TY  - JOUR
AU  - Kostić, Milan
AU  - Korićanac, Goran
AU  - Tepavčević, Snežana
AU  - Stanišić, Jelena
AU  - Romić, Snježana
AU  - Ćulafić, Tijana
AU  - Ivković, Tamara
AU  - Stojiljković, Mojca
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13109
AB  - Context: Excessive fructose consumption causes ectopic lipid storage leading to metabolic disorders and cardiovascular diseases associated with defective substrate utilisation in the heart. Objective: Examining the preventive impact of low-intensity exercise on alterations related to fructose-rich diet (FRD) on cardiac fatty acid (FA) transport and metabolism. Materials and methods: Male Wistar rats were divided into control and two groups that received 10% fructose for 9 weeks, one of which was additionally exposed to exercise. Results: FRD elevated plasma and cardiac TAG, FATP1 in plasma membrane, Lipin 1 in microsomes and HSL mRNA, while mitochondrial CPT1 was decreased. Exercise decreased plasma free FA level, raised CD36 in plasma membrane and FATP1 in lysate, mitochondrial CPT1 and decreased microsomal Lipin 1 in fructose-fed rats. Conclusions: FRD changed plasma lipids and augmented partitioning of FA to TAG storage in the heart, whereas exercise in FRD rats switched metabolism of FA towards β-oxidation.
T2  - Archives of Physiology and Biochemistry
T1  - Low-intensity exercise diverts cardiac fatty acid metabolism from triacylglycerol synthesis to beta oxidation in fructose-fed rats
VL  - 129
IS  - 4
SP  - 922
EP  - 932
DO  - 10.1080/13813455.2021.1886118
ER  - 

@article{
author = "Kostić, Milan and Korićanac, Goran and Tepavčević, Snežana and Stanišić, Jelena and Romić, Snježana and Ćulafić, Tijana and Ivković, Tamara and Stojiljković, Mojca",
year = "2023",
abstract = "Context: Excessive fructose consumption causes ectopic lipid storage leading to metabolic disorders and cardiovascular diseases associated with defective substrate utilisation in the heart. Objective: Examining the preventive impact of low-intensity exercise on alterations related to fructose-rich diet (FRD) on cardiac fatty acid (FA) transport and metabolism. Materials and methods: Male Wistar rats were divided into control and two groups that received 10% fructose for 9 weeks, one of which was additionally exposed to exercise. Results: FRD elevated plasma and cardiac TAG, FATP1 in plasma membrane, Lipin 1 in microsomes and HSL mRNA, while mitochondrial CPT1 was decreased. Exercise decreased plasma free FA level, raised CD36 in plasma membrane and FATP1 in lysate, mitochondrial CPT1 and decreased microsomal Lipin 1 in fructose-fed rats. Conclusions: FRD changed plasma lipids and augmented partitioning of FA to TAG storage in the heart, whereas exercise in FRD rats switched metabolism of FA towards β-oxidation.",
journal = "Archives of Physiology and Biochemistry",
title = "Low-intensity exercise diverts cardiac fatty acid metabolism from triacylglycerol synthesis to beta oxidation in fructose-fed rats",
volume = "129",
number = "4",
pages = "922-932",
doi = "10.1080/13813455.2021.1886118"
}

Kostić, M., Korićanac, G., Tepavčević, S., Stanišić, J., Romić, S., Ćulafić, T., Ivković, T.,& Stojiljković, M.. (2023). Low-intensity exercise diverts cardiac fatty acid metabolism from triacylglycerol synthesis to beta oxidation in fructose-fed rats. in Archives of Physiology and Biochemistry, 129(4), 922-932.
https://doi.org/10.1080/13813455.2021.1886118

Kostić M, Korićanac G, Tepavčević S, Stanišić J, Romić S, Ćulafić T, Ivković T, Stojiljković M. Low-intensity exercise diverts cardiac fatty acid metabolism from triacylglycerol synthesis to beta oxidation in fructose-fed rats. in Archives of Physiology and Biochemistry. 2023;129(4):922-932.
doi:10.1080/13813455.2021.1886118 .

Kostić, Milan, Korićanac, Goran, Tepavčević, Snežana, Stanišić, Jelena, Romić, Snježana, Ćulafić, Tijana, Ivković, Tamara, Stojiljković, Mojca, "Low-intensity exercise diverts cardiac fatty acid metabolism from triacylglycerol synthesis to beta oxidation in fructose-fed rats" in Archives of Physiology and Biochemistry, 129, no. 4 (2023):922-932,
https://doi.org/10.1080/13813455.2021.1886118 . .

TY  - JOUR
AU  - Kostić, Milan
AU  - Korićanac, Goran
AU  - Tepavčević, Snežana
AU  - Stanišić, Jelena
AU  - Romić, Snježana Đ.
AU  - Ćulafić, Tijana
AU  - Ivković, Tamara
AU  - Stojiljković, Mojca D.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10747
AB  - Background and Aim: Excessive fructose consumption along with a sedentary lifestyle provokes metabolic disorders and cardiovascular diseases. Fructose overload causes cardiac insulin resistance and increases reliance on fatty acid (FA) uptake and catabolism. The cardiometabolic benefits of exercise training have long been appreciated. The goal of the presented study is to shed a new light to the preventive role of exercise training on cardiac lipid metabolism in fructose-fed rats. Methods: Male Wistar rats were divided into control (C), sedentary fructose (F), and exercised fructose (EF) groups. Fructose was given as a 10% fructose solution in drinking water for 9 weeks. Low-intensity exercise training was applied for 9 weeks. The protein expression and subcellular localization of Lipin1, peroxisome proliferator-activated receptor α (PPARα), and peroxisome proliferator-activated receptor-γcoactivator 1 α (PGC1) were analyzed in the heart using Western blot. Cardiac forkhead box transcription factor 1 (FOXO1) and sirtuin 1 (SIRT1) protein levels were also evaluated. Gene expression of long-chain acyl-CoA dehydrogenase was analyzed by quantitative polymerase chain reaction. Results: Exercise training has augmented the expression of main regulators of FA oxidation in the heart and achieves its effect by increasing the nuclear content of PPARα, Lipin1, and FOXO1 compared with the fructose group (P = 0.0422, P = 0.000045, P = 0.00958, respectively). In addition, Lipin1, FOXO1, and SIRT1 were increased in nuclear extract after exercise compared with the control group (P = 0.000043, P = 0.0417, P = 0.0329, respectively). In cardiac lysate, low-intensity exercise caused significantly increased protein level of PPARα, PGC1, FOXO1, and SIRT1 compared with control (P = 0.0377, P = 0.0275, P = 0.0096, P = 0.0282, respectively) and PGC1 level compared with the fructose group (P = 0.0417). Conclusion: The obtained results imply that the heart with a metabolic burden additionally relies on FA as an energy substrate after low-intensity running. © Copyright 2023, Mary Ann Liebert, Inc., publishers 2023.
T2  - Metabolic Syndrome and Related Disorders
T1  - Low-Intensity Exercise Affects Cardiac Fatty Acid Oxidation by Increasing the Nuclear Content of PPARα, FOXO1, and Lipin1 in Fructose-Fed Rats
VL  - 21
IS  - 2
SP  - 122
EP  - 131
DO  - 10.1089/met.2022.0078
ER  - 

@article{
author = "Kostić, Milan and Korićanac, Goran and Tepavčević, Snežana and Stanišić, Jelena and Romić, Snježana Đ. and Ćulafić, Tijana and Ivković, Tamara and Stojiljković, Mojca D.",
year = "2023",
abstract = "Background and Aim: Excessive fructose consumption along with a sedentary lifestyle provokes metabolic disorders and cardiovascular diseases. Fructose overload causes cardiac insulin resistance and increases reliance on fatty acid (FA) uptake and catabolism. The cardiometabolic benefits of exercise training have long been appreciated. The goal of the presented study is to shed a new light to the preventive role of exercise training on cardiac lipid metabolism in fructose-fed rats. Methods: Male Wistar rats were divided into control (C), sedentary fructose (F), and exercised fructose (EF) groups. Fructose was given as a 10% fructose solution in drinking water for 9 weeks. Low-intensity exercise training was applied for 9 weeks. The protein expression and subcellular localization of Lipin1, peroxisome proliferator-activated receptor α (PPARα), and peroxisome proliferator-activated receptor-γcoactivator 1 α (PGC1) were analyzed in the heart using Western blot. Cardiac forkhead box transcription factor 1 (FOXO1) and sirtuin 1 (SIRT1) protein levels were also evaluated. Gene expression of long-chain acyl-CoA dehydrogenase was analyzed by quantitative polymerase chain reaction. Results: Exercise training has augmented the expression of main regulators of FA oxidation in the heart and achieves its effect by increasing the nuclear content of PPARα, Lipin1, and FOXO1 compared with the fructose group (P = 0.0422, P = 0.000045, P = 0.00958, respectively). In addition, Lipin1, FOXO1, and SIRT1 were increased in nuclear extract after exercise compared with the control group (P = 0.000043, P = 0.0417, P = 0.0329, respectively). In cardiac lysate, low-intensity exercise caused significantly increased protein level of PPARα, PGC1, FOXO1, and SIRT1 compared with control (P = 0.0377, P = 0.0275, P = 0.0096, P = 0.0282, respectively) and PGC1 level compared with the fructose group (P = 0.0417). Conclusion: The obtained results imply that the heart with a metabolic burden additionally relies on FA as an energy substrate after low-intensity running. © Copyright 2023, Mary Ann Liebert, Inc., publishers 2023.",
journal = "Metabolic Syndrome and Related Disorders",
title = "Low-Intensity Exercise Affects Cardiac Fatty Acid Oxidation by Increasing the Nuclear Content of PPARα, FOXO1, and Lipin1 in Fructose-Fed Rats",
volume = "21",
number = "2",
pages = "122-131",
doi = "10.1089/met.2022.0078"
}

Kostić, M., Korićanac, G., Tepavčević, S., Stanišić, J., Romić, S. Đ., Ćulafić, T., Ivković, T.,& Stojiljković, M. D.. (2023). Low-Intensity Exercise Affects Cardiac Fatty Acid Oxidation by Increasing the Nuclear Content of PPARα, FOXO1, and Lipin1 in Fructose-Fed Rats. in Metabolic Syndrome and Related Disorders, 21(2), 122-131.
https://doi.org/10.1089/met.2022.0078

Kostić M, Korićanac G, Tepavčević S, Stanišić J, Romić SĐ, Ćulafić T, Ivković T, Stojiljković MD. Low-Intensity Exercise Affects Cardiac Fatty Acid Oxidation by Increasing the Nuclear Content of PPARα, FOXO1, and Lipin1 in Fructose-Fed Rats. in Metabolic Syndrome and Related Disorders. 2023;21(2):122-131.
doi:10.1089/met.2022.0078 .

Kostić, Milan, Korićanac, Goran, Tepavčević, Snežana, Stanišić, Jelena, Romić, Snježana Đ., Ćulafić, Tijana, Ivković, Tamara, Stojiljković, Mojca D., "Low-Intensity Exercise Affects Cardiac Fatty Acid Oxidation by Increasing the Nuclear Content of PPARα, FOXO1, and Lipin1 in Fructose-Fed Rats" in Metabolic Syndrome and Related Disorders, 21, no. 2 (2023):122-131,
https://doi.org/10.1089/met.2022.0078 . .

TY  - JOUR
AU  - Ivković, Tamara
AU  - Tepavčević, Snežana
AU  - Romić, Snježana Đ.
AU  - Stojiljković, Mojca D.
AU  - Kostić, Milan
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
AU  - Ćulafić, Tijana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10924
AB  - Cholecalciferol improves insulin signaling and glucose metabolism in the heart and reduces
circulating non-esterified fatty acids. Cholecalciferol effects on the cardiac fatty acid (FA) metabolism
and the consequences on calcium handling were examined. Blood lipid profile was determined.
Western blot and qRT-PCR were used to examine protein and mRNA expression. Cholecalciferoltreated rats had increased acetyl CoA carboxylase 2 protein expression and decreased expression of
malonyl CoA decarboxylase. In addition, the expression of uncoupling protein 3 was elevated. Also,
the level of peroxisome proliferator-activated receptor-gamma coactivator in the nucleus of heart
cells was increased along with the level of sarcoplasmic/endoplasmic reticulum Ca2+ATPase in the
microsomal fraction. In parallel, the L-type calcium channel and ryanodine receptor expression was
reduced. In the heart of healthy rats, cholecalciferol affects proteins regulating malonyl CoA availability and intracellular Ca2+ handling proteins.
T2  - General physiology and biophysics
T1  - Cholecalciferol affects cardiac proteins regulating malonyl-CoA availability and intracellular calcium level
VL  - 42
IS  - 03
SP  - 241
EP  - 250
DO  - 10.4149/gpb_2023005
ER  - 

@article{
author = "Ivković, Tamara and Tepavčević, Snežana and Romić, Snježana Đ. and Stojiljković, Mojca D. and Kostić, Milan and Stanišić, Jelena and Korićanac, Goran and Ćulafić, Tijana",
year = "2023",
abstract = "Cholecalciferol improves insulin signaling and glucose metabolism in the heart and reduces
circulating non-esterified fatty acids. Cholecalciferol effects on the cardiac fatty acid (FA) metabolism
and the consequences on calcium handling were examined. Blood lipid profile was determined.
Western blot and qRT-PCR were used to examine protein and mRNA expression. Cholecalciferoltreated rats had increased acetyl CoA carboxylase 2 protein expression and decreased expression of
malonyl CoA decarboxylase. In addition, the expression of uncoupling protein 3 was elevated. Also,
the level of peroxisome proliferator-activated receptor-gamma coactivator in the nucleus of heart
cells was increased along with the level of sarcoplasmic/endoplasmic reticulum Ca2+ATPase in the
microsomal fraction. In parallel, the L-type calcium channel and ryanodine receptor expression was
reduced. In the heart of healthy rats, cholecalciferol affects proteins regulating malonyl CoA availability and intracellular Ca2+ handling proteins.",
journal = "General physiology and biophysics",
title = "Cholecalciferol affects cardiac proteins regulating malonyl-CoA availability and intracellular calcium level",
volume = "42",
number = "03",
pages = "241-250",
doi = "10.4149/gpb_2023005"
}

Ivković, T., Tepavčević, S., Romić, S. Đ., Stojiljković, M. D., Kostić, M., Stanišić, J., Korićanac, G.,& Ćulafić, T.. (2023). Cholecalciferol affects cardiac proteins regulating malonyl-CoA availability and intracellular calcium level. in General physiology and biophysics, 42(03), 241-250.
https://doi.org/10.4149/gpb_2023005

Ivković T, Tepavčević S, Romić SĐ, Stojiljković MD, Kostić M, Stanišić J, Korićanac G, Ćulafić T. Cholecalciferol affects cardiac proteins regulating malonyl-CoA availability and intracellular calcium level. in General physiology and biophysics. 2023;42(03):241-250.
doi:10.4149/gpb_2023005 .

