TY  - CONF
AU  - Đorđević, Neda O.
AU  - Keta, Otilija D.
AU  - Petković, Vladana
AU  - Todorović Vukotić, Nevena
AU  - Stanković, Dalibor
AU  - Tešević, V.
AU  - Pajović, Snežana B.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12594
AB  - This study aimed to determine the phenolic compound content, in vitro antioxidative potential, and cytotoxic effects of four red wine samples: a commercial (V) and three clonal wines (V1, V2, and V3). LC/MS-MS, cyclic voltammetry, and MTT assay techniques were employed for this purpose. Results revealed that all wines were rich in phenolic compounds. Clonal wines outperformed the commercial ones in most phenolic compounds (except myricetin). Notably, V2 and V3 showed the highest levels of gallic acid, catechin, and epicatechin. Among them, V3 exhibited superior antioxidative activity. The MTT assay demonstrated stronger cytotoxic effects of the wine samples on pancreas (Bx-PC3) and colon (HT29) carcinoma cells (47% to 16% and 27% to 7% compared to control, respectively) than on the normal lung fibroblasts (MRC-5) cell line (106% to 77%). It can be concluded that HT29 cells were more sensitive than Bx-PC3 cells. Finally, both clonal and commercial wines serve as valuable sources of polyphenolic compounds, which could have a significant role in preventing cancer and diseases related to oxidative stress.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - In vitro biological effects of clonal red wines
SP  - 180
EP  - 183
DO  - 10.46793/ICCBI23.180DJ
ER  - 

@conference{
author = "Đorđević, Neda O. and Keta, Otilija D. and Petković, Vladana and Todorović Vukotić, Nevena and Stanković, Dalibor and Tešević, V. and Pajović, Snežana B.",
year = "2023",
abstract = "This study aimed to determine the phenolic compound content, in vitro antioxidative potential, and cytotoxic effects of four red wine samples: a commercial (V) and three clonal wines (V1, V2, and V3). LC/MS-MS, cyclic voltammetry, and MTT assay techniques were employed for this purpose. Results revealed that all wines were rich in phenolic compounds. Clonal wines outperformed the commercial ones in most phenolic compounds (except myricetin). Notably, V2 and V3 showed the highest levels of gallic acid, catechin, and epicatechin. Among them, V3 exhibited superior antioxidative activity. The MTT assay demonstrated stronger cytotoxic effects of the wine samples on pancreas (Bx-PC3) and colon (HT29) carcinoma cells (47% to 16% and 27% to 7% compared to control, respectively) than on the normal lung fibroblasts (MRC-5) cell line (106% to 77%). It can be concluded that HT29 cells were more sensitive than Bx-PC3 cells. Finally, both clonal and commercial wines serve as valuable sources of polyphenolic compounds, which could have a significant role in preventing cancer and diseases related to oxidative stress.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "In vitro biological effects of clonal red wines",
pages = "180-183",
doi = "10.46793/ICCBI23.180DJ"
}

Đorđević, N. O., Keta, O. D., Petković, V., Todorović Vukotić, N., Stanković, D., Tešević, V.,& Pajović, S. B.. (2023). In vitro biological effects of clonal red wines. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 180-183.
https://doi.org/10.46793/ICCBI23.180DJ

Đorđević NO, Keta OD, Petković V, Todorović Vukotić N, Stanković D, Tešević V, Pajović SB. In vitro biological effects of clonal red wines. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:180-183.
doi:10.46793/ICCBI23.180DJ .

Đorđević, Neda O., Keta, Otilija D., Petković, Vladana, Todorović Vukotić, Nevena, Stanković, Dalibor, Tešević, V., Pajović, Snežana B., "In vitro biological effects of clonal red wines" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):180-183,
https://doi.org/10.46793/ICCBI23.180DJ . .

TY  - CONF
AU  - Keta, Otilija D.
AU  - Petković, Vladana
AU  - Đorđević, Neda O.
AU  - Đorđević, Miloš
AU  - Ivković, Branka
AU  - Dobričić, Vladimir
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12088
AB  - Given the well-established potential of chalcones in modulating the response of cancer cells to therapeutic interventions, coupled with the growing imperative to enhance their biological attributes, the objective of this study was to synthesize a methoxy-substituted chalcone (OCH3) and assess its capacity to amplify the inhibitory effects of radiation in melanoma cells known for their resistance to radiotherapy. The A375 melanoma cells were subjected to a clinically relevant dose of 2 Gy gamma irradiation. OCH3 was employed either as a standalone treatment or in conjunction with irradiation. The obtained results unveiled the substantial radiosensitizing potential of OCH3 within this specific cell line. Our subsequent investigations will be designed to investigate the underlying mechanisms that contribute to the radiosensitizing properties of OCH3. Moreover, we intend to evaluate the efficacy of OCH3 against other types of radioresistant cancer cells. The presented data not only illuminates the enhanced therapeutic possibilities offered by OCH3 but also highlights its potential as a valuable agent in addressing a wider array of challenging malignancies.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - The effects of a selected methoxy substituted chalcone in human melanoma cells irradiated with γ-rays
SP  - 471
EP  - 474
DO  - 10.46793/ICCBI23.471K
ER  - 

