TY  - JOUR
AU  - Radaković-Ćosić, Jovana
AU  - Miković, Željko
AU  - Mandić-Rajčević, Stefan
AU  - Sudar-Milovanović, Emina
AU  - Stojsavljević, Aleksandar
AU  - Nikolić, Gorana
AU  - Radojičić, Ognjen
AU  - Perović, Milan
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10846
AB  - Infertility is a major global health issue, affecting approximately 9% of couples worldwide. Its prevalence increased steadily over the preceding decades, paralleling environmental and lifestyle changes. One assisted reproductive technique to overcome infertility is in vitro fertilization (IVF). The IVF procedure consists of several stages, one of which is controlled ovarian stimulation (COS) via various protocols allowing follicular recruitment and maturation in preparation for oocyte retrieval. Follicular fluid (FF) is the microenvironment in which oocytes develop during folliculogenesis, and FF information could be used to assess follicle and oocyte development and maturation stages. Nitric oxide (NO) is a component of FF that is being studied as a predictive factor of follicle maturation and egg quality and as a potential indicator of the success of COS during the IVF process. The hypothesis was that COS affects NO levels in the serum; more specifically, that NO levels in serum after COS correlate with NO levels in FF, based on literature data on the influence of female reproductive hormones on NO metabolism and data on the change in the hormonal milieu affected by COS use. Furthermore, it was hypothesized that NO levels in serum and FF after COS were related to the number of high-quality female reproductive cells obtained during IVF. Assuming that NO levels in serum after COS correlate with NO levels in FF, the central hypothesis of this study is that serum NO levels after COS could be a valuable predictor of oocyte quality and the number of high-quality female reproductive cells achieved by COS. As a result of the hypothesis, measuring NO could be a novel way to improve the efficiency of IVF treatment.
T2  - Medical Hypotheses
T1  - Does controlled ovarian stimulation during in vitro fertilization affect the level of nitric oxide a potential indicator of oocyte quality?
VL  - 174
SP  - 111061
DO  - 10.1016/j.mehy.2023.111061
ER  - 

@article{
author = "Radaković-Ćosić, Jovana and Miković, Željko and Mandić-Rajčević, Stefan and Sudar-Milovanović, Emina and Stojsavljević, Aleksandar and Nikolić, Gorana and Radojičić, Ognjen and Perović, Milan",
year = "2023",
abstract = "Infertility is a major global health issue, affecting approximately 9% of couples worldwide. Its prevalence increased steadily over the preceding decades, paralleling environmental and lifestyle changes. One assisted reproductive technique to overcome infertility is in vitro fertilization (IVF). The IVF procedure consists of several stages, one of which is controlled ovarian stimulation (COS) via various protocols allowing follicular recruitment and maturation in preparation for oocyte retrieval. Follicular fluid (FF) is the microenvironment in which oocytes develop during folliculogenesis, and FF information could be used to assess follicle and oocyte development and maturation stages. Nitric oxide (NO) is a component of FF that is being studied as a predictive factor of follicle maturation and egg quality and as a potential indicator of the success of COS during the IVF process. The hypothesis was that COS affects NO levels in the serum; more specifically, that NO levels in serum after COS correlate with NO levels in FF, based on literature data on the influence of female reproductive hormones on NO metabolism and data on the change in the hormonal milieu affected by COS use. Furthermore, it was hypothesized that NO levels in serum and FF after COS were related to the number of high-quality female reproductive cells obtained during IVF. Assuming that NO levels in serum after COS correlate with NO levels in FF, the central hypothesis of this study is that serum NO levels after COS could be a valuable predictor of oocyte quality and the number of high-quality female reproductive cells achieved by COS. As a result of the hypothesis, measuring NO could be a novel way to improve the efficiency of IVF treatment.",
journal = "Medical Hypotheses",
title = "Does controlled ovarian stimulation during in vitro fertilization affect the level of nitric oxide a potential indicator of oocyte quality?",
volume = "174",
pages = "111061",
doi = "10.1016/j.mehy.2023.111061"
}

Radaković-Ćosić, J., Miković, Ž., Mandić-Rajčević, S., Sudar-Milovanović, E., Stojsavljević, A., Nikolić, G., Radojičić, O.,& Perović, M.. (2023). Does controlled ovarian stimulation during in vitro fertilization affect the level of nitric oxide a potential indicator of oocyte quality?. in Medical Hypotheses, 174, 111061.
https://doi.org/10.1016/j.mehy.2023.111061

Radaković-Ćosić J, Miković Ž, Mandić-Rajčević S, Sudar-Milovanović E, Stojsavljević A, Nikolić G, Radojičić O, Perović M. Does controlled ovarian stimulation during in vitro fertilization affect the level of nitric oxide a potential indicator of oocyte quality?. in Medical Hypotheses. 2023;174:111061.
doi:10.1016/j.mehy.2023.111061 .

