TY  - CONF
AU  - Masnikosa, Romana
AU  - Pirić, David
AU  - Cvetković, Zorica
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12413
AB  - Both cancer and inflammation are almost invariably accompanied by lipidome dysregulation. Hence, various lipid species have been reported as candidate markers for many solid tumours1-3. However, neither the global lipidome nor sub-lipidome of inflammatory pathways in Non-Hodgkin lymphoma (NHL) has been studied. In order to fill this gap and shed light on the inflammatory pathways accompanying NHL, we designed a targeted liquid chromatography – Multiple Reaction Monitoring of bioactive lipids/lipid mediators in plasma of female patients with Diffuse Large B-cell Lymphoma (DLBCL), the most often type of NHL. We chose to quantify lipids known or hypothesized to be involved in inflammation and cancer progression along with their membrane precursors. In a pilot study encompassing plasma samples from 17 DLBCL patients and 21 BMI-matched controls, we analysed levels of pro-inlammatory arachidonic acid (AA)-derived oxylipins, focusing on lipoxygenase (LOX) and cytochrome P450 monooxygenase products: hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids; several AA-containing phospholipids (PLs); specificlysophospholipid subclasses; sphingomyelins (SMs), sphingosine 1-phosphate (S1P) and polyunsaturated fatty acids. Data were subjected to classical statistics and multivariate unsupervised and supervised machine learning (ML) algorithms. The DLBCL status was profoundly associated with altered S1P, SM 34:1, SM 36:1 and phosphatidylinositol PI 34:1 abundance. On the other hand, eicosanoids 12(S)-HETE, 15(S)-HETE and thromboxane B2 were major lipid species dis criminating between DLBCL and healthy status, as well as lysophosphatidylinositol LPI 20:4. The correlations between lipid species varied considerably between the cancer and controls, reflecting significant changes in lipid metabolic and/or signalling pathways, particularly those within LOX pathway and cell membrane PL remodelling. We suggest S1P, SM 36:1, SM 34:1 and PI 34:1 may beviewed as lipid signatures of DLBCL. Furthermore, these four lipid species could serve as a basis for the prospective validation in larger DLBCL/NHL clinical studies. As far as we know, this is the first plasma lipid profiling in DLBCL/NHL and, as such, brings new knowledge on the metabolic basis of inflammation in this cancer. The added value of our plasma lipid profiling in DLBCL is a deeper understanding of particulate lipid dysregulations in this tumour.
PB  - Kragujevac : Faculty of Science, University of Kragujevac
C3  - SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts
T1  - Plasma Profile of Inflamatory Mediators in NHL Patients
SP  - OP5
EP  - OP5
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12413
ER  - 

@conference{
author = "Masnikosa, Romana and Pirić, David and Cvetković, Zorica",
year = "2023",
abstract = "Both cancer and inflammation are almost invariably accompanied by lipidome dysregulation. Hence, various lipid species have been reported as candidate markers for many solid tumours1-3. However, neither the global lipidome nor sub-lipidome of inflammatory pathways in Non-Hodgkin lymphoma (NHL) has been studied. In order to fill this gap and shed light on the inflammatory pathways accompanying NHL, we designed a targeted liquid chromatography – Multiple Reaction Monitoring of bioactive lipids/lipid mediators in plasma of female patients with Diffuse Large B-cell Lymphoma (DLBCL), the most often type of NHL. We chose to quantify lipids known or hypothesized to be involved in inflammation and cancer progression along with their membrane precursors. In a pilot study encompassing plasma samples from 17 DLBCL patients and 21 BMI-matched controls, we analysed levels of pro-inlammatory arachidonic acid (AA)-derived oxylipins, focusing on lipoxygenase (LOX) and cytochrome P450 monooxygenase products: hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids; several AA-containing phospholipids (PLs); specificlysophospholipid subclasses; sphingomyelins (SMs), sphingosine 1-phosphate (S1P) and polyunsaturated fatty acids. Data were subjected to classical statistics and multivariate unsupervised and supervised machine learning (ML) algorithms. The DLBCL status was profoundly associated with altered S1P, SM 34:1, SM 36:1 and phosphatidylinositol PI 34:1 abundance. On the other hand, eicosanoids 12(S)-HETE, 15(S)-HETE and thromboxane B2 were major lipid species dis criminating between DLBCL and healthy status, as well as lysophosphatidylinositol LPI 20:4. The correlations between lipid species varied considerably between the cancer and controls, reflecting significant changes in lipid metabolic and/or signalling pathways, particularly those within LOX pathway and cell membrane PL remodelling. We suggest S1P, SM 36:1, SM 34:1 and PI 34:1 may beviewed as lipid signatures of DLBCL. Furthermore, these four lipid species could serve as a basis for the prospective validation in larger DLBCL/NHL clinical studies. As far as we know, this is the first plasma lipid profiling in DLBCL/NHL and, as such, brings new knowledge on the metabolic basis of inflammation in this cancer. The added value of our plasma lipid profiling in DLBCL is a deeper understanding of particulate lipid dysregulations in this tumour.",
publisher = "Kragujevac : Faculty of Science, University of Kragujevac",
journal = "SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts",
title = "Plasma Profile of Inflamatory Mediators in NHL Patients",
pages = "OP5-OP5",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12413"
}