@conference{
author = "Keta, Otilija D. and Petković, Vladana and Đorđević, Neda O. and Đorđević, Miloš and Ivković, Branka and Dobričić, Vladimir",
year = "2023",
abstract = "Given the well-established potential of chalcones in modulating the response of cancer cells to therapeutic interventions, coupled with the growing imperative to enhance their biological attributes, the objective of this study was to synthesize a methoxy-substituted chalcone (OCH3) and assess its capacity to amplify the inhibitory effects of radiation in melanoma cells known for their resistance to radiotherapy. The A375 melanoma cells were subjected to a clinically relevant dose of 2 Gy gamma irradiation. OCH3 was employed either as a standalone treatment or in conjunction with irradiation. The obtained results unveiled the substantial radiosensitizing potential of OCH3 within this specific cell line. Our subsequent investigations will be designed to investigate the underlying mechanisms that contribute to the radiosensitizing properties of OCH3. Moreover, we intend to evaluate the efficacy of OCH3 against other types of radioresistant cancer cells. The presented data not only illuminates the enhanced therapeutic possibilities offered by OCH3 but also highlights its potential as a valuable agent in addressing a wider array of challenging malignancies.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "The effects of a selected methoxy substituted chalcone in human melanoma cells irradiated with γ-rays",
pages = "471-474",
doi = "10.46793/ICCBI23.471K"
}

Keta, O. D., Petković, V., Đorđević, N. O., Đorđević, M., Ivković, B.,& Dobričić, V.. (2023). The effects of a selected methoxy substituted chalcone in human melanoma cells irradiated with γ-rays. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 471-474.
https://doi.org/10.46793/ICCBI23.471K

Keta OD, Petković V, Đorđević NO, Đorđević M, Ivković B, Dobričić V. The effects of a selected methoxy substituted chalcone in human melanoma cells irradiated with γ-rays. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:471-474.
doi:10.46793/ICCBI23.471K .

Keta, Otilija D., Petković, Vladana, Đorđević, Neda O., Đorđević, Miloš, Ivković, Branka, Dobričić, Vladimir, "The effects of a selected methoxy substituted chalcone in human melanoma cells irradiated with γ-rays" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):471-474,
https://doi.org/10.46793/ICCBI23.471K . .

TY  - CONF
AU  - Keta, Otilija D.
AU  - Bošković, Jelena
AU  - Petković, Vladana
AU  - Đorđević, Neda O.
AU  - Dobričić, Vladimir
AU  - Čudina, Olivera
AU  - Pajović, Snežana B.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12089
AB  - Among novel cancer chemotherapy approaches, the use of cyclooxygenases (COXs) and lipoxygenases (LOXs) inhibitors represents a promising mean for cancer treatment showing lesser toxicity comparing to the currently used cytotoxic drugs. This study detailed the synthesis of three novel compounds: 1ME, BHTK-AA, and IBU-Ac, each with the capability to concurrently inhibit both COX-2 and 5-LOX. Subsequently, we assessed their effectiveness in inhibiting the proliferation of HeLa cervical and MIA PaCa-2 pancreatic cancer cells. The IC50 values for both examined cell lines were approximately 40 μM, indicating the promising inhibitory potential of the IBU-Ac compound in both types of cancer cells. This finding is positioned to stimulate further investigation into the potential application of IBU-Ac against these particular types of cancers, while also advocating its use in combination with standard anti-cancer protocols, i.e., chemoterapeutics or radiation therapy. The results of this work are also advocating the development and refinement of dual COX-2 and 5-LOX inhibitors, thus improving their efficacy and safety.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - Synthesis and cytotoxic activity of selected dual COX-2 and 5-LOX inhibitors in HeLa and MIA PaCa-2 human cancer cell lines
SP  - 503
EP  - 506
DO  - 10.46793/ICCBI23.503K
ER  - 

@conference{
author = "Keta, Otilija D. and Bošković, Jelena and Petković, Vladana and Đorđević, Neda O. and Dobričić, Vladimir and Čudina, Olivera and Pajović, Snežana B.",
year = "2023",
abstract = "Among novel cancer chemotherapy approaches, the use of cyclooxygenases (COXs) and lipoxygenases (LOXs) inhibitors represents a promising mean for cancer treatment showing lesser toxicity comparing to the currently used cytotoxic drugs. This study detailed the synthesis of three novel compounds: 1ME, BHTK-AA, and IBU-Ac, each with the capability to concurrently inhibit both COX-2 and 5-LOX. Subsequently, we assessed their effectiveness in inhibiting the proliferation of HeLa cervical and MIA PaCa-2 pancreatic cancer cells. The IC50 values for both examined cell lines were approximately 40 μM, indicating the promising inhibitory potential of the IBU-Ac compound in both types of cancer cells. This finding is positioned to stimulate further investigation into the potential application of IBU-Ac against these particular types of cancers, while also advocating its use in combination with standard anti-cancer protocols, i.e., chemoterapeutics or radiation therapy. The results of this work are also advocating the development and refinement of dual COX-2 and 5-LOX inhibitors, thus improving their efficacy and safety.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "Synthesis and cytotoxic activity of selected dual COX-2 and 5-LOX inhibitors in HeLa and MIA PaCa-2 human cancer cell lines",
pages = "503-506",
doi = "10.46793/ICCBI23.503K"
}

