TY  - JOUR
AU  - Zmejkoski, Danica
AU  - Zdravković, Nemanja M.
AU  - Budimir Filimonović, Milica D.
AU  - Pavlović, Vladimir B.
AU  - Butulija, Svetlana
AU  - Milivojević, Dušan
AU  - Marković, Zoran M.
AU  - Todorović Marković, Biljana M.
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12990
AB  - In this study, nanochitosan dots (ChiDs) were synthesized using gamma rays and encapsulated in bacterial cellulose (BC) polymer matrix for antibiofilm potential in photodynamic therapy. The composites were analyzed for structural changes using SEM, AFM, FTIR, XRD, EPR, and porosity measurements. Additionally, ChiD release was assessed. The results showed that the chemical composition remained unaltered, but ChiD agglomerates embedded in BC changed shape (1.5–2.5 µm). Bacterial cellulose fibers became deformed and interconnected, with increased surface roughness and porosity and decreased crystallinity. No singlet oxygen formation was observed, and the total amount of released ChiD was up to 16.10%. Antibiofilm activity was higher under green light, with reductions ranging from 48 to 57% under blue light and 78 to 85% under green light. Methicillin-resistant Staphylococcus aureus was the most sensitive strain. The new photoactive composite hydrogels show promising potential for combating biofilm-related infections.
T2  - Journal of Functional Biomaterials
T1  - Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light
VL  - 15
IS  - 3
SP  - 72
DO  - 10.3390/jfb15030072
ER  - 

@article{
author = "Zmejkoski, Danica and Zdravković, Nemanja M. and Budimir Filimonović, Milica D. and Pavlović, Vladimir B. and Butulija, Svetlana and Milivojević, Dušan and Marković, Zoran M. and Todorović Marković, Biljana M.",
year = "2024",
abstract = "In this study, nanochitosan dots (ChiDs) were synthesized using gamma rays and encapsulated in bacterial cellulose (BC) polymer matrix for antibiofilm potential in photodynamic therapy. The composites were analyzed for structural changes using SEM, AFM, FTIR, XRD, EPR, and porosity measurements. Additionally, ChiD release was assessed. The results showed that the chemical composition remained unaltered, but ChiD agglomerates embedded in BC changed shape (1.5–2.5 µm). Bacterial cellulose fibers became deformed and interconnected, with increased surface roughness and porosity and decreased crystallinity. No singlet oxygen formation was observed, and the total amount of released ChiD was up to 16.10%. Antibiofilm activity was higher under green light, with reductions ranging from 48 to 57% under blue light and 78 to 85% under green light. Methicillin-resistant Staphylococcus aureus was the most sensitive strain. The new photoactive composite hydrogels show promising potential for combating biofilm-related infections.",
journal = "Journal of Functional Biomaterials",
title = "Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light",
volume = "15",
number = "3",
pages = "72",
doi = "10.3390/jfb15030072"
}

Zmejkoski, D., Zdravković, N. M., Budimir Filimonović, M. D., Pavlović, V. B., Butulija, S., Milivojević, D., Marković, Z. M.,& Todorović Marković, B. M.. (2024). Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light. in Journal of Functional Biomaterials, 15(3), 72.
https://doi.org/10.3390/jfb15030072

Zmejkoski D, Zdravković NM, Budimir Filimonović MD, Pavlović VB, Butulija S, Milivojević D, Marković ZM, Todorović Marković BM. Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light. in Journal of Functional Biomaterials. 2024;15(3):72.
doi:10.3390/jfb15030072 .

Zmejkoski, Danica, Zdravković, Nemanja M., Budimir Filimonović, Milica D., Pavlović, Vladimir B., Butulija, Svetlana, Milivojević, Dušan, Marković, Zoran M., Todorović Marković, Biljana M., "Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light" in Journal of Functional Biomaterials, 15, no. 3 (2024):72,
https://doi.org/10.3390/jfb15030072 . .

TY  - JOUR
AU  - Bulat, Tanja
AU  - Zmejkoski, Danica
AU  - Marković, Zoran
AU  - Satzinger, Melissa
AU  - Kovač, Janez
AU  - Strobl, Birgit
AU  - Milivojević, Dušan
AU  - Todorović Marković, Biljana
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11831
AB  - Carbon quantum dots (CQDs) are well known as a promising therapeutic agent due to their excellent pro-oxidant, antioxidant, antibacterial and anticancer properties under visible light irradiation. This material is easy to produce by several bottom-up or top-down methods. In this study, we used citric acid as starting precursor to synthesize aqueous carbon quantum dots dispersion by pyrolysis at 210 ◦C. Atomic force microscopy and XPS analyses revealed that CQDs synthesized are quasi-spherical with typical diameter of 9 nm and lots of C–O and C––O functional groups distributed over the basal plane and edges of the dots. Here, we investigated the antiviral properties of CQDs against cytomegalovirus (CMV). The pre-treatment with CQDs upregulates interferonstimulated genes (ISG), resulting in better virus control. Cellular defence against cytomegalovirus of CQDs pre-treated cells is increased in a dose-dependent manner. Our results reveal high biocompatibility and potent in vitro antiviral properties of CQDs.
T2  - Materials Chemistry and Physics
T1  - Employing carbon quantum dots to combat cytomegalovirus
VL  - 311
SP  - 128495
DO  - 10.1016/j.matchemphys.2023.128495
ER  - 

@article{
author = "Bulat, Tanja and Zmejkoski, Danica and Marković, Zoran and Satzinger, Melissa and Kovač, Janez and Strobl, Birgit and Milivojević, Dušan and Todorović Marković, Biljana",
year = "2024",
abstract = "Carbon quantum dots (CQDs) are well known as a promising therapeutic agent due to their excellent pro-oxidant, antioxidant, antibacterial and anticancer properties under visible light irradiation. This material is easy to produce by several bottom-up or top-down methods. In this study, we used citric acid as starting precursor to synthesize aqueous carbon quantum dots dispersion by pyrolysis at 210 ◦C. Atomic force microscopy and XPS analyses revealed that CQDs synthesized are quasi-spherical with typical diameter of 9 nm and lots of C–O and C––O functional groups distributed over the basal plane and edges of the dots. Here, we investigated the antiviral properties of CQDs against cytomegalovirus (CMV). The pre-treatment with CQDs upregulates interferonstimulated genes (ISG), resulting in better virus control. Cellular defence against cytomegalovirus of CQDs pre-treated cells is increased in a dose-dependent manner. Our results reveal high biocompatibility and potent in vitro antiviral properties of CQDs.",
journal = "Materials Chemistry and Physics",
title = "Employing carbon quantum dots to combat cytomegalovirus",
volume = "311",
pages = "128495",
doi = "10.1016/j.matchemphys.2023.128495"
}

Bulat, T., Zmejkoski, D., Marković, Z., Satzinger, M., Kovač, J., Strobl, B., Milivojević, D.,& Todorović Marković, B.. (2024). Employing carbon quantum dots to combat cytomegalovirus. in Materials Chemistry and Physics, 311, 128495.
https://doi.org/10.1016/j.matchemphys.2023.128495

Bulat T, Zmejkoski D, Marković Z, Satzinger M, Kovač J, Strobl B, Milivojević D, Todorović Marković B. Employing carbon quantum dots to combat cytomegalovirus. in Materials Chemistry and Physics. 2024;311:128495.
doi:10.1016/j.matchemphys.2023.128495 .

Bulat, Tanja, Zmejkoski, Danica, Marković, Zoran, Satzinger, Melissa, Kovač, Janez, Strobl, Birgit, Milivojević, Dušan, Todorović Marković, Biljana, "Employing carbon quantum dots to combat cytomegalovirus" in Materials Chemistry and Physics, 311 (2024):128495,
https://doi.org/10.1016/j.matchemphys.2023.128495 . .

