TY  - JOUR
AU  - Vujčić, Miroslava T.
AU  - Terzić, N
AU  - Ristić-Fira, Aleksandra
AU  - Kanazir, Selma
AU  - Ruždijić, Sabera
PY  - 2005
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2906
AB  - In order to contribute to the understanding of mechanisms by which regulatory proteins recognize genetic information stored in DNA, analyses of their interaction with specific nucleotides are usually performed. In this study, the electrophoretic mobility shift assay (EMSA) was applied to analyze the interaction of nuclear proteins from the liver of rats of different age i.e., young (3-month-old), middle-aged (12-month-old) and aged (24-month-old), with radioactively labelled synthetic oligonucleotide analogues, corresponding to GRE. The levels of GRE binding activity were assessed by quantitative densitometric scanning of the autoradiograms. The results showed statistically significant decreasing values of up to 78% and 49% in middle aged and old animals, respectively, compared to young animals (p LT 0.05). The specificity of the nuclear proteins-GRE interaction was demonstrated by competition experiments with unlabelled GRE. In a supershift assay, using the antibody BuGR2, it was shown that the GR proteins present in nuclear extracts have a high affinity for the GRE probe. The stabilities of the protein-DNA complexes were analysed and it was concluded that they changed during ageing.
T2  - Journal of the Serbian Chemical Society
T1  - Analysis of nuclear glucocorticoid receptor-DNA interaction in aged rat liver
VL  - 70
IS  - 5
SP  - 705
EP  - 712
DO  - 10.2298/JSC0505705V
ER  - 

@article{
author = "Vujčić, Miroslava T. and Terzić, N and Ristić-Fira, Aleksandra and Kanazir, Selma and Ruždijić, Sabera",
year = "2005",
abstract = "In order to contribute to the understanding of mechanisms by which regulatory proteins recognize genetic information stored in DNA, analyses of their interaction with specific nucleotides are usually performed. In this study, the electrophoretic mobility shift assay (EMSA) was applied to analyze the interaction of nuclear proteins from the liver of rats of different age i.e., young (3-month-old), middle-aged (12-month-old) and aged (24-month-old), with radioactively labelled synthetic oligonucleotide analogues, corresponding to GRE. The levels of GRE binding activity were assessed by quantitative densitometric scanning of the autoradiograms. The results showed statistically significant decreasing values of up to 78% and 49% in middle aged and old animals, respectively, compared to young animals (p LT 0.05). The specificity of the nuclear proteins-GRE interaction was demonstrated by competition experiments with unlabelled GRE. In a supershift assay, using the antibody BuGR2, it was shown that the GR proteins present in nuclear extracts have a high affinity for the GRE probe. The stabilities of the protein-DNA complexes were analysed and it was concluded that they changed during ageing.",
journal = "Journal of the Serbian Chemical Society",
title = "Analysis of nuclear glucocorticoid receptor-DNA interaction in aged rat liver",
volume = "70",
number = "5",
pages = "705-712",
doi = "10.2298/JSC0505705V"
}

Vujčić, M. T., Terzić, N., Ristić-Fira, A., Kanazir, S.,& Ruždijić, S.. (2005). Analysis of nuclear glucocorticoid receptor-DNA interaction in aged rat liver. in Journal of the Serbian Chemical Society, 70(5), 705-712.
https://doi.org/10.2298/JSC0505705V

Vujčić MT, Terzić N, Ristić-Fira A, Kanazir S, Ruždijić S. Analysis of nuclear glucocorticoid receptor-DNA interaction in aged rat liver. in Journal of the Serbian Chemical Society. 2005;70(5):705-712.
doi:10.2298/JSC0505705V .

Vujčić, Miroslava T., Terzić, N, Ristić-Fira, Aleksandra, Kanazir, Selma, Ruždijić, Sabera, "Analysis of nuclear glucocorticoid receptor-DNA interaction in aged rat liver" in Journal of the Serbian Chemical Society, 70, no. 5 (2005):705-712,
https://doi.org/10.2298/JSC0505705V . .

