TY  - JOUR
AU  - Bernabé, Patricia
AU  - Becherán, Liliam
AU  - Cabrera-Barjas, Gustavo
AU  - Nešić, Aleksandra
AU  - Alburquenque, Claudio
AU  - Tapia, Cecilia V.
AU  - Taboada, Edelio
AU  - Alderete, Joel
AU  - De Los Ríos, Patricio
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10702
AB  - In the present work, the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton was studied for the first time. α-Chitin was isolated from three main body parts (pincers, legs, carapace), and its content ranged from 9.0–10.4% (w/w). Moreover, chitosan was extracted by alkaline treatment at different temperature and time regimes. Aegla cholchol exoskeleton, chitin and chitosans were characterized by FTIR, TGA, and SEM. Chemical structure of chitin and chitosan was confirmed by NMR spectroscopy. Chitosan molecular weight determinations were carried out by GPC. The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65–201 kDa. The antifungal activity of the chitosan samples and the chitooligomer were tested toward twenty isolated clinical strains of Candida yeast. Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis. Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.
T2  - International Journal of Biological Macromolecules
T1  - Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp
VL  - 149
SP  - 962
EP  - 975
DO  - 10.1016/j.ijbiomac.2020.01.126
ER  - 

@article{
author = "Bernabé, Patricia and Becherán, Liliam and Cabrera-Barjas, Gustavo and Nešić, Aleksandra and Alburquenque, Claudio and Tapia, Cecilia V. and Taboada, Edelio and Alderete, Joel and De Los Ríos, Patricio",
year = "2020",
abstract = "In the present work, the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton was studied for the first time. α-Chitin was isolated from three main body parts (pincers, legs, carapace), and its content ranged from 9.0–10.4% (w/w). Moreover, chitosan was extracted by alkaline treatment at different temperature and time regimes. Aegla cholchol exoskeleton, chitin and chitosans were characterized by FTIR, TGA, and SEM. Chemical structure of chitin and chitosan was confirmed by NMR spectroscopy. Chitosan molecular weight determinations were carried out by GPC. The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65–201 kDa. The antifungal activity of the chitosan samples and the chitooligomer were tested toward twenty isolated clinical strains of Candida yeast. Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis. Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.",
journal = "International Journal of Biological Macromolecules",
title = "Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp",
volume = "149",
pages = "962-975",
doi = "10.1016/j.ijbiomac.2020.01.126"
}

Bernabé, P., Becherán, L., Cabrera-Barjas, G., Nešić, A., Alburquenque, C., Tapia, C. V., Taboada, E., Alderete, J.,& De Los Ríos, P.. (2020). Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp. in International Journal of Biological Macromolecules, 149, 962-975.
https://doi.org/10.1016/j.ijbiomac.2020.01.126

Bernabé P, Becherán L, Cabrera-Barjas G, Nešić A, Alburquenque C, Tapia CV, Taboada E, Alderete J, De Los Ríos P. Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp. in International Journal of Biological Macromolecules. 2020;149:962-975.
doi:10.1016/j.ijbiomac.2020.01.126 .

Bernabé, Patricia, Becherán, Liliam, Cabrera-Barjas, Gustavo, Nešić, Aleksandra, Alburquenque, Claudio, Tapia, Cecilia V., Taboada, Edelio, Alderete, Joel, De Los Ríos, Patricio, "Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp" in International Journal of Biological Macromolecules, 149 (2020):962-975,
https://doi.org/10.1016/j.ijbiomac.2020.01.126 . .