TY  - JOUR
AU  - Marković, Zoran M.
AU  - Budimir, Milica
AU  - Danko, Martin
AU  - Milivojević, Dušan
AU  - Kubat, Pavel
AU  - Zmejkoski, Danica
AU  - Pavlović, Vladimir B.
AU  - Mojsin, Marija
AU  - Stevanović, Milena J.
AU  - Todorović-Marković, Biljana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10632
AB  - Carbon quantum dots as a novel type of carbon nanomaterials have attracted the attention of many researchers because of their unique optical, antibacterial, and anticancer properties as well as their biocompatibility. In this study, for the first time, carbon quantum dots were prepared from o-phenylenediamine dissolved in toluene by a solvothermal route. Subsequently, the prepared carbon quantum dots were encapsulated into polyurethane films by a swelling–encapsulation–shrink method. Analyses of the results obtained by different characterization methods (AFM, TEM, EDS, FTIR, photoluminescence, and EPR) indicate the significant influence of the precursor on structural, chemical, and optical properties. Antibacterial and cytotoxicity tests showed that these dots did not have any antibacterial potential, because of the low extent of reactive oxygen species production, and showed low dark cytotoxicity. By investigating the cellular uptake, it was established that these dots penetrated the HeLa cells and could be used as probes for bioimaging.
T2  - Beilstein Journal of Nanotechnology
T1  - Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine
VL  - 14
IS  - 1
SP  - 165
EP  - 174
DO  - 10.3762/bjnano.14.17
ER  - 

@article{
author = "Marković, Zoran M. and Budimir, Milica and Danko, Martin and Milivojević, Dušan and Kubat, Pavel and Zmejkoski, Danica and Pavlović, Vladimir B. and Mojsin, Marija and Stevanović, Milena J. and Todorović-Marković, Biljana",
year = "2023",
abstract = "Carbon quantum dots as a novel type of carbon nanomaterials have attracted the attention of many researchers because of their unique optical, antibacterial, and anticancer properties as well as their biocompatibility. In this study, for the first time, carbon quantum dots were prepared from o-phenylenediamine dissolved in toluene by a solvothermal route. Subsequently, the prepared carbon quantum dots were encapsulated into polyurethane films by a swelling–encapsulation–shrink method. Analyses of the results obtained by different characterization methods (AFM, TEM, EDS, FTIR, photoluminescence, and EPR) indicate the significant influence of the precursor on structural, chemical, and optical properties. Antibacterial and cytotoxicity tests showed that these dots did not have any antibacterial potential, because of the low extent of reactive oxygen species production, and showed low dark cytotoxicity. By investigating the cellular uptake, it was established that these dots penetrated the HeLa cells and could be used as probes for bioimaging.",
journal = "Beilstein Journal of Nanotechnology",
title = "Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine",
volume = "14",
number = "1",
pages = "165-174",
doi = "10.3762/bjnano.14.17"
}

Marković, Z. M., Budimir, M., Danko, M., Milivojević, D., Kubat, P., Zmejkoski, D., Pavlović, V. B., Mojsin, M., Stevanović, M. J.,& Todorović-Marković, B.. (2023). Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine. in Beilstein Journal of Nanotechnology, 14(1), 165-174.
https://doi.org/10.3762/bjnano.14.17

Marković ZM, Budimir M, Danko M, Milivojević D, Kubat P, Zmejkoski D, Pavlović VB, Mojsin M, Stevanović MJ, Todorović-Marković B. Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine. in Beilstein Journal of Nanotechnology. 2023;14(1):165-174.
doi:10.3762/bjnano.14.17 .

Marković, Zoran M., Budimir, Milica, Danko, Martin, Milivojević, Dušan, Kubat, Pavel, Zmejkoski, Danica, Pavlović, Vladimir B., Mojsin, Marija, Stevanović, Milena J., Todorović-Marković, Biljana, "Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine" in Beilstein Journal of Nanotechnology, 14, no. 1 (2023):165-174,
https://doi.org/10.3762/bjnano.14.17 . .

TY  - JOUR
AU  - Jovanović, Svetlana P.
AU  - Marković, Zoran M.
AU  - Budimir, Milica
AU  - Prekodravac, Jovana
AU  - Zmejkoski, Danica
AU  - Kepić, Dejan
AU  - Bonasera, Aurelio
AU  - Todorović-Marković, Biljana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10926
AB  - The large number of deaths induced by carcinoma and infections indicates that the need for new, better, targeted therapy is higher than ever. Apart from classical treatments and medication, photodynamic therapy (PDT) is one of the possible approaches to cure these clinical conditions. This strategy offers several advantages, such as lower toxicity, selective treatment, faster recovery time, avoidance of systemic toxic effects, and others. Unfortunately, there is a small number of agents that are approved for usage in clinical PDT. Novel, efficient, biocompatible PDT agents are, thus, highly desired. One of the most promising candidates is represented by the broad family of carbon-based quantum dots, such as graphene quantum dots (GQDs), carbon quantum dots (CQDs), carbon nanodots (CNDs), and carbonized polymer dots (CPDs). In this review paper, these new smart nanomaterials are discussed as potential PDT agents, detailing their toxicity in the dark, and when they are exposed to light, as well as their effects on carcinoma and bacterial cells. The photoinduced effects of carbon-based quantum dots on bacteria and viruses are particularly interesting, since dots usually generate several highly toxic reactive oxygen species under blue light. These species are acting as bombs on pathogen cells, causing various devastating and toxic effects on those targets.
T2  - Pharmaceutics
T1  - Lights and Dots toward Therapy—Carbon-Based Quantum Dots as New Agents for Photodynamic Therapy
VL  - 15
IS  - 4
SP  - 1170
DO  - 10.3390/pharmaceutics15041170
ER  - 

@article{
author = "Jovanović, Svetlana P. and Marković, Zoran M. and Budimir, Milica and Prekodravac, Jovana and Zmejkoski, Danica and Kepić, Dejan and Bonasera, Aurelio and Todorović-Marković, Biljana",
year = "2023",
abstract = "The large number of deaths induced by carcinoma and infections indicates that the need for new, better, targeted therapy is higher than ever. Apart from classical treatments and medication, photodynamic therapy (PDT) is one of the possible approaches to cure these clinical conditions. This strategy offers several advantages, such as lower toxicity, selective treatment, faster recovery time, avoidance of systemic toxic effects, and others. Unfortunately, there is a small number of agents that are approved for usage in clinical PDT. Novel, efficient, biocompatible PDT agents are, thus, highly desired. One of the most promising candidates is represented by the broad family of carbon-based quantum dots, such as graphene quantum dots (GQDs), carbon quantum dots (CQDs), carbon nanodots (CNDs), and carbonized polymer dots (CPDs). In this review paper, these new smart nanomaterials are discussed as potential PDT agents, detailing their toxicity in the dark, and when they are exposed to light, as well as their effects on carcinoma and bacterial cells. The photoinduced effects of carbon-based quantum dots on bacteria and viruses are particularly interesting, since dots usually generate several highly toxic reactive oxygen species under blue light. These species are acting as bombs on pathogen cells, causing various devastating and toxic effects on those targets.",
journal = "Pharmaceutics",
title = "Lights and Dots toward Therapy—Carbon-Based Quantum Dots as New Agents for Photodynamic Therapy",
volume = "15",
number = "4",
pages = "1170",
doi = "10.3390/pharmaceutics15041170"
}

Jovanović, S. P., Marković, Z. M., Budimir, M., Prekodravac, J., Zmejkoski, D., Kepić, D., Bonasera, A.,& Todorović-Marković, B.. (2023). Lights and Dots toward Therapy—Carbon-Based Quantum Dots as New Agents for Photodynamic Therapy. in Pharmaceutics, 15(4), 1170.
https://doi.org/10.3390/pharmaceutics15041170

Jovanović SP, Marković ZM, Budimir M, Prekodravac J, Zmejkoski D, Kepić D, Bonasera A, Todorović-Marković B. Lights and Dots toward Therapy—Carbon-Based Quantum Dots as New Agents for Photodynamic Therapy. in Pharmaceutics. 2023;15(4):1170.
doi:10.3390/pharmaceutics15041170 .

