TY  - JOUR
AU  - Stepanović, Aleksandar
AU  - Nikitović, Marina
AU  - Stanojković, Tatjana P.
AU  - Grujičić, Danica
AU  - Bukumirić, Zoran
AU  - Srbljak, Ivana
AU  - Ilić, Rosanda
AU  - Milošević, Snežana
AU  - Arsenijević, Tatjana
AU  - Petrović, Nina
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10265
AB  - A personalized approach to chemoradiation is important in reducing its potential side effects and identifying a group of patients prone to toxicity. MicroRNAs have been shown to have a predictive potential for radiotoxicity. The goal of the study was to test if levels of miRNA in peripheral blood mononuclear cells of glioblastoma patients are associated with toxicity and to identify the peak time point for toxicity. MicroRNA-10b/21/34a levels were measured in 43 patients with and without toxicity, at baseline, at the 15th, and at the 30th fraction by Real-Time quantitative Polymerase Chain Reaction. MicroRNA-10b/21 levels increased with toxicity grade (p = 0.014; p = 0.013); miR-21/34a levels were significantly different between patients with and without toxicity at the 15th fraction (p = 0.030; p = 0.045), while miR-34a levels significantly changed during treatment (p < 0.001). All three miRNAs showed a significantly high positive correlation with one another. MiR-34a might be considered as a predictive factor for toxicity due to its changes during treatment, and differences between the groups with and without toxicity; miR-10b might be used to predict toxicity; miR-10b/21 might be used for predicting the grade of toxicity in GB patients.
T2  - Scientific Reports
T1  - Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide
VL  - 12
IS  - 1
SP  - 7505
DO  - 10.1038/s41598-022-11445-9
ER  - 

@article{
author = "Stepanović, Aleksandar and Nikitović, Marina and Stanojković, Tatjana P. and Grujičić, Danica and Bukumirić, Zoran and Srbljak, Ivana and Ilić, Rosanda and Milošević, Snežana and Arsenijević, Tatjana and Petrović, Nina",
year = "2022",
abstract = "A personalized approach to chemoradiation is important in reducing its potential side effects and identifying a group of patients prone to toxicity. MicroRNAs have been shown to have a predictive potential for radiotoxicity. The goal of the study was to test if levels of miRNA in peripheral blood mononuclear cells of glioblastoma patients are associated with toxicity and to identify the peak time point for toxicity. MicroRNA-10b/21/34a levels were measured in 43 patients with and without toxicity, at baseline, at the 15th, and at the 30th fraction by Real-Time quantitative Polymerase Chain Reaction. MicroRNA-10b/21 levels increased with toxicity grade (p = 0.014; p = 0.013); miR-21/34a levels were significantly different between patients with and without toxicity at the 15th fraction (p = 0.030; p = 0.045), while miR-34a levels significantly changed during treatment (p < 0.001). All three miRNAs showed a significantly high positive correlation with one another. MiR-34a might be considered as a predictive factor for toxicity due to its changes during treatment, and differences between the groups with and without toxicity; miR-10b might be used to predict toxicity; miR-10b/21 might be used for predicting the grade of toxicity in GB patients.",
journal = "Scientific Reports",
title = "Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide",
volume = "12",
number = "1",
pages = "7505",
doi = "10.1038/s41598-022-11445-9"
}

Stepanović, A., Nikitović, M., Stanojković, T. P., Grujičić, D., Bukumirić, Z., Srbljak, I., Ilić, R., Milošević, S., Arsenijević, T.,& Petrović, N.. (2022). Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide. in Scientific Reports, 12(1), 7505.
https://doi.org/10.1038/s41598-022-11445-9

Stepanović A, Nikitović M, Stanojković TP, Grujičić D, Bukumirić Z, Srbljak I, Ilić R, Milošević S, Arsenijević T, Petrović N. Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide. in Scientific Reports. 2022;12(1):7505.
doi:10.1038/s41598-022-11445-9 .

