TY  - JOUR
AU  - Algarra, Manuel
AU  - Carrillo, Celia
AU  - Nešić, Maja D.
AU  - Filipović Tričković, Jelena
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Jiménez-Jiménez, José
AU  - Rodriguez-Castellón, Enrique
AU  - Bandosz, Teresa J.
AU  - Petković, Marijana
AU  - Soto, Juan
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12247
AB  - This paper explains the basis for the excitation energy-independent fluorescence emission of biomass-derived carbon dots (CDs) and shows that these CDs have excellent anti-melanoma and anti-metastatic potential. Additionally, we demonstrate that the black carrots´-derived CDs can be exploited as cell cycle-sensing agents, because of the interaction with chromatin material. Besides their optical properties, fluorescent CDs have gained increased attention for image-guided cancer treatment due to their water solubility, environmental friendliness, affordability, ease of synthesis, and primary biocompatibility. CDs have excellent photostability, determined by their precursors and synthesis pathways. In this study, CDs with chemically homogenous surface functional groups were made using a hydrothermal technique from black carrot extract, an anthocyanin-rich substance derived from biomass. The anti-cancer and anti-metastatic properties of black carrot-derived CDs can be attributed to flavylium cations on the surface, spherical forms, and high water dispersibility. Most importantly, these CDs demonstrate a stable emission at a single wavelength, 612 nm, independent of the excitation energy, which we have explained theoretically for the first time.
T2  - Journal of Molecular Structure
T1  - Testing of black-carrots-derived fluorescence imaging and anti-metastatic potential
VL  - 1300
SP  - 137245
DO  - 10.1016/j.molstruc.2023.137245
ER  - 

@article{
author = "Algarra, Manuel and Carrillo, Celia and Nešić, Maja D. and Filipović Tričković, Jelena and Žakula, Jelena and Korićanac, Lela and Jiménez-Jiménez, José and Rodriguez-Castellón, Enrique and Bandosz, Teresa J. and Petković, Marijana and Soto, Juan",
year = "2024",
abstract = "This paper explains the basis for the excitation energy-independent fluorescence emission of biomass-derived carbon dots (CDs) and shows that these CDs have excellent anti-melanoma and anti-metastatic potential. Additionally, we demonstrate that the black carrots´-derived CDs can be exploited as cell cycle-sensing agents, because of the interaction with chromatin material. Besides their optical properties, fluorescent CDs have gained increased attention for image-guided cancer treatment due to their water solubility, environmental friendliness, affordability, ease of synthesis, and primary biocompatibility. CDs have excellent photostability, determined by their precursors and synthesis pathways. In this study, CDs with chemically homogenous surface functional groups were made using a hydrothermal technique from black carrot extract, an anthocyanin-rich substance derived from biomass. The anti-cancer and anti-metastatic properties of black carrot-derived CDs can be attributed to flavylium cations on the surface, spherical forms, and high water dispersibility. Most importantly, these CDs demonstrate a stable emission at a single wavelength, 612 nm, independent of the excitation energy, which we have explained theoretically for the first time.",
journal = "Journal of Molecular Structure",
title = "Testing of black-carrots-derived fluorescence imaging and anti-metastatic potential",
volume = "1300",
pages = "137245",
doi = "10.1016/j.molstruc.2023.137245"
}

Algarra, M., Carrillo, C., Nešić, M. D., Filipović Tričković, J., Žakula, J., Korićanac, L., Jiménez-Jiménez, J., Rodriguez-Castellón, E., Bandosz, T. J., Petković, M.,& Soto, J.. (2024). Testing of black-carrots-derived fluorescence imaging and anti-metastatic potential. in Journal of Molecular Structure, 1300, 137245.
https://doi.org/10.1016/j.molstruc.2023.137245

Algarra M, Carrillo C, Nešić MD, Filipović Tričković J, Žakula J, Korićanac L, Jiménez-Jiménez J, Rodriguez-Castellón E, Bandosz TJ, Petković M, Soto J. Testing of black-carrots-derived fluorescence imaging and anti-metastatic potential. in Journal of Molecular Structure. 2024;1300:137245.
doi:10.1016/j.molstruc.2023.137245 .

