TY  - CONF
AU  - Vlašković, Tijana
AU  - Laban, Bojana
AU  - Milošević, Maja
AU  - Čebela, Maria
AU  - Dodevski, Vladimir
AU  - Rosić, Milena
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13035
AB  - Nanostrukturni Ca0.9Er0.1MnO3 je sintetisan saharoza-nitratnon procedurom (SNP). Tokom ove metode sagorevanja su korišćene sledeće hemikalije: saharoza C12H22O11 koja ima dvostruku ulogu i goriva i kompleksanta, kalcijum-nitrat tetrahidrat, mangan(II)-nitrat hidrat i  erbijum(III)-nitrat pentahidrat. Nitrati metala i saharoza su kombinovani u njihovim odgovarajućim stehiometrijskim odnosima da bi se pripremio složeni oksid perovskitske nanostrukture. Dobijeni prah Ca0.9Er0.1MnO3 je kalcinisan u temperaturnom opsegu od 800-1000°C tokom 15 min. Ispitani su veličina čestica, parametri rešetke, strukturne promene i površina. Za karakterizaciju sintetisanih uzoraka korišćena je diferencijalno termijska analiza (DTA), rendgenska difrakciona analiza (XRD), Furijeova transformaciona infracrvena spektroskopija (FTIR) i skenirajuća elektronska mikroskopija (SEM).
AB  - Nanostructured Ca0.9Er0.1MnO3 was synthesized by the sucrose-nitrate procedure (SNP). During this combustion method, sucrose C12H22O11 which is both fuel and complexant and metal ions in the form of calcium nitrate tetrahydrate, manganese(II) nitrate hydrate, erbium(III) nitrate pentahydrate were used. Metal nitrates and sucrose were combined to prepare this nanostructured in their appropriate stoichiometric ratios. Obtained Ca0,9Er0,1MnO3 powder was calcined in a temperature range of 800-1000 °C for 15min. Particle size, lattice parameters, structural changes, and specific surface area were investigated. DTA, X-ray diffraction (XRD), FTIR, and Field emission scanning electron microscopy (SEM) were used to characterize the synthesized samples at room temperature.
PB  - Belgrade : Serbian Chemical Society
C3  - 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings
T1  - Sinteza i karakterizacija nanostrukturnog Ca0.9 Er0.1 MnO3
T1  - Synthesis and characterization of nanostructured Ca0.9Er0.1MnO3
SP  - 95
EP  - 95
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13035
ER  - 

@conference{
author = "Vlašković, Tijana and Laban, Bojana and Milošević, Maja and Čebela, Maria and Dodevski, Vladimir and Rosić, Milena",
year = "2023",
abstract = "Nanostrukturni Ca0.9Er0.1MnO3 je sintetisan saharoza-nitratnon procedurom (SNP). Tokom ove metode sagorevanja su korišćene sledeće hemikalije: saharoza C12H22O11 koja ima dvostruku ulogu i goriva i kompleksanta, kalcijum-nitrat tetrahidrat, mangan(II)-nitrat hidrat i  erbijum(III)-nitrat pentahidrat. Nitrati metala i saharoza su kombinovani u njihovim odgovarajućim stehiometrijskim odnosima da bi se pripremio složeni oksid perovskitske nanostrukture. Dobijeni prah Ca0.9Er0.1MnO3 je kalcinisan u temperaturnom opsegu od 800-1000°C tokom 15 min. Ispitani su veličina čestica, parametri rešetke, strukturne promene i površina. Za karakterizaciju sintetisanih uzoraka korišćena je diferencijalno termijska analiza (DTA), rendgenska difrakciona analiza (XRD), Furijeova transformaciona infracrvena spektroskopija (FTIR) i skenirajuća elektronska mikroskopija (SEM)., Nanostructured Ca0.9Er0.1MnO3 was synthesized by the sucrose-nitrate procedure (SNP). During this combustion method, sucrose C12H22O11 which is both fuel and complexant and metal ions in the form of calcium nitrate tetrahydrate, manganese(II) nitrate hydrate, erbium(III) nitrate pentahydrate were used. Metal nitrates and sucrose were combined to prepare this nanostructured in their appropriate stoichiometric ratios. Obtained Ca0,9Er0,1MnO3 powder was calcined in a temperature range of 800-1000 °C for 15min. Particle size, lattice parameters, structural changes, and specific surface area were investigated. DTA, X-ray diffraction (XRD), FTIR, and Field emission scanning electron microscopy (SEM) were used to characterize the synthesized samples at room temperature.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings",
title = "Sinteza i karakterizacija nanostrukturnog Ca0.9 Er0.1 MnO3, Synthesis and characterization of nanostructured Ca0.9Er0.1MnO3",
pages = "95-95",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13035"
}

Vlašković, T., Laban, B., Milošević, M., Čebela, M., Dodevski, V.,& Rosić, M.. (2023). Sinteza i karakterizacija nanostrukturnog Ca0.9 Er0.1 MnO3. in 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings
Belgrade : Serbian Chemical Society., 95-95.
https://hdl.handle.net/21.15107/rcub_vinar_13035

Vlašković T, Laban B, Milošević M, Čebela M, Dodevski V, Rosić M. Sinteza i karakterizacija nanostrukturnog Ca0.9 Er0.1 MnO3. in 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings. 2023;:95-95.
https://hdl.handle.net/21.15107/rcub_vinar_13035 .

Vlašković, Tijana, Laban, Bojana, Milošević, Maja, Čebela, Maria, Dodevski, Vladimir, Rosić, Milena, "Sinteza i karakterizacija nanostrukturnog Ca0.9 Er0.1 MnO3" in 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings (2023):95-95,
https://hdl.handle.net/21.15107/rcub_vinar_13035 .

TY  - CONF
AU  - Rosić, Milena
AU  - Milošević, Maja
AU  - Čebela, Maria
AU  - Dodevski, Vladimir
AU  - Lojpur, Vesna
AU  - Vasić, Marija
AU  - Zarubica, Aleksandra
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11467
AB  - The nanostructured powderwas synthesized by the glycine nitrate procedure (GNP) because it proved to be the most effective and simplest method suitable for controlling the composition and morphology of Co0.9Ho0.1MoO4. To prepare nanostructured Co0.9Ho0.1MoO4, metal nitrates, and glycine were mixed in appropriate stoichiometric ratios. For the preparation of the technologically important Co0.9Ho0.1MoO4, the combustion process has proven to be a promising method that achieves control of stoichiometry, homogeneity, and purity. The synthesized samples were examined by DTA, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectra, Spectroscopy, Field emission scanning electron microscopy (FESEM), and nitrogen adsorption method. The acquired nanopowder showed a tendency for agglomeration, inhomogeneous microstructure, and plate-like crystals. The photocatalytic activity of the obtained Co0.9Ho0.1MoO4nanopowders was evaluated by the photocatalytic degradation of crystal violet in an aqueous solution. After photocatalytic testing and all the above-mentioned characterizations, it was shown that these nanostructured materials represent promising solutions in photocatalytic processes toward green chemistry and sustainable development.
PB  - Belgrade : Serbian Ceramic Society
C3  - Advanced Ceramics and Application XI : Program and the Book of Abstracts ; September 18-20, 2023; Belgrade
T1  - Examination and characterization of nanostructured Co0.9Ho0.1MoO4
SP  - 69
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11467
ER  - 

@conference{
author = "Rosić, Milena and Milošević, Maja and Čebela, Maria and Dodevski, Vladimir and Lojpur, Vesna and Vasić, Marija and Zarubica, Aleksandra",
year = "2023",
abstract = "The nanostructured powderwas synthesized by the glycine nitrate procedure (GNP) because it proved to be the most effective and simplest method suitable for controlling the composition and morphology of Co0.9Ho0.1MoO4. To prepare nanostructured Co0.9Ho0.1MoO4, metal nitrates, and glycine were mixed in appropriate stoichiometric ratios. For the preparation of the technologically important Co0.9Ho0.1MoO4, the combustion process has proven to be a promising method that achieves control of stoichiometry, homogeneity, and purity. The synthesized samples were examined by DTA, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectra, Spectroscopy, Field emission scanning electron microscopy (FESEM), and nitrogen adsorption method. The acquired nanopowder showed a tendency for agglomeration, inhomogeneous microstructure, and plate-like crystals. The photocatalytic activity of the obtained Co0.9Ho0.1MoO4nanopowders was evaluated by the photocatalytic degradation of crystal violet in an aqueous solution. After photocatalytic testing and all the above-mentioned characterizations, it was shown that these nanostructured materials represent promising solutions in photocatalytic processes toward green chemistry and sustainable development.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Advanced Ceramics and Application XI : Program and the Book of Abstracts ; September 18-20, 2023; Belgrade",
title = "Examination and characterization of nanostructured Co0.9Ho0.1MoO4",
pages = "69-69",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11467"
}

Rosić, M., Milošević, M., Čebela, M., Dodevski, V., Lojpur, V., Vasić, M.,& Zarubica, A.. (2023). Examination and characterization of nanostructured Co0.9Ho0.1MoO4. in Advanced Ceramics and Application XI : Program and the Book of Abstracts ; September 18-20, 2023; Belgrade
Belgrade : Serbian Ceramic Society., 69-69.
https://hdl.handle.net/21.15107/rcub_vinar_11467

Rosić M, Milošević M, Čebela M, Dodevski V, Lojpur V, Vasić M, Zarubica A. Examination and characterization of nanostructured Co0.9Ho0.1MoO4. in Advanced Ceramics and Application XI : Program and the Book of Abstracts ; September 18-20, 2023; Belgrade. 2023;:69-69.
https://hdl.handle.net/21.15107/rcub_vinar_11467 .

Rosić, Milena, Milošević, Maja, Čebela, Maria, Dodevski, Vladimir, Lojpur, Vesna, Vasić, Marija, Zarubica, Aleksandra, "Examination and characterization of nanostructured Co0.9Ho0.1MoO4" in Advanced Ceramics and Application XI : Program and the Book of Abstracts ; September 18-20, 2023; Belgrade (2023):69-69,
https://hdl.handle.net/21.15107/rcub_vinar_11467 .

TY  - CONF
AU  - Čebela, Maria
AU  - Čakar, Uroš
AU  - Lojpur, Vesna
AU  - Milošević, Maja
AU  - Krstić, Sanja
AU  - Dodevski, Vladimir
AU  - Rosić, Milena
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11468
AB  - We studied the influence of Ag doping on the crystal structure and magnetic properties of CuO nanopowders. For the synthesis of nanoparticles of copper-silver oxides solid solutions with the composition Cu1-xAgxO (x=0.01–0.05), a self- propagating synthesis was applied at room temperature, during which a successful reaction between metal nitrate and sodium hydroxide occurred. Prepared powders were calcinated at 700 °C for 2 h. The diffraction pattern was recorded at room temperature and atmospheric pressure without of any re-heating of the sample. The Rietveld method for fitting refinement procedure was performed which showed the incorporation of Ag3+ ions in the CuO crystal lattice, where they substitute Cu2+ ions. SQUID magnetometer was used for investigation of magnetic behavior of synthesized materials in temperature interval 2–400 K. It is known that copper(II) oxide exhibits ferroelectricity driven by magnetic order at temperature as high as 230 K [1]. Multiferroic phase is present above the first order phase transition at TN1 = 213 K and exists up to the subsequent first order phase transition TN2 = 230 K [1,2]. It was shown that disorder in the form of impurities can stabilize the ferroelectric phase [2] this was our motivation to dope CuO with Ag in order to improve further its multiferroic properties. Compared with CuO, we observed small changes in magnetic properties in Cu1-xAgxO. The size and morphology of the particles were successfully determined by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
PB  - Belgrade : Institute for Multidisciplinary Research
C3  - Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade
T1  - Influence of Ag doping on the morphological and magnetic properties in CuO nanopowders
SP  - 122
EP  - 122
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11468
ER  - 

@conference{
author = "Čebela, Maria and Čakar, Uroš and Lojpur, Vesna and Milošević, Maja and Krstić, Sanja and Dodevski, Vladimir and Rosić, Milena",
year = "2023",
abstract = "We studied the influence of Ag doping on the crystal structure and magnetic properties of CuO nanopowders. For the synthesis of nanoparticles of copper-silver oxides solid solutions with the composition Cu1-xAgxO (x=0.01–0.05), a self- propagating synthesis was applied at room temperature, during which a successful reaction between metal nitrate and sodium hydroxide occurred. Prepared powders were calcinated at 700 °C for 2 h. The diffraction pattern was recorded at room temperature and atmospheric pressure without of any re-heating of the sample. The Rietveld method for fitting refinement procedure was performed which showed the incorporation of Ag3+ ions in the CuO crystal lattice, where they substitute Cu2+ ions. SQUID magnetometer was used for investigation of magnetic behavior of synthesized materials in temperature interval 2–400 K. It is known that copper(II) oxide exhibits ferroelectricity driven by magnetic order at temperature as high as 230 K [1]. Multiferroic phase is present above the first order phase transition at TN1 = 213 K and exists up to the subsequent first order phase transition TN2 = 230 K [1,2]. It was shown that disorder in the form of impurities can stabilize the ferroelectric phase [2] this was our motivation to dope CuO with Ag in order to improve further its multiferroic properties. Compared with CuO, we observed small changes in magnetic properties in Cu1-xAgxO. The size and morphology of the particles were successfully determined by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).",
publisher = "Belgrade : Institute for Multidisciplinary Research",
journal = "Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade",
title = "Influence of Ag doping on the morphological and magnetic properties in CuO nanopowders",
pages = "122-122",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11468"
}

Čebela, M., Čakar, U., Lojpur, V., Milošević, M., Krstić, S., Dodevski, V.,& Rosić, M.. (2023). Influence of Ag doping on the morphological and magnetic properties in CuO nanopowders. in Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade
Belgrade : Institute for Multidisciplinary Research., 122-122.
https://hdl.handle.net/21.15107/rcub_vinar_11468

Čebela M, Čakar U, Lojpur V, Milošević M, Krstić S, Dodevski V, Rosić M. Influence of Ag doping on the morphological and magnetic properties in CuO nanopowders. in Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade. 2023;:122-122.
https://hdl.handle.net/21.15107/rcub_vinar_11468 .