Keta, O. D., Bošković, J., Petković, V., Đorđević, N. O., Dobričić, V., Čudina, O.,& Pajović, S. B.. (2023). Synthesis and cytotoxic activity of selected dual COX-2 and 5-LOX inhibitors in HeLa and MIA PaCa-2 human cancer cell lines. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 503-506.
https://doi.org/10.46793/ICCBI23.503K

Keta OD, Bošković J, Petković V, Đorđević NO, Dobričić V, Čudina O, Pajović SB. Synthesis and cytotoxic activity of selected dual COX-2 and 5-LOX inhibitors in HeLa and MIA PaCa-2 human cancer cell lines. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:503-506.
doi:10.46793/ICCBI23.503K .

Keta, Otilija D., Bošković, Jelena, Petković, Vladana, Đorđević, Neda O., Dobričić, Vladimir, Čudina, Olivera, Pajović, Snežana B., "Synthesis and cytotoxic activity of selected dual COX-2 and 5-LOX inhibitors in HeLa and MIA PaCa-2 human cancer cell lines" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):503-506,
https://doi.org/10.46793/ICCBI23.503K . .

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Petković, Vladana
AU  - Cirrone, Pablo
AU  - Petringa, Giada
AU  - Cuttone, Giacomo
AU  - Sakata, Dousatsu
AU  - Shin, Wook-Geun
AU  - Incerti, Sebastien
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9875
AB  - Purpose The complex relationship between linear energy transfer (LET) and cellular response to radiation is not yet fully elucidated. To better characterize DNA damage after irradiations with therapeutic protons, we monitored formation and disappearance of DNA double-strand breaks (DNA DSB) as a function of LET and time. Comparisons with conventional γ-rays and high LET carbon ions were also performed.Materials and Methods In the present work, we performed immunofluorescence-based assay to determine the amount of DNA DSB induced by different LET values along the 62 MeV therapeutic proton Spread out Bragg peak (SOBP) in three cancer cell lines, i.e. HTB140 melanoma, MCF-7 breast adenocarcinoma and HTB177 non-small lung cancer cells. Time dependence of foci formation was followed as well. To determine irradiation positions, corresponding to the desired LET values, numerical simulations were carried out using Geant4 toolkit. We compared γ-H2AX foci persistence after irradiations with protons to that of γ-rays and carbon ions.Results With the rise of LET values along the therapeutic proton SOBP, the increase of γ-H2AX foci number is detected in the three cell lines up to the distal end of the SOBP, while there is a decrease on its distal fall-off part. With the prolonged incubation time, the number of foci gradually drops tending to attain the residual level. For the maximum number of DNA DSB, irradiation with protons attain higher level than that of γ-rays. Carbon ions produce more DNA DSB than protons but not substantially. The number of residual foci produced by γ-rays is significantly lower than that of protons and particularly carbon ions. Carbon ions do not produce considerably higher number of foci than protons, as it could be expected due to their physical properties.Conclusions In situ visualization of γ-H2AX foci reveal creation of more lesions in the three cell lines by clinically relevant proton SOBP than γ-rays. The lack of significant differences in the number of γ-H2AX foci between the proton and carbon ion-irradiated samples suggests an increased complexity of DNA lesions and slower repair kinetics after carbon ions compared to protons. For all three irradiation types, there is no major difference between the three cell lines shortly after irradiations, while later on, the formation of residual foci starts to express the inherent nature of tested cells, therefore increasing discrepancy between them.
T2  - International Journal of Radiation Biology
T1  - DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time
VL  - 97
IS  - 9
SP  - 1229
EP  - 1240
DO  - 10.1080/09553002.2021.1948140
ER  - 

