TY  - JOUR
AU  - Uskoković, Vuk
AU  - Huynh, Eric
AU  - Tang, Sean
AU  - Jovanović, Sonja
AU  - Wu, Victoria
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0927776519303182
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8202
AB  - Understanding the difference in physicochemical properties and biological response between colloidal and powder formulations of identical materials is important before the given materials are used in a medical milieu. In this study we compared a set of biological effects of colloidal and powder formulations of composite nanoparticles comprising superparamagnetic iron oxide cores and silicate/carbon shells. Magnetic dipole interaction between adjacent nanoparticles was more pronounced in their powders than in their colloidal formulations. Nanoparticles delivered as powders were thus more responsive to the magnetic field, but exhibited reduced uptake in bone and brain cancer cells, including K7M2 osteosarcoma line and U87 and E297 glioblastoma lines. Specifically, while the alternate magnetic field elicited a more rapid heat generation in cell culture media supplemented with the magnetic powders, the nanoparticles dispersed in the same media were uptaken by the cancer cells more copiously. The cellular uptake proved to be more crucial in defining the effect on cell survival, given that suspended formulations elicited a greater degree of cancer cell death in the magnetic field compared to the powder-containing formulations. Because of this effect, colloidal formulations were able to target cancer cells more effectively than the powders: they reduced the viability of all three tested cancer cell lines to a significantly greater degree that the viability of the normal, MDCK-MDR1 cell line. It is concluded that better uptake profile can make up for the lower heating rate in the AC field and lead to a more effective magnetic hyperthermia therapy. These results also demonstrate that the direct delivery of ferrofluids is more optimal than the administration of their constitutive particles as powders. © 2019 Elsevier B.V.
T2  - Colloids and Surfaces. B: Biointerfaces
T1  - Colloids or powders: Which nanoparticle formulations do cells like more?
VL  - 181
SP  - 39
EP  - 47
DO  - 10.1016/j.colsurfb.2019.05.019
ER  - 

@article{
author = "Uskoković, Vuk and Huynh, Eric and Tang, Sean and Jovanović, Sonja and Wu, Victoria",
year = "2019",
abstract = "Understanding the difference in physicochemical properties and biological response between colloidal and powder formulations of identical materials is important before the given materials are used in a medical milieu. In this study we compared a set of biological effects of colloidal and powder formulations of composite nanoparticles comprising superparamagnetic iron oxide cores and silicate/carbon shells. Magnetic dipole interaction between adjacent nanoparticles was more pronounced in their powders than in their colloidal formulations. Nanoparticles delivered as powders were thus more responsive to the magnetic field, but exhibited reduced uptake in bone and brain cancer cells, including K7M2 osteosarcoma line and U87 and E297 glioblastoma lines. Specifically, while the alternate magnetic field elicited a more rapid heat generation in cell culture media supplemented with the magnetic powders, the nanoparticles dispersed in the same media were uptaken by the cancer cells more copiously. The cellular uptake proved to be more crucial in defining the effect on cell survival, given that suspended formulations elicited a greater degree of cancer cell death in the magnetic field compared to the powder-containing formulations. Because of this effect, colloidal formulations were able to target cancer cells more effectively than the powders: they reduced the viability of all three tested cancer cell lines to a significantly greater degree that the viability of the normal, MDCK-MDR1 cell line. It is concluded that better uptake profile can make up for the lower heating rate in the AC field and lead to a more effective magnetic hyperthermia therapy. These results also demonstrate that the direct delivery of ferrofluids is more optimal than the administration of their constitutive particles as powders. © 2019 Elsevier B.V.",
journal = "Colloids and Surfaces. B: Biointerfaces",
title = "Colloids or powders: Which nanoparticle formulations do cells like more?",
volume = "181",
pages = "39-47",
doi = "10.1016/j.colsurfb.2019.05.019"
}

Uskoković, V., Huynh, E., Tang, S., Jovanović, S.,& Wu, V.. (2019). Colloids or powders: Which nanoparticle formulations do cells like more?. in Colloids and Surfaces. B: Biointerfaces, 181, 39-47.
https://doi.org/10.1016/j.colsurfb.2019.05.019

Uskoković V, Huynh E, Tang S, Jovanović S, Wu V. Colloids or powders: Which nanoparticle formulations do cells like more?. in Colloids and Surfaces. B: Biointerfaces. 2019;181:39-47.
doi:10.1016/j.colsurfb.2019.05.019 .

Uskoković, Vuk, Huynh, Eric, Tang, Sean, Jovanović, Sonja, Wu, Victoria, "Colloids or powders: Which nanoparticle formulations do cells like more?" in Colloids and Surfaces. B: Biointerfaces, 181 (2019):39-47,
https://doi.org/10.1016/j.colsurfb.2019.05.019 . .