Masnikosa, R., Pirić, D.,& Cvetković, Z.. (2023). Plasma Profile of Inflamatory Mediators in NHL Patients. in SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts
Kragujevac : Faculty of Science, University of Kragujevac., OP5-OP5.
https://hdl.handle.net/21.15107/rcub_vinar_12413

Masnikosa R, Pirić D, Cvetković Z. Plasma Profile of Inflamatory Mediators in NHL Patients. in SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts. 2023;:OP5-OP5.
https://hdl.handle.net/21.15107/rcub_vinar_12413 .

Masnikosa, Romana, Pirić, David, Cvetković, Zorica, "Plasma Profile of Inflamatory Mediators in NHL Patients" in SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts (2023):OP5-OP5,
https://hdl.handle.net/21.15107/rcub_vinar_12413 .

TY  - CONF
AU  - Masnikosa, Romana
AU  - Pirić, David
AU  - Cvetković, Zorica
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12257
AB  - Both cancer and inflammation are almost invariably accompanied by lipidome dysregulation. Hence, various lipid species have been reported as candidate markers for many solid tumours 1-3 .However, neitherthe globallipidome norsub-lipidome ofinflammatory pathways in Non-Hodgkin lymphoma (NHL) has been studied. In order to fill this gap and shed light on the inflammatory pathways accompanying NHL, we designed a targeted liquid chromatography – Multiple Reaction Monitoring of bioactive lipids/lipid mediators in plasma of female patients with Diffuse Large B-cell Lymphoma (DLBCL), the most often type of NHL. We chose to quantify lipids known or hypothesized to be involved in inflammation and cancer progression along with theirmembrane precursors. In a pilot study encompassing plasma samples from 17 DLBCL patients and 21 BMI-matched controls, we analysed levels of pro-inlammatory arachidonic acid (AA)-derived oxylipins, focusing on lipoxygenase (LOX) and cytochrome P450 monooxygenase products: hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids; several AA-containing phospholipids (PLs); specificlysophospholipid subclasses; sphingomyelins (SMs), sphingosine 1-phosphate (S1P) and polyunsaturated fatty acids. Data were subjected to classical statistics and multivariate unsupervised and supervised machine learning (ML) algorithms. The DLBCL status was profoundly associated with altered S1P, SM 34:1, SM 36:1 and phosphatidylinositol PI 34:1 abundance. On the other hand, eicosanoids 12(S)-HETE, 15(S)-HETE and thromboxane B2 were major lipid species discriminating between DLBCL and healthy status, as well as lysophosphatidylinositol LPI 20:4. The correlations between lipid species varied considerably between the cancer and controls, reflecting significant changes in lipid metabolic and/or signalling pathways, particularly those within LOX pathway and cell membrane PL remodelling. We suggest S1P, SM 36:1, SM 34:1 and PI 34:1 may beviewed as lipid signatures of DLBCL. Furthermore, these four lipid species could serve asa basis for the prospective validation in larger DLBCL/NHL clinical studies. As far as we know, this is the first plasma lipid profiling in DLBCL/NHL and, as such, brings new knowledge on the metabolic basis of inflammation in this cancer. The added value of our plasma lipid profiling in DLBCL is a deeper understanding of particulate lipid dysregulations in this tumour
PB  - Belgrade : Faculty of Science, University of Kragujevac
C3  - SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts
T1  - Plasma Profile of Inflamatory Mediators in NHL Patients
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12257
ER  - 