@article{
author = "Keta, Otilija D. and Petković, Vladana and Cirrone, Pablo and Petringa, Giada and Cuttone, Giacomo and Sakata, Dousatsu and Shin, Wook-Geun and Incerti, Sebastien and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2021",
abstract = "Purpose The complex relationship between linear energy transfer (LET) and cellular response to radiation is not yet fully elucidated. To better characterize DNA damage after irradiations with therapeutic protons, we monitored formation and disappearance of DNA double-strand breaks (DNA DSB) as a function of LET and time. Comparisons with conventional γ-rays and high LET carbon ions were also performed.Materials and Methods In the present work, we performed immunofluorescence-based assay to determine the amount of DNA DSB induced by different LET values along the 62 MeV therapeutic proton Spread out Bragg peak (SOBP) in three cancer cell lines, i.e. HTB140 melanoma, MCF-7 breast adenocarcinoma and HTB177 non-small lung cancer cells. Time dependence of foci formation was followed as well. To determine irradiation positions, corresponding to the desired LET values, numerical simulations were carried out using Geant4 toolkit. We compared γ-H2AX foci persistence after irradiations with protons to that of γ-rays and carbon ions.Results With the rise of LET values along the therapeutic proton SOBP, the increase of γ-H2AX foci number is detected in the three cell lines up to the distal end of the SOBP, while there is a decrease on its distal fall-off part. With the prolonged incubation time, the number of foci gradually drops tending to attain the residual level. For the maximum number of DNA DSB, irradiation with protons attain higher level than that of γ-rays. Carbon ions produce more DNA DSB than protons but not substantially. The number of residual foci produced by γ-rays is significantly lower than that of protons and particularly carbon ions. Carbon ions do not produce considerably higher number of foci than protons, as it could be expected due to their physical properties.Conclusions In situ visualization of γ-H2AX foci reveal creation of more lesions in the three cell lines by clinically relevant proton SOBP than γ-rays. The lack of significant differences in the number of γ-H2AX foci between the proton and carbon ion-irradiated samples suggests an increased complexity of DNA lesions and slower repair kinetics after carbon ions compared to protons. For all three irradiation types, there is no major difference between the three cell lines shortly after irradiations, while later on, the formation of residual foci starts to express the inherent nature of tested cells, therefore increasing discrepancy between them.",
journal = "International Journal of Radiation Biology",
title = "DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time",
volume = "97",
number = "9",
pages = "1229-1240",
doi = "10.1080/09553002.2021.1948140"
}

Keta, O. D., Petković, V., Cirrone, P., Petringa, G., Cuttone, G., Sakata, D., Shin, W., Incerti, S., Petrović, I. M.,& Ristić-Fira, A.. (2021). DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time. in International Journal of Radiation Biology, 97(9), 1229-1240.
https://doi.org/10.1080/09553002.2021.1948140

Keta OD, Petković V, Cirrone P, Petringa G, Cuttone G, Sakata D, Shin W, Incerti S, Petrović IM, Ristić-Fira A. DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time. in International Journal of Radiation Biology. 2021;97(9):1229-1240.
doi:10.1080/09553002.2021.1948140 .

Keta, Otilija D., Petković, Vladana, Cirrone, Pablo, Petringa, Giada, Cuttone, Giacomo, Sakata, Dousatsu, Shin, Wook-Geun, Incerti, Sebastien, Petrović, Ivan M., Ristić-Fira, Aleksandra, "DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time" in International Journal of Radiation Biology, 97, no. 9 (2021):1229-1240,
https://doi.org/10.1080/09553002.2021.1948140 . .

TY  - JOUR
AU  - Petringa, Giada
AU  - Calvaruso, Marco
AU  - Conte, Valeria
AU  - Bláha, Pavel
AU  - Bravatà, Valentina
AU  - Cammarata, Francesco Paolo
AU  - Cuttone, Giacomo
AU  - Forte, Giusi Irma
AU  - Keta, Otilija D.
AU  - Manti, Lorenzo
AU  - Minafra, Luigi
AU  - Petković, Vladana
AU  - Petrović, Ivan M.
AU  - Richiusa, Selene
AU  - Ristić-Fira, Aleksandra
AU  - Russo, Giorgio
AU  - Cirrone, Giuseppe Antonio Pablo
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9958
AB  - CATANA (Centro di AdroTerapia ed Applicazioni Nucleari Avanzate) was the first Italian protontherapy facility dedicated to the treatment of ocular neoplastic pathologies. It is in operation at the LNS Laboratories of the Italian Institute for Nuclear Physics (INFN-LNS) and to date, 500 patients have been successfully treated. Even though proton therapy has demonstrated success in clinical settings, there is still a need for more accurate models because they are crucial for the estimation of clinically relevant RBE values. Since RBE can vary depending on several physical and biological parameters, there is a clear need for more experimental data to generate predictions. Establishing a database of cell survival experiments is therefore useful to accurately predict the effects of irradiations on both cancerous and normal tissue. The main aim of this work was to compare RBE values obtained from in-vitro experimental data with predictions made by the LEM II (Local Effect Model), Monte Carlo approaches, and semi-empirical models based on LET experimental measurements. For this purpose, the 92.1 uveal melanoma and ARPE-19 cells derived from normal retinal pigmented epithelium were selected and irradiated in the middle of clinical SOBP of the CATANA proton therapy facility. The remarkable results show the potentiality of using microdosimetric spectrum, Monte Carlo simulations and LEM model to predict not only the RBE but also the survival curves.
T2  - Applied Sciences
T1  - Radiobiological Outcomes, Microdosimetric Evaluations and Monte Carlo Predictions in Eye Proton Therapy
VL  - 11
IS  - 19
SP  - 8822
DO  - 10.3390/app11198822
ER  - 