Jovanović, Svetlana P., Marković, Zoran M., Budimir, Milica, Prekodravac, Jovana, Zmejkoski, Danica, Kepić, Dejan, Bonasera, Aurelio, Todorović-Marković, Biljana, "Lights and Dots toward Therapy—Carbon-Based Quantum Dots as New Agents for Photodynamic Therapy" in Pharmaceutics, 15, no. 4 (2023):1170,
https://doi.org/10.3390/pharmaceutics15041170 . .

TY  - JOUR
AU  - Marković, Zoran M.
AU  - Mišović, Aleksandra
AU  - Zmejkoski, Danica
AU  - Zdravković, Nemanja M.
AU  - Kovač, Janez
AU  - Bajuk-Bogdanović, Danica
AU  - Milivojević, Dušan
AU  - Mojsin, Marija
AU  - Stevanović, Milena
AU  - Pavlović, Vladimir B.
AU  - Todorović-Marković, Biljana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11075
AB  - Nowadays, it is a great challenge to develop new medicines for treating various infectious diseases. The treatment of these diseases is of utmost interest to further prevent the development of multi-drug resistance in different pathogens. Carbon quantum dots, as a new member of the carbon nanomaterials family, can potentially be used as a highly promising visible-light-triggered antibacterial agent. In this work, the results of antibacterial and cytotoxic activities of gamma-ray-irradiated carbon quantum dots are presented. Carbon quantum dots (CQDs) were synthesized from citric acid by a pyrolysis procedure and irradiated by gamma rays at different doses (25, 50, 100 and 200 kGy). Structure, chemical composition and optical properties were investigated by atomic force microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, UV-Vis spectrometry and photoluminescence. Structural analysis showed that CQDs have a spherical-like shape and dose-dependent average diameters and heights. Antibacterial tests showed that all irradiated dots had antibacterial activity but CQDs irradiated with dose of 100 kGy had antibacterial activity against all seven pathogen-reference bacterial strains. Gamma-ray-modified CQDs did not show any cytotoxicity toward human fetal-originated MRC-5 cells. Moreover, fluorescence microscopy showed excellent cellular uptake of CQDs irradiated with doses of 25 and 200 kGy into MRC-5 cells.
T2  - Antibiotics
T1  - Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of Bacteria
VL  - 12
IS  - 5
SP  - 919
DO  - 10.3390/antibiotics12050919
ER  - 

@article{
author = "Marković, Zoran M. and Mišović, Aleksandra and Zmejkoski, Danica and Zdravković, Nemanja M. and Kovač, Janez and Bajuk-Bogdanović, Danica and Milivojević, Dušan and Mojsin, Marija and Stevanović, Milena and Pavlović, Vladimir B. and Todorović-Marković, Biljana",
year = "2023",
abstract = "Nowadays, it is a great challenge to develop new medicines for treating various infectious diseases. The treatment of these diseases is of utmost interest to further prevent the development of multi-drug resistance in different pathogens. Carbon quantum dots, as a new member of the carbon nanomaterials family, can potentially be used as a highly promising visible-light-triggered antibacterial agent. In this work, the results of antibacterial and cytotoxic activities of gamma-ray-irradiated carbon quantum dots are presented. Carbon quantum dots (CQDs) were synthesized from citric acid by a pyrolysis procedure and irradiated by gamma rays at different doses (25, 50, 100 and 200 kGy). Structure, chemical composition and optical properties were investigated by atomic force microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, UV-Vis spectrometry and photoluminescence. Structural analysis showed that CQDs have a spherical-like shape and dose-dependent average diameters and heights. Antibacterial tests showed that all irradiated dots had antibacterial activity but CQDs irradiated with dose of 100 kGy had antibacterial activity against all seven pathogen-reference bacterial strains. Gamma-ray-modified CQDs did not show any cytotoxicity toward human fetal-originated MRC-5 cells. Moreover, fluorescence microscopy showed excellent cellular uptake of CQDs irradiated with doses of 25 and 200 kGy into MRC-5 cells.",
journal = "Antibiotics",
title = "Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of Bacteria",
volume = "12",
number = "5",
pages = "919",
doi = "10.3390/antibiotics12050919"
}

Marković, Z. M., Mišović, A., Zmejkoski, D., Zdravković, N. M., Kovač, J., Bajuk-Bogdanović, D., Milivojević, D., Mojsin, M., Stevanović, M., Pavlović, V. B.,& Todorović-Marković, B.. (2023). Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of Bacteria. in Antibiotics, 12(5), 919.
https://doi.org/10.3390/antibiotics12050919

Marković ZM, Mišović A, Zmejkoski D, Zdravković NM, Kovač J, Bajuk-Bogdanović D, Milivojević D, Mojsin M, Stevanović M, Pavlović VB, Todorović-Marković B. Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of Bacteria. in Antibiotics. 2023;12(5):919.
doi:10.3390/antibiotics12050919 .

Marković, Zoran M., Mišović, Aleksandra, Zmejkoski, Danica, Zdravković, Nemanja M., Kovač, Janez, Bajuk-Bogdanović, Danica, Milivojević, Dušan, Mojsin, Marija, Stevanović, Milena, Pavlović, Vladimir B., Todorović-Marković, Biljana, "Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of Bacteria" in Antibiotics, 12, no. 5 (2023):919,
https://doi.org/10.3390/antibiotics12050919 . .

TY  - JOUR
AU  - Spasojević, Dragica
AU  - Zmejkoski, Danica
AU  - Milčić Matić, Natalija
AU  - Krnjaić, Dejan
AU  - Radotić, Ksenija
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11589
AB  - Bacterial infections and resistance to antibiotics are increasingly severe problems. In recent years, Staphylococcus species have emerged as important pathogens in animals and humans. Current therapeutic methods against these species have serious disadvantages; therefore new agents with antibacterial potential, such as plant-based substances, are very important in therapy. We report a pilot study with new method of fractioning the dehydrogenate polymer DHP obtained from coniferyl alcohol and application of the low-MW fractions of 200–3000 Da for antibacterial activity in healing animal lesions. In vivo experiments were conducted on the dogs having a skin lesion. Dogs were treated with the suspension containing the low-MW DHP fractions as the active ingredient, in combination with alginate for 7 days. Cytological smears and microbiological analyses of the affected area were performed. Staphylococcus spp. was isolated from lesions in all dogs from our research. The results show that the low-MW DHP suspension in alginate promotes skin healing and reduction of the infection of the lesions in the affected animals. Pharmaceutical composition containing the low-MW DHP fractions exerts a soothing effect on the subject in wound treatment. Reduction in the number of bacteria by 30% and more were noticed in 6 dogs, while in 4 dogs this percentage is above 50%. No side effects were noticed. Synthesized lignin oligomers may have a significant place as antimicrobial and skin healing agents, especially since an increasing number of multidrug-resistant staphylococci are found on the skin lesions in animals.
T2  - Veterinary Research Communications
T1  - Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study
DO  - 10.1007/s11259-023-10224-9
ER  - 