Algarra, Manuel, Carrillo, Celia, Nešić, Maja D., Filipović Tričković, Jelena, Žakula, Jelena, Korićanac, Lela, Jiménez-Jiménez, José, Rodriguez-Castellón, Enrique, Bandosz, Teresa J., Petković, Marijana, Soto, Juan, "Testing of black-carrots-derived fluorescence imaging and anti-metastatic potential" in Journal of Molecular Structure, 1300 (2024):137245,
https://doi.org/10.1016/j.molstruc.2023.137245 . .

TY  - CONF
AU  - Ralić, Vanja
AU  - Nešić, Maja
AU  - Abu el Rub, Anamarija
AU  - Dučić, Tanja
AU  - Gemović, Branislava
AU  - Algarra, Manuel
AU  - Stepić, Milutin
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Petković, Marijana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12657
AB  - Nanocomposite system formulated from surface-modified S-doped carbon dot (S-CD) nanoparticle with a potential metallodrug, palladium(II) complex, dichloro(1,2-diaminocyclohexane)palladium(II), [Pd(dach)Cl2] (Pd@S-CD), was investigated as a model system for the treatment of cervical carcinoma (HeLa) cells. To examine the intracellular biochemical effects induced by the Pd@S-CD, we used Synchrotron Radiation-based Fourier-transform infrared spectroscopy (SR FTIR). SR FTIR spectroscopy was employed to investigate the alterations in cellular components’ biochemical composition and secondary structure upon exposure to Pd@S-CD. Spectral analysis, complemented by statistical techniques, revealed changes in biomolecules, lipids, proteins, nucleic acids, and carbohydrates caused by the treatment with Pd@CDs. These results and the increased cytotoxicity of the system demonstrate its high anti-cervical cancer therapeutic potential.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells
SP  - 467
EP  - 470
DO  - 10.46793/ICCBI23.467R
ER  - 

@conference{
author = "Ralić, Vanja and Nešić, Maja and Abu el Rub, Anamarija and Dučić, Tanja and Gemović, Branislava and Algarra, Manuel and Stepić, Milutin and Korićanac, Lela and Žakula, Jelena and Petković, Marijana",
year = "2023",
abstract = "Nanocomposite system formulated from surface-modified S-doped carbon dot (S-CD) nanoparticle with a potential metallodrug, palladium(II) complex, dichloro(1,2-diaminocyclohexane)palladium(II), [Pd(dach)Cl2] (Pd@S-CD), was investigated as a model system for the treatment of cervical carcinoma (HeLa) cells. To examine the intracellular biochemical effects induced by the Pd@S-CD, we used Synchrotron Radiation-based Fourier-transform infrared spectroscopy (SR FTIR). SR FTIR spectroscopy was employed to investigate the alterations in cellular components’ biochemical composition and secondary structure upon exposure to Pd@S-CD. Spectral analysis, complemented by statistical techniques, revealed changes in biomolecules, lipids, proteins, nucleic acids, and carbohydrates caused by the treatment with Pd@CDs. These results and the increased cytotoxicity of the system demonstrate its high anti-cervical cancer therapeutic potential.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells",
pages = "467-470",
doi = "10.46793/ICCBI23.467R"
}

Ralić, V., Nešić, M., Abu el Rub, A., Dučić, T., Gemović, B., Algarra, M., Stepić, M., Korićanac, L., Žakula, J.,& Petković, M.. (2023). SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 467-470.
https://doi.org/10.46793/ICCBI23.467R

Ralić V, Nešić M, Abu el Rub A, Dučić T, Gemović B, Algarra M, Stepić M, Korićanac L, Žakula J, Petković M. SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:467-470.
doi:10.46793/ICCBI23.467R .