@conference{
author = "Masnikosa, Romana and Pirić, David and Cvetković, Zorica",
year = "2023",
abstract = "Both cancer and inflammation are almost invariably accompanied by lipidome dysregulation. Hence, various lipid species have been reported as candidate markers for many solid tumours 1-3 .However, neitherthe globallipidome norsub-lipidome ofinflammatory pathways in Non-Hodgkin lymphoma (NHL) has been studied. In order to fill this gap and shed light on the inflammatory pathways accompanying NHL, we designed a targeted liquid chromatography – Multiple Reaction Monitoring of bioactive lipids/lipid mediators in plasma of female patients with Diffuse Large B-cell Lymphoma (DLBCL), the most often type of NHL. We chose to quantify lipids known or hypothesized to be involved in inflammation and cancer progression along with theirmembrane precursors. In a pilot study encompassing plasma samples from 17 DLBCL patients and 21 BMI-matched controls, we analysed levels of pro-inlammatory arachidonic acid (AA)-derived oxylipins, focusing on lipoxygenase (LOX) and cytochrome P450 monooxygenase products: hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids; several AA-containing phospholipids (PLs); specificlysophospholipid subclasses; sphingomyelins (SMs), sphingosine 1-phosphate (S1P) and polyunsaturated fatty acids. Data were subjected to classical statistics and multivariate unsupervised and supervised machine learning (ML) algorithms. The DLBCL status was profoundly associated with altered S1P, SM 34:1, SM 36:1 and phosphatidylinositol PI 34:1 abundance. On the other hand, eicosanoids 12(S)-HETE, 15(S)-HETE and thromboxane B2 were major lipid species discriminating between DLBCL and healthy status, as well as lysophosphatidylinositol LPI 20:4. The correlations between lipid species varied considerably between the cancer and controls, reflecting significant changes in lipid metabolic and/or signalling pathways, particularly those within LOX pathway and cell membrane PL remodelling. We suggest S1P, SM 36:1, SM 34:1 and PI 34:1 may beviewed as lipid signatures of DLBCL. Furthermore, these four lipid species could serve asa basis for the prospective validation in larger DLBCL/NHL clinical studies. As far as we know, this is the first plasma lipid profiling in DLBCL/NHL and, as such, brings new knowledge on the metabolic basis of inflammation in this cancer. The added value of our plasma lipid profiling in DLBCL is a deeper understanding of particulate lipid dysregulations in this tumour",
publisher = "Belgrade : Faculty of Science, University of Kragujevac",
journal = "SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts",
title = "Plasma Profile of Inflamatory Mediators in NHL Patients",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12257"
}

Masnikosa, R., Pirić, D.,& Cvetković, Z.. (2023). Plasma Profile of Inflamatory Mediators in NHL Patients. in SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts
Belgrade : Faculty of Science, University of Kragujevac..
https://hdl.handle.net/21.15107/rcub_vinar_12257

Masnikosa R, Pirić D, Cvetković Z. Plasma Profile of Inflamatory Mediators in NHL Patients. in SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts. 2023;.
https://hdl.handle.net/21.15107/rcub_vinar_12257 .

Masnikosa, Romana, Pirić, David, Cvetković, Zorica, "Plasma Profile of Inflamatory Mediators in NHL Patients" in SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts (2023),
https://hdl.handle.net/21.15107/rcub_vinar_12257 .