Ivković, Tamara, Tepavčević, Snežana, Romić, Snježana Đ., Stojiljković, Mojca D., Kostić, Milan, Stanišić, Jelena, Korićanac, Goran, Ćulafić, Tijana, "Cholecalciferol affects cardiac proteins regulating malonyl-CoA availability and intracellular calcium level" in General physiology and biophysics, 42, no. 03 (2023):241-250,
https://doi.org/10.4149/gpb_2023005 . .

TY  - JOUR
AU  - Tepavčević, Snežana
AU  - Romić, Snježana
AU  - Zec, Manja
AU  - Ćulafić, Tijana
AU  - Stojiljković, Mojca
AU  - Ivković, Tamara
AU  - Pantelić, Marija
AU  - Kostić, Milan
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11987
AB  - The transport of cations in the cardiomyocytes, crucial for the functioning of the heart, can be affected by walnut diet due to the high content of polyunsaturated fatty acids. Healthy and metabolically compromised rats (drinking 10% fructose solution) were subjected to a diet supplemented with 2.4 g of walnuts for 6 weeks to investigate the effect on proteins involved in cation transport in the heart cells. Fructose increased the level of the a1 subunit of Na+/K+-ATPase and the phosphorylation of extracellular signal-regulated kinase 1/2 in the heart of control and walnut-eating rats, while elevated L-type calcium channel a (LTCCa), sodium–calcium exchanger 1 (NCX1), and Maxi Ka level were observed only in rats that did not consume walnuts. However, walnuts significantly increased the cardiac content of LTCC, NCX1, and Maxi Ka, as well as Kir6.1 and SUR2B subunits of KATP channel, but only in fructose-naive rats. In animals that drank fructose, a significant increasing effect of walnuts was observed only in Akt kinase phosphorylation, which may be a part of the antiarrhythmic mechanism of decreasing cation currents in cardiomyocytes. The walnut diet-induced increase in LTCC and NCX1 expression in healthy rats may indicate intense cardiac calcium turnover, whereas the effect on Kir6.1 and SUR2B subunits suggests stimulation of KATP channel transport in the cardiac vasculature. The effects of walnuts on the cation-handling proteins in the heart, mostly limited to healthy animals, suggest the possible use of a walnut-supplemented diet in the prevention rather than the treatment of cardiological channelopathies.
T2  - Journal of Medicinal Food
T1  - Effects of Walnut-Rich Diet on Cation-Handling Proteins in the Heart of Healthy and Metabolically Compromised Male Rats
VL  - 26
IS  - 11
SP  - 849
EP  - 857
DO  - 10.1089/jmf.2022.0157
ER  - 

@article{
author = "Tepavčević, Snežana and Romić, Snježana and Zec, Manja and Ćulafić, Tijana and Stojiljković, Mojca and Ivković, Tamara and Pantelić, Marija and Kostić, Milan and Stanišić, Jelena and Korićanac, Goran",
year = "2023",
abstract = "The transport of cations in the cardiomyocytes, crucial for the functioning of the heart, can be affected by walnut diet due to the high content of polyunsaturated fatty acids. Healthy and metabolically compromised rats (drinking 10% fructose solution) were subjected to a diet supplemented with 2.4 g of walnuts for 6 weeks to investigate the effect on proteins involved in cation transport in the heart cells. Fructose increased the level of the a1 subunit of Na+/K+-ATPase and the phosphorylation of extracellular signal-regulated kinase 1/2 in the heart of control and walnut-eating rats, while elevated L-type calcium channel a (LTCCa), sodium–calcium exchanger 1 (NCX1), and Maxi Ka level were observed only in rats that did not consume walnuts. However, walnuts significantly increased the cardiac content of LTCC, NCX1, and Maxi Ka, as well as Kir6.1 and SUR2B subunits of KATP channel, but only in fructose-naive rats. In animals that drank fructose, a significant increasing effect of walnuts was observed only in Akt kinase phosphorylation, which may be a part of the antiarrhythmic mechanism of decreasing cation currents in cardiomyocytes. The walnut diet-induced increase in LTCC and NCX1 expression in healthy rats may indicate intense cardiac calcium turnover, whereas the effect on Kir6.1 and SUR2B subunits suggests stimulation of KATP channel transport in the cardiac vasculature. The effects of walnuts on the cation-handling proteins in the heart, mostly limited to healthy animals, suggest the possible use of a walnut-supplemented diet in the prevention rather than the treatment of cardiological channelopathies.",
journal = "Journal of Medicinal Food",
title = "Effects of Walnut-Rich Diet on Cation-Handling Proteins in the Heart of Healthy and Metabolically Compromised Male Rats",
volume = "26",
number = "11",
pages = "849-857",
doi = "10.1089/jmf.2022.0157"
}

Tepavčević, S., Romić, S., Zec, M., Ćulafić, T., Stojiljković, M., Ivković, T., Pantelić, M., Kostić, M., Stanišić, J.,& Korićanac, G.. (2023). Effects of Walnut-Rich Diet on Cation-Handling Proteins in the Heart of Healthy and Metabolically Compromised Male Rats. in Journal of Medicinal Food, 26(11), 849-857.
https://doi.org/10.1089/jmf.2022.0157

Tepavčević S, Romić S, Zec M, Ćulafić T, Stojiljković M, Ivković T, Pantelić M, Kostić M, Stanišić J, Korićanac G. Effects of Walnut-Rich Diet on Cation-Handling Proteins in the Heart of Healthy and Metabolically Compromised Male Rats. in Journal of Medicinal Food. 2023;26(11):849-857.
doi:10.1089/jmf.2022.0157 .

Tepavčević, Snežana, Romić, Snježana, Zec, Manja, Ćulafić, Tijana, Stojiljković, Mojca, Ivković, Tamara, Pantelić, Marija, Kostić, Milan, Stanišić, Jelena, Korićanac, Goran, "Effects of Walnut-Rich Diet on Cation-Handling Proteins in the Heart of Healthy and Metabolically Compromised Male Rats" in Journal of Medicinal Food, 26, no. 11 (2023):849-857,
https://doi.org/10.1089/jmf.2022.0157 . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Kirovski, Danijela
AU  - Vujanac, Ivan
AU  - Đorđević, Ana D.
AU  - Romić, Snježana Đ.
AU  - Pantelić, Marija
AU  - Korićanac, Goran
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10356
AB  - A number of alterations have been identified in lipid metabolism within adipose tissue and liver in obesity. Less is known about the capacity of skeletal muscle for the metabolism of fatty acids in obesity-related insulin resistance, though it is evident that dry cow muscles may contain increased triglyceride content. The current study was therefore undertaken to evaluate the skeletal muscle expression of proteins of the fatty acid metabolism in dry cows with different body condition scores (BCS). Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their BCS as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Blood samples collection and skeletal muscle biopsies were carried out at day 10 before calving. Blood serum was assayed for concentration of resistin using a bovine specific ELISA. Protein expression of insulin receptor beta subunit (IRβ), glucose transporter 4 (GLUT4), fatty acid translocase (FAT/CD36), fatty acid transporter 1 (FATP1), carnitine palmitoyltransferase 1 (CPT1), AMP-acitvated protein kinase (AMPK) and lipin 1 were analyzed in semitendinosus muscle by immunoblot. Resistin differed non-significantly between high-BCS and optimal-BCS cows. Insulin-resistant lipid metabolism in obese cows was paralleled with increased skeletal muscle expression of lipin 1 and GLUT4, and decreased expression of IRβ and FATP1. These data suggest that in obesity-related insulin resistance, metabolic capacity in dry cow skeletal muscles appears to be organized towards the synthesis of signaling intermediates rather than fatty acids oxidation and that altered fatty acid uptake does not contribute to this disposition.
T2  - Research in Veterinary Science
T1  - Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle
VL  - 150
SP  - 189
EP  - 194
DO  - 10.1016/j.rvsc.2022.04.012
ER  - 

@article{
author = "Prodanović, Radiša and Kirovski, Danijela and Vujanac, Ivan and Đorđević, Ana D. and Romić, Snježana Đ. and Pantelić, Marija and Korićanac, Goran",
year = "2022",
abstract = "A number of alterations have been identified in lipid metabolism within adipose tissue and liver in obesity. Less is known about the capacity of skeletal muscle for the metabolism of fatty acids in obesity-related insulin resistance, though it is evident that dry cow muscles may contain increased triglyceride content. The current study was therefore undertaken to evaluate the skeletal muscle expression of proteins of the fatty acid metabolism in dry cows with different body condition scores (BCS). Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their BCS as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Blood samples collection and skeletal muscle biopsies were carried out at day 10 before calving. Blood serum was assayed for concentration of resistin using a bovine specific ELISA. Protein expression of insulin receptor beta subunit (IRβ), glucose transporter 4 (GLUT4), fatty acid translocase (FAT/CD36), fatty acid transporter 1 (FATP1), carnitine palmitoyltransferase 1 (CPT1), AMP-acitvated protein kinase (AMPK) and lipin 1 were analyzed in semitendinosus muscle by immunoblot. Resistin differed non-significantly between high-BCS and optimal-BCS cows. Insulin-resistant lipid metabolism in obese cows was paralleled with increased skeletal muscle expression of lipin 1 and GLUT4, and decreased expression of IRβ and FATP1. These data suggest that in obesity-related insulin resistance, metabolic capacity in dry cow skeletal muscles appears to be organized towards the synthesis of signaling intermediates rather than fatty acids oxidation and that altered fatty acid uptake does not contribute to this disposition.",
journal = "Research in Veterinary Science",
title = "Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle",
volume = "150",
pages = "189-194",
doi = "10.1016/j.rvsc.2022.04.012"
}

Prodanović, R., Kirovski, D., Vujanac, I., Đorđević, A. D., Romić, S. Đ., Pantelić, M.,& Korićanac, G.. (2022). Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle. in Research in Veterinary Science, 150, 189-194.
https://doi.org/10.1016/j.rvsc.2022.04.012

Prodanović R, Kirovski D, Vujanac I, Đorđević AD, Romić SĐ, Pantelić M, Korićanac G. Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle. in Research in Veterinary Science. 2022;150:189-194.
doi:10.1016/j.rvsc.2022.04.012 .

Prodanović, Radiša, Kirovski, Danijela, Vujanac, Ivan, Đorđević, Ana D., Romić, Snježana Đ., Pantelić, Marija, Korićanac, Goran, "Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle" in Research in Veterinary Science, 150 (2022):189-194,
https://doi.org/10.1016/j.rvsc.2022.04.012 . .

TY  - JOUR
AU  - Romić, Snježana Đ.
AU  - Tepavčević, Snežana
AU  - Popović, Tamara
AU  - Zec, Manja
AU  - Stojiljković, Mojca D.
AU  - Ćulafić, Tijana
AU  - Bošković, Maja
AU  - Korićanac, Goran
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10391
AB  - Walnut consumption mostly has a positive implication for cardiovascular health. Walnut diet effects on the cardiac fatty acid (FA) metabolism of healthy rats and those with fructose diet-induced metabolic burden were analysed. Both walnuts and fructose increased CD36 transporter level and the nuclear content of some/all of Lipin 1/PPARα/PGC-1 complex partners, as well as cytosolic and nuclear FOXO1. However, fructose, independently of walnuts, increased the content of palmitic (PA), oleic, and vaccenic acid (VA), while in walnut-fed rats failed to increase palmitoleic acid (POA) level and the POA/PA ratio, as well as total MUFA content. In opposite, walnuts reduced the level of PA and VA and increased alpha-linolenic, eicosapentaenoic and docosapentaenoic acid level, regardless of fructose. In conclusion, both fructose and walnuts stimulated the uptake and oxidation of FA in the heart, but the walnuts, opposite to fructose, favourably altered cardiac FA profile in healthy and metabolically compromised rats.
T2  - International Journal of Food Sciences and Nutrition
T1  - Consumption of walnuts suppresses the conversion of palmitic to palmitoleic acid and enhances omega-3 fatty acid metabolism in the heart of fructose-fed rats
SP  - 1
EP  - 14
DO  - 10.1080/09637486.2022.2107186
ER  - 

@article{
author = "Romić, Snježana Đ. and Tepavčević, Snežana and Popović, Tamara and Zec, Manja and Stojiljković, Mojca D. and Ćulafić, Tijana and Bošković, Maja and Korićanac, Goran",
year = "2022",
abstract = "Walnut consumption mostly has a positive implication for cardiovascular health. Walnut diet effects on the cardiac fatty acid (FA) metabolism of healthy rats and those with fructose diet-induced metabolic burden were analysed. Both walnuts and fructose increased CD36 transporter level and the nuclear content of some/all of Lipin 1/PPARα/PGC-1 complex partners, as well as cytosolic and nuclear FOXO1. However, fructose, independently of walnuts, increased the content of palmitic (PA), oleic, and vaccenic acid (VA), while in walnut-fed rats failed to increase palmitoleic acid (POA) level and the POA/PA ratio, as well as total MUFA content. In opposite, walnuts reduced the level of PA and VA and increased alpha-linolenic, eicosapentaenoic and docosapentaenoic acid level, regardless of fructose. In conclusion, both fructose and walnuts stimulated the uptake and oxidation of FA in the heart, but the walnuts, opposite to fructose, favourably altered cardiac FA profile in healthy and metabolically compromised rats.",
journal = "International Journal of Food Sciences and Nutrition",
title = "Consumption of walnuts suppresses the conversion of palmitic to palmitoleic acid and enhances omega-3 fatty acid metabolism in the heart of fructose-fed rats",
pages = "1-14",
doi = "10.1080/09637486.2022.2107186"
}

Romić, S. Đ., Tepavčević, S., Popović, T., Zec, M., Stojiljković, M. D., Ćulafić, T., Bošković, M.,& Korićanac, G.. (2022). Consumption of walnuts suppresses the conversion of palmitic to palmitoleic acid and enhances omega-3 fatty acid metabolism in the heart of fructose-fed rats. in International Journal of Food Sciences and Nutrition, 1-14.
https://doi.org/10.1080/09637486.2022.2107186

Romić SĐ, Tepavčević S, Popović T, Zec M, Stojiljković MD, Ćulafić T, Bošković M, Korićanac G. Consumption of walnuts suppresses the conversion of palmitic to palmitoleic acid and enhances omega-3 fatty acid metabolism in the heart of fructose-fed rats. in International Journal of Food Sciences and Nutrition. 2022;:1-14.
doi:10.1080/09637486.2022.2107186 .

