility and great film forming properties1. As they are usually characterized by poor mechanical and barrier properties and lack of functionality, different components must be incorporated into these biopolymer materials in order to improve their properties. In this study, new mineralized, agar-based composite films with increasing Cu-phosphate mineral phase loadings (1, 2.5 and 5 mM) were prepared by in situ mineralization and solvent casting method. The presence of mineral significantly influenced the morphology, properties and fun- ctionality of the obtained composite films. Reinforcement with the Cu-phosphate phase improved in a concentration-dependent manner, optical, mechanical and water vapor barrier properties of the obtained mine- ralized films. In addition Cu-phosphate mineralized agar films exhibited antimicrobial activity against both, Gram positive and Gram negative bacteria, Staphylococcus aureus and Escherichia coli, respectively. The res- ults of this study suggest that agar films mineralized with Cu-phosphate could be potentially used as affordable, eco-friendly and functional food packaging materials with tunable properties. Production procedure offers possibilities for increasing Cu-mineral phase content without compromising properties of the composite films.
C3  - PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia
T1  - Active agar mineralized composite films intended for food packaging
SP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10724
ER  - 

@conference{
author = "Radovanović, Neda and Miljković, Miona and Davidović, Slađana and Malagurski, Ivana and Gordić, Milan V. and Nešić, Aleksandra and Dimitrijević-Branković, Suzana I.",
year = "2018",
abstract = "Polysaccharide-based materials represent an attractive alternative to plastics, due to their biodegradability, compatibility and great film forming properties1. As they are usually characterized by poor mechanical and barrier properties and lack of functionality, different components must be incorporated into these biopolymer materials in order to improve their properties. In this study, new mineralized, agar-based composite films with increasing Cu-phosphate mineral phase loadings (1, 2.5 and 5 mM) were prepared by in situ mineralization and solvent casting method. The presence of mineral significantly influenced the morphology, properties and fun- ctionality of the obtained composite films. Reinforcement with the Cu-phosphate phase improved in a concentration-dependent manner, optical, mechanical and water vapor barrier properties of the obtained mine- ralized films. In addition Cu-phosphate mineralized agar films exhibited antimicrobial activity against both, Gram positive and Gram negative bacteria, Staphylococcus aureus and Escherichia coli, respectively. The res- ults of this study suggest that agar films mineralized with Cu-phosphate could be potentially used as affordable, eco-friendly and functional food packaging materials with tunable properties. Production procedure offers possibilities for increasing Cu-mineral phase content without compromising properties of the composite films.",
journal = "PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia",
title = "Active agar mineralized composite films intended for food packaging",
pages = "94",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10724"
}

Radovanović, N., Miljković, M., Davidović, S., Malagurski, I., Gordić, M. V., Nešić, A.,& Dimitrijević-Branković, S. I.. (2018). Active agar mineralized composite films intended for food packaging. in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia, 94.
https://hdl.handle.net/21.15107/rcub_vinar_10724

Radovanović N, Miljković M, Davidović S, Malagurski I, Gordić MV, Nešić A, Dimitrijević-Branković SI. Active agar mineralized composite films intended for food packaging. in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia. 2018;:94.
https://hdl.handle.net/21.15107/rcub_vinar_10724 .

Radovanović, Neda, Miljković, Miona, Davidović, Slađana, Malagurski, Ivana, Gordić, Milan V., Nešić, Aleksandra, Dimitrijević-Branković, Suzana I., "Active agar mineralized composite films intended for food packaging" in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia (2018):94,
https://hdl.handle.net/21.15107/rcub_vinar_10724 .