TY  - JOUR
AU  - Stojanović, Zoran S.
AU  - Ignjatović, Nenad L.
AU  - Wu, Victoria
AU  - Žunič, Vojka
AU  - Veselinović, Ljiljana M.
AU  - Škapin, Srečo Davor
AU  - Miljković, Miroslav
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7575
AB  - Recent developments in bone tissue engineering have led to an increased interest in one-dimensional (1D) hydroxyapatite (HA) nano- and micro-structures such as wires, ribbons and tubes. They have been proposed for use as cell substrates, reinforcing phases in composites and carriers for biologically active substances. Here we demonstrate the synthesis of 1D HA structures using an optimized, urea-assisted, high-yield hydrothermal batch process. The one-pot process, yielding HA structures composed of bundles of ribbons and wires, was typified by the simultaneous occurrence of a multitude of intermediate reactions, failing to meet the uniformity criteria over particle morphology and size. To overcome these issues, the preparation procedure was divided to two stages: dicalcium phosphate platelets synthesized in the first step were used as a precursor for the synthesis of 1D HA in the second stage. Despite the elongated particle morphologies, both the precursor and the final product exhibited excellent biocompatibility and caused no reduction of viability when tested against osteoblastic MC3T3-E1 cells in 2D culture up to the concentration of 2.6 mg/cm2. X-ray powder diffraction combined with a range of electron microscopies and laser diffraction analyses was used to elucidate the formation mechanism and the microstructure of the final particles. The two-step synthesis involved a more direct transformation of DCP to 1D HA with the average diameter of 37 nm and the aspect ratio exceeding 100:1. The comparison of crystalline domain sizes along different crystallographic directions showed no signs of significant anisotropy, while indicating that individual nanowires are ordered in bundles in the b crystallographic direction of the P63/m space group of HA. Intermediate processes, e.g., dehydration of dicalcium phosphate, are critical for the formation of 1D HA alongside other key aspects of this phase transformation, it must be investigated in more detail in the continuous design of smart HA micro- and nano-structures with advanced therapeutic potentials.
T2  - Materials Science and Engineering: C
T1  - Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies
VL  - 68
SP  - 746
EP  - 757
DO  - 10.1016/j.msec.2016.06.047
ER  - 

@article{
author = "Stojanović, Zoran S. and Ignjatović, Nenad L. and Wu, Victoria and Žunič, Vojka and Veselinović, Ljiljana M. and Škapin, Srečo Davor and Miljković, Miroslav and Uskoković, Vuk and Uskoković, Dragan",
year = "2016",
abstract = "Recent developments in bone tissue engineering have led to an increased interest in one-dimensional (1D) hydroxyapatite (HA) nano- and micro-structures such as wires, ribbons and tubes. They have been proposed for use as cell substrates, reinforcing phases in composites and carriers for biologically active substances. Here we demonstrate the synthesis of 1D HA structures using an optimized, urea-assisted, high-yield hydrothermal batch process. The one-pot process, yielding HA structures composed of bundles of ribbons and wires, was typified by the simultaneous occurrence of a multitude of intermediate reactions, failing to meet the uniformity criteria over particle morphology and size. To overcome these issues, the preparation procedure was divided to two stages: dicalcium phosphate platelets synthesized in the first step were used as a precursor for the synthesis of 1D HA in the second stage. Despite the elongated particle morphologies, both the precursor and the final product exhibited excellent biocompatibility and caused no reduction of viability when tested against osteoblastic MC3T3-E1 cells in 2D culture up to the concentration of 2.6 mg/cm2. X-ray powder diffraction combined with a range of electron microscopies and laser diffraction analyses was used to elucidate the formation mechanism and the microstructure of the final particles. The two-step synthesis involved a more direct transformation of DCP to 1D HA with the average diameter of 37 nm and the aspect ratio exceeding 100:1. The comparison of crystalline domain sizes along different crystallographic directions showed no signs of significant anisotropy, while indicating that individual nanowires are ordered in bundles in the b crystallographic direction of the P63/m space group of HA. Intermediate processes, e.g., dehydration of dicalcium phosphate, are critical for the formation of 1D HA alongside other key aspects of this phase transformation, it must be investigated in more detail in the continuous design of smart HA micro- and nano-structures with advanced therapeutic potentials.",
journal = "Materials Science and Engineering: C",
title = "Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies",
volume = "68",
pages = "746-757",
doi = "10.1016/j.msec.2016.06.047"
}

Stojanović, Z. S., Ignjatović, N. L., Wu, V., Žunič, V., Veselinović, L. M., Škapin, S. D., Miljković, M., Uskoković, V.,& Uskoković, D.. (2016). Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies. in Materials Science and Engineering: C, 68, 746-757.
https://doi.org/10.1016/j.msec.2016.06.047

Stojanović ZS, Ignjatović NL, Wu V, Žunič V, Veselinović LM, Škapin SD, Miljković M, Uskoković V, Uskoković D. Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies. in Materials Science and Engineering: C. 2016;68:746-757.
doi:10.1016/j.msec.2016.06.047 .