Ralić, Vanja, Nešić, Maja, Abu el Rub, Anamarija, Dučić, Tanja, Gemović, Branislava, Algarra, Manuel, Stepić, Milutin, Korićanac, Lela, Žakula, Jelena, Petković, Marijana, "SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):467-470,
https://doi.org/10.46793/ICCBI23.467R . .

TY  - JOUR
AU  - Nešić, Maja D.
AU  - Dučić, Tanja
AU  - Algarra, Manuel
AU  - Popović, Iva A.
AU  - Stepić, Milutin
AU  - Gonçalves, Mara
AU  - Petković, Marijana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10184
AB  - In the last decade, targeting membrane lipids in cancer cells has been a promising approach that deserves attention in the field of anticancer drug development. To get a comprehensive understanding of the effect of the drug [Ru(η5-Cp)(PPh3)2CN] (RuCN) on cell lipidic components, we combine complementary analytical approaches, matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI TOF MS) and synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectroscopy. Techniques are used for screening the effect of potential metallodrug, RuCN, without and with drug carriers (carbon dots (CDs) and nitrogen-doped carbon dots (N-CDs)) on the lipids of the human ovarian cancer cell line A2780. MALDI TOF MS results revealed that the lysis of ovarian cancer membrane lipids is promoted by RuCN and not by drug carriers (CDs and N-CDs). Furthermore, SR-FTIR results strongly suggested that the phospholipids of cancer cells undergo oxidative stress after the treatment with RuCN that was accompanied by the disordering of the fatty acid chains. On the other hand, using (N-)CDs as RuCN nanocarriers prevented the oxidative stress caused by RuCN but did not prevent the disordering of the fatty acid chain packing. Finally, we demonstrated that RuCN and RuCN/(N-)CDs alter the hydration of the membrane surface in the membrane–water interface region.
T2  - Cancers
T1  - Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study
VL  - 14
IS  - 5
SP  - 1182
DO  - 10.3390/cancers14051182
ER  - 

@article{
author = "Nešić, Maja D. and Dučić, Tanja and Algarra, Manuel and Popović, Iva A. and Stepić, Milutin and Gonçalves, Mara and Petković, Marijana",
year = "2022",
abstract = "In the last decade, targeting membrane lipids in cancer cells has been a promising approach that deserves attention in the field of anticancer drug development. To get a comprehensive understanding of the effect of the drug [Ru(η5-Cp)(PPh3)2CN] (RuCN) on cell lipidic components, we combine complementary analytical approaches, matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI TOF MS) and synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectroscopy. Techniques are used for screening the effect of potential metallodrug, RuCN, without and with drug carriers (carbon dots (CDs) and nitrogen-doped carbon dots (N-CDs)) on the lipids of the human ovarian cancer cell line A2780. MALDI TOF MS results revealed that the lysis of ovarian cancer membrane lipids is promoted by RuCN and not by drug carriers (CDs and N-CDs). Furthermore, SR-FTIR results strongly suggested that the phospholipids of cancer cells undergo oxidative stress after the treatment with RuCN that was accompanied by the disordering of the fatty acid chains. On the other hand, using (N-)CDs as RuCN nanocarriers prevented the oxidative stress caused by RuCN but did not prevent the disordering of the fatty acid chain packing. Finally, we demonstrated that RuCN and RuCN/(N-)CDs alter the hydration of the membrane surface in the membrane–water interface region.",
journal = "Cancers",
title = "Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study",
volume = "14",
number = "5",
pages = "1182",
doi = "10.3390/cancers14051182"
}

Nešić, M. D., Dučić, T., Algarra, M., Popović, I. A., Stepić, M., Gonçalves, M.,& Petković, M.. (2022). Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study. in Cancers, 14(5), 1182.
https://doi.org/10.3390/cancers14051182

Nešić MD, Dučić T, Algarra M, Popović IA, Stepić M, Gonçalves M, Petković M. Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study. in Cancers. 2022;14(5):1182.
doi:10.3390/cancers14051182 .