@article{
author = "Petringa, Giada and Calvaruso, Marco and Conte, Valeria and Bláha, Pavel and Bravatà, Valentina and Cammarata, Francesco Paolo and Cuttone, Giacomo and Forte, Giusi Irma and Keta, Otilija D. and Manti, Lorenzo and Minafra, Luigi and Petković, Vladana and Petrović, Ivan M. and Richiusa, Selene and Ristić-Fira, Aleksandra and Russo, Giorgio and Cirrone, Giuseppe Antonio Pablo",
year = "2021",
abstract = "CATANA (Centro di AdroTerapia ed Applicazioni Nucleari Avanzate) was the first Italian protontherapy facility dedicated to the treatment of ocular neoplastic pathologies. It is in operation at the LNS Laboratories of the Italian Institute for Nuclear Physics (INFN-LNS) and to date, 500 patients have been successfully treated. Even though proton therapy has demonstrated success in clinical settings, there is still a need for more accurate models because they are crucial for the estimation of clinically relevant RBE values. Since RBE can vary depending on several physical and biological parameters, there is a clear need for more experimental data to generate predictions. Establishing a database of cell survival experiments is therefore useful to accurately predict the effects of irradiations on both cancerous and normal tissue. The main aim of this work was to compare RBE values obtained from in-vitro experimental data with predictions made by the LEM II (Local Effect Model), Monte Carlo approaches, and semi-empirical models based on LET experimental measurements. For this purpose, the 92.1 uveal melanoma and ARPE-19 cells derived from normal retinal pigmented epithelium were selected and irradiated in the middle of clinical SOBP of the CATANA proton therapy facility. The remarkable results show the potentiality of using microdosimetric spectrum, Monte Carlo simulations and LEM model to predict not only the RBE but also the survival curves.",
journal = "Applied Sciences",
title = "Radiobiological Outcomes, Microdosimetric Evaluations and Monte Carlo Predictions in Eye Proton Therapy",
volume = "11",
number = "19",
pages = "8822",
doi = "10.3390/app11198822"
}

Petringa, G., Calvaruso, M., Conte, V., Bláha, P., Bravatà, V., Cammarata, F. P., Cuttone, G., Forte, G. I., Keta, O. D., Manti, L., Minafra, L., Petković, V., Petrović, I. M., Richiusa, S., Ristić-Fira, A., Russo, G.,& Cirrone, G. A. P.. (2021). Radiobiological Outcomes, Microdosimetric Evaluations and Monte Carlo Predictions in Eye Proton Therapy. in Applied Sciences, 11(19), 8822.
https://doi.org/10.3390/app11198822

Petringa G, Calvaruso M, Conte V, Bláha P, Bravatà V, Cammarata FP, Cuttone G, Forte GI, Keta OD, Manti L, Minafra L, Petković V, Petrović IM, Richiusa S, Ristić-Fira A, Russo G, Cirrone GAP. Radiobiological Outcomes, Microdosimetric Evaluations and Monte Carlo Predictions in Eye Proton Therapy. in Applied Sciences. 2021;11(19):8822.
doi:10.3390/app11198822 .

Petringa, Giada, Calvaruso, Marco, Conte, Valeria, Bláha, Pavel, Bravatà, Valentina, Cammarata, Francesco Paolo, Cuttone, Giacomo, Forte, Giusi Irma, Keta, Otilija D., Manti, Lorenzo, Minafra, Luigi, Petković, Vladana, Petrović, Ivan M., Richiusa, Selene, Ristić-Fira, Aleksandra, Russo, Giorgio, Cirrone, Giuseppe Antonio Pablo, "Radiobiological Outcomes, Microdosimetric Evaluations and Monte Carlo Predictions in Eye Proton Therapy" in Applied Sciences, 11, no. 19 (2021):8822,
https://doi.org/10.3390/app11198822 . .