Stojanović, Zoran S., Ignjatović, Nenad L., Wu, Victoria, Žunič, Vojka, Veselinović, Ljiljana M., Škapin, Srečo Davor, Miljković, Miroslav, Uskoković, Vuk, Uskoković, Dragan, "Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies" in Materials Science and Engineering: C, 68 (2016):746-757,
https://doi.org/10.1016/j.msec.2016.06.047 . .

TY  - JOUR
AU  - Stojanović, Zoran S.
AU  - Ignjatović, Nenad L.
AU  - Wu, Victoria
AU  - Žunič, Vojka
AU  - Veselinović, Ljiljana M.
AU  - Škapin, Srečo Davor
AU  - Miljković, Miroslav
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7576
AB  - Recent developments in bone tissue engineering have led to an increased interest in one-dimensional (1D) hydroxyapatite (HA) nano- and micro-structures such as wires, ribbons and tubes. They have been proposed for use as cell substrates, reinforcing phases in composites and carriers for biologically active substances. Here we demonstrate the synthesis of 1D HA structures using an optimized, urea-assisted, high-yield hydrothermal batch process. The one-pot process, yielding HA structures composed of bundles of ribbons and wires, was typified by the simultaneous occurrence of a multitude of intermediate reactions, failing to meet the uniformity criteria over particle morphology and size. To overcome these issues, the preparation procedure was divided to two stages: dicalcium phosphate platelets synthesized in the first step were used as a precursor for the synthesis of 1D HA in the second stage. Despite the elongated particle morphologies, both the precursor and the final product exhibited excellent biocompatibility and caused no reduction of viability when tested against osteoblastic MC3T3-E1 cells in 2D culture up to the concentration of 2.6 mg/cm2. X-ray powder diffraction combined with a range of electron microscopies and laser diffraction analyses was used to elucidate the formation mechanism and the microstructure of the final particles. The two-step synthesis involved a more direct transformation of DCP to 1D HA with the average diameter of 37 nm and the aspect ratio exceeding 100:1. The comparison of crystalline domain sizes along different crystallographic directions showed no signs of significant anisotropy, while indicating that individual nanowires are ordered in bundles in the b crystallographic direction of the P63/m space group of HA. Intermediate processes, e.g., dehydration of dicalcium phosphate, are critical for the formation of 1D HA alongside other key aspects of this phase transformation, it must be investigated in more detail in the continuous design of smart HA micro- and nano-structures with advanced therapeutic potentials.
PB  - Elsevier
T2  - Materials Science and Engineering: C
T1  - Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies
VL  - 68
SP  - 746
EP  - 757
DO  - 10.1016/j.msec.2016.06.047
ER  - 

@article{
author = "Stojanović, Zoran S. and Ignjatović, Nenad L. and Wu, Victoria and Žunič, Vojka and Veselinović, Ljiljana M. and Škapin, Srečo Davor and Miljković, Miroslav and Uskoković, Vuk and Uskoković, Dragan",
year = "2016",
abstract = "Recent developments in bone tissue engineering have led to an increased interest in one-dimensional (1D) hydroxyapatite (HA) nano- and micro-structures such as wires, ribbons and tubes. They have been proposed for use as cell substrates, reinforcing phases in composites and carriers for biologically active substances. Here we demonstrate the synthesis of 1D HA structures using an optimized, urea-assisted, high-yield hydrothermal batch process. The one-pot process, yielding HA structures composed of bundles of ribbons and wires, was typified by the simultaneous occurrence of a multitude of intermediate reactions, failing to meet the uniformity criteria over particle morphology and size. To overcome these issues, the preparation procedure was divided to two stages: dicalcium phosphate platelets synthesized in the first step were used as a precursor for the synthesis of 1D HA in the second stage. Despite the elongated particle morphologies, both the precursor and the final product exhibited excellent biocompatibility and caused no reduction of viability when tested against osteoblastic MC3T3-E1 cells in 2D culture up to the concentration of 2.6 mg/cm2. X-ray powder diffraction combined with a range of electron microscopies and laser diffraction analyses was used to elucidate the formation mechanism and the microstructure of the final particles. The two-step synthesis involved a more direct transformation of DCP to 1D HA with the average diameter of 37 nm and the aspect ratio exceeding 100:1. The comparison of crystalline domain sizes along different crystallographic directions showed no signs of significant anisotropy, while indicating that individual nanowires are ordered in bundles in the b crystallographic direction of the P63/m space group of HA. Intermediate processes, e.g., dehydration of dicalcium phosphate, are critical for the formation of 1D HA alongside other key aspects of this phase transformation, it must be investigated in more detail in the continuous design of smart HA micro- and nano-structures with advanced therapeutic potentials.",
publisher = "Elsevier",
journal = "Materials Science and Engineering: C",
title = "Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies",
volume = "68",
pages = "746-757",
doi = "10.1016/j.msec.2016.06.047"
}

