TY  - CONF
AU  - Tasić, Jelena
AU  - Vidičević Novaković, Sašenka
AU  - Stanojević, Željka
AU  - Paunović, Verica
AU  - Petričević, Saša
AU  - Martinović, Tamara
AU  - Kravić-Stevović, Tamara
AU  - Ćirić, Darko
AU  - Marković, Zoran J.
AU  - Isaković, Aleksandra
AU  - Trajković, Vladimir
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11057
AB  - Background: Experimental autoimmune encephalomyelitis (EAE) is one of the most studied model of neuroinflammation, used to test immunomodulatory and antiinflammatory drugs. Graphene quantum dots (GQD) are oval graphite twodimensional sheets with a diameter <100 nm, one carbon atom thickness, with potential applications in biomedicine. Objective: To investigate the potential protective effect of GQD in EAE model. Methods: Female DA rats were immunized with spinal cord homogenate and Freund’s complete adjuvant. GQD treatment (10 mg/kg, ip) was administrated during the inductive, effector and both phases of a disease. MAP kinase (MAPK) and Akt activity in popliteal lymph nodes (PLN) and CNS was determined by western blot. Quantitative PCR and flow cytometry were used to examine the expression of proinflammatory cytokines and specific transcription factors while infiltration of GQD in cells/tissues was detected by transmission electron microscopy. GQD antiinflamatory/direct cytoprotective effect was analyzed on oligodendrocyte and neuron cell cultures by MTT assay. Data were analized by Mann Whitney test (p<0.05 was considered as statistical significant difference). Results: GQD administration, in all phases of EAE, significantly reduced clinical score of a disease. Clinical improvement correlates with increase in activity of ERK, p38 and Akt that is followed by reduction of Th1 cell response in PLN and infiltrated spinal coard T cells. Due to its capacity to infiltrate cells and tissues, GQD exhibits direct cytoprotective effect on CNS. Additionaly, GQD reduced the expression of proinflammatory cytokines in ConA stimulated lymphocytes. Conclusion: GQD alleviate EAE, through direct cytoprotective effect on CNS and inhibition of Th1 cell response.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Graphene Quantum Dots show protective effect in animal model of neuroinflammation
SP  - 114
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11057
ER  - 

@conference{
author = "Tasić, Jelena and Vidičević Novaković, Sašenka and Stanojević, Željka and Paunović, Verica and Petričević, Saša and Martinović, Tamara and Kravić-Stevović, Tamara and Ćirić, Darko and Marković, Zoran J. and Isaković, Aleksandra and Trajković, Vladimir",
year = "2023",
abstract = "Background: Experimental autoimmune encephalomyelitis (EAE) is one of the most studied model of neuroinflammation, used to test immunomodulatory and antiinflammatory drugs. Graphene quantum dots (GQD) are oval graphite twodimensional sheets with a diameter <100 nm, one carbon atom thickness, with potential applications in biomedicine. Objective: To investigate the potential protective effect of GQD in EAE model. Methods: Female DA rats were immunized with spinal cord homogenate and Freund’s complete adjuvant. GQD treatment (10 mg/kg, ip) was administrated during the inductive, effector and both phases of a disease. MAP kinase (MAPK) and Akt activity in popliteal lymph nodes (PLN) and CNS was determined by western blot. Quantitative PCR and flow cytometry were used to examine the expression of proinflammatory cytokines and specific transcription factors while infiltration of GQD in cells/tissues was detected by transmission electron microscopy. GQD antiinflamatory/direct cytoprotective effect was analyzed on oligodendrocyte and neuron cell cultures by MTT assay. Data were analized by Mann Whitney test (p<0.05 was considered as statistical significant difference). Results: GQD administration, in all phases of EAE, significantly reduced clinical score of a disease. Clinical improvement correlates with increase in activity of ERK, p38 and Akt that is followed by reduction of Th1 cell response in PLN and infiltrated spinal coard T cells. Due to its capacity to infiltrate cells and tissues, GQD exhibits direct cytoprotective effect on CNS. Additionaly, GQD reduced the expression of proinflammatory cytokines in ConA stimulated lymphocytes. Conclusion: GQD alleviate EAE, through direct cytoprotective effect on CNS and inhibition of Th1 cell response.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Graphene Quantum Dots show protective effect in animal model of neuroinflammation",
pages = "114",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11057"
}

Tasić, J., Vidičević Novaković, S., Stanojević, Ž., Paunović, V., Petričević, S., Martinović, T., Kravić-Stevović, T., Ćirić, D., Marković, Z. J., Isaković, A.,& Trajković, V.. (2023). Graphene Quantum Dots show protective effect in animal model of neuroinflammation. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 114.
https://hdl.handle.net/21.15107/rcub_vinar_11057

Tasić J, Vidičević Novaković S, Stanojević Ž, Paunović V, Petričević S, Martinović T, Kravić-Stevović T, Ćirić D, Marković ZJ, Isaković A, Trajković V. Graphene Quantum Dots show protective effect in animal model of neuroinflammation. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:114.
https://hdl.handle.net/21.15107/rcub_vinar_11057 .