Čebela, Maria, Čakar, Uroš, Lojpur, Vesna, Milošević, Maja, Krstić, Sanja, Dodevski, Vladimir, Rosić, Milena, "Influence of Ag doping on the morphological and magnetic properties in CuO nanopowders" in Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade (2023):122-122,
https://hdl.handle.net/21.15107/rcub_vinar_11468 .

TY  - CONF
AU  - Rosić, Milena
AU  - Milošević, Maja
AU  - Čebela, Maria
AU  - Dodevski, Vladimir
AU  - Lojpur, Vesna
AU  - Ljupković, Radomir
AU  - Zarubica, Aleksandra
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11490
AB  - Co0.9Ho0.1MoO4 nanopowders were obtained by applying glycine nitrate procedure (GNP). The synthesized samples were investigated by DTA, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectra, Spectroscopy, Field emission scanning electron microscopy (FESEM), and nitrogen adsorption method. The photocatalytic activity of acquired Co0.9Ho0.1MoO4 nanopowders was estimated by the photocatalytic degradation of crystal violet in aqueous solution. A simple and effective method for controlling the composition and morphology of Co0.9Ho0.1MoO4 is presented in this paper, as well as a potentially new approach in the methodology of inorganic synthesis. During photocatalytic testing nanostructured material Co0.9Ho0.1MoO4 indicated the possibility of a promising solution in photocatalytic processes towards green chemistry and sustainable development.
PB  - Belgrade : University of Belgrade, Institute for Multidisciplinary Research
C3  - Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade
T1  - Synthesis, characterization and photocatalytic examination of Co0.9Ho0.1MoO4 nanopowders
SP  - 81
EP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11490
ER  - 

@conference{
author = "Rosić, Milena and Milošević, Maja and Čebela, Maria and Dodevski, Vladimir and Lojpur, Vesna and Ljupković, Radomir and Zarubica, Aleksandra",
year = "2023",
abstract = "Co0.9Ho0.1MoO4 nanopowders were obtained by applying glycine nitrate procedure (GNP). The synthesized samples were investigated by DTA, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectra, Spectroscopy, Field emission scanning electron microscopy (FESEM), and nitrogen adsorption method. The photocatalytic activity of acquired Co0.9Ho0.1MoO4 nanopowders was estimated by the photocatalytic degradation of crystal violet in aqueous solution. A simple and effective method for controlling the composition and morphology of Co0.9Ho0.1MoO4 is presented in this paper, as well as a potentially new approach in the methodology of inorganic synthesis. During photocatalytic testing nanostructured material Co0.9Ho0.1MoO4 indicated the possibility of a promising solution in photocatalytic processes towards green chemistry and sustainable development.",
publisher = "Belgrade : University of Belgrade, Institute for Multidisciplinary Research",
journal = "Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade",
title = "Synthesis, characterization and photocatalytic examination of Co0.9Ho0.1MoO4 nanopowders",
pages = "81-81",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11490"
}

Rosić, M., Milošević, M., Čebela, M., Dodevski, V., Lojpur, V., Ljupković, R.,& Zarubica, A.. (2023). Synthesis, characterization and photocatalytic examination of Co0.9Ho0.1MoO4 nanopowders. in Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade
Belgrade : University of Belgrade, Institute for Multidisciplinary Research., 81-81.
https://hdl.handle.net/21.15107/rcub_vinar_11490

Rosić M, Milošević M, Čebela M, Dodevski V, Lojpur V, Ljupković R, Zarubica A. Synthesis, characterization and photocatalytic examination of Co0.9Ho0.1MoO4 nanopowders. in Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade. 2023;:81-81.
https://hdl.handle.net/21.15107/rcub_vinar_11490 .

Rosić, Milena, Milošević, Maja, Čebela, Maria, Dodevski, Vladimir, Lojpur, Vesna, Ljupković, Radomir, Zarubica, Aleksandra, "Synthesis, characterization and photocatalytic examination of Co0.9Ho0.1MoO4 nanopowders" in Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade (2023):81-81,
https://hdl.handle.net/21.15107/rcub_vinar_11490 .

TY  - CONF
AU  - Vlašković, Tijana
AU  - Laban, Bojana
AU  - Milošević, Maja
AU  - Čebela, Maria
AU  - Dodevski, Vladimir
AU  - Rosić, Milena
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11648
AB  - Nano-crystalline Ca0.9Er0.1MnO3 oxide with a perovskite structure was synthesized by the sucrose nitrate procedure (SNP), with the help of sucrose, which plays the role of fuel and complexant. Other chemicals used are calcium nitrate tetrahydrate Ca(NO3)2×4H2O, manganese nitrate hydrate Mn(NO3)2×H2O, erbium nitrate pentahydrate Er(NO3)3×5H2O. Metal nitrates and sucrose were mixed in stoichiometric ratios, in order to obtain a perovskite with a crystalline structure. The resulting Ca0.9Er0.1MnO3 is calcined for 15 minutes in the temperature range from 800°C to 1000°C. Diffraction thermal analysis (DTA), X-ray diffraction analysis (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and inductively coupled plasma ICP were used to characterize the obtained powder.
PB  - Belgrade : Serbian Ceramic Society
C3  - Advanced Ceramics and Application :11th Serbian Ceramic Society Conference : program and the book of abstracts; September 18-20, 2023; Belgrade
T1  - Investigation of nanostructured Ca0.9Er0.1MnO3 obtained by sucrose nitrate procedure
SP  - 74
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11648
ER  - 

@conference{
author = "Vlašković, Tijana and Laban, Bojana and Milošević, Maja and Čebela, Maria and Dodevski, Vladimir and Rosić, Milena",
year = "2023",
abstract = "Nano-crystalline Ca0.9Er0.1MnO3 oxide with a perovskite structure was synthesized by the sucrose nitrate procedure (SNP), with the help of sucrose, which plays the role of fuel and complexant. Other chemicals used are calcium nitrate tetrahydrate Ca(NO3)2×4H2O, manganese nitrate hydrate Mn(NO3)2×H2O, erbium nitrate pentahydrate Er(NO3)3×5H2O. Metal nitrates and sucrose were mixed in stoichiometric ratios, in order to obtain a perovskite with a crystalline structure. The resulting Ca0.9Er0.1MnO3 is calcined for 15 minutes in the temperature range from 800°C to 1000°C. Diffraction thermal analysis (DTA), X-ray diffraction analysis (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and inductively coupled plasma ICP were used to characterize the obtained powder.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Advanced Ceramics and Application :11th Serbian Ceramic Society Conference : program and the book of abstracts; September 18-20, 2023; Belgrade",
title = "Investigation of nanostructured Ca0.9Er0.1MnO3 obtained by sucrose nitrate procedure",
pages = "74",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11648"
}

Vlašković, T., Laban, B., Milošević, M., Čebela, M., Dodevski, V.,& Rosić, M.. (2023). Investigation of nanostructured Ca0.9Er0.1MnO3 obtained by sucrose nitrate procedure. in Advanced Ceramics and Application :11th Serbian Ceramic Society Conference : program and the book of abstracts; September 18-20, 2023; Belgrade
Belgrade : Serbian Ceramic Society., 74.
https://hdl.handle.net/21.15107/rcub_vinar_11648

Vlašković T, Laban B, Milošević M, Čebela M, Dodevski V, Rosić M. Investigation of nanostructured Ca0.9Er0.1MnO3 obtained by sucrose nitrate procedure. in Advanced Ceramics and Application :11th Serbian Ceramic Society Conference : program and the book of abstracts; September 18-20, 2023; Belgrade. 2023;:74.
https://hdl.handle.net/21.15107/rcub_vinar_11648 .

Vlašković, Tijana, Laban, Bojana, Milošević, Maja, Čebela, Maria, Dodevski, Vladimir, Rosić, Milena, "Investigation of nanostructured Ca0.9Er0.1MnO3 obtained by sucrose nitrate procedure" in Advanced Ceramics and Application :11th Serbian Ceramic Society Conference : program and the book of abstracts; September 18-20, 2023; Belgrade (2023):74,
https://hdl.handle.net/21.15107/rcub_vinar_11648 .

TY  - CONF
AU  - Vlašković, Tijana B.
AU  - Laban, Bojana
AU  - Milošević, Maja
AU  - Čebela, Maria
AU  - Dodevski, Vladimir
AU  - Rosić, Milena
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11743
AB  - The present research demonstrates the synthesis and characterization of Ca0.9Er0.1MnO3 perovskite powder using the sucrose nitrate procedure (SNP) technique. The following substances were used to obtain this complex perovskite: sucrose C12H22O11, which has a dual role (complexant and fuel), and metal nitrates were used as oxidants - calcium nitrate tetrahydrate Ca(NO3)2×4H2O, manganese(II) nitrate hydrate Mn(NO3)2×H2O, erbium(III) nitrate pentahydrate Er(NO3)3×5H2O. Nanopowder was prepared by combining metal nitrates in their respective stoichiometric ratios. The synthesized Ca0.9Er0.1MnO3 powder was calcined in a temperature range of 800–1000 °C for a period of 15 min. The effects of calcination were characterized through different experimental techniques (differential thermal analysis (DTA), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (FESEM), and inductively coupled plasma (ICP)).
PB  - Novi Sad : Faculty of Technology, University of Novi Sad
C3  - CYSC-2023 : 15th EcerS Conference for Young Scientists in Ceramics, programme and book of abstracts; Oct 11-14, Novi Sad
T1  - Preparation, synthesis and characterization of nanometric Ca0.9Er0.1MnO3
SP  - 65
EP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11743
ER  - 

@conference{
author = "Vlašković, Tijana B. and Laban, Bojana and Milošević, Maja and Čebela, Maria and Dodevski, Vladimir and Rosić, Milena",
year = "2023",
abstract = "The present research demonstrates the synthesis and characterization of Ca0.9Er0.1MnO3 perovskite powder using the sucrose nitrate procedure (SNP) technique. The following substances were used to obtain this complex perovskite: sucrose C12H22O11, which has a dual role (complexant and fuel), and metal nitrates were used as oxidants - calcium nitrate tetrahydrate Ca(NO3)2×4H2O, manganese(II) nitrate hydrate Mn(NO3)2×H2O, erbium(III) nitrate pentahydrate Er(NO3)3×5H2O. Nanopowder was prepared by combining metal nitrates in their respective stoichiometric ratios. The synthesized Ca0.9Er0.1MnO3 powder was calcined in a temperature range of 800–1000 °C for a period of 15 min. The effects of calcination were characterized through different experimental techniques (differential thermal analysis (DTA), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (FESEM), and inductively coupled plasma (ICP)).",
publisher = "Novi Sad : Faculty of Technology, University of Novi Sad",
journal = "CYSC-2023 : 15th EcerS Conference for Young Scientists in Ceramics, programme and book of abstracts; Oct 11-14, Novi Sad",
title = "Preparation, synthesis and characterization of nanometric Ca0.9Er0.1MnO3",
pages = "65-65",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11743"
}

Vlašković, T. B., Laban, B., Milošević, M., Čebela, M., Dodevski, V.,& Rosić, M.. (2023). Preparation, synthesis and characterization of nanometric Ca0.9Er0.1MnO3. in CYSC-2023 : 15th EcerS Conference for Young Scientists in Ceramics, programme and book of abstracts; Oct 11-14, Novi Sad
Novi Sad : Faculty of Technology, University of Novi Sad., 65-65.
https://hdl.handle.net/21.15107/rcub_vinar_11743

Vlašković TB, Laban B, Milošević M, Čebela M, Dodevski V, Rosić M. Preparation, synthesis and characterization of nanometric Ca0.9Er0.1MnO3. in CYSC-2023 : 15th EcerS Conference for Young Scientists in Ceramics, programme and book of abstracts; Oct 11-14, Novi Sad. 2023;:65-65.
https://hdl.handle.net/21.15107/rcub_vinar_11743 .

Vlašković, Tijana B., Laban, Bojana, Milošević, Maja, Čebela, Maria, Dodevski, Vladimir, Rosić, Milena, "Preparation, synthesis and characterization of nanometric Ca0.9Er0.1MnO3" in CYSC-2023 : 15th EcerS Conference for Young Scientists in Ceramics, programme and book of abstracts; Oct 11-14, Novi Sad (2023):65-65,
https://hdl.handle.net/21.15107/rcub_vinar_11743 .