@article{
author = "Spasojević, Dragica and Zmejkoski, Danica and Milčić Matić, Natalija and Krnjaić, Dejan and Radotić, Ksenija",
year = "2023",
abstract = "Bacterial infections and resistance to antibiotics are increasingly severe problems. In recent years, Staphylococcus species have emerged as important pathogens in animals and humans. Current therapeutic methods against these species have serious disadvantages; therefore new agents with antibacterial potential, such as plant-based substances, are very important in therapy. We report a pilot study with new method of fractioning the dehydrogenate polymer DHP obtained from coniferyl alcohol and application of the low-MW fractions of 200–3000 Da for antibacterial activity in healing animal lesions. In vivo experiments were conducted on the dogs having a skin lesion. Dogs were treated with the suspension containing the low-MW DHP fractions as the active ingredient, in combination with alginate for 7 days. Cytological smears and microbiological analyses of the affected area were performed. Staphylococcus spp. was isolated from lesions in all dogs from our research. The results show that the low-MW DHP suspension in alginate promotes skin healing and reduction of the infection of the lesions in the affected animals. Pharmaceutical composition containing the low-MW DHP fractions exerts a soothing effect on the subject in wound treatment. Reduction in the number of bacteria by 30% and more were noticed in 6 dogs, while in 4 dogs this percentage is above 50%. No side effects were noticed. Synthesized lignin oligomers may have a significant place as antimicrobial and skin healing agents, especially since an increasing number of multidrug-resistant staphylococci are found on the skin lesions in animals.",
journal = "Veterinary Research Communications",
title = "Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study",
doi = "10.1007/s11259-023-10224-9"
}

Spasojević, D., Zmejkoski, D., Milčić Matić, N., Krnjaić, D.,& Radotić, K.. (2023). Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study. in Veterinary Research Communications.
https://doi.org/10.1007/s11259-023-10224-9

Spasojević D, Zmejkoski D, Milčić Matić N, Krnjaić D, Radotić K. Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study. in Veterinary Research Communications. 2023;.
doi:10.1007/s11259-023-10224-9 .

Spasojević, Dragica, Zmejkoski, Danica, Milčić Matić, Natalija, Krnjaić, Dejan, Radotić, Ksenija, "Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study" in Veterinary Research Communications (2023),
https://doi.org/10.1007/s11259-023-10224-9 . .

TY  - CHAP
AU  - Radotić, Ksenija
AU  - Spasojević, Dragica
AU  - Zmejkoski, Danica
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12987
AB  - The material selected for biomedical application must be biocompatible, stable against physiological media, non-toxic, non-carcinogenic, corrosion-resistant, bio-inert, and stimuli-responsive, and have a low wear rate. Lignin is the most abundant aromatic polymer on Earth with a complex, recalcitrant structure. A lot of knowledge is acquired on its pre-treatments and processing for biomedical uses in the forms of hydrogels, films, composite materials, nanoparticles, and aerogels. To avoid the potential toxicity and to control the cytotoxicity of lignin-based materials, it is necessary to increase the purity of the starting source materials and understand their reactivity. The poor water solubility, broad molecular weight (MW) distribution, and highly complex structure of lignin restrict its wider clinical applications. These limitations may be effectively improved by chemical modifications or by using lignin fractions with a narrow MW range. Antioxidant and antimicrobial properties allow lignin to be used in pharmaceuticals, drug delivery, and wound healing. It is also reported as a good candidate for composite materials intended for bone regeneration scaffolds in tissue engineering. Future challenges for lignin-based materials for biomedical applications include achieving a better understanding of the structure, isolation, and batch properties of lignin, as well as structure–function relationships in its applications.
PB  - The Royal Society of Chemistry
T2  - Lignin-based Materials: Health Care and Medical Applications
T1  - Lignin-based material for biomedical applications: Basic requirements and properties
SP  - 85
EP  - 105
DO  - 10.1039/BK9781839167843-00085
ER  - 

@inbook{
author = "Radotić, Ksenija and Spasojević, Dragica and Zmejkoski, Danica",
year = "2023",
abstract = "The material selected for biomedical application must be biocompatible, stable against physiological media, non-toxic, non-carcinogenic, corrosion-resistant, bio-inert, and stimuli-responsive, and have a low wear rate. Lignin is the most abundant aromatic polymer on Earth with a complex, recalcitrant structure. A lot of knowledge is acquired on its pre-treatments and processing for biomedical uses in the forms of hydrogels, films, composite materials, nanoparticles, and aerogels. To avoid the potential toxicity and to control the cytotoxicity of lignin-based materials, it is necessary to increase the purity of the starting source materials and understand their reactivity. The poor water solubility, broad molecular weight (MW) distribution, and highly complex structure of lignin restrict its wider clinical applications. These limitations may be effectively improved by chemical modifications or by using lignin fractions with a narrow MW range. Antioxidant and antimicrobial properties allow lignin to be used in pharmaceuticals, drug delivery, and wound healing. It is also reported as a good candidate for composite materials intended for bone regeneration scaffolds in tissue engineering. Future challenges for lignin-based materials for biomedical applications include achieving a better understanding of the structure, isolation, and batch properties of lignin, as well as structure–function relationships in its applications.",
publisher = "The Royal Society of Chemistry",
journal = "Lignin-based Materials: Health Care and Medical Applications",
booktitle = "Lignin-based material for biomedical applications: Basic requirements and properties",
pages = "85-105",
doi = "10.1039/BK9781839167843-00085"
}

Radotić, K., Spasojević, D.,& Zmejkoski, D.. (2023). Lignin-based material for biomedical applications: Basic requirements and properties. in Lignin-based Materials: Health Care and Medical Applications
The Royal Society of Chemistry., 85-105.
https://doi.org/10.1039/BK9781839167843-00085

Radotić K, Spasojević D, Zmejkoski D. Lignin-based material for biomedical applications: Basic requirements and properties. in Lignin-based Materials: Health Care and Medical Applications. 2023;:85-105.
doi:10.1039/BK9781839167843-00085 .

Radotić, Ksenija, Spasojević, Dragica, Zmejkoski, Danica, "Lignin-based material for biomedical applications: Basic requirements and properties" in Lignin-based Materials: Health Care and Medical Applications (2023):85-105,
https://doi.org/10.1039/BK9781839167843-00085 . .

TY  - JOUR
AU  - Jovanović, Svetlana P.
AU  - Bonasera, Aurelio
AU  - Dorontić, Slađana
AU  - Zmejkoski, Danica
AU  - Milivojević, Dušan
AU  - Janakiev, Tamara
AU  - Todorović-Marković, Biljana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10633
AB  - Due to the increasing number of bacterial infections and the development of resistivity toward antibiotics, new materials and approaches for treatments must be urgently developed. The production of new materials should be ecologically friendly considering overall pollution with chemicals and economically acceptable and accessible to the wide population. Thus, the possibility of using biocompatible graphene quantum dots (GQDs) as an agent in photodynamic therapy was studied. First, dots were obtained using electrochemical cutting of graphite. In only one synthetic step using gamma irradiation, GQDs were doped with N atoms without any reagent. Obtained dots showed blue photoluminescence, with a diameter of 19–89 nm and optical band gap of 3.23–4.73 eV, featuring oxygen-containing, amino, and amide functional groups. Dots showed antioxidative activity; they quenched •OH at a concentration of 10 μg·mL−1, scavenged DPPH• radicals even at 5 μg·mL−1, and caused discoloration of KMnO4 at 30 μg·mL−1. Under light irradiation, dots were able to produce singlet oxygen, which remained stable for 10 min. Photoinduced effects by GQDs were studied on several bacterial strains (Listeria monocytogenes, Bacillus cereus, clinical strains of Streptococcus mutans, S. pyogenes, and S. sangunis, Pseudomonas aeruginosa, and one yeast strain Candida albicans) but antibacterial effects were not noticed.
T2  - Materials
T1  - Antioxidative and Photo-Induced Effects of Different Types of N-Doped Graphene Quantum Dots
VL  - 15
IS  - 19
SP  - 6525
DO  - 10.3390/ma15196525
ER  - 