Tasić, Jelena, Vidičević Novaković, Sašenka, Stanojević, Željka, Paunović, Verica, Petričević, Saša, Martinović, Tamara, Kravić-Stevović, Tamara, Ćirić, Darko, Marković, Zoran J., Isaković, Aleksandra, Trajković, Vladimir, "Graphene Quantum Dots show protective effect in animal model of neuroinflammation" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):114,
https://hdl.handle.net/21.15107/rcub_vinar_11057 .

TY  - JOUR
AU  - Tošić, Jelena
AU  - Stanojević, Željka
AU  - Vidičević, Sašenka
AU  - Isaković, Aleksandra J.
AU  - Ćirić, Darko
AU  - Martinović, Tamara
AU  - Kravić-Stevović, Tamara K.
AU  - Bumbaširević, Vladimir Ž.
AU  - Paunović, Verica G.
AU  - Jovanović, Svetlana P.
AU  - Todorović-Marković, Biljana
AU  - Marković, Zoran M.
AU  - Danko, Martin
AU  - Mičušik, Matej
AU  - Spitalsky, Zdenko
AU  - Trajković, Vladimir S.
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0028390818308621
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8015
AB  - We investigated the therapeutic capacity of nano-sized graphene sheets, called graphene quantum dots (GQD), in experimental autoimmune encephalomyelitis (EAE), an animal model of immune-mediated central nervous system (CNS) damage. Intraperitoneally administered GQD (10 mg/kg/day) accumulated in the lymph node and CNS cells of Dark Agouti rats in which EAE was induced by immunization with spinal cord homogenate in complete Freund's adjuvant. GQD significantly reduced clinical signs of EAE when applied throughout the course of the disease (day 0–32), while the protection was less pronounced if the treatment was limited to the induction (day 0–7 post-immunization) or effector (from day 8 onwards) phase of the disease. GQD treatment diminished immune infiltration, demyelination, axonal damage, and apoptotic death in the CNS of EAE animals. GQD also reduced the numbers of interferon-γ-expressing T helper (Th)1 cells, as well as the expression of Th1 transcription factor T-bet and proinflammatory cytokines tumor necrosis factor, interleukin-1, and granulocyte-macrophage colony-stimulating factor in the lymph nodes and CNS immune infitrates. The protective effect of GQD in EAE was associated with the activation of p38 and p42/44 mitogen-activated protein kinases (MAPK) and Akt in the lymph nodes and/or CNS. Finally, GQD protected oligodendrocytes and neurons from T cell-mediated damage in the in vitro conditions. Collectively, these data demonstrate the ability of GQD to gain access to both immune and CNS cells during neuroinflammation, and to alleviate immune-mediated CNS damage by modulating MAPK/Akt signaling and encephalitogenic Th1 immune response. © 2018 Elsevier Ltd
T2  - Neuropharmacology
T1  - Graphene quantum dots inhibit T cell-mediated neuroinflammation in rats
VL  - 146
SP  - 95
EP  - 108
DO  - 10.1016/j.neuropharm.2018.11.030
ER  - 

@article{
author = "Tošić, Jelena and Stanojević, Željka and Vidičević, Sašenka and Isaković, Aleksandra J. and Ćirić, Darko and Martinović, Tamara and Kravić-Stevović, Tamara K. and Bumbaširević, Vladimir Ž. and Paunović, Verica G. and Jovanović, Svetlana P. and Todorović-Marković, Biljana and Marković, Zoran M. and Danko, Martin and Mičušik, Matej and Spitalsky, Zdenko and Trajković, Vladimir S.",
year = "2019",
abstract = "We investigated the therapeutic capacity of nano-sized graphene sheets, called graphene quantum dots (GQD), in experimental autoimmune encephalomyelitis (EAE), an animal model of immune-mediated central nervous system (CNS) damage. Intraperitoneally administered GQD (10 mg/kg/day) accumulated in the lymph node and CNS cells of Dark Agouti rats in which EAE was induced by immunization with spinal cord homogenate in complete Freund's adjuvant. GQD significantly reduced clinical signs of EAE when applied throughout the course of the disease (day 0–32), while the protection was less pronounced if the treatment was limited to the induction (day 0–7 post-immunization) or effector (from day 8 onwards) phase of the disease. GQD treatment diminished immune infiltration, demyelination, axonal damage, and apoptotic death in the CNS of EAE animals. GQD also reduced the numbers of interferon-γ-expressing T helper (Th)1 cells, as well as the expression of Th1 transcription factor T-bet and proinflammatory cytokines tumor necrosis factor, interleukin-1, and granulocyte-macrophage colony-stimulating factor in the lymph nodes and CNS immune infitrates. The protective effect of GQD in EAE was associated with the activation of p38 and p42/44 mitogen-activated protein kinases (MAPK) and Akt in the lymph nodes and/or CNS. Finally, GQD protected oligodendrocytes and neurons from T cell-mediated damage in the in vitro conditions. Collectively, these data demonstrate the ability of GQD to gain access to both immune and CNS cells during neuroinflammation, and to alleviate immune-mediated CNS damage by modulating MAPK/Akt signaling and encephalitogenic Th1 immune response. © 2018 Elsevier Ltd",
journal = "Neuropharmacology",
title = "Graphene quantum dots inhibit T cell-mediated neuroinflammation in rats",
volume = "146",
pages = "95-108",
doi = "10.1016/j.neuropharm.2018.11.030"
}

