TY  - JOUR
AU  - Stojković, Goran
AU  - Jovanović, Ivan G.
AU  - Dimitrijević, Milovan
AU  - Jovanović, Jasmina
AU  - Tomanović, Nada
AU  - Stanković, Aleksandra
AU  - Arsović, Nenad
AU  - Boričić, Ivan
AU  - Zeljić, Katarina
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10217
AB  - Objectives This study aimed to experimentally validate dysregulated expression of miRNA candidates selected through updated meta-analysis of most commonly deregulated miRNAs in oral cancer and to explore their diagnostic and prognostic potential. Materials and methods Five miRNAs (miR-31-3p, miR-135b-5p, miR-18a-5p, miR-30a-5p and miR-139-5p) from updated meta-signature were selected for validation by qRT-PCR method in 35 oral cancer clinical specimens and adjacent non-cancerous tissue. Results Updated meta-analysis has identified 13 most commonly deregulated miRNAs in oral cancer. Seven miRNAs were consistently up-regulated (miR-21-5p, miR-31-3p, miR-135b-5p, miR-31-5p, miR-424-5p, miR-18a-5p and miR-21-3p), while five were down-regulated (miR-139-5p, miR-30a-3p, miR-375-3p, miR-376c-3p and miR-30a-5p). Increased expression of miR-31-3p and miR-135b-5p, and decreased expression of miR-139-5p and miR-30a-5p were confirmed in oral cancer compared to adjacent non-cancerous tissue. A three miRNAs combination (miR-31-3p, miR-139-5p and miR-30a-5p) gave the most promising diagnostic potential for discriminating oral cancer from non-cancerous tissue (AUC: 0.780 [95% CI: 0.673–0.886], p < 0.0005, sensitivity 94.3%, specificity 51.4%). High expression of miR-135b-5p, miR-18a-5p and miR-30a-5p was associated with poor survival (p = 0.003, p = 0.048, p = 0.016 respectively). Conclusion miR-31-3p, miR-139-5p and miR-30a-5p panel was confirmed as a potential diagnostic biomarker when distinguishing oral cancer from non-cancerous tissue. miR-135b-5p, miR-18a-5p and miR-30a-5p might serve as potential biomarkers of poor survival of oral cancer patients.
T2  - Oral Diseases
T1  - Meta-signature guided investigation of miRNA candidates as potential biomarkers of oral cancer
VL  - inpress
SP  - 1
EP  - 15
DO  - 10.1111/odi.14185
ER  - 

@article{
author = "Stojković, Goran and Jovanović, Ivan G. and Dimitrijević, Milovan and Jovanović, Jasmina and Tomanović, Nada and Stanković, Aleksandra and Arsović, Nenad and Boričić, Ivan and Zeljić, Katarina",
year = "2022",
abstract = "Objectives This study aimed to experimentally validate dysregulated expression of miRNA candidates selected through updated meta-analysis of most commonly deregulated miRNAs in oral cancer and to explore their diagnostic and prognostic potential. Materials and methods Five miRNAs (miR-31-3p, miR-135b-5p, miR-18a-5p, miR-30a-5p and miR-139-5p) from updated meta-signature were selected for validation by qRT-PCR method in 35 oral cancer clinical specimens and adjacent non-cancerous tissue. Results Updated meta-analysis has identified 13 most commonly deregulated miRNAs in oral cancer. Seven miRNAs were consistently up-regulated (miR-21-5p, miR-31-3p, miR-135b-5p, miR-31-5p, miR-424-5p, miR-18a-5p and miR-21-3p), while five were down-regulated (miR-139-5p, miR-30a-3p, miR-375-3p, miR-376c-3p and miR-30a-5p). Increased expression of miR-31-3p and miR-135b-5p, and decreased expression of miR-139-5p and miR-30a-5p were confirmed in oral cancer compared to adjacent non-cancerous tissue. A three miRNAs combination (miR-31-3p, miR-139-5p and miR-30a-5p) gave the most promising diagnostic potential for discriminating oral cancer from non-cancerous tissue (AUC: 0.780 [95% CI: 0.673–0.886], p < 0.0005, sensitivity 94.3%, specificity 51.4%). High expression of miR-135b-5p, miR-18a-5p and miR-30a-5p was associated with poor survival (p = 0.003, p = 0.048, p = 0.016 respectively). Conclusion miR-31-3p, miR-139-5p and miR-30a-5p panel was confirmed as a potential diagnostic biomarker when distinguishing oral cancer from non-cancerous tissue. miR-135b-5p, miR-18a-5p and miR-30a-5p might serve as potential biomarkers of poor survival of oral cancer patients.",
journal = "Oral Diseases",
title = "Meta-signature guided investigation of miRNA candidates as potential biomarkers of oral cancer",
volume = "inpress",
pages = "1-15",
doi = "10.1111/odi.14185"
}

