TY  - JOUR
AU  - Mačvanin, Mirjana
AU  - Gluvić, Zoran
AU  - Zafirović, Sonja
AU  - Gao, Xin
AU  - Essack, Magbubah
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10613
AB  - An imbalance between pro-oxidative and antioxidative cellular mechanisms is oxidative stress (OxS) which may be systemic or organ-specific. Although OxS is a consequence of normal body and organ physiology, severely impaired oxidative homeostasis results in DNA hydroxylation, protein denaturation, lipid peroxidation, and apoptosis, ultimately compromising cells’ function and viability. The thyroid gland is an organ that exhibits both oxidative and antioxidative processes. In terms of OxS severity, the thyroid gland’s response could be physiological (i.e. hormone production and secretion) or pathological (i.e. development of diseases, such as goitre, thyroid cancer, or thyroiditis). Protective nutritional antioxidants may benefit defensive antioxidative systems in resolving pro-oxidative dominance and redox imbalance, preventing or delaying chronic thyroid diseases. This review provides information on nutritional antioxidants and their protective roles against impaired redox homeostasis in various thyroid pathologies. We also review novel findings related to the connection between the thyroid gland and gut microbiome and analyze the effects of probiotics with antioxidant properties on thyroid diseases. Copyright © 2023 Macvanin, Gluvic, Zafirovic, Gao, Essack and Isenovic.
T2  - Frontiers in Endocrinology
T1  - The protective role of nutritional antioxidants against oxidative stress in thyroid disorders
VL  - 13
DO  - 10.3389/fendo.2022.1092837
ER  - 

@article{
author = "Mačvanin, Mirjana and Gluvić, Zoran and Zafirović, Sonja and Gao, Xin and Essack, Magbubah and Isenović, Esma R.",
year = "2023",
abstract = "An imbalance between pro-oxidative and antioxidative cellular mechanisms is oxidative stress (OxS) which may be systemic or organ-specific. Although OxS is a consequence of normal body and organ physiology, severely impaired oxidative homeostasis results in DNA hydroxylation, protein denaturation, lipid peroxidation, and apoptosis, ultimately compromising cells’ function and viability. The thyroid gland is an organ that exhibits both oxidative and antioxidative processes. In terms of OxS severity, the thyroid gland’s response could be physiological (i.e. hormone production and secretion) or pathological (i.e. development of diseases, such as goitre, thyroid cancer, or thyroiditis). Protective nutritional antioxidants may benefit defensive antioxidative systems in resolving pro-oxidative dominance and redox imbalance, preventing or delaying chronic thyroid diseases. This review provides information on nutritional antioxidants and their protective roles against impaired redox homeostasis in various thyroid pathologies. We also review novel findings related to the connection between the thyroid gland and gut microbiome and analyze the effects of probiotics with antioxidant properties on thyroid diseases. Copyright © 2023 Macvanin, Gluvic, Zafirovic, Gao, Essack and Isenovic.",
journal = "Frontiers in Endocrinology",
title = "The protective role of nutritional antioxidants against oxidative stress in thyroid disorders",
volume = "13",
doi = "10.3389/fendo.2022.1092837"
}

Mačvanin, M., Gluvić, Z., Zafirović, S., Gao, X., Essack, M.,& Isenović, E. R.. (2023). The protective role of nutritional antioxidants against oxidative stress in thyroid disorders. in Frontiers in Endocrinology, 13.
https://doi.org/10.3389/fendo.2022.1092837

Mačvanin M, Gluvić Z, Zafirović S, Gao X, Essack M, Isenović ER. The protective role of nutritional antioxidants against oxidative stress in thyroid disorders. in Frontiers in Endocrinology. 2023;13.
doi:10.3389/fendo.2022.1092837 .

Mačvanin, Mirjana, Gluvić, Zoran, Zafirović, Sonja, Gao, Xin, Essack, Magbubah, Isenović, Esma R., "The protective role of nutritional antioxidants against oxidative stress in thyroid disorders" in Frontiers in Endocrinology, 13 (2023),
https://doi.org/10.3389/fendo.2022.1092837 . .

TY  - JOUR
AU  - Tomasović, M.
AU  - Sinik, M.
AU  - Gluvić, Zoran
AU  - Zafirović, Sonja
AU  - Isenović, Esma
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12878
AB  - Context. Propylthiouracil (PTU) could cause lupus or vasculitis-like hypersensitivities thus interfering with some other concomitant diseases. Objective. Clinicians must be aware of the side effects of medications, particularly after their introduction and long-term use. Some clinical manifestations may be similar to well-known drug side effects or hypersensitivity. Every unusual clinical scenario related to drug use must be evaluated individually and thoroughly. Subjects and Methods. Hands and feet skin changes were observed several days after PTU administration in a male patient with severe diffuse toxic goitre. A complete blood count, biochemistry analyses, thyroid function tests and antibodies, and immunology analyses were performed. Results. As the skin changes were distributed regionally, liver function tests were normal, and there were no signs of clinical deterioration, it was decided to continue PTU treatment and monitor the patient. The initial maculopapular rash quickly turned vesicular, then scaly. After two weeks, the skin changes were wholly restored, with no scarring. Hand, Foot, and Mouth disease (HFMD) was diagnosed after a thorough epidemiological survey and clinical workout. Conclusions. Our case study demonstrates that skin changes associated with HFMD may resemble those associated with PTU-induced vasculitis.
T2  - Acta Endocrinologica (Bucharest)
T1  - Case Report of Hand and Foot Skin Changes Resembling PTU-Induced Vasculitis in a Young Male with Diffuse Toxic Goitre
VL  - 19
IS  - 3
SP  - 380
EP  - 385
DO  - 10.4183/aeb.2023.380
ER  - 

@article{
author = "Tomasović, M. and Sinik, M. and Gluvić, Zoran and Zafirović, Sonja and Isenović, Esma",
year = "2023",
abstract = "Context. Propylthiouracil (PTU) could cause lupus or vasculitis-like hypersensitivities thus interfering with some other concomitant diseases. Objective. Clinicians must be aware of the side effects of medications, particularly after their introduction and long-term use. Some clinical manifestations may be similar to well-known drug side effects or hypersensitivity. Every unusual clinical scenario related to drug use must be evaluated individually and thoroughly. Subjects and Methods. Hands and feet skin changes were observed several days after PTU administration in a male patient with severe diffuse toxic goitre. A complete blood count, biochemistry analyses, thyroid function tests and antibodies, and immunology analyses were performed. Results. As the skin changes were distributed regionally, liver function tests were normal, and there were no signs of clinical deterioration, it was decided to continue PTU treatment and monitor the patient. The initial maculopapular rash quickly turned vesicular, then scaly. After two weeks, the skin changes were wholly restored, with no scarring. Hand, Foot, and Mouth disease (HFMD) was diagnosed after a thorough epidemiological survey and clinical workout. Conclusions. Our case study demonstrates that skin changes associated with HFMD may resemble those associated with PTU-induced vasculitis.",
journal = "Acta Endocrinologica (Bucharest)",
title = "Case Report of Hand and Foot Skin Changes Resembling PTU-Induced Vasculitis in a Young Male with Diffuse Toxic Goitre",
volume = "19",
number = "3",
pages = "380-385",
doi = "10.4183/aeb.2023.380"
}

Tomasović, M., Sinik, M., Gluvić, Z., Zafirović, S.,& Isenović, E.. (2023). Case Report of Hand and Foot Skin Changes Resembling PTU-Induced Vasculitis in a Young Male with Diffuse Toxic Goitre. in Acta Endocrinologica (Bucharest), 19(3), 380-385.
https://doi.org/10.4183/aeb.2023.380

Tomasović M, Sinik M, Gluvić Z, Zafirović S, Isenović E. Case Report of Hand and Foot Skin Changes Resembling PTU-Induced Vasculitis in a Young Male with Diffuse Toxic Goitre. in Acta Endocrinologica (Bucharest). 2023;19(3):380-385.
doi:10.4183/aeb.2023.380 .

Tomasović, M., Sinik, M., Gluvić, Zoran, Zafirović, Sonja, Isenović, Esma, "Case Report of Hand and Foot Skin Changes Resembling PTU-Induced Vasculitis in a Young Male with Diffuse Toxic Goitre" in Acta Endocrinologica (Bucharest), 19, no. 3 (2023):380-385,
https://doi.org/10.4183/aeb.2023.380 . .

TY  - JOUR
AU  - Mačvanin, Mirjana
AU  - Zafirović, Sonja
AU  - Obradović, Milan M.
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10687
T2  - Frontiers in Endocrinology
T1  - Editorial: Non-coding RNA in diabetes and cardiovascular diseases
VL  - 14
DO  - 10.3389/fendo.2023.1149857
ER  - 

@article{
author = "Mačvanin, Mirjana and Zafirović, Sonja and Obradović, Milan M. and Isenović, Esma R.",
year = "2023",
journal = "Frontiers in Endocrinology",
title = "Editorial: Non-coding RNA in diabetes and cardiovascular diseases",
volume = "14",
doi = "10.3389/fendo.2023.1149857"
}

Mačvanin, M., Zafirović, S., Obradović, M. M.,& Isenović, E. R.. (2023). Editorial: Non-coding RNA in diabetes and cardiovascular diseases. in Frontiers in Endocrinology, 14.
https://doi.org/10.3389/fendo.2023.1149857

Mačvanin M, Zafirović S, Obradović MM, Isenović ER. Editorial: Non-coding RNA in diabetes and cardiovascular diseases. in Frontiers in Endocrinology. 2023;14.
doi:10.3389/fendo.2023.1149857 .

Mačvanin, Mirjana, Zafirović, Sonja, Obradović, Milan M., Isenović, Esma R., "Editorial: Non-coding RNA in diabetes and cardiovascular diseases" in Frontiers in Endocrinology, 14 (2023),
https://doi.org/10.3389/fendo.2023.1149857 . .

TY  - JOUR
AU  - Panić, Anastasija
AU  - Sudar-Milovanović, Emina
AU  - Stanimirović, Julijana
AU  - Obradović, Milan M.
AU  - Zafirović, Sonja
AU  - Soskić, Sanja S.
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10744
AB  - The liver is an organ in which many oxidative processes occur and represents an important target of oxidative stress (OxS). Under physiological conditions of normal mitochondrial homeostasis, hepatocytes effectively remove reactive oxygen species (ROS) by enabling metabolic adaptations and through the antioxidant defence system mechanisms. However, obesity-induced lipid accumulation in the hepatocytes causes significantly elevated production of ROS, reduces oxidative capacity, and increases oxidative stress (OxS). In men, compared with premenopausal women, the development of insulin resistance (IR) and non-alcoholic fatty liver disease (NAFLD) in obesity are more prevalent, where oestradiol (E2), the most potent female sex steroid, is proposed as the main culprit. Exogenous oestradiol (E2) administration exerts beneficial effects on antioxidant properties, restores total plasma antioxidant capacity and decreases biomarkers of OxS in ovariectomized animal models. Thus, we hypothesized that E2 treatment in states of obesity could have beneficial effects against OxS in the obese male's liver. We assumed that E2 could directly affect the level of OxS by increasing the level/activity of the AOS enzymes, particularly SOD1, SOD2, GPx, and CAT, in obese males' livers. In addition, we assumed that the level of malondialdehyde (MDA) and protein carbonyl content (PCC) in obese males' livers would be reduced after E2 treatment as a result of E2 inhibitory effect on lipid peroxidation and protein oxidation, respectively. To test our hypothesis, we used the liver of a high-fat (HF) diet-fed male Wistar rats as an animal model of obesity, treated with E2 intraperitoneally (40 μg/kg). Preliminary results from this study support our hypothesis that E2 increases liver protein expression of AOS enzymes: SOD1, GPx, and CAT, in control and HF male rats compared with their respective controls. The protein level of SOD2 and CAT activity was increased in HF treated with E2 compared with non-treated HF rats. Moreover, as we expected, E2 administration significantly decreased the MDA level in both E2-treated groups compared to their controls, while the PCC level was significantly decreased in HF treated group compared with untreated HF rats. In conclusion, the preliminary results we obtained in this study indicate that E2 administration can effectively inhibit the OxS-related processes in the liver in HF diet-induced obesity by increasing AOS enzymes levels and CAT activity, and also by decreasing levels of MDA and PCC. A consequence of our hypothesis is that treatment with E2 may be an innovative way to improve obesity-related liver disease prevention and healing. © 2023 Elsevier Ltd
T2  - Medical Hypotheses
T1  - Does oestradiol treatment alleviate obesity-induced oxidative stress in the male liver?
VL  - 174
SP  - 111049
DO  - 10.1016/j.mehy.2023.111049
ER  - 

@article{
author = "Panić, Anastasija and Sudar-Milovanović, Emina and Stanimirović, Julijana and Obradović, Milan M. and Zafirović, Sonja and Soskić, Sanja S. and Isenović, Esma R.",
year = "2023",
abstract = "The liver is an organ in which many oxidative processes occur and represents an important target of oxidative stress (OxS). Under physiological conditions of normal mitochondrial homeostasis, hepatocytes effectively remove reactive oxygen species (ROS) by enabling metabolic adaptations and through the antioxidant defence system mechanisms. However, obesity-induced lipid accumulation in the hepatocytes causes significantly elevated production of ROS, reduces oxidative capacity, and increases oxidative stress (OxS). In men, compared with premenopausal women, the development of insulin resistance (IR) and non-alcoholic fatty liver disease (NAFLD) in obesity are more prevalent, where oestradiol (E2), the most potent female sex steroid, is proposed as the main culprit. Exogenous oestradiol (E2) administration exerts beneficial effects on antioxidant properties, restores total plasma antioxidant capacity and decreases biomarkers of OxS in ovariectomized animal models. Thus, we hypothesized that E2 treatment in states of obesity could have beneficial effects against OxS in the obese male's liver. We assumed that E2 could directly affect the level of OxS by increasing the level/activity of the AOS enzymes, particularly SOD1, SOD2, GPx, and CAT, in obese males' livers. In addition, we assumed that the level of malondialdehyde (MDA) and protein carbonyl content (PCC) in obese males' livers would be reduced after E2 treatment as a result of E2 inhibitory effect on lipid peroxidation and protein oxidation, respectively. To test our hypothesis, we used the liver of a high-fat (HF) diet-fed male Wistar rats as an animal model of obesity, treated with E2 intraperitoneally (40 μg/kg). Preliminary results from this study support our hypothesis that E2 increases liver protein expression of AOS enzymes: SOD1, GPx, and CAT, in control and HF male rats compared with their respective controls. The protein level of SOD2 and CAT activity was increased in HF treated with E2 compared with non-treated HF rats. Moreover, as we expected, E2 administration significantly decreased the MDA level in both E2-treated groups compared to their controls, while the PCC level was significantly decreased in HF treated group compared with untreated HF rats. In conclusion, the preliminary results we obtained in this study indicate that E2 administration can effectively inhibit the OxS-related processes in the liver in HF diet-induced obesity by increasing AOS enzymes levels and CAT activity, and also by decreasing levels of MDA and PCC. A consequence of our hypothesis is that treatment with E2 may be an innovative way to improve obesity-related liver disease prevention and healing. © 2023 Elsevier Ltd",
journal = "Medical Hypotheses",
title = "Does oestradiol treatment alleviate obesity-induced oxidative stress in the male liver?",
volume = "174",
pages = "111049",
doi = "10.1016/j.mehy.2023.111049"
}

Panić, A., Sudar-Milovanović, E., Stanimirović, J., Obradović, M. M., Zafirović, S., Soskić, S. S.,& Isenović, E. R.. (2023). Does oestradiol treatment alleviate obesity-induced oxidative stress in the male liver?. in Medical Hypotheses, 174, 111049.
https://doi.org/10.1016/j.mehy.2023.111049

Panić A, Sudar-Milovanović E, Stanimirović J, Obradović MM, Zafirović S, Soskić SS, Isenović ER. Does oestradiol treatment alleviate obesity-induced oxidative stress in the male liver?. in Medical Hypotheses. 2023;174:111049.
doi:10.1016/j.mehy.2023.111049 .