Radaković-Ćosić, Jovana, Miković, Željko, Mandić-Rajčević, Stefan, Sudar-Milovanović, Emina, Stojsavljević, Aleksandar, Nikolić, Gorana, Radojičić, Ognjen, Perović, Milan, "Does controlled ovarian stimulation during in vitro fertilization affect the level of nitric oxide a potential indicator of oocyte quality?" in Medical Hypotheses, 174 (2023):111061,
https://doi.org/10.1016/j.mehy.2023.111061 . .

TY  - JOUR
AU  - Jeremić, Ana
AU  - Miković, Željko
AU  - Sudar-Milovanović, Emina
AU  - Isenović, Esma R.
AU  - Perović, Milan
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9971
AB  - Follicular and serum vitamin D are considered potential markers of the oocyte and embryos' quality and predictors of IVF outcomes.   Material and methods:  This retrospective cross-sectional study correlated vitamin D in sera and follicular fluid of women with unexplained...
T2  - Archives of Medical Science
T1  - Follicular and serum levels of vitamin D in women with unexplained infertility and their relationship with in vitro fertilization outcome: an observational pilot study
VL  - 17
IS  - 5
SP  - 1418
EP  - 1422
DO  - 10.5114/aoms/141185
ER  - 

@article{
author = "Jeremić, Ana and Miković, Željko and Sudar-Milovanović, Emina and Isenović, Esma R. and Perović, Milan",
year = "2021",
abstract = "Follicular and serum vitamin D are considered potential markers of the oocyte and embryos' quality and predictors of IVF outcomes.   Material and methods:  This retrospective cross-sectional study correlated vitamin D in sera and follicular fluid of women with unexplained...",
journal = "Archives of Medical Science",
title = "Follicular and serum levels of vitamin D in women with unexplained infertility and their relationship with in vitro fertilization outcome: an observational pilot study",
volume = "17",
number = "5",
pages = "1418-1422",
doi = "10.5114/aoms/141185"
}

Jeremić, A., Miković, Ž., Sudar-Milovanović, E., Isenović, E. R.,& Perović, M.. (2021). Follicular and serum levels of vitamin D in women with unexplained infertility and their relationship with in vitro fertilization outcome: an observational pilot study. in Archives of Medical Science, 17(5), 1418-1422.
https://doi.org/10.5114/aoms/141185

Jeremić A, Miković Ž, Sudar-Milovanović E, Isenović ER, Perović M. Follicular and serum levels of vitamin D in women with unexplained infertility and their relationship with in vitro fertilization outcome: an observational pilot study. in Archives of Medical Science. 2021;17(5):1418-1422.
doi:10.5114/aoms/141185 .

Jeremić, Ana, Miković, Željko, Sudar-Milovanović, Emina, Isenović, Esma R., Perović, Milan, "Follicular and serum levels of vitamin D in women with unexplained infertility and their relationship with in vitro fertilization outcome: an observational pilot study" in Archives of Medical Science, 17, no. 5 (2021):1418-1422,
https://doi.org/10.5114/aoms/141185 . .

TY  - JOUR
AU  - Perović, Milan
AU  - Sudar-Milovanović, Emina
AU  - Simonović, Ema D.
AU  - Resanović, Ivana
AU  - Draganić, Veselin D.
AU  - Radaković, Jovana D.
AU  - Soldatović, Ivan A.
AU  - Isenović, Esma R.
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0306987718310892
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8006
AB  - Controlled ovarian stimulation (COS) is used to augment the number of retrieved oocytes in in vitro fertilization (IVF). Follicular fluid (FF) contributes significantly to oocyte quality. Since the FF is composed of follicular secretions and plasma exudation, it reflects alterations in granulosa and thecal cells secretion as well as changes in the level of plasma constituents. Phospholipids (PL) and free fatty acids (FFA) are important constituents of both, FF and serum. Our hypothesis is that COS affects the level of PL and FFA in serum. Furthermore, since the level of PL and FFA in FF partially depends on their levels in serum, as a collaterally of our hypothesis is that the existing level of PL and FFA in serum correlates with the levels of PL and FFA in FF, and that the dose of applied gonadotropins during COS will correlate with the levels of PL and FFA in serum and FF. In addition, we assume that the level of PL and FFA in serum and in FF after COS will correlate with the retrieved number of GQ oocytes, one of the most important outcomes of COS.. © 2018
T2  - Medical Hypotheses
T1  - Hypothesis regarding the effects of gonadotropins on the level of free fatty acids and phospholipids in serum and follicular fluid during controlled ovarian stimulation
VL  - 123
SP  - 30
EP  - 34
DO  - 10.1016/j.mehy.2018.11.021
ER  - 