Stojanović, Z. S., Ignjatović, N. L., Wu, V., Žunič, V., Veselinović, L. M., Škapin, S. D., Miljković, M., Uskoković, V.,& Uskoković, D.. (2016). Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies. in Materials Science and Engineering: C
Elsevier., 68, 746-757.
https://doi.org/10.1016/j.msec.2016.06.047

Stojanović ZS, Ignjatović NL, Wu V, Žunič V, Veselinović LM, Škapin SD, Miljković M, Uskoković V, Uskoković D. Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies. in Materials Science and Engineering: C. 2016;68:746-757.
doi:10.1016/j.msec.2016.06.047 .

Stojanović, Zoran S., Ignjatović, Nenad L., Wu, Victoria, Žunič, Vojka, Veselinović, Ljiljana M., Škapin, Srečo Davor, Miljković, Miroslav, Uskoković, Vuk, Uskoković, Dragan, "Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies" in Materials Science and Engineering: C, 68 (2016):746-757,
https://doi.org/10.1016/j.msec.2016.06.047 . .

TY  - CONF
AU  - Ignjatović, Nenad L.
AU  - Penov Gaši, Katarina
AU  - Wu, Victoria
AU  - Ajduković, Jovana
AU  - Kojić, Vesna V.
AU  - Vasiljević-Radović, Dana
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2016
UR  - http://dais.sanu.ac.rs/123456789/896
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7553
AB  - The applicative potential of synthetic calcium phosphates, especially hydroxyapatite (HAp), has become intensely broadened in the past 10 years, from bone tissue engineering to multiple other fields of biomedicine. Previously we have shown that hydroxyapatite nanoparticles coated with chitosan-poly(D,L)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous administration into mice. For this purpose radioactive 125-Iodine (125I), a low energy gamma emitter, was used to develop a novel in situ method for radiolabeling of particles and investigation of their biodistribution. In this study we utilize an emulsification process and freeze drying to load the composite particles based on hydroxyapatite nanocarrier, chitosane and poly(lactic-co-glycolic acid) with 17β- hydroxy-17α-picolyl-androst-5-en-3β-acetate (A), a chemotherapeutic derivative of androstane. The picolyl androstane derivatives showed high potency in the cell inhibitors of hormonedependent cancers (adenocarcinoma, prostate cancer, cervix carcinoma, colon cancer, etc.). 1H NMR, 13C NMR and high-resolution time-of-flight mass spectrometry (MS) techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The synthesized particles of A-loaded HAp/Ch-PLGA were found to be spherical in shape with a uniform size distribution of d50=168 nm. The release of A from HAp/Ch-PLGA was sustained, with no burst release or plateauing after three weeks. The obtained results of the DET and MTT tests show that the particles of A-loaded HAp/Ch-PLGA exhibit almost three times higher cytotoxicity towards lung adenocarcinoma cells (A549) than towards healthy cells (MRC5), while at the same time allowing twice as fast recovery of healthy cells. We have also analyzed the period of recovery of healthy, as well as cancer cells, following the treatment with A-loaded HAp/Ch-PLGA. After treatment with A-loaded HAp/Ch-PLGA, healthy cells recover twice as fast as the malignant ones. Immunofluorescent staining of primary fibroblasts interacting with HAp/Ch-PLGA and A-HAp/Ch-PLGA particles demonstrates no negative morphological or proliferative effects on cells.
PB  - Belgrade : Materials Research Society of Serbia
C3  - YUCOMAT 2016 : 18th Annual Conference YUCOMAT 2016 : programme and the book of abstracts; September 5-10, Herceg Novi
T1  - Tumor-selective hybrid system based on hydroxyapatite nanocarrier, chitosan, poly(lactic-co-glycolic acid) and androstan derivate
SP  - 27
EP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7553
ER  - 