TY  - JOUR
AU  - Nišavić, Marija
AU  - Stoiljković, Milovan
AU  - Crnolatac, Ivo
AU  - Milošević, Maja
AU  - Rilak, Ana
AU  - Masnikosa, Romana
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1878535216301198
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7665
AB  - Three coordination compounds of ruthenium(II), belonging to a recently synthesised series of water-soluble compounds of general formula mer-{[}Ru(L3)(N-N) Cl] Cl, where L3 = 4'-chloro2,2': 6', 200-terpyridine (Cl-tpy), N-N= ethylenediamine (en), 1,2-diaminocyclohexane (dach) or 2,2'bipyridine (bpy), have shown strong binding to calf thymus DNA and moderate in vitro cytotoxicity towards cancer cell lines. Knowing that serum proteins play a crucial role in the transport and deactivation of ruthenium drugs, we have conducted a detailed study of their interactions with two major metal-transporting serum proteins, albumin and transferrin, and it is presented herein. Ruthenated protein adducts were formed with various concentrations of the three compounds and then separated from the unbound portions by ultrafiltration through 10 kDa cut-off centrifugal filter units. The stoichiometry of binding was determined using inductively coupled plasma optical emission spectrometry. One mol of albumin bound up to 7, 8.5 and 1.5 mol of compound 1 ({[}Ru(Cltpy)(en) Cl]{[}Cl]), 2 ({[}Ru(Cl-tpy)(dach) Cl]{[}Cl] and 3 ({[}Ru(Cl-tpy)(bpy) Cl]{[}Cl]), respectively. One mol of transferrin bound up to 3, 3.5 and 0.4 mol of 1, 2 and 3, respectively. The affinity of albumin and transferrin for the three ruthenium compounds was evaluated using fluorescence quenching. The binding constants for 1 and 2 lay within the range 10(4) -10(5) M - 1, suggesting moderate-to-strong attachment to albumin. Both compounds showed much lower affinity for transferrin (10(2) -10(3) M - 1). Compound 3 bound weakly to each studied protein. High resolution ESI qTOF mass spectra of albumin before and after binding of 1 revealed the high stoichiometry of binding. Although the binding of the compounds 1-3 to albumin and transferrin did not affect proteins' secondary structure much, their tertiary structures underwent some alterations, as deduced from the circular dichroism study. Changes in the stability of albumin, after binding to compounds 1-3 were examined by differential scanning calorimetry. (C) 2016 The Authors. Production and hosting by Elsevier B. V. on behalf of King Saud University. This is an open access article under theCCBY-NC-NDlicense.
T2  - Arabian Journal of Chemistry
T1  - Highly water-soluble ruthenium(II) terpyridine coordination compounds form stable adducts with blood-borne metal transporting proteins
VL  - 11
IS  - 3
SP  - 291
EP  - 304
DO  - 10.1016/j.arabjc.2016.07.021
ER  - 

@article{
author = "Nišavić, Marija and Stoiljković, Milovan and Crnolatac, Ivo and Milošević, Maja and Rilak, Ana and Masnikosa, Romana",
year = "2018",
abstract = "Three coordination compounds of ruthenium(II), belonging to a recently synthesised series of water-soluble compounds of general formula mer-{[}Ru(L3)(N-N) Cl] Cl, where L3 = 4'-chloro2,2': 6', 200-terpyridine (Cl-tpy), N-N= ethylenediamine (en), 1,2-diaminocyclohexane (dach) or 2,2'bipyridine (bpy), have shown strong binding to calf thymus DNA and moderate in vitro cytotoxicity towards cancer cell lines. Knowing that serum proteins play a crucial role in the transport and deactivation of ruthenium drugs, we have conducted a detailed study of their interactions with two major metal-transporting serum proteins, albumin and transferrin, and it is presented herein. Ruthenated protein adducts were formed with various concentrations of the three compounds and then separated from the unbound portions by ultrafiltration through 10 kDa cut-off centrifugal filter units. The stoichiometry of binding was determined using inductively coupled plasma optical emission spectrometry. One mol of albumin bound up to 7, 8.5 and 1.5 mol of compound 1 ({[}Ru(Cltpy)(en) Cl]{[}Cl]), 2 ({[}Ru(Cl-tpy)(dach) Cl]{[}Cl] and 3 ({[}Ru(Cl-tpy)(bpy) Cl]{[}Cl]), respectively. One mol of transferrin bound up to 3, 3.5 and 0.4 mol of 1, 2 and 3, respectively. The affinity of albumin and transferrin for the three ruthenium compounds was evaluated using fluorescence quenching. The binding constants for 1 and 2 lay within the range 10(4) -10(5) M - 1, suggesting moderate-to-strong attachment to albumin. Both compounds showed much lower affinity for transferrin (10(2) -10(3) M - 1). Compound 3 bound weakly to each studied protein. High resolution ESI qTOF mass spectra of albumin before and after binding of 1 revealed the high stoichiometry of binding. Although the binding of the compounds 1-3 to albumin and transferrin did not affect proteins' secondary structure much, their tertiary structures underwent some alterations, as deduced from the circular dichroism study. Changes in the stability of albumin, after binding to compounds 1-3 were examined by differential scanning calorimetry. (C) 2016 The Authors. Production and hosting by Elsevier B. V. on behalf of King Saud University. This is an open access article under theCCBY-NC-NDlicense.",
journal = "Arabian Journal of Chemistry",
title = "Highly water-soluble ruthenium(II) terpyridine coordination compounds form stable adducts with blood-borne metal transporting proteins",
volume = "11",
number = "3",
pages = "291-304",
doi = "10.1016/j.arabjc.2016.07.021"
}

Nišavić, M., Stoiljković, M., Crnolatac, I., Milošević, M., Rilak, A.,& Masnikosa, R.. (2018). Highly water-soluble ruthenium(II) terpyridine coordination compounds form stable adducts with blood-borne metal transporting proteins. in Arabian Journal of Chemistry, 11(3), 291-304.
https://doi.org/10.1016/j.arabjc.2016.07.021

Nišavić M, Stoiljković M, Crnolatac I, Milošević M, Rilak A, Masnikosa R. Highly water-soluble ruthenium(II) terpyridine coordination compounds form stable adducts with blood-borne metal transporting proteins. in Arabian Journal of Chemistry. 2018;11(3):291-304.
doi:10.1016/j.arabjc.2016.07.021 .

Nišavić, Marija, Stoiljković, Milovan, Crnolatac, Ivo, Milošević, Maja, Rilak, Ana, Masnikosa, Romana, "Highly water-soluble ruthenium(II) terpyridine coordination compounds form stable adducts with blood-borne metal transporting proteins" in Arabian Journal of Chemistry, 11, no. 3 (2018):291-304,
https://doi.org/10.1016/j.arabjc.2016.07.021 . .

TY  - JOUR
AU  - Šaponjić, Aleksandra
AU  - Šaponjić, Đorđe
AU  - Nikolić, Violeta N.
AU  - Milošević, Maja
AU  - Marinović-Cincović, Milena
AU  - Gyoshev, Stanislav
AU  - Vuković, Marina
AU  - Kokunešoski, Maja
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1644
AB  - Amorphous iron (III) oxide was obtained from clay, using ammonium hydroxide as a precipitating agent. Influence of freeze drying under vacuum, as a drying method, on particle size, chemical composition, and crystallinity of obtained iron (III) oxide powder was investigated. After freeze drying, precipitate was annealed in air at 500 C-o and 900 oC. X-ray diffraction, particle size analysis, scanning electron microscopy, energy dispersive spectrometry, Fourier transform infrared spectroscopy, thermogravimetric and differential thermal analysis were used to characterize obtained iron (III) oxide powder. All of three powders obtained by freeze drying and annealing, have low crystallinity and particles with irregular layered shape. Narrow particle size distribution was given by an average diameter value of around 50 mu m for all observed powders. Iron-bearing materials like a-Fe2O3 and gamma-Fe2O3 are obtained. Differential thermal analysis curve of obtained samples showed endothermic reaction at 620 oC which could be ascribed to phase transition from cubic form gamma- - GT alpha- Fe2O3. Thermal transformations of iron (III) oxide, obtained from clay as a natural source, is suitable to explore in the framework of materials chemistry, and opens the possibility to synthesize materials based on Fe2O3 with specific magnetic behavior.
T2  - Science of Sintering
T1  - Iron (III) Oxide Fabrication From Natural Clay With Reference to Phase Transformation gamma- - GT alpha- Fe2O3
VL  - 49
IS  - 2
SP  - 197
EP  - 205
DO  - 10.2298/SOS1702197S
ER  - 

@article{
author = "Šaponjić, Aleksandra and Šaponjić, Đorđe and Nikolić, Violeta N. and Milošević, Maja and Marinović-Cincović, Milena and Gyoshev, Stanislav and Vuković, Marina and Kokunešoski, Maja",
year = "2017",
abstract = "Amorphous iron (III) oxide was obtained from clay, using ammonium hydroxide as a precipitating agent. Influence of freeze drying under vacuum, as a drying method, on particle size, chemical composition, and crystallinity of obtained iron (III) oxide powder was investigated. After freeze drying, precipitate was annealed in air at 500 C-o and 900 oC. X-ray diffraction, particle size analysis, scanning electron microscopy, energy dispersive spectrometry, Fourier transform infrared spectroscopy, thermogravimetric and differential thermal analysis were used to characterize obtained iron (III) oxide powder. All of three powders obtained by freeze drying and annealing, have low crystallinity and particles with irregular layered shape. Narrow particle size distribution was given by an average diameter value of around 50 mu m for all observed powders. Iron-bearing materials like a-Fe2O3 and gamma-Fe2O3 are obtained. Differential thermal analysis curve of obtained samples showed endothermic reaction at 620 oC which could be ascribed to phase transition from cubic form gamma- - GT alpha- Fe2O3. Thermal transformations of iron (III) oxide, obtained from clay as a natural source, is suitable to explore in the framework of materials chemistry, and opens the possibility to synthesize materials based on Fe2O3 with specific magnetic behavior.",
journal = "Science of Sintering",
title = "Iron (III) Oxide Fabrication From Natural Clay With Reference to Phase Transformation gamma- - GT alpha- Fe2O3",
volume = "49",
number = "2",
pages = "197-205",
doi = "10.2298/SOS1702197S"
}

Šaponjić, A., Šaponjić, Đ., Nikolić, V. N., Milošević, M., Marinović-Cincović, M., Gyoshev, S., Vuković, M.,& Kokunešoski, M.. (2017). Iron (III) Oxide Fabrication From Natural Clay With Reference to Phase Transformation gamma- - GT alpha- Fe2O3. in Science of Sintering, 49(2), 197-205.
https://doi.org/10.2298/SOS1702197S

Šaponjić A, Šaponjić Đ, Nikolić VN, Milošević M, Marinović-Cincović M, Gyoshev S, Vuković M, Kokunešoski M. Iron (III) Oxide Fabrication From Natural Clay With Reference to Phase Transformation gamma- - GT alpha- Fe2O3. in Science of Sintering. 2017;49(2):197-205.
doi:10.2298/SOS1702197S .

Šaponjić, Aleksandra, Šaponjić, Đorđe, Nikolić, Violeta N., Milošević, Maja, Marinović-Cincović, Milena, Gyoshev, Stanislav, Vuković, Marina, Kokunešoski, Maja, "Iron (III) Oxide Fabrication From Natural Clay With Reference to Phase Transformation gamma- - GT alpha- Fe2O3" in Science of Sintering, 49, no. 2 (2017):197-205,
https://doi.org/10.2298/SOS1702197S . .

TY  - JOUR
AU  - Cvetković, Zorica
AU  - Milošević, Maja
AU  - Cvetković, Bora
AU  - Masnikosa, Romana
AU  - Arsić, Aleksandra
AU  - Petrović, Snježana
AU  - Vučić, Vesna
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1489
AB  - Limited studies have been performed to associate abnormal phospholipid (PL) profile and disease activity in hematological malignancies, including non-Hodgkin lymphoma (NHL). The aim of his study was to evaluate the levels of plasma PL fractions in NHL patients, in response to chemotherapy. Forty non-treated patients with NHL and 25 healthy individuals were recruited. Blood samples from patients were taken before chemotherapy, after 3 cycles and after the end of the treatment, and PL fractions were resolved by one-dimensional thin-layer chromatography. To assess potential relationship between plasma PL profile and response to therapy, patients were divided according to clinical outcome in 3 groups: complete remission (CR), stable disease (SD) and progression (PG). Despite significant differences between NHL patients and healthy controls, no differences were found at baseline among patients divided according to clinical outcome. During and after chemotherapy important alterations in PL profile were observed. Levels of total PLs and all PL fractions decreased in patients with PG while in patients who responded to therapy (CR, SD) PLs significantly increased. Results of our study suggest that changes of total PLs and PL fractions during the therapy are associated with the effects of therapy and clinical outcome in patients with NHL. (C) 2017 Elsevier Ltd. All rights reserved.
T2  - Leukemia Research
T1  - Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients
VL  - 54
SP  - 39
EP  - 46
DO  - 10.1016/j.leukres.2017.01.004
ER  - 

@article{
author = "Cvetković, Zorica and Milošević, Maja and Cvetković, Bora and Masnikosa, Romana and Arsić, Aleksandra and Petrović, Snježana and Vučić, Vesna",
year = "2017",
abstract = "Limited studies have been performed to associate abnormal phospholipid (PL) profile and disease activity in hematological malignancies, including non-Hodgkin lymphoma (NHL). The aim of his study was to evaluate the levels of plasma PL fractions in NHL patients, in response to chemotherapy. Forty non-treated patients with NHL and 25 healthy individuals were recruited. Blood samples from patients were taken before chemotherapy, after 3 cycles and after the end of the treatment, and PL fractions were resolved by one-dimensional thin-layer chromatography. To assess potential relationship between plasma PL profile and response to therapy, patients were divided according to clinical outcome in 3 groups: complete remission (CR), stable disease (SD) and progression (PG). Despite significant differences between NHL patients and healthy controls, no differences were found at baseline among patients divided according to clinical outcome. During and after chemotherapy important alterations in PL profile were observed. Levels of total PLs and all PL fractions decreased in patients with PG while in patients who responded to therapy (CR, SD) PLs significantly increased. Results of our study suggest that changes of total PLs and PL fractions during the therapy are associated with the effects of therapy and clinical outcome in patients with NHL. (C) 2017 Elsevier Ltd. All rights reserved.",
journal = "Leukemia Research",
title = "Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients",
volume = "54",
pages = "39-46",
doi = "10.1016/j.leukres.2017.01.004"
}

Cvetković, Z., Milošević, M., Cvetković, B., Masnikosa, R., Arsić, A., Petrović, S.,& Vučić, V.. (2017). Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients. in Leukemia Research, 54, 39-46.
https://doi.org/10.1016/j.leukres.2017.01.004

Cvetković Z, Milošević M, Cvetković B, Masnikosa R, Arsić A, Petrović S, Vučić V. Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients. in Leukemia Research. 2017;54:39-46.
doi:10.1016/j.leukres.2017.01.004 .