@article{
author = "Perović, Milan and Sudar-Milovanović, Emina and Simonović, Ema D. and Resanović, Ivana and Draganić, Veselin D. and Radaković, Jovana D. and Soldatović, Ivan A. and Isenović, Esma R.",
year = "2019",
abstract = "Controlled ovarian stimulation (COS) is used to augment the number of retrieved oocytes in in vitro fertilization (IVF). Follicular fluid (FF) contributes significantly to oocyte quality. Since the FF is composed of follicular secretions and plasma exudation, it reflects alterations in granulosa and thecal cells secretion as well as changes in the level of plasma constituents. Phospholipids (PL) and free fatty acids (FFA) are important constituents of both, FF and serum. Our hypothesis is that COS affects the level of PL and FFA in serum. Furthermore, since the level of PL and FFA in FF partially depends on their levels in serum, as a collaterally of our hypothesis is that the existing level of PL and FFA in serum correlates with the levels of PL and FFA in FF, and that the dose of applied gonadotropins during COS will correlate with the levels of PL and FFA in serum and FF. In addition, we assume that the level of PL and FFA in serum and in FF after COS will correlate with the retrieved number of GQ oocytes, one of the most important outcomes of COS.. © 2018",
journal = "Medical Hypotheses",
title = "Hypothesis regarding the effects of gonadotropins on the level of free fatty acids and phospholipids in serum and follicular fluid during controlled ovarian stimulation",
volume = "123",
pages = "30-34",
doi = "10.1016/j.mehy.2018.11.021"
}

Perović, M., Sudar-Milovanović, E., Simonović, E. D., Resanović, I., Draganić, V. D., Radaković, J. D., Soldatović, I. A.,& Isenović, E. R.. (2019). Hypothesis regarding the effects of gonadotropins on the level of free fatty acids and phospholipids in serum and follicular fluid during controlled ovarian stimulation. in Medical Hypotheses, 123, 30-34.
https://doi.org/10.1016/j.mehy.2018.11.021

Perović M, Sudar-Milovanović E, Simonović ED, Resanović I, Draganić VD, Radaković JD, Soldatović IA, Isenović ER. Hypothesis regarding the effects of gonadotropins on the level of free fatty acids and phospholipids in serum and follicular fluid during controlled ovarian stimulation. in Medical Hypotheses. 2019;123:30-34.
doi:10.1016/j.mehy.2018.11.021 .

Perović, Milan, Sudar-Milovanović, Emina, Simonović, Ema D., Resanović, Ivana, Draganić, Veselin D., Radaković, Jovana D., Soldatović, Ivan A., Isenović, Esma R., "Hypothesis regarding the effects of gonadotropins on the level of free fatty acids and phospholipids in serum and follicular fluid during controlled ovarian stimulation" in Medical Hypotheses, 123 (2019):30-34,
https://doi.org/10.1016/j.mehy.2018.11.021 . .

TY  - JOUR
AU  - Panić, Anastasija
AU  - Stanimirović, Julijana
AU  - Obradović, Milan M.
AU  - Sudar-Milovanović, Emina
AU  - Perović, Milan
AU  - Lačković, Milena
AU  - Petrović, Nina
AU  - Isenović, Esma R.
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8399
AB  - Purpose: This study aimed to investigate in vivo effects of estradiol on the regulation of hepatic inducible nitric oxide synthase (iNOS) expression in the high fat (HF) diet-induced obesity. Also, we aimed to investigate whether activation of the extracellular signal-regulated kinase (ERK1/2), adenosine monophosphate-activated protein kinase (AMPK), Src kinase, and miR-221 is involved in estradiol-mediated regulation of iNOS in the liver of obese male Wistar rats. Male Wistar rats were fed a standard laboratory diet or a HF diet for 10 weeks. Half of HF rats were treated with estradiol intraperitoneally (40 μg/kg), whereas the other half were placebo-treated 24 H before euthanasia. Results show that estradiol treatment of HF rats decreased hepatic iNOS mRNA (P < 0.05) and protein expression (P < 0.01), the protein levels of p65 subunit of nuclear factor κB (P < 0.05) and ERα (P < 0.05), ERK1/2 phosphorylation (P < 0.001), and ERα/Src kinase association (P < 0.05). By contrast, hepatic Src protein level (P < 0.05), AMPKα phosphorylation (P < 0.05), and miR-221 expression (P < 0.05) were increased in HF rats after estradiol treatment. Our results indicate that estradiol in vivo regulates hepatic iNOS expression in obese rats via molecular mechanisms involving ERK1/2, AMPK, Src, and miR-221 signaling. © 2018 International Union of Biochemistry and Molecular Biology, Inc.
T2  - Biotechnology and Applied Biochemistry
T1  - Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221
VL  - 65
IS  - 6
SP  - 797
EP  - 806
DO  - 10.1002/bab.1680
ER  - 