Romić, Snježana Đ., Tepavčević, Snežana, Popović, Tamara, Zec, Manja, Stojiljković, Mojca D., Ćulafić, Tijana, Bošković, Maja, Korićanac, Goran, "Consumption of walnuts suppresses the conversion of palmitic to palmitoleic acid and enhances omega-3 fatty acid metabolism in the heart of fructose-fed rats" in International Journal of Food Sciences and Nutrition (2022):1-14,
https://doi.org/10.1080/09637486.2022.2107186 . .

TY  - CONF
AU  - Kostić, Milan
AU  - Korićanac, Goran
AU  - Tepavčević, Snežana
AU  - Ćulafić, Tijana
AU  - Romić, Snježana Đ.
AU  - Stanišić, Jelena
AU  - Ivković, Tamara
AU  - Pantelić, Marija
AU  - Stojiljković, Mojca D.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11009
AB  - Excessive dietary fructose consumption in parallel with limited physical activity contributes to the global increase in prevalence of metabolic disorders. Metabolic syndrome represents a collection of cardiometabolic risk factors that includes obesity, insulin resistance, hypertension, and dyslipidemia, and it is undoubtedly linked to increased risk for two global maladies, type 2 diabetes, and cardiovascular diseases. Fructose-rich diet is accompanied by the development of insulin resistance in the heart, and it could change the use of cardiac energy substrates towards increased fatty acid (FA) uptake, and catabolism. Exercise may be beneficial in prevention and treatment of the metabolic syndrome. The aim of this study was to analyse the impact of low-intensity exercise on protein expression of nuclear transcription factors involved in regulating FA β- oxidation in a heart of fructose fed rats. Male Wistar rats were divided into control group, and two groups that received 10% fructose for 9 weeks, one which was sedentary and one which was additionally exposed to low intensity exercise. The protein expression of important transcriptional regulators of fatty acid β-oxidation PPARα, and FOXO1, and coregulators Lipin1, PGC-1, and SIRT1 are analyzed in cardiac lysate and/or nuclear fraction by Western blot. Gene expression of ACADL, the enzyme that catalyzes the initial step of mitochondrial β-oxidation, was quantified by real-time PCR. Fructose-rich diet decreased nuclear PPARα compared to control. Exercise increased nuclear PPARα, nuclear FOXO1, lysate PGC1, and nuclear Lipin1 in fructose-fed rats compared to sedentary fructose-fed rats. Exercise increased lysate PPARα, lysate and nuclear FOXO1, lysate PGC1, lysate and nuclear SIRT1, and nuclear Lipin1 in fructose-fed rats compared to control. In conclusion, running at low intensity is accompanied by increased expression of key regulators of fatty acid oxidation. The results indicate that exercise achieves its effect by increasing the nuclear content of PPARα, Lipin1, and FOXO1.
PB  - Belgrade : Faculty of Chemistry : Serbian Biochemical Society
C3  - Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia
T1  - Improvement of lipid metabolism regulation by low-intensity exercise in fructose-fed rats
SP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11009
ER  - 

@conference{
author = "Kostić, Milan and Korićanac, Goran and Tepavčević, Snežana and Ćulafić, Tijana and Romić, Snježana Đ. and Stanišić, Jelena and Ivković, Tamara and Pantelić, Marija and Stojiljković, Mojca D.",
year = "2022",
abstract = "Excessive dietary fructose consumption in parallel with limited physical activity contributes to the global increase in prevalence of metabolic disorders. Metabolic syndrome represents a collection of cardiometabolic risk factors that includes obesity, insulin resistance, hypertension, and dyslipidemia, and it is undoubtedly linked to increased risk for two global maladies, type 2 diabetes, and cardiovascular diseases. Fructose-rich diet is accompanied by the development of insulin resistance in the heart, and it could change the use of cardiac energy substrates towards increased fatty acid (FA) uptake, and catabolism. Exercise may be beneficial in prevention and treatment of the metabolic syndrome. The aim of this study was to analyse the impact of low-intensity exercise on protein expression of nuclear transcription factors involved in regulating FA β- oxidation in a heart of fructose fed rats. Male Wistar rats were divided into control group, and two groups that received 10% fructose for 9 weeks, one which was sedentary and one which was additionally exposed to low intensity exercise. The protein expression of important transcriptional regulators of fatty acid β-oxidation PPARα, and FOXO1, and coregulators Lipin1, PGC-1, and SIRT1 are analyzed in cardiac lysate and/or nuclear fraction by Western blot. Gene expression of ACADL, the enzyme that catalyzes the initial step of mitochondrial β-oxidation, was quantified by real-time PCR. Fructose-rich diet decreased nuclear PPARα compared to control. Exercise increased nuclear PPARα, nuclear FOXO1, lysate PGC1, and nuclear Lipin1 in fructose-fed rats compared to sedentary fructose-fed rats. Exercise increased lysate PPARα, lysate and nuclear FOXO1, lysate PGC1, lysate and nuclear SIRT1, and nuclear Lipin1 in fructose-fed rats compared to control. In conclusion, running at low intensity is accompanied by increased expression of key regulators of fatty acid oxidation. The results indicate that exercise achieves its effect by increasing the nuclear content of PPARα, Lipin1, and FOXO1.",
publisher = "Belgrade : Faculty of Chemistry : Serbian Biochemical Society",
journal = "Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia",
title = "Improvement of lipid metabolism regulation by low-intensity exercise in fructose-fed rats",
pages = "86",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11009"
}

Kostić, M., Korićanac, G., Tepavčević, S., Ćulafić, T., Romić, S. Đ., Stanišić, J., Ivković, T., Pantelić, M.,& Stojiljković, M. D.. (2022). Improvement of lipid metabolism regulation by low-intensity exercise in fructose-fed rats. in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia
Belgrade : Faculty of Chemistry : Serbian Biochemical Society., 86.
https://hdl.handle.net/21.15107/rcub_vinar_11009

Kostić M, Korićanac G, Tepavčević S, Ćulafić T, Romić SĐ, Stanišić J, Ivković T, Pantelić M, Stojiljković MD. Improvement of lipid metabolism regulation by low-intensity exercise in fructose-fed rats. in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia. 2022;:86.
https://hdl.handle.net/21.15107/rcub_vinar_11009 .

Kostić, Milan, Korićanac, Goran, Tepavčević, Snežana, Ćulafić, Tijana, Romić, Snježana Đ., Stanišić, Jelena, Ivković, Tamara, Pantelić, Marija, Stojiljković, Mojca D., "Improvement of lipid metabolism regulation by low-intensity exercise in fructose-fed rats" in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia (2022):86,
https://hdl.handle.net/21.15107/rcub_vinar_11009 .

TY  - JOUR
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
AU  - Ćulafić, Tijana
AU  - Romić, Snježana Đ.
AU  - Stojiljković, Mojca D.
AU  - Kostić, Milan
AU  - Ivković, Tamara
AU  - Tepavčević, Snežana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9919
AB  - We previously reported that low-intensity exercise prevented cardiac insulin resistance induced by a fructose-rich diet (FRD). To examine whether low-intensity exercise could prevent the disturbances of key molecules of cardiac glucose metabolism induced by FRD in male and ovariectomized (ovx) female rats, animals were exposed to 10% fructose solution (SF) or underwent both fructose diet and exercise (EF). Exercise prevented a decrease in cardiac GSK-3β phosphorylation induced by FRD in males (p <.001 vs. SF). It also prevented a decrease in PFK-2 phosphorylation in ovx females (p <.001 vs. SF) and increased the expression of PFK-2 in males (p <.05 vs. control). Exercise did not prevent a decrease in plasma membrane GLUT1 and GLUT4 levels in ovx females on FRD. The only effect of exercise on glucose transporters that could be indicated as beneficial is an augmented GLUT4 protein expression in males (p <.05 vs. control). Obtained results suggest that low-intensity exercise prevents harmful effects of FRD towards cardiac glycogenesis in males and glycolysis in ovx females. Practical applications: Low-intensity exercise, equivalent to brisk walking, was able to prevent disturbances in cardiac glycolysis regulation in ovx female and the glycogen synthesis pathway in male rats. In terms of human health, although molecular mechanisms of beneficial effects of exercise on cardiac glucose metabolism vary between genders, low-intensity running may be a useful non-pharmacological approach in the prevention of cardiac metabolic disorders in both men and postmenopausal women. © 2021 Wiley Periodicals LLC.
T2  - Journal of Food Biochemistry
T1  - The effects of low‐intensity exercise on cardiac glycogenesis and glycolysis in male and ovariectomized female rats on a fructose‐rich diet
VL  - 45
IS  - 10
DO  - 10.1111/jfbc.13930
ER  - 

@article{
author = "Stanišić, Jelena and Korićanac, Goran and Ćulafić, Tijana and Romić, Snježana Đ. and Stojiljković, Mojca D. and Kostić, Milan and Ivković, Tamara and Tepavčević, Snežana",
year = "2021",
abstract = "We previously reported that low-intensity exercise prevented cardiac insulin resistance induced by a fructose-rich diet (FRD). To examine whether low-intensity exercise could prevent the disturbances of key molecules of cardiac glucose metabolism induced by FRD in male and ovariectomized (ovx) female rats, animals were exposed to 10% fructose solution (SF) or underwent both fructose diet and exercise (EF). Exercise prevented a decrease in cardiac GSK-3β phosphorylation induced by FRD in males (p <.001 vs. SF). It also prevented a decrease in PFK-2 phosphorylation in ovx females (p <.001 vs. SF) and increased the expression of PFK-2 in males (p <.05 vs. control). Exercise did not prevent a decrease in plasma membrane GLUT1 and GLUT4 levels in ovx females on FRD. The only effect of exercise on glucose transporters that could be indicated as beneficial is an augmented GLUT4 protein expression in males (p <.05 vs. control). Obtained results suggest that low-intensity exercise prevents harmful effects of FRD towards cardiac glycogenesis in males and glycolysis in ovx females. Practical applications: Low-intensity exercise, equivalent to brisk walking, was able to prevent disturbances in cardiac glycolysis regulation in ovx female and the glycogen synthesis pathway in male rats. In terms of human health, although molecular mechanisms of beneficial effects of exercise on cardiac glucose metabolism vary between genders, low-intensity running may be a useful non-pharmacological approach in the prevention of cardiac metabolic disorders in both men and postmenopausal women. © 2021 Wiley Periodicals LLC.",
journal = "Journal of Food Biochemistry",
title = "The effects of low‐intensity exercise on cardiac glycogenesis and glycolysis in male and ovariectomized female rats on a fructose‐rich diet",
volume = "45",
number = "10",
doi = "10.1111/jfbc.13930"
}

Stanišić, J., Korićanac, G., Ćulafić, T., Romić, S. Đ., Stojiljković, M. D., Kostić, M., Ivković, T.,& Tepavčević, S.. (2021). The effects of low‐intensity exercise on cardiac glycogenesis and glycolysis in male and ovariectomized female rats on a fructose‐rich diet. in Journal of Food Biochemistry, 45(10).
https://doi.org/10.1111/jfbc.13930

Stanišić J, Korićanac G, Ćulafić T, Romić SĐ, Stojiljković MD, Kostić M, Ivković T, Tepavčević S. The effects of low‐intensity exercise on cardiac glycogenesis and glycolysis in male and ovariectomized female rats on a fructose‐rich diet. in Journal of Food Biochemistry. 2021;45(10).
doi:10.1111/jfbc.13930 .

Stanišić, Jelena, Korićanac, Goran, Ćulafić, Tijana, Romić, Snježana Đ., Stojiljković, Mojca D., Kostić, Milan, Ivković, Tamara, Tepavčević, Snežana, "The effects of low‐intensity exercise on cardiac glycogenesis and glycolysis in male and ovariectomized female rats on a fructose‐rich diet" in Journal of Food Biochemistry, 45, no. 10 (2021),
https://doi.org/10.1111/jfbc.13930 . .