Cvetković, Zorica, Milošević, Maja, Cvetković, Bora, Masnikosa, Romana, Arsić, Aleksandra, Petrović, Snježana, Vučić, Vesna, "Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients" in Leukemia Research, 54 (2017):39-46,
https://doi.org/10.1016/j.leukres.2017.01.004 . .

TY  - JOUR
AU  - Nastasijević, Branislav J.
AU  - Milošević, Maja
AU  - Janjić, Goran V.
AU  - Stanić, Vojislav
AU  - Vasić, Vesna M.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1197
AB  - The extracellular nucleotides act as neurotransmitters and signaling molecules in CNS, binding to a P2X and P2Y receptors. Their concentration regulates a cascade of membrane ecto enzymes, including the ecto-nucleotide triphosphate diphosphohydrolases (E-NTPDases). In many neuropathological conditions, such as neuroinflammatory, epilepsy, depression and migraine, altering of E-NTPDase activity was observed. The objective of this study was to investigate whether Gentiana lutea (G. lutea) extracts affect E-NTPDase activity and which of their constituents (loganic acid, gentiopicroside, isovitexin, amarogentin and isogentisin) exert inhibitory activity. The constituents concentration in the extracts was determined by ultra performance liquid chromatography coupled with mass spectrometry (UPLC-MS). Extracts and constituents were tested with E-NTPDase displayed on the rat synaptosomal membrane as well as by molecular docking study. Ethanol water extract (50%, v/v) exerted significant level of inhibition (52%) at concentration of 200 mg mL(-1). By inhibition studies with single constituents about 30% inhibition was achieved in any case, thus the model of one substrate acting on two enzymes was used to determine IC50 values. Molecular docking study revealed amarogentin, isovitexin and isogentisin dimer as the potent E-NTPDase inhibitors with the binding energies ranging from -9.4 to -10 kcal mol(-1) versus -8.0 kcal mol(-1) for ATP. Presence of isogentisin only in ethanol water extracts may explain their better inhibitory acitivities. Findings of this study are useful from the perspective of safety of products based on G. lutea extracts, while investigated constituents belong to secoiridoids and xanthones class of compounds could be considered as a source of potential E-NTPDase inhibitors.
T2  - International Journal of Pharmacology
T1  - Gentiana lutea Extracts and their Constituents as Inhibitors of Synaptosomal Ecto-NTPDase
VL  - 12
IS  - 4
SP  - 272
EP  - 289
DO  - 10.3923/ijp.2016.272.289
ER  - 

@article{
author = "Nastasijević, Branislav J. and Milošević, Maja and Janjić, Goran V. and Stanić, Vojislav and Vasić, Vesna M.",
year = "2016",
abstract = "The extracellular nucleotides act as neurotransmitters and signaling molecules in CNS, binding to a P2X and P2Y receptors. Their concentration regulates a cascade of membrane ecto enzymes, including the ecto-nucleotide triphosphate diphosphohydrolases (E-NTPDases). In many neuropathological conditions, such as neuroinflammatory, epilepsy, depression and migraine, altering of E-NTPDase activity was observed. The objective of this study was to investigate whether Gentiana lutea (G. lutea) extracts affect E-NTPDase activity and which of their constituents (loganic acid, gentiopicroside, isovitexin, amarogentin and isogentisin) exert inhibitory activity. The constituents concentration in the extracts was determined by ultra performance liquid chromatography coupled with mass spectrometry (UPLC-MS). Extracts and constituents were tested with E-NTPDase displayed on the rat synaptosomal membrane as well as by molecular docking study. Ethanol water extract (50%, v/v) exerted significant level of inhibition (52%) at concentration of 200 mg mL(-1). By inhibition studies with single constituents about 30% inhibition was achieved in any case, thus the model of one substrate acting on two enzymes was used to determine IC50 values. Molecular docking study revealed amarogentin, isovitexin and isogentisin dimer as the potent E-NTPDase inhibitors with the binding energies ranging from -9.4 to -10 kcal mol(-1) versus -8.0 kcal mol(-1) for ATP. Presence of isogentisin only in ethanol water extracts may explain their better inhibitory acitivities. Findings of this study are useful from the perspective of safety of products based on G. lutea extracts, while investigated constituents belong to secoiridoids and xanthones class of compounds could be considered as a source of potential E-NTPDase inhibitors.",
journal = "International Journal of Pharmacology",
title = "Gentiana lutea Extracts and their Constituents as Inhibitors of Synaptosomal Ecto-NTPDase",
volume = "12",
number = "4",
pages = "272-289",
doi = "10.3923/ijp.2016.272.289"
}

Nastasijević, B. J., Milošević, M., Janjić, G. V., Stanić, V.,& Vasić, V. M.. (2016). Gentiana lutea Extracts and their Constituents as Inhibitors of Synaptosomal Ecto-NTPDase. in International Journal of Pharmacology, 12(4), 272-289.
https://doi.org/10.3923/ijp.2016.272.289

Nastasijević BJ, Milošević M, Janjić GV, Stanić V, Vasić VM. Gentiana lutea Extracts and their Constituents as Inhibitors of Synaptosomal Ecto-NTPDase. in International Journal of Pharmacology. 2016;12(4):272-289.
doi:10.3923/ijp.2016.272.289 .

Nastasijević, Branislav J., Milošević, Maja, Janjić, Goran V., Stanić, Vojislav, Vasić, Vesna M., "Gentiana lutea Extracts and their Constituents as Inhibitors of Synaptosomal Ecto-NTPDase" in International Journal of Pharmacology, 12, no. 4 (2016):272-289,
https://doi.org/10.3923/ijp.2016.272.289 . .

TY  - JOUR
AU  - Petrović, Snježana
AU  - Milošević, Maja
AU  - Ristic-Medic, Danijela
AU  - Velickovic, Natasa
AU  - Drakulić, Dunja R.
AU  - Grković, Ivana
AU  - Horvat, Anica
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/495
AB  - Our earlier studies found that in vitro estradiol modulates mitochondrial Ca2+ transport in discrete brain regions. The present study examined the role of estradiol receptors (ERs) in estradiol-induced inhibition of Ca2+ efflux from synaptosomal mitochondria isolated from rat caudate nuclei and brain stems. Radioactively labeled CaCl2 (0.6-0.75 mu Ci (CaCl2)-Ca-45) was used for Ca2+ transport monitoring. The results revealed that in the presence of ER antagonist 7 alpha, 17 beta-[9[(4,4,5,5,5-pentafluoropentyl) sulfinyl] nonyl] estra-1,3,5( 10)-triene-3,17-diol (ICI 182,780) (1 mu mol/L), the inhibitory effect of estradiol on mitochondrial Ca2+ efflux was more than 60% decreased, suggesting the involvement of ER in this mode of estradiol neuromodulatory action. When particular contributions of ER alpha and ER beta were tested, it was found that ER beta agonist 2,3-bis(4-hydroxy phenyl)-propionitrile (10 nmol/L) inhibited Ca2+ efflux more than 20%, while the inhibition with ER alpha agonist 4,4, 4-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol (10 nmol/L) was about 10%, both compared to the control. Both agonists demonstrated attenuation of Ca2+ efflux decrease in the presence of mitochondrial Na+/Ca2+ exchanger antagonist 7-chloro-5-(2-chlorophenyl)-1,5-dihyhdro-4,1-benzothiazepin-2(3H)-one (10 mu mol/L), showing interference with the inhibitory action of that agent. Our results strongly indicate ERs as the mediators of estradiol-induced mitochondrial Ca2+ efflux inhibition in rat caudate nucleus and brain stem synaptosomes.
T2  - Turkish Journal of Biology
T1  - Estradiol receptors mediate estradiol-induced inhibition of mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem
VL  - 39
IS  - 2
SP  - 328
EP  - 334
DO  - 10.3906/biy-1408-62
ER  - 

@article{
author = "Petrović, Snježana and Milošević, Maja and Ristic-Medic, Danijela and Velickovic, Natasa and Drakulić, Dunja R. and Grković, Ivana and Horvat, Anica",
year = "2015",
abstract = "Our earlier studies found that in vitro estradiol modulates mitochondrial Ca2+ transport in discrete brain regions. The present study examined the role of estradiol receptors (ERs) in estradiol-induced inhibition of Ca2+ efflux from synaptosomal mitochondria isolated from rat caudate nuclei and brain stems. Radioactively labeled CaCl2 (0.6-0.75 mu Ci (CaCl2)-Ca-45) was used for Ca2+ transport monitoring. The results revealed that in the presence of ER antagonist 7 alpha, 17 beta-[9[(4,4,5,5,5-pentafluoropentyl) sulfinyl] nonyl] estra-1,3,5( 10)-triene-3,17-diol (ICI 182,780) (1 mu mol/L), the inhibitory effect of estradiol on mitochondrial Ca2+ efflux was more than 60% decreased, suggesting the involvement of ER in this mode of estradiol neuromodulatory action. When particular contributions of ER alpha and ER beta were tested, it was found that ER beta agonist 2,3-bis(4-hydroxy phenyl)-propionitrile (10 nmol/L) inhibited Ca2+ efflux more than 20%, while the inhibition with ER alpha agonist 4,4, 4-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol (10 nmol/L) was about 10%, both compared to the control. Both agonists demonstrated attenuation of Ca2+ efflux decrease in the presence of mitochondrial Na+/Ca2+ exchanger antagonist 7-chloro-5-(2-chlorophenyl)-1,5-dihyhdro-4,1-benzothiazepin-2(3H)-one (10 mu mol/L), showing interference with the inhibitory action of that agent. Our results strongly indicate ERs as the mediators of estradiol-induced mitochondrial Ca2+ efflux inhibition in rat caudate nucleus and brain stem synaptosomes.",
journal = "Turkish Journal of Biology",
title = "Estradiol receptors mediate estradiol-induced inhibition of mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem",
volume = "39",
number = "2",
pages = "328-334",
doi = "10.3906/biy-1408-62"
}

Petrović, S., Milošević, M., Ristic-Medic, D., Velickovic, N., Drakulić, D. R., Grković, I.,& Horvat, A.. (2015). Estradiol receptors mediate estradiol-induced inhibition of mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem. in Turkish Journal of Biology, 39(2), 328-334.
https://doi.org/10.3906/biy-1408-62

Petrović S, Milošević M, Ristic-Medic D, Velickovic N, Drakulić DR, Grković I, Horvat A. Estradiol receptors mediate estradiol-induced inhibition of mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem. in Turkish Journal of Biology. 2015;39(2):328-334.
doi:10.3906/biy-1408-62 .

Petrović, Snježana, Milošević, Maja, Ristic-Medic, Danijela, Velickovic, Natasa, Drakulić, Dunja R., Grković, Ivana, Horvat, Anica, "Estradiol receptors mediate estradiol-induced inhibition of mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem" in Turkish Journal of Biology, 39, no. 2 (2015):328-334,
https://doi.org/10.3906/biy-1408-62 . .

TY  - JOUR
AU  - Petrovic, S.
AU  - Takic, M.
AU  - Arsic, A.
AU  - Vučić, Vesna
AU  - Drakulić, Dunja R.
AU  - Milošević, Maja
AU  - Glibetić, Marija
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6095
AB  - The effects of 8-days treatment with 17 alpha-estradiol (33.3 mu g/kg) and progesterone (1.7 mg/kg) on plasma lipids and fatty acid composition of plasma phospholipids were examined in intact (INT) and bilaterally common carotid arteries occluded (BCO) male Wistar rats. Significant decrease of triglyceride level was found in BCO rats after the estradiol treatment. Both hormones elevated proportion of 18:1n-7 fatty acid in INT, but they failed to have such an effect in BCO. Estradiol increased 22:5n-3 and total n-3 polyunsaturated fatty acids (PUFA) in intact, and decreased 18:2n-6 in BCO rats. Significantly lower level of total n-3 was found in progesterone-treated than in estradiol-treated BCO rats. Given that n-3 PUFA have many beneficial effects on cell and tissue function, while n-6 PUFA have mostly the opposite effects, estradiol, rather than progesterone, was seen to improve plasma lipids and phospholipids FA profiles in INT and BCO animals. Estradiol significantly elevated the estimated activity of Delta 9-desaturases and progesterone of Delta 5-desaturase in BCO group, with no effects in INT rats.
T2  - Physiological Research
T1  - Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries
VL  - 63
IS  - 3
SP  - 331
EP  - 339
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6095
ER  - 

@article{
author = "Petrovic, S. and Takic, M. and Arsic, A. and Vučić, Vesna and Drakulić, Dunja R. and Milošević, Maja and Glibetić, Marija",
year = "2014",
abstract = "The effects of 8-days treatment with 17 alpha-estradiol (33.3 mu g/kg) and progesterone (1.7 mg/kg) on plasma lipids and fatty acid composition of plasma phospholipids were examined in intact (INT) and bilaterally common carotid arteries occluded (BCO) male Wistar rats. Significant decrease of triglyceride level was found in BCO rats after the estradiol treatment. Both hormones elevated proportion of 18:1n-7 fatty acid in INT, but they failed to have such an effect in BCO. Estradiol increased 22:5n-3 and total n-3 polyunsaturated fatty acids (PUFA) in intact, and decreased 18:2n-6 in BCO rats. Significantly lower level of total n-3 was found in progesterone-treated than in estradiol-treated BCO rats. Given that n-3 PUFA have many beneficial effects on cell and tissue function, while n-6 PUFA have mostly the opposite effects, estradiol, rather than progesterone, was seen to improve plasma lipids and phospholipids FA profiles in INT and BCO animals. Estradiol significantly elevated the estimated activity of Delta 9-desaturases and progesterone of Delta 5-desaturase in BCO group, with no effects in INT rats.",
journal = "Physiological Research",
title = "Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries",
volume = "63",
number = "3",
pages = "331-339",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6095"
}

Petrovic, S., Takic, M., Arsic, A., Vučić, V., Drakulić, D. R., Milošević, M.,& Glibetić, M.. (2014). Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries. in Physiological Research, 63(3), 331-339.
https://hdl.handle.net/21.15107/rcub_vinar_6095

Petrovic S, Takic M, Arsic A, Vučić V, Drakulić DR, Milošević M, Glibetić M. Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries. in Physiological Research. 2014;63(3):331-339.
https://hdl.handle.net/21.15107/rcub_vinar_6095 .