Stojković, G., Jovanović, I. G., Dimitrijević, M., Jovanović, J., Tomanović, N., Stanković, A., Arsović, N., Boričić, I.,& Zeljić, K.. (2022). Meta-signature guided investigation of miRNA candidates as potential biomarkers of oral cancer. in Oral Diseases, inpress, 1-15.
https://doi.org/10.1111/odi.14185

Stojković G, Jovanović IG, Dimitrijević M, Jovanović J, Tomanović N, Stanković A, Arsović N, Boričić I, Zeljić K. Meta-signature guided investigation of miRNA candidates as potential biomarkers of oral cancer. in Oral Diseases. 2022;inpress:1-15.
doi:10.1111/odi.14185 .

Stojković, Goran, Jovanović, Ivan G., Dimitrijević, Milovan, Jovanović, Jasmina, Tomanović, Nada, Stanković, Aleksandra, Arsović, Nenad, Boričić, Ivan, Zeljić, Katarina, "Meta-signature guided investigation of miRNA candidates as potential biomarkers of oral cancer" in Oral Diseases, inpress (2022):1-15,
https://doi.org/10.1111/odi.14185 . .

TY  - CONF
AU  - Stojković, Goran
AU  - Jovanović, Ivan G.
AU  - Stanković, Aleksandra
AU  - Dimitrijević, Milovan
AU  - Zeljić, Katarina
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11015
AB  - Oral cancer is the most prevalent type of head and neck cancer, characterized by rising incidence, high relapse occurrence and low survival. There is a high demand for the identification of novel and sensitive diagnostic and prognostic molecular biomarkers of oral cancer. MicroRNAs (miRNAs) are considered as promising candidates. Previously conducted meta-analysis on high throughput miRNA profiling in oral cancer emphasized consistent down-regulation of miR-30a-5p and miR-139-5p. We aimed to validate these findings in oral cancer clinical samples and to test the diagnostic and prognostic potential of these miRNAs. Bioinformatical prediction of investigated miRNAs target genes was executed through the intersection of multiple miRNA target prediction algorithms. Enrichment analysis of miRNA target genes was performed using miRPathDB V2.0 software on GO:BP database. RNA was isolated from oral cancer and adjacent noncancerous tissue from 30 patients by miRVana kit. cDNA was synthesized by TaqMan microRNA reverse transcription kit. TaqMan gene expression kit was used for relative quantification of miRNAs normalized to RNU6B. Relative expression was calculated by comparative Ct method. The diagnostic potential was estimated by the ROC curve analysis. Expression of miR-30a-5p and miR-139-5p was dichotomized as high or low by the median. Association with clinicopathological features and miRNAs expression was calculated by χ2 test. Bioinformatical analysis demonstrated both enrichment of miR-30a-5p and miR-139-5p targets in biological processes associated with cancer pathology and complementarity in regulation of these processes thus ensuring non-redundant regulation. Both miRNA were significantly down-regulated in oral cancer compared to non-cancerous tissue (Wilcoxon test: p=0.0004, p=0.001, respectively). According to the ROC curve analysis, both miRNAs might be used as potential tools for discrimination between cancerous and non-cancerous tissue (miR-30a-5p AUC=0.677, p=0.019; miR-139-5p AUC=0.656, p=0.038). There was a significant correlation between the expression of miR-30a-5p and miR-139-5p in cancerous tissue (Spearman rank test r=0.901, p<0.0001). None of the analyzed miRNAs was associated with stage, nodal status, tumour size and overall survival (p>0.05) which indicates that miR-30a-5p and miR-139-5p can’t be used as prognostic biomarkers of oral cancer. Our results suggest that miR-30a-5p and miR-139-5p might be used as good diagnostic biomarkers for discrimination between oral cancer and non-cancerous tissue.
PB  - Department of Biology and Ecology : Faculty of Sciences University of Novi Sad
C3  - Biologia Serbica : Belgrade BioInformatics Conference : BelBi2021 : program and the book of abstracts; June 21-25
T1  - Bioinformatic characterization and validation of miR-30a-5p and miR-139-5p as diagnostic and prognostic biomarkers of oral cancer
VL  - 43
IS  - 1
SP  - 102
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11015
ER  - 