@article{
author = "Panić, Anastasija and Stanimirović, Julijana and Obradović, Milan M. and Sudar-Milovanović, Emina and Perović, Milan and Lačković, Milena and Petrović, Nina and Isenović, Esma R.",
year = "2018",
abstract = "Purpose: This study aimed to investigate in vivo effects of estradiol on the regulation of hepatic inducible nitric oxide synthase (iNOS) expression in the high fat (HF) diet-induced obesity. Also, we aimed to investigate whether activation of the extracellular signal-regulated kinase (ERK1/2), adenosine monophosphate-activated protein kinase (AMPK), Src kinase, and miR-221 is involved in estradiol-mediated regulation of iNOS in the liver of obese male Wistar rats. Male Wistar rats were fed a standard laboratory diet or a HF diet for 10 weeks. Half of HF rats were treated with estradiol intraperitoneally (40 μg/kg), whereas the other half were placebo-treated 24 H before euthanasia. Results show that estradiol treatment of HF rats decreased hepatic iNOS mRNA (P < 0.05) and protein expression (P < 0.01), the protein levels of p65 subunit of nuclear factor κB (P < 0.05) and ERα (P < 0.05), ERK1/2 phosphorylation (P < 0.001), and ERα/Src kinase association (P < 0.05). By contrast, hepatic Src protein level (P < 0.05), AMPKα phosphorylation (P < 0.05), and miR-221 expression (P < 0.05) were increased in HF rats after estradiol treatment. Our results indicate that estradiol in vivo regulates hepatic iNOS expression in obese rats via molecular mechanisms involving ERK1/2, AMPK, Src, and miR-221 signaling. © 2018 International Union of Biochemistry and Molecular Biology, Inc.",
journal = "Biotechnology and Applied Biochemistry",
title = "Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221",
volume = "65",
number = "6",
pages = "797-806",
doi = "10.1002/bab.1680"
}

Panić, A., Stanimirović, J., Obradović, M. M., Sudar-Milovanović, E., Perović, M., Lačković, M., Petrović, N.,& Isenović, E. R.. (2018). Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221. in Biotechnology and Applied Biochemistry, 65(6), 797-806.
https://doi.org/10.1002/bab.1680

Panić A, Stanimirović J, Obradović MM, Sudar-Milovanović E, Perović M, Lačković M, Petrović N, Isenović ER. Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221. in Biotechnology and Applied Biochemistry. 2018;65(6):797-806.
doi:10.1002/bab.1680 .

Panić, Anastasija, Stanimirović, Julijana, Obradović, Milan M., Sudar-Milovanović, Emina, Perović, Milan, Lačković, Milena, Petrović, Nina, Isenović, Esma R., "Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221" in Biotechnology and Applied Biochemistry, 65, no. 6 (2018):797-806,
https://doi.org/10.1002/bab.1680 . .

TY  - JOUR
AU  - Perović, Milan
AU  - Obradović, Milan M.
AU  - Resanović, Ivana
AU  - Isenović, Esma R.
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7749
T2  - Current Vascular Pharmacology
T1  - Relationship between Vitamin D and Metalloproteinases (MMPs) in Acute Myocardial Infarction (AMI) (Editorial)
VL  - 16
IS  - 4
SP  - 361
EP  - 362
DO  - 10.2174/1570161115999171004111230
ER  - 

@article{
author = "Perović, Milan and Obradović, Milan M. and Resanović, Ivana and Isenović, Esma R.",
year = "2018",
journal = "Current Vascular Pharmacology",
title = "Relationship between Vitamin D and Metalloproteinases (MMPs) in Acute Myocardial Infarction (AMI) (Editorial)",
volume = "16",
number = "4",
pages = "361-362",
doi = "10.2174/1570161115999171004111230"
}

Perović, M., Obradović, M. M., Resanović, I.,& Isenović, E. R.. (2018). Relationship between Vitamin D and Metalloproteinases (MMPs) in Acute Myocardial Infarction (AMI) (Editorial). in Current Vascular Pharmacology, 16(4), 361-362.
https://doi.org/10.2174/1570161115999171004111230

Perović M, Obradović MM, Resanović I, Isenović ER. Relationship between Vitamin D and Metalloproteinases (MMPs) in Acute Myocardial Infarction (AMI) (Editorial). in Current Vascular Pharmacology. 2018;16(4):361-362.
doi:10.2174/1570161115999171004111230 .

Perović, Milan, Obradović, Milan M., Resanović, Ivana, Isenović, Esma R., "Relationship between Vitamin D and Metalloproteinases (MMPs) in Acute Myocardial Infarction (AMI) (Editorial)" in Current Vascular Pharmacology, 16, no. 4 (2018):361-362,
https://doi.org/10.2174/1570161115999171004111230 . .