Panić, Anastasija, Sudar-Milovanović, Emina, Stanimirović, Julijana, Obradović, Milan M., Zafirović, Sonja, Soskić, Sanja S., Isenović, Esma R., "Does oestradiol treatment alleviate obesity-induced oxidative stress in the male liver?" in Medical Hypotheses, 174 (2023):111049,
https://doi.org/10.1016/j.mehy.2023.111049 . .

TY  - JOUR
AU  - Mačvanin, Mirjana
AU  - Obradović, Milan M.
AU  - Zafirović, Sonja
AU  - Stanimirović, Julijana
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10757
AB  - Metabolic diseases such as obesity, diabetes, dyslipidemia, and insulin resistance are characterized by glucose and lipid metabolism alterations and represent a global health problem. Many studies have established the crucial role of micro-ribonucleic acids (miRNAs) in controlling metabolic processes in various tissues. miRNAs are single-stranded, highly conserved non-coding RNAs containing 20-24 oligonucleotides that are expressed in a tissue-specific manner. miRNAs mainly interact through base pairing with 3' untranslated regions of target gene mRNAs to promote inhibition of their translation. miRNAs regulate the expression of as many as 30% of the human genes and have a role in crucial physiological processes such as human growth and development, cell proliferation, apoptosis, and metabolism. The number of miRNA molecules with a confirmed role in the pathogenesis of metabolic diseases is quickly expanding due to the availability of high-throughput methodologies for their identification. In this review, we present recent findings regarding the role of miRNAs as endocrine signaling molecules involved in the regulation of insulin production and fat metabolism. We discuss the potential of extracellular miRNAs present in biological fluids miRNAs as biomarkers for the prediction of diabetes and MetS. We also give an updated overview of therapeutic interventions based on antisense oligonucleotides and the CRISPR/Cas9 editing platform for manipulating levels of miRNAs involved in metabolic disorders. © 2023 Bentham Science Publishers.
T2  - Current Medicinal Chemistry
T1  - The Role of miRNAs in Metabolic Diseases
VL  - 30
IS  - 17
SP  - 1922
EP  - 1944
DO  - 10.2174/0929867329666220801161536
ER  - 

@article{
author = "Mačvanin, Mirjana and Obradović, Milan M. and Zafirović, Sonja and Stanimirović, Julijana and Isenović, Esma R.",
year = "2023",
abstract = "Metabolic diseases such as obesity, diabetes, dyslipidemia, and insulin resistance are characterized by glucose and lipid metabolism alterations and represent a global health problem. Many studies have established the crucial role of micro-ribonucleic acids (miRNAs) in controlling metabolic processes in various tissues. miRNAs are single-stranded, highly conserved non-coding RNAs containing 20-24 oligonucleotides that are expressed in a tissue-specific manner. miRNAs mainly interact through base pairing with 3' untranslated regions of target gene mRNAs to promote inhibition of their translation. miRNAs regulate the expression of as many as 30% of the human genes and have a role in crucial physiological processes such as human growth and development, cell proliferation, apoptosis, and metabolism. The number of miRNA molecules with a confirmed role in the pathogenesis of metabolic diseases is quickly expanding due to the availability of high-throughput methodologies for their identification. In this review, we present recent findings regarding the role of miRNAs as endocrine signaling molecules involved in the regulation of insulin production and fat metabolism. We discuss the potential of extracellular miRNAs present in biological fluids miRNAs as biomarkers for the prediction of diabetes and MetS. We also give an updated overview of therapeutic interventions based on antisense oligonucleotides and the CRISPR/Cas9 editing platform for manipulating levels of miRNAs involved in metabolic disorders. © 2023 Bentham Science Publishers.",
journal = "Current Medicinal Chemistry",
title = "The Role of miRNAs in Metabolic Diseases",
volume = "30",
number = "17",
pages = "1922-1944",
doi = "10.2174/0929867329666220801161536"
}

Mačvanin, M., Obradović, M. M., Zafirović, S., Stanimirović, J.,& Isenović, E. R.. (2023). The Role of miRNAs in Metabolic Diseases. in Current Medicinal Chemistry, 30(17), 1922-1944.
https://doi.org/10.2174/0929867329666220801161536

Mačvanin M, Obradović MM, Zafirović S, Stanimirović J, Isenović ER. The Role of miRNAs in Metabolic Diseases. in Current Medicinal Chemistry. 2023;30(17):1922-1944.
doi:10.2174/0929867329666220801161536 .

Mačvanin, Mirjana, Obradović, Milan M., Zafirović, Sonja, Stanimirović, Julijana, Isenović, Esma R., "The Role of miRNAs in Metabolic Diseases" in Current Medicinal Chemistry, 30, no. 17 (2023):1922-1944,
https://doi.org/10.2174/0929867329666220801161536 . .

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Samardžić, Vladimir
AU  - Mačvanin, Mirjana
AU  - Zafirović, Sonja
AU  - Gluvić, Zoran
AU  - Grubin, Jasmina
AU  - Gao, Xin
AU  - Essack, Magbubah
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11567
AB  - Thyroid nodules (TN) are localized morphological changes in the thyroid gland and can be benign or malignant.Objective: The present study investigates the relationships between biochemical markers in serum (s) and their homologs in washout (w) after fine-needle aspiration biopsy (FNAB) of the TN of interest and their correlation with cytology specimen findings.We investigated the relationships between serum biochemical markers nitric oxide (NO), thyroglobulin (TG), and calcitonin (CT), their homologs in washout after FNAB of the TN of interest, and cytology findings of biopsy samples classified according to the Bethesda system for thyroid cytopathology in this study, which included 86 subjects.Results: Washout TG (TGw) level positively correlates with the cytology finding of the biopsy. A higher level of TGw correlates with higher categories of the Bethesda classification and indicates a higher malignant potential. The levels of serum NO (NOs), serum TG (TGs), serum CT (CTs), and washout CT (CTw) do not correlate with the cytology finding of the biopsy, and the higher levels of washout NO (NOw) correspond to the more suspicious ultrasound findings.The findings of our study suggest that TGw and NOw could be used as potential predictors of malignancy in TN.
T2  - Frontiers in Endocrinology
T1  - Nitric oxide, thyroglobulin, and calcitonin: Unravelling the nature of thyroid nodules
VL  - 14
SP  - 1241223
DO  - 10.3389/fendo.2023.1241223
ER  - 

@article{
author = "Obradović, Milan M. and Samardžić, Vladimir and Mačvanin, Mirjana and Zafirović, Sonja and Gluvić, Zoran and Grubin, Jasmina and Gao, Xin and Essack, Magbubah and Isenović, Esma R.",
year = "2023",
abstract = "Thyroid nodules (TN) are localized morphological changes in the thyroid gland and can be benign or malignant.Objective: The present study investigates the relationships between biochemical markers in serum (s) and their homologs in washout (w) after fine-needle aspiration biopsy (FNAB) of the TN of interest and their correlation with cytology specimen findings.We investigated the relationships between serum biochemical markers nitric oxide (NO), thyroglobulin (TG), and calcitonin (CT), their homologs in washout after FNAB of the TN of interest, and cytology findings of biopsy samples classified according to the Bethesda system for thyroid cytopathology in this study, which included 86 subjects.Results: Washout TG (TGw) level positively correlates with the cytology finding of the biopsy. A higher level of TGw correlates with higher categories of the Bethesda classification and indicates a higher malignant potential. The levels of serum NO (NOs), serum TG (TGs), serum CT (CTs), and washout CT (CTw) do not correlate with the cytology finding of the biopsy, and the higher levels of washout NO (NOw) correspond to the more suspicious ultrasound findings.The findings of our study suggest that TGw and NOw could be used as potential predictors of malignancy in TN.",
journal = "Frontiers in Endocrinology",
title = "Nitric oxide, thyroglobulin, and calcitonin: Unravelling the nature of thyroid nodules",
volume = "14",
pages = "1241223",
doi = "10.3389/fendo.2023.1241223"
}

Obradović, M. M., Samardžić, V., Mačvanin, M., Zafirović, S., Gluvić, Z., Grubin, J., Gao, X., Essack, M.,& Isenović, E. R.. (2023). Nitric oxide, thyroglobulin, and calcitonin: Unravelling the nature of thyroid nodules. in Frontiers in Endocrinology, 14, 1241223.
https://doi.org/10.3389/fendo.2023.1241223

Obradović MM, Samardžić V, Mačvanin M, Zafirović S, Gluvić Z, Grubin J, Gao X, Essack M, Isenović ER. Nitric oxide, thyroglobulin, and calcitonin: Unravelling the nature of thyroid nodules. in Frontiers in Endocrinology. 2023;14:1241223.
doi:10.3389/fendo.2023.1241223 .

Obradović, Milan M., Samardžić, Vladimir, Mačvanin, Mirjana, Zafirović, Sonja, Gluvić, Zoran, Grubin, Jasmina, Gao, Xin, Essack, Magbubah, Isenović, Esma R., "Nitric oxide, thyroglobulin, and calcitonin: Unravelling the nature of thyroid nodules" in Frontiers in Endocrinology, 14 (2023):1241223,
https://doi.org/10.3389/fendo.2023.1241223 . .

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Zafirović, Sonja
AU  - Gluvić, Zoran
AU  - Radovanović, Jelena
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11639
AB  - The current literature findings on autophagy’s beneficial and detrimental roles in diabetes mellitus (DM) and diabetes-related comorbidities were reviewed. The effects of oral hypoglycaemic medicines and autophagy in DM. Autophagy plays an important function in cellular homeostasis by promoting cell survival or initiating cell death in physiological settings was also assessed. Although autophagy protects insulin-target tissues, organelle failure caused by autophagy malfunction influences DM and other metabolic diseases. Endoplasmic reticulum and oxidative stress enhance autophagy levels, making it easier to regulate stress-induced intracellular changes. Evidence suggests that autophagy-caused cell death can occur when autophagy is overstimulated and constitutively activated, which might prevent or develop DM. Even though the precise role of autophagy in DM complications is uncertain, deregulation of the autophagic machinery is strongly linked to beta cell destruction and the aetiology of DM. Thus, improving autophagy dysfunction is a possible therapeutic objective in treating DM and other metabolic disorders.
T2  - Exploration of Medicine
T1  - Autophagy and diabetes
SP  - 576
EP  - 588
DO  - 10.37349/emed.2023.00162
ER  - 

@article{
author = "Obradović, Milan M. and Zafirović, Sonja and Gluvić, Zoran and Radovanović, Jelena and Isenović, Esma R.",
year = "2023",
abstract = "The current literature findings on autophagy’s beneficial and detrimental roles in diabetes mellitus (DM) and diabetes-related comorbidities were reviewed. The effects of oral hypoglycaemic medicines and autophagy in DM. Autophagy plays an important function in cellular homeostasis by promoting cell survival or initiating cell death in physiological settings was also assessed. Although autophagy protects insulin-target tissues, organelle failure caused by autophagy malfunction influences DM and other metabolic diseases. Endoplasmic reticulum and oxidative stress enhance autophagy levels, making it easier to regulate stress-induced intracellular changes. Evidence suggests that autophagy-caused cell death can occur when autophagy is overstimulated and constitutively activated, which might prevent or develop DM. Even though the precise role of autophagy in DM complications is uncertain, deregulation of the autophagic machinery is strongly linked to beta cell destruction and the aetiology of DM. Thus, improving autophagy dysfunction is a possible therapeutic objective in treating DM and other metabolic disorders.",
journal = "Exploration of Medicine",
title = "Autophagy and diabetes",
pages = "576-588",
doi = "10.37349/emed.2023.00162"
}

Obradović, M. M., Zafirović, S., Gluvić, Z., Radovanović, J.,& Isenović, E. R.. (2023). Autophagy and diabetes. in Exploration of Medicine, 576-588.
https://doi.org/10.37349/emed.2023.00162

Obradović MM, Zafirović S, Gluvić Z, Radovanović J, Isenović ER. Autophagy and diabetes. in Exploration of Medicine. 2023;:576-588.
doi:10.37349/emed.2023.00162 .

Obradović, Milan M., Zafirović, Sonja, Gluvić, Zoran, Radovanović, Jelena, Isenović, Esma R., "Autophagy and diabetes" in Exploration of Medicine (2023):576-588,
https://doi.org/10.37349/emed.2023.00162 . .