@article{
author = "Jovanović, Svetlana P. and Bonasera, Aurelio and Dorontić, Slađana and Zmejkoski, Danica and Milivojević, Dušan and Janakiev, Tamara and Todorović-Marković, Biljana",
year = "2022",
abstract = "Due to the increasing number of bacterial infections and the development of resistivity toward antibiotics, new materials and approaches for treatments must be urgently developed. The production of new materials should be ecologically friendly considering overall pollution with chemicals and economically acceptable and accessible to the wide population. Thus, the possibility of using biocompatible graphene quantum dots (GQDs) as an agent in photodynamic therapy was studied. First, dots were obtained using electrochemical cutting of graphite. In only one synthetic step using gamma irradiation, GQDs were doped with N atoms without any reagent. Obtained dots showed blue photoluminescence, with a diameter of 19–89 nm and optical band gap of 3.23–4.73 eV, featuring oxygen-containing, amino, and amide functional groups. Dots showed antioxidative activity; they quenched •OH at a concentration of 10 μg·mL−1, scavenged DPPH• radicals even at 5 μg·mL−1, and caused discoloration of KMnO4 at 30 μg·mL−1. Under light irradiation, dots were able to produce singlet oxygen, which remained stable for 10 min. Photoinduced effects by GQDs were studied on several bacterial strains (Listeria monocytogenes, Bacillus cereus, clinical strains of Streptococcus mutans, S. pyogenes, and S. sangunis, Pseudomonas aeruginosa, and one yeast strain Candida albicans) but antibacterial effects were not noticed.",
journal = "Materials",
title = "Antioxidative and Photo-Induced Effects of Different Types of N-Doped Graphene Quantum Dots",
volume = "15",
number = "19",
pages = "6525",
doi = "10.3390/ma15196525"
}

Jovanović, S. P., Bonasera, A., Dorontić, S., Zmejkoski, D., Milivojević, D., Janakiev, T.,& Todorović-Marković, B.. (2022). Antioxidative and Photo-Induced Effects of Different Types of N-Doped Graphene Quantum Dots. in Materials, 15(19), 6525.
https://doi.org/10.3390/ma15196525

Jovanović SP, Bonasera A, Dorontić S, Zmejkoski D, Milivojević D, Janakiev T, Todorović-Marković B. Antioxidative and Photo-Induced Effects of Different Types of N-Doped Graphene Quantum Dots. in Materials. 2022;15(19):6525.
doi:10.3390/ma15196525 .

Jovanović, Svetlana P., Bonasera, Aurelio, Dorontić, Slađana, Zmejkoski, Danica, Milivojević, Dušan, Janakiev, Tamara, Todorović-Marković, Biljana, "Antioxidative and Photo-Induced Effects of Different Types of N-Doped Graphene Quantum Dots" in Materials, 15, no. 19 (2022):6525,
https://doi.org/10.3390/ma15196525 . .

TY  - JOUR
AU  - Marković, Zoran M.
AU  - Zmejkoski, Danica
AU  - Budimir, Milica
AU  - Bugarova, Nikol
AU  - Kleinova, Angela
AU  - Kuzman, Sanja
AU  - Špitalsky, Zdeno
AU  - Pavlović, Vladimir B.
AU  - Milivojević, Dušan
AU  - Todorović-Marković, Biljana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10634
AB  - Due to their unique structural properties bacterial cellulose (BC) hydrogels find possible usage in many fields such as cosmetology, food industry, or medicine. In this study, photoactive BC hydrogels are investigated through modifications of their structural, mechanical, and pro-oxidant properties resulting from graphene quantum dots (GQDs) encapsulation. Detailed structural analysis is conducted by atomic force microscopy, transmission electron microscopy and scanning electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction method. Dynamic mechanical analysis is performed to study the changes in storage modulus, loss modulus and tan δ. Pro-oxidative properties of new designed composites are tested by electron paramagnetic resonance (EPR). Structural and mechanical analyses show successful encapsulation of GQDs into BC whereas EPR measurements indicate high potential of these composites for singlet oxygen production.
T2  - Journal of Applied Polymer Science
T1  - Photoactive graphene quantum dots/bacterial cellulose hydrogels: Structural, mechanical, and pro-oxidant study
VL  - 139
IS  - 16
SP  - 51996
DO  - 10.1002/app.51996
ER  - 

@article{
author = "Marković, Zoran M. and Zmejkoski, Danica and Budimir, Milica and Bugarova, Nikol and Kleinova, Angela and Kuzman, Sanja and Špitalsky, Zdeno and Pavlović, Vladimir B. and Milivojević, Dušan and Todorović-Marković, Biljana",
year = "2022",
abstract = "Due to their unique structural properties bacterial cellulose (BC) hydrogels find possible usage in many fields such as cosmetology, food industry, or medicine. In this study, photoactive BC hydrogels are investigated through modifications of their structural, mechanical, and pro-oxidant properties resulting from graphene quantum dots (GQDs) encapsulation. Detailed structural analysis is conducted by atomic force microscopy, transmission electron microscopy and scanning electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction method. Dynamic mechanical analysis is performed to study the changes in storage modulus, loss modulus and tan δ. Pro-oxidative properties of new designed composites are tested by electron paramagnetic resonance (EPR). Structural and mechanical analyses show successful encapsulation of GQDs into BC whereas EPR measurements indicate high potential of these composites for singlet oxygen production.",
journal = "Journal of Applied Polymer Science",
title = "Photoactive graphene quantum dots/bacterial cellulose hydrogels: Structural, mechanical, and pro-oxidant study",
volume = "139",
number = "16",
pages = "51996",
doi = "10.1002/app.51996"
}

Marković, Z. M., Zmejkoski, D., Budimir, M., Bugarova, N., Kleinova, A., Kuzman, S., Špitalsky, Z., Pavlović, V. B., Milivojević, D.,& Todorović-Marković, B.. (2022). Photoactive graphene quantum dots/bacterial cellulose hydrogels: Structural, mechanical, and pro-oxidant study. in Journal of Applied Polymer Science, 139(16), 51996.
https://doi.org/10.1002/app.51996

Marković ZM, Zmejkoski D, Budimir M, Bugarova N, Kleinova A, Kuzman S, Špitalsky Z, Pavlović VB, Milivojević D, Todorović-Marković B. Photoactive graphene quantum dots/bacterial cellulose hydrogels: Structural, mechanical, and pro-oxidant study. in Journal of Applied Polymer Science. 2022;139(16):51996.
doi:10.1002/app.51996 .

Marković, Zoran M., Zmejkoski, Danica, Budimir, Milica, Bugarova, Nikol, Kleinova, Angela, Kuzman, Sanja, Špitalsky, Zdeno, Pavlović, Vladimir B., Milivojević, Dušan, Todorović-Marković, Biljana, "Photoactive graphene quantum dots/bacterial cellulose hydrogels: Structural, mechanical, and pro-oxidant study" in Journal of Applied Polymer Science, 139, no. 16 (2022):51996,
https://doi.org/10.1002/app.51996 . .