Petrovic, S., Takic, M., Arsic, A., Vučić, Vesna, Drakulić, Dunja R., Milošević, Maja, Glibetić, Marija, "Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries" in Physiological Research, 63, no. 3 (2014):331-339,
https://hdl.handle.net/21.15107/rcub_vinar_6095 .

TY  - CONF
AU  - Cvetićanin, Jelena M.
AU  - Milošević, Maja
AU  - Veličković, Suzana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9180
AB  - The laser desorption/ionization time-of-flight (LDITOF) mass spectra of the
solution of Gd2O3 in HCl are reported. The results presented here illustrate
the usefulness of the linear positive mode of LDI in the characterization of
gadolinium metal ions. In addition, the laser desorption/ionization also leads
to the formation of several ionic species containing gadolinium with oxygen
and chlorine, which were detected in the negative linear mode. The ion
signals are identified by both m/z values and distinctive isotope patterns.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Laser desorption/ionization time-of-flight mass spectra of gadolinium
VL  - B-11-P
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9180
ER  - 

@conference{
author = "Cvetićanin, Jelena M. and Milošević, Maja and Veličković, Suzana",
year = "2014",
abstract = "The laser desorption/ionization time-of-flight (LDITOF) mass spectra of the
solution of Gd2O3 in HCl are reported. The results presented here illustrate
the usefulness of the linear positive mode of LDI in the characterization of
gadolinium metal ions. In addition, the laser desorption/ionization also leads
to the formation of several ionic species containing gadolinium with oxygen
and chlorine, which were detected in the negative linear mode. The ion
signals are identified by both m/z values and distinctive isotope patterns.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Laser desorption/ionization time-of-flight mass spectra of gadolinium",
volume = "B-11-P",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9180"
}

Cvetićanin, J. M., Milošević, M.,& Veličković, S.. (2014). Laser desorption/ionization time-of-flight mass spectra of gadolinium. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., B-11-P.
https://hdl.handle.net/21.15107/rcub_vinar_9180

Cvetićanin JM, Milošević M, Veličković S. Laser desorption/ionization time-of-flight mass spectra of gadolinium. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2014;B-11-P.
https://hdl.handle.net/21.15107/rcub_vinar_9180 .

Cvetićanin, Jelena M., Milošević, Maja, Veličković, Suzana, "Laser desorption/ionization time-of-flight mass spectra of gadolinium" in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry, B-11-P (2014),
https://hdl.handle.net/21.15107/rcub_vinar_9180 .

TY  - CONF
AU  - Pajkić, Borislava
AU  - Milošević, Maja
AU  - Veličković, Suzana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9184
AB  - Lithium heterogeneous clusters were generated from lithium chloride salt using laser desorption/ionization (LDI) on a commercial matrix assisted laser desorption ionization – time of flight (MALDI-TOF) mass spectrometric instrument. The generation of LinCl (n= 2 – 8) clusters, has been successfully achieved in the positive mode when the initial salt concentrations were above approximately 10-3 M and after applying sufficiently high laser energy of 2.3 mW. These clusters can be obtained at higher concentration of LiCl, but the laser energy must be above 3.0 mW. Laser desorption/ionization also leads to the formation of several protonated lithium chloride cluster ions (Li3ClH+ , Li7ClH+), Lin(H2O)2+  and Lin(OH)2+.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Generation of gas-phase lithium chloride cluster ions by laser desorption/ionization mass spectrometry
VL  - B-13-P
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9184
ER  - 

@conference{
author = "Pajkić, Borislava and Milošević, Maja and Veličković, Suzana",
year = "2014",
abstract = "Lithium heterogeneous clusters were generated from lithium chloride salt using laser desorption/ionization (LDI) on a commercial matrix assisted laser desorption ionization – time of flight (MALDI-TOF) mass spectrometric instrument. The generation of LinCl (n= 2 – 8) clusters, has been successfully achieved in the positive mode when the initial salt concentrations were above approximately 10-3 M and after applying sufficiently high laser energy of 2.3 mW. These clusters can be obtained at higher concentration of LiCl, but the laser energy must be above 3.0 mW. Laser desorption/ionization also leads to the formation of several protonated lithium chloride cluster ions (Li3ClH+ , Li7ClH+), Lin(H2O)2+  and Lin(OH)2+.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Generation of gas-phase lithium chloride cluster ions by laser desorption/ionization mass spectrometry",
volume = "B-13-P",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9184"
}

Pajkić, B., Milošević, M.,& Veličković, S.. (2014). Generation of gas-phase lithium chloride cluster ions by laser desorption/ionization mass spectrometry. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., B-13-P.
https://hdl.handle.net/21.15107/rcub_vinar_9184

Pajkić B, Milošević M, Veličković S. Generation of gas-phase lithium chloride cluster ions by laser desorption/ionization mass spectrometry. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2014;B-13-P.
https://hdl.handle.net/21.15107/rcub_vinar_9184 .

Pajkić, Borislava, Milošević, Maja, Veličković, Suzana, "Generation of gas-phase lithium chloride cluster ions by laser desorption/ionization mass spectrometry" in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry, B-13-P (2014),
https://hdl.handle.net/21.15107/rcub_vinar_9184 .

TY  - JOUR
AU  - Velickovic, Natasa A.
AU  - Đorđević, Ana D.
AU  - Drakulić, Dunja R.
AU  - Šećerov, Bojana Lj.
AU  - Grković, Ivana
AU  - Milošević, Maja
AU  - Horvat, Anica
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5594
AB  - Ionizing radiation is commonly used in the treatment of brain tumors but it can impair cognitive functions, such as learning and memory. Since cognitive dysfunctions are predominantly result of cell death by apoptosis in hippocampal cells, in this study we analyzed acute effects of cranial gamma-irradiation (10 Gy) on-expression-of proapoptotic molecules (p53, Box) and antiapoptotic molecule Bcl-2, as well as caspase-3 activation and cytochrome c redistribution in the hippocampus of young rats. The selected regimen of irradiation resembles the established animal model for childhood prophylactic cranial radiotherapy. Our results demonstrated that p53 mRNA expression was unchanged after irradiation, while induction of p53 protein was rapid. In parallel, Bax mRNA and protein levels were also increased following irradiation, whereas Bcl-2 expression was not changed during the examined post-irradiation period. These changes were accompanied with early hallmarks of apoptosis, such as increased cytochrome c release and stimulated activation of caspase-3. Overall, this study demonstrates that cranial irradiation is associated with the augmented apoptotic pathway in the rat hippocampus, which could be related to the cognitive decline observed in patients after prophylactic cranial radiotherapy, but also opens perspective in finding radioprotectors that can mitigate radiation injury of normal brain tissue.
T2  - Nuclear technology and radiation protection
T1  - Radiation-Mediated Induction of Apoptotic Cell Death in Rat Hippocampus
VL  - 28
IS  - 2
SP  - 212
EP  - 220
DO  - 10.2298/NTRP1302212V
ER  - 

@article{
author = "Velickovic, Natasa A. and Đorđević, Ana D. and Drakulić, Dunja R. and Šećerov, Bojana Lj. and Grković, Ivana and Milošević, Maja and Horvat, Anica",
year = "2013",
abstract = "Ionizing radiation is commonly used in the treatment of brain tumors but it can impair cognitive functions, such as learning and memory. Since cognitive dysfunctions are predominantly result of cell death by apoptosis in hippocampal cells, in this study we analyzed acute effects of cranial gamma-irradiation (10 Gy) on-expression-of proapoptotic molecules (p53, Box) and antiapoptotic molecule Bcl-2, as well as caspase-3 activation and cytochrome c redistribution in the hippocampus of young rats. The selected regimen of irradiation resembles the established animal model for childhood prophylactic cranial radiotherapy. Our results demonstrated that p53 mRNA expression was unchanged after irradiation, while induction of p53 protein was rapid. In parallel, Bax mRNA and protein levels were also increased following irradiation, whereas Bcl-2 expression was not changed during the examined post-irradiation period. These changes were accompanied with early hallmarks of apoptosis, such as increased cytochrome c release and stimulated activation of caspase-3. Overall, this study demonstrates that cranial irradiation is associated with the augmented apoptotic pathway in the rat hippocampus, which could be related to the cognitive decline observed in patients after prophylactic cranial radiotherapy, but also opens perspective in finding radioprotectors that can mitigate radiation injury of normal brain tissue.",
journal = "Nuclear technology and radiation protection",
title = "Radiation-Mediated Induction of Apoptotic Cell Death in Rat Hippocampus",
volume = "28",
number = "2",
pages = "212-220",
doi = "10.2298/NTRP1302212V"
}

Velickovic, N. A., Đorđević, A. D., Drakulić, D. R., Šećerov, B. Lj., Grković, I., Milošević, M.,& Horvat, A.. (2013). Radiation-Mediated Induction of Apoptotic Cell Death in Rat Hippocampus. in Nuclear technology and radiation protection, 28(2), 212-220.
https://doi.org/10.2298/NTRP1302212V

Velickovic NA, Đorđević AD, Drakulić DR, Šećerov BL, Grković I, Milošević M, Horvat A. Radiation-Mediated Induction of Apoptotic Cell Death in Rat Hippocampus. in Nuclear technology and radiation protection. 2013;28(2):212-220.
doi:10.2298/NTRP1302212V .

Velickovic, Natasa A., Đorđević, Ana D., Drakulić, Dunja R., Šećerov, Bojana Lj., Grković, Ivana, Milošević, Maja, Horvat, Anica, "Radiation-Mediated Induction of Apoptotic Cell Death in Rat Hippocampus" in Nuclear technology and radiation protection, 28, no. 2 (2013):212-220,
https://doi.org/10.2298/NTRP1302212V . .

TY  - JOUR
AU  - Petrović, Snježana
AU  - Milošević, Maja
AU  - Drakulić, Dunja R.
AU  - Grković, Ivana
AU  - Stanojlović, Miloš R.
AU  - Mitrović, Nataša Lj.
AU  - Horvat, Anica
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5012
AB  - The aim of this study was to examine the rapid non-genomic effect of 17 beta-estradiol (E2) on Ca2+ transport in mitochondria isolated from the nerve terminals (synaptosomes) of caudate nuclei (NC) and brain stems (BS) of ovariectomised female rats. In physiological conditions no effect of E2 on Ca2+ influx into synaptosomal mitochondria through ruthenium red (RR)-sensitive uniporter was observed. However, in the presence of uncoupling agent carbonyl cyanide4-(trifluoromethoxy)phenylhydrazone (FCCP) (1 mu mol/l), pre-treatment with 0.5 nmol/l E2 protected mitochondrial membrane potential and consequently increased Ca2+ influx (2.3-fold in NC and 3.1-fold in BS). At the same time, 0.5 nmol/l E2 by increasing the affinity of mitochondrial Na+/Ca2+ exchanger for Na+ inhibited mitochondrial Ca2+ efflux in NC and BS by about 40%. Also, the specific binding of physiological E2 concentrations (0.1-10 nmol/l) to isolated synaptosomal mitochondria was detected. Using membrane impermeable E2 bound to bovine serum albumin and selective inhibitor of mitochondrial Na+/Ca2+ exchanger, we obtained that E2s action on mitochondrial Ca2+ efflux at least partially is due to the direct effects on the mitochondrial membrane and/or Na+/Ca2+ exchanger located in inner mitochondrial membrane. Our results implicate E2 as a modulator of Ca2+ concentration in mitochondrial matrix, and ultimately in the cytosol. Given the vital role of Ca2+ in regulation of total nerve cells activity, especially energy metabolism, neurotransmission and directing the cells toward survival or cell death, the effects on mitochondrial Ca2+ transport could be one of the important modes of E2 neuromodulatory action independent of the genome. (C) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.
T2  - Neuroscience
T1  - 17 beta-estradiol modulates mitochondrial Ca2+ flux in rat caudate nucleus and brain stem
VL  - 220
SP  - 32
EP  - 40
DO  - 10.1016/j.neuroscience.2012.06.040
ER  - 