@conference{
author = "Ignjatović, Nenad L. and Penov Gaši, Katarina and Wu, Victoria and Ajduković, Jovana and Kojić, Vesna V. and Vasiljević-Radović, Dana and Uskoković, Vuk and Uskoković, Dragan",
year = "2016",
abstract = "The applicative potential of synthetic calcium phosphates, especially hydroxyapatite (HAp), has become intensely broadened in the past 10 years, from bone tissue engineering to multiple other fields of biomedicine. Previously we have shown that hydroxyapatite nanoparticles coated with chitosan-poly(D,L)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous administration into mice. For this purpose radioactive 125-Iodine (125I), a low energy gamma emitter, was used to develop a novel in situ method for radiolabeling of particles and investigation of their biodistribution. In this study we utilize an emulsification process and freeze drying to load the composite particles based on hydroxyapatite nanocarrier, chitosane and poly(lactic-co-glycolic acid) with 17β- hydroxy-17α-picolyl-androst-5-en-3β-acetate (A), a chemotherapeutic derivative of androstane. The picolyl androstane derivatives showed high potency in the cell inhibitors of hormonedependent cancers (adenocarcinoma, prostate cancer, cervix carcinoma, colon cancer, etc.). 1H NMR, 13C NMR and high-resolution time-of-flight mass spectrometry (MS) techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The synthesized particles of A-loaded HAp/Ch-PLGA were found to be spherical in shape with a uniform size distribution of d50=168 nm. The release of A from HAp/Ch-PLGA was sustained, with no burst release or plateauing after three weeks. The obtained results of the DET and MTT tests show that the particles of A-loaded HAp/Ch-PLGA exhibit almost three times higher cytotoxicity towards lung adenocarcinoma cells (A549) than towards healthy cells (MRC5), while at the same time allowing twice as fast recovery of healthy cells. We have also analyzed the period of recovery of healthy, as well as cancer cells, following the treatment with A-loaded HAp/Ch-PLGA. After treatment with A-loaded HAp/Ch-PLGA, healthy cells recover twice as fast as the malignant ones. Immunofluorescent staining of primary fibroblasts interacting with HAp/Ch-PLGA and A-HAp/Ch-PLGA particles demonstrates no negative morphological or proliferative effects on cells.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "YUCOMAT 2016 : 18th Annual Conference YUCOMAT 2016 : programme and the book of abstracts; September 5-10, Herceg Novi",
title = "Tumor-selective hybrid system based on hydroxyapatite nanocarrier, chitosan, poly(lactic-co-glycolic acid) and androstan derivate",
pages = "27-27",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7553"
}

Ignjatović, N. L., Penov Gaši, K., Wu, V., Ajduković, J., Kojić, V. V., Vasiljević-Radović, D., Uskoković, V.,& Uskoković, D.. (2016). Tumor-selective hybrid system based on hydroxyapatite nanocarrier, chitosan, poly(lactic-co-glycolic acid) and androstan derivate. in YUCOMAT 2016 : 18th Annual Conference YUCOMAT 2016 : programme and the book of abstracts; September 5-10, Herceg Novi
Belgrade : Materials Research Society of Serbia., 27-27.
https://hdl.handle.net/21.15107/rcub_vinar_7553

Ignjatović NL, Penov Gaši K, Wu V, Ajduković J, Kojić VV, Vasiljević-Radović D, Uskoković V, Uskoković D. Tumor-selective hybrid system based on hydroxyapatite nanocarrier, chitosan, poly(lactic-co-glycolic acid) and androstan derivate. in YUCOMAT 2016 : 18th Annual Conference YUCOMAT 2016 : programme and the book of abstracts; September 5-10, Herceg Novi. 2016;:27-27.
https://hdl.handle.net/21.15107/rcub_vinar_7553 .

Ignjatović, Nenad L., Penov Gaši, Katarina, Wu, Victoria, Ajduković, Jovana, Kojić, Vesna V., Vasiljević-Radović, Dana, Uskoković, Vuk, Uskoković, Dragan, "Tumor-selective hybrid system based on hydroxyapatite nanocarrier, chitosan, poly(lactic-co-glycolic acid) and androstan derivate" in YUCOMAT 2016 : 18th Annual Conference YUCOMAT 2016 : programme and the book of abstracts; September 5-10, Herceg Novi (2016):27-27,
https://hdl.handle.net/21.15107/rcub_vinar_7553 .