TY  - JOUR
AU  - Terzić, N
AU  - Vujčić, Miroslava T.
AU  - Ristić-Fira, Aleksandra
AU  - Krstić-Demonacos, Marija
AU  - Milanovic, D
AU  - Kanazir, Dušan T.
AU  - Ruždijić, Sabera
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2623
AB  - The effect of dexamethasone (DEX) on glucocorticoid receptor (GR)-mediated gene expression was examined in the brain of young and aged rats. Electrophoretic mobility shift assays showed that DEX treatment led to an increase of glucocorticoid response element (GRE) binding activity in aged rats, whereas in young animals GRE binding activity was decreased. Western blot analysis and reverse transcriptase polymerase chain reaction confirmed that, in aged animals, the GR mRNA and the GR protein levels were increased on DEX treatment. The binding activity of GRE activating protein-1 (AP-1) site and cross-competition analysis demonstrated specific pattern of expression during the ageing and DEX treatment, suggesting that GR modulates the activity of transcription factors AP-1 (Fos/Jun proteins) through protein-protein interaction. On the basis of these results, it can be concluded that the composition of transcriptional complexes that bind to GRE and AP-I regulatory elements changes upon DEX treatment in an age-specific manner.
T2  - Journals of Gerontology. Series A: Biological Sciences and Medical Sciences
T1  - Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain
VL  - 58
IS  - 4
SP  - 297
EP  - 303
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2623
ER  - 

@article{
author = "Terzić, N and Vujčić, Miroslava T. and Ristić-Fira, Aleksandra and Krstić-Demonacos, Marija and Milanovic, D and Kanazir, Dušan T. and Ruždijić, Sabera",
year = "2003",
abstract = "The effect of dexamethasone (DEX) on glucocorticoid receptor (GR)-mediated gene expression was examined in the brain of young and aged rats. Electrophoretic mobility shift assays showed that DEX treatment led to an increase of glucocorticoid response element (GRE) binding activity in aged rats, whereas in young animals GRE binding activity was decreased. Western blot analysis and reverse transcriptase polymerase chain reaction confirmed that, in aged animals, the GR mRNA and the GR protein levels were increased on DEX treatment. The binding activity of GRE activating protein-1 (AP-1) site and cross-competition analysis demonstrated specific pattern of expression during the ageing and DEX treatment, suggesting that GR modulates the activity of transcription factors AP-1 (Fos/Jun proteins) through protein-protein interaction. On the basis of these results, it can be concluded that the composition of transcriptional complexes that bind to GRE and AP-I regulatory elements changes upon DEX treatment in an age-specific manner.",
journal = "Journals of Gerontology. Series A: Biological Sciences and Medical Sciences",
title = "Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain",
volume = "58",
number = "4",
pages = "297-303",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2623"
}

Terzić, N., Vujčić, M. T., Ristić-Fira, A., Krstić-Demonacos, M., Milanovic, D., Kanazir, D. T.,& Ruždijić, S.. (2003). Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain. in Journals of Gerontology. Series A: Biological Sciences and Medical Sciences, 58(4), 297-303.
https://hdl.handle.net/21.15107/rcub_vinar_2623

Terzić N, Vujčić MT, Ristić-Fira A, Krstić-Demonacos M, Milanovic D, Kanazir DT, Ruždijić S. Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain. in Journals of Gerontology. Series A: Biological Sciences and Medical Sciences. 2003;58(4):297-303.
https://hdl.handle.net/21.15107/rcub_vinar_2623 .

Terzić, N, Vujčić, Miroslava T., Ristić-Fira, Aleksandra, Krstić-Demonacos, Marija, Milanovic, D, Kanazir, Dušan T., Ruždijić, Sabera, "Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain" in Journals of Gerontology. Series A: Biological Sciences and Medical Sciences, 58, no. 4 (2003):297-303,
https://hdl.handle.net/21.15107/rcub_vinar_2623 .