@article{
author = "Petrović, Snježana and Milošević, Maja and Drakulić, Dunja R. and Grković, Ivana and Stanojlović, Miloš R. and Mitrović, Nataša Lj. and Horvat, Anica",
year = "2012",
abstract = "The aim of this study was to examine the rapid non-genomic effect of 17 beta-estradiol (E2) on Ca2+ transport in mitochondria isolated from the nerve terminals (synaptosomes) of caudate nuclei (NC) and brain stems (BS) of ovariectomised female rats. In physiological conditions no effect of E2 on Ca2+ influx into synaptosomal mitochondria through ruthenium red (RR)-sensitive uniporter was observed. However, in the presence of uncoupling agent carbonyl cyanide4-(trifluoromethoxy)phenylhydrazone (FCCP) (1 mu mol/l), pre-treatment with 0.5 nmol/l E2 protected mitochondrial membrane potential and consequently increased Ca2+ influx (2.3-fold in NC and 3.1-fold in BS). At the same time, 0.5 nmol/l E2 by increasing the affinity of mitochondrial Na+/Ca2+ exchanger for Na+ inhibited mitochondrial Ca2+ efflux in NC and BS by about 40%. Also, the specific binding of physiological E2 concentrations (0.1-10 nmol/l) to isolated synaptosomal mitochondria was detected. Using membrane impermeable E2 bound to bovine serum albumin and selective inhibitor of mitochondrial Na+/Ca2+ exchanger, we obtained that E2s action on mitochondrial Ca2+ efflux at least partially is due to the direct effects on the mitochondrial membrane and/or Na+/Ca2+ exchanger located in inner mitochondrial membrane. Our results implicate E2 as a modulator of Ca2+ concentration in mitochondrial matrix, and ultimately in the cytosol. Given the vital role of Ca2+ in regulation of total nerve cells activity, especially energy metabolism, neurotransmission and directing the cells toward survival or cell death, the effects on mitochondrial Ca2+ transport could be one of the important modes of E2 neuromodulatory action independent of the genome. (C) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.",
journal = "Neuroscience",
title = "17 beta-estradiol modulates mitochondrial Ca2+ flux in rat caudate nucleus and brain stem",
volume = "220",
pages = "32-40",
doi = "10.1016/j.neuroscience.2012.06.040"
}

Petrović, S., Milošević, M., Drakulić, D. R., Grković, I., Stanojlović, M. R., Mitrović, N. Lj.,& Horvat, A.. (2012). 17 beta-estradiol modulates mitochondrial Ca2+ flux in rat caudate nucleus and brain stem. in Neuroscience, 220, 32-40.
https://doi.org/10.1016/j.neuroscience.2012.06.040

Petrović S, Milošević M, Drakulić DR, Grković I, Stanojlović MR, Mitrović NL, Horvat A. 17 beta-estradiol modulates mitochondrial Ca2+ flux in rat caudate nucleus and brain stem. in Neuroscience. 2012;220:32-40.
doi:10.1016/j.neuroscience.2012.06.040 .

Petrović, Snježana, Milošević, Maja, Drakulić, Dunja R., Grković, Ivana, Stanojlović, Miloš R., Mitrović, Nataša Lj., Horvat, Anica, "17 beta-estradiol modulates mitochondrial Ca2+ flux in rat caudate nucleus and brain stem" in Neuroscience, 220 (2012):32-40,
https://doi.org/10.1016/j.neuroscience.2012.06.040 . .

TY  - JOUR
AU  - Velickovic, Natasa
AU  - Drakulić, Dunja R.
AU  - Petrovic, Snjezana
AU  - Grković, Ivana
AU  - Milošević, Maja
AU  - Stanojlović, Miloš R.
AU  - Horvat, Anica
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5051
AB  - Recent studies reported that exposure of juvenile rats to cranial irradiation affects hypothalamic-pituitary-adrenal (HPA) axis stability, leading to its activation along with radiation-induced inflammation. In the present study, we hypothesized whether inflammatory reaction in the CNS could be a mediator of HPA axis response to cranial irradiation (CI). Therefore, we analyzed time-course changes of serum corticosterone level, as well IL-1 beta and TNF-alpha level in the serum and hypothalamus of juvenile rats after CI. Protein and gene expression of the glucocorticoid receptor (GR) and nuclear factor kappaB (NF kappa B) were examined in the hippocampus within 24 h postirradiation interval. Cranial irradiation led to rapid induction of both GR and NF kappa B mRNA and protein in the hippocampus at 1 h. The increment in NF kappa B protein persisted for 2 h, therefore NF kappa B/GR protein ratio was turned in favor of NF kappa B. Central inflammation was characterized by increased IL-1 beta in the hypothalamus, with maximum levels at 2 and 4 h after irradiation, while both IL-1 beta and TNF-alpha were undetectable in the serum. Enhanced hypothalamic IL-1 beta probably induced the relocation of hippocampal NF kappa B to the nucleus and decreased NF kappa B mRNA at 6 h, indicating promotion of inflammation in the key tissue for HPA axis regulation. Concomitant increase of corticosterone level and enhanced GR nuclear translocation in the hippocampus at 6 h might represent a compensatory mechanism for observed inflammation. Our results indicate that acute radiation response is characterized by increased central inflammation and concomitant HPA axis activation, most likely having a role in protection of the organism from overwhelming inflammatory reaction.
T2  - Cellular and Molecular Neurobiology
T1  - Time-Course of Hypothalamic-Pituitary-Adrenal Axis Activity and Inflammation in Juvenile Rat Brain After Cranial Irradiation
VL  - 32
IS  - 7
SP  - 1175
EP  - 1185
DO  - 10.1007/s10571-012-9843-1
ER  - 

@article{
author = "Velickovic, Natasa and Drakulić, Dunja R. and Petrovic, Snjezana and Grković, Ivana and Milošević, Maja and Stanojlović, Miloš R. and Horvat, Anica",
year = "2012",
abstract = "Recent studies reported that exposure of juvenile rats to cranial irradiation affects hypothalamic-pituitary-adrenal (HPA) axis stability, leading to its activation along with radiation-induced inflammation. In the present study, we hypothesized whether inflammatory reaction in the CNS could be a mediator of HPA axis response to cranial irradiation (CI). Therefore, we analyzed time-course changes of serum corticosterone level, as well IL-1 beta and TNF-alpha level in the serum and hypothalamus of juvenile rats after CI. Protein and gene expression of the glucocorticoid receptor (GR) and nuclear factor kappaB (NF kappa B) were examined in the hippocampus within 24 h postirradiation interval. Cranial irradiation led to rapid induction of both GR and NF kappa B mRNA and protein in the hippocampus at 1 h. The increment in NF kappa B protein persisted for 2 h, therefore NF kappa B/GR protein ratio was turned in favor of NF kappa B. Central inflammation was characterized by increased IL-1 beta in the hypothalamus, with maximum levels at 2 and 4 h after irradiation, while both IL-1 beta and TNF-alpha were undetectable in the serum. Enhanced hypothalamic IL-1 beta probably induced the relocation of hippocampal NF kappa B to the nucleus and decreased NF kappa B mRNA at 6 h, indicating promotion of inflammation in the key tissue for HPA axis regulation. Concomitant increase of corticosterone level and enhanced GR nuclear translocation in the hippocampus at 6 h might represent a compensatory mechanism for observed inflammation. Our results indicate that acute radiation response is characterized by increased central inflammation and concomitant HPA axis activation, most likely having a role in protection of the organism from overwhelming inflammatory reaction.",
journal = "Cellular and Molecular Neurobiology",
title = "Time-Course of Hypothalamic-Pituitary-Adrenal Axis Activity and Inflammation in Juvenile Rat Brain After Cranial Irradiation",
volume = "32",
number = "7",
pages = "1175-1185",
doi = "10.1007/s10571-012-9843-1"
}

Velickovic, N., Drakulić, D. R., Petrovic, S., Grković, I., Milošević, M., Stanojlović, M. R.,& Horvat, A.. (2012). Time-Course of Hypothalamic-Pituitary-Adrenal Axis Activity and Inflammation in Juvenile Rat Brain After Cranial Irradiation. in Cellular and Molecular Neurobiology, 32(7), 1175-1185.
https://doi.org/10.1007/s10571-012-9843-1

Velickovic N, Drakulić DR, Petrovic S, Grković I, Milošević M, Stanojlović MR, Horvat A. Time-Course of Hypothalamic-Pituitary-Adrenal Axis Activity and Inflammation in Juvenile Rat Brain After Cranial Irradiation. in Cellular and Molecular Neurobiology. 2012;32(7):1175-1185.
doi:10.1007/s10571-012-9843-1 .

Velickovic, Natasa, Drakulić, Dunja R., Petrovic, Snjezana, Grković, Ivana, Milošević, Maja, Stanojlović, Miloš R., Horvat, Anica, "Time-Course of Hypothalamic-Pituitary-Adrenal Axis Activity and Inflammation in Juvenile Rat Brain After Cranial Irradiation" in Cellular and Molecular Neurobiology, 32, no. 7 (2012):1175-1185,
https://doi.org/10.1007/s10571-012-9843-1 . .

TY  - JOUR
AU  - Milošević, Maja
AU  - Petrovic, Snjezana
AU  - Velickovic, Natasa
AU  - Grković, Ivana
AU  - Ignjatović, Marija
AU  - Horvat, Anica
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5131
AB  - Extracellular nucleotides affect female reproductive functions, fertilization, and pregnancy. The aim of this study was to investigate biochemical characteristics of ATP and ADP hydrolysis and identify E-NTPDases in myometrial cell membranes from Wistar albino rats. The apparent K (m) values were 506.4 +/- A 62.1 and 638.8 +/- A 31.3 mu M, with a calculated V (max) (app) of 3,973.0 +/- A 279.5 and 2,853.9 +/- A 79.8 nmol/min/mg for ATP and ADP, respectively. The enzyme activity described here has common properties characteristic for NTPDases: divalent cation dependence; alkaline pH optimum for both substrates, insensitivity to some of classical ATPase inhibitors (ouabain, oligomycine, theophylline, levamisole) and significant inhibition by suramine and high concentration of sodium azides (5 mM). According to similar apparent K-m values for both substrates, the ATP/ADP hydrolysis ratio, and Chevillard competition plot, NTPDase1 is dominant ATP/ADP hydrolyzing enzyme in myometrial cell membranes. RT-PCR analysis revealed expression of three members of ectonucleoside triphosphate diphosphohydrolase family (NTPDase 1, 2, and 8) in rat uterus. These findings may further elucidate the role of NTPDases and ATP in reproductive physiology.
T2  - Molecular and Cellular Biochemistry
T1  - ATP and ADP hydrolysis in cell membranes from rat myometrium
VL  - 371
IS  - 1-2
SP  - 199
EP  - 208
DO  - 10.1007/s11010-012-1436-2
ER  - 

@article{
author = "Milošević, Maja and Petrovic, Snjezana and Velickovic, Natasa and Grković, Ivana and Ignjatović, Marija and Horvat, Anica",
year = "2012",
abstract = "Extracellular nucleotides affect female reproductive functions, fertilization, and pregnancy. The aim of this study was to investigate biochemical characteristics of ATP and ADP hydrolysis and identify E-NTPDases in myometrial cell membranes from Wistar albino rats. The apparent K (m) values were 506.4 +/- A 62.1 and 638.8 +/- A 31.3 mu M, with a calculated V (max) (app) of 3,973.0 +/- A 279.5 and 2,853.9 +/- A 79.8 nmol/min/mg for ATP and ADP, respectively. The enzyme activity described here has common properties characteristic for NTPDases: divalent cation dependence; alkaline pH optimum for both substrates, insensitivity to some of classical ATPase inhibitors (ouabain, oligomycine, theophylline, levamisole) and significant inhibition by suramine and high concentration of sodium azides (5 mM). According to similar apparent K-m values for both substrates, the ATP/ADP hydrolysis ratio, and Chevillard competition plot, NTPDase1 is dominant ATP/ADP hydrolyzing enzyme in myometrial cell membranes. RT-PCR analysis revealed expression of three members of ectonucleoside triphosphate diphosphohydrolase family (NTPDase 1, 2, and 8) in rat uterus. These findings may further elucidate the role of NTPDases and ATP in reproductive physiology.",
journal = "Molecular and Cellular Biochemistry",
title = "ATP and ADP hydrolysis in cell membranes from rat myometrium",
volume = "371",
number = "1-2",
pages = "199-208",
doi = "10.1007/s11010-012-1436-2"
}

Milošević, M., Petrovic, S., Velickovic, N., Grković, I., Ignjatović, M.,& Horvat, A.. (2012). ATP and ADP hydrolysis in cell membranes from rat myometrium. in Molecular and Cellular Biochemistry, 371(1-2), 199-208.
https://doi.org/10.1007/s11010-012-1436-2

Milošević M, Petrovic S, Velickovic N, Grković I, Ignjatović M, Horvat A. ATP and ADP hydrolysis in cell membranes from rat myometrium. in Molecular and Cellular Biochemistry. 2012;371(1-2):199-208.
doi:10.1007/s11010-012-1436-2 .

Milošević, Maja, Petrovic, Snjezana, Velickovic, Natasa, Grković, Ivana, Ignjatović, Marija, Horvat, Anica, "ATP and ADP hydrolysis in cell membranes from rat myometrium" in Molecular and Cellular Biochemistry, 371, no. 1-2 (2012):199-208,
https://doi.org/10.1007/s11010-012-1436-2 . .

