TY  - CONF
AU  - Životić, Ivan
AU  - Jovanović, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Dekleva, Milica
AU  - Marković Nikolić, Nevena
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12816
AB  - Introduction: Recently, rs2275888 eQTL in PTPLAD2 gene has been associated with expression of several loci, during inflammatory stimulation in monocytes. Myocardial infarction (MI) triggers an intense inflammatory response that is essential for cardiac repair. We aimed to perform data scouting in appropriate rs2275888 genotype model to identify differentially expressed genes (DEGs), their biological meaning, and key miRs potentially associated with rs2275888 eQTL in peripheral blood mononuclear leucocytes (PBML) of MI patients 6 months after first MI. Methods: Transcriptome data was obtained from PBMLs of 21 patients, who suffered ischemic MI, by employing Illumina iScan microarray technology. Genotyping for rs2275888 was conducted with real-time PCR, using TaqMan® assay. Preprocessing and identification of DEGs was done using limma package of R/Bioconductor software. The online tool DAVID v6.8 was employed for functional enrichment analysis. Most important miRs were selected using NetworkAnalyst web tool, based on the degree centrality value. Results: Transcriptome analysis in recessive model TT+TC (n=19) vs. CC (n=2) identified 68 DEGs. Top significant biological processes involving DEGs cover vascular physiology, cell growth and signaling. Pathway analysis associated DEGs with adherens junction, Rap1 and Ras signaling. Network analysis identified hsa-miR335-5p, -26b-5p, -93-5p, -16-5p, -124-3p, -20b-5p, -17-5p and -218-5p as miRs with top centrality degree in our DEGs list. Conclusion: Seven of eight detected miRs have already been described in MI pathology or suggested as potential biomarkers for MI. Our result suggest the importance of integration of eQTLs, biological processes and pathway analysis coupled with miR activity for further research in MI pathology.
PB  - Belgrade : University of Belgrade, Faculty of Biology
C3  - CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
T1  - Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach
SP  - 176
EP  - 176
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12816
ER  - 

@conference{
author = "Životić, Ivan and Jovanović, Ivan and Đurić, Tamara and Stanković, Aleksandra and Dekleva, Milica and Marković Nikolić, Nevena and Alavantić, Dragan and Živković, Maja",
year = "2017",
abstract = "Introduction: Recently, rs2275888 eQTL in PTPLAD2 gene has been associated with expression of several loci, during inflammatory stimulation in monocytes. Myocardial infarction (MI) triggers an intense inflammatory response that is essential for cardiac repair. We aimed to perform data scouting in appropriate rs2275888 genotype model to identify differentially expressed genes (DEGs), their biological meaning, and key miRs potentially associated with rs2275888 eQTL in peripheral blood mononuclear leucocytes (PBML) of MI patients 6 months after first MI. Methods: Transcriptome data was obtained from PBMLs of 21 patients, who suffered ischemic MI, by employing Illumina iScan microarray technology. Genotyping for rs2275888 was conducted with real-time PCR, using TaqMan® assay. Preprocessing and identification of DEGs was done using limma package of R/Bioconductor software. The online tool DAVID v6.8 was employed for functional enrichment analysis. Most important miRs were selected using NetworkAnalyst web tool, based on the degree centrality value. Results: Transcriptome analysis in recessive model TT+TC (n=19) vs. CC (n=2) identified 68 DEGs. Top significant biological processes involving DEGs cover vascular physiology, cell growth and signaling. Pathway analysis associated DEGs with adherens junction, Rap1 and Ras signaling. Network analysis identified hsa-miR335-5p, -26b-5p, -93-5p, -16-5p, -124-3p, -20b-5p, -17-5p and -218-5p as miRs with top centrality degree in our DEGs list. Conclusion: Seven of eight detected miRs have already been described in MI pathology or suggested as potential biomarkers for MI. Our result suggest the importance of integration of eQTLs, biological processes and pathway analysis coupled with miR activity for further research in MI pathology.",
publisher = "Belgrade : University of Belgrade, Faculty of Biology",
journal = "CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts",
title = "Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach",
pages = "176-176",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12816"
}

Životić, I., Jovanović, I., Đurić, T., Stanković, A., Dekleva, M., Marković Nikolić, N., Alavantić, D.,& Živković, M.. (2017). Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
Belgrade : University of Belgrade, Faculty of Biology., 176-176.
https://hdl.handle.net/21.15107/rcub_vinar_12816

Životić I, Jovanović I, Đurić T, Stanković A, Dekleva M, Marković Nikolić N, Alavantić D, Živković M. Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts. 2017;:176-176.
https://hdl.handle.net/21.15107/rcub_vinar_12816 .