TY  - JOUR
AU  - Ivković, Tamara
AU  - Čulafić, Tijana
AU  - Tepavčević, Snežana
AU  - Romić, Snježana Đ.
AU  - Stojiljković, Mojca D.
AU  - Kostić, Milan
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10054
AB  - Context The evidence on potential cross-talk of vitamin D and insulin in the regulation of cardiac metabolism is very scanty.Objective Cholecalciferol was administered to male Wistar rats for six weeks to study its effects on cardiac glucose metabolism regulation.Materials and methods An expression, phosphorylation and/or subcellular localisation of insulin signalling molecules, glucose transport and metabolism key proteins were studied.Results Circulating non-esterified fatty acids (NEFA) level was lower after cholecalciferol administration. Cholecalciferol decreased cardiac insulin receptor substrate 1 Ser307 phosphorylation, while insulin-stimulated Akt Thr308 phosphorylation was increased. Cardiac 6-phosphofructo-2-kinase protein, hexokinase 2 mRNA level and insulin-stimulated glycogen synthase kinase 3β Ser9 phosphorylation were also increased. Finally, FOXO1 transcription factor cytosolic level was reduced.Conclusion Vitamin D-related improvement of insulin signalling and insulin regulation of glucose metabolism in the rat heart is accompanied by the decrease of blood NEFA level and dysregulation of cardiac FOXO1 signalling.
T2  - Archives of Physiology and Biochemistry
T1  - Cholecalciferol ameliorates insulin signalling and insulin regulation of enzymes involved in glucose metabolism in the rat heart
SP  - 1
EP  - 9
DO  - 10.1080/13813455.2021.2001020
ER  - 

@article{
author = "Ivković, Tamara and Čulafić, Tijana and Tepavčević, Snežana and Romić, Snježana Đ. and Stojiljković, Mojca D. and Kostić, Milan and Stanišić, Jelena and Korićanac, Goran",
year = "2021",
abstract = "Context The evidence on potential cross-talk of vitamin D and insulin in the regulation of cardiac metabolism is very scanty.Objective Cholecalciferol was administered to male Wistar rats for six weeks to study its effects on cardiac glucose metabolism regulation.Materials and methods An expression, phosphorylation and/or subcellular localisation of insulin signalling molecules, glucose transport and metabolism key proteins were studied.Results Circulating non-esterified fatty acids (NEFA) level was lower after cholecalciferol administration. Cholecalciferol decreased cardiac insulin receptor substrate 1 Ser307 phosphorylation, while insulin-stimulated Akt Thr308 phosphorylation was increased. Cardiac 6-phosphofructo-2-kinase protein, hexokinase 2 mRNA level and insulin-stimulated glycogen synthase kinase 3β Ser9 phosphorylation were also increased. Finally, FOXO1 transcription factor cytosolic level was reduced.Conclusion Vitamin D-related improvement of insulin signalling and insulin regulation of glucose metabolism in the rat heart is accompanied by the decrease of blood NEFA level and dysregulation of cardiac FOXO1 signalling.",
journal = "Archives of Physiology and Biochemistry",
title = "Cholecalciferol ameliorates insulin signalling and insulin regulation of enzymes involved in glucose metabolism in the rat heart",
pages = "1-9",
doi = "10.1080/13813455.2021.2001020"
}

Ivković, T., Čulafić, T., Tepavčević, S., Romić, S. Đ., Stojiljković, M. D., Kostić, M., Stanišić, J.,& Korićanac, G.. (2021). Cholecalciferol ameliorates insulin signalling and insulin regulation of enzymes involved in glucose metabolism in the rat heart. in Archives of Physiology and Biochemistry, 1-9.
https://doi.org/10.1080/13813455.2021.2001020

Ivković T, Čulafić T, Tepavčević S, Romić SĐ, Stojiljković MD, Kostić M, Stanišić J, Korićanac G. Cholecalciferol ameliorates insulin signalling and insulin regulation of enzymes involved in glucose metabolism in the rat heart. in Archives of Physiology and Biochemistry. 2021;:1-9.
doi:10.1080/13813455.2021.2001020 .

Ivković, Tamara, Čulafić, Tijana, Tepavčević, Snežana, Romić, Snježana Đ., Stojiljković, Mojca D., Kostić, Milan, Stanišić, Jelena, Korićanac, Goran, "Cholecalciferol ameliorates insulin signalling and insulin regulation of enzymes involved in glucose metabolism in the rat heart" in Archives of Physiology and Biochemistry (2021):1-9,
https://doi.org/10.1080/13813455.2021.2001020 . .

TY  - JOUR
AU  - Stanišić, Jelena
AU  - Ivković, Tamara
AU  - Romić, Snježana Đ.
AU  - Zec, Manja
AU  - Ćulafić, Tijana
AU  - Stojiljković, Mojca D.
AU  - Korićanac, Goran
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9103
AB  - Consumption of walnuts is beneficial for cardiovascular health. To study walnut effects on proteins involved in vascular tone regulation, control and fructose-fed rats were subjected to walnut diet for 6 weeks. In contrast with increased energy intake and body mass gain, aortic protein level of L-type calcium channel alpha subunit was decreased and the level of SUR2B subunit of ATP-sensitive K + channel was increased in healthy rats subjected to walnuts, together with improved Akt phosphorylation. Upon the walnut diet in rats subjected to fructose overload, the rise in energy intake and body mass gain, was followed by an increase in blood insulin. Although SUR2B level was elevated, the level of sodium-calcium exchanger NCX1 and inducible nitric oxide synthase were reduced and increased, respectively. In summary, walnut consumption was accompanied with moderate beneficial vascular effect in healthy rats, while an effect of walnut in rats with metabolic disturbances was rather controversial.
T2  - International Journal of Food Sciences and Nutrition
T1  - Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2
VL  - 72
IS  - 3
SP  - 324
EP  - 334
DO  - 10.1080/09637486.2020.1796931
ER  - 

@article{
author = "Stanišić, Jelena and Ivković, Tamara and Romić, Snježana Đ. and Zec, Manja and Ćulafić, Tijana and Stojiljković, Mojca D. and Korićanac, Goran",
year = "2021",
abstract = "Consumption of walnuts is beneficial for cardiovascular health. To study walnut effects on proteins involved in vascular tone regulation, control and fructose-fed rats were subjected to walnut diet for 6 weeks. In contrast with increased energy intake and body mass gain, aortic protein level of L-type calcium channel alpha subunit was decreased and the level of SUR2B subunit of ATP-sensitive K + channel was increased in healthy rats subjected to walnuts, together with improved Akt phosphorylation. Upon the walnut diet in rats subjected to fructose overload, the rise in energy intake and body mass gain, was followed by an increase in blood insulin. Although SUR2B level was elevated, the level of sodium-calcium exchanger NCX1 and inducible nitric oxide synthase were reduced and increased, respectively. In summary, walnut consumption was accompanied with moderate beneficial vascular effect in healthy rats, while an effect of walnut in rats with metabolic disturbances was rather controversial.",
journal = "International Journal of Food Sciences and Nutrition",
title = "Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2",
volume = "72",
number = "3",
pages = "324-334",
doi = "10.1080/09637486.2020.1796931"
}

Stanišić, J., Ivković, T., Romić, S. Đ., Zec, M., Ćulafić, T., Stojiljković, M. D.,& Korićanac, G.. (2021). Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2. in International Journal of Food Sciences and Nutrition, 72(3), 324-334.
https://doi.org/10.1080/09637486.2020.1796931

Stanišić J, Ivković T, Romić SĐ, Zec M, Ćulafić T, Stojiljković MD, Korićanac G. Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2. in International Journal of Food Sciences and Nutrition. 2021;72(3):324-334.
doi:10.1080/09637486.2020.1796931 .

Stanišić, Jelena, Ivković, Tamara, Romić, Snježana Đ., Zec, Manja, Ćulafić, Tijana, Stojiljković, Mojca D., Korićanac, Goran, "Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2" in International Journal of Food Sciences and Nutrition, 72, no. 3 (2021):324-334,
https://doi.org/10.1080/09637486.2020.1796931 . .

TY  - JOUR
AU  - Stanišić, Jelena
AU  - Ivković, Tamara
AU  - Romić, Snježana Đ.
AU  - Zec, Manja
AU  - Ćulafić, Tijana
AU  - Stojiljković, Mojca D.
AU  - Korićanac, Goran
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9110
AB  - Consumption of walnuts is beneficial for cardiovascular health. To study walnut effects on proteins involved in vascular tone regulation, control and fructose-fed rats were subjected to walnut diet for 6 weeks. In contrast with increased energy intake and body mass gain, aortic protein level of L-type calcium channel alpha subunit was decreased and the level of SUR2B subunit of ATP-sensitive K + channel was increased in healthy rats subjected to walnuts, together with improved Akt phosphorylation. Upon the walnut diet in rats subjected to fructose overload, the rise in energy intake and body mass gain, was followed by an increase in blood insulin. Although SUR2B level was elevated, the level of sodium-calcium exchanger NCX1 and inducible nitric oxide synthase were reduced and increased, respectively. In summary, walnut consumption was accompanied with moderate beneficial vascular effect in healthy rats, while an effect of walnut in rats with metabolic disturbances was rather controversial.
T2  - International Journal of Food Sciences and Nutrition
T1  - Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2
VL  - 72
IS  - 3
SP  - 324
EP  - 334
DO  - 10.1080/09637486.2020.1796931
ER  - 

@article{
author = "Stanišić, Jelena and Ivković, Tamara and Romić, Snježana Đ. and Zec, Manja and Ćulafić, Tijana and Stojiljković, Mojca D. and Korićanac, Goran",
year = "2021",
abstract = "Consumption of walnuts is beneficial for cardiovascular health. To study walnut effects on proteins involved in vascular tone regulation, control and fructose-fed rats were subjected to walnut diet for 6 weeks. In contrast with increased energy intake and body mass gain, aortic protein level of L-type calcium channel alpha subunit was decreased and the level of SUR2B subunit of ATP-sensitive K + channel was increased in healthy rats subjected to walnuts, together with improved Akt phosphorylation. Upon the walnut diet in rats subjected to fructose overload, the rise in energy intake and body mass gain, was followed by an increase in blood insulin. Although SUR2B level was elevated, the level of sodium-calcium exchanger NCX1 and inducible nitric oxide synthase were reduced and increased, respectively. In summary, walnut consumption was accompanied with moderate beneficial vascular effect in healthy rats, while an effect of walnut in rats with metabolic disturbances was rather controversial.",
journal = "International Journal of Food Sciences and Nutrition",
title = "Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2",
volume = "72",
number = "3",
pages = "324-334",
doi = "10.1080/09637486.2020.1796931"
}

Stanišić, J., Ivković, T., Romić, S. Đ., Zec, M., Ćulafić, T., Stojiljković, M. D.,& Korićanac, G.. (2021). Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2. in International Journal of Food Sciences and Nutrition, 72(3), 324-334.
https://doi.org/10.1080/09637486.2020.1796931

Stanišić J, Ivković T, Romić SĐ, Zec M, Ćulafić T, Stojiljković MD, Korićanac G. Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2. in International Journal of Food Sciences and Nutrition. 2021;72(3):324-334.
doi:10.1080/09637486.2020.1796931 .

Stanišić, Jelena, Ivković, Tamara, Romić, Snježana Đ., Zec, Manja, Ćulafić, Tijana, Stojiljković, Mojca D., Korićanac, Goran, "Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2" in International Journal of Food Sciences and Nutrition, 72, no. 3 (2021):324-334,
https://doi.org/10.1080/09637486.2020.1796931 . .

TY  - JOUR
AU  - Romić, Snježana Đ.
AU  - Đorđević, Ana
AU  - Tepavčević, Snežana
AU  - Ćulafić, Tijana
AU  - Stojiljković, Mojca D.
AU  - Bursać, Biljana
AU  - Stanišić, Jelena
AU  - Kostić, Milan
AU  - Gligorovska, Ljupka
AU  - Korićanac, Goran
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8849
AB  - Both a diet rich in fructose and chronic stress exposure induce metabolic and cardiovascular disturbances. The aim of this study was to examine the effects of the fructose-rich diet and chronic stress, separately and in combination, on insulin signaling and molecules regulating glycogen synthesis and ion transport in the heart, and to reveal whether these effects coincide with changes in glucocorticoid receptor (GR) activation. Male Wistar rats were subjected to 10% fructose in drinking water and/or to chronic unpredictable stress for 9 weeks. Protein expression and/or phosphorylation of the insulin receptor (IR), protein tyrosine phosphatase 1B, insulin receptor substrate 1 (IRS1), protein kinase B (Akt), extracellular signal-regulated kinase 1/2 (ERK1/2), glycogen synthase kinase-3β (GSK-3β) and Na+/K+-ATPase α-subunits in cardiac tissue were analyzed by western blot. GR distribution between cytosolic and nuclear fractions was also analyzed. The fructose-rich diet decreased the level of pERK1/2 (Thr202/Tyr204) and pGSK-3β (Ser9) independently of stress, while chronic stress increased the IRS1 content and prevented the fructose diet-induced decrease of the pAkt (Ser473) level. The fructose-rich diet in combination with chronic stress reduced the protein content of cardiac IR and attenuated IRS1 upregulation. Separate treatments increased the protein content of Na+/K+-ATPase α1- and α2-subunits, while after combined treatment the α2 content was at the control level and the α1 content was lower than the control level. The effect of combined treatment on cardiac IR and α2-subunit expression could be mediated by increased GR nuclear accumulation. Our study provides new insights into the effects of chronic stress and a combination of the fructose diet and chronic stress on the studied molecules in the heart.
T2  - Food and Function
T1  - Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats
VL  - 11
IS  - 2
SP  - 1455
EP  - 1466
DO  - 10.1039/C9FO02306B
ER  - 

@article{
author = "Romić, Snježana Đ. and Đorđević, Ana and Tepavčević, Snežana and Ćulafić, Tijana and Stojiljković, Mojca D. and Bursać, Biljana and Stanišić, Jelena and Kostić, Milan and Gligorovska, Ljupka and Korićanac, Goran",
year = "2020",
abstract = "Both a diet rich in fructose and chronic stress exposure induce metabolic and cardiovascular disturbances. The aim of this study was to examine the effects of the fructose-rich diet and chronic stress, separately and in combination, on insulin signaling and molecules regulating glycogen synthesis and ion transport in the heart, and to reveal whether these effects coincide with changes in glucocorticoid receptor (GR) activation. Male Wistar rats were subjected to 10% fructose in drinking water and/or to chronic unpredictable stress for 9 weeks. Protein expression and/or phosphorylation of the insulin receptor (IR), protein tyrosine phosphatase 1B, insulin receptor substrate 1 (IRS1), protein kinase B (Akt), extracellular signal-regulated kinase 1/2 (ERK1/2), glycogen synthase kinase-3β (GSK-3β) and Na+/K+-ATPase α-subunits in cardiac tissue were analyzed by western blot. GR distribution between cytosolic and nuclear fractions was also analyzed. The fructose-rich diet decreased the level of pERK1/2 (Thr202/Tyr204) and pGSK-3β (Ser9) independently of stress, while chronic stress increased the IRS1 content and prevented the fructose diet-induced decrease of the pAkt (Ser473) level. The fructose-rich diet in combination with chronic stress reduced the protein content of cardiac IR and attenuated IRS1 upregulation. Separate treatments increased the protein content of Na+/K+-ATPase α1- and α2-subunits, while after combined treatment the α2 content was at the control level and the α1 content was lower than the control level. The effect of combined treatment on cardiac IR and α2-subunit expression could be mediated by increased GR nuclear accumulation. Our study provides new insights into the effects of chronic stress and a combination of the fructose diet and chronic stress on the studied molecules in the heart.",
journal = "Food and Function",
title = "Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats",
volume = "11",
number = "2",
pages = "1455-1466",
doi = "10.1039/C9FO02306B"
}

Romić, S. Đ., Đorđević, A., Tepavčević, S., Ćulafić, T., Stojiljković, M. D., Bursać, B., Stanišić, J., Kostić, M., Gligorovska, L.,& Korićanac, G.. (2020). Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats. in Food and Function, 11(2), 1455-1466.
https://doi.org/10.1039/C9FO02306B

Romić SĐ, Đorđević A, Tepavčević S, Ćulafić T, Stojiljković MD, Bursać B, Stanišić J, Kostić M, Gligorovska L, Korićanac G. Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats. in Food and Function. 2020;11(2):1455-1466.
doi:10.1039/C9FO02306B .