TY  - JOUR
AU  - Tomić, Aleksandra
AU  - Zafirović, Sonja
AU  - Gluvić, Zoran
AU  - Samardžić, Vladimir
AU  - Mačvanin, Mirjana
AU  - Radunović, Maja
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11875
AB  - Background:Subacute thyroiditis (SAT) is an organ-specific disease that various drugs, including COVID-19vaccines, can trigger. COVID-19 infection has been associated with thyroid gland damage and disease SARS-CoV-2direct action, euthyroid sick syndrome, and immune-mediated mechanisms are all potential mechanisms of thyroiddamage. It denotes thyroid gland inflammation, most commonly of viral origin, and belongs to the transitory, self-limiting thyroid gland diseases group, causing complications in approximately 15% of patients in the formof permanent hypothyroidism. Some authors say SAT is the most common thyroid disease associated withCOVID-19.Purpose:The occurrence of SAT many weeks after administering the second COVID-19 vaccine is rare and has limiteddocumentation in academic literature. This study aims to present the occurrence of SAT after administering the COVID-19vaccine. We present the case of a 37-year-old man who developed SAT 23 days after receiving the second dose of PfizerBioNTech’s COVID-19 mRNA vaccine.Research design and study sample:Due to neck pain and an elevated body temperature (up to 38.2°C), a 37-year-old male subject presented for examination 23 days after receiving the second Pfizer BioNTech mRNA vaccineagainst SARS-CoV-2 viral infection. The patient deniedever having an autoimmune disease or any other disease.Painful neck palpation and afirm, slightly enlarged thyroid gland with no surrounding lymphadenopathy wereidentified during the exam. The heart rate was 104 beatsper minute. All of the remaining physicalfindings werenormal.Data collection and/or Analysis:Data collected during the disease are integral to the medical record.Results:Hematology and biochemistry analyses at the initial and follow-up visits revealed minor leukocytosis, normocyticanaemia, and thrombocytosis, followed by a mild increase in lactate dehydrogenase and decreased iron levels. The patient’sthyroid function and morphology had recovered entirely from post-vaccine SAT.Conclusions: Results from this study emphasise the need for healthcare professionals to promptly report any case of SATrelated to COVID-19 vaccination. Further investigation is warranted to understand the immunopathogenesis of COVID-19-associated thyroiditis and the impact of COVID-19 immunization on this condition.
T2  - Antiviral Therapy
T1  - Subacute thyroiditis following COVID-19 vaccination: Case presentation
VL  - 28
IS  - 5
DO  - 10.1177/13596535231208831
ER  - 

@article{
author = "Tomić, Aleksandra and Zafirović, Sonja and Gluvić, Zoran and Samardžić, Vladimir and Mačvanin, Mirjana and Radunović, Maja and Isenović, Esma R.",
year = "2023",
abstract = "Background:Subacute thyroiditis (SAT) is an organ-specific disease that various drugs, including COVID-19vaccines, can trigger. COVID-19 infection has been associated with thyroid gland damage and disease SARS-CoV-2direct action, euthyroid sick syndrome, and immune-mediated mechanisms are all potential mechanisms of thyroiddamage. It denotes thyroid gland inflammation, most commonly of viral origin, and belongs to the transitory, self-limiting thyroid gland diseases group, causing complications in approximately 15% of patients in the formof permanent hypothyroidism. Some authors say SAT is the most common thyroid disease associated withCOVID-19.Purpose:The occurrence of SAT many weeks after administering the second COVID-19 vaccine is rare and has limiteddocumentation in academic literature. This study aims to present the occurrence of SAT after administering the COVID-19vaccine. We present the case of a 37-year-old man who developed SAT 23 days after receiving the second dose of PfizerBioNTech’s COVID-19 mRNA vaccine.Research design and study sample:Due to neck pain and an elevated body temperature (up to 38.2°C), a 37-year-old male subject presented for examination 23 days after receiving the second Pfizer BioNTech mRNA vaccineagainst SARS-CoV-2 viral infection. The patient deniedever having an autoimmune disease or any other disease.Painful neck palpation and afirm, slightly enlarged thyroid gland with no surrounding lymphadenopathy wereidentified during the exam. The heart rate was 104 beatsper minute. All of the remaining physicalfindings werenormal.Data collection and/or Analysis:Data collected during the disease are integral to the medical record.Results:Hematology and biochemistry analyses at the initial and follow-up visits revealed minor leukocytosis, normocyticanaemia, and thrombocytosis, followed by a mild increase in lactate dehydrogenase and decreased iron levels. The patient’sthyroid function and morphology had recovered entirely from post-vaccine SAT.Conclusions: Results from this study emphasise the need for healthcare professionals to promptly report any case of SATrelated to COVID-19 vaccination. Further investigation is warranted to understand the immunopathogenesis of COVID-19-associated thyroiditis and the impact of COVID-19 immunization on this condition.",
journal = "Antiviral Therapy",
title = "Subacute thyroiditis following COVID-19 vaccination: Case presentation",
volume = "28",
number = "5",
doi = "10.1177/13596535231208831"
}

Tomić, A., Zafirović, S., Gluvić, Z., Samardžić, V., Mačvanin, M., Radunović, M.,& Isenović, E. R.. (2023). Subacute thyroiditis following COVID-19 vaccination: Case presentation. in Antiviral Therapy, 28(5).
https://doi.org/10.1177/13596535231208831

Tomić A, Zafirović S, Gluvić Z, Samardžić V, Mačvanin M, Radunović M, Isenović ER. Subacute thyroiditis following COVID-19 vaccination: Case presentation. in Antiviral Therapy. 2023;28(5).
doi:10.1177/13596535231208831 .

Tomić, Aleksandra, Zafirović, Sonja, Gluvić, Zoran, Samardžić, Vladimir, Mačvanin, Mirjana, Radunović, Maja, Isenović, Esma R., "Subacute thyroiditis following COVID-19 vaccination: Case presentation" in Antiviral Therapy, 28, no. 5 (2023),
https://doi.org/10.1177/13596535231208831 . .

TY  - JOUR
AU  - Gluvić, Zoran
AU  - Zafirović, Sonja
AU  - Obradović, Milan M.
AU  - Sudar-Milovanović, Emina
AU  - Rizzo, Manfredi
AU  - Isenović, Esma R.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10409
AB  - Thyroid hormones (TH) have a significant impact on cellular oxidative metabolism. Besides that,they maintain vascular homeostasis by positive effects on endothelial and vascular smooth muscle cells. Subclinical(SCH) and clinical (CH) hypothyroidism influences target organs by changing their morphology andfunction and impaired blood and oxygen supply induced by accelerated atherosclerosis. The increased risk ofacceleration and extension of atherosclerosis in patients with SCH and CH could be explained by dyslipidemia,diastolic hypertension, increased arterial stiffness, endothelial dysfunction, and altered blood coagulation. Instabilityof atherosclerotic plaque in hypothyroidism could cause excessive activity of the elements of innateimmunity, which are characterized by the significant presence of macrophages in atherosclerotic plaques, increasednuclear factor kappa B (NFkB) expression, and elevated levels of tumor necrosis factor α (TNF-α) andmatrix metalloproteinase (MMP) 9, with reduced interstitial collagen; all of them together creates inflammationmilieu, resulting in plaque rupture. Optimal substitution by levothyroxine (LT4) restores biochemical euthyroidism.In postmenopausal women and elderly patients with hypothyroidism and associated vascularcomorbidity, excessive LT4 substitution could lead to atrial rhythm disorders and osteoporosis. Therefore, it isof interest to maintain thyroid-stimulating hormone (TSH) levels in the reference range, thus eliminating thedeleterious effects of lower or higher TSH levels on the cardiovascular system. This review summarizes the recentliterature on subclinical and clinical hypothyroidism and atherosclerotic cardiovascular disease and discussesthe effects of LT4 replacement therapy on restoring biochemical euthyroidism and atherosclerosis processes.
T2  - Current Pharmaceutical Design
T1  - Hypothyroidism and Risk of Cardiovascular Disease
VL  - 28
IS  - 25
SP  - 2065
EP  - 2072
DO  - 10.2174/1381612828666220620160516
ER  - 

@article{
author = "Gluvić, Zoran and Zafirović, Sonja and Obradović, Milan M. and Sudar-Milovanović, Emina and Rizzo, Manfredi and Isenović, Esma R.",
year = "2022",
abstract = "Thyroid hormones (TH) have a significant impact on cellular oxidative metabolism. Besides that,they maintain vascular homeostasis by positive effects on endothelial and vascular smooth muscle cells. Subclinical(SCH) and clinical (CH) hypothyroidism influences target organs by changing their morphology andfunction and impaired blood and oxygen supply induced by accelerated atherosclerosis. The increased risk ofacceleration and extension of atherosclerosis in patients with SCH and CH could be explained by dyslipidemia,diastolic hypertension, increased arterial stiffness, endothelial dysfunction, and altered blood coagulation. Instabilityof atherosclerotic plaque in hypothyroidism could cause excessive activity of the elements of innateimmunity, which are characterized by the significant presence of macrophages in atherosclerotic plaques, increasednuclear factor kappa B (NFkB) expression, and elevated levels of tumor necrosis factor α (TNF-α) andmatrix metalloproteinase (MMP) 9, with reduced interstitial collagen; all of them together creates inflammationmilieu, resulting in plaque rupture. Optimal substitution by levothyroxine (LT4) restores biochemical euthyroidism.In postmenopausal women and elderly patients with hypothyroidism and associated vascularcomorbidity, excessive LT4 substitution could lead to atrial rhythm disorders and osteoporosis. Therefore, it isof interest to maintain thyroid-stimulating hormone (TSH) levels in the reference range, thus eliminating thedeleterious effects of lower or higher TSH levels on the cardiovascular system. This review summarizes the recentliterature on subclinical and clinical hypothyroidism and atherosclerotic cardiovascular disease and discussesthe effects of LT4 replacement therapy on restoring biochemical euthyroidism and atherosclerosis processes.",
journal = "Current Pharmaceutical Design",
title = "Hypothyroidism and Risk of Cardiovascular Disease",
volume = "28",
number = "25",
pages = "2065-2072",
doi = "10.2174/1381612828666220620160516"
}

Gluvić, Z., Zafirović, S., Obradović, M. M., Sudar-Milovanović, E., Rizzo, M.,& Isenović, E. R.. (2022). Hypothyroidism and Risk of Cardiovascular Disease. in Current Pharmaceutical Design, 28(25), 2065-2072.
https://doi.org/10.2174/1381612828666220620160516

Gluvić Z, Zafirović S, Obradović MM, Sudar-Milovanović E, Rizzo M, Isenović ER. Hypothyroidism and Risk of Cardiovascular Disease. in Current Pharmaceutical Design. 2022;28(25):2065-2072.
doi:10.2174/1381612828666220620160516 .

Gluvić, Zoran, Zafirović, Sonja, Obradović, Milan M., Sudar-Milovanović, Emina, Rizzo, Manfredi, Isenović, Esma R., "Hypothyroidism and Risk of Cardiovascular Disease" in Current Pharmaceutical Design, 28, no. 25 (2022):2065-2072,
https://doi.org/10.2174/1381612828666220620160516 . .

TY  - CONF
AU  - Tomasović, Martina
AU  - Sinik, Marija
AU  - Joksimović, Bojan
AU  - Lacković, Milena
AU  - Samardžić, Vladimir
AU  - Vujović, Marina
AU  - Gluvić, Zoran
AU  - Obradović, Milan
AU  - Zafirović, Sonja
AU  - Isenović, Esma R.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12029
AB  - Propylthiouracil (PTU) sometimes induces autoimmune syndromes, such as PTU–induced lupus or vasculitis. Here we present hands and feet vasculitis-like skin changes observed several days after PTU introduction in a patient who suffered from serious diffuse toxic goiter. Because of segmental distribution, normal liver function test, and no signs of clinical deterioration, it was decided to continue PTU management and observe the patient. Primarily maculopapular rash became vesicular shortly after and then scaly. After two weeks, skin changes were entirely restored with no scaring. Taking into account thorough epidemiological survey, clinical course, and performed diagnostics, presented skin changes were diagnosed as Hand, Foot, and Mouth disease (HFMD). Clinicians must be aware of the side effects of used drugs, especially after their introduction. Some clinical presentations could only resemble expected or well-known side-effects, intolerance, or hypersensitivity to the used drug. Every clinical presentation associated with any drug introduction must be thoroughly evaluated. The presented case revealed that skin changes of HFMD mimicked PTU-induced vasculitis.
C3  - Endocrine Abstracts
T1  - Hand and foot skin changes resembling PTU-induced vasculitis in a young male with diffuse toxic goiter- a case report
DO  - 10.1530/endoabs.81.EP1030
ER  - 

@conference{
author = "Tomasović, Martina and Sinik, Marija and Joksimović, Bojan and Lacković, Milena and Samardžić, Vladimir and Vujović, Marina and Gluvić, Zoran and Obradović, Milan and Zafirović, Sonja and Isenović, Esma R.",
year = "2022",
abstract = "Propylthiouracil (PTU) sometimes induces autoimmune syndromes, such as PTU–induced lupus or vasculitis. Here we present hands and feet vasculitis-like skin changes observed several days after PTU introduction in a patient who suffered from serious diffuse toxic goiter. Because of segmental distribution, normal liver function test, and no signs of clinical deterioration, it was decided to continue PTU management and observe the patient. Primarily maculopapular rash became vesicular shortly after and then scaly. After two weeks, skin changes were entirely restored with no scaring. Taking into account thorough epidemiological survey, clinical course, and performed diagnostics, presented skin changes were diagnosed as Hand, Foot, and Mouth disease (HFMD). Clinicians must be aware of the side effects of used drugs, especially after their introduction. Some clinical presentations could only resemble expected or well-known side-effects, intolerance, or hypersensitivity to the used drug. Every clinical presentation associated with any drug introduction must be thoroughly evaluated. The presented case revealed that skin changes of HFMD mimicked PTU-induced vasculitis.",
journal = "Endocrine Abstracts",
title = "Hand and foot skin changes resembling PTU-induced vasculitis in a young male with diffuse toxic goiter- a case report",
doi = "10.1530/endoabs.81.EP1030"
}

Tomasović, M., Sinik, M., Joksimović, B., Lacković, M., Samardžić, V., Vujović, M., Gluvić, Z., Obradović, M., Zafirović, S.,& Isenović, E. R.. (2022). Hand and foot skin changes resembling PTU-induced vasculitis in a young male with diffuse toxic goiter- a case report. in Endocrine Abstracts.
https://doi.org/10.1530/endoabs.81.EP1030

Tomasović M, Sinik M, Joksimović B, Lacković M, Samardžić V, Vujović M, Gluvić Z, Obradović M, Zafirović S, Isenović ER. Hand and foot skin changes resembling PTU-induced vasculitis in a young male with diffuse toxic goiter- a case report. in Endocrine Abstracts. 2022;.
doi:10.1530/endoabs.81.EP1030 .