Tošić, J., Stanojević, Ž., Vidičević, S., Isaković, A. J., Ćirić, D., Martinović, T., Kravić-Stevović, T. K., Bumbaširević, V. Ž., Paunović, V. G., Jovanović, S. P., Todorović-Marković, B., Marković, Z. M., Danko, M., Mičušik, M., Spitalsky, Z.,& Trajković, V. S.. (2019). Graphene quantum dots inhibit T cell-mediated neuroinflammation in rats. in Neuropharmacology, 146, 95-108.
https://doi.org/10.1016/j.neuropharm.2018.11.030

Tošić J, Stanojević Ž, Vidičević S, Isaković AJ, Ćirić D, Martinović T, Kravić-Stevović TK, Bumbaširević VŽ, Paunović VG, Jovanović SP, Todorović-Marković B, Marković ZM, Danko M, Mičušik M, Spitalsky Z, Trajković VS. Graphene quantum dots inhibit T cell-mediated neuroinflammation in rats. in Neuropharmacology. 2019;146:95-108.
doi:10.1016/j.neuropharm.2018.11.030 .

Tošić, Jelena, Stanojević, Željka, Vidičević, Sašenka, Isaković, Aleksandra J., Ćirić, Darko, Martinović, Tamara, Kravić-Stevović, Tamara K., Bumbaširević, Vladimir Ž., Paunović, Verica G., Jovanović, Svetlana P., Todorović-Marković, Biljana, Marković, Zoran M., Danko, Martin, Mičušik, Matej, Spitalsky, Zdenko, Trajković, Vladimir S., "Graphene quantum dots inhibit T cell-mediated neuroinflammation in rats" in Neuropharmacology, 146 (2019):95-108,
https://doi.org/10.1016/j.neuropharm.2018.11.030 . .

TY  - CONF
AU  - Tošić, Jelena
AU  - Vidičević, Sašenka
AU  - Stanojević, Željka
AU  - Paunović, Verica G.
AU  - Petricevic, S.
AU  - Martinović, Tamara
AU  - Kravić-Stevović, Tamara K.
AU  - Ćirić, Darko
AU  - Marković, Zoran M.
AU  - Isaković, Aleksandra J.
AU  - Trajković, Vladimir S.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7160
C3  - European Neuropsychopharmacology
T1  - Graphene quantum dots show protective effect on a model of experimental autoimmune encephalomyelitis
VL  - 26
SP  - S211
EP  - S212
DO  - 10.1016/S0924-977X(16)31060-4
ER  - 

@conference{
author = "Tošić, Jelena and Vidičević, Sašenka and Stanojević, Željka and Paunović, Verica G. and Petricevic, S. and Martinović, Tamara and Kravić-Stevović, Tamara K. and Ćirić, Darko and Marković, Zoran M. and Isaković, Aleksandra J. and Trajković, Vladimir S.",
year = "2016",
journal = "European Neuropsychopharmacology",
title = "Graphene quantum dots show protective effect on a model of experimental autoimmune encephalomyelitis",
volume = "26",
pages = "S211-S212",
doi = "10.1016/S0924-977X(16)31060-4"
}

Tošić, J., Vidičević, S., Stanojević, Ž., Paunović, V. G., Petricevic, S., Martinović, T., Kravić-Stevović, T. K., Ćirić, D., Marković, Z. M., Isaković, A. J.,& Trajković, V. S.. (2016). Graphene quantum dots show protective effect on a model of experimental autoimmune encephalomyelitis. in European Neuropsychopharmacology, 26, S211-S212.
https://doi.org/10.1016/S0924-977X(16)31060-4

Tošić J, Vidičević S, Stanojević Ž, Paunović VG, Petricevic S, Martinović T, Kravić-Stevović TK, Ćirić D, Marković ZM, Isaković AJ, Trajković VS. Graphene quantum dots show protective effect on a model of experimental autoimmune encephalomyelitis. in European Neuropsychopharmacology. 2016;26:S211-S212.
doi:10.1016/S0924-977X(16)31060-4 .

Tošić, Jelena, Vidičević, Sašenka, Stanojević, Željka, Paunović, Verica G., Petricevic, S., Martinović, Tamara, Kravić-Stevović, Tamara K., Ćirić, Darko, Marković, Zoran M., Isaković, Aleksandra J., Trajković, Vladimir S., "Graphene quantum dots show protective effect on a model of experimental autoimmune encephalomyelitis" in European Neuropsychopharmacology, 26 (2016):S211-S212,
https://doi.org/10.1016/S0924-977X(16)31060-4 . .