Romić, Snježana Đ., Đorđević, Ana, Tepavčević, Snežana, Ćulafić, Tijana, Stojiljković, Mojca D., Bursać, Biljana, Stanišić, Jelena, Kostić, Milan, Gligorovska, Ljupka, Korićanac, Goran, "Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats" in Food and Function, 11, no. 2 (2020):1455-1466,
https://doi.org/10.1039/C9FO02306B . .

TY  - CONF
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
AU  - Stojiljković, Mirjana
AU  - Ćulafić, Tijana
AU  - Kostić, Mirjana M.
AU  - Romić, Snježana Đ.
AU  - Pantelić, Marija
AU  - Tepavčević, Snežana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7920
C3  - FEBS OPEN BIO
T1  - Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats
VL  - 8
IS  - Supplement 1
SP  - 224
DO  - 10.1002/2211-5463.12453
ER  - 

@conference{
author = "Stanišić, Jelena and Korićanac, Goran and Stojiljković, Mirjana and Ćulafić, Tijana and Kostić, Mirjana M. and Romić, Snježana Đ. and Pantelić, Marija and Tepavčević, Snežana",
year = "2018",
journal = "FEBS OPEN BIO",
title = "Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats",
volume = "8",
number = "Supplement 1",
pages = "224",
doi = "10.1002/2211-5463.12453"
}

Stanišić, J., Korićanac, G., Stojiljković, M., Ćulafić, T., Kostić, M. M., Romić, S. Đ., Pantelić, M.,& Tepavčević, S.. (2018). Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats. in FEBS OPEN BIO, 8(Supplement 1), 224.
https://doi.org/10.1002/2211-5463.12453

Stanišić J, Korićanac G, Stojiljković M, Ćulafić T, Kostić MM, Romić SĐ, Pantelić M, Tepavčević S. Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats. in FEBS OPEN BIO. 2018;8(Supplement 1):224.
doi:10.1002/2211-5463.12453 .

Stanišić, Jelena, Korićanac, Goran, Stojiljković, Mirjana, Ćulafić, Tijana, Kostić, Mirjana M., Romić, Snježana Đ., Pantelić, Marija, Tepavčević, Snežana, "Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats" in FEBS OPEN BIO, 8, no. Supplement 1 (2018):224,
https://doi.org/10.1002/2211-5463.12453 . .

TY  - CONF
AU  - Kostić, Milan
AU  - Korićanac, Goran
AU  - Tepavčević, Snežana
AU  - Ćulafić, Tijana
AU  - Romić, Snježana Đ.
AU  - Stanišić, Jelena
AU  - Pantelić, Marija
AU  - Stojiljković, Mirjana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7922
C3  - FEBS OPEN BIO
T1  - Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet
VL  - 8
IS  - Supplement 1
SP  - 223
DO  - 10.1002/2211-5463.12453
ER  - 

@conference{
author = "Kostić, Milan and Korićanac, Goran and Tepavčević, Snežana and Ćulafić, Tijana and Romić, Snježana Đ. and Stanišić, Jelena and Pantelić, Marija and Stojiljković, Mirjana",
year = "2018",
journal = "FEBS OPEN BIO",
title = "Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet",
volume = "8",
number = "Supplement 1",
pages = "223",
doi = "10.1002/2211-5463.12453"
}

Kostić, M., Korićanac, G., Tepavčević, S., Ćulafić, T., Romić, S. Đ., Stanišić, J., Pantelić, M.,& Stojiljković, M.. (2018). Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet. in FEBS OPEN BIO, 8(Supplement 1), 223.
https://doi.org/10.1002/2211-5463.12453

Kostić M, Korićanac G, Tepavčević S, Ćulafić T, Romić SĐ, Stanišić J, Pantelić M, Stojiljković M. Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet. in FEBS OPEN BIO. 2018;8(Supplement 1):223.
doi:10.1002/2211-5463.12453 .

Kostić, Milan, Korićanac, Goran, Tepavčević, Snežana, Ćulafić, Tijana, Romić, Snježana Đ., Stanišić, Jelena, Pantelić, Marija, Stojiljković, Mirjana, "Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet" in FEBS OPEN BIO, 8, no. Supplement 1 (2018):223,
https://doi.org/10.1002/2211-5463.12453 . .

TY  - CONF
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
AU  - Stojiljković, Mojca D.
AU  - Ćulafić, Tijana
AU  - Romić, Snježana Đ.
AU  - Kostić, Milan
AU  - Pantelić, M.
AU  - Tepavčević, Snežana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7945
C3  - Atherosclerosis
T1  - Disturbances in cardiac insulin signaling and nitric oxide synthase in ovariectomized rats on fructose diet can be prevented by low intensity exercise
VL  - 275
SP  - e197
DO  - 10.1016/j.atherosclerosis.2018.06.607
ER  - 

@conference{
author = "Stanišić, Jelena and Korićanac, Goran and Stojiljković, Mojca D. and Ćulafić, Tijana and Romić, Snježana Đ. and Kostić, Milan and Pantelić, M. and Tepavčević, Snežana",
year = "2018",
journal = "Atherosclerosis",
title = "Disturbances in cardiac insulin signaling and nitric oxide synthase in ovariectomized rats on fructose diet can be prevented by low intensity exercise",
volume = "275",
pages = "e197",
doi = "10.1016/j.atherosclerosis.2018.06.607"
}

Stanišić, J., Korićanac, G., Stojiljković, M. D., Ćulafić, T., Romić, S. Đ., Kostić, M., Pantelić, M.,& Tepavčević, S.. (2018). Disturbances in cardiac insulin signaling and nitric oxide synthase in ovariectomized rats on fructose diet can be prevented by low intensity exercise. in Atherosclerosis, 275, e197.
https://doi.org/10.1016/j.atherosclerosis.2018.06.607

Stanišić J, Korićanac G, Stojiljković MD, Ćulafić T, Romić SĐ, Kostić M, Pantelić M, Tepavčević S. Disturbances in cardiac insulin signaling and nitric oxide synthase in ovariectomized rats on fructose diet can be prevented by low intensity exercise. in Atherosclerosis. 2018;275:e197.
doi:10.1016/j.atherosclerosis.2018.06.607 .

Stanišić, Jelena, Korićanac, Goran, Stojiljković, Mojca D., Ćulafić, Tijana, Romić, Snježana Đ., Kostić, Milan, Pantelić, M., Tepavčević, Snežana, "Disturbances in cardiac insulin signaling and nitric oxide synthase in ovariectomized rats on fructose diet can be prevented by low intensity exercise" in Atherosclerosis, 275 (2018):e197,
https://doi.org/10.1016/j.atherosclerosis.2018.06.607 . .

TY  - JOUR
AU  - Bundalo, Maja M.
AU  - Romić, Snježana Đ.
AU  - Tepavčević, Snežana
AU  - Stojiljković, Mojca D.
AU  - Stanković, Aleksandra
AU  - Živković, Maja
AU  - Korićanac, Goran
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1726
AB  - Increased intake of fructose in humans and laboratory animals is demonstrated to be a risk factor for development of metabolic disorders (insulin resistance, metabolic syndrome, type 2 diabetes) and cardiovascular diseases. On the other hand, estradiol is emphasized as a cardioprotective agent. The main goal of this review is to summarize recent findings on damaging cardiac effects of fructose-rich diet in females, mostly experimental animals, and to evaluate protective capacity of estradiol. Published results of our and other research groups indicate mostly detrimental effects of fructose-rich diet on cardiac insulin signaling molecules, glucose and fatty acid metabolism, nitric oxide production and ion transport, as well as renin-angiotensin system and inflammation. Some of these processes are involved in cardiac insulin signal transmission, others are regulated by insulin or have an influence on insulin action. Administration of estradiol to ovariectomized female rats, exposed to increased intake of fructose, was mostly beneficial to the heart, but sometimes it was ineffective or even detrimental, depending on the particular processes. We believe that these data, carefully translated to human population, could be useful for clinicians dealing with postmenopausal women susceptible to metabolic diseases and hormone replacement therapy.
T2  - European Journal of Pharmacology
T1  - Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?
VL  - 811
SP  - 141
EP  - 147
DO  - 10.1016/j.ejphar.2017.06.003
ER  - 

@article{
author = "Bundalo, Maja M. and Romić, Snježana Đ. and Tepavčević, Snežana and Stojiljković, Mojca D. and Stanković, Aleksandra and Živković, Maja and Korićanac, Goran",
year = "2017",
abstract = "Increased intake of fructose in humans and laboratory animals is demonstrated to be a risk factor for development of metabolic disorders (insulin resistance, metabolic syndrome, type 2 diabetes) and cardiovascular diseases. On the other hand, estradiol is emphasized as a cardioprotective agent. The main goal of this review is to summarize recent findings on damaging cardiac effects of fructose-rich diet in females, mostly experimental animals, and to evaluate protective capacity of estradiol. Published results of our and other research groups indicate mostly detrimental effects of fructose-rich diet on cardiac insulin signaling molecules, glucose and fatty acid metabolism, nitric oxide production and ion transport, as well as renin-angiotensin system and inflammation. Some of these processes are involved in cardiac insulin signal transmission, others are regulated by insulin or have an influence on insulin action. Administration of estradiol to ovariectomized female rats, exposed to increased intake of fructose, was mostly beneficial to the heart, but sometimes it was ineffective or even detrimental, depending on the particular processes. We believe that these data, carefully translated to human population, could be useful for clinicians dealing with postmenopausal women susceptible to metabolic diseases and hormone replacement therapy.",
journal = "European Journal of Pharmacology",
title = "Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?",
volume = "811",
pages = "141-147",
doi = "10.1016/j.ejphar.2017.06.003"
}

Bundalo, M. M., Romić, S. Đ., Tepavčević, S., Stojiljković, M. D., Stanković, A., Živković, M.,& Korićanac, G.. (2017). Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?. in European Journal of Pharmacology, 811, 141-147.
https://doi.org/10.1016/j.ejphar.2017.06.003

Bundalo MM, Romić SĐ, Tepavčević S, Stojiljković MD, Stanković A, Živković M, Korićanac G. Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?. in European Journal of Pharmacology. 2017;811:141-147.
doi:10.1016/j.ejphar.2017.06.003 .

Bundalo, Maja M., Romić, Snježana Đ., Tepavčević, Snežana, Stojiljković, Mojca D., Stanković, Aleksandra, Živković, Maja, Korićanac, Goran, "Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?" in European Journal of Pharmacology, 811 (2017):141-147,
https://doi.org/10.1016/j.ejphar.2017.06.003 . .

TY  - JOUR
AU  - Romić, Snježana Đ.
AU  - Krskova, Katarina
AU  - Olszanecki, Rafal
AU  - Balazova, Lucia
AU  - Lory, Viktoria
AU  - Korićanac, Goran
AU  - Slamkova, Miroslava
AU  - Zorad, Stefan
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1764
AB  - Fatty acid (FA) uptake and/or intramuscular triglyceride (TG) accumulation in skeletal muscle are increased in obesity, type 2 diabetes and aging. FA translocase (FAT/CD36) translocation, lipin-1 subcellular localization and nuclear factor kappa B (NF-kappa B) p65 protein content in quadriceps muscle of young and old obese Zuckerfa/fa rats and their lean controls were analyzed by immunoblot to define obesity- and aging-related alterations in FA uptake, their subsequent metabolic fate and potential to activate pro-inflammatory signaling. As expected, obesity increased FAT/CD36 content in plasma membrane in quadriceps muscle of fa/fa rats. Aging increased cytosolic lipin-1 content in both, obese rats and their lean controls. Also, old obese rats had decreased level of nuclear extract lipin-lcompared to that in old lean rats. Neither obesity nor age altered NF-kappa B p65 protein content in cytosol and nuclear extract of quadriceps muscle suggesting that obesity/aging-induced changes in FA handling are not accompanied by NF-kappa B-mediated inflammation. Increase in plasma membrane FAT/CD36 content in obese rats and failure in lipin-1 export to nucleus with progression of obesity, implying an increase in FA uptake and their different channeling into lipid intermediates synthesis pathway in old fa/fa rats versus FA usage in lean rats of the same age.
T2  - General Physiology and Biophysics
T1  - Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats
VL  - 36
IS  - 4
SP  - 399
EP  - 406
DO  - 10.4149/gpb_2017010
ER  - 

@article{
author = "Romić, Snježana Đ. and Krskova, Katarina and Olszanecki, Rafal and Balazova, Lucia and Lory, Viktoria and Korićanac, Goran and Slamkova, Miroslava and Zorad, Stefan",
year = "2017",
abstract = "Fatty acid (FA) uptake and/or intramuscular triglyceride (TG) accumulation in skeletal muscle are increased in obesity, type 2 diabetes and aging. FA translocase (FAT/CD36) translocation, lipin-1 subcellular localization and nuclear factor kappa B (NF-kappa B) p65 protein content in quadriceps muscle of young and old obese Zuckerfa/fa rats and their lean controls were analyzed by immunoblot to define obesity- and aging-related alterations in FA uptake, their subsequent metabolic fate and potential to activate pro-inflammatory signaling. As expected, obesity increased FAT/CD36 content in plasma membrane in quadriceps muscle of fa/fa rats. Aging increased cytosolic lipin-1 content in both, obese rats and their lean controls. Also, old obese rats had decreased level of nuclear extract lipin-lcompared to that in old lean rats. Neither obesity nor age altered NF-kappa B p65 protein content in cytosol and nuclear extract of quadriceps muscle suggesting that obesity/aging-induced changes in FA handling are not accompanied by NF-kappa B-mediated inflammation. Increase in plasma membrane FAT/CD36 content in obese rats and failure in lipin-1 export to nucleus with progression of obesity, implying an increase in FA uptake and their different channeling into lipid intermediates synthesis pathway in old fa/fa rats versus FA usage in lean rats of the same age.",
journal = "General Physiology and Biophysics",
title = "Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats",
volume = "36",
number = "4",
pages = "399-406",
doi = "10.4149/gpb_2017010"
}

Romić, S. Đ., Krskova, K., Olszanecki, R., Balazova, L., Lory, V., Korićanac, G., Slamkova, M.,& Zorad, S.. (2017). Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats. in General Physiology and Biophysics, 36(4), 399-406.
https://doi.org/10.4149/gpb_2017010

Romić SĐ, Krskova K, Olszanecki R, Balazova L, Lory V, Korićanac G, Slamkova M, Zorad S. Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats. in General Physiology and Biophysics. 2017;36(4):399-406.
doi:10.4149/gpb_2017010 .