TY  - CHAP
AU  - Obradović, Milan M.
AU  - Zarić, Božidarka
AU  - Sudar-Milovanović, Emina
AU  - Perović, Milan
AU  - Resanović, Ivana
AU  - Gluvić, Zoran
AU  - Isenović, Esma R.
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8014
AB  - Nitric oxide (NO) is a free radical which, in reactions with various molecules causes multiple biological effects. NO is exceptionally regulated and extends to almost every cell type and function within circulation. Generation and actions of NO are regulated by various hormones under physiological and pathophysiological conditions. Nitric oxide synthases (NOS) are the enzymes responsible for NO generation. In mammals, neuronal NOS (nNOS) and endothelial NOS (eNOS) are constitutively expressed, while inducible NOS (iNOS) mediate in immune defense. Altered NO level is associated with obesity, insulin resistance (IR), diabetes and cardiovascular diseases (CVD). Disturbances in eNOS and iNOS regulation accompany multiple changes in endothelial function and contribute to development of CVD. Furthermore, key step in initiation and progression of atherosclerosis is reduction in bioactivity of endothelial cell-derived NO. Here we shall focus on recent literature data related to the role of eNOS and iNOS in physiological and pathophysiological conditions. © 2018 Nova Science Publishers, Inc. All rights reserved.
PB  - Nova Science Publishers
T2  - Horizons in World Cardiovascular Research
T1  - The role of eNOS and iNOS in pathophysiological conditions
VL  - 15
SP  - 65
EP  - 102
UR  - https://hdl.handle.net/21.15107/rcub_vinar_8014
ER  - 

@inbook{
author = "Obradović, Milan M. and Zarić, Božidarka and Sudar-Milovanović, Emina and Perović, Milan and Resanović, Ivana and Gluvić, Zoran and Isenović, Esma R.",
year = "2018",
abstract = "Nitric oxide (NO) is a free radical which, in reactions with various molecules causes multiple biological effects. NO is exceptionally regulated and extends to almost every cell type and function within circulation. Generation and actions of NO are regulated by various hormones under physiological and pathophysiological conditions. Nitric oxide synthases (NOS) are the enzymes responsible for NO generation. In mammals, neuronal NOS (nNOS) and endothelial NOS (eNOS) are constitutively expressed, while inducible NOS (iNOS) mediate in immune defense. Altered NO level is associated with obesity, insulin resistance (IR), diabetes and cardiovascular diseases (CVD). Disturbances in eNOS and iNOS regulation accompany multiple changes in endothelial function and contribute to development of CVD. Furthermore, key step in initiation and progression of atherosclerosis is reduction in bioactivity of endothelial cell-derived NO. Here we shall focus on recent literature data related to the role of eNOS and iNOS in physiological and pathophysiological conditions. © 2018 Nova Science Publishers, Inc. All rights reserved.",
publisher = "Nova Science Publishers",
journal = "Horizons in World Cardiovascular Research",
booktitle = "The role of eNOS and iNOS in pathophysiological conditions",
volume = "15",
pages = "65-102",
url = "https://hdl.handle.net/21.15107/rcub_vinar_8014"
}

Obradović, M. M., Zarić, B., Sudar-Milovanović, E., Perović, M., Resanović, I., Gluvić, Z.,& Isenović, E. R.. (2018). The role of eNOS and iNOS in pathophysiological conditions. in Horizons in World Cardiovascular Research
Nova Science Publishers., 15, 65-102.
https://hdl.handle.net/21.15107/rcub_vinar_8014

Obradović MM, Zarić B, Sudar-Milovanović E, Perović M, Resanović I, Gluvić Z, Isenović ER. The role of eNOS and iNOS in pathophysiological conditions. in Horizons in World Cardiovascular Research. 2018;15:65-102.
https://hdl.handle.net/21.15107/rcub_vinar_8014 .

Obradović, Milan M., Zarić, Božidarka, Sudar-Milovanović, Emina, Perović, Milan, Resanović, Ivana, Gluvić, Zoran, Isenović, Esma R., "The role of eNOS and iNOS in pathophysiological conditions" in Horizons in World Cardiovascular Research, 15 (2018):65-102,
https://hdl.handle.net/21.15107/rcub_vinar_8014 .