@conference{
author = "Stojković, Goran and Jovanović, Ivan G. and Stanković, Aleksandra and Dimitrijević, Milovan and Zeljić, Katarina",
year = "2021",
abstract = "Oral cancer is the most prevalent type of head and neck cancer, characterized by rising incidence, high relapse occurrence and low survival. There is a high demand for the identification of novel and sensitive diagnostic and prognostic molecular biomarkers of oral cancer. MicroRNAs (miRNAs) are considered as promising candidates. Previously conducted meta-analysis on high throughput miRNA profiling in oral cancer emphasized consistent down-regulation of miR-30a-5p and miR-139-5p. We aimed to validate these findings in oral cancer clinical samples and to test the diagnostic and prognostic potential of these miRNAs. Bioinformatical prediction of investigated miRNAs target genes was executed through the intersection of multiple miRNA target prediction algorithms. Enrichment analysis of miRNA target genes was performed using miRPathDB V2.0 software on GO:BP database. RNA was isolated from oral cancer and adjacent noncancerous tissue from 30 patients by miRVana kit. cDNA was synthesized by TaqMan microRNA reverse transcription kit. TaqMan gene expression kit was used for relative quantification of miRNAs normalized to RNU6B. Relative expression was calculated by comparative Ct method. The diagnostic potential was estimated by the ROC curve analysis. Expression of miR-30a-5p and miR-139-5p was dichotomized as high or low by the median. Association with clinicopathological features and miRNAs expression was calculated by χ2 test. Bioinformatical analysis demonstrated both enrichment of miR-30a-5p and miR-139-5p targets in biological processes associated with cancer pathology and complementarity in regulation of these processes thus ensuring non-redundant regulation. Both miRNA were significantly down-regulated in oral cancer compared to non-cancerous tissue (Wilcoxon test: p=0.0004, p=0.001, respectively). According to the ROC curve analysis, both miRNAs might be used as potential tools for discrimination between cancerous and non-cancerous tissue (miR-30a-5p AUC=0.677, p=0.019; miR-139-5p AUC=0.656, p=0.038). There was a significant correlation between the expression of miR-30a-5p and miR-139-5p in cancerous tissue (Spearman rank test r=0.901, p<0.0001). None of the analyzed miRNAs was associated with stage, nodal status, tumour size and overall survival (p>0.05) which indicates that miR-30a-5p and miR-139-5p can’t be used as prognostic biomarkers of oral cancer. Our results suggest that miR-30a-5p and miR-139-5p might be used as good diagnostic biomarkers for discrimination between oral cancer and non-cancerous tissue.",
publisher = "Department of Biology and Ecology : Faculty of Sciences University of Novi Sad",
journal = "Biologia Serbica : Belgrade BioInformatics Conference : BelBi2021 : program and the book of abstracts; June 21-25",
title = "Bioinformatic characterization and validation of miR-30a-5p and miR-139-5p as diagnostic and prognostic biomarkers of oral cancer",
volume = "43",
number = "1",
pages = "102",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11015"
}

Stojković, G., Jovanović, I. G., Stanković, A., Dimitrijević, M.,& Zeljić, K.. (2021). Bioinformatic characterization and validation of miR-30a-5p and miR-139-5p as diagnostic and prognostic biomarkers of oral cancer. in Biologia Serbica : Belgrade BioInformatics Conference : BelBi2021 : program and the book of abstracts; June 21-25
Department of Biology and Ecology : Faculty of Sciences University of Novi Sad., 43(1), 102.
https://hdl.handle.net/21.15107/rcub_vinar_11015

Stojković G, Jovanović IG, Stanković A, Dimitrijević M, Zeljić K. Bioinformatic characterization and validation of miR-30a-5p and miR-139-5p as diagnostic and prognostic biomarkers of oral cancer. in Biologia Serbica : Belgrade BioInformatics Conference : BelBi2021 : program and the book of abstracts; June 21-25. 2021;43(1):102.
https://hdl.handle.net/21.15107/rcub_vinar_11015 .

Stojković, Goran, Jovanović, Ivan G., Stanković, Aleksandra, Dimitrijević, Milovan, Zeljić, Katarina, "Bioinformatic characterization and validation of miR-30a-5p and miR-139-5p as diagnostic and prognostic biomarkers of oral cancer" in Biologia Serbica : Belgrade BioInformatics Conference : BelBi2021 : program and the book of abstracts; June 21-25, 43, no. 1 (2021):102,
https://hdl.handle.net/21.15107/rcub_vinar_11015 .