TY  - CONF
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Radovanović, Neda
AU  - Gordić, Milan V.
AU  - Nešić, Aleksandra
AU  - Dimitrijević, Suzana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10722
AB  - Current trends in food biotechnology are focused on replacing synthetic polymers used for food packages by the natural ones. Biopolymers are made by sustainable processes in a cheap way. Moreover, they are nontoxic and can serve as a good alternative to traditional (petroleum) food packaging, due to great film-formation properties in a form of edible films and coatings for food products. Various polysaccharides, proteins, and lipids have been intensively investigated as edible materials that could improve the food quality, freshness, and provide food safety. However, natural polymers usually lack of antioxidative and/or antimicrobial properties. Therefore, many synthetic and natural additives can be incorporated into edible films. Among them, extracts of medicinal plants are giving more attention due to their safeness and proved health benefits. The aim of this study was to obtain edible films based on dextran, with antioxidative and antimicrobial pro- perties. Dextran obtained in our lab was used for preparing edible film with sorbitol as a plasticizer, while pulverized waste biomass obtained from the processing of medicinal herb yarrow (Achillea millefolium) was used as a source of polyphenols. It has previously been shown 1 that dextran-based films had excellent mecha- nical and water vapor barrier properties. In this work, spray dried ethanol extract of Achillea millefolium dust (AME) was incorporated into dextran films in concentrations 2, 3, and 4% (w/v). The composite films were analyzed regarding antioxidative, antimicrobial, and mechanical properties. All films showed high antioxidative activity (90% reduction of DPPH radical). However, to obtain high antimicrobial activity (higher than 50%), 4% of AME was required. Composite films showed antimicrobial activity against three Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and Enterococcus faecalis and one Gram-negative bacterium (Pseudomonas aeruginosa). Incorporation of AME into dextran film showed positive effect on tensile strength and negative effect on elongation. Composite film with the best mechanical properties (tensile strength 3.5 MPa and elongation at break 37%) was the film containing 4% AME.
C3  - PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia
T1  - Antioxidant and antimicrobial edible films based on dextran containing polyphenols from yarrow extract
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10722
ER  - 

@conference{
author = "Davidović, Slađana and Miljković, Miona and Radovanović, Neda and Gordić, Milan V. and Nešić, Aleksandra and Dimitrijević, Suzana",
year = "2018",
abstract = "Current trends in food biotechnology are focused on replacing synthetic polymers used for food packages by the natural ones. Biopolymers are made by sustainable processes in a cheap way. Moreover, they are nontoxic and can serve as a good alternative to traditional (petroleum) food packaging, due to great film-formation properties in a form of edible films and coatings for food products. Various polysaccharides, proteins, and lipids have been intensively investigated as edible materials that could improve the food quality, freshness, and provide food safety. However, natural polymers usually lack of antioxidative and/or antimicrobial properties. Therefore, many synthetic and natural additives can be incorporated into edible films. Among them, extracts of medicinal plants are giving more attention due to their safeness and proved health benefits. The aim of this study was to obtain edible films based on dextran, with antioxidative and antimicrobial pro- perties. Dextran obtained in our lab was used for preparing edible film with sorbitol as a plasticizer, while pulverized waste biomass obtained from the processing of medicinal herb yarrow (Achillea millefolium) was used as a source of polyphenols. It has previously been shown 1 that dextran-based films had excellent mecha- nical and water vapor barrier properties. In this work, spray dried ethanol extract of Achillea millefolium dust (AME) was incorporated into dextran films in concentrations 2, 3, and 4% (w/v). The composite films were analyzed regarding antioxidative, antimicrobial, and mechanical properties. All films showed high antioxidative activity (90% reduction of DPPH radical). However, to obtain high antimicrobial activity (higher than 50%), 4% of AME was required. Composite films showed antimicrobial activity against three Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and Enterococcus faecalis and one Gram-negative bacterium (Pseudomonas aeruginosa). Incorporation of AME into dextran film showed positive effect on tensile strength and negative effect on elongation. Composite film with the best mechanical properties (tensile strength 3.5 MPa and elongation at break 37%) was the film containing 4% AME.",
journal = "PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia",
title = "Antioxidant and antimicrobial edible films based on dextran containing polyphenols from yarrow extract",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10722"
}

Davidović, S., Miljković, M., Radovanović, N., Gordić, M. V., Nešić, A.,& Dimitrijević, S.. (2018). Antioxidant and antimicrobial edible films based on dextran containing polyphenols from yarrow extract. in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia, 72.
https://hdl.handle.net/21.15107/rcub_vinar_10722

Davidović S, Miljković M, Radovanović N, Gordić MV, Nešić A, Dimitrijević S. Antioxidant and antimicrobial edible films based on dextran containing polyphenols from yarrow extract. in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia. 2018;:72.
https://hdl.handle.net/21.15107/rcub_vinar_10722 .

Davidović, Slađana, Miljković, Miona, Radovanović, Neda, Gordić, Milan V., Nešić, Aleksandra, Dimitrijević, Suzana, "Antioxidant and antimicrobial edible films based on dextran containing polyphenols from yarrow extract" in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia (2018):72,
https://hdl.handle.net/21.15107/rcub_vinar_10722 .