Romić, Snježana Đ., Krskova, Katarina, Olszanecki, Rafal, Balazova, Lucia, Lory, Viktoria, Korićanac, Goran, Slamkova, Miroslava, Zorad, Stefan, "Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats" in General Physiology and Biophysics, 36, no. 4 (2017):399-406,
https://doi.org/10.4149/gpb_2017010 . .

TY  - JOUR
AU  - Bundalo, Maja M.
AU  - Živković, Maja
AU  - Romić, Snježana Đ.
AU  - Tepavčević, Snežana
AU  - Korićanac, Goran
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1132
AB  - Introduction: The cardiovascular renin-angiotensin system (RAS) could be affected by gender and dietary regime. We hypothesized that male rats will be more susceptible to activation of RAS in the heart and aorta, as a response to a fructose-rich diet (FRD). Materials and methods: Both male and female Wistar rats were given a 10% (w/v) fructose solution for 9 weeks. We measured the biochemical parameters, blood pressure (BP) and heart rate. We used Western blot and real-time polymerase chain reaction (PCR) to quantify protein and gene expression. Results: In the male rats, the FRD elevated BP and expression of cardiac angiotensin-converting enzyme (ACE), while the expression of angiotensin-converting enzyme 2 (ACE2) and angiotensin II Type 2 receptor (AT(2)R) were significantly decreased. In female rats, there were no changes in cardiac RAS expression due to FRD. Furthermore, the ACE/AT(1)R axis was overexpressed in the FRD male rats aortae, while only AT(1)R was upregulated in the FRD female rats aortae. ACE2 expression remained unchanged in the aortae of both genders receiving the FRD. Conclusions: The FRD induced gender-specific changes in the expression of the RAS in the heart and aortae of male rats. Further investigations are required in order to get a comprehensive understanding of the underlying mechanisms of gender-specific fructose-induced cardiovascular pathologies.
T2  - Journal of the Renin-Angiotensin-Aldosterone System
T1  - Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta
VL  - 17
IS  - 2
DO  - 10.1177/1470320316642915
ER  - 

@article{
author = "Bundalo, Maja M. and Živković, Maja and Romić, Snježana Đ. and Tepavčević, Snežana and Korićanac, Goran and Đurić, Tamara and Stanković, Aleksandra",
year = "2016",
abstract = "Introduction: The cardiovascular renin-angiotensin system (RAS) could be affected by gender and dietary regime. We hypothesized that male rats will be more susceptible to activation of RAS in the heart and aorta, as a response to a fructose-rich diet (FRD). Materials and methods: Both male and female Wistar rats were given a 10% (w/v) fructose solution for 9 weeks. We measured the biochemical parameters, blood pressure (BP) and heart rate. We used Western blot and real-time polymerase chain reaction (PCR) to quantify protein and gene expression. Results: In the male rats, the FRD elevated BP and expression of cardiac angiotensin-converting enzyme (ACE), while the expression of angiotensin-converting enzyme 2 (ACE2) and angiotensin II Type 2 receptor (AT(2)R) were significantly decreased. In female rats, there were no changes in cardiac RAS expression due to FRD. Furthermore, the ACE/AT(1)R axis was overexpressed in the FRD male rats aortae, while only AT(1)R was upregulated in the FRD female rats aortae. ACE2 expression remained unchanged in the aortae of both genders receiving the FRD. Conclusions: The FRD induced gender-specific changes in the expression of the RAS in the heart and aortae of male rats. Further investigations are required in order to get a comprehensive understanding of the underlying mechanisms of gender-specific fructose-induced cardiovascular pathologies.",
journal = "Journal of the Renin-Angiotensin-Aldosterone System",
title = "Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta",
volume = "17",
number = "2",
doi = "10.1177/1470320316642915"
}

Bundalo, M. M., Živković, M., Romić, S. Đ., Tepavčević, S., Korićanac, G., Đurić, T.,& Stanković, A.. (2016). Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta. in Journal of the Renin-Angiotensin-Aldosterone System, 17(2).
https://doi.org/10.1177/1470320316642915

Bundalo MM, Živković M, Romić SĐ, Tepavčević S, Korićanac G, Đurić T, Stanković A. Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta. in Journal of the Renin-Angiotensin-Aldosterone System. 2016;17(2).
doi:10.1177/1470320316642915 .

Bundalo, Maja M., Živković, Maja, Romić, Snježana Đ., Tepavčević, Snežana, Korićanac, Goran, Đurić, Tamara, Stanković, Aleksandra, "Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta" in Journal of the Renin-Angiotensin-Aldosterone System, 17, no. 2 (2016),
https://doi.org/10.1177/1470320316642915 . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Korićanac, Goran
AU  - Vujanac, Ivan
AU  - Đorđević, Ana D.
AU  - Pantelić, Marija
AU  - Romić, Snježana Đ.
AU  - Stanimirovic, Zoran
AU  - Kirovski, Danijela
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1211
AB  - We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their body condition score (BCS) as optimal (3.25 LT = BCS LT = 3.5) and high (4.0 LT = BCS LT = 425). Intravenous glucose tolerance test (GTT) and liver biopsies were carried out at day 10 before calving. Blood samples were collected before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels were determined at each sample point. In addition, beta-hydroxybutyrate and triglycerides levels were measured in the basal samples. The liver biopsies were analyzed for total lipid content and protein expression of insulin receptor beta (IR beta), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1). Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of the IR beta, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation. (C) 2016 Elsevier Ltd. All rights reserved.
T2  - Research in Veterinary Science
T1  - Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression
VL  - 107
SP  - 16
EP  - 19
DO  - 10.1016/j.rvsc.2016.04.007
ER  - 

@article{
author = "Prodanović, Radiša and Korićanac, Goran and Vujanac, Ivan and Đorđević, Ana D. and Pantelić, Marija and Romić, Snježana Đ. and Stanimirovic, Zoran and Kirovski, Danijela",
year = "2016",
abstract = "We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their body condition score (BCS) as optimal (3.25 LT = BCS LT = 3.5) and high (4.0 LT = BCS LT = 425). Intravenous glucose tolerance test (GTT) and liver biopsies were carried out at day 10 before calving. Blood samples were collected before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels were determined at each sample point. In addition, beta-hydroxybutyrate and triglycerides levels were measured in the basal samples. The liver biopsies were analyzed for total lipid content and protein expression of insulin receptor beta (IR beta), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1). Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of the IR beta, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation. (C) 2016 Elsevier Ltd. All rights reserved.",
journal = "Research in Veterinary Science",
title = "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression",
volume = "107",
pages = "16-19",
doi = "10.1016/j.rvsc.2016.04.007"
}

Prodanović, R., Korićanac, G., Vujanac, I., Đorđević, A. D., Pantelić, M., Romić, S. Đ., Stanimirovic, Z.,& Kirovski, D.. (2016). Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. in Research in Veterinary Science, 107, 16-19.
https://doi.org/10.1016/j.rvsc.2016.04.007

Prodanović R, Korićanac G, Vujanac I, Đorđević AD, Pantelić M, Romić SĐ, Stanimirovic Z, Kirovski D. Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. in Research in Veterinary Science. 2016;107:16-19.
doi:10.1016/j.rvsc.2016.04.007 .

Prodanović, Radiša, Korićanac, Goran, Vujanac, Ivan, Đorđević, Ana D., Pantelić, Marija, Romić, Snježana Đ., Stanimirovic, Zoran, Kirovski, Danijela, "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression" in Research in Veterinary Science, 107 (2016):16-19,
https://doi.org/10.1016/j.rvsc.2016.04.007 . .

TY  - JOUR
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
AU  - Ćulafić, Tijana
AU  - Romić, Snježana Đ.
AU  - Stojiljković, Mojca D.
AU  - Kostić, Milan
AU  - Pantelić, Marija
AU  - Tepavčević, Snežana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/913
AB  - Increase in fructose consumption together with decrease in physical activity contributes to the development of metabolic syndrome and consequently cardiovascular diseases. The current study examined the preventive role of exercise on defects in cardiac insulin signaling and function of endothelial nitric oxide synthase (eNOS) in fructose fed rats. Male Wistar rats were divided into control, sedentary fructose (received 10% fructose for 9 weeks) and exercise fructose (additionally exposed to low intensity exercise) groups. Concentration of triglycerides, glucose, insulin and visceral adipose tissue weight were determined to estimate metabolic syndrome development. Expression and/or phosphorylation of cardiac insulin receptor (IR), insulin receptor substrate 1 (IRS1), tyrosine-specific protein phosphatase 1B (PTP1B), Akt, extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and eNOS were evaluated. Fructose overload increased visceral adipose tissue, insulin concentration and homeostasis model assessment index. Exercise managed to decrease visceral adiposity and insulin level and to increase insulin sensitivity. Fructose diet increased level of cardiac PTP1B and pIRS1 (Ser307), while levels of IR and ERK1/2, as well as pIRS1 (Tyr 632), pAkt (Ser473, Thr308) and pERK1/2 were decreased. These disturbances were accompanied by reduced phosphorylation of eNOS at Ser1177. Exercise managed to prevent most of the disturbances in insulin signaling caused by fructose diet (except phosphorylation of IRS1 at Tyr 632 and phosphorylation and protein expression of ERK1/2) and consequently restored function of eNOS. Low intensity exercise could be considered as efficient treatment of cardiac insulin resistance induced by fructose diet. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
PB  - Elsevier
T2  - Molecular and Cellular Endocrinology
T1  - Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet
VL  - 420
IS  - C
SP  - 97
EP  - 104
DO  - 10.1016/j.mce.2015.11.032
ER  - 

@article{
author = "Stanišić, Jelena and Korićanac, Goran and Ćulafić, Tijana and Romić, Snježana Đ. and Stojiljković, Mojca D. and Kostić, Milan and Pantelić, Marija and Tepavčević, Snežana",
year = "2016",
abstract = "Increase in fructose consumption together with decrease in physical activity contributes to the development of metabolic syndrome and consequently cardiovascular diseases. The current study examined the preventive role of exercise on defects in cardiac insulin signaling and function of endothelial nitric oxide synthase (eNOS) in fructose fed rats. Male Wistar rats were divided into control, sedentary fructose (received 10% fructose for 9 weeks) and exercise fructose (additionally exposed to low intensity exercise) groups. Concentration of triglycerides, glucose, insulin and visceral adipose tissue weight were determined to estimate metabolic syndrome development. Expression and/or phosphorylation of cardiac insulin receptor (IR), insulin receptor substrate 1 (IRS1), tyrosine-specific protein phosphatase 1B (PTP1B), Akt, extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and eNOS were evaluated. Fructose overload increased visceral adipose tissue, insulin concentration and homeostasis model assessment index. Exercise managed to decrease visceral adiposity and insulin level and to increase insulin sensitivity. Fructose diet increased level of cardiac PTP1B and pIRS1 (Ser307), while levels of IR and ERK1/2, as well as pIRS1 (Tyr 632), pAkt (Ser473, Thr308) and pERK1/2 were decreased. These disturbances were accompanied by reduced phosphorylation of eNOS at Ser1177. Exercise managed to prevent most of the disturbances in insulin signaling caused by fructose diet (except phosphorylation of IRS1 at Tyr 632 and phosphorylation and protein expression of ERK1/2) and consequently restored function of eNOS. Low intensity exercise could be considered as efficient treatment of cardiac insulin resistance induced by fructose diet. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Molecular and Cellular Endocrinology",
title = "Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet",
volume = "420",
number = "C",
pages = "97-104",
doi = "10.1016/j.mce.2015.11.032"
}

Stanišić, J., Korićanac, G., Ćulafić, T., Romić, S. Đ., Stojiljković, M. D., Kostić, M., Pantelić, M.,& Tepavčević, S.. (2016). Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet. in Molecular and Cellular Endocrinology
Elsevier., 420(C), 97-104.
https://doi.org/10.1016/j.mce.2015.11.032

Stanišić J, Korićanac G, Ćulafić T, Romić SĐ, Stojiljković MD, Kostić M, Pantelić M, Tepavčević S. Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet. in Molecular and Cellular Endocrinology. 2016;420(C):97-104.
doi:10.1016/j.mce.2015.11.032 .

Stanišić, Jelena, Korićanac, Goran, Ćulafić, Tijana, Romić, Snježana Đ., Stojiljković, Mojca D., Kostić, Milan, Pantelić, Marija, Tepavčević, Snežana, "Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet" in Molecular and Cellular Endocrinology, 420, no. C (2016):97-104,
https://doi.org/10.1016/j.mce.2015.11.032 . .

TY  - THES
AU  - Romić, Snježana Đ.
PY  - 2015
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=2310
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:10216/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024953522
UR  - http://nardus.mpn.gov.rs/123456789/4170
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7272
AB  - Fruktoza je prirodni šećer, ĉiji se unos poslednjih decenija dramatiĉno povećao, najvećim delom zbog povećane upotrebe kukuruznog sirupa sa visokim sadrţajem fruktoze. Povećan unos fruktoze vodi ka razvoju metaboliĉkog sindroma kod ljudi i eksperimentalnih ţivotinja. Pored promena u jetri, adipoznom tkivu i skeletnim mišićima, ishrana bogata fruktozom je praćena i razvojem insulinske rezistencije u srcu. Poznato je da insulin ostvaruje svoje efekte na ćelije srca i da u stanju insulinske rezistencije u srcu dolazi do poremećaja u signalnom putu Akt/eNOS i balansu korišćenja energetskih supstrata. Posledice povećanog unosa fruktoze su polno zavisne, pri ĉemu je protektivni efekat pripisan estrogenim hormonima. Fiziološke koncentracije estrogena poboljšavaju osetljivost na insulin i deluju kardioprotektivno. TakoĊe, estradiol utiĉe na specifiĉno delovanje insulina u srcu.Cilj ove studije bio je analiza efekta estradiola na procese u srcu regulisane insulinom u stanju insulinske rezistencije izazvane ishranom bogatom fruktozom. Ţenke pacova su podvrgnute standardnoj ishrani ili ishrani obogaćenoj 10% rastvorom fruktoze tokom 9 nedelja i ovarijektomisane 2 nedelje pre ţrtvovanja, pri ĉemu je polovina ţivotinja na fruktoznoj ishrani dan nakon ovarijektomije podvrgnuta tretmanu estradiolom. U cilju izuĉavanja efekata ishrane bogate fruktozom i estradiola na insulinom regulisanu fosforilaciju i unutarćelijsku lokalizaciju ispitivanih molekula, polovina ţivotinja je tretirana insulinom 40 minuta pre ţrtvovanja. Analizirani su molekuli iz insulinskog signalnog puta (IRS-1, Akt i ERK1/2), kao i efektorni molekuli ĉiju funkciju reguliše insulin, kao što su eNOS, Na+/K+-ATP-aza i molekuli ukljuĉeni u transport i metabolizam energetskih supstrata u srcu (GLUT1, GLUT4, CD36, lipin 1 i CPTI) i lipidni profil srca. Ispitivana je ekspresija ovih molekula na nivou proteina, kao i njihova fosforilacija ili unutarćelijska lokalizacija.Ishrana bogata fruktozom je povećala unos teĉnosti i smanjila unos hrane, dok je povećala ukupan kalorijski unos i koncentraciju leptina u plazmi. Estradiol je većim delom poništio štetne efekte ishrane na regulaciju apetita, verovatno preko povećanjacentralne osetljivosti na leptin. Ţivotinje na fruktoznoj ishrani su imale povišene koncentracije triglicerida i insulina u plazmi i povećan HOMA indeks, smanjenu koncentraciju slobodnih masnih kiselina, dok je koncentracija glukoze bila nepromenjena...
AB  - Fructose is natural sugar whose intake has increased dramatically over the past decades, mostly due to increased consumption of high-fructose corn syrup. Increased intake of fructose initiates development of metabolic syndrome phenotype in humans and experimental animals. In addition to changes in liver, adippose tissue and skeletal muscle, fructose-rich diеt is accompanied by cardiac insulin resistance. Cardiac muscle is a target of insulin. Insulin resistance is accompanied by disturbances in Akt/eNOS signalling and altered cardiac usage of energetic substrates. Consequences of enhanced fructose intake are shown to be sex-dependent and the protective effect is attributed to estrogens. Physiological concentrations of estrogens improve insulin sensitivity and they are cardioprotective. Also, estrogens affect specific cardiac insulin action.The aim of the present study was to analyze effects of estradiol on insulin-regulated processes in the heart in insulin resistance state. Female rats were subjected to standard diet or diet containing 10% fructose in drinking water during 9 weeks. Two weeks before sacrifice, all animals were bilaterally ovariectomized and half of the fructose-fed animals were subjected to estradiol replacement treatment, day after ovariectomy. In order to study fructose-rich diet and estradiol effects on the insulin regulated phosphorylations and subcellular localization of analyzed molecules, half of the animals were treated with insulin, 40 min before killing. Insulin signaling molecules (IRS-1, Akt i ERK1/2) were analyzed, as well as insulin regulated effector molecules, such as eNOS, Na+/K+-ATPase and molecules involved in the transport and metabolism of energetic substrates in the heart (GLUT1, GLUT4, CD36, lipin 1 i CPTI), as well as profile of cardiac lipids. We examined the protein exppression of the molecules, as well as their phosphorylation or subcellular localization.Fructose-rich diet increased liquid intake and decreased food intake, while it increased total caloric intake and plasma leptin concentration. Estradiol abolished most of the detrimental effects of diet on appetite regulation, probably through increase in central leptin sensitivity. The fructose-fed rats had increased plasma triglycerides andinsulin levels and increased HOMA index, decreased plasma free fatty acid level, while glucose concentration was unaltered...
PB  - Универзитет у Београду, Биолошки факултет
T2  - Универзитет у Београду
T1  - Efekti estradiola u srcu pacova sa insulinskom rezistencijom izazvanom ishranom bogatom fruktozom
T1  - Effects of estradiol in the heart of rats with fructose-rich diet-induced insulin resistance
UR  - https://hdl.handle.net/21.15107/rcub_nardus_4170
ER  - 

@phdthesis{
author = "Romić, Snježana Đ.",
year = "2015",
abstract = "Fruktoza je prirodni šećer, ĉiji se unos poslednjih decenija dramatiĉno povećao, najvećim delom zbog povećane upotrebe kukuruznog sirupa sa visokim sadrţajem fruktoze. Povećan unos fruktoze vodi ka razvoju metaboliĉkog sindroma kod ljudi i eksperimentalnih ţivotinja. Pored promena u jetri, adipoznom tkivu i skeletnim mišićima, ishrana bogata fruktozom je praćena i razvojem insulinske rezistencije u srcu. Poznato je da insulin ostvaruje svoje efekte na ćelije srca i da u stanju insulinske rezistencije u srcu dolazi do poremećaja u signalnom putu Akt/eNOS i balansu korišćenja energetskih supstrata. Posledice povećanog unosa fruktoze su polno zavisne, pri ĉemu je protektivni efekat pripisan estrogenim hormonima. Fiziološke koncentracije estrogena poboljšavaju osetljivost na insulin i deluju kardioprotektivno. TakoĊe, estradiol utiĉe na specifiĉno delovanje insulina u srcu.Cilj ove studije bio je analiza efekta estradiola na procese u srcu regulisane insulinom u stanju insulinske rezistencije izazvane ishranom bogatom fruktozom. Ţenke pacova su podvrgnute standardnoj ishrani ili ishrani obogaćenoj 10% rastvorom fruktoze tokom 9 nedelja i ovarijektomisane 2 nedelje pre ţrtvovanja, pri ĉemu je polovina ţivotinja na fruktoznoj ishrani dan nakon ovarijektomije podvrgnuta tretmanu estradiolom. U cilju izuĉavanja efekata ishrane bogate fruktozom i estradiola na insulinom regulisanu fosforilaciju i unutarćelijsku lokalizaciju ispitivanih molekula, polovina ţivotinja je tretirana insulinom 40 minuta pre ţrtvovanja. Analizirani su molekuli iz insulinskog signalnog puta (IRS-1, Akt i ERK1/2), kao i efektorni molekuli ĉiju funkciju reguliše insulin, kao što su eNOS, Na+/K+-ATP-aza i molekuli ukljuĉeni u transport i metabolizam energetskih supstrata u srcu (GLUT1, GLUT4, CD36, lipin 1 i CPTI) i lipidni profil srca. Ispitivana je ekspresija ovih molekula na nivou proteina, kao i njihova fosforilacija ili unutarćelijska lokalizacija.Ishrana bogata fruktozom je povećala unos teĉnosti i smanjila unos hrane, dok je povećala ukupan kalorijski unos i koncentraciju leptina u plazmi. Estradiol je većim delom poništio štetne efekte ishrane na regulaciju apetita, verovatno preko povećanjacentralne osetljivosti na leptin. Ţivotinje na fruktoznoj ishrani su imale povišene koncentracije triglicerida i insulina u plazmi i povećan HOMA indeks, smanjenu koncentraciju slobodnih masnih kiselina, dok je koncentracija glukoze bila nepromenjena..., Fructose is natural sugar whose intake has increased dramatically over the past decades, mostly due to increased consumption of high-fructose corn syrup. Increased intake of fructose initiates development of metabolic syndrome phenotype in humans and experimental animals. In addition to changes in liver, adippose tissue and skeletal muscle, fructose-rich diеt is accompanied by cardiac insulin resistance. Cardiac muscle is a target of insulin. Insulin resistance is accompanied by disturbances in Akt/eNOS signalling and altered cardiac usage of energetic substrates. Consequences of enhanced fructose intake are shown to be sex-dependent and the protective effect is attributed to estrogens. Physiological concentrations of estrogens improve insulin sensitivity and they are cardioprotective. Also, estrogens affect specific cardiac insulin action.The aim of the present study was to analyze effects of estradiol on insulin-regulated processes in the heart in insulin resistance state. Female rats were subjected to standard diet or diet containing 10% fructose in drinking water during 9 weeks. Two weeks before sacrifice, all animals were bilaterally ovariectomized and half of the fructose-fed animals were subjected to estradiol replacement treatment, day after ovariectomy. In order to study fructose-rich diet and estradiol effects on the insulin regulated phosphorylations and subcellular localization of analyzed molecules, half of the animals were treated with insulin, 40 min before killing. Insulin signaling molecules (IRS-1, Akt i ERK1/2) were analyzed, as well as insulin regulated effector molecules, such as eNOS, Na+/K+-ATPase and molecules involved in the transport and metabolism of energetic substrates in the heart (GLUT1, GLUT4, CD36, lipin 1 i CPTI), as well as profile of cardiac lipids. We examined the protein exppression of the molecules, as well as their phosphorylation or subcellular localization.Fructose-rich diet increased liquid intake and decreased food intake, while it increased total caloric intake and plasma leptin concentration. Estradiol abolished most of the detrimental effects of diet on appetite regulation, probably through increase in central leptin sensitivity. The fructose-fed rats had increased plasma triglycerides andinsulin levels and increased HOMA index, decreased plasma free fatty acid level, while glucose concentration was unaltered...",
publisher = "Универзитет у Београду, Биолошки факултет",
journal = "Универзитет у Београду",
title = "Efekti estradiola u srcu pacova sa insulinskom rezistencijom izazvanom ishranom bogatom fruktozom, Effects of estradiol in the heart of rats with fructose-rich diet-induced insulin resistance",
url = "https://hdl.handle.net/21.15107/rcub_nardus_4170"
}

Romić, S. Đ.. (2015). Efekti estradiola u srcu pacova sa insulinskom rezistencijom izazvanom ishranom bogatom fruktozom. in Универзитет у Београду
Универзитет у Београду, Биолошки факултет..
https://hdl.handle.net/21.15107/rcub_nardus_4170

Romić SĐ. Efekti estradiola u srcu pacova sa insulinskom rezistencijom izazvanom ishranom bogatom fruktozom. in Универзитет у Београду. 2015;.
https://hdl.handle.net/21.15107/rcub_nardus_4170 .

Romić, Snježana Đ., "Efekti estradiola u srcu pacova sa insulinskom rezistencijom izazvanom ishranom bogatom fruktozom" in Универзитет у Београду (2015),
https://hdl.handle.net/21.15107/rcub_nardus_4170 .

TY  - JOUR
AU  - Romić, Snježana Đ.
AU  - Tepavčević, Snežana
AU  - Žakula, Zorica
AU  - Milosavljević, Tijana
AU  - Kostić, Milan
AU  - Petković, Marijana
AU  - Korićanac, Goran
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6053
AB  - To give new insight to alterations of cardiac lipid metabolism accompanied by a fructose-rich diet (FRD), rats of both sexes were exposed to 10 % fructose in drinking water during 9 weeks. The protein level and subcellular localization of the main regulators of cardiac lipid metabolism, such as lipin 1, peroxisome proliferator-activated receptor alpha (PPAR alpha), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1 alpha), carnitine palmitoyltransferase I (CPTI), and CD36 were studied. Caloric intake in fructose-fed rats (FFR) of both sexes was increased. Circulating triacylglyceroles (TAG) and non-esterified fatty acids were increased in male FFR, while females increased visceral adiposity and blood TAG. Total expression of lipin 1 in cardiac cell lysate and its cytosolic and microsomal level were increased in the hearts of male FFR. PPAR alpha and PGC-1 alpha content were decreased in the nuclear extract. In addition, cardiac deposition of TAG in male FFR was elevated, as well as inhibitory phosphorylation of insulin receptor substrate 1 (IRS-1). In contrast, in female FFR, lipin 1 level was increased in nuclear extract only, while overall CPTI expression and phosphorylation of IRS-1 at serine 307 were decreased. The results of our study suggest that fructose diet causes gender-dependent alterations in cardiac lipid metabolism. Potentially detrimental effects of FRD seem to be limited to male rats. Most of the observed changes might be a consequence of elevated expression and altered localization of lipin 1. Increased inhibitory phosphorylation of IRS-1 is possible link between cardiac lipid metabolism and insulin resistance in FFR.
T2  - Lipids
T1  - Gender Differences in the Expression and Cellular Localization of Lipin 1 in the Hearts of Fructose-Fed Rats
VL  - 49
IS  - 7
SP  - 655
EP  - 663
DO  - 10.1007/s11745-014-3909-4
ER  - 

@article{
author = "Romić, Snježana Đ. and Tepavčević, Snežana and Žakula, Zorica and Milosavljević, Tijana and Kostić, Milan and Petković, Marijana and Korićanac, Goran",
year = "2014",
abstract = "To give new insight to alterations of cardiac lipid metabolism accompanied by a fructose-rich diet (FRD), rats of both sexes were exposed to 10 % fructose in drinking water during 9 weeks. The protein level and subcellular localization of the main regulators of cardiac lipid metabolism, such as lipin 1, peroxisome proliferator-activated receptor alpha (PPAR alpha), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1 alpha), carnitine palmitoyltransferase I (CPTI), and CD36 were studied. Caloric intake in fructose-fed rats (FFR) of both sexes was increased. Circulating triacylglyceroles (TAG) and non-esterified fatty acids were increased in male FFR, while females increased visceral adiposity and blood TAG. Total expression of lipin 1 in cardiac cell lysate and its cytosolic and microsomal level were increased in the hearts of male FFR. PPAR alpha and PGC-1 alpha content were decreased in the nuclear extract. In addition, cardiac deposition of TAG in male FFR was elevated, as well as inhibitory phosphorylation of insulin receptor substrate 1 (IRS-1). In contrast, in female FFR, lipin 1 level was increased in nuclear extract only, while overall CPTI expression and phosphorylation of IRS-1 at serine 307 were decreased. The results of our study suggest that fructose diet causes gender-dependent alterations in cardiac lipid metabolism. Potentially detrimental effects of FRD seem to be limited to male rats. Most of the observed changes might be a consequence of elevated expression and altered localization of lipin 1. Increased inhibitory phosphorylation of IRS-1 is possible link between cardiac lipid metabolism and insulin resistance in FFR.",
journal = "Lipids",
title = "Gender Differences in the Expression and Cellular Localization of Lipin 1 in the Hearts of Fructose-Fed Rats",
volume = "49",
number = "7",
pages = "655-663",
doi = "10.1007/s11745-014-3909-4"
}

Romić, S. Đ., Tepavčević, S., Žakula, Z., Milosavljević, T., Kostić, M., Petković, M.,& Korićanac, G.. (2014). Gender Differences in the Expression and Cellular Localization of Lipin 1 in the Hearts of Fructose-Fed Rats. in Lipids, 49(7), 655-663.
https://doi.org/10.1007/s11745-014-3909-4

Romić SĐ, Tepavčević S, Žakula Z, Milosavljević T, Kostić M, Petković M, Korićanac G. Gender Differences in the Expression and Cellular Localization of Lipin 1 in the Hearts of Fructose-Fed Rats. in Lipids. 2014;49(7):655-663.
doi:10.1007/s11745-014-3909-4 .

Romić, Snježana Đ., Tepavčević, Snežana, Žakula, Zorica, Milosavljević, Tijana, Kostić, Milan, Petković, Marijana, Korićanac, Goran, "Gender Differences in the Expression and Cellular Localization of Lipin 1 in the Hearts of Fructose-Fed Rats" in Lipids, 49, no. 7 (2014):655-663,
https://doi.org/10.1007/s11745-014-3909-4 . .

TY  - JOUR
AU  - Tepavčević, Snežana
AU  - Milutinovic, Danijela Vojnovic
AU  - Macut, Djuro
AU  - Žakula, Zorica
AU  - Nikolić, Marina
AU  - Bozic-Antic, Ivana
AU  - Romić, Snježana Đ.
AU  - Bjekic-Macut, Jelica
AU  - Matić, Gordana
AU  - Korićanac, Goran
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5970
AB  - It is supposed that women with polycystic ovary syndrome (PCOS) are prone to develop cardiovascular disease as a consequence of multiple risk factors that are mostly related to the state of insulin resistance and consequent hyperinsulinemia. In the present study, we evaluated insulin signaling and glucose transporters (GLUT) in cardiac cells of dihydrotestosterone (DHT) treated female rats as an animal model of PCOS. Expression of proteins involved in cardiac insulin signaling pathways and glucose transporters, as well as their phosphorylation or intracellular localization were studied by Western blot analysis in DHT-treated and control rats. Treatment with DHT resulted in increased body mass, absolute mass of the heart, elevated plasma insulin concentration, dyslipidemia and insulin resistance. At the molecular level, DHT treatment did not change protein expression of cardiac insulin receptor and insulin receptor substrate 1, while phosphorylation of the substrate at serine 307 was increased. Unexpectedly, although expression of downstream Akt kinase and its phosphorylation at threonine 308 were not altered, phosphoiylation of Akt at serine 473 was increased in the heart of DHT-treated rats. In contrast, expression and phosphorylation of extracellular signal regulated kinases 1/2 were decreased. Plasma membrane contents of GLUT1 and GLUT4 were decreased, as well as the expression of GLUT4 in cardiac cells at the end of androgen treatment. The obtained results provide evidence for alterations in expression and especially in functional characteristics of insulin signaling molecules and glucose transporters in the heart of DHT-treated rats with PCOS, indicating impaired cardiac insulin action. (c) 2014 Elsevier Ltd. All rights reserved.
T2  - Journal of Steroid Biochemistry and Molecular Biology
T1  - Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome
VL  - 141
SP  - 71
EP  - 76
DO  - 10.1016/j.jsbmb.2014.01.006
ER  - 

@article{
author = "Tepavčević, Snežana and Milutinovic, Danijela Vojnovic and Macut, Djuro and Žakula, Zorica and Nikolić, Marina and Bozic-Antic, Ivana and Romić, Snježana Đ. and Bjekic-Macut, Jelica and Matić, Gordana and Korićanac, Goran",
year = "2014",
abstract = "It is supposed that women with polycystic ovary syndrome (PCOS) are prone to develop cardiovascular disease as a consequence of multiple risk factors that are mostly related to the state of insulin resistance and consequent hyperinsulinemia. In the present study, we evaluated insulin signaling and glucose transporters (GLUT) in cardiac cells of dihydrotestosterone (DHT) treated female rats as an animal model of PCOS. Expression of proteins involved in cardiac insulin signaling pathways and glucose transporters, as well as their phosphorylation or intracellular localization were studied by Western blot analysis in DHT-treated and control rats. Treatment with DHT resulted in increased body mass, absolute mass of the heart, elevated plasma insulin concentration, dyslipidemia and insulin resistance. At the molecular level, DHT treatment did not change protein expression of cardiac insulin receptor and insulin receptor substrate 1, while phosphorylation of the substrate at serine 307 was increased. Unexpectedly, although expression of downstream Akt kinase and its phosphorylation at threonine 308 were not altered, phosphoiylation of Akt at serine 473 was increased in the heart of DHT-treated rats. In contrast, expression and phosphorylation of extracellular signal regulated kinases 1/2 were decreased. Plasma membrane contents of GLUT1 and GLUT4 were decreased, as well as the expression of GLUT4 in cardiac cells at the end of androgen treatment. The obtained results provide evidence for alterations in expression and especially in functional characteristics of insulin signaling molecules and glucose transporters in the heart of DHT-treated rats with PCOS, indicating impaired cardiac insulin action. (c) 2014 Elsevier Ltd. All rights reserved.",
journal = "Journal of Steroid Biochemistry and Molecular Biology",
title = "Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome",
volume = "141",
pages = "71-76",
doi = "10.1016/j.jsbmb.2014.01.006"
}

Tepavčević, S., Milutinovic, D. V., Macut, D., Žakula, Z., Nikolić, M., Bozic-Antic, I., Romić, S. Đ., Bjekic-Macut, J., Matić, G.,& Korićanac, G.. (2014). Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome. in Journal of Steroid Biochemistry and Molecular Biology, 141, 71-76.
https://doi.org/10.1016/j.jsbmb.2014.01.006

Tepavčević S, Milutinovic DV, Macut D, Žakula Z, Nikolić M, Bozic-Antic I, Romić SĐ, Bjekic-Macut J, Matić G, Korićanac G. Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome. in Journal of Steroid Biochemistry and Molecular Biology. 2014;141:71-76.
doi:10.1016/j.jsbmb.2014.01.006 .

Tepavčević, Snežana, Milutinovic, Danijela Vojnovic, Macut, Djuro, Žakula, Zorica, Nikolić, Marina, Bozic-Antic, Ivana, Romić, Snježana Đ., Bjekic-Macut, Jelica, Matić, Gordana, Korićanac, Goran, "Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome" in Journal of Steroid Biochemistry and Molecular Biology, 141 (2014):71-76,
https://doi.org/10.1016/j.jsbmb.2014.01.006 . .

TY  - JOUR
AU  - Korićanac, Goran
AU  - Tepavčević, Snežana
AU  - Romić, Snježana Đ.
AU  - Milosavljević, Tijana
AU  - Stojiljković, Mojca D.
AU  - Žakula, Zorica
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5879
T2  - Hormone and Metabolic Research
T1  - Expression and Cellular Distribution of Glucose Transporters and Alpha Subunits of Na+/K+-ATPase in the Heart of Fructose-fed Female Rats: The Role of Estradiol
VL  - 46
IS  - 2
SP  - 109
EP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5879
ER  - 

@article{
author = "Korićanac, Goran and Tepavčević, Snežana and Romić, Snježana Đ. and Milosavljević, Tijana and Stojiljković, Mojca D. and Žakula, Zorica",
year = "2014",
journal = "Hormone and Metabolic Research",
title = "Expression and Cellular Distribution of Glucose Transporters and Alpha Subunits of Na+/K+-ATPase in the Heart of Fructose-fed Female Rats: The Role of Estradiol",
volume = "46",
number = "2",
pages = "109-115",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5879"
}

Korićanac, G., Tepavčević, S., Romić, S. Đ., Milosavljević, T., Stojiljković, M. D.,& Žakula, Z.. (2014). Expression and Cellular Distribution of Glucose Transporters and Alpha Subunits of Na+/K+-ATPase in the Heart of Fructose-fed Female Rats: The Role of Estradiol. in Hormone and Metabolic Research, 46(2), 109-115.
https://hdl.handle.net/21.15107/rcub_vinar_5879

Korićanac G, Tepavčević S, Romić SĐ, Milosavljević T, Stojiljković MD, Žakula Z. Expression and Cellular Distribution of Glucose Transporters and Alpha Subunits of Na+/K+-ATPase in the Heart of Fructose-fed Female Rats: The Role of Estradiol. in Hormone and Metabolic Research. 2014;46(2):109-115.
https://hdl.handle.net/21.15107/rcub_vinar_5879 .

Korićanac, Goran, Tepavčević, Snežana, Romić, Snježana Đ., Milosavljević, Tijana, Stojiljković, Mojca D., Žakula, Zorica, "Expression and Cellular Distribution of Glucose Transporters and Alpha Subunits of Na+/K+-ATPase in the Heart of Fructose-fed Female Rats: The Role of Estradiol" in Hormone and Metabolic Research, 46, no. 2 (2014):109-115,
https://hdl.handle.net/21.15107/rcub_vinar_5879 .

TY  - CONF
AU  - Bundalo, Maja M.
AU  - Tepavčević, Snežana
AU  - Romić, Snježana Đ.
AU  - Korićanac, Goran
AU  - Živković, Maja
AU  - Stanković, Aleksandra
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5741
AB  - Background: Heart disease or diabetes could be promoted in people who consume sugar. High dietary
intake of fructose also increases risk for heart disease. The effects of fructose diet on metabolism, lipid
profile and blood pressure are dependent of sex. Renin-angiotensin system (RAS) impairs insulin sensi-
tivity, whereas hyperinsulinemia and insulin resistance promotes the development of cardiovascular dis-
orders. We examined the effects of fructose rich diet (FRD) and estradiol (E2) on the expression of ACE,
ACE2, AT1R and AT2R in the rat heart.
Methods and Results: 21 day old female rats were divided in control group and FRD group. At two
weeks before sacrifice, animals were ovariectomised and half of the FRD rats were subjected to E2 re-
placement. FRD increased ACE (p<0.001) and AT1R (p<0.05) protein expression, while AT2R expression
was decreased (p<0.05). ACE2 protein expression was unaltered. mRNA expression for AT1R was unal-
tered, as assessed by qPCR. E2 treatment significantly decreased protein expression for ACE (p<0.001)
and AT1R (p<0.01), while ACE2 (p<0.01) and AT2R (p<0.01) expression was increased. Effect of E2 treat-
ment on AT1R gene expression was not statistically significant but showed trend toward lowering AT1R
gene expression. We didn’t detected AT2R gene expression.
Conclusion: FRD affects almost all components of the RAS in the rat heart.The increase of the ACE and
AT1R protein expression and decrease of AT2R expression could be the the way that leads to the devel-
opment of cardiovascular disorders. The E2 shows strong protective effect in FRD through reversion of the
changes in RAS components expression.
C3  - Cardiology
T1  - The expression of ACE, ACE2, AT1 and AT2 receptors in the heart of fructose fed ovariectomized rats-Effect of estradiol
VL  - 126
IS  - Suppl. 2
SP  - 353
EP  - 353
DO  - 10.1159/000355890
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5741
ER  - 

@conference{
author = "Bundalo, Maja M. and Tepavčević, Snežana and Romić, Snježana Đ. and Korićanac, Goran and Živković, Maja and Stanković, Aleksandra",
year = "2013",
abstract = "Background: Heart disease or diabetes could be promoted in people who consume sugar. High dietary
intake of fructose also increases risk for heart disease. The effects of fructose diet on metabolism, lipid
profile and blood pressure are dependent of sex. Renin-angiotensin system (RAS) impairs insulin sensi-
tivity, whereas hyperinsulinemia and insulin resistance promotes the development of cardiovascular dis-
orders. We examined the effects of fructose rich diet (FRD) and estradiol (E2) on the expression of ACE,
ACE2, AT1R and AT2R in the rat heart.
Methods and Results: 21 day old female rats were divided in control group and FRD group. At two
weeks before sacrifice, animals were ovariectomised and half of the FRD rats were subjected to E2 re-
placement. FRD increased ACE (p<0.001) and AT1R (p<0.05) protein expression, while AT2R expression
was decreased (p<0.05). ACE2 protein expression was unaltered. mRNA expression for AT1R was unal-
tered, as assessed by qPCR. E2 treatment significantly decreased protein expression for ACE (p<0.001)
and AT1R (p<0.01), while ACE2 (p<0.01) and AT2R (p<0.01) expression was increased. Effect of E2 treat-
ment on AT1R gene expression was not statistically significant but showed trend toward lowering AT1R
gene expression. We didn’t detected AT2R gene expression.
Conclusion: FRD affects almost all components of the RAS in the rat heart.The increase of the ACE and
AT1R protein expression and decrease of AT2R expression could be the the way that leads to the devel-
opment of cardiovascular disorders. The E2 shows strong protective effect in FRD through reversion of the
changes in RAS components expression.",
journal = "Cardiology",
title = "The expression of ACE, ACE2, AT1 and AT2 receptors in the heart of fructose fed ovariectomized rats-Effect of estradiol",
volume = "126",
number = "Suppl. 2",
pages = "353-353",
doi = "10.1159/000355890",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5741"
}

Bundalo, M. M., Tepavčević, S., Romić, S. Đ., Korićanac, G., Živković, M.,& Stanković, A.. (2013). The expression of ACE, ACE2, AT1 and AT2 receptors in the heart of fructose fed ovariectomized rats-Effect of estradiol. in Cardiology, 126(Suppl. 2), 353-353.
https://doi.org/10.1159/000355890
https://hdl.handle.net/21.15107/rcub_vinar_5741

Bundalo MM, Tepavčević S, Romić SĐ, Korićanac G, Živković M, Stanković A. The expression of ACE, ACE2, AT1 and AT2 receptors in the heart of fructose fed ovariectomized rats-Effect of estradiol. in Cardiology. 2013;126(Suppl. 2):353-353.
doi:10.1159/000355890
https://hdl.handle.net/21.15107/rcub_vinar_5741 .

Bundalo, Maja M., Tepavčević, Snežana, Romić, Snježana Đ., Korićanac, Goran, Živković, Maja, Stanković, Aleksandra, "The expression of ACE, ACE2, AT1 and AT2 receptors in the heart of fructose fed ovariectomized rats-Effect of estradiol" in Cardiology, 126, no. Suppl. 2 (2013):353-353,
https://doi.org/10.1159/000355890 .,
https://hdl.handle.net/21.15107/rcub_vinar_5741 .