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Zarić, Božidarka
AU  - Sudar-Milovanović, Emina
AU  - Ilinčić, Branislava
AU  - Stokić, Edita
AU  - Perović, Milan
AU  - Isenović, Esma R.
PY  - 2018
UR  - http://www.eurekaselect.com/158061/article
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7916
AB  - Despite the intensive research and progress in modern pharmacotherapy, hypercholesterolemia and related cardiovascular complications remain one of the leading causes of mortality and disability in the modern world. A significant contribution to the treatment of hypercholesterolemia was made by the discovery of proprotein convertase subtilisin/kexin type 9 (PCSK9). This enzyme is responsible for the degradation of the low-density lipoprotein (LDL) receptor (LDLR) found at the surface of the plasma membrane in the liver and directly associated with serum LDL level. Limitations in standard therapy used in the treatment of lipid disorders have led to the development of new drugs, such as an inhibitor of PCSK9. Over the past years, the greatest achievement in discovering the PCSK9 inhibitor was made by designing monoclonal antibodies that disable PCSK9 to bind LDLR and RNA interference to reduce PCSK9 production, but one of the main disadvantages is costeffectiveness. In this review, we will summarize the most recent findings of basic and clinical studies which focus on PCSK9 function, regulation and therapeutic target for the treatment of hypercholesterolemia and associated cardiovascular diseases.
T2  - Current Drug Targets
T1  - PCSK9 and Hypercholesterolemia: Therapeutic Approach
VL  - 19
IS  - 9
SP  - 1058
EP  - 1067
DO  - 10.2174/1389450119666171205101401
ER  - 

@article{
author = "Obradović, Milan M. and Zarić, Božidarka and Sudar-Milovanović, Emina and Ilinčić, Branislava and Stokić, Edita and Perović, Milan and Isenović, Esma R.",
year = "2018",
abstract = "Despite the intensive research and progress in modern pharmacotherapy, hypercholesterolemia and related cardiovascular complications remain one of the leading causes of mortality and disability in the modern world. A significant contribution to the treatment of hypercholesterolemia was made by the discovery of proprotein convertase subtilisin/kexin type 9 (PCSK9). This enzyme is responsible for the degradation of the low-density lipoprotein (LDL) receptor (LDLR) found at the surface of the plasma membrane in the liver and directly associated with serum LDL level. Limitations in standard therapy used in the treatment of lipid disorders have led to the development of new drugs, such as an inhibitor of PCSK9. Over the past years, the greatest achievement in discovering the PCSK9 inhibitor was made by designing monoclonal antibodies that disable PCSK9 to bind LDLR and RNA interference to reduce PCSK9 production, but one of the main disadvantages is costeffectiveness. In this review, we will summarize the most recent findings of basic and clinical studies which focus on PCSK9 function, regulation and therapeutic target for the treatment of hypercholesterolemia and associated cardiovascular diseases.",
journal = "Current Drug Targets",
title = "PCSK9 and Hypercholesterolemia: Therapeutic Approach",
volume = "19",
number = "9",
pages = "1058-1067",
doi = "10.2174/1389450119666171205101401"
}

Obradović, M. M., Zarić, B., Sudar-Milovanović, E., Ilinčić, B., Stokić, E., Perović, M.,& Isenović, E. R.. (2018). PCSK9 and Hypercholesterolemia: Therapeutic Approach. in Current Drug Targets, 19(9), 1058-1067.
https://doi.org/10.2174/1389450119666171205101401

Obradović MM, Zarić B, Sudar-Milovanović E, Ilinčić B, Stokić E, Perović M, Isenović ER. PCSK9 and Hypercholesterolemia: Therapeutic Approach. in Current Drug Targets. 2018;19(9):1058-1067.
doi:10.2174/1389450119666171205101401 .

Obradović, Milan M., Zarić, Božidarka, Sudar-Milovanović, Emina, Ilinčić, Branislava, Stokić, Edita, Perović, Milan, Isenović, Esma R., "PCSK9 and Hypercholesterolemia: Therapeutic Approach" in Current Drug Targets, 19, no. 9 (2018):1058-1067,
https://doi.org/10.2174/1389450119666171205101401 . .

TY  - JOUR
AU  - Perović, Milan
AU  - Gojnic, Miroslava
AU  - Arsić, Biljana
AU  - Pantic, Igor
AU  - Stefanovic, Tomislav
AU  - Kovacevic, Gordana
AU  - Kovacevic, Milica
AU  - Garalejic, Eliana
AU  - Dugalic, Stefan
AU  - Radakovic, Jovana
AU  - Babic, Uros
AU  - Isenović, Esma R.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/603
AB  - BackgroundThe aim of the present study was to investigate the relationship between mid-trimester ultrasound fetal liver length (FLL) and gestational diabetes mellitus (GDM) in a high-risk population. MethodsA prospective study was performed in 331 women with singleton pregnancies who were at high risk of GDM and were undergoing a mid-trimester ultrasound examination. The ultrasound scan at 23 weeks gestation was followed by a 100-g oral glucose tolerance test (OGTT) at 24 weeks gestation. Correlations between FLL and OGTT results at different time points were tested. Receiver operating characteristic (ROC) analysis of FLL as a potential prognostic factor for GDM was also performed. ResultsIn GDM patients, there was a significant positive correlation (P LT 0.01) between FLL and OGTT glycemia immediately before and 60, 120, and 180min after glucose intake. Mean FLL in GDM was significantly higher than in healthy subjects (41.04 vs 31.09mm, respectively; P LT 0.001). When tested as a potential prognostic factor for GDM, fetal liver measurements showed excellent diagnostic performance. The ROC analysis established a cut-off value of FLL of 39mm for the prediction GDM, with sensitivity of 71.76%, specificity 97.56%, positive predictive value 91.0%, and negative predictive value 90.9%. The usefulness of FLL measurements was supported by a high area under the ROC curve (90.5%). ConclusionIn conclusion, there is a strong correlation between FLL and OGTT results, with FLL possibly serving as a valid marker for the prediction of GDM in high-risk populations.
T2  - Journal of Diabetes
T1  - Relationship between mid-trimester ultrasound fetal liver length measurements and gestational diabetes mellitus
VL  - 7
IS  - 4
SP  - 497
EP  - 505
DO  - 10.1111/1753-0407.12207
ER  - 