Nešić, Maja D., Dučić, Tanja, Algarra, Manuel, Popović, Iva A., Stepić, Milutin, Gonçalves, Mara, Petković, Marijana, "Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study" in Cancers, 14, no. 5 (2022):1182,
https://doi.org/10.3390/cancers14051182 . .

TY  - JOUR
AU  - Dučić, Tanja
AU  - Alves, Carla S.
AU  - Vučinić, Željko
AU  - Lázaro-Martínez, Juan M.
AU  - Petković, Marijana
AU  - Soto, Juan
AU  - Mutavdžić, Dragosav
AU  - Valle Martínez de Yuso, M.
AU  - Radotić, Ksenija
AU  - Algarra, Manuel
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10277
AB  - S and N-doped carbon dots (S-CDs and N-CDs) and their cisplatin (cis-Pt) derivatives. (S-CDs@cis-Pt and N-CDs@cis-Pt) were tested on two ovarian cancer cell lines: A2780 and A2780 cells resistant to cis-Pt (A2780R). Several spectroscopic techniques were employed to check S-CDs@cis-Pt and N-CDs@cis-Pt: solid- and solution-state nuclear magnetic resonance, matrix-assisted laser desorption, ionization time-of-flight mass spectrometry, and X-ray photoelectron spectroscopy. In addition, synchrotron-based Fourier Transformed Infrared spectro-microscopy was used to evaluate the biochemical changes in cells after treatment with cis-Pt, S-CDs, N-CDs, or S-CDs@cis-Pt and N-CDs@cis-Pt, respectively. Computational chemistry was applied to establish the model for the most stable bond between S-CDs and N-CDs and cis-Pt. The results revealed the successful modification of S-CDs and N-CDs with cis-Pt and the formation of a stable composite system that can be used for drug delivery to cancer cells and likewise to overcome acquired cis-Pt resistance. Nanoparticle treatment of A2780 and A2780R cells led to the changes in their structure of lipids, proteins, and nucleic acids depending on the treatment. The results showed the S-CDs@cis-Pt and N-CDs@cis-Pt might be used in the combination with cis-Pt to treat the adenocarcinoma, thus having a potential to be further developed as drug delivery systems.
T2  - Journal of Colloid and Interface Science
T1  - S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach
VL  - 623
SP  - 226
EP  - 237
DO  - 10.1016/j.jcis.2022.05.005
ER  - 

@article{
author = "Dučić, Tanja and Alves, Carla S. and Vučinić, Željko and Lázaro-Martínez, Juan M. and Petković, Marijana and Soto, Juan and Mutavdžić, Dragosav and Valle Martínez de Yuso, M. and Radotić, Ksenija and Algarra, Manuel",
year = "2022",
abstract = "S and N-doped carbon dots (S-CDs and N-CDs) and their cisplatin (cis-Pt) derivatives. (S-CDs@cis-Pt and N-CDs@cis-Pt) were tested on two ovarian cancer cell lines: A2780 and A2780 cells resistant to cis-Pt (A2780R). Several spectroscopic techniques were employed to check S-CDs@cis-Pt and N-CDs@cis-Pt: solid- and solution-state nuclear magnetic resonance, matrix-assisted laser desorption, ionization time-of-flight mass spectrometry, and X-ray photoelectron spectroscopy. In addition, synchrotron-based Fourier Transformed Infrared spectro-microscopy was used to evaluate the biochemical changes in cells after treatment with cis-Pt, S-CDs, N-CDs, or S-CDs@cis-Pt and N-CDs@cis-Pt, respectively. Computational chemistry was applied to establish the model for the most stable bond between S-CDs and N-CDs and cis-Pt. The results revealed the successful modification of S-CDs and N-CDs with cis-Pt and the formation of a stable composite system that can be used for drug delivery to cancer cells and likewise to overcome acquired cis-Pt resistance. Nanoparticle treatment of A2780 and A2780R cells led to the changes in their structure of lipids, proteins, and nucleic acids depending on the treatment. The results showed the S-CDs@cis-Pt and N-CDs@cis-Pt might be used in the combination with cis-Pt to treat the adenocarcinoma, thus having a potential to be further developed as drug delivery systems.",
journal = "Journal of Colloid and Interface Science",
title = "S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach",
volume = "623",
pages = "226-237",
doi = "10.1016/j.jcis.2022.05.005"
}