Tomasović, Martina, Sinik, Marija, Joksimović, Bojan, Lacković, Milena, Samardžić, Vladimir, Vujović, Marina, Gluvić, Zoran, Obradović, Milan, Zafirović, Sonja, Isenović, Esma R., "Hand and foot skin changes resembling PTU-induced vasculitis in a young male with diffuse toxic goiter- a case report" in Endocrine Abstracts (2022),
https://doi.org/10.1530/endoabs.81.EP1030 . .

TY  - CONF
AU  - Soskić, Sanja
AU  - Obradović, Milan
AU  - Zafirović, Sonja
AU  - Ilinčić, B.
AU  - Čabarkapa, V.
AU  - Stokić, Edita
AU  - Isenović, Esma R.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12033
AB  - Rad je koncipiran, dizajniran i urađen u Laborastoriji za radiobiologiju i molekularnu genetiku Instituta za nuklearne nauke “Vinča” – Institutom od nacionalnog značaja – Univerziteta u Beogradu u saradnji sa Medicinskim fakultetom Univerziteta u Novom Sadu i Univerzitetskim Kliničkim CentromVojvodine. Uvod i cilj. Reaktivne vrste kiseonika (ROS) imaju mogućnost da reaguju sa biomolekulima ćelija i telesnih tečnosti i da ih menjaju. Oksidativni stres (OxS) nastaje u ćelijskim sistemima u uslovima narušene ravnoteže između stepena produkcije i otklanjanja visokoreaktivnih molekula, odnosno, kada produkcija ROS prevazilazi antioksidativne kapacitete datih sistema. Jedan od parametara OxS je 4-hidroksi 2-nonelan (4-HNE), čija koncentracija predstavlja stepen lipidne peroksidacije. Antioksidativni zaštitni sistem (AOS) nastao je tokom procesa evolucije u aerobnim uslovima kao odgovor na toksično delovanje kiseonika. Postoji više nivoa AOS, kao što su enzimski antioksidanti i neenzimski antioksidanti. Među najznačajnijim komponentama enzimskog AOS su superoksid dismutaza (SOD), katalaza (CAT), glutation peroksidaza (GPx) glutation reduktaza (GR). Cilj rada je bio utvrđivanje promena koncentracije parametra OxS i aktivnosti enzima AOS kod gojaznih ispitanika u odnosu na normalno uhranjene ispitanike. Metode. U studiju je bilo uključeno 67 osoba oba pola, od čega 36 normalno uhranjenih osoba (kontrole) i 31 gojazna osoba. Ukupni antioksidativni status (TAS) test je meren primenom Randox TAS kita sa Troloksom kao ekvivalentnim standardom na novoj generaciji Daytona (RX) automatskom hemijskom analizatoru prema uputstvu Randox Co. Za određivanje aktivnosti SOD, GPx i GR u serumu ispitanika korišćene su kolorimetrijske metode i komercijalno dostupni Randox kitovi (Randox Labs, Crumlin, UK). Za određivanje aktivnosti CAT i za određivanje koncentracije 4-HNE u serumu ispitanika korišćeni su komercijalno dostupni OxiSelect kitovi (Cell Biolabs, Inc., San Diego, USA). Rezultati. Dobijeni rezultati pokazuju da je koncentracija 4-HNE kod gojaznih osoba bila statistički značajno viša za 36% (p<0,001) u odnosu na nivo 4-HNE merenog kod kontrola. Nivo TAS kod gojaznih ispitanika bio je statistički značajno smanjen (p<0,001) u poređenju sa vrednostima za TAS kod kontrolnih ispitanika. Procenat smanjenja aktivnosti enzima AOS kod gojaznih osoba bio je 35% za SOD (p<0,001), 21% za GR (p<0,001) i 29% za GPx (p<0,001). Nije uočena statistički značajna promena za aktivnost CAT između dve ispitivane grupe. Zaključak. Dobijeni rezultati jasno pokazuju da stanje gojaznosti dovodi do smanjenja aktivnosti AOS, kao i povećanja koncentracije markera lipidne peroksidacije. Takođe, dobijeni rezultati sugerišu da bi određivanje enzima AOS i markera lipidne peroksidacije mogli biti jedni od biomarkera predikcije nastanka gojaznosti.
C3  - KES2022 : 8. Kongres endokrinologa Srbije sa međunarodnim učešćem : Program i zbornik sažetaka
T1  - Parametar oksidativnog stresa i enzimi antioksidativne zaštite kod gojaznih osoba u Srbiji
SP  - 98
EP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12033
ER  - 

@conference{
author = "Soskić, Sanja and Obradović, Milan and Zafirović, Sonja and Ilinčić, B. and Čabarkapa, V. and Stokić, Edita and Isenović, Esma R.",
year = "2022",
abstract = "Rad je koncipiran, dizajniran i urađen u Laborastoriji za radiobiologiju i molekularnu genetiku Instituta za nuklearne nauke “Vinča” – Institutom od nacionalnog značaja – Univerziteta u Beogradu u saradnji sa Medicinskim fakultetom Univerziteta u Novom Sadu i Univerzitetskim Kliničkim CentromVojvodine. Uvod i cilj. Reaktivne vrste kiseonika (ROS) imaju mogućnost da reaguju sa biomolekulima ćelija i telesnih tečnosti i da ih menjaju. Oksidativni stres (OxS) nastaje u ćelijskim sistemima u uslovima narušene ravnoteže između stepena produkcije i otklanjanja visokoreaktivnih molekula, odnosno, kada produkcija ROS prevazilazi antioksidativne kapacitete datih sistema. Jedan od parametara OxS je 4-hidroksi 2-nonelan (4-HNE), čija koncentracija predstavlja stepen lipidne peroksidacije. Antioksidativni zaštitni sistem (AOS) nastao je tokom procesa evolucije u aerobnim uslovima kao odgovor na toksično delovanje kiseonika. Postoji više nivoa AOS, kao što su enzimski antioksidanti i neenzimski antioksidanti. Među najznačajnijim komponentama enzimskog AOS su superoksid dismutaza (SOD), katalaza (CAT), glutation peroksidaza (GPx) glutation reduktaza (GR). Cilj rada je bio utvrđivanje promena koncentracije parametra OxS i aktivnosti enzima AOS kod gojaznih ispitanika u odnosu na normalno uhranjene ispitanike. Metode. U studiju je bilo uključeno 67 osoba oba pola, od čega 36 normalno uhranjenih osoba (kontrole) i 31 gojazna osoba. Ukupni antioksidativni status (TAS) test je meren primenom Randox TAS kita sa Troloksom kao ekvivalentnim standardom na novoj generaciji Daytona (RX) automatskom hemijskom analizatoru prema uputstvu Randox Co. Za određivanje aktivnosti SOD, GPx i GR u serumu ispitanika korišćene su kolorimetrijske metode i komercijalno dostupni Randox kitovi (Randox Labs, Crumlin, UK). Za određivanje aktivnosti CAT i za određivanje koncentracije 4-HNE u serumu ispitanika korišćeni su komercijalno dostupni OxiSelect kitovi (Cell Biolabs, Inc., San Diego, USA). Rezultati. Dobijeni rezultati pokazuju da je koncentracija 4-HNE kod gojaznih osoba bila statistički značajno viša za 36% (p<0,001) u odnosu na nivo 4-HNE merenog kod kontrola. Nivo TAS kod gojaznih ispitanika bio je statistički značajno smanjen (p<0,001) u poređenju sa vrednostima za TAS kod kontrolnih ispitanika. Procenat smanjenja aktivnosti enzima AOS kod gojaznih osoba bio je 35% za SOD (p<0,001), 21% za GR (p<0,001) i 29% za GPx (p<0,001). Nije uočena statistički značajna promena za aktivnost CAT između dve ispitivane grupe. Zaključak. Dobijeni rezultati jasno pokazuju da stanje gojaznosti dovodi do smanjenja aktivnosti AOS, kao i povećanja koncentracije markera lipidne peroksidacije. Takođe, dobijeni rezultati sugerišu da bi određivanje enzima AOS i markera lipidne peroksidacije mogli biti jedni od biomarkera predikcije nastanka gojaznosti.",
journal = "KES2022 : 8. Kongres endokrinologa Srbije sa međunarodnim učešćem : Program i zbornik sažetaka",
title = "Parametar oksidativnog stresa i enzimi antioksidativne zaštite kod gojaznih osoba u Srbiji",
pages = "98-98",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12033"
}

Soskić, S., Obradović, M., Zafirović, S., Ilinčić, B., Čabarkapa, V., Stokić, E.,& Isenović, E. R.. (2022). Parametar oksidativnog stresa i enzimi antioksidativne zaštite kod gojaznih osoba u Srbiji. in KES2022 : 8. Kongres endokrinologa Srbije sa međunarodnim učešćem : Program i zbornik sažetaka, 98-98.
https://hdl.handle.net/21.15107/rcub_vinar_12033

Soskić S, Obradović M, Zafirović S, Ilinčić B, Čabarkapa V, Stokić E, Isenović ER. Parametar oksidativnog stresa i enzimi antioksidativne zaštite kod gojaznih osoba u Srbiji. in KES2022 : 8. Kongres endokrinologa Srbije sa međunarodnim učešćem : Program i zbornik sažetaka. 2022;:98-98.
https://hdl.handle.net/21.15107/rcub_vinar_12033 .

Soskić, Sanja, Obradović, Milan, Zafirović, Sonja, Ilinčić, B., Čabarkapa, V., Stokić, Edita, Isenović, Esma R., "Parametar oksidativnog stresa i enzimi antioksidativne zaštite kod gojaznih osoba u Srbiji" in KES2022 : 8. Kongres endokrinologa Srbije sa međunarodnim učešćem : Program i zbornik sažetaka (2022):98-98,
https://hdl.handle.net/21.15107/rcub_vinar_12033 .

TY  - CONF
AU  - Tomasović, M.
AU  - Šinik, M.
AU  - Joksimović, Bojan
AU  - Lačković, M.
AU  - Samardžić, Vladimir
AU  - Gluvić, Zoran
AU  - Vujović, M.
AU  - Zafirović, Sonja
AU  - Mačvanin, Mirjana
AU  - Isenović, Esma R.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12034
AB  - Uvod i cilj. Hipotiroza je često endokrinološko oboljenje, sa širokim spektrom kliničke prezentacije: od asimptomatske do multiorganske, koja prognostički može biti ozbiljna. Atipične prezentacije hipotiroze retko mogu biti i inicijalne, poput perikardnog izliva (PI). Prikazujemo bolesnika sa novodijagnostikovanom primarnom hipotirozom, prezentovanom simptomima i znacima srčane insuficijencije, sa ehokardiografski detektovanim PI. Metode. Bolesnik je obrađen klinički, elektrokardiografski, laboratorijski i ultrasonografski (štitasta žlezda i srce). Rezultati. Muškarac star 47 godina se javlja u hitnu internističku ambulantu zbog otoka nogu, lica i zamaranja, trajanja oko godinu dana. Anamnestički bez poznatih komorbiditeta. Objektivno se kod hipometaboličnog bolesnika sa JVP 1+, bez prisustva Bekove trijade, detektuju pretibijalni edemi, bez drugog patološkog nalaza po sistemima. TA 110/70 mmHg, P 56/min. EKG: sinusni ritam, frekvence oko 55/min, niže voltaže, bez ST i T promena. U laboratorijskim analizama beleže se blaga normocitna anemija, HLP 2b i povišene vrednosti CK i transaminaza. TFT ukazuju na autoimunsku primarnu hipotirozu (TSH 35, FT4 <1.93, Anti TPO 234). Ehokardiografski nalaz ukazuje na cirkularni PI, bez znakova preteće tamponade, dok ultrazvuk štitaste žlezde odgovara hroničnom tiroiditisu. Vrednosti tumorskih i sistemskih autoimunskih markera su bez odstupanja. Započinje se postepenom supstitucijom levotiroksinom. Kontrolna ehokardiografska studija (pet nedelja nakon inicijalne) ukazuje na smanjenje PI, dok se laboratorijski registruje pad nivoa TSH (6.35). Zaključak. PI je retka inicijalna prezentacija hipotiroze, koja ako se prepozna blagovremeno, sprečava razvoj ozbiljnog kardiovaskularnog morbiditeta. Potrebno je, posebno kod mlađih bolesnika, razmotriti postojanje pridruženih uzroka PI (SBVT, maligne i infektivne bolesti), koji se klinički mogu prezentovati mitigirano.
C3  - KES2022 : 8. Kongres endokrinologa Srbije sa međunarodnim učešćem : Program i zbornik sažetaka
T1  - Perikardni izliv kao inicijalna prezentacija novodijagnostikovane primarne hipotiroze – prikaz slučaja
SP  - 134
EP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12034
ER  - 