TY  - JOUR
AU  - Petrović, Marija
AU  - Popović, Suzana
AU  - Baskić, Dejan
AU  - Todorović, Miloš
AU  - Đurđević, Predrag
AU  - Ristić-Fira, Aleksandra
AU  - Keta, Otilija D.
AU  - Petković, Vladana
AU  - Korićanac, Lela
AU  - Stojković, Danijela
AU  - Jevtić, Verica
AU  - Trifunović, Srećko
AU  - Todorović, Danijela
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9631
AB  - Aim: Newly synthesized platinum(IV) complexes with ethylenediamine-N,N’-diacetate ligands (EDDA-type) (butyl-Pt and pentyl-Pt) were investigated against two cancer (A549 lung, and HTB 140 melanoma) and one non-cancerous (MRC-5 embryonic lung fibroblast) human cell lines. Materials and Methods: The effects of these agents were compared with those of cisplatin after 6-, 24- and 48-h treatment. Sulforhodamine-B (SRB) assay was performed to estimate the cytotoxic effect, while the inhibitory effect on cell proliferation was measured using 5-bromo-2,-deoxyuridine (BrdU) incorporation assay. Cell cycle analysis was performed by flow cytometry. Type of cell death induced by these agents was determined by electrophoretic analysis of DNA, flow cytometry and by western blot analysis of proteins involved in induction of apoptosis. The effects of gamma irradiation, alone and in combination with platinum-based compounds, were examined by clonogenic and SRB assays. Results: All examined platinum-based compounds had inhibitory and antiproliferative effects on A549 cells, but not on HTB140 and MRC-5 cells. Butyl-Pt, pentyl-Pt and cisplatin arrested the cell cycle in the S-phase and induced apoptotic cell death via regulation of expression of B-cell lymphoma 2 (BCL2) and BCL2-associated X (BAX) proteins. Platinum-based compounds increased the sensitivity of A549 cells to gamma irradiation. Butyl-Pt and pentyl-Pt showed better antitumour effects against A549 cells than did cisplatin, by interfering in cell proliferation and the cell cycle, and by triggering apoptosis. Conclusion: The effects of gamma irradiation on tumour cells may be amplified by pre-treatment of cells with platinum-based compounds.
T2  - Anticancer Research
T1  - The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro
VL  - 40
IS  - 9
SP  - 5001
EP  - 5013
DO  - 10.21873/anticanres.14503
ER  - 

@article{
author = "Petrović, Marija and Popović, Suzana and Baskić, Dejan and Todorović, Miloš and Đurđević, Predrag and Ristić-Fira, Aleksandra and Keta, Otilija D. and Petković, Vladana and Korićanac, Lela and Stojković, Danijela and Jevtić, Verica and Trifunović, Srećko and Todorović, Danijela",
year = "2020",
abstract = "Aim: Newly synthesized platinum(IV) complexes with ethylenediamine-N,N’-diacetate ligands (EDDA-type) (butyl-Pt and pentyl-Pt) were investigated against two cancer (A549 lung, and HTB 140 melanoma) and one non-cancerous (MRC-5 embryonic lung fibroblast) human cell lines. Materials and Methods: The effects of these agents were compared with those of cisplatin after 6-, 24- and 48-h treatment. Sulforhodamine-B (SRB) assay was performed to estimate the cytotoxic effect, while the inhibitory effect on cell proliferation was measured using 5-bromo-2,-deoxyuridine (BrdU) incorporation assay. Cell cycle analysis was performed by flow cytometry. Type of cell death induced by these agents was determined by electrophoretic analysis of DNA, flow cytometry and by western blot analysis of proteins involved in induction of apoptosis. The effects of gamma irradiation, alone and in combination with platinum-based compounds, were examined by clonogenic and SRB assays. Results: All examined platinum-based compounds had inhibitory and antiproliferative effects on A549 cells, but not on HTB140 and MRC-5 cells. Butyl-Pt, pentyl-Pt and cisplatin arrested the cell cycle in the S-phase and induced apoptotic cell death via regulation of expression of B-cell lymphoma 2 (BCL2) and BCL2-associated X (BAX) proteins. Platinum-based compounds increased the sensitivity of A549 cells to gamma irradiation. Butyl-Pt and pentyl-Pt showed better antitumour effects against A549 cells than did cisplatin, by interfering in cell proliferation and the cell cycle, and by triggering apoptosis. Conclusion: The effects of gamma irradiation on tumour cells may be amplified by pre-treatment of cells with platinum-based compounds.",
journal = "Anticancer Research",
title = "The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro",
volume = "40",
number = "9",
pages = "5001-5013",
doi = "10.21873/anticanres.14503"
}

Petrović, M., Popović, S., Baskić, D., Todorović, M., Đurđević, P., Ristić-Fira, A., Keta, O. D., Petković, V., Korićanac, L., Stojković, D., Jevtić, V., Trifunović, S.,& Todorović, D.. (2020). The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro. in Anticancer Research, 40(9), 5001-5013.
https://doi.org/10.21873/anticanres.14503

Petrović M, Popović S, Baskić D, Todorović M, Đurđević P, Ristić-Fira A, Keta OD, Petković V, Korićanac L, Stojković D, Jevtić V, Trifunović S, Todorović D. The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro. in Anticancer Research. 2020;40(9):5001-5013.
doi:10.21873/anticanres.14503 .

Petrović, Marija, Popović, Suzana, Baskić, Dejan, Todorović, Miloš, Đurđević, Predrag, Ristić-Fira, Aleksandra, Keta, Otilija D., Petković, Vladana, Korićanac, Lela, Stojković, Danijela, Jevtić, Verica, Trifunović, Srećko, Todorović, Danijela, "The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro" in Anticancer Research, 40, no. 9 (2020):5001-5013,
https://doi.org/10.21873/anticanres.14503 . .