@article{
author = "Perović, Milan and Gojnic, Miroslava and Arsić, Biljana and Pantic, Igor and Stefanovic, Tomislav and Kovacevic, Gordana and Kovacevic, Milica and Garalejic, Eliana and Dugalic, Stefan and Radakovic, Jovana and Babic, Uros and Isenović, Esma R.",
year = "2015",
abstract = "BackgroundThe aim of the present study was to investigate the relationship between mid-trimester ultrasound fetal liver length (FLL) and gestational diabetes mellitus (GDM) in a high-risk population. MethodsA prospective study was performed in 331 women with singleton pregnancies who were at high risk of GDM and were undergoing a mid-trimester ultrasound examination. The ultrasound scan at 23 weeks gestation was followed by a 100-g oral glucose tolerance test (OGTT) at 24 weeks gestation. Correlations between FLL and OGTT results at different time points were tested. Receiver operating characteristic (ROC) analysis of FLL as a potential prognostic factor for GDM was also performed. ResultsIn GDM patients, there was a significant positive correlation (P LT 0.01) between FLL and OGTT glycemia immediately before and 60, 120, and 180min after glucose intake. Mean FLL in GDM was significantly higher than in healthy subjects (41.04 vs 31.09mm, respectively; P LT 0.001). When tested as a potential prognostic factor for GDM, fetal liver measurements showed excellent diagnostic performance. The ROC analysis established a cut-off value of FLL of 39mm for the prediction GDM, with sensitivity of 71.76%, specificity 97.56%, positive predictive value 91.0%, and negative predictive value 90.9%. The usefulness of FLL measurements was supported by a high area under the ROC curve (90.5%). ConclusionIn conclusion, there is a strong correlation between FLL and OGTT results, with FLL possibly serving as a valid marker for the prediction of GDM in high-risk populations.",
journal = "Journal of Diabetes",
title = "Relationship between mid-trimester ultrasound fetal liver length measurements and gestational diabetes mellitus",
volume = "7",
number = "4",
pages = "497-505",
doi = "10.1111/1753-0407.12207"
}

Perović, M., Gojnic, M., Arsić, B., Pantic, I., Stefanovic, T., Kovacevic, G., Kovacevic, M., Garalejic, E., Dugalic, S., Radakovic, J., Babic, U.,& Isenović, E. R.. (2015). Relationship between mid-trimester ultrasound fetal liver length measurements and gestational diabetes mellitus. in Journal of Diabetes, 7(4), 497-505.
https://doi.org/10.1111/1753-0407.12207

Perović M, Gojnic M, Arsić B, Pantic I, Stefanovic T, Kovacevic G, Kovacevic M, Garalejic E, Dugalic S, Radakovic J, Babic U, Isenović ER. Relationship between mid-trimester ultrasound fetal liver length measurements and gestational diabetes mellitus. in Journal of Diabetes. 2015;7(4):497-505.
doi:10.1111/1753-0407.12207 .

Perović, Milan, Gojnic, Miroslava, Arsić, Biljana, Pantic, Igor, Stefanovic, Tomislav, Kovacevic, Gordana, Kovacevic, Milica, Garalejic, Eliana, Dugalic, Stefan, Radakovic, Jovana, Babic, Uros, Isenović, Esma R., "Relationship between mid-trimester ultrasound fetal liver length measurements and gestational diabetes mellitus" in Journal of Diabetes, 7, no. 4 (2015):497-505,
https://doi.org/10.1111/1753-0407.12207 . .

