TY  - CONF
AU  - Joksić, Gordana
AU  - Guč-Šćekić, Marija
AU  - Vujić, Dragana
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Jevtić, D.
AU  - Mićić, D.
PY  - 2007
PY  - 1
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11735
AB  - Fanconi anemia (FA) is an autosomal recessive disorder characterized by bone-marrow failure and cancer susceptibility. Early studies pointed to the specific sensitivity of FA cells to MMC and DEB which became the tools for the current diagnostic tests for FA. Although there is the long-standing clinical impression of radiosensitivity, in vitro studies have yielded conflicting results. We exposed peripheral blood mononuclear cells of FA patients (10 subjects) and carriers (20 subjects) to γ-rays (60Co), dose of 2 Gy in vitro with aim to determine their radiosensitivity using CB micronucleus (MN) test. Incidence of spontaneous occurring chromosomal aberrations and MN in unirradiatedcontrol samples also was examined. Mean incidence of chromosomal aberrations in FA patients was 0.088±0.08; which is 3.5 fold higher than in carriers, baseline level of MN was 11.66±6.7 whereas average incidence of radiation-induced micronuclei was 122.63±95.6. Baseline level of micronuclei find in parents lymphocytes was 16.29±8.4 (fathers); 14.04±11.17 (mothers), which is 2.3 fold higher compared to common population. Radioresponse of FA lymphocyte in vitro in most cases corresponds to resistant in vitro response, (with exception of one case where radiosensitive in vitro response was observed). Incidence of spontaneously occurring chromosomal aberration highly correlates with baseline incidence of MN, number of cell carrying aberrations and radiosensitivity (r=0.81, p<0.05) Mild radioresistant in vitro response was observed in mothers lymphocytes, whereas fathers response could be described normal-as in the common population. The authors will discuss the possible explanations for resistant response of FA lymphocytes in vitro
PB  - Nature Publishing Group
C3  - Proceedings of the European Journal of Human Genetics
T1  - Radiosensitivity of Fanconi anemia lymphocytes in vitro measured by CBMN assay
VL  - 15
SP  - 108
EP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11735
ER  - 

@conference{
author = "Joksić, Gordana and Guč-Šćekić, Marija and Vujić, Dragana and Petrović, Sandra and Leskovac, Andreja and Jevtić, D. and Mićić, D.",
year = "2007, 1",
abstract = "Fanconi anemia (FA) is an autosomal recessive disorder characterized by bone-marrow failure and cancer susceptibility. Early studies pointed to the specific sensitivity of FA cells to MMC and DEB which became the tools for the current diagnostic tests for FA. Although there is the long-standing clinical impression of radiosensitivity, in vitro studies have yielded conflicting results. We exposed peripheral blood mononuclear cells of FA patients (10 subjects) and carriers (20 subjects) to γ-rays (60Co), dose of 2 Gy in vitro with aim to determine their radiosensitivity using CB micronucleus (MN) test. Incidence of spontaneous occurring chromosomal aberrations and MN in unirradiatedcontrol samples also was examined. Mean incidence of chromosomal aberrations in FA patients was 0.088±0.08; which is 3.5 fold higher than in carriers, baseline level of MN was 11.66±6.7 whereas average incidence of radiation-induced micronuclei was 122.63±95.6. Baseline level of micronuclei find in parents lymphocytes was 16.29±8.4 (fathers); 14.04±11.17 (mothers), which is 2.3 fold higher compared to common population. Radioresponse of FA lymphocyte in vitro in most cases corresponds to resistant in vitro response, (with exception of one case where radiosensitive in vitro response was observed). Incidence of spontaneously occurring chromosomal aberration highly correlates with baseline incidence of MN, number of cell carrying aberrations and radiosensitivity (r=0.81, p<0.05) Mild radioresistant in vitro response was observed in mothers lymphocytes, whereas fathers response could be described normal-as in the common population. The authors will discuss the possible explanations for resistant response of FA lymphocytes in vitro",
publisher = "Nature Publishing Group",
journal = "Proceedings of the European Journal of Human Genetics",
title = "Radiosensitivity of Fanconi anemia lymphocytes in vitro measured by CBMN assay",
volume = "15",
pages = "108-109",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11735"
}

Joksić, G., Guč-Šćekić, M., Vujić, D., Petrović, S., Leskovac, A., Jevtić, D.,& Mićić, D.. (2007). Radiosensitivity of Fanconi anemia lymphocytes in vitro measured by CBMN assay. in Proceedings of the European Journal of Human Genetics
Nature Publishing Group., 15, 108-109.
https://hdl.handle.net/21.15107/rcub_vinar_11735

Joksić G, Guč-Šćekić M, Vujić D, Petrović S, Leskovac A, Jevtić D, Mićić D. Radiosensitivity of Fanconi anemia lymphocytes in vitro measured by CBMN assay. in Proceedings of the European Journal of Human Genetics. 2007;15:108-109.
https://hdl.handle.net/21.15107/rcub_vinar_11735 .

Joksić, Gordana, Guč-Šćekić, Marija, Vujić, Dragana, Petrović, Sandra, Leskovac, Andreja, Jevtić, D., Mićić, D., "Radiosensitivity of Fanconi anemia lymphocytes in vitro measured by CBMN assay" in Proceedings of the European Journal of Human Genetics, 15 (2007):108-109,
https://hdl.handle.net/21.15107/rcub_vinar_11735 .