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Deljanin, Milena
AU  - Petković, Vladana
AU  - Zdunić, Gordana
AU  - Janković, Teodora
AU  - Živković, Jelena
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
AU  - Šavikin, Katarina
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8996
AB  - The aim of the present study was to investigate effects of pomegranate peel (PP) extract on different human cancer cell lines. MTT was performed to estimate cytotoxic effects of PP extract against HTB140, HTB177, MCF7, HCT116 human cancer cell lines and MRC-5 normal fibroblasts. Clonogenic assay was used to reveal cell survival after the treatment with PP extract. Cell cycle analysis was done using flow cytometry. Wound healing assay was applied to estimate inhibitory effects of PP extract on migration of cancer cells. The results showed that PP extract expressed selective cytotoxicity for cancer cells compared to normal cell line. Analyzed cancer cell lines displayed individual variations in sensitivity to PP extract reflected through changes in clonogenic survival, cell cycle distribution and migration, which may be due to the specific nature of each tested cell line. In conclusion, PP extract exhibits good inhibitory effects on tested cancer cell lines.
T2  - Records of Natural Products
T1  - Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines
VL  - 14
IS  - 5
SP  - 326
EP  - 339
DO  - 10.25135/rnp.170.19.11.1477
ER  - 

@article{
author = "Keta, Otilija D. and Deljanin, Milena and Petković, Vladana and Zdunić, Gordana and Janković, Teodora and Živković, Jelena and Ristić-Fira, Aleksandra and Petrović, Ivan M. and Šavikin, Katarina",
year = "2020",
abstract = "The aim of the present study was to investigate effects of pomegranate peel (PP) extract on different human cancer cell lines. MTT was performed to estimate cytotoxic effects of PP extract against HTB140, HTB177, MCF7, HCT116 human cancer cell lines and MRC-5 normal fibroblasts. Clonogenic assay was used to reveal cell survival after the treatment with PP extract. Cell cycle analysis was done using flow cytometry. Wound healing assay was applied to estimate inhibitory effects of PP extract on migration of cancer cells. The results showed that PP extract expressed selective cytotoxicity for cancer cells compared to normal cell line. Analyzed cancer cell lines displayed individual variations in sensitivity to PP extract reflected through changes in clonogenic survival, cell cycle distribution and migration, which may be due to the specific nature of each tested cell line. In conclusion, PP extract exhibits good inhibitory effects on tested cancer cell lines.",
journal = "Records of Natural Products",
title = "Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines",
volume = "14",
number = "5",
pages = "326-339",
doi = "10.25135/rnp.170.19.11.1477"
}

Keta, O. D., Deljanin, M., Petković, V., Zdunić, G., Janković, T., Živković, J., Ristić-Fira, A., Petrović, I. M.,& Šavikin, K.. (2020). Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines. in Records of Natural Products, 14(5), 326-339.
https://doi.org/10.25135/rnp.170.19.11.1477

Keta OD, Deljanin M, Petković V, Zdunić G, Janković T, Živković J, Ristić-Fira A, Petrović IM, Šavikin K. Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines. in Records of Natural Products. 2020;14(5):326-339.
doi:10.25135/rnp.170.19.11.1477 .

Keta, Otilija D., Deljanin, Milena, Petković, Vladana, Zdunić, Gordana, Janković, Teodora, Živković, Jelena, Ristić-Fira, Aleksandra, Petrović, Ivan M., Šavikin, Katarina, "Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines" in Records of Natural Products, 14, no. 5 (2020):326-339,
https://doi.org/10.25135/rnp.170.19.11.1477 . .

TY  - JOUR
AU  - Petković, Vladana
AU  - Keta, Otilija D.
AU  - Vidosavljević, Marija Z.
AU  - Incerti, Sebastien
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8414
AB  - Purpose: Investigation of effects on DNA of γ-irradiated human cancer cells pretreated with free radical scavengers is aimed to create reference data which would enable assessment of the relative efficiency of high linear energy transfer (LET) radiations used in hadron therapy, i.e. protons and carbon ions. Materials and methods: MCF-7 breast and HTB177 lung cancer cells are irradiated with γ-rays. To minimize indirect effects of irradiation, dimethyl sulfoxide (DMSO) or glycerol are applied as free radical scavengers. Biological response to irradiation is evaluated through clonogenic cell survival, immunocytochemical and cell cycle analysis, as well as expression of proteins involved in DNA damage response. Results: Examined cell lines reveal similar level of radioresistance. Application of scavengers leads to the rise of cell survival and decreases the number of DNA double strand breaks in irradiated cells. Differences in cell cycle and protein expression between the two cell lines are probably caused by different DNA damage repair mechanisms that are activated. Conclusion: The obtained results show that DMSO and glycerol have good scavenging capacity, and may be used to minimize DNA damage induced by free radicals. Therefore, they will be used as the reference for comparison with high LET irradiations, as well as good experimental data suitable for validation of numerical simulations. © 2019, © 2019 Taylor & Francis Group, LLC.
T2  - International Journal of Radiation Biology
T1  - Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers
VL  - 95
IS  - 3
SP  - 274
EP  - 285
DO  - 10.1080/09553002.2019.1549753
ER  - 