@conference{
author = "Tomasović, M. and Šinik, M. and Joksimović, Bojan and Lačković, M. and Samardžić, Vladimir and Gluvić, Zoran and Vujović, M. and Zafirović, Sonja and Mačvanin, Mirjana and Isenović, Esma R.",
year = "2022",
abstract = "Uvod i cilj. Hipotiroza je često endokrinološko oboljenje, sa širokim spektrom kliničke prezentacije: od asimptomatske do multiorganske, koja prognostički može biti ozbiljna. Atipične prezentacije hipotiroze retko mogu biti i inicijalne, poput perikardnog izliva (PI). Prikazujemo bolesnika sa novodijagnostikovanom primarnom hipotirozom, prezentovanom simptomima i znacima srčane insuficijencije, sa ehokardiografski detektovanim PI. Metode. Bolesnik je obrađen klinički, elektrokardiografski, laboratorijski i ultrasonografski (štitasta žlezda i srce). Rezultati. Muškarac star 47 godina se javlja u hitnu internističku ambulantu zbog otoka nogu, lica i zamaranja, trajanja oko godinu dana. Anamnestički bez poznatih komorbiditeta. Objektivno se kod hipometaboličnog bolesnika sa JVP 1+, bez prisustva Bekove trijade, detektuju pretibijalni edemi, bez drugog patološkog nalaza po sistemima. TA 110/70 mmHg, P 56/min. EKG: sinusni ritam, frekvence oko 55/min, niže voltaže, bez ST i T promena. U laboratorijskim analizama beleže se blaga normocitna anemija, HLP 2b i povišene vrednosti CK i transaminaza. TFT ukazuju na autoimunsku primarnu hipotirozu (TSH 35, FT4 <1.93, Anti TPO 234). Ehokardiografski nalaz ukazuje na cirkularni PI, bez znakova preteće tamponade, dok ultrazvuk štitaste žlezde odgovara hroničnom tiroiditisu. Vrednosti tumorskih i sistemskih autoimunskih markera su bez odstupanja. Započinje se postepenom supstitucijom levotiroksinom. Kontrolna ehokardiografska studija (pet nedelja nakon inicijalne) ukazuje na smanjenje PI, dok se laboratorijski registruje pad nivoa TSH (6.35). Zaključak. PI je retka inicijalna prezentacija hipotiroze, koja ako se prepozna blagovremeno, sprečava razvoj ozbiljnog kardiovaskularnog morbiditeta. Potrebno je, posebno kod mlađih bolesnika, razmotriti postojanje pridruženih uzroka PI (SBVT, maligne i infektivne bolesti), koji se klinički mogu prezentovati mitigirano.",
journal = "KES2022 : 8. Kongres endokrinologa Srbije sa međunarodnim učešćem : Program i zbornik sažetaka",
title = "Perikardni izliv kao inicijalna prezentacija novodijagnostikovane primarne hipotiroze – prikaz slučaja",
pages = "134-134",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12034"
}

Tomasović, M., Šinik, M., Joksimović, B., Lačković, M., Samardžić, V., Gluvić, Z., Vujović, M., Zafirović, S., Mačvanin, M.,& Isenović, E. R.. (2022). Perikardni izliv kao inicijalna prezentacija novodijagnostikovane primarne hipotiroze – prikaz slučaja. in KES2022 : 8. Kongres endokrinologa Srbije sa međunarodnim učešćem : Program i zbornik sažetaka, 134-134.
https://hdl.handle.net/21.15107/rcub_vinar_12034

Tomasović M, Šinik M, Joksimović B, Lačković M, Samardžić V, Gluvić Z, Vujović M, Zafirović S, Mačvanin M, Isenović ER. Perikardni izliv kao inicijalna prezentacija novodijagnostikovane primarne hipotiroze – prikaz slučaja. in KES2022 : 8. Kongres endokrinologa Srbije sa međunarodnim učešćem : Program i zbornik sažetaka. 2022;:134-134.
https://hdl.handle.net/21.15107/rcub_vinar_12034 .

Tomasović, M., Šinik, M., Joksimović, Bojan, Lačković, M., Samardžić, Vladimir, Gluvić, Zoran, Vujović, M., Zafirović, Sonja, Mačvanin, Mirjana, Isenović, Esma R., "Perikardni izliv kao inicijalna prezentacija novodijagnostikovane primarne hipotiroze – prikaz slučaja" in KES2022 : 8. Kongres endokrinologa Srbije sa međunarodnim učešćem : Program i zbornik sažetaka (2022):134-134,
https://hdl.handle.net/21.15107/rcub_vinar_12034 .

TY  - CONF
AU  - Gluvić, Zoran
AU  - Lačković, Milena
AU  - Samardžić, Vladimir
AU  - Tomašević, Ratko
AU  - Pavlović, Aleksandar
AU  - Obradović, Milan
AU  - Zafirović, Sonja
AU  - Mladenović, Violeta
AU  - Radenković, Saša
AU  - Isenović, Esma R.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12035
C3  - 4. Srpski kongres o menopauzi i involutivnom hipoandrogenizmu : Knjiga apstrakata
T1  - Nealkoholna masna bolest jetre: klinički multidisciplinarni pristup- institucionalna adaptacija postojećim Vodičima kliničke prakse
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12035
ER  - 

@conference{
author = "Gluvić, Zoran and Lačković, Milena and Samardžić, Vladimir and Tomašević, Ratko and Pavlović, Aleksandar and Obradović, Milan and Zafirović, Sonja and Mladenović, Violeta and Radenković, Saša and Isenović, Esma R.",
year = "2022",
journal = "4. Srpski kongres o menopauzi i involutivnom hipoandrogenizmu : Knjiga apstrakata",
title = "Nealkoholna masna bolest jetre: klinički multidisciplinarni pristup- institucionalna adaptacija postojećim Vodičima kliničke prakse",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12035"
}

Gluvić, Z., Lačković, M., Samardžić, V., Tomašević, R., Pavlović, A., Obradović, M., Zafirović, S., Mladenović, V., Radenković, S.,& Isenović, E. R.. (2022). Nealkoholna masna bolest jetre: klinički multidisciplinarni pristup- institucionalna adaptacija postojećim Vodičima kliničke prakse. in 4. Srpski kongres o menopauzi i involutivnom hipoandrogenizmu : Knjiga apstrakata.
https://hdl.handle.net/21.15107/rcub_vinar_12035

Gluvić Z, Lačković M, Samardžić V, Tomašević R, Pavlović A, Obradović M, Zafirović S, Mladenović V, Radenković S, Isenović ER. Nealkoholna masna bolest jetre: klinički multidisciplinarni pristup- institucionalna adaptacija postojećim Vodičima kliničke prakse. in 4. Srpski kongres o menopauzi i involutivnom hipoandrogenizmu : Knjiga apstrakata. 2022;.
https://hdl.handle.net/21.15107/rcub_vinar_12035 .

Gluvić, Zoran, Lačković, Milena, Samardžić, Vladimir, Tomašević, Ratko, Pavlović, Aleksandar, Obradović, Milan, Zafirović, Sonja, Mladenović, Violeta, Radenković, Saša, Isenović, Esma R., "Nealkoholna masna bolest jetre: klinički multidisciplinarni pristup- institucionalna adaptacija postojećim Vodičima kliničke prakse" in 4. Srpski kongres o menopauzi i involutivnom hipoandrogenizmu : Knjiga apstrakata (2022),
https://hdl.handle.net/21.15107/rcub_vinar_12035 .

TY  - CONF
AU  - Samardžić, Vladimir
AU  - Lačković, Milena
AU  - Joksimović, Bojan
AU  - Šinik, M.
AU  - Miladinović, M.
AU  - Zorić, G.
AU  - Obradović, Milan
AU  - Zafirović, Sonja
AU  - Isenović, Esma R.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12036
AB  - Definisanje malignog potencijala tiroidnih nodusa (TN) je najznačajnija odrednica u njihovoj evaluaciji. Primena imidžing metoda, kao i biohemijskih, citoloških i molekularno bioloških alata doprinosi razlikovanju benignih od malignih TN i tako delimično smanjuje broj nepotrebno tiroidektomsanih pacijenata. Jasno je da idealni biomarker ili metoda za definisanje prirode TN ne postoji, ali smo svedoci nastojanja kliničara da detektuju biomarker ili biomarkere koji bi samostalno ili u kombinaciji sa drugim alatima omogućili još kvalitetniju regrutaciju ispitanika kojima je tiroidektomija zaista potrebna. Studija Samardžića i aut. je analizirala biomarkere u ispirku bioptata TN i pokazala da nivo tiroglobulina u ispirku (TGw) pozitivno koreliše sa Bethesda kategorijom citološkog nalaza bioptata TN, dok nivo NOw pozitivno koreliše sa EU-TIRADS kategorijama. Tako se kod četvoro od petoro tiroidektomisanih ispitanika registrovala pripadnost EU-TIRADS kategorijama 4 i 5. Potencijal NOw i TGw kao pomoćnog alata za preciziranje prirode TN je nedvosmislen, te u budućim kliničkim studijama treba analizirati njihovu pojedinačnu i pridruženu prediktivnost u definisanju malignih TN na većoj populaciji ispitanika.
PB  - Beograd : Srpsko Tiroidno Društvo
C3  - 7. Srpski kongres o štitastoj žlezdi : Finalni program i Zbornik sažetaka
T1  - Azot-monoksid- biomarker u dijagnostici tiroidnih nodusa
SP  - 19
EP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12036
ER  - 

@conference{
author = "Samardžić, Vladimir and Lačković, Milena and Joksimović, Bojan and Šinik, M. and Miladinović, M. and Zorić, G. and Obradović, Milan and Zafirović, Sonja and Isenović, Esma R.",
year = "2022",
abstract = "Definisanje malignog potencijala tiroidnih nodusa (TN) je najznačajnija odrednica u njihovoj evaluaciji. Primena imidžing metoda, kao i biohemijskih, citoloških i molekularno bioloških alata doprinosi razlikovanju benignih od malignih TN i tako delimično smanjuje broj nepotrebno tiroidektomsanih pacijenata. Jasno je da idealni biomarker ili metoda za definisanje prirode TN ne postoji, ali smo svedoci nastojanja kliničara da detektuju biomarker ili biomarkere koji bi samostalno ili u kombinaciji sa drugim alatima omogućili još kvalitetniju regrutaciju ispitanika kojima je tiroidektomija zaista potrebna. Studija Samardžića i aut. je analizirala biomarkere u ispirku bioptata TN i pokazala da nivo tiroglobulina u ispirku (TGw) pozitivno koreliše sa Bethesda kategorijom citološkog nalaza bioptata TN, dok nivo NOw pozitivno koreliše sa EU-TIRADS kategorijama. Tako se kod četvoro od petoro tiroidektomisanih ispitanika registrovala pripadnost EU-TIRADS kategorijama 4 i 5. Potencijal NOw i TGw kao pomoćnog alata za preciziranje prirode TN je nedvosmislen, te u budućim kliničkim studijama treba analizirati njihovu pojedinačnu i pridruženu prediktivnost u definisanju malignih TN na većoj populaciji ispitanika.",
publisher = "Beograd : Srpsko Tiroidno Društvo",
journal = "7. Srpski kongres o štitastoj žlezdi : Finalni program i Zbornik sažetaka",
title = "Azot-monoksid- biomarker u dijagnostici tiroidnih nodusa",
pages = "19-19",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12036"
}

Samardžić, V., Lačković, M., Joksimović, B., Šinik, M., Miladinović, M., Zorić, G., Obradović, M., Zafirović, S.,& Isenović, E. R.. (2022). Azot-monoksid- biomarker u dijagnostici tiroidnih nodusa. in 7. Srpski kongres o štitastoj žlezdi : Finalni program i Zbornik sažetaka
Beograd : Srpsko Tiroidno Društvo., 19-19.
https://hdl.handle.net/21.15107/rcub_vinar_12036

Samardžić V, Lačković M, Joksimović B, Šinik M, Miladinović M, Zorić G, Obradović M, Zafirović S, Isenović ER. Azot-monoksid- biomarker u dijagnostici tiroidnih nodusa. in 7. Srpski kongres o štitastoj žlezdi : Finalni program i Zbornik sažetaka. 2022;:19-19.
https://hdl.handle.net/21.15107/rcub_vinar_12036 .

Samardžić, Vladimir, Lačković, Milena, Joksimović, Bojan, Šinik, M., Miladinović, M., Zorić, G., Obradović, Milan, Zafirović, Sonja, Isenović, Esma R., "Azot-monoksid- biomarker u dijagnostici tiroidnih nodusa" in 7. Srpski kongres o štitastoj žlezdi : Finalni program i Zbornik sažetaka (2022):19-19,
https://hdl.handle.net/21.15107/rcub_vinar_12036 .

TY  - JOUR
AU  - Stanimirović, Julijana
AU  - Radovanović, Jelena
AU  - Banjac, Katarina
AU  - Obradović, Milan M.
AU  - Essack, Magbubah
AU  - Zafirović, Sonja
AU  - Gluvić, Zoran
AU  - Gojobori, Takashi
AU  - Isenović, Esma R.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10285
AB  - Even though type 2 diabetes mellitus (T2DM) represents a worldwide chronic health issue that affects about 462 million people, specific underlying determinants of insulin resistance (IR) and impaired insulin secretion are still unknown. There is growing evidence that chronic subclinical inflammation is a triggering factor in the origin of T2DM. Increased C-reactive protein (CRP) levels have been linked to excess body weight since adipocytes produce tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6), which are pivotal factors for CRP stimulation. Furthermore, it is known that hepatocytes produce relatively low rates of CRP in physiological conditions compared to T2DM patients, in which elevated levels of inflammatory markers are reported, including CRP. CRP also participates in endothelial dysfunction, the production of vasodilators, and vascular remodeling, and increased CRP level is closely associated with vascular system pathology and metabolic syndrome. In addition, insulin-based therapies may alter CRP levels in T2DM. Therefore, determining and clarifying the underlying CRP mechanism of T2DM is imperative for novel preventive and diagnostic procedures. Overall, CRP is one of the possible targets for T2DM progression and understanding the connection between insulin and inflammation may be helpful in clinical treatment and prevention approaches.
T2  - Mediators of Inflammation
T1  - Role of C-Reactive Protein in Diabetic Inflammation
VL  - 2022
SP  - e3706508
DO  - 10.1155/2022/3706508
ER  - 

@article{
author = "Stanimirović, Julijana and Radovanović, Jelena and Banjac, Katarina and Obradović, Milan M. and Essack, Magbubah and Zafirović, Sonja and Gluvić, Zoran and Gojobori, Takashi and Isenović, Esma R.",
year = "2022",
abstract = "Even though type 2 diabetes mellitus (T2DM) represents a worldwide chronic health issue that affects about 462 million people, specific underlying determinants of insulin resistance (IR) and impaired insulin secretion are still unknown. There is growing evidence that chronic subclinical inflammation is a triggering factor in the origin of T2DM. Increased C-reactive protein (CRP) levels have been linked to excess body weight since adipocytes produce tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6), which are pivotal factors for CRP stimulation. Furthermore, it is known that hepatocytes produce relatively low rates of CRP in physiological conditions compared to T2DM patients, in which elevated levels of inflammatory markers are reported, including CRP. CRP also participates in endothelial dysfunction, the production of vasodilators, and vascular remodeling, and increased CRP level is closely associated with vascular system pathology and metabolic syndrome. In addition, insulin-based therapies may alter CRP levels in T2DM. Therefore, determining and clarifying the underlying CRP mechanism of T2DM is imperative for novel preventive and diagnostic procedures. Overall, CRP is one of the possible targets for T2DM progression and understanding the connection between insulin and inflammation may be helpful in clinical treatment and prevention approaches.",
journal = "Mediators of Inflammation",
title = "Role of C-Reactive Protein in Diabetic Inflammation",
volume = "2022",
pages = "e3706508",
doi = "10.1155/2022/3706508"
}

Stanimirović, J., Radovanović, J., Banjac, K., Obradović, M. M., Essack, M., Zafirović, S., Gluvić, Z., Gojobori, T.,& Isenović, E. R.. (2022). Role of C-Reactive Protein in Diabetic Inflammation. in Mediators of Inflammation, 2022, e3706508.
https://doi.org/10.1155/2022/3706508

Stanimirović J, Radovanović J, Banjac K, Obradović MM, Essack M, Zafirović S, Gluvić Z, Gojobori T, Isenović ER. Role of C-Reactive Protein in Diabetic Inflammation. in Mediators of Inflammation. 2022;2022:e3706508.
doi:10.1155/2022/3706508 .