TY  - JOUR
AU  - Ignjatović, Nenad L.
AU  - Penov-Gaši, Katarina
AU  - Wu, Victoria
AU  - Ajduković, Jovana
AU  - Kojić, Vesna V.
AU  - Vasiljević-Radović, Dana
AU  - Kuzmanović, Maja D.
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2016
UR  - http://dais.sanu.ac.rs/123456789/15974
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7569
AB  - In an earlier study we demonstrated that hydroxyapatite nanoparticles coated with chitosan-poly(d,l)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous injection into mice. In this study we utilize an emulsification process and freeze drying to load the composite HAp/Ch-PLGA particles with 17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (A), a chemotherapeutic derivative of androstane and a novel compound with a selective anticancer activity against lung cancer cells. 1H NMR and 13C NMR techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The thermogravimetric and differential thermal analyses coupled with mass spectrometry were used to assess the thermal degradation products and properties of A-loaded HAp/Ch-PLGA. The loading efficiency, as indicated by the comparison of enthalpies of phase transitions in pure A and A-loaded HAp/Ch-PLGA, equaled 7.47wt.%. The release of A from HAp/Ch-PLGA was sustained, neither exhibiting a burst release nor plateauing after three weeks. Atomic force microscopy and particle size distribution analyses were used to confirm that the particles were spherical with a uniform size distribution of d50=168nm. In vitro cytotoxicity testing of A-loaded HAp/Ch-PLGA using MTT and trypan blue dye exclusion assays demonstrated that the particles were cytotoxic to the A549 human lung carcinoma cell line (46±2%), while simultaneously preserving high viability (83±3%) of regular MRC5 human lung fibroblasts and causing no harm to primary mouse lung fibroblasts. In conclusion, composite A-loaded HAp/Ch-PLGA particles could be seen as promising drug delivery platforms for selective cancer therapies, targeting malignant cells for destruction, while having a significantly lesser cytotoxic effect on the healthy cells.
PB  - Elsevier
T2  - Colloids and Surfaces. B: Biointerfaces
T1  - Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor
VL  - 148
SP  - 629
EP  - 639
DO  - 10.1016/j.colsurfb.2016.09.041
ER  - 

@article{
author = "Ignjatović, Nenad L. and Penov-Gaši, Katarina and Wu, Victoria and Ajduković, Jovana and Kojić, Vesna V. and Vasiljević-Radović, Dana and Kuzmanović, Maja D. and Uskoković, Vuk and Uskoković, Dragan",
year = "2016",
abstract = "In an earlier study we demonstrated that hydroxyapatite nanoparticles coated with chitosan-poly(d,l)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous injection into mice. In this study we utilize an emulsification process and freeze drying to load the composite HAp/Ch-PLGA particles with 17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (A), a chemotherapeutic derivative of androstane and a novel compound with a selective anticancer activity against lung cancer cells. 1H NMR and 13C NMR techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The thermogravimetric and differential thermal analyses coupled with mass spectrometry were used to assess the thermal degradation products and properties of A-loaded HAp/Ch-PLGA. The loading efficiency, as indicated by the comparison of enthalpies of phase transitions in pure A and A-loaded HAp/Ch-PLGA, equaled 7.47wt.%. The release of A from HAp/Ch-PLGA was sustained, neither exhibiting a burst release nor plateauing after three weeks. Atomic force microscopy and particle size distribution analyses were used to confirm that the particles were spherical with a uniform size distribution of d50=168nm. In vitro cytotoxicity testing of A-loaded HAp/Ch-PLGA using MTT and trypan blue dye exclusion assays demonstrated that the particles were cytotoxic to the A549 human lung carcinoma cell line (46±2%), while simultaneously preserving high viability (83±3%) of regular MRC5 human lung fibroblasts and causing no harm to primary mouse lung fibroblasts. In conclusion, composite A-loaded HAp/Ch-PLGA particles could be seen as promising drug delivery platforms for selective cancer therapies, targeting malignant cells for destruction, while having a significantly lesser cytotoxic effect on the healthy cells.",
publisher = "Elsevier",
journal = "Colloids and Surfaces. B: Biointerfaces",
title = "Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor",
volume = "148",
pages = "629-639",
doi = "10.1016/j.colsurfb.2016.09.041"
}

Ignjatović, N. L., Penov-Gaši, K., Wu, V., Ajduković, J., Kojić, V. V., Vasiljević-Radović, D., Kuzmanović, M. D., Uskoković, V.,& Uskoković, D.. (2016). Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor. in Colloids and Surfaces. B: Biointerfaces
Elsevier., 148, 629-639.
https://doi.org/10.1016/j.colsurfb.2016.09.041

Ignjatović NL, Penov-Gaši K, Wu V, Ajduković J, Kojić VV, Vasiljević-Radović D, Kuzmanović MD, Uskoković V, Uskoković D. Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor. in Colloids and Surfaces. B: Biointerfaces. 2016;148:629-639.
doi:10.1016/j.colsurfb.2016.09.041 .

Ignjatović, Nenad L., Penov-Gaši, Katarina, Wu, Victoria, Ajduković, Jovana, Kojić, Vesna V., Vasiljević-Radović, Dana, Kuzmanović, Maja D., Uskoković, Vuk, Uskoković, Dragan, "Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor" in Colloids and Surfaces. B: Biointerfaces, 148 (2016):629-639,
https://doi.org/10.1016/j.colsurfb.2016.09.041 . .