Stepanović, Aleksandar, Nikitović, Marina, Stanojković, Tatjana P., Grujičić, Danica, Bukumirić, Zoran, Srbljak, Ivana, Ilić, Rosanda, Milošević, Snežana, Arsenijević, Tatjana, Petrović, Nina, "Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide" in Scientific Reports, 12, no. 1 (2022):7505,
https://doi.org/10.1038/s41598-022-11445-9 . .

TY  - JOUR
AU  - Stanojković, Tatjana P.
AU  - Matić, Ivana Z.
AU  - Petrović, Nina
AU  - Stanković, Vesna
AU  - Kopčalić, Katarina
AU  - Besu, Irina
AU  - Đorđić Crnogorac, Marija
AU  - Mališić, Emina
AU  - Mirjačić-Martinović, Katarina
AU  - Vuletić, Ana
AU  - Bukumirić, Zoran
AU  - Žižak, Željko
AU  - Veldwijk, Marlon
AU  - Herskind, Carsten
AU  - Nikitović, Marina
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9713
AB  - One of the challenges of radiation oncology in the era of personalized medicine is identification of biomarkers associated with individual radiosensitivity. The aim of research was to evaluate the possible clinical value of the associations between clinical, physical, and biological factors, and risk for development of acute radiotoxicity in patients with prostate cancer. The study involved forty four patients treated with three-dimensional conformal radiotherapy. The concentrations of IL-1β, IL-2, IL-6, IFN-γ and TGF-β1 were assessed before radiotherapy, after 5th, 15th and 25th radiotherapy fractions, at the end, and 1 month after the end of radiotherapy. Cytokine gene expression was determined in peripheral blood mononuclear cells. The univariate analysis of circulating cytokine levels during radiotherapy showed that increased serum concentrations of IL-6 were significantly associated with higher grade of acute genitourinary toxicity. The multivariate analysis demonstrated that increased level of IL-6 during the radiotherapy was significantly associated with higher grade of acute genitourinary toxicity across treatment. TGF-β expression levels significantly decreased during course of radiotherapy. Research indicates that changes in circulating cytokine levels might be important parameter of radiotoxicity in patients with prostate cancer. These findings suggest that future studies based on multi-parameter examination are necessary for prediction of individual radiosensitivity.
T2  - Scientific Reports
T1  - Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients
VL  - 10
IS  - 1
SP  - 19002
DO  - 10.1038/s41598-020-75812-0
ER  - 

@article{
author = "Stanojković, Tatjana P. and Matić, Ivana Z. and Petrović, Nina and Stanković, Vesna and Kopčalić, Katarina and Besu, Irina and Đorđić Crnogorac, Marija and Mališić, Emina and Mirjačić-Martinović, Katarina and Vuletić, Ana and Bukumirić, Zoran and Žižak, Željko and Veldwijk, Marlon and Herskind, Carsten and Nikitović, Marina",
year = "2020",
abstract = "One of the challenges of radiation oncology in the era of personalized medicine is identification of biomarkers associated with individual radiosensitivity. The aim of research was to evaluate the possible clinical value of the associations between clinical, physical, and biological factors, and risk for development of acute radiotoxicity in patients with prostate cancer. The study involved forty four patients treated with three-dimensional conformal radiotherapy. The concentrations of IL-1β, IL-2, IL-6, IFN-γ and TGF-β1 were assessed before radiotherapy, after 5th, 15th and 25th radiotherapy fractions, at the end, and 1 month after the end of radiotherapy. Cytokine gene expression was determined in peripheral blood mononuclear cells. The univariate analysis of circulating cytokine levels during radiotherapy showed that increased serum concentrations of IL-6 were significantly associated with higher grade of acute genitourinary toxicity. The multivariate analysis demonstrated that increased level of IL-6 during the radiotherapy was significantly associated with higher grade of acute genitourinary toxicity across treatment. TGF-β expression levels significantly decreased during course of radiotherapy. Research indicates that changes in circulating cytokine levels might be important parameter of radiotoxicity in patients with prostate cancer. These findings suggest that future studies based on multi-parameter examination are necessary for prediction of individual radiosensitivity.",
journal = "Scientific Reports",
title = "Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients",
volume = "10",
number = "1",
pages = "19002",
doi = "10.1038/s41598-020-75812-0"
}