Stanimirović, Julijana, Radovanović, Jelena, Banjac, Katarina, Obradović, Milan M., Essack, Magbubah, Zafirović, Sonja, Gluvić, Zoran, Gojobori, Takashi, Isenović, Esma R., "Role of C-Reactive Protein in Diabetic Inflammation" in Mediators of Inflammation, 2022 (2022):e3706508,
https://doi.org/10.1155/2022/3706508 . .

TY  - JOUR
AU  - Gluvić, Zoran
AU  - Obradović, Milan M.
AU  - Sudar-Milovanović, Emina
AU  - Zafirović, Sonja
AU  - Radak, Đorđe J.
AU  - Essack, Magbubah
AU  - Bajić, Vladimir B.
AU  - Gojobori, Takashi
AU  - Isenović, Esma R.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8474
AB  - Hypothyroidism is a common endocrine disorder that predominantly occurs in females. It is associated with an increased risk of cardiovascular diseases (CVD), but the molecular mechanism is not known. Disturbance in lipid metabolism, the regulation of oxidative stress, and inflammation characterize the progression of subclinical hypothyroidism. The initiation and progression of endothelial dysfunction also exhibit these changes, which is the initial step in developing CVD. Animal and human studies highlight the critical role of nitric oxide (NO) as a reliable biomarker for cardiovascular risk in subclinical and clinical hypothyroidism. In this review, we summarize the recent literature findings associated with NO production by the thyroid hormones in both physiological and pathophysiological conditions. We also discuss the levothyroxine treatment effect on serum NO levels in hypothyroid patients. © 2020 The Authors
T2  - Biomedicine and Pharmacotherapy
T1  - Regulation of nitric oxide production in hypothyroidism
VL  - 124
SP  - 109881
DO  - 10.1016/j.biopha.2020.109881
ER  - 

@article{
author = "Gluvić, Zoran and Obradović, Milan M. and Sudar-Milovanović, Emina and Zafirović, Sonja and Radak, Đorđe J. and Essack, Magbubah and Bajić, Vladimir B. and Gojobori, Takashi and Isenović, Esma R.",
year = "2020",
abstract = "Hypothyroidism is a common endocrine disorder that predominantly occurs in females. It is associated with an increased risk of cardiovascular diseases (CVD), but the molecular mechanism is not known. Disturbance in lipid metabolism, the regulation of oxidative stress, and inflammation characterize the progression of subclinical hypothyroidism. The initiation and progression of endothelial dysfunction also exhibit these changes, which is the initial step in developing CVD. Animal and human studies highlight the critical role of nitric oxide (NO) as a reliable biomarker for cardiovascular risk in subclinical and clinical hypothyroidism. In this review, we summarize the recent literature findings associated with NO production by the thyroid hormones in both physiological and pathophysiological conditions. We also discuss the levothyroxine treatment effect on serum NO levels in hypothyroid patients. © 2020 The Authors",
journal = "Biomedicine and Pharmacotherapy",
title = "Regulation of nitric oxide production in hypothyroidism",
volume = "124",
pages = "109881",
doi = "10.1016/j.biopha.2020.109881"
}

Gluvić, Z., Obradović, M. M., Sudar-Milovanović, E., Zafirović, S., Radak, Đ. J., Essack, M., Bajić, V. B., Gojobori, T.,& Isenović, E. R.. (2020). Regulation of nitric oxide production in hypothyroidism. in Biomedicine and Pharmacotherapy, 124, 109881.
https://doi.org/10.1016/j.biopha.2020.109881

Gluvić Z, Obradović MM, Sudar-Milovanović E, Zafirović S, Radak ĐJ, Essack M, Bajić VB, Gojobori T, Isenović ER. Regulation of nitric oxide production in hypothyroidism. in Biomedicine and Pharmacotherapy. 2020;124:109881.
doi:10.1016/j.biopha.2020.109881 .

Gluvić, Zoran, Obradović, Milan M., Sudar-Milovanović, Emina, Zafirović, Sonja, Radak, Đorđe J., Essack, Magbubah, Bajić, Vladimir B., Gojobori, Takashi, Isenović, Esma R., "Regulation of nitric oxide production in hypothyroidism" in Biomedicine and Pharmacotherapy, 124 (2020):109881,
https://doi.org/10.1016/j.biopha.2020.109881 . .

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Zafirović, Sonja
AU  - Essack, Magbubah
AU  - Dimitrov, Jelena
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Radak, Đorđe J.
AU  - Bajić, Vladimir B.
AU  - Isenović, Esma R.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8487
AB  - To remedy carotid artery stenosis and prevent stroke surgical intervention is commonly used, and the gold standard being carotid endarterectomy (CEA). During CEA cerebrovascular hemoglobin oxygen saturation decreases and when this decrease reaches critical levels it leads to cerebral hypoxia that causes neuronal damage. One of the proposed mechanism that affects changes during CEA and contribute to acute brain ischemia (ABI) is oxidative stress. The increased production of reactive oxygen species and reactive nitrogen species during ABI may cause an unregulated inflammatory response and further lead to structural and functional injury of neurons. Antioxidant activity are involved in the protection against neuronal damage after cerebral ischemia. We hypothesized that neuronal injury and poor outcomes in patients undergoing CEA may be results of oxidative stress that disturbed function of antioxidant enzymes and contributed to the DNA damage in lymphocytes. © 2019 The Authors
PB  - Churchill Livingstone
T2  - Medical Hypotheses
T1  - Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy
VL  - 134
SP  - 109419
DO  - 10.1016/j.mehy.2019.109419
ER  - 

@article{
author = "Obradović, Milan M. and Zafirović, Sonja and Essack, Magbubah and Dimitrov, Jelena and Živković, Lada and Spremo-Potparević, Biljana and Radak, Đorđe J. and Bajić, Vladimir B. and Isenović, Esma R.",
year = "2020",
abstract = "To remedy carotid artery stenosis and prevent stroke surgical intervention is commonly used, and the gold standard being carotid endarterectomy (CEA). During CEA cerebrovascular hemoglobin oxygen saturation decreases and when this decrease reaches critical levels it leads to cerebral hypoxia that causes neuronal damage. One of the proposed mechanism that affects changes during CEA and contribute to acute brain ischemia (ABI) is oxidative stress. The increased production of reactive oxygen species and reactive nitrogen species during ABI may cause an unregulated inflammatory response and further lead to structural and functional injury of neurons. Antioxidant activity are involved in the protection against neuronal damage after cerebral ischemia. We hypothesized that neuronal injury and poor outcomes in patients undergoing CEA may be results of oxidative stress that disturbed function of antioxidant enzymes and contributed to the DNA damage in lymphocytes. © 2019 The Authors",
publisher = "Churchill Livingstone",
journal = "Medical Hypotheses",
title = "Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy",
volume = "134",
pages = "109419",
doi = "10.1016/j.mehy.2019.109419"
}

Obradović, M. M., Zafirović, S., Essack, M., Dimitrov, J., Živković, L., Spremo-Potparević, B., Radak, Đ. J., Bajić, V. B.,& Isenović, E. R.. (2020). Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy. in Medical Hypotheses
Churchill Livingstone., 134, 109419.
https://doi.org/10.1016/j.mehy.2019.109419

Obradović MM, Zafirović S, Essack M, Dimitrov J, Živković L, Spremo-Potparević B, Radak ĐJ, Bajić VB, Isenović ER. Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy. in Medical Hypotheses. 2020;134:109419.
doi:10.1016/j.mehy.2019.109419 .

Obradović, Milan M., Zafirović, Sonja, Essack, Magbubah, Dimitrov, Jelena, Živković, Lada, Spremo-Potparević, Biljana, Radak, Đorđe J., Bajić, Vladimir B., Isenović, Esma R., "Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy" in Medical Hypotheses, 134 (2020):109419,
https://doi.org/10.1016/j.mehy.2019.109419 . .

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Essack, Magbubah
AU  - Živković, Lada
AU  - Stewart, Alan J.
AU  - Zafirović, Sonja
AU  - Bajić, Vladimir B.
AU  - Gojobori, Takashi
AU  - Isenović, Esma R.
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8825
AB  - Alzheimer’s disease (AD) is a neurodegenerative disease that affects millions of individuals worldwide and can occur relatively early or later in life. It is well known that genetic components, such as the amyloid precursor protein gene on chromosome 21, are fundamental in early-onset AD (EOAD). To date, however, only the apolipoprotein E4 (ApoE4) gene has been proved to be a genetic risk factor for late-onset AD (LOAD). In recent years, despite the hypothesis that many additional unidentified genes are likely to play a role in AD development, it is surprising that additional gene polymorphisms associated with LOAD have failed to come to light. In this review, we examine the role of X chromosome epigenetics and, based upon GWAS studies, the PCDHX11 gene. Furthermore, we explore other genetic risk factors of AD that involve X-chromosome epigenetics. © Copyright © 2020 Bajic, Essack, Zivkovic, Stewart, Zafirovic, Bajic, Gojobori, Isenovic and Spremo-Potparevic.
T2  - Frontiers in Genetics
T1  - The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”
VL  - 10
DO  - 10.3389/fgene.2019.01368
ER  - 

@article{
author = "Bajić, Vladan P. and Essack, Magbubah and Živković, Lada and Stewart, Alan J. and Zafirović, Sonja and Bajić, Vladimir B. and Gojobori, Takashi and Isenović, Esma R. and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Alzheimer’s disease (AD) is a neurodegenerative disease that affects millions of individuals worldwide and can occur relatively early or later in life. It is well known that genetic components, such as the amyloid precursor protein gene on chromosome 21, are fundamental in early-onset AD (EOAD). To date, however, only the apolipoprotein E4 (ApoE4) gene has been proved to be a genetic risk factor for late-onset AD (LOAD). In recent years, despite the hypothesis that many additional unidentified genes are likely to play a role in AD development, it is surprising that additional gene polymorphisms associated with LOAD have failed to come to light. In this review, we examine the role of X chromosome epigenetics and, based upon GWAS studies, the PCDHX11 gene. Furthermore, we explore other genetic risk factors of AD that involve X-chromosome epigenetics. © Copyright © 2020 Bajic, Essack, Zivkovic, Stewart, Zafirovic, Bajic, Gojobori, Isenovic and Spremo-Potparevic.",
journal = "Frontiers in Genetics",
title = "The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”",
volume = "10",
doi = "10.3389/fgene.2019.01368"
}

Bajić, V. P., Essack, M., Živković, L., Stewart, A. J., Zafirović, S., Bajić, V. B., Gojobori, T., Isenović, E. R.,& Spremo-Potparević, B.. (2020). The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”. in Frontiers in Genetics, 10.
https://doi.org/10.3389/fgene.2019.01368

Bajić VP, Essack M, Živković L, Stewart AJ, Zafirović S, Bajić VB, Gojobori T, Isenović ER, Spremo-Potparević B. The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”. in Frontiers in Genetics. 2020;10.
doi:10.3389/fgene.2019.01368 .

Bajić, Vladan P., Essack, Magbubah, Živković, Lada, Stewart, Alan J., Zafirović, Sonja, Bajić, Vladimir B., Gojobori, Takashi, Isenović, Esma R., Spremo-Potparević, Biljana, "The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”" in Frontiers in Genetics, 10 (2020),
https://doi.org/10.3389/fgene.2019.01368 . .

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Essack, Magbubah
AU  - Zafirović, Sonja
AU  - Sudar-Milovanović, Emina
AU  - Bajić, Vladan P.
AU  - Van Neste, Christophe
AU  - Trpković, Andreja
AU  - Stanimirović, Julijana
AU  - Bajić, Vladimir B.
AU  - Isenović, Esma R.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8486
AB  - Redox control is lost when the antioxidant defense system cannot remove abnormally high concentrations of signaling molecules, such as reactive oxygen species (ROS). Chronically elevated levels of ROS cause oxidative stress that may eventually lead to cancer and cardiovascular and neurodegenerative diseases. In this review, we focus on redox effects in the vascular system. We pay close attention to the subcompartments of the vascular system (endothelium, smooth muscle cell layer) and give an overview of how redox changes influence those different compartments. We also review the core aspects of redox biology, cardiovascular physiology, and pathophysiology. Moreover, the topic-specific knowledgebase DES-RedoxVasc was used to develop two case studies, one focused on endothelial cells and the other on the vascular smooth muscle cells, as a starting point to possibly extend our knowledge of redox control in vascular biology. © 2019 The Authors. BioFactors published by Wiley Periodicals, Inc. on behalf of International Union of Biochemistry and Molecular Biology.
T2  - BioFactors
T1  - Redox control of vascular biology
VL  - 46
IS  - 2
SP  - 246
EP  - 262
DO  - 10.1002/biof.1559
ER  - 

@article{
author = "Obradović, Milan M. and Essack, Magbubah and Zafirović, Sonja and Sudar-Milovanović, Emina and Bajić, Vladan P. and Van Neste, Christophe and Trpković, Andreja and Stanimirović, Julijana and Bajić, Vladimir B. and Isenović, Esma R.",
year = "2020",
abstract = "Redox control is lost when the antioxidant defense system cannot remove abnormally high concentrations of signaling molecules, such as reactive oxygen species (ROS). Chronically elevated levels of ROS cause oxidative stress that may eventually lead to cancer and cardiovascular and neurodegenerative diseases. In this review, we focus on redox effects in the vascular system. We pay close attention to the subcompartments of the vascular system (endothelium, smooth muscle cell layer) and give an overview of how redox changes influence those different compartments. We also review the core aspects of redox biology, cardiovascular physiology, and pathophysiology. Moreover, the topic-specific knowledgebase DES-RedoxVasc was used to develop two case studies, one focused on endothelial cells and the other on the vascular smooth muscle cells, as a starting point to possibly extend our knowledge of redox control in vascular biology. © 2019 The Authors. BioFactors published by Wiley Periodicals, Inc. on behalf of International Union of Biochemistry and Molecular Biology.",
journal = "BioFactors",
title = "Redox control of vascular biology",
volume = "46",
number = "2",
pages = "246-262",
doi = "10.1002/biof.1559"
}

Obradović, M. M., Essack, M., Zafirović, S., Sudar-Milovanović, E., Bajić, V. P., Van Neste, C., Trpković, A., Stanimirović, J., Bajić, V. B.,& Isenović, E. R.. (2020). Redox control of vascular biology. in BioFactors, 46(2), 246-262.
https://doi.org/10.1002/biof.1559

Obradović MM, Essack M, Zafirović S, Sudar-Milovanović E, Bajić VP, Van Neste C, Trpković A, Stanimirović J, Bajić VB, Isenović ER. Redox control of vascular biology. in BioFactors. 2020;46(2):246-262.
doi:10.1002/biof.1559 .