Životić, Ivan, Jovanović, Ivan, Đurić, Tamara, Stanković, Aleksandra, Dekleva, Milica, Marković Nikolić, Nevena, Alavantić, Dragan, Živković, Maja, "Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach" in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts (2017):176-176,
https://hdl.handle.net/21.15107/rcub_vinar_12816 .

TY  - CONF
AU  - Životić, Ivan
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Đorđević, Ana
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nevena
AU  - Alavantić, Dragan
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7178
AB  - Aim: Myocardial infarction (MI) is the clinical complication predominately
caused by coronary plaque buildup and rupture during the process of
atherosclerosis. In the 9p21 locus two important risk haplotype blocks
have been identified. The one that carries the risk for MI with the lead
variant rs10757278 and another characterized to influence progression of
the MI, with the lead variant rs518394. We have investigated association of
the two genetic variants with the ST-elevated MI in the gender specific
manner. We have also tested variants effect on p15 mRNA level as one of
the possible mechanisms of the variants effect.Methods: The study group included 147 patients (72 females) with angiographically assessed MI, and 240 healthy controls (90 females). DNA
and RNA (n¼28) where isolated from peripheral blood mono nuclear
leukocytes. Genotypes for rs10757278 A/G and rs518394 C/G, and relative
mRNA level for p15 were determined using commercial TaqMan® assays
on 7500 ABI Real-Time PCR.
Results: We have found significant association of rs10757278 GG with STelevated MI occurrence, with OR of 2.2 (CI¼1.07-4.5, p¼0.03) in females.
P15 mRNA was significantly down-regulated in G allele carriers (AG+GG vs
AA) by a mean factor of 0.449 (S.E. range is 0.188-1.059), p¼0.019 in the
whole group. The genetic variant rs518394 was not significantly associated
either with MI or p15 mRNA level.
Conclusions: Genotype GG of rs10757278 is significantly associated with
MI occurrence in females in Serbian population. On
C3  - Atherosclerosis
T1  - RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia
VL  - 263
SP  - e188
EP  - e188
DO  - 10.1016/j.atherosclerosis.2017.06.603
ER  - 

@conference{
author = "Životić, Ivan and Živković, Maja and Đurić, Tamara and Stanković, Aleksandra and Đorđević, Ana and Dekleva, Milica and Marković-Nikolić, Nevena and Alavantić, Dragan",
year = "2017",
abstract = "Aim: Myocardial infarction (MI) is the clinical complication predominately
caused by coronary plaque buildup and rupture during the process of
atherosclerosis. In the 9p21 locus two important risk haplotype blocks
have been identified. The one that carries the risk for MI with the lead
variant rs10757278 and another characterized to influence progression of
the MI, with the lead variant rs518394. We have investigated association of
the two genetic variants with the ST-elevated MI in the gender specific
manner. We have also tested variants effect on p15 mRNA level as one of
the possible mechanisms of the variants effect.Methods: The study group included 147 patients (72 females) with angiographically assessed MI, and 240 healthy controls (90 females). DNA
and RNA (n¼28) where isolated from peripheral blood mono nuclear
leukocytes. Genotypes for rs10757278 A/G and rs518394 C/G, and relative
mRNA level for p15 were determined using commercial TaqMan® assays
on 7500 ABI Real-Time PCR.
Results: We have found significant association of rs10757278 GG with STelevated MI occurrence, with OR of 2.2 (CI¼1.07-4.5, p¼0.03) in females.
P15 mRNA was significantly down-regulated in G allele carriers (AG+GG vs
AA) by a mean factor of 0.449 (S.E. range is 0.188-1.059), p¼0.019 in the
whole group. The genetic variant rs518394 was not significantly associated
either with MI or p15 mRNA level.
Conclusions: Genotype GG of rs10757278 is significantly associated with
MI occurrence in females in Serbian population. On",
journal = "Atherosclerosis",
title = "RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia",
volume = "263",
pages = "e188-e188",
doi = "10.1016/j.atherosclerosis.2017.06.603"
}

Životić, I., Živković, M., Đurić, T., Stanković, A., Đorđević, A., Dekleva, M., Marković-Nikolić, N.,& Alavantić, D.. (2017). RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia. in Atherosclerosis, 263, e188-e188.
https://doi.org/10.1016/j.atherosclerosis.2017.06.603

Životić I, Živković M, Đurić T, Stanković A, Đorđević A, Dekleva M, Marković-Nikolić N, Alavantić D. RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia. in Atherosclerosis. 2017;263:e188-e188.
doi:10.1016/j.atherosclerosis.2017.06.603 .

Životić, Ivan, Živković, Maja, Đurić, Tamara, Stanković, Aleksandra, Đorđević, Ana, Dekleva, Milica, Marković-Nikolić, Nevena, Alavantić, Dragan, "RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia" in Atherosclerosis, 263 (2017):e188-e188,
https://doi.org/10.1016/j.atherosclerosis.2017.06.603 . .