TY  - JOUR
AU  - Zmejkoski, Danica
AU  - Zdravković, Nemanja M.
AU  - Trišić, Dijana D.
AU  - Budimir, Milica
AU  - Marković, Zoran M.
AU  - Kozyrovska, Natalia O.
AU  - Todorović-Marković, Biljana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9957
AB  - Since the pathogenic bacteria biofilms are involved in 70% of chronic infections and their resistance to antibiotics is increased, the research in this field requires new healing agents. New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) – BC-Chi and chitosan nanoparticles (nChiD) – BC-nChiD. nChiD were obtained by gamma irradiation at doses: 20, 40 and 60 kGy. Physical and chemical analyses showed incorporation of Chi and encapsulation of nChiD into BC. The BC-Chi has the highest average surface roughness. BC-nChiD hydrogels show an irradiated dose-dependent increase of average surface roughness. New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height. The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing). All obtained results confirmed both composite hydrogels as promising chronic wound healing agents.
T2  - International Journal of Biological Macromolecules
T1  - Chronic wound dressings – Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels
VL  - 191
SP  - 315
EP  - 323
DO  - 10.1016/j.ijbiomac.2021.09.118
ER  - 

@article{
author = "Zmejkoski, Danica and Zdravković, Nemanja M. and Trišić, Dijana D. and Budimir, Milica and Marković, Zoran M. and Kozyrovska, Natalia O. and Todorović-Marković, Biljana",
year = "2021",
abstract = "Since the pathogenic bacteria biofilms are involved in 70% of chronic infections and their resistance to antibiotics is increased, the research in this field requires new healing agents. New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) – BC-Chi and chitosan nanoparticles (nChiD) – BC-nChiD. nChiD were obtained by gamma irradiation at doses: 20, 40 and 60 kGy. Physical and chemical analyses showed incorporation of Chi and encapsulation of nChiD into BC. The BC-Chi has the highest average surface roughness. BC-nChiD hydrogels show an irradiated dose-dependent increase of average surface roughness. New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height. The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing). All obtained results confirmed both composite hydrogels as promising chronic wound healing agents.",
journal = "International Journal of Biological Macromolecules",
title = "Chronic wound dressings – Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels",
volume = "191",
pages = "315-323",
doi = "10.1016/j.ijbiomac.2021.09.118"
}

Zmejkoski, D., Zdravković, N. M., Trišić, D. D., Budimir, M., Marković, Z. M., Kozyrovska, N. O.,& Todorović-Marković, B.. (2021). Chronic wound dressings – Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels. in International Journal of Biological Macromolecules, 191, 315-323.
https://doi.org/10.1016/j.ijbiomac.2021.09.118

Zmejkoski D, Zdravković NM, Trišić DD, Budimir M, Marković ZM, Kozyrovska NO, Todorović-Marković B. Chronic wound dressings – Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels. in International Journal of Biological Macromolecules. 2021;191:315-323.
doi:10.1016/j.ijbiomac.2021.09.118 .

Zmejkoski, Danica, Zdravković, Nemanja M., Trišić, Dijana D., Budimir, Milica, Marković, Zoran M., Kozyrovska, Natalia O., Todorović-Marković, Biljana, "Chronic wound dressings – Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels" in International Journal of Biological Macromolecules, 191 (2021):315-323,
https://doi.org/10.1016/j.ijbiomac.2021.09.118 . .

TY  - JOUR
AU  - Zmejkoski, Danica
AU  - Marković, Zoran M.
AU  - Zdravković, Nemanja M.
AU  - Trišić, Dijana
AU  - Budimir, Milica
AU  - Kuzman, Sanja
AU  - Kozyrovska, Natalia O.
AU  - Orlovska, Iryna V.
AU  - Bugárová, Nikol
AU  - Petrović, Đorđe
AU  - Kováčová, Mária
AU  - Kleinová, Angela
AU  - Špitalský, Zdeno
AU  - Pavlović, Vladimir B.
AU  - Todorović-Marković, Biljana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9143
AB  - Therapy of bacterial urinary tract infections (UTIs) and catheter associated urinary tract infections (CAUTIs) is still a great challenge because of the resistance of bacteria to nowadays used antibiotics and encrustation of catheters. Bacterial cellulose (BC) as a biocompatible material with a high porosity allows incorporation of different materials in its three dimensional network structure. In this work a low molecular weight chitosan (Chi) polymer is incorporated in BC with different concentrations. Different characterization techniques are used to investigate structural and optical properties of these composites. Radical scavenging activity test shows moderate antioxidant activity of these biocompatible composites whereasin vitrorelease test shows that 13.3% of chitosan is released after 72 h. Antibacterial testing of BC-Chi composites conducted on Gram-positive and Gram-negative bacteria causing UTIs and CAUTIs (Escherichia coli,Pseudomonas aeruginosa,Klebsiella pneumoniae) and encrustation (Proteus mirabilis) show bactericidal effect. The morphology analysis of bacteria after the application of BC-Chi shows that they are flattened with a rough surface, with a tendency to agglomerate and with decreased length and width. All obtained results show that BC-Chi composites might be considered as potential biomedical agents in treatment of UTIs and CAUTIs and as a urinary catheter coating in encrustation prevention.
T2  - RSC Advances
T1  - Bactericidal and antioxidant bacterial cellulose hydrogels doped with chitosan as potential urinary tract infection biomedical agent
VL  - 11
IS  - 15
SP  - 8559
EP  - 8568
DO  - 10.1039/D0RA10782D
ER  - 

@article{
author = "Zmejkoski, Danica and Marković, Zoran M. and Zdravković, Nemanja M. and Trišić, Dijana and Budimir, Milica and Kuzman, Sanja and Kozyrovska, Natalia O. and Orlovska, Iryna V. and Bugárová, Nikol and Petrović, Đorđe and Kováčová, Mária and Kleinová, Angela and Špitalský, Zdeno and Pavlović, Vladimir B. and Todorović-Marković, Biljana",
year = "2021",
abstract = "Therapy of bacterial urinary tract infections (UTIs) and catheter associated urinary tract infections (CAUTIs) is still a great challenge because of the resistance of bacteria to nowadays used antibiotics and encrustation of catheters. Bacterial cellulose (BC) as a biocompatible material with a high porosity allows incorporation of different materials in its three dimensional network structure. In this work a low molecular weight chitosan (Chi) polymer is incorporated in BC with different concentrations. Different characterization techniques are used to investigate structural and optical properties of these composites. Radical scavenging activity test shows moderate antioxidant activity of these biocompatible composites whereasin vitrorelease test shows that 13.3% of chitosan is released after 72 h. Antibacterial testing of BC-Chi composites conducted on Gram-positive and Gram-negative bacteria causing UTIs and CAUTIs (Escherichia coli,Pseudomonas aeruginosa,Klebsiella pneumoniae) and encrustation (Proteus mirabilis) show bactericidal effect. The morphology analysis of bacteria after the application of BC-Chi shows that they are flattened with a rough surface, with a tendency to agglomerate and with decreased length and width. All obtained results show that BC-Chi composites might be considered as potential biomedical agents in treatment of UTIs and CAUTIs and as a urinary catheter coating in encrustation prevention.",
journal = "RSC Advances",
title = "Bactericidal and antioxidant bacterial cellulose hydrogels doped with chitosan as potential urinary tract infection biomedical agent",
volume = "11",
number = "15",
pages = "8559-8568",
doi = "10.1039/D0RA10782D"
}

Zmejkoski, D., Marković, Z. M., Zdravković, N. M., Trišić, D., Budimir, M., Kuzman, S., Kozyrovska, N. O., Orlovska, I. V., Bugárová, N., Petrović, Đ., Kováčová, M., Kleinová, A., Špitalský, Z., Pavlović, V. B.,& Todorović-Marković, B.. (2021). Bactericidal and antioxidant bacterial cellulose hydrogels doped with chitosan as potential urinary tract infection biomedical agent. in RSC Advances, 11(15), 8559-8568.
https://doi.org/10.1039/D0RA10782D

Zmejkoski D, Marković ZM, Zdravković NM, Trišić D, Budimir M, Kuzman S, Kozyrovska NO, Orlovska IV, Bugárová N, Petrović Đ, Kováčová M, Kleinová A, Špitalský Z, Pavlović VB, Todorović-Marković B. Bactericidal and antioxidant bacterial cellulose hydrogels doped with chitosan as potential urinary tract infection biomedical agent. in RSC Advances. 2021;11(15):8559-8568.
doi:10.1039/D0RA10782D .

Zmejkoski, Danica, Marković, Zoran M., Zdravković, Nemanja M., Trišić, Dijana, Budimir, Milica, Kuzman, Sanja, Kozyrovska, Natalia O., Orlovska, Iryna V., Bugárová, Nikol, Petrović, Đorđe, Kováčová, Mária, Kleinová, Angela, Špitalský, Zdeno, Pavlović, Vladimir B., Todorović-Marković, Biljana, "Bactericidal and antioxidant bacterial cellulose hydrogels doped with chitosan as potential urinary tract infection biomedical agent" in RSC Advances, 11, no. 15 (2021):8559-8568,
https://doi.org/10.1039/D0RA10782D . .