Obradović, Milan M., Essack, Magbubah, Zafirović, Sonja, Sudar-Milovanović, Emina, Bajić, Vladan P., Van Neste, Christophe, Trpković, Andreja, Stanimirović, Julijana, Bajić, Vladimir B., Isenović, Esma R., "Redox control of vascular biology" in BioFactors, 46, no. 2 (2020):246-262,
https://doi.org/10.1002/biof.1559 . .

TY  - JOUR
AU  - Essack, Magbubah
AU  - Salhi, Adil
AU  - Van Neste, Christophe
AU  - Raies, Arwa Bin
AU  - Tifratene, Faroug
AU  - Uludag, Mahmut
AU  - Hungler, Arnaud
AU  - Zarić, Božidarka
AU  - Zafirović, Sonja
AU  - Gojobori, Takashi
AU  - Isenović, Esma R.
AU  - Bajić, Vladan P.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8945
AB  - Normal cellular physiology and biochemical processes require undamaged RNA molecules. However, RNAs are frequently subjected to oxidative damage. Overproduction of reactive oxygen species (ROS) leads to RNA oxidation and disturbs redox (oxidation-reduction reaction) homeostasis. When oxidation damage affects RNA carrying protein-coding information, this may result in the synthesis of aberrant proteins as well as a lower efficiency of translation. Both of these, as well as imbalanced redox homeostasis, may lead to numerous human diseases. The number of studies on the effects of RNA oxidative damage in mammals is increasing by year due to the understanding that this oxidation fundamentally leads to numerous human diseases. To enable researchers in this field to explore information relevant to RNA oxidation and effects on human diseases, we developed DES-ROD, an online knowledgebase that contains processed information from 298,603 relevant documents that consist of PubMed abstracts and PubMed Central full-text articles. The system utilizes concepts/terms from 38 curated thematic dictionaries mapped to the analyzed documents. Researchers can explore enriched concepts, as well as enriched pairs of putatively associated concepts. In this way, one can explore mutual relationships between any combinations of two concepts from used dictionaries. Dictionaries cover a wide range of biomedical topics, such as human genes and proteins, pathways, Gene Ontology categories, mutations, noncoding RNAs, enzymes, toxins, metabolites, and diseases. This makes insights into different facets of the effects of RNA oxidation and the control of this process possible. The usefulness of the DES-ROD system is demonstrated by case studies on some known information, as well as potentially novel information involving RNA oxidation and diseases. DES-ROD is the first knowledgebase based on text and data mining that focused on the exploration of RNA oxidation and human diseases.
T2  - Oxidative Medicine and Cellular Longevity
T1  - DES-ROD: Exploring Literature to Develop New Links between RNA Oxidation and Human Diseases
VL  - 2020
SP  - 5904315
DO  - 10.1155/2020/5904315
ER  - 

@article{
author = "Essack, Magbubah and Salhi, Adil and Van Neste, Christophe and Raies, Arwa Bin and Tifratene, Faroug and Uludag, Mahmut and Hungler, Arnaud and Zarić, Božidarka and Zafirović, Sonja and Gojobori, Takashi and Isenović, Esma R. and Bajić, Vladan P.",
year = "2020",
abstract = "Normal cellular physiology and biochemical processes require undamaged RNA molecules. However, RNAs are frequently subjected to oxidative damage. Overproduction of reactive oxygen species (ROS) leads to RNA oxidation and disturbs redox (oxidation-reduction reaction) homeostasis. When oxidation damage affects RNA carrying protein-coding information, this may result in the synthesis of aberrant proteins as well as a lower efficiency of translation. Both of these, as well as imbalanced redox homeostasis, may lead to numerous human diseases. The number of studies on the effects of RNA oxidative damage in mammals is increasing by year due to the understanding that this oxidation fundamentally leads to numerous human diseases. To enable researchers in this field to explore information relevant to RNA oxidation and effects on human diseases, we developed DES-ROD, an online knowledgebase that contains processed information from 298,603 relevant documents that consist of PubMed abstracts and PubMed Central full-text articles. The system utilizes concepts/terms from 38 curated thematic dictionaries mapped to the analyzed documents. Researchers can explore enriched concepts, as well as enriched pairs of putatively associated concepts. In this way, one can explore mutual relationships between any combinations of two concepts from used dictionaries. Dictionaries cover a wide range of biomedical topics, such as human genes and proteins, pathways, Gene Ontology categories, mutations, noncoding RNAs, enzymes, toxins, metabolites, and diseases. This makes insights into different facets of the effects of RNA oxidation and the control of this process possible. The usefulness of the DES-ROD system is demonstrated by case studies on some known information, as well as potentially novel information involving RNA oxidation and diseases. DES-ROD is the first knowledgebase based on text and data mining that focused on the exploration of RNA oxidation and human diseases.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "DES-ROD: Exploring Literature to Develop New Links between RNA Oxidation and Human Diseases",
volume = "2020",
pages = "5904315",
doi = "10.1155/2020/5904315"
}

Essack, M., Salhi, A., Van Neste, C., Raies, A. B., Tifratene, F., Uludag, M., Hungler, A., Zarić, B., Zafirović, S., Gojobori, T., Isenović, E. R.,& Bajić, V. P.. (2020). DES-ROD: Exploring Literature to Develop New Links between RNA Oxidation and Human Diseases. in Oxidative Medicine and Cellular Longevity, 2020, 5904315.
https://doi.org/10.1155/2020/5904315

Essack M, Salhi A, Van Neste C, Raies AB, Tifratene F, Uludag M, Hungler A, Zarić B, Zafirović S, Gojobori T, Isenović ER, Bajić VP. DES-ROD: Exploring Literature to Develop New Links between RNA Oxidation and Human Diseases. in Oxidative Medicine and Cellular Longevity. 2020;2020:5904315.
doi:10.1155/2020/5904315 .

Essack, Magbubah, Salhi, Adil, Van Neste, Christophe, Raies, Arwa Bin, Tifratene, Faroug, Uludag, Mahmut, Hungler, Arnaud, Zarić, Božidarka, Zafirović, Sonja, Gojobori, Takashi, Isenović, Esma R., Bajić, Vladan P., "DES-ROD: Exploring Literature to Develop New Links between RNA Oxidation and Human Diseases" in Oxidative Medicine and Cellular Longevity, 2020 (2020):5904315,
https://doi.org/10.1155/2020/5904315 . .

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Van Neste, Christophe
AU  - Obradović, Milan M.
AU  - Zafirović, Sonja
AU  - Radak, Đorđe J.
AU  - Bajić, Vladimir B.
AU  - Essack, Magbubah
AU  - Isenović, Esma R.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8375
AB  - More people die from cardiovascular diseases (CVD) than from any other cause. Cardiovascular complications are thought to arise from enhanced levels of free radicals causing impaired “redox homeostasis,” which represents the interplay between oxidative stress (OS) and reductive stress (RS). In this review, we compile several experimental research findings that show sustained shifts towards OS will alter the homeostatic redox mechanism to cause cardiovascular complications, as well as findings that show a prolonged antioxidant state or RS can similarly lead to such cardiovascular complications. This experimental evidence is specifically focused on the role of glutathione, the most abundant antioxidant in the heart, in a redox homeostatic mechanism that has been shifted towards OS or RS. This may lead to impairment of cellular signaling mechanisms and elevated pools of proteotoxicity associated with cardiac dysfunction.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Glutathione “Redox Homeostasis” and Its Relation to Cardiovascular Disease
VL  - 2019
SP  - 5028181
DO  - 10.1155/2019/5028181
ER  - 

@article{
author = "Bajić, Vladan P. and Van Neste, Christophe and Obradović, Milan M. and Zafirović, Sonja and Radak, Đorđe J. and Bajić, Vladimir B. and Essack, Magbubah and Isenović, Esma R.",
year = "2019",
abstract = "More people die from cardiovascular diseases (CVD) than from any other cause. Cardiovascular complications are thought to arise from enhanced levels of free radicals causing impaired “redox homeostasis,” which represents the interplay between oxidative stress (OS) and reductive stress (RS). In this review, we compile several experimental research findings that show sustained shifts towards OS will alter the homeostatic redox mechanism to cause cardiovascular complications, as well as findings that show a prolonged antioxidant state or RS can similarly lead to such cardiovascular complications. This experimental evidence is specifically focused on the role of glutathione, the most abundant antioxidant in the heart, in a redox homeostatic mechanism that has been shifted towards OS or RS. This may lead to impairment of cellular signaling mechanisms and elevated pools of proteotoxicity associated with cardiac dysfunction.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Glutathione “Redox Homeostasis” and Its Relation to Cardiovascular Disease",
volume = "2019",
pages = "5028181",
doi = "10.1155/2019/5028181"
}

Bajić, V. P., Van Neste, C., Obradović, M. M., Zafirović, S., Radak, Đ. J., Bajić, V. B., Essack, M.,& Isenović, E. R.. (2019). Glutathione “Redox Homeostasis” and Its Relation to Cardiovascular Disease. in Oxidative Medicine and Cellular Longevity, 2019, 5028181.
https://doi.org/10.1155/2019/5028181

Bajić VP, Van Neste C, Obradović MM, Zafirović S, Radak ĐJ, Bajić VB, Essack M, Isenović ER. Glutathione “Redox Homeostasis” and Its Relation to Cardiovascular Disease. in Oxidative Medicine and Cellular Longevity. 2019;2019:5028181.
doi:10.1155/2019/5028181 .

Bajić, Vladan P., Van Neste, Christophe, Obradović, Milan M., Zafirović, Sonja, Radak, Đorđe J., Bajić, Vladimir B., Essack, Magbubah, Isenović, Esma R., "Glutathione “Redox Homeostasis” and Its Relation to Cardiovascular Disease" in Oxidative Medicine and Cellular Longevity, 2019 (2019):5028181,
https://doi.org/10.1155/2019/5028181 . .

TY  - JOUR
AU  - Zafirović, Sonja
AU  - Sudar-Milovanović, Emina
AU  - Obradović, Milan M.
AU  - Đorđević, Jelena D.
AU  - Jasnić, Nebojša
AU  - Labudović-Borović, Milica
AU  - Isenović, Esma R.
PY  - 2019
UR  - http://www.eurekaselect.com/159734/article
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8097
AB  - BACKGROUND: Oestradiol is an important regulatory factor with several positive effects on the cardiovascular (CV) system. We evaluated the molecular mechanism of the in vivo effects of oestradiol on the regulation of cardiac inducible nitric oxide (NO) synthase (iNOS) expression and activity. METHODS: Male Wistar rats were treated with oestradiol (40 mg/kg, intraperitoneally) and after 24 h the animals were sacrificed. The concentrations of NO and L-Arginine (L-Arg) were determined spectrophotometrically. For protein expressions of iNOS, p65 subunit of nuclear factor-κB (NFκB-p65), Ras homolog gene family-member A (RhoA), angiotensin II receptor type 1 (AT1R), insulin receptor substrate 1 (IRS-1), p85, p110 and protein kinase B (Akt), Western blot method was used. Coimmunoprecipitation was used for measuring the association of IRS-1 with the p85 subunit of phosphatidylinositol- 3-kinase (PI3K). The expression of iNOS messenger ribonucleic acid (mRNA) was measured with the quantitative real-time polymerase chain reaction (qRT-PCR). Immunohistochemical analysis of the tissue was used to detect localization and expression of iNOS in heart tissue. RESULTS: Oestradiol treatment reduced L-Arg concentration (p<0.01), iNOS mRNA (p<0.01) and protein (p<0.001) expression, level of RhoA (p<0.05) and AT1R (p<0.001) protein. In contrast, plasma NO (p<0.05), Akt phosphorylation at Thr308 (p<0.05) and protein level of p85 (p<0.001) increased after oestradiol treatment. CONCLUSION: Our results suggest that oestradiol in vivo regulates cardiac iNOS expression via the PI3K/Akt signaling pathway, through attenuation of RhoA and AT1R.
T2  - Current Vascular Pharmacology
T1  - Involvement of PI3K, Akt and RhoA in Oestradiol Regulation of Cardiac iNOS Expression
VL  - 17
IS  - 3
SP  - 307
EP  - 318
DO  - 10.2174/1570161116666180212142414
ER  - 

@article{
author = "Zafirović, Sonja and Sudar-Milovanović, Emina and Obradović, Milan M. and Đorđević, Jelena D. and Jasnić, Nebojša and Labudović-Borović, Milica and Isenović, Esma R.",
year = "2019",
abstract = "BACKGROUND: Oestradiol is an important regulatory factor with several positive effects on the cardiovascular (CV) system. We evaluated the molecular mechanism of the in vivo effects of oestradiol on the regulation of cardiac inducible nitric oxide (NO) synthase (iNOS) expression and activity. METHODS: Male Wistar rats were treated with oestradiol (40 mg/kg, intraperitoneally) and after 24 h the animals were sacrificed. The concentrations of NO and L-Arginine (L-Arg) were determined spectrophotometrically. For protein expressions of iNOS, p65 subunit of nuclear factor-κB (NFκB-p65), Ras homolog gene family-member A (RhoA), angiotensin II receptor type 1 (AT1R), insulin receptor substrate 1 (IRS-1), p85, p110 and protein kinase B (Akt), Western blot method was used. Coimmunoprecipitation was used for measuring the association of IRS-1 with the p85 subunit of phosphatidylinositol- 3-kinase (PI3K). The expression of iNOS messenger ribonucleic acid (mRNA) was measured with the quantitative real-time polymerase chain reaction (qRT-PCR). Immunohistochemical analysis of the tissue was used to detect localization and expression of iNOS in heart tissue. RESULTS: Oestradiol treatment reduced L-Arg concentration (p<0.01), iNOS mRNA (p<0.01) and protein (p<0.001) expression, level of RhoA (p<0.05) and AT1R (p<0.001) protein. In contrast, plasma NO (p<0.05), Akt phosphorylation at Thr308 (p<0.05) and protein level of p85 (p<0.001) increased after oestradiol treatment. CONCLUSION: Our results suggest that oestradiol in vivo regulates cardiac iNOS expression via the PI3K/Akt signaling pathway, through attenuation of RhoA and AT1R.",
journal = "Current Vascular Pharmacology",
title = "Involvement of PI3K, Akt and RhoA in Oestradiol Regulation of Cardiac iNOS Expression",
volume = "17",
number = "3",
pages = "307-318",
doi = "10.2174/1570161116666180212142414"
}

Zafirović, S., Sudar-Milovanović, E., Obradović, M. M., Đorđević, J. D., Jasnić, N., Labudović-Borović, M.,& Isenović, E. R.. (2019). Involvement of PI3K, Akt and RhoA in Oestradiol Regulation of Cardiac iNOS Expression. in Current Vascular Pharmacology, 17(3), 307-318.
https://doi.org/10.2174/1570161116666180212142414

Zafirović S, Sudar-Milovanović E, Obradović MM, Đorđević JD, Jasnić N, Labudović-Borović M, Isenović ER. Involvement of PI3K, Akt and RhoA in Oestradiol Regulation of Cardiac iNOS Expression. in Current Vascular Pharmacology. 2019;17(3):307-318.
doi:10.2174/1570161116666180212142414 .