Dučić, T., Alves, C. S., Vučinić, Ž., Lázaro-Martínez, J. M., Petković, M., Soto, J., Mutavdžić, D., Valle Martínez de Yuso, M., Radotić, K.,& Algarra, M.. (2022). S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach. in Journal of Colloid and Interface Science, 623, 226-237.
https://doi.org/10.1016/j.jcis.2022.05.005

Dučić T, Alves CS, Vučinić Ž, Lázaro-Martínez JM, Petković M, Soto J, Mutavdžić D, Valle Martínez de Yuso M, Radotić K, Algarra M. S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach. in Journal of Colloid and Interface Science. 2022;623:226-237.
doi:10.1016/j.jcis.2022.05.005 .

Dučić, Tanja, Alves, Carla S., Vučinić, Željko, Lázaro-Martínez, Juan M., Petković, Marijana, Soto, Juan, Mutavdžić, Dragosav, Valle Martínez de Yuso, M., Radotić, Ksenija, Algarra, Manuel, "S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach" in Journal of Colloid and Interface Science, 623 (2022):226-237,
https://doi.org/10.1016/j.jcis.2022.05.005 . .

TY  - JOUR
AU  - Nešić, Maja D.
AU  - Algarra, Manuel
AU  - Soto, Juan
AU  - Nenadović, Miloš
AU  - Popović, Iva A.
AU  - Petković, Marijana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9930
AB  - Metal acetate complexes are highly interesting in designing and synthesizing metal–organic frameworks, which have potential applications in gas storage, molecular sieves, ion exchange, and catalysis. Cadmium is well recognized as an industrial pollutant and a food contaminant that severely affects human health. For these reasons, the detection and analysis of Cd are of great interest. A major challenge in studying cadmium compounds' structure is that Cd2+ can have a wide range of coordination numbers and diverse geometries. (MA)LDI is extremely useful for detecting Cd(OAc)2·2H2O and investigating Cd's complex and flexible nature. We applied LDI to detect and characterize the Cd-related clusters and MALDI approach to investigate the Cd(OAc)2x2H2O reactivity and detectability with the five different organic matrices. The results revealed that in the solid-state Cd(OAc)2x2H2O tend to form coordination polymers, and in the presence of coordinating ligands (matrix molecules) form smaller complexes. In addition, the correlation between Cd-related LOD and matrix features was observed and discussed, indicating that the LODs of this analyte strongly depend on the optical absorption of the matrix-analyte adduct.
T2  - Polyhedron
T1  - Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts
VL  - 209
SP  - 115463
DO  - 10.1016/j.poly.2021.115463
ER  - 

@article{
author = "Nešić, Maja D. and Algarra, Manuel and Soto, Juan and Nenadović, Miloš and Popović, Iva A. and Petković, Marijana",
year = "2021",
abstract = "Metal acetate complexes are highly interesting in designing and synthesizing metal–organic frameworks, which have potential applications in gas storage, molecular sieves, ion exchange, and catalysis. Cadmium is well recognized as an industrial pollutant and a food contaminant that severely affects human health. For these reasons, the detection and analysis of Cd are of great interest. A major challenge in studying cadmium compounds' structure is that Cd2+ can have a wide range of coordination numbers and diverse geometries. (MA)LDI is extremely useful for detecting Cd(OAc)2·2H2O and investigating Cd's complex and flexible nature. We applied LDI to detect and characterize the Cd-related clusters and MALDI approach to investigate the Cd(OAc)2x2H2O reactivity and detectability with the five different organic matrices. The results revealed that in the solid-state Cd(OAc)2x2H2O tend to form coordination polymers, and in the presence of coordinating ligands (matrix molecules) form smaller complexes. In addition, the correlation between Cd-related LOD and matrix features was observed and discussed, indicating that the LODs of this analyte strongly depend on the optical absorption of the matrix-analyte adduct.",
journal = "Polyhedron",
title = "Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts",
volume = "209",
pages = "115463",
doi = "10.1016/j.poly.2021.115463"
}