TY  - JOUR
AU  - Ignjatović, Nenad L.
AU  - Wu, Victoria
AU  - Ajduković, Zorica
AU  - Mihajilov-Krstev, Tatjana
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7570
AB  - Composite biomaterials comprising nanostructured hydroxyapatite (HAp) have an enormous potential for natural bone tissue reparation, filling and augmentation. Chitosan (Ch) as a naturally derived polymer has many physicochemical and biological properties that make it an attractive material for use in bone tissue engineering. On the other hand, poly-D,L-lactide-co-glycolide (PLGA) is a synthetic polymer with a long history of use in sustained drug delivery and tissue engineering. However, while chitosan can disrupt the cell membrane integrity and may induce blood thrombosis, PLGA releases acidic byproducts that may cause tissue inflammation and interfere with the healing process. One of the strategies to improve the biocompatibility of Ch and PLGA is to combine them with compounds that exhibit complementary properties. In this study we present the synthesis and characterization, as well as in vitro and in vivo analyses of a nanoparticulate form of HAp coated with two different polymeric systems: (a) Ch and (b) a Ch-PLGA polymer blend. Solvent/non-solvent precipitation and freeze-drying were used for synthesis and processing, respectively, whereas thermogravimetry coupled with mass spectrometry was used for phase identification purposes in the coating process. HAp/Ch composite particles exhibited the highest antimicrobial activity against all four microbial strains tested in this work, but after the reconstruction of the bone defect they also caused inflammatory reactions in the newly formed tissue where the defect had lain. Coating HAp with a polymeric blend composed of Ch and PLGA led to a decrease in the reactivity and antimicrobial activity of the composite particles, but also to an increase in the quality of the newly formed bone tissue in the reconstructed defect area.
PB  - Elsevier
T2  - Materials Science and Engineering C
T1  - Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues
VL  - 60
SP  - 357
EP  - 364
DO  - 10.1016/j.msec.2015.11.061
ER  - 

@article{
author = "Ignjatović, Nenad L. and Wu, Victoria and Ajduković, Zorica and Mihajilov-Krstev, Tatjana and Uskoković, Vuk and Uskoković, Dragan",
year = "2016",
abstract = "Composite biomaterials comprising nanostructured hydroxyapatite (HAp) have an enormous potential for natural bone tissue reparation, filling and augmentation. Chitosan (Ch) as a naturally derived polymer has many physicochemical and biological properties that make it an attractive material for use in bone tissue engineering. On the other hand, poly-D,L-lactide-co-glycolide (PLGA) is a synthetic polymer with a long history of use in sustained drug delivery and tissue engineering. However, while chitosan can disrupt the cell membrane integrity and may induce blood thrombosis, PLGA releases acidic byproducts that may cause tissue inflammation and interfere with the healing process. One of the strategies to improve the biocompatibility of Ch and PLGA is to combine them with compounds that exhibit complementary properties. In this study we present the synthesis and characterization, as well as in vitro and in vivo analyses of a nanoparticulate form of HAp coated with two different polymeric systems: (a) Ch and (b) a Ch-PLGA polymer blend. Solvent/non-solvent precipitation and freeze-drying were used for synthesis and processing, respectively, whereas thermogravimetry coupled with mass spectrometry was used for phase identification purposes in the coating process. HAp/Ch composite particles exhibited the highest antimicrobial activity against all four microbial strains tested in this work, but after the reconstruction of the bone defect they also caused inflammatory reactions in the newly formed tissue where the defect had lain. Coating HAp with a polymeric blend composed of Ch and PLGA led to a decrease in the reactivity and antimicrobial activity of the composite particles, but also to an increase in the quality of the newly formed bone tissue in the reconstructed defect area.",
publisher = "Elsevier",
journal = "Materials Science and Engineering C",
title = "Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues",
volume = "60",
pages = "357-364",
doi = "10.1016/j.msec.2015.11.061"
}

Ignjatović, N. L., Wu, V., Ajduković, Z., Mihajilov-Krstev, T., Uskoković, V.,& Uskoković, D.. (2016). Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues. in Materials Science and Engineering C
Elsevier., 60, 357-364.
https://doi.org/10.1016/j.msec.2015.11.061

Ignjatović NL, Wu V, Ajduković Z, Mihajilov-Krstev T, Uskoković V, Uskoković D. Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues. in Materials Science and Engineering C. 2016;60:357-364.
doi:10.1016/j.msec.2015.11.061 .

Ignjatović, Nenad L., Wu, Victoria, Ajduković, Zorica, Mihajilov-Krstev, Tatjana, Uskoković, Vuk, Uskoković, Dragan, "Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues" in Materials Science and Engineering C, 60 (2016):357-364,
https://doi.org/10.1016/j.msec.2015.11.061 . .