TY  - JOUR
AU  - Zmejkoski, Danica
AU  - Marković, Zoran M.
AU  - Budimir, Milica
AU  - Zdravković, Nemanja M.
AU  - Trišić, Dijana 
AU  - Bugárová, Nikol
AU  - Danko, Martin
AU  - Kozyrovska, Natalia O.
AU  - Špitalský, Zdeno
AU  - Kleinová, Angela
AU  - Kuzman, Sanja
AU  - Pavlović, Vladimir B.
AU  - Todorović-Marković, Biljana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9566
AB  - Bacterial infection and their resistance to known antibiotics delays wound healing. In this study, nanochitosan dots (nChiD) produced by gamma irradiation have been encapsulated in bacterial cellulose (BC) polymer matrix to study the antibacterial potentials of these nanocomposites and their possible usage in wound healing treatment (scratch assay). Detailed analyses show that nChiDs have disc-like shape and average diameter in the range of 40 to 60 nm depending of the applied dose. All nChiDs as well as BC-nChiD nanocomposites emit green photoluminescence independently on the excitation wavelengths. The new designed nanocomposites do not have a cytotoxic effect; antioxidant analysis shows their moderate radical scavenging activity whereas antibacterial properties show significant growth inhibition of strains mostly found in difficult-to-heal wounds. The obtained results confirm that new designed BC-nChiD nanocomposites might be potential agent in wound healing treatment. © 2021 Elsevier B.V.
T2  - Materials Science and Engineering: C
T1  - Photoactive and antioxidant nanochitosan dots/biocellulose hydrogels for wound healing treatment
VL  - 122
SP  - 111925
DO  - 10.1016/j.msec.2021.111925
ER  - 

@article{
author = "Zmejkoski, Danica and Marković, Zoran M. and Budimir, Milica and Zdravković, Nemanja M. and Trišić, Dijana  and Bugárová, Nikol and Danko, Martin and Kozyrovska, Natalia O. and Špitalský, Zdeno and Kleinová, Angela and Kuzman, Sanja and Pavlović, Vladimir B. and Todorović-Marković, Biljana",
year = "2021",
abstract = "Bacterial infection and their resistance to known antibiotics delays wound healing. In this study, nanochitosan dots (nChiD) produced by gamma irradiation have been encapsulated in bacterial cellulose (BC) polymer matrix to study the antibacterial potentials of these nanocomposites and their possible usage in wound healing treatment (scratch assay). Detailed analyses show that nChiDs have disc-like shape and average diameter in the range of 40 to 60 nm depending of the applied dose. All nChiDs as well as BC-nChiD nanocomposites emit green photoluminescence independently on the excitation wavelengths. The new designed nanocomposites do not have a cytotoxic effect; antioxidant analysis shows their moderate radical scavenging activity whereas antibacterial properties show significant growth inhibition of strains mostly found in difficult-to-heal wounds. The obtained results confirm that new designed BC-nChiD nanocomposites might be potential agent in wound healing treatment. © 2021 Elsevier B.V.",
journal = "Materials Science and Engineering: C",
title = "Photoactive and antioxidant nanochitosan dots/biocellulose hydrogels for wound healing treatment",
volume = "122",
pages = "111925",
doi = "10.1016/j.msec.2021.111925"
}

Zmejkoski, D., Marković, Z. M., Budimir, M., Zdravković, N. M., Trišić, D., Bugárová, N., Danko, M., Kozyrovska, N. O., Špitalský, Z., Kleinová, A., Kuzman, S., Pavlović, V. B.,& Todorović-Marković, B.. (2021). Photoactive and antioxidant nanochitosan dots/biocellulose hydrogels for wound healing treatment. in Materials Science and Engineering: C, 122, 111925.
https://doi.org/10.1016/j.msec.2021.111925

Zmejkoski D, Marković ZM, Budimir M, Zdravković NM, Trišić D, Bugárová N, Danko M, Kozyrovska NO, Špitalský Z, Kleinová A, Kuzman S, Pavlović VB, Todorović-Marković B. Photoactive and antioxidant nanochitosan dots/biocellulose hydrogels for wound healing treatment. in Materials Science and Engineering: C. 2021;122:111925.
doi:10.1016/j.msec.2021.111925 .

Zmejkoski, Danica, Marković, Zoran M., Budimir, Milica, Zdravković, Nemanja M., Trišić, Dijana , Bugárová, Nikol, Danko, Martin, Kozyrovska, Natalia O., Špitalský, Zdeno, Kleinová, Angela, Kuzman, Sanja, Pavlović, Vladimir B., Todorović-Marković, Biljana, "Photoactive and antioxidant nanochitosan dots/biocellulose hydrogels for wound healing treatment" in Materials Science and Engineering: C, 122 (2021):111925,
https://doi.org/10.1016/j.msec.2021.111925 . .

TY  - JOUR
AU  - Orlovska, Iryna
AU  - Podolich, Olga
AU  - Kukharenko, Olga
AU  - Zaets, Iryna
AU  - Reva, Oleg
AU  - Khirunenko, Ludmila
AU  - Zmejkoski, Danica
AU  - Rogalsky, Sergiy
AU  - Barh, Debmalya
AU  - Tiwari, Sandeep
AU  - Kumavath, Ranjith
AU  - Góes-Neto, Aristóteles
AU  - Azevedo, Vasco
AU  - Brenig, Bertram
AU  - Ghosh, Preetam
AU  - de Vera, Jean-Pierre
AU  - Kozyrovska, Natalia
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9849
AB  - Cellulose is a widespread macromolecule in terrestrial environments and a major architectural component of microbial biofilm. Therefore, cellulose might be considered a biosignature that indicates the presence of microbial life. We present, for the first time, characteristics of bacterial cellulose after long-term spaceflight and exposure to simuled Mars-like stressors. The pristine cellulose-based pellicle membranes from a kombucha microbial community (KMC) were exposed outside the International Space Station, and after their return to Earth, the samples were reactivated and cultured for 2.5 years to discern whether the KMC could be restored. Analyses of cellulose polymer integrity and mechanical properties of cellulose-based pellicle films, as well as the cellulose biosynthesis-related genes' structure and expression, were performed. We observed that (i) the cellulose polymer integrity was not significantly changed under Mars-like conditions; (ii) de novo cellulose production was 1.5 times decreased in exposed KMC samples; (iii) the dry cellulose yield from the reisolated Komagataeibacter oboediens was 1.7 times lower than by wild type; (iv) there was no significant change in mechanical properties of the de novo synthesized cellulose-based pellicles produced by the exposed KMCs and K. oboediens; and (v) the gene, encoding biosynthesis of cellulose (bcsA) of the K. oboediens, was downregulated, and no topological change or mutation was observed in any of the bcs operon genes, indicating that the decreased cellulose production by the space-exposed samples was probably due to epigenetic regulation. Our results suggest that the cellulose-based pellicle could be a good material with which to protect microbial communities during space journeys, and the cellulose produced by KMC members could be suitable in the fabrication of consumer goods for extraterrestrial locations.
T2  - Astrobiology
T1  - Bacterial Cellulose Retains Robustness but Its Synthesis Declines After Exposure to a Mars-like Environment Simulated Outside the International Space Station
VL  - 21
IS  - 6
SP  - 706
EP  - 717
DO  - 10.1089/ast.2020.2332
ER  - 