Stanojković, T. P., Matić, I. Z., Petrović, N., Stanković, V., Kopčalić, K., Besu, I., Đorđić Crnogorac, M., Mališić, E., Mirjačić-Martinović, K., Vuletić, A., Bukumirić, Z., Žižak, Ž., Veldwijk, M., Herskind, C.,& Nikitović, M.. (2020). Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients. in Scientific Reports, 10(1), 19002.
https://doi.org/10.1038/s41598-020-75812-0

Stanojković TP, Matić IZ, Petrović N, Stanković V, Kopčalić K, Besu I, Đorđić Crnogorac M, Mališić E, Mirjačić-Martinović K, Vuletić A, Bukumirić Z, Žižak Ž, Veldwijk M, Herskind C, Nikitović M. Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients. in Scientific Reports. 2020;10(1):19002.
doi:10.1038/s41598-020-75812-0 .

Stanojković, Tatjana P., Matić, Ivana Z., Petrović, Nina, Stanković, Vesna, Kopčalić, Katarina, Besu, Irina, Đorđić Crnogorac, Marija, Mališić, Emina, Mirjačić-Martinović, Katarina, Vuletić, Ana, Bukumirić, Zoran, Žižak, Željko, Veldwijk, Marlon, Herskind, Carsten, Nikitović, Marina, "Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients" in Scientific Reports, 10, no. 1 (2020):19002,
https://doi.org/10.1038/s41598-020-75812-0 . .

TY  - JOUR
AU  - Kopčalić, Katarina
AU  - Petrović, Nina
AU  - Stanojković, Tatjana P.
AU  - Stanković, Vesna
AU  - Bukumirić, Zoran
AU  - Roganović, Jelena
AU  - Mališić, Emina
AU  - Nikitović, Marina
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8444
AB  - Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative ΔΔCt method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs. © 2018 Elsevier GmbH
T2  - Pathology - Research and Practice
T1  - Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study
VL  - 215
IS  - 4
SP  - 626
EP  - 631
DO  - 10.1016/j.prp.2018.12.007
ER  - 

@article{
author = "Kopčalić, Katarina and Petrović, Nina and Stanojković, Tatjana P. and Stanković, Vesna and Bukumirić, Zoran and Roganović, Jelena and Mališić, Emina and Nikitović, Marina",
year = "2019",
abstract = "Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative ΔΔCt method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs. © 2018 Elsevier GmbH",
journal = "Pathology - Research and Practice",
title = "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study",
volume = "215",
number = "4",
pages = "626-631",
doi = "10.1016/j.prp.2018.12.007"
}

Kopčalić, K., Petrović, N., Stanojković, T. P., Stanković, V., Bukumirić, Z., Roganović, J., Mališić, E.,& Nikitović, M.. (2019). Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology - Research and Practice, 215(4), 626-631.
https://doi.org/10.1016/j.prp.2018.12.007

Kopčalić K, Petrović N, Stanojković TP, Stanković V, Bukumirić Z, Roganović J, Mališić E, Nikitović M. Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology - Research and Practice. 2019;215(4):626-631.
doi:10.1016/j.prp.2018.12.007 .

Kopčalić, Katarina, Petrović, Nina, Stanojković, Tatjana P., Stanković, Vesna, Bukumirić, Zoran, Roganović, Jelena, Mališić, Emina, Nikitović, Marina, "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study" in Pathology - Research and Practice, 215, no. 4 (2019):626-631,
https://doi.org/10.1016/j.prp.2018.12.007 . .