TY  - JOUR
AU  - Masnikosa, Romana
AU  - Pirić, David
AU  - Post, Julia Maria
AU  - Cvetković, Zorica
AU  - Petrović, Snježana
AU  - Paunović, Marija
AU  - Vučić, Vesna
AU  - Bindila, Laura
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11437
AB  - Lipidome dysregulation is a hallmark of cancer and inflammation. The global plasma lipidome and sub-lipidome of inflammatory pathways have not been reported in diffuse large B-cell lymphoma (DLBCL). In a pilot study of plasma lipid variation in female DLBCL patients and BMI-matched disease-free controls, we performed targeted lipidomics using LC-MRM to quantify lipid mediators of inflammation and immunity, and those known or hypothesised to be involved in cancer progression: sphingolipids, resolvin D1, arachidonic acid (AA)-derived oxylipins, such as hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids, along with their membrane structural precursors. We report on the role of the eicosanoids in the separation of DLBCL from controls, along with lysophosphatidylinositol LPI 20:4, implying notable changes in lipid metabolic and/or signalling pathways, particularly pertaining to AA lipoxygenase pathway and glycerophospholipid remodelling in the cell membrane. We suggest here the set of S1P, SM 36:1, SM 34:1 and PI 34:1 as DLBCL lipid signatures which could serve as a basis for the prospective validation in larger DLBCL cohorts. Additionally, untargeted lipidomics indicates a substantial change in the overall lipid metabolism in DLBCL. The plasma lipid profiling of DLBCL patients helps to better understand the specific lipid dysregulations and pathways in this cancer.
T2  - Cancers
T1  - Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study
VL  - 15
IS  - 14
SP  - 3653
DO  - 10.3390/cancers15143653
ER  - 

@article{
author = "Masnikosa, Romana and Pirić, David and Post, Julia Maria and Cvetković, Zorica and Petrović, Snježana and Paunović, Marija and Vučić, Vesna and Bindila, Laura",
year = "2023",
abstract = "Lipidome dysregulation is a hallmark of cancer and inflammation. The global plasma lipidome and sub-lipidome of inflammatory pathways have not been reported in diffuse large B-cell lymphoma (DLBCL). In a pilot study of plasma lipid variation in female DLBCL patients and BMI-matched disease-free controls, we performed targeted lipidomics using LC-MRM to quantify lipid mediators of inflammation and immunity, and those known or hypothesised to be involved in cancer progression: sphingolipids, resolvin D1, arachidonic acid (AA)-derived oxylipins, such as hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids, along with their membrane structural precursors. We report on the role of the eicosanoids in the separation of DLBCL from controls, along with lysophosphatidylinositol LPI 20:4, implying notable changes in lipid metabolic and/or signalling pathways, particularly pertaining to AA lipoxygenase pathway and glycerophospholipid remodelling in the cell membrane. We suggest here the set of S1P, SM 36:1, SM 34:1 and PI 34:1 as DLBCL lipid signatures which could serve as a basis for the prospective validation in larger DLBCL cohorts. Additionally, untargeted lipidomics indicates a substantial change in the overall lipid metabolism in DLBCL. The plasma lipid profiling of DLBCL patients helps to better understand the specific lipid dysregulations and pathways in this cancer.",
journal = "Cancers",
title = "Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study",
volume = "15",
number = "14",
pages = "3653",
doi = "10.3390/cancers15143653"
}

Masnikosa, R., Pirić, D., Post, J. M., Cvetković, Z., Petrović, S., Paunović, M., Vučić, V.,& Bindila, L.. (2023). Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study. in Cancers, 15(14), 3653.
https://doi.org/10.3390/cancers15143653

Masnikosa R, Pirić D, Post JM, Cvetković Z, Petrović S, Paunović M, Vučić V, Bindila L. Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study. in Cancers. 2023;15(14):3653.
doi:10.3390/cancers15143653 .

Masnikosa, Romana, Pirić, David, Post, Julia Maria, Cvetković, Zorica, Petrović, Snježana, Paunović, Marija, Vučić, Vesna, Bindila, Laura, "Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study" in Cancers, 15, no. 14 (2023):3653,
https://doi.org/10.3390/cancers15143653 . .