TY  - JOUR
AU  - Ignjatović, Nenad L.
AU  - Wu, Victoria
AU  - Ajduković, Zorica
AU  - Mihajilov Krstev, Tatjana
AU  - Uskoković, Vuk
AU  - Uskoković, Dragan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7573
AB  - Composite biomaterials comprising nanostructured hydroxyapatite (HAp) have an enormous potential for natural bone tissue reparation, filling and augmentation. Chitosan (Ch) as a naturally derived polymer has many physicochemical and biological properties that make it an attractive material for use in bone tissue engineering. On the other hand, poly-D,L-lactide-co-glycolide (PLGA) is a synthetic polymer with a long history of use in sustained drug delivery and tissue engineering. However, while chitosan can disrupt the cell membrane integrity and may induce blood thrombosis, PLGA releases acidic byproducts that may cause tissue inflammation and interfere with the healing process. One of the strategies to improve the biocompatibility of Ch and PLGA is to combine them with compounds that exhibit complementary properties. In this study we present the synthesis and characterization, as well as in vitro and in vivo analyses of a nanoparticulate form of HAp coated with two different polymeric systems: (a) Ch and (b) a Ch-PLGA polymer blend. Solvent/non-solvent precipitation and freeze-drying were used for synthesis and processing, respectively, whereas thermogravimetry coupled with mass spectrometry was used for phase identification purposes in the coating process. HAp/Ch composite particles exhibited the highest antimicrobial activity against all four microbial strains tested in this work, but after the reconstruction of the bone defect they also caused inflammatory reactions in the newly formed tissue where the defect had lain. Coating HAp with a polymeric blend composed of Ch and PLGA led to a decrease in the reactivity and antimicrobial activity of the composite particles, but also to an increase in the quality of the newly formed bone tissue in the reconstructed defect area.
T2  - Materials science & engineering. C, Materials for biological applications
T1  - Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues
VL  - 60
SP  - 357
EP  - 364
DO  - 10.1016/j.msec.2015.11.061
ER  - 

@article{
author = "Ignjatović, Nenad L. and Wu, Victoria and Ajduković, Zorica and Mihajilov Krstev, Tatjana and Uskoković, Vuk and Uskoković, Dragan",
year = "2016",
abstract = "Composite biomaterials comprising nanostructured hydroxyapatite (HAp) have an enormous potential for natural bone tissue reparation, filling and augmentation. Chitosan (Ch) as a naturally derived polymer has many physicochemical and biological properties that make it an attractive material for use in bone tissue engineering. On the other hand, poly-D,L-lactide-co-glycolide (PLGA) is a synthetic polymer with a long history of use in sustained drug delivery and tissue engineering. However, while chitosan can disrupt the cell membrane integrity and may induce blood thrombosis, PLGA releases acidic byproducts that may cause tissue inflammation and interfere with the healing process. One of the strategies to improve the biocompatibility of Ch and PLGA is to combine them with compounds that exhibit complementary properties. In this study we present the synthesis and characterization, as well as in vitro and in vivo analyses of a nanoparticulate form of HAp coated with two different polymeric systems: (a) Ch and (b) a Ch-PLGA polymer blend. Solvent/non-solvent precipitation and freeze-drying were used for synthesis and processing, respectively, whereas thermogravimetry coupled with mass spectrometry was used for phase identification purposes in the coating process. HAp/Ch composite particles exhibited the highest antimicrobial activity against all four microbial strains tested in this work, but after the reconstruction of the bone defect they also caused inflammatory reactions in the newly formed tissue where the defect had lain. Coating HAp with a polymeric blend composed of Ch and PLGA led to a decrease in the reactivity and antimicrobial activity of the composite particles, but also to an increase in the quality of the newly formed bone tissue in the reconstructed defect area.",
journal = "Materials science & engineering. C, Materials for biological applications",
title = "Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues",
volume = "60",
pages = "357-364",
doi = "10.1016/j.msec.2015.11.061"
}

Ignjatović, N. L., Wu, V., Ajduković, Z., Mihajilov Krstev, T., Uskoković, V.,& Uskoković, D.. (2016). Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues. in Materials science & engineering. C, Materials for biological applications, 60, 357-364.
https://doi.org/10.1016/j.msec.2015.11.061

Ignjatović NL, Wu V, Ajduković Z, Mihajilov Krstev T, Uskoković V, Uskoković D. Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues. in Materials science & engineering. C, Materials for biological applications. 2016;60:357-364.
doi:10.1016/j.msec.2015.11.061 .

Ignjatović, Nenad L., Wu, Victoria, Ajduković, Zorica, Mihajilov Krstev, Tatjana, Uskoković, Vuk, Uskoković, Dragan, "Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues" in Materials science & engineering. C, Materials for biological applications, 60 (2016):357-364,
https://doi.org/10.1016/j.msec.2015.11.061 . .