@article{
author = "Petković, Vladana and Keta, Otilija D. and Vidosavljević, Marija Z. and Incerti, Sebastien and Ristić-Fira, Aleksandra and Petrović, Ivan M.",
year = "2019",
abstract = "Purpose: Investigation of effects on DNA of γ-irradiated human cancer cells pretreated with free radical scavengers is aimed to create reference data which would enable assessment of the relative efficiency of high linear energy transfer (LET) radiations used in hadron therapy, i.e. protons and carbon ions. Materials and methods: MCF-7 breast and HTB177 lung cancer cells are irradiated with γ-rays. To minimize indirect effects of irradiation, dimethyl sulfoxide (DMSO) or glycerol are applied as free radical scavengers. Biological response to irradiation is evaluated through clonogenic cell survival, immunocytochemical and cell cycle analysis, as well as expression of proteins involved in DNA damage response. Results: Examined cell lines reveal similar level of radioresistance. Application of scavengers leads to the rise of cell survival and decreases the number of DNA double strand breaks in irradiated cells. Differences in cell cycle and protein expression between the two cell lines are probably caused by different DNA damage repair mechanisms that are activated. Conclusion: The obtained results show that DMSO and glycerol have good scavenging capacity, and may be used to minimize DNA damage induced by free radicals. Therefore, they will be used as the reference for comparison with high LET irradiations, as well as good experimental data suitable for validation of numerical simulations. © 2019, © 2019 Taylor & Francis Group, LLC.",
journal = "International Journal of Radiation Biology",
title = "Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers",
volume = "95",
number = "3",
pages = "274-285",
doi = "10.1080/09553002.2019.1549753"
}

Petković, V., Keta, O. D., Vidosavljević, M. Z., Incerti, S., Ristić-Fira, A.,& Petrović, I. M.. (2019). Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers. in International Journal of Radiation Biology, 95(3), 274-285.
https://doi.org/10.1080/09553002.2019.1549753

Petković V, Keta OD, Vidosavljević MZ, Incerti S, Ristić-Fira A, Petrović IM. Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers. in International Journal of Radiation Biology. 2019;95(3):274-285.
doi:10.1080/09553002.2019.1549753 .

Petković, Vladana, Keta, Otilija D., Vidosavljević, Marija Z., Incerti, Sebastien, Ristić-Fira, Aleksandra, Petrović, Ivan M., "Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers" in International Journal of Radiation Biology, 95, no. 3 (2019):274-285,
https://doi.org/10.1080/09553002.2019.1549753 . .

TY  - CONF
AU  - Petković, Vladana
AU  - Keta, Otilija D.
AU  - Vojinović, N.
AU  - Incerti, Sebastien
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9207
AB  - Direct  effects  of  radiation  affect  the  DNA  molecule,  causing  DNAdamage and finally  cell death. We examined the role of DMSO and glycerol as free-radical scavengers in HTB177 cells irradiated with gamma rays. Direct effects of   radiation   were   estimated   through   DNA   double   strand   break   (DSB) quantification  and  cell  survival.  Results  of  this  work  revealed  that  chosen concentration   of   DMSO   exhibit   higher   protective   effect   comparing   to glycerol.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
T1  - Radio-protective effect of DMSO glycerol in human non-small cell lung cancer irradiated with gamma rays
SP  - 447
EP  - 450
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9207
ER  - 

@conference{
author = "Petković, Vladana and Keta, Otilija D. and Vojinović, N. and Incerti, Sebastien and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2016",
abstract = "Direct  effects  of  radiation  affect  the  DNA  molecule,  causing  DNAdamage and finally  cell death. We examined the role of DMSO and glycerol as free-radical scavengers in HTB177 cells irradiated with gamma rays. Direct effects of   radiation   were   estimated   through   DNA   double   strand   break   (DSB) quantification  and  cell  survival.  Results  of  this  work  revealed  that  chosen concentration   of   DMSO   exhibit   higher   protective   effect   comparing   to glycerol.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry",
title = "Radio-protective effect of DMSO glycerol in human non-small cell lung cancer irradiated with gamma rays",
pages = "447-450",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9207"
}

Petković, V., Keta, O. D., Vojinović, N., Incerti, S., Petrović, I. M.,& Ristić-Fira, A.. (2016). Radio-protective effect of DMSO glycerol in human non-small cell lung cancer irradiated with gamma rays. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 447-450.
https://hdl.handle.net/21.15107/rcub_vinar_9207

Petković V, Keta OD, Vojinović N, Incerti S, Petrović IM, Ristić-Fira A. Radio-protective effect of DMSO glycerol in human non-small cell lung cancer irradiated with gamma rays. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry. 2016;:447-450.
https://hdl.handle.net/21.15107/rcub_vinar_9207 .

Petković, Vladana, Keta, Otilija D., Vojinović, N., Incerti, Sebastien, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Radio-protective effect of DMSO glycerol in human non-small cell lung cancer irradiated with gamma rays" in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry (2016):447-450,
https://hdl.handle.net/21.15107/rcub_vinar_9207 .