@article{
author = "Orlovska, Iryna and Podolich, Olga and Kukharenko, Olga and Zaets, Iryna and Reva, Oleg and Khirunenko, Ludmila and Zmejkoski, Danica and Rogalsky, Sergiy and Barh, Debmalya and Tiwari, Sandeep and Kumavath, Ranjith and Góes-Neto, Aristóteles and Azevedo, Vasco and Brenig, Bertram and Ghosh, Preetam and de Vera, Jean-Pierre and Kozyrovska, Natalia",
year = "2021",
abstract = "Cellulose is a widespread macromolecule in terrestrial environments and a major architectural component of microbial biofilm. Therefore, cellulose might be considered a biosignature that indicates the presence of microbial life. We present, for the first time, characteristics of bacterial cellulose after long-term spaceflight and exposure to simuled Mars-like stressors. The pristine cellulose-based pellicle membranes from a kombucha microbial community (KMC) were exposed outside the International Space Station, and after their return to Earth, the samples were reactivated and cultured for 2.5 years to discern whether the KMC could be restored. Analyses of cellulose polymer integrity and mechanical properties of cellulose-based pellicle films, as well as the cellulose biosynthesis-related genes' structure and expression, were performed. We observed that (i) the cellulose polymer integrity was not significantly changed under Mars-like conditions; (ii) de novo cellulose production was 1.5 times decreased in exposed KMC samples; (iii) the dry cellulose yield from the reisolated Komagataeibacter oboediens was 1.7 times lower than by wild type; (iv) there was no significant change in mechanical properties of the de novo synthesized cellulose-based pellicles produced by the exposed KMCs and K. oboediens; and (v) the gene, encoding biosynthesis of cellulose (bcsA) of the K. oboediens, was downregulated, and no topological change or mutation was observed in any of the bcs operon genes, indicating that the decreased cellulose production by the space-exposed samples was probably due to epigenetic regulation. Our results suggest that the cellulose-based pellicle could be a good material with which to protect microbial communities during space journeys, and the cellulose produced by KMC members could be suitable in the fabrication of consumer goods for extraterrestrial locations.",
journal = "Astrobiology",
title = "Bacterial Cellulose Retains Robustness but Its Synthesis Declines After Exposure to a Mars-like Environment Simulated Outside the International Space Station",
volume = "21",
number = "6",
pages = "706-717",
doi = "10.1089/ast.2020.2332"
}

Orlovska, I., Podolich, O., Kukharenko, O., Zaets, I., Reva, O., Khirunenko, L., Zmejkoski, D., Rogalsky, S., Barh, D., Tiwari, S., Kumavath, R., Góes-Neto, A., Azevedo, V., Brenig, B., Ghosh, P., de Vera, J.,& Kozyrovska, N.. (2021). Bacterial Cellulose Retains Robustness but Its Synthesis Declines After Exposure to a Mars-like Environment Simulated Outside the International Space Station. in Astrobiology, 21(6), 706-717.
https://doi.org/10.1089/ast.2020.2332

Orlovska I, Podolich O, Kukharenko O, Zaets I, Reva O, Khirunenko L, Zmejkoski D, Rogalsky S, Barh D, Tiwari S, Kumavath R, Góes-Neto A, Azevedo V, Brenig B, Ghosh P, de Vera J, Kozyrovska N. Bacterial Cellulose Retains Robustness but Its Synthesis Declines After Exposure to a Mars-like Environment Simulated Outside the International Space Station. in Astrobiology. 2021;21(6):706-717.
doi:10.1089/ast.2020.2332 .

Orlovska, Iryna, Podolich, Olga, Kukharenko, Olga, Zaets, Iryna, Reva, Oleg, Khirunenko, Ludmila, Zmejkoski, Danica, Rogalsky, Sergiy, Barh, Debmalya, Tiwari, Sandeep, Kumavath, Ranjith, Góes-Neto, Aristóteles, Azevedo, Vasco, Brenig, Bertram, Ghosh, Preetam, de Vera, Jean-Pierre, Kozyrovska, Natalia, "Bacterial Cellulose Retains Robustness but Its Synthesis Declines After Exposure to a Mars-like Environment Simulated Outside the International Space Station" in Astrobiology, 21, no. 6 (2021):706-717,
https://doi.org/10.1089/ast.2020.2332 . .

TY  - JOUR
AU  - Zmejkoski, Danica
AU  - Spasojević, Dragica
AU  - Orlovska, Irina V.
AU  - Kozyrovska, Natalia O.
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Dmitrović, Svetlana
AU  - Matović, Branko
AU  - Tasić, Nikola B.
AU  - Maksimović, Vuk M.
AU  - Sosnin, Mikhail
AU  - Radotić, Ksenija
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7769
AB  - Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC) and dehydrogenative polymer of coniferyl alcohol (DHP), BC-DHP, was designed. Novel composite showed inhibitory or bactericidal effects against selected pathogenic bacteria, including clinically isolated ones. The highest release rate of DHP was in the first hour, while after 24 h there was still slow release of small amounts of DHP from BC-DHP during 72 h monitoring. High-performance liquid chromatography coupled with mass-spectrometry showed that BC-DHP releases DHP oligomers, which are proposed to be antimicrobially active DHP fractions. Scanning electron microscopy and atomic force microscopy micrographs proved a dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number and size in the cellulose membrane. The Fourier-transform infrared absorption spectra of the BC-DHP showed that DHP was partly bound to the BC matrix. The swelling and crystallinity degree were dose-dependent. All obtained results confirmed BC-DHP composite as a promising hydrogel for wounds healing.
T2  - International Journal of Biological Macromolecules
T1  - Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing
VL  - 118
SP  - 494
EP  - 503
DO  - 10.1016/j.ijbiomac.2018.06.067
ER  - 

@article{
author = "Zmejkoski, Danica and Spasojević, Dragica and Orlovska, Irina V. and Kozyrovska, Natalia O. and Soković, Marina and Glamočlija, Jasmina and Dmitrović, Svetlana and Matović, Branko and Tasić, Nikola B. and Maksimović, Vuk M. and Sosnin, Mikhail and Radotić, Ksenija",
year = "2018",
abstract = "Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC) and dehydrogenative polymer of coniferyl alcohol (DHP), BC-DHP, was designed. Novel composite showed inhibitory or bactericidal effects against selected pathogenic bacteria, including clinically isolated ones. The highest release rate of DHP was in the first hour, while after 24 h there was still slow release of small amounts of DHP from BC-DHP during 72 h monitoring. High-performance liquid chromatography coupled with mass-spectrometry showed that BC-DHP releases DHP oligomers, which are proposed to be antimicrobially active DHP fractions. Scanning electron microscopy and atomic force microscopy micrographs proved a dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number and size in the cellulose membrane. The Fourier-transform infrared absorption spectra of the BC-DHP showed that DHP was partly bound to the BC matrix. The swelling and crystallinity degree were dose-dependent. All obtained results confirmed BC-DHP composite as a promising hydrogel for wounds healing.",
journal = "International Journal of Biological Macromolecules",
title = "Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing",
volume = "118",
pages = "494-503",
doi = "10.1016/j.ijbiomac.2018.06.067"
}

Zmejkoski, D., Spasojević, D., Orlovska, I. V., Kozyrovska, N. O., Soković, M., Glamočlija, J., Dmitrović, S., Matović, B., Tasić, N. B., Maksimović, V. M., Sosnin, M.,& Radotić, K.. (2018). Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing. in International Journal of Biological Macromolecules, 118, 494-503.
https://doi.org/10.1016/j.ijbiomac.2018.06.067

Zmejkoski D, Spasojević D, Orlovska IV, Kozyrovska NO, Soković M, Glamočlija J, Dmitrović S, Matović B, Tasić NB, Maksimović VM, Sosnin M, Radotić K. Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing. in International Journal of Biological Macromolecules. 2018;118:494-503.
doi:10.1016/j.ijbiomac.2018.06.067 .

Zmejkoski, Danica, Spasojević, Dragica, Orlovska, Irina V., Kozyrovska, Natalia O., Soković, Marina, Glamočlija, Jasmina, Dmitrović, Svetlana, Matović, