TY  - JOUR
AU  - Segura, Rodrigo
AU  - Vásquez, Gustavo
AU  - Colson, Emmanuel
AU  - Gerbaux, Pascal
AU  - Frischmon, Caroline
AU  - Nešić, Aleksandra
AU  - García, Danni E.
AU  - Cabrera‐Barjas, Gustavo
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9069
AB  - BACKGROUND: Quinoa (Chenopodium quinoa Willd) is an Andean original pseudocereal with high nutritional value. During quinoa processing, large amounts of saponin-rich husks byproducts are obtained. Quinoa saponins, which are biologically active, could be used for various agriculture purposes. Silver nanoparticles have increasingly attracted attention for the management of crop diseases in agriculture. In this work, silver nanoparticles are synthesized by a sustainable and green method, using quinoa husk saponin extract (QE) to evaluate their potential for application in agriculture as biostimulants. RESULTS: Quinoa extract was obtained and characterized by liquid chromatography with tandem mass spectrometry (LC–MS/MS). Sixteen saponin congeners were successfully identified and quantified. The QE obtained was used as a reducing agent for silver ions to synthesize silver nanoparticles (QEAgNPs) under mild conditions. The morphology, particle size, and stability of Ag nanoparticles were investigated by transmission electron microscopy (TEM), ultraviolet–visible spectroscopy (UV-visible), energy-dispersive X-ray (EDS), zeta potential, and Fourier transform infrared spectroscopy with attenuated total reflection (FTIR-ATR). Ultraviolet–visible spectroscopy measurements confirmed the formation of silver nanoparticles in the presence of QE, with estimated particle sizes in a range between 5 and 50 nm. According to the zeta potential values, highly stable nanoparticles were formed. The QE and QEAgNPs (200–1000 μg/mL) were also tested in radish seed bioassay to evaluate their phytotoxicity. The seed germination assays revealed that QEAgNPs possessed a phytostimulant effect on radish seeds in a dose-dependent manner, and no phytotoxicity was observed for both QE and QEAgNPs. CONCLUSION: Silver nanoparticles obtained by a so-called ‘green’ method could be considered as good candidates for application in the agricultural sector for seed treatment, or as foliar sprays and plant-growth-promoters.
T2  - Journal of the Science of Food and Agriculture
T1  - Phytostimulant properties of highly stable silver nanoparticles obtained with saponin extract from Chenopodium quinoa
VL  - 100
IS  - 13
SP  - 4987
EP  - 4994
DO  - 10.1002/jsfa.10529
ER  - 

@article{
author = "Segura, Rodrigo and Vásquez, Gustavo and Colson, Emmanuel and Gerbaux, Pascal and Frischmon, Caroline and Nešić, Aleksandra and García, Danni E. and Cabrera‐Barjas, Gustavo",
year = "2020",
abstract = "BACKGROUND: Quinoa (Chenopodium quinoa Willd) is an Andean original pseudocereal with high nutritional value. During quinoa processing, large amounts of saponin-rich husks byproducts are obtained. Quinoa saponins, which are biologically active, could be used for various agriculture purposes. Silver nanoparticles have increasingly attracted attention for the management of crop diseases in agriculture. In this work, silver nanoparticles are synthesized by a sustainable and green method, using quinoa husk saponin extract (QE) to evaluate their potential for application in agriculture as biostimulants. RESULTS: Quinoa extract was obtained and characterized by liquid chromatography with tandem mass spectrometry (LC–MS/MS). Sixteen saponin congeners were successfully identified and quantified. The QE obtained was used as a reducing agent for silver ions to synthesize silver nanoparticles (QEAgNPs) under mild conditions. The morphology, particle size, and stability of Ag nanoparticles were investigated by transmission electron microscopy (TEM), ultraviolet–visible spectroscopy (UV-visible), energy-dispersive X-ray (EDS), zeta potential, and Fourier transform infrared spectroscopy with attenuated total reflection (FTIR-ATR). Ultraviolet–visible spectroscopy measurements confirmed the formation of silver nanoparticles in the presence of QE, with estimated particle sizes in a range between 5 and 50 nm. According to the zeta potential values, highly stable nanoparticles were formed. The QE and QEAgNPs (200–1000 μg/mL) were also tested in radish seed bioassay to evaluate their phytotoxicity. The seed germination assays revealed that QEAgNPs possessed a phytostimulant effect on radish seeds in a dose-dependent manner, and no phytotoxicity was observed for both QE and QEAgNPs. CONCLUSION: Silver nanoparticles obtained by a so-called ‘green’ method could be considered as good candidates for application in the agricultural sector for seed treatment, or as foliar sprays and plant-growth-promoters.",
journal = "Journal of the Science of Food and Agriculture",
title = "Phytostimulant properties of highly stable silver nanoparticles obtained with saponin extract from Chenopodium quinoa",
volume = "100",
number = "13",
pages = "4987-4994",
doi = "10.1002/jsfa.10529"
}

Segura, R., Vásquez, G., Colson, E., Gerbaux, P., Frischmon, C., Nešić, A., García, D. E.,& Cabrera‐Barjas, G.. (2020). Phytostimulant properties of highly stable silver nanoparticles obtained with saponin extract from Chenopodium quinoa. in Journal of the Science of Food and Agriculture, 100(13), 4987-4994.
https://doi.org/10.1002/jsfa.10529

Segura R, Vásquez G, Colson E, Gerbaux P, Frischmon C, Nešić A, García DE, Cabrera‐Barjas G. Phytostimulant properties of highly stable silver nanoparticles obtained with saponin extract from Chenopodium quinoa. in Journal of the Science of Food and Agriculture. 2020;100(13):4987-4994.
doi:10.1002/jsfa.10529 .

Segura, Rodrigo, Vásquez, Gustavo, Colson, Emmanuel, Gerbaux, Pascal, Frischmon, Caroline, Nešić, Aleksandra, García, Danni E., Cabrera‐Barjas, Gustavo, "Phytostimulant properties of highly stable silver nanoparticles obtained with saponin extract from Chenopodium quinoa" in Journal of the Science of Food and Agriculture, 100, no. 13 (2020):4987-4994,
https://doi.org/10.1002/jsfa.10529 . .