TY  - CONF
AU  - Drakulić, Dunja R.
AU  - Grković, Ivana
AU  - Milošević, Maja
AU  - Petrović, Snježana
AU  - Stanojlović, Miloš R.
AU  - Mitrović, Nataša Lj.
AU  - Horvat, Anica
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9295
AB  - The present study was performed to investigate whether acute whole-body exposure of
female adult rats to low dose (0.5 Gy) of ionizing irradiation (IR) is sufficient to alter
ectonucleotidase enzyme activities in the brain. All measurements were done at time
points 1, 24 and 72h after irradiation. Neuronal synaptic plasma membranes (SPMs)
were isolated from whole brains and enzyme activities were determined by monitoring
ATP, ADP and AMP hydrolysis in vitro. Our results indicate that whole-body IR is
able to modulate investigated brain enzyme activities in a time-dependent manner.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
T1  - Radiation-mediated modulations of extracelluar nucleotide hydrolysis in adult female rat brain
VL  - 1
SP  - 373
EP  - 375
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9295
ER  - 

@conference{
author = "Drakulić, Dunja R. and Grković, Ivana and Milošević, Maja and Petrović, Snježana and Stanojlović, Miloš R. and Mitrović, Nataša Lj. and Horvat, Anica",
year = "2012",
abstract = "The present study was performed to investigate whether acute whole-body exposure of
female adult rats to low dose (0.5 Gy) of ionizing irradiation (IR) is sufficient to alter
ectonucleotidase enzyme activities in the brain. All measurements were done at time
points 1, 24 and 72h after irradiation. Neuronal synaptic plasma membranes (SPMs)
were isolated from whole brains and enzyme activities were determined by monitoring
ATP, ADP and AMP hydrolysis in vitro. Our results indicate that whole-body IR is
able to modulate investigated brain enzyme activities in a time-dependent manner.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry",
title = "Radiation-mediated modulations of extracelluar nucleotide hydrolysis in adult female rat brain",
volume = "1",
pages = "373-375",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9295"
}

Drakulić, D. R., Grković, I., Milošević, M., Petrović, S., Stanojlović, M. R., Mitrović, N. Lj.,& Horvat, A.. (2012). Radiation-mediated modulations of extracelluar nucleotide hydrolysis in adult female rat brain. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 373-375.
https://hdl.handle.net/21.15107/rcub_vinar_9295

Drakulić DR, Grković I, Milošević M, Petrović S, Stanojlović MR, Mitrović NL, Horvat A. Radiation-mediated modulations of extracelluar nucleotide hydrolysis in adult female rat brain. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry. 2012;1:373-375.
https://hdl.handle.net/21.15107/rcub_vinar_9295 .

Drakulić, Dunja R., Grković, Ivana, Milošević, Maja, Petrović, Snježana, Stanojlović, Miloš R., Mitrović, Nataša Lj., Horvat, Anica, "Radiation-mediated modulations of extracelluar nucleotide hydrolysis in adult female rat brain" in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry, 1 (2012):373-375,
https://hdl.handle.net/21.15107/rcub_vinar_9295 .

TY  - JOUR
AU  - Petrović, S.
AU  - Velickovic, N.
AU  - Stanojević, Ivana
AU  - Milošević, Maja
AU  - Drakulić, Dunja R.
AU  - Stanojlović, Miloš R.
AU  - Horvat, Anica
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4512
AB  - Our results, as well as those of others, have indicated that 17 beta-estradiol (E2) exerts its nongenomic effects in neuronal cells by affecting plasma membrane Ca2+ flux. In neuronal cells mitochondria possess Ca2+ buffering properties as they both sequester and release Ca2+. The goal of this study was to examine the rapid non-genomic effect of E2 on mitochondria! Ca2+ transport in hippocampal synaptosomes from ovariectomised rats. In addition, we aimed to determine if, and to what extent, E2 receptors participated in mitochondria! Ca2+ transport modulation by E2 in vitro. E2-specific binding and Ca2+ transport was monitored. At physiological E2 concentrations (0.1-1.5 nmol/L), specific E2 binding to mitochondria isolated from hippocampal synaptosomes was detected with a B-max and K-m of 37.6 +/- 2.6 fmol/mg protein and 0.69 +/- 0.14 nmol/L of free E2, respectively. The main mitochondrial Ca2+ influx mechanism is the Ruthenium Red-sensitive uniporter driven by mitochondrial membrane potential. Despite no effect of E2 on Ca2+ influx, a physiological E2 concentration (0.5 nmol/L) protected mitochondrial membrane potential and consequently Ca2+ influx from the uncoupling agent carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (1 mu mol/L). In neuronal cells the predominant mitochondria! Ca2+ efflux mechanism is the Na+/Ca2+ exchanger. E2 caused Ca2+ efflux inhibition (by 46%) coupled with increased affinity of the Na+/Ca2+ exchanger for Na+. Using E2 receptor (ER alpha and ER beta) antagonists and agonists, we confirmed ER betas involvement in E2-induced mitochondrial membrane potential protection as well as Ca2+ efflux inhibition. In summary, our results indicate that the nongenomic neuromodulatory role of E2 in rat hippocampus is achieved by affecting mitochondria! Ca2+ transport via, in part, mitochondrial ER beta. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.
T2  - Neuroscience
T1  - Inhibition of Mitochondrial Na+-Dependent Ca2+ Efflux By 17 Beta-Estradiol in the Rat Hippocampus
VL  - 192
SP  - 195
EP  - 204
DO  - 10.1016/j.neuroscience.2011.06.030
ER  - 

@article{
author = "Petrović, S. and Velickovic, N. and Stanojević, Ivana and Milošević, Maja and Drakulić, Dunja R. and Stanojlović, Miloš R. and Horvat, Anica",
year = "2011",
abstract = "Our results, as well as those of others, have indicated that 17 beta-estradiol (E2) exerts its nongenomic effects in neuronal cells by affecting plasma membrane Ca2+ flux. In neuronal cells mitochondria possess Ca2+ buffering properties as they both sequester and release Ca2+. The goal of this study was to examine the rapid non-genomic effect of E2 on mitochondria! Ca2+ transport in hippocampal synaptosomes from ovariectomised rats. In addition, we aimed to determine if, and to what extent, E2 receptors participated in mitochondria! Ca2+ transport modulation by E2 in vitro. E2-specific binding and Ca2+ transport was monitored. At physiological E2 concentrations (0.1-1.5 nmol/L), specific E2 binding to mitochondria isolated from hippocampal synaptosomes was detected with a B-max and K-m of 37.6 +/- 2.6 fmol/mg protein and 0.69 +/- 0.14 nmol/L of free E2, respectively. The main mitochondrial Ca2+ influx mechanism is the Ruthenium Red-sensitive uniporter driven by mitochondrial membrane potential. Despite no effect of E2 on Ca2+ influx, a physiological E2 concentration (0.5 nmol/L) protected mitochondrial membrane potential and consequently Ca2+ influx from the uncoupling agent carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (1 mu mol/L). In neuronal cells the predominant mitochondria! Ca2+ efflux mechanism is the Na+/Ca2+ exchanger. E2 caused Ca2+ efflux inhibition (by 46%) coupled with increased affinity of the Na+/Ca2+ exchanger for Na+. Using E2 receptor (ER alpha and ER beta) antagonists and agonists, we confirmed ER betas involvement in E2-induced mitochondrial membrane potential protection as well as Ca2+ efflux inhibition. In summary, our results indicate that the nongenomic neuromodulatory role of E2 in rat hippocampus is achieved by affecting mitochondria! Ca2+ transport via, in part, mitochondrial ER beta. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.",
journal = "Neuroscience",
title = "Inhibition of Mitochondrial Na+-Dependent Ca2+ Efflux By 17 Beta-Estradiol in the Rat Hippocampus",
volume = "192",
pages = "195-204",
doi = "10.1016/j.neuroscience.2011.06.030"
}

Petrović, S., Velickovic, N., Stanojević, I., Milošević, M., Drakulić, D. R., Stanojlović, M. R.,& Horvat, A.. (2011). Inhibition of Mitochondrial Na+-Dependent Ca2+ Efflux By 17 Beta-Estradiol in the Rat Hippocampus. in Neuroscience, 192, 195-204.
https://doi.org/10.1016/j.neuroscience.2011.06.030

Petrović S, Velickovic N, Stanojević I, Milošević M, Drakulić DR, Stanojlović MR, Horvat A. Inhibition of Mitochondrial Na+-Dependent Ca2+ Efflux By 17 Beta-Estradiol in the Rat Hippocampus. in Neuroscience. 2011;192:195-204.
doi:10.1016/j.neuroscience.2011.06.030 .

Petrović, S., Velickovic, N., Stanojević, Ivana, Milošević, Maja, Drakulić, Dunja R., Stanojlović, Miloš R., Horvat, Anica, "Inhibition of Mitochondrial Na+-Dependent Ca2+ Efflux By 17 Beta-Estradiol in the Rat Hippocampus" in Neuroscience, 192 (2011):195-204,
https://doi.org/10.1016/j.neuroscience.2011.06.030 . .

TY  - JOUR
AU  - Stanojević, Ivana
AU  - Drakulić, Dunja R.
AU  - Petrovic, S.
AU  - Milošević, Maja
AU  - Jovanović, N.
AU  - Horvat, Anica
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4603
AB  - A family of enzymes named ecto-nucleoside triphosphate diphosphohydrolase (NTPDases) catalyzes the termination of ATP and ADP actions. Three different NTPDases (NTPDase 1-3), differing in their preference for a substrate, have been localized in the brain of adult mammals. The goal of our study was to clarify ATP and ADP hydrolyzing activities and kinetic parameters of NTPDases in synaptic plasma membranes (SPM) isolated from 15-, 30-, 60- and 90-days-old female rat brains. ATP and ADP hydrolysis were maximal in the presence of Mg(2+) and showed insensitivity to ion-transporting ATPase inhibitors. The pronounced increase in both, ATP and ADP hydrolysis, were found in the SPM isolated from rats in the first month of life, stayed at the same level in the second month, and then decreased in adulthood. Kinetic analysis are also developmental-dependent, and together with the rate of ATP:ADP hydrolysis, point that all three NTPDases are present in SPM isolated from different developmental stages, with different, developmental-dependent proportion of activities. The lowest velocity and the highest affinity were observed for ATP hydrolyses, while the highest velocity and lowest affinity were detected for ADP hydrolyses in SPM isolated from 15-day old rats. Since specific ATP and ADP hydrolysis were lowest in this stage, we concluded that velocity is crucial for ATPase-, while affinity is for ADPase-part of NTPDases. Increased NTPDases activities, changes in their hydrolysis velocity and substrates affinities during rat postnatal development indicate involvement of adenine nucleotides in processes implicated to neuronal maturation and augmented neuroprotection.
T2  - Russian Journal of Physical Chemistry A
T1  - Kinetic characterization of ecto-nucleoside triphosphate diphosphohydrolases in brain nerve terminals during rat postnatal development
VL  - 85
IS  - 13
SP  - 2416
EP  - 2421
DO  - 10.1134/S0036024411130292
ER  - 

@article{
author = "Stanojević, Ivana and Drakulić, Dunja R. and Petrovic, S. and Milošević, Maja and Jovanović, N. and Horvat, Anica",
year = "2011",
abstract = "A family of enzymes named ecto-nucleoside triphosphate diphosphohydrolase (NTPDases) catalyzes the termination of ATP and ADP actions. Three different NTPDases (NTPDase 1-3), differing in their preference for a substrate, have been localized in the brain of adult mammals. The goal of our study was to clarify ATP and ADP hydrolyzing activities and kinetic parameters of NTPDases in synaptic plasma membranes (SPM) isolated from 15-, 30-, 60- and 90-days-old female rat brains. ATP and ADP hydrolysis were maximal in the presence of Mg(2+) and showed insensitivity to ion-transporting ATPase inhibitors. The pronounced increase in both, ATP and ADP hydrolysis, were found in the SPM isolated from rats in the first month of life, stayed at the same level in the second month, and then decreased in adulthood. Kinetic analysis are also developmental-dependent, and together with the rate of ATP:ADP hydrolysis, point that all three NTPDases are present in SPM isolated from different developmental stages, with different, developmental-dependent proportion of activities. The lowest velocity and the highest affinity were observed for ATP hydrolyses, while the highest velocity and lowest affinity were detected for ADP hydrolyses in SPM isolated from 15-day old rats. Since specific ATP and ADP hydrolysis were lowest in this stage, we concluded that velocity is crucial for ATPase-, while affinity is for ADPase-part of NTPDases. Increased NTPDases activities, changes in their hydrolysis velocity and substrates affinities during rat postnatal development indicate involvement of adenine nucleotides in processes implicated to neuronal maturation and augmented neuroprotection.",
journal = "Russian Journal of Physical Chemistry A",
title = "Kinetic characterization of ecto-nucleoside triphosphate diphosphohydrolases in brain nerve terminals during rat postnatal development",
volume = "85",
number = "13",
pages = "2416-2421",
doi = "10.1134/S0036024411130292"
}

Stanojević, I., Drakulić, D. R., Petrovic, S., Milošević, M., Jovanović, N.,& Horvat, A.. (2011). Kinetic characterization of ecto-nucleoside triphosphate diphosphohydrolases in brain nerve terminals during rat postnatal development. in Russian Journal of Physical Chemistry A, 85(13), 2416-2421.
https://doi.org/10.1134/S0036024411130292

Stanojević I, Drakulić DR, Petrovic S, Milošević M, Jovanović N, Horvat A. Kinetic characterization of ecto-nucleoside triphosphate diphosphohydrolases in brain nerve terminals during rat postnatal development. in Russian Journal of Physical Chemistry A. 2011;85(13):2416-2421.
doi:10.1134/S0036024411130292 .

Stanojević, Ivana, Drakulić, Dunja R., Petrovic, S., Milošević, Maja, Jovanović, N., Horvat, Anica, "Kinetic characterization of ecto-nucleoside triphosphate diphosphohydrolases in brain nerve terminals during rat postnatal development" in Russian Journal of Physical Chemistry A, 85, no. 13 (2011):2416-2421,
https://doi.org/10.1134/S0036024411130292 . .