Nešić, M. D., Algarra, M., Soto, J., Nenadović, M., Popović, I. A.,& Petković, M.. (2021). Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts. in Polyhedron, 209, 115463.
https://doi.org/10.1016/j.poly.2021.115463

Nešić MD, Algarra M, Soto J, Nenadović M, Popović IA, Petković M. Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts. in Polyhedron. 2021;209:115463.
doi:10.1016/j.poly.2021.115463 .

Nešić, Maja D., Algarra, Manuel, Soto, Juan, Nenadović, Miloš, Popović, Iva A., Petković, Marijana, "Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts" in Polyhedron, 209 (2021):115463,
https://doi.org/10.1016/j.poly.2021.115463 . .

TY  - JOUR
AU  - Houdová, Dominika
AU  - Soto, Juan
AU  - Castro, Rita
AU  - Rodrigues, João
AU  - Soledad Pino-González, Ma
AU  - Petković, Marijana
AU  - Bandosz, Teresa J
AU  - Algarra, Manuel
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9127
AB  - A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time-of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+, cholesterol and DHB, and it was supported by theoretical calculations. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+. © 2021 Elsevier Inc.
T2  - Journal of Colloid and Interface Science
T1  - Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry
VL  - 591
SP  - 373
EP  - 383
DO  - 10.1016/j.jcis.2021.02.004
ER  - 

@article{
author = "Houdová, Dominika and Soto, Juan and Castro, Rita and Rodrigues, João and Soledad Pino-González, Ma and Petković, Marijana and Bandosz, Teresa J and Algarra, Manuel",
year = "2021",
abstract = "A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time-of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+, cholesterol and DHB, and it was supported by theoretical calculations. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+. © 2021 Elsevier Inc.",
journal = "Journal of Colloid and Interface Science",
title = "Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry",
volume = "591",
pages = "373-383",
doi = "10.1016/j.jcis.2021.02.004"
}

Houdová, D., Soto, J., Castro, R., Rodrigues, J., Soledad Pino-González, M., Petković, M., Bandosz, T. J.,& Algarra, M.. (2021). Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. in Journal of Colloid and Interface Science, 591, 373-383.
https://doi.org/10.1016/j.jcis.2021.02.004

Houdová D, Soto J, Castro R, Rodrigues J, Soledad Pino-González M, Petković M, Bandosz TJ, Algarra M. Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. in Journal of Colloid and Interface Science. 2021;591:373-383.
doi:10.1016/j.jcis.2021.02.004 .

Houdová, Dominika, Soto, Juan, Castro, Rita, Rodrigues, João, Soledad Pino-González, Ma, Petković, Marijana, Bandosz, Teresa J, Algarra, Manuel, "Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry" in Journal of Colloid and Interface Science, 591 (2021):373-383,
https://doi.org/10.1016/j.jcis.2021.02.004 . .

TY  - JOUR
AU  - Houdová, Dominika
AU  - Soto, Juan
AU  - Castro, Rita
AU  - Rodrigues, João
AU  - Soledad Pino-González, Ma
AU  - Petković, Marijana
AU  - Bandosz, Teresa J
AU  - Algarra, Manuel
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9145
AB  - A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time-of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+, cholesterol and DHB, and it was supported by theoretical calculations. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+. © 2021 Elsevier Inc.
T2  - Journal of Colloid and Interface Science
T1  - Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry
VL  - 591
SP  - 373
EP  - 383
DO  - 10.1016/j.jcis.2021.02.004
ER  - 