TY  - JOUR
AU  - Resanović, Ivana
AU  - Rizzo, Manfredi
AU  - Zafirović, Sonja
AU  - Bjelogrlic, P.
AU  - Perović, Milan
AU  - Savić, K.
AU  - Patti, A. M.
AU  - Isenović, Esma R.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5680
AB  - Estrogens are secreted primarily by the ovaries and placenta, by the testes in men and also produced by peripheral steroidogenic conversion. The 3 major naturally occurring estrogens are: 17 beta-estradiol (E-2), estrone and estriol, of which E-2 is the predominant and most active. The actions of E-2 are mediated by at least 3 different receptors - the classical ERs (ER alpha and ER beta) and G-protein coupled receptor 30 (GPR30). E-2 signaling in cardiomyocytes involves ER alpha- and ER beta-independent pathways, and treatment with the E-2 receptor antagonists (Selective Estrogen Receptor Modulators- SERMs), which are agonists of GPR30, inhibits cardiac cell growth. Effects of E-2 in preventing endothelial dysfunction, a prerequisite of atherosclerosis, are well recognized. Atherosclerosis involves interaction between the cells of the arterial wall endothelial cells (EC) and vascular smooth muscle cell (VSMC), as well as migration of macrophages into wall tunica media. It is predominantly developed at sites with abnormally high shear stress, such as bifurcations or branching of arteries, initiated by an injury to the endothelium and exposure to atherogenic lipids and toxins, such as those contained in tobacco smoke or infectious agents. Animal studies have shown effects of E-2 in preventing atherosclerosis, inflammation and endothelial or vascular dysfunction. Gender differences along this pathogenic pathway have been also described. We review the data from the available animal and human studies, which focus on anti-atherogenic effects of E-2. These studies represent evidence, albeit indirect, for an inhibitory effect of E-2 on the progression of coronary artery atherosclerosis.
T2  - Hormone and Metabolic Research
T1  - Anti-atherogenic Effects of 17 beta-Estradiol
VL  - 45
IS  - 10
SP  - 701
EP  - 708
DO  - 10.1055/s-0033-1343478
ER  - 

@article{
author = "Resanović, Ivana and Rizzo, Manfredi and Zafirović, Sonja and Bjelogrlic, P. and Perović, Milan and Savić, K. and Patti, A. M. and Isenović, Esma R.",
year = "2013",
abstract = "Estrogens are secreted primarily by the ovaries and placenta, by the testes in men and also produced by peripheral steroidogenic conversion. The 3 major naturally occurring estrogens are: 17 beta-estradiol (E-2), estrone and estriol, of which E-2 is the predominant and most active. The actions of E-2 are mediated by at least 3 different receptors - the classical ERs (ER alpha and ER beta) and G-protein coupled receptor 30 (GPR30). E-2 signaling in cardiomyocytes involves ER alpha- and ER beta-independent pathways, and treatment with the E-2 receptor antagonists (Selective Estrogen Receptor Modulators- SERMs), which are agonists of GPR30, inhibits cardiac cell growth. Effects of E-2 in preventing endothelial dysfunction, a prerequisite of atherosclerosis, are well recognized. Atherosclerosis involves interaction between the cells of the arterial wall endothelial cells (EC) and vascular smooth muscle cell (VSMC), as well as migration of macrophages into wall tunica media. It is predominantly developed at sites with abnormally high shear stress, such as bifurcations or branching of arteries, initiated by an injury to the endothelium and exposure to atherogenic lipids and toxins, such as those contained in tobacco smoke or infectious agents. Animal studies have shown effects of E-2 in preventing atherosclerosis, inflammation and endothelial or vascular dysfunction. Gender differences along this pathogenic pathway have been also described. We review the data from the available animal and human studies, which focus on anti-atherogenic effects of E-2. These studies represent evidence, albeit indirect, for an inhibitory effect of E-2 on the progression of coronary artery atherosclerosis.",
journal = "Hormone and Metabolic Research",
title = "Anti-atherogenic Effects of 17 beta-Estradiol",
volume = "45",
number = "10",
pages = "701-708",
doi = "10.1055/s-0033-1343478"
}

Resanović, I., Rizzo, M., Zafirović, S., Bjelogrlic, P., Perović, M., Savić, K., Patti, A. M.,& Isenović, E. R.. (2013). Anti-atherogenic Effects of 17 beta-Estradiol. in Hormone and Metabolic Research, 45(10), 701-708.
https://doi.org/10.1055/s-0033-1343478

Resanović I, Rizzo M, Zafirović S, Bjelogrlic P, Perović M, Savić K, Patti AM, Isenović ER. Anti-atherogenic Effects of 17 beta-Estradiol. in Hormone and Metabolic Research. 2013;45(10):701-708.
doi:10.1055/s-0033-1343478 .

Resanović, Ivana, Rizzo, Manfredi, Zafirović, Sonja, Bjelogrlic, P., Perović, Milan, Savić, K., Patti, A. M., Isenović, Esma R., "Anti-atherogenic Effects of 17 beta-Estradiol" in Hormone and Metabolic Research, 45, no. 10 (2013):701-708,
https://doi.org/10.1055/s-0033-1343478 . .