Zafirović, Sonja, Sudar-Milovanović, Emina, Obradović, Milan M., Đorđević, Jelena D., Jasnić, Nebojša, Labudović-Borović, Milica, Isenović, Esma R., "Involvement of PI3K, Akt and RhoA in Oestradiol Regulation of Cardiac iNOS Expression" in Current Vascular Pharmacology, 17, no. 3 (2019):307-318,
https://doi.org/10.2174/1570161116666180212142414 . .

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Zafirović, Sonja
AU  - Soskić, Sanja S.
AU  - Stanimirović, Julijana
AU  - Trpković, Andreja
AU  - Jevremović, Danimir P.
AU  - Isenović, Esma R.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8483
AB  - Cardiovascular (CV) diseases are the most common health problems worldwide, with a permanent increase in incidence. Growing evidence underlines that insulin-like growth factor 1 (IGF-1) is a very important hormone responsible for normal CV system physiology. IGF-1 is an anabolic growth hormone, responsible for cell growth, differentiation, proliferation, and survival. Despite systemic effects, IGF-1 exerts a wide array of influences in the CV system affecting metabolic homeostasis, vasorelaxation, cardiac contractility and hypertrophy, autophagy, apoptosis, and antioxidative processes. The vasodilatory effect of IGF-1, is achieved through the regulation of the activity of endothelial nitric oxide synthase (eNOS) and, at least partly, through enhancing inducible NOS (iNOS) activity. Also, IGF-1 stimulates vascular relaxation through regulation of sodium/potassium-adenosine-triphosphatase. Numerous animal studies provided evidence of diverse influences of IGF-1 in the CV system such as vasorelaxation, anti-apoptotic and prosurvival effects. Human studies indicate that low serum levels of free or total IGF-1 contribute to an increased risk of CV and cerebrovascular disease. Large human trials aiming at finding clinical efficacy and outcome of IGF-1-related therapy are of great interest. We look forward to the development of new IGF 1 therapies with minor side effects. In this review, we discuss the latest literature data regarding the function of IGF-1 in the CV system in the physiological and pathophysiological conditions. © 2019 Bentham Science Publishers.
T2  - Current Pharmaceutical Design
T1  - Effects of IGF-1 on the cardiovascular system
VL  - 25
IS  - 35
SP  - 3715
EP  - 3725
DO  - 10.2174/1381612825666191106091507
ER  - 

@article{
author = "Obradović, Milan M. and Zafirović, Sonja and Soskić, Sanja S. and Stanimirović, Julijana and Trpković, Andreja and Jevremović, Danimir P. and Isenović, Esma R.",
year = "2019",
abstract = "Cardiovascular (CV) diseases are the most common health problems worldwide, with a permanent increase in incidence. Growing evidence underlines that insulin-like growth factor 1 (IGF-1) is a very important hormone responsible for normal CV system physiology. IGF-1 is an anabolic growth hormone, responsible for cell growth, differentiation, proliferation, and survival. Despite systemic effects, IGF-1 exerts a wide array of influences in the CV system affecting metabolic homeostasis, vasorelaxation, cardiac contractility and hypertrophy, autophagy, apoptosis, and antioxidative processes. The vasodilatory effect of IGF-1, is achieved through the regulation of the activity of endothelial nitric oxide synthase (eNOS) and, at least partly, through enhancing inducible NOS (iNOS) activity. Also, IGF-1 stimulates vascular relaxation through regulation of sodium/potassium-adenosine-triphosphatase. Numerous animal studies provided evidence of diverse influences of IGF-1 in the CV system such as vasorelaxation, anti-apoptotic and prosurvival effects. Human studies indicate that low serum levels of free or total IGF-1 contribute to an increased risk of CV and cerebrovascular disease. Large human trials aiming at finding clinical efficacy and outcome of IGF-1-related therapy are of great interest. We look forward to the development of new IGF 1 therapies with minor side effects. In this review, we discuss the latest literature data regarding the function of IGF-1 in the CV system in the physiological and pathophysiological conditions. © 2019 Bentham Science Publishers.",
journal = "Current Pharmaceutical Design",
title = "Effects of IGF-1 on the cardiovascular system",
volume = "25",
number = "35",
pages = "3715-3725",
doi = "10.2174/1381612825666191106091507"
}

Obradović, M. M., Zafirović, S., Soskić, S. S., Stanimirović, J., Trpković, A., Jevremović, D. P.,& Isenović, E. R.. (2019). Effects of IGF-1 on the cardiovascular system. in Current Pharmaceutical Design, 25(35), 3715-3725.
https://doi.org/10.2174/1381612825666191106091507

Obradović MM, Zafirović S, Soskić SS, Stanimirović J, Trpković A, Jevremović DP, Isenović ER. Effects of IGF-1 on the cardiovascular system. in Current Pharmaceutical Design. 2019;25(35):3715-3725.
doi:10.2174/1381612825666191106091507 .

Obradović, Milan M., Zafirović, Sonja, Soskić, Sanja S., Stanimirović, Julijana, Trpković, Andreja, Jevremović, Danimir P., Isenović, Esma R., "Effects of IGF-1 on the cardiovascular system" in Current Pharmaceutical Design, 25, no. 35 (2019):3715-3725,
https://doi.org/10.2174/1381612825666191106091507 . .

TY  - JOUR
AU  - Panić, Anastasija
AU  - Stanimirović, Julijana
AU  - Obradović, Milan M.
AU  - Zafirović, Sonja
AU  - Sudar-Milovanović, Emina
AU  - Petrović, Nina
AU  - Isenović, Esma R.
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8013
AB  - 17β-Estradiol (E2) is known to negatively regulate inducible nitric oxide (NO) synthase (iNOS) expression via estrogen receptor alpha (ER-α) activation in aortic vascular smooth muscle cells.Therefore, we sought to determine whether E2 can inhibit iNOS in vivo in hepatic tissue via the activation of ER-α and whether extracellular signal-regulated kinases 1/2 (ERK1/2)-miR-221 axis is involved in this process. Male Wistar rats were treated with a bolus injection of E2 intraperitoneally (40 μg/kg), and 24 hours after treatment the animals were sacrificed and the livers excised. The protein levels of iNOS, p50 and p65 subunits of nuclear factor κB (NFκB), ERα, ERK1/2 and protein kinase B (Akt), as well as the association of ERα/Src in liver lysates were assessed by Western blot. The expression of hepatic miR-221 was analyzed by qRT-PCR. Results show that E2 reduced hepatic iNOS protein expression (p less than 0.01), the protein level of ERα (p less than 0.05), ERK1/2 (p less than 0.05), Akt phosphorylation (p less than 0.001) and miR-221 expression (p less than 0.05). In contrast, hepatic ERα/Src kinase association level (p less than 0.05) increased after E2 treatment. Our results indicate that E2 inhibits hepatic iNOS via molecular mechanisms involving the activation of the ER-α and inhibition of ERK1/2-miR-221 axis.
T2  - Journal of biological regulators and homeostatic agents
T1  - 17β-estradiol inhibits hepatic iNOS via the activation of the estrogen receptor ER-α and inhibition of erk1/2-mir-221 axis
VL  - 32
IS  - 6
SP  - 1369
EP  - 1377
UR  - https://hdl.handle.net/21.15107/rcub_vinar_8013
ER  - 

@article{
author = "Panić, Anastasija and Stanimirović, Julijana and Obradović, Milan M. and Zafirović, Sonja and Sudar-Milovanović, Emina and Petrović, Nina and Isenović, Esma R.",
year = "2018",
abstract = "17β-Estradiol (E2) is known to negatively regulate inducible nitric oxide (NO) synthase (iNOS) expression via estrogen receptor alpha (ER-α) activation in aortic vascular smooth muscle cells.Therefore, we sought to determine whether E2 can inhibit iNOS in vivo in hepatic tissue via the activation of ER-α and whether extracellular signal-regulated kinases 1/2 (ERK1/2)-miR-221 axis is involved in this process. Male Wistar rats were treated with a bolus injection of E2 intraperitoneally (40 μg/kg), and 24 hours after treatment the animals were sacrificed and the livers excised. The protein levels of iNOS, p50 and p65 subunits of nuclear factor κB (NFκB), ERα, ERK1/2 and protein kinase B (Akt), as well as the association of ERα/Src in liver lysates were assessed by Western blot. The expression of hepatic miR-221 was analyzed by qRT-PCR. Results show that E2 reduced hepatic iNOS protein expression (p less than 0.01), the protein level of ERα (p less than 0.05), ERK1/2 (p less than 0.05), Akt phosphorylation (p less than 0.001) and miR-221 expression (p less than 0.05). In contrast, hepatic ERα/Src kinase association level (p less than 0.05) increased after E2 treatment. Our results indicate that E2 inhibits hepatic iNOS via molecular mechanisms involving the activation of the ER-α and inhibition of ERK1/2-miR-221 axis.",
journal = "Journal of biological regulators and homeostatic agents",
title = "17β-estradiol inhibits hepatic iNOS via the activation of the estrogen receptor ER-α and inhibition of erk1/2-mir-221 axis",
volume = "32",
number = "6",
pages = "1369-1377",
url = "https://hdl.handle.net/21.15107/rcub_vinar_8013"
}

Panić, A., Stanimirović, J., Obradović, M. M., Zafirović, S., Sudar-Milovanović, E., Petrović, N.,& Isenović, E. R.. (2018). 17β-estradiol inhibits hepatic iNOS via the activation of the estrogen receptor ER-α and inhibition of erk1/2-mir-221 axis. in Journal of biological regulators and homeostatic agents, 32(6), 1369-1377.
https://hdl.handle.net/21.15107/rcub_vinar_8013

Panić A, Stanimirović J, Obradović MM, Zafirović S, Sudar-Milovanović E, Petrović N, Isenović ER. 17β-estradiol inhibits hepatic iNOS via the activation of the estrogen receptor ER-α and inhibition of erk1/2-mir-221 axis. in Journal of biological regulators and homeostatic agents. 2018;32(6):1369-1377.
https://hdl.handle.net/21.15107/rcub_vinar_8013 .

Panić, Anastasija, Stanimirović, Julijana, Obradović, Milan M., Zafirović, Sonja, Sudar-Milovanović, Emina, Petrović, Nina, Isenović, Esma R., "17β-estradiol inhibits hepatic iNOS via the activation of the estrogen receptor ER-α and inhibition of erk1/2-mir-221 axis" in Journal of biological regulators and homeostatic agents, 32, no. 6 (2018):1369-1377,
https://hdl.handle.net/21.15107/rcub_vinar_8013 .

TY  - CONF
AU  - Stanimirović, Julijana
AU  - Panić, Anastasija
AU  - Obradović, Milan M.
AU  - Zafirović, Sonja
AU  - Isenović, Esma R.
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0021915018307147
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7948
C3  - Atherosclerosis
T1  - IGF-1 ameliorates detrimental effects of obesity in rat heart by promoting akt and FOXO1
VL  - 275
SP  - e137
DO  - 10.1016/j.atherosclerosis.2018.06.402
ER  - 

@conference{
author = "Stanimirović, Julijana and Panić, Anastasija and Obradović, Milan M. and Zafirović, Sonja and Isenović, Esma R.",
year = "2018",
journal = "Atherosclerosis",
title = "IGF-1 ameliorates detrimental effects of obesity in rat heart by promoting akt and FOXO1",
volume = "275",
pages = "e137",
doi = "10.1016/j.atherosclerosis.2018.06.402"
}

Stanimirović, J., Panić, A., Obradović, M. M., Zafirović, S.,& Isenović, E. R.. (2018). IGF-1 ameliorates detrimental effects of obesity in rat heart by promoting akt and FOXO1. in Atherosclerosis, 275, e137.
https://doi.org/10.1016/j.atherosclerosis.2018.06.402

Stanimirović J, Panić A, Obradović MM, Zafirović S, Isenović ER. IGF-1 ameliorates detrimental effects of obesity in rat heart by promoting akt and FOXO1. in Atherosclerosis. 2018;275:e137.
doi:10.1016/j.atherosclerosis.2018.06.402 .

Stanimirović, Julijana, Panić, Anastasija, Obradović, Milan M., Zafirović, Sonja, Isenović, Esma R., "IGF-1 ameliorates detrimental effects of obesity in rat heart by promoting akt and FOXO1" in Atherosclerosis, 275 (2018):e137,
https://doi.org/10.1016/j.atherosclerosis.2018.06.402 . .