TY  - JOUR
AU  - Kolesnikova, I. A.
AU  - Lalkovičova, M.
AU  - Severyukhin, Yu. S.
AU  - Golikova, K. N.
AU  - Utina, D. M.
AU  - Pronskikh, E. V.
AU  - Despotović, Sanja Z.
AU  - Gaevsky, V. N.
AU  - Pirić, David
AU  - Masnikosa, Romana
AU  - Budennaya, N. N.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11224
AB  - We designed a study with the objective to determine the long-term radiation effects of gamma rays, originating from a single shot of Co60 at a dose of 2 Gy on the 7-month-old male mice of the ICR line in 30 days after the irradiation. The aim of this study was to characterize the behavior of animals using the Open Field test, immuno-hematological status, and morpho-functional changes in the central nervous system of mice. Irradiated animals displayed significantly different behavior in the OF in comparison with the control group. The radiation damage was confirmed by assessing the ratio of leukocytes in the peripheral blood of mice at a later date after exposure to Co60. After irradiation, a decrease in the glioneuronal complex was observed in the irritated group as well as histological changes of brain cells. To sum up, not only was the hematological status of mice altered upon the total gamma irradiation, but also their behavior, which was most probably due to significant alterations in the CNS. Graphical Abstract: Study of influence of ionizing radiation on female mice, comparison between different age groups. Open Field test on the 30 days after 2 Gy of γ-rays and histological analysis indicated changes in behavioral patterns, leucocytes, and brain tissue. [Figure not available: see fulltext.] © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Cellular and Molecular Neurobiology
T1  - The Effects of Whole Body Gamma Irradiation on Mice, Age-Related Behavioral, and Pathophysiological Changes
VL  - InPress
DO  - 10.1007/s10571-023-01381-1
ER  - 

@article{
author = "Kolesnikova, I. A. and Lalkovičova, M. and Severyukhin, Yu. S. and Golikova, K. N. and Utina, D. M. and Pronskikh, E. V. and Despotović, Sanja Z. and Gaevsky, V. N. and Pirić, David and Masnikosa, Romana and Budennaya, N. N.",
year = "2023",
abstract = "We designed a study with the objective to determine the long-term radiation effects of gamma rays, originating from a single shot of Co60 at a dose of 2 Gy on the 7-month-old male mice of the ICR line in 30 days after the irradiation. The aim of this study was to characterize the behavior of animals using the Open Field test, immuno-hematological status, and morpho-functional changes in the central nervous system of mice. Irradiated animals displayed significantly different behavior in the OF in comparison with the control group. The radiation damage was confirmed by assessing the ratio of leukocytes in the peripheral blood of mice at a later date after exposure to Co60. After irradiation, a decrease in the glioneuronal complex was observed in the irritated group as well as histological changes of brain cells. To sum up, not only was the hematological status of mice altered upon the total gamma irradiation, but also their behavior, which was most probably due to significant alterations in the CNS. Graphical Abstract: Study of influence of ionizing radiation on female mice, comparison between different age groups. Open Field test on the 30 days after 2 Gy of γ-rays and histological analysis indicated changes in behavioral patterns, leucocytes, and brain tissue. [Figure not available: see fulltext.] © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Cellular and Molecular Neurobiology",
title = "The Effects of Whole Body Gamma Irradiation on Mice, Age-Related Behavioral, and Pathophysiological Changes",
volume = "InPress",
doi = "10.1007/s10571-023-01381-1"
}

Kolesnikova, I. A., Lalkovičova, M., Severyukhin, Yu. S., Golikova, K. N., Utina, D. M., Pronskikh, E. V., Despotović, S. Z., Gaevsky, V. N., Pirić, D., Masnikosa, R.,& Budennaya, N. N.. (2023). The Effects of Whole Body Gamma Irradiation on Mice, Age-Related Behavioral, and Pathophysiological Changes. in Cellular and Molecular Neurobiology, InPress.
https://doi.org/10.1007/s10571-023-01381-1

Kolesnikova IA, Lalkovičova M, Severyukhin YS, Golikova KN, Utina DM, Pronskikh EV, Despotović SZ, Gaevsky VN, Pirić D, Masnikosa R, Budennaya NN. The Effects of Whole Body Gamma Irradiation on Mice, Age-Related Behavioral, and Pathophysiological Changes. in Cellular and Molecular Neurobiology. 2023;InPress.
doi:10.1007/s10571-023-01381-1 .

Kolesnikova, I. A., Lalkovičova, M., Severyukhin, Yu. S., Golikova, K. N., Utina, D. M., Pronskikh, E. V., Despotović, Sanja Z., Gaevsky, V. N., Pirić, David, Masnikosa, Romana, Budennaya, N. N., "The Effects of Whole Body Gamma Irradiation on Mice, Age-Related Behavioral, and Pathophysiological Changes" in Cellular and Molecular Neurobiology, InPress (2023),
https://doi.org/10.1007/s10571-023-01381-1 . .