@article{
author = "Houdová, Dominika and Soto, Juan and Castro, Rita and Rodrigues, João and Soledad Pino-González, Ma and Petković, Marijana and Bandosz, Teresa J and Algarra, Manuel",
year = "2021",
abstract = "A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time-of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+, cholesterol and DHB, and it was supported by theoretical calculations. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+. © 2021 Elsevier Inc.",
journal = "Journal of Colloid and Interface Science",
title = "Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry",
volume = "591",
pages = "373-383",
doi = "10.1016/j.jcis.2021.02.004"
}

Houdová, D., Soto, J., Castro, R., Rodrigues, J., Soledad Pino-González, M., Petković, M., Bandosz, T. J.,& Algarra, M.. (2021). Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. in Journal of Colloid and Interface Science, 591, 373-383.
https://doi.org/10.1016/j.jcis.2021.02.004

Houdová D, Soto J, Castro R, Rodrigues J, Soledad Pino-González M, Petković M, Bandosz TJ, Algarra M. Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. in Journal of Colloid and Interface Science. 2021;591:373-383.
doi:10.1016/j.jcis.2021.02.004 .

Houdová, Dominika, Soto, Juan, Castro, Rita, Rodrigues, João, Soledad Pino-González, Ma, Petković, Marijana, Bandosz, Teresa J, Algarra, Manuel, "Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry" in Journal of Colloid and Interface Science, 591 (2021):373-383,
https://doi.org/10.1016/j.jcis.2021.02.004 . .

TY  - JOUR
AU  - Nešić, Maja D.
AU  - Dučić, Tanja
AU  - Liang, Xinyue
AU  - Algarra, Manuel
AU  - Mi, Lan
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Kop, Tatjana
AU  - Bjelaković, Mira
AU  - Mitrović, Aleksandra
AU  - Gojgić Cvijović, Gordana D.
AU  - Stepić, Milutin
AU  - Petković, Marijana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9712
AB  - Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide I band, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60 potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells.
T2  - International Journal of Biological Macromolecules
T1  - SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives
VL  - 165
SP  - 2541
EP  - 2549
DO  - 10.1016/j.ijbiomac.2020.10.141
ER  - 

@article{
author = "Nešić, Maja D. and Dučić, Tanja and Liang, Xinyue and Algarra, Manuel and Mi, Lan and Korićanac, Lela and Žakula, Jelena and Kop, Tatjana and Bjelaković, Mira and Mitrović, Aleksandra and Gojgić Cvijović, Gordana D. and Stepić, Milutin and Petković, Marijana",
year = "2020",
abstract = "Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide I band, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60 potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells.",
journal = "International Journal of Biological Macromolecules",
title = "SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives",
volume = "165",
pages = "2541-2549",
doi = "10.1016/j.ijbiomac.2020.10.141"
}

Nešić, M. D., Dučić, T., Liang, X., Algarra, M., Mi, L., Korićanac, L., Žakula, J., Kop, T., Bjelaković, M., Mitrović, A., Gojgić Cvijović, G. D., Stepić, M.,& Petković, M.. (2020). SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives. in International Journal of Biological Macromolecules, 165, 2541-2549.
https://doi.org/10.1016/j.ijbiomac.2020.10.141

Nešić MD, Dučić T, Liang X, Algarra M, Mi L, Korićanac L, Žakula J, Kop T, Bjelaković M, Mitrović A, Gojgić Cvijović GD, Stepić M, Petković M. SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives. in International Journal of Biological Macromolecules. 2020;165:2541-2549.
doi:10.1016/j.ijbiomac.2020.10.141 .

Nešić, Maja D., Dučić, Tanja, Liang, Xinyue, Algarra, Manuel, Mi, Lan, Korićanac, Lela, Žakula, Jelena, Kop, Tatjana, Bjelaković, Mira, Mitrović, Aleksandra, Gojgić Cvijović, Gordana D., Stepić, Milutin, Petković, Marijana, "SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives" in International Journal of Biological Macromolecules, 165 (2020):2541-2549,
https://doi.org/10.1016/j.ijbiomac.2020.10.141 . .