TY  - JOUR
AU  - Horvat, Anica
AU  - Stanojević, Ivana
AU  - Drakulić, Dunja R.
AU  - Velickovic, Natasa
AU  - Petrović, Snježana
AU  - Milošević, Maja
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3941
AB  - The aim of the present study was to examine the effect of acute restraint stress on rat brain synaptosomal plasma membrane (SPM) ecto-nucleotidase activities at specific stages of postnatal development (15-, 30-, 60- and 90-day-old rats) by measuring the rates of ATP, ADP and AMP hydrolysis 1,24 and 72 h post-stress. At 1 h after stress NTPDase and ecto-5-nucleotidase activities were decreased in rats aged up to 60 days old. In adult rats elevated enzyme activities were detected, which indicated the existence of different short-term stress responses during development. A similar pattern of ATP and ADP hydrolysis changes as well as the ATP/ADP ratio in all developmental stages indicated that NTPDase3 was acutely affected after stress. The long-term effect of acute stress on NTPDase activity differed during postnatal development. In juvenile animals (15 days old) NTPDase activity was not altered. However, in later developmental stages (30 and 60 days old rats) NTPDase activity decreased and persisted for 72 h post-stress. In adult rats only ATP hydrolysis was decreased after 24 h, indicating that ecto-ATPase was affected by stress. Ecto-5-nucleotidase hydrolysing activity was decreased within 24 h in adult rats, while in 15- and 30-day old rats it decreased 72 h post-stress. At equivalent times in pubertal rats (60 days old) a slight activation of ecto-5-nucleotidase was detected. Our results highlight the developmental-dependence of brain ecto-nucleotidase susceptibility to acute stress and the likely existence of different mechanisms involved in time-dependent ecto-nucleotidase activity modulation following stress exposure. Clearly there are differences in the response of the purinergic system to acute restraint stress between young and adult rats. (C) 2009 ISDN. Published by Elsevier Ltd. All rights reserved.
T2  - International Journal of Developmental Neuroscience
T1  - Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development
VL  - 28
IS  - 2
SP  - 175
EP  - 182
DO  - 10.1016/j.ijdevneu.2009.11.005
ER  - 

@article{
author = "Horvat, Anica and Stanojević, Ivana and Drakulić, Dunja R. and Velickovic, Natasa and Petrović, Snježana and Milošević, Maja",
year = "2010",
abstract = "The aim of the present study was to examine the effect of acute restraint stress on rat brain synaptosomal plasma membrane (SPM) ecto-nucleotidase activities at specific stages of postnatal development (15-, 30-, 60- and 90-day-old rats) by measuring the rates of ATP, ADP and AMP hydrolysis 1,24 and 72 h post-stress. At 1 h after stress NTPDase and ecto-5-nucleotidase activities were decreased in rats aged up to 60 days old. In adult rats elevated enzyme activities were detected, which indicated the existence of different short-term stress responses during development. A similar pattern of ATP and ADP hydrolysis changes as well as the ATP/ADP ratio in all developmental stages indicated that NTPDase3 was acutely affected after stress. The long-term effect of acute stress on NTPDase activity differed during postnatal development. In juvenile animals (15 days old) NTPDase activity was not altered. However, in later developmental stages (30 and 60 days old rats) NTPDase activity decreased and persisted for 72 h post-stress. In adult rats only ATP hydrolysis was decreased after 24 h, indicating that ecto-ATPase was affected by stress. Ecto-5-nucleotidase hydrolysing activity was decreased within 24 h in adult rats, while in 15- and 30-day old rats it decreased 72 h post-stress. At equivalent times in pubertal rats (60 days old) a slight activation of ecto-5-nucleotidase was detected. Our results highlight the developmental-dependence of brain ecto-nucleotidase susceptibility to acute stress and the likely existence of different mechanisms involved in time-dependent ecto-nucleotidase activity modulation following stress exposure. Clearly there are differences in the response of the purinergic system to acute restraint stress between young and adult rats. (C) 2009 ISDN. Published by Elsevier Ltd. All rights reserved.",
journal = "International Journal of Developmental Neuroscience",
title = "Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development",
volume = "28",
number = "2",
pages = "175-182",
doi = "10.1016/j.ijdevneu.2009.11.005"
}

Horvat, A., Stanojević, I., Drakulić, D. R., Velickovic, N., Petrović, S.,& Milošević, M.. (2010). Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development. in International Journal of Developmental Neuroscience, 28(2), 175-182.
https://doi.org/10.1016/j.ijdevneu.2009.11.005

Horvat A, Stanojević I, Drakulić DR, Velickovic N, Petrović S, Milošević M. Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development. in International Journal of Developmental Neuroscience. 2010;28(2):175-182.
doi:10.1016/j.ijdevneu.2009.11.005 .

Horvat, Anica, Stanojević, Ivana, Drakulić, Dunja R., Velickovic, Natasa, Petrović, Snježana, Milošević, Maja, "Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development" in International Journal of Developmental Neuroscience, 28, no. 2 (2010):175-182,
https://doi.org/10.1016/j.ijdevneu.2009.11.005 . .

TY  - CONF
AU  - Milošević, Maja
AU  - Drakulić, Dunja R.
AU  - Petrović, Snježana
AU  - Stanojević, Ivana
AU  - Veličković, Nataša
AU  - Horvat, Anica
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9319
AB  - L-cysteine is used as effective oral chelating agent due to property of its sulfhydryl
group to bind heavy metal ions. The aim of this study was to investigate ability of
L-cysteine to prevent mercury (II) and cadmium (II) induced ecto-ATPase activity
inhibition of rat uterus plasma membranes. Results show that 10 mmol/l L-cysteine
have protective effect on enzyme activity in the presence of cadmium and mercury
ions.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9319
ER  - 

@conference{
author = "Milošević, Maja and Drakulić, Dunja R. and Petrović, Snježana and Stanojević, Ivana and Veličković, Nataša and Horvat, Anica",
year = "2010",
abstract = "L-cysteine is used as effective oral chelating agent due to property of its sulfhydryl
group to bind heavy metal ions. The aim of this study was to investigate ability of
L-cysteine to prevent mercury (II) and cadmium (II) induced ecto-ATPase activity
inhibition of rat uterus plasma membranes. Results show that 10 mmol/l L-cysteine
have protective effect on enzyme activity in the presence of cadmium and mercury
ions.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9319"
}

Milošević, M., Drakulić, D. R., Petrović, S., Stanojević, I., Veličković, N.,& Horvat, A.. (2010). L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
https://hdl.handle.net/21.15107/rcub_vinar_9319

Milošević M, Drakulić DR, Petrović S, Stanojević I, Veličković N, Horvat A. L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;.
https://hdl.handle.net/21.15107/rcub_vinar_9319 .

Milošević, Maja, Drakulić, Dunja R., Petrović, Snježana, Stanojević, Ivana, Veličković, Nataša, Horvat, Anica, "L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010),
https://hdl.handle.net/21.15107/rcub_vinar_9319 .

TY  - CONF
AU  - Petrović, Snježana
AU  - Milošević, Maja
AU  - Stanojević, Ivana
AU  - Drakulić, Dunja R.
AU  - Jovanović, N.
AU  - Veličković, Nataša
AU  - Horvat, Anica
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9321
AB  - In this study the Ca2+ ion flux modulation in the synaptosomal mitochondria
isolated from caudate nucleus (CN) of the ovariectomised rats was examined.
17 beta-estradiol (E2), E2-conjugated to bovine serum albumin (E-BSA), estradiol
receptor α (ERα) agonist 4,4',4''-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol
(PPT), ERβ agonist 2,3-bis(4-hydroxyphenyl)-propionitrile (DNP) and ERα/β
antagonist 7α,17β-[9[(4,4,5,5,5-pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-
triene-3,17-diol (ICI 182,780) were used. The Ca2+ efflux inhibition of about 27%
was detected in the presence of 0.5 nmol/l E2, and of about 20% in the case of
E-BSA. DNP (10 nmol/l) was as much potent Ca2+ efflux inhibitor as E2, while
PPT (10 nmol/l) hardly had any inhibitory effect (9% efflux decrease). When E2
binding to ERα and ERβ was prevented by 1 μmol/l ICI 182,780, the Ca2+ efflux
inhibition of about 15% was detected. Our results suggest that E2 prevents Ca2+
efflux from synaptosomal mitochondria due to ERβ activation rather than ERα.
The involvement of the external E2 binding site on the mitochondrial membrane
probably different from ERα/β should not be excluded because of Ca2+ efflux
inhibition detected in the presence of E-BSA and ICI 182,780. The Ca2+ efflux
modulation could be the mechanism through which E2 exerts its neuromodulatory
role in specific brain structures.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9321
ER  - 

@conference{
author = "Petrović, Snježana and Milošević, Maja and Stanojević, Ivana and Drakulić, Dunja R. and Jovanović, N. and Veličković, Nataša and Horvat, Anica",
year = "2010",
abstract = "In this study the Ca2+ ion flux modulation in the synaptosomal mitochondria
isolated from caudate nucleus (CN) of the ovariectomised rats was examined.
17 beta-estradiol (E2), E2-conjugated to bovine serum albumin (E-BSA), estradiol
receptor α (ERα) agonist 4,4',4''-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol
(PPT), ERβ agonist 2,3-bis(4-hydroxyphenyl)-propionitrile (DNP) and ERα/β
antagonist 7α,17β-[9[(4,4,5,5,5-pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-
triene-3,17-diol (ICI 182,780) were used. The Ca2+ efflux inhibition of about 27%
was detected in the presence of 0.5 nmol/l E2, and of about 20% in the case of
E-BSA. DNP (10 nmol/l) was as much potent Ca2+ efflux inhibitor as E2, while
PPT (10 nmol/l) hardly had any inhibitory effect (9% efflux decrease). When E2
binding to ERα and ERβ was prevented by 1 μmol/l ICI 182,780, the Ca2+ efflux
inhibition of about 15% was detected. Our results suggest that E2 prevents Ca2+
efflux from synaptosomal mitochondria due to ERβ activation rather than ERα.
The involvement of the external E2 binding site on the mitochondrial membrane
probably different from ERα/β should not be excluded because of Ca2+ efflux
inhibition detected in the presence of E-BSA and ICI 182,780. The Ca2+ efflux
modulation could be the mechanism through which E2 exerts its neuromodulatory
role in specific brain structures.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9321"
}

Petrović, S., Milošević, M., Stanojević, I., Drakulić, D. R., Jovanović, N., Veličković, N.,& Horvat, A.. (2010). Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
https://hdl.handle.net/21.15107/rcub_vinar_9321

Petrović S, Milošević M, Stanojević I, Drakulić DR, Jovanović N, Veličković N, Horvat A. Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;.
https://hdl.handle.net/21.15107/rcub_vinar_9321 .

Petrović, Snježana, Milošević, Maja, Stanojević, Ivana, Drakulić, Dunja R., Jovanović, N., Veličković, Nataša, Horvat, Anica, "Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010),
https://hdl.handle.net/21.15107/rcub_vinar_9321 .

TY  - CONF
AU  - Stanojević, Ivana
AU  - Milošević, Maja
AU  - Petrović, Snježana
AU  - Drakulić, Dunja R.
AU  - Veličković, Nataša
AU  - Horvat, Anica
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9324
AB  - Kinetic properties of ecto-nucleoside triphosphate diphosphohydrolase (NTPDases)
in synaptic terminals from whole rat brain were characterised. Three different
NTPDases are present in nervous system and hydrolysing ATP and ADP with
different affinities. ATP and ADP hydrolysis exhibited kinetic properties typical
for all members of the NTPDase, low substrate specificity for tri- and
diphosphonucleosides, divalent cation dependency and insensitivity towards iontransporting
ATPase inhibitors. Kinetic properties of ATPase and ADPase
component of NTPDases depend on postnatal developmental stages of rats, and
indicated different NTPDase family members present in various ontogeny stages.
Lowering in the NTPDases activities could result in a decreased production of
adenosine and change in extracellular ATP/adenosine ratio.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - Kinetic properties of ntpdases in rat brain synaptic plasma membranes
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9324
ER  - 

@conference{
author = "Stanojević, Ivana and Milošević, Maja and Petrović, Snježana and Drakulić, Dunja R. and Veličković, Nataša and Horvat, Anica",
year = "2010",
abstract = "Kinetic properties of ecto-nucleoside triphosphate diphosphohydrolase (NTPDases)
in synaptic terminals from whole rat brain were characterised. Three different
NTPDases are present in nervous system and hydrolysing ATP and ADP with
different affinities. ATP and ADP hydrolysis exhibited kinetic properties typical
for all members of the NTPDase, low substrate specificity for tri- and
diphosphonucleosides, divalent cation dependency and insensitivity towards iontransporting
ATPase inhibitors. Kinetic properties of ATPase and ADPase
component of NTPDases depend on postnatal developmental stages of rats, and
indicated different NTPDase family members present in various ontogeny stages.
Lowering in the NTPDases activities could result in a decreased production of
adenosine and change in extracellular ATP/adenosine ratio.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "Kinetic properties of ntpdases in rat brain synaptic plasma membranes",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9324"
}

Stanojević, I., Milošević, M., Petrović, S., Drakulić, D. R., Veličković, N.,& Horvat, A.. (2010). Kinetic properties of ntpdases in rat brain synaptic plasma membranes. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
https://hdl.handle.net/21.15107/rcub_vinar_9324

Stanojević I, Milošević M, Petrović S, Drakulić DR, Veličković N, Horvat A. Kinetic properties of ntpdases in rat brain synaptic plasma membranes. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;.
https://hdl.handle.net/21.15107/rcub_vinar_9324 .

Stanojević, Ivana, Milošević, Maja, Petrović, Snježana, Drakulić, Dunja R., Veličković, Nataša, Horvat, Anica, "Kinetic properties of ntpdases in rat brain synaptic plasma membranes" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010),
https://hdl.handle.net/21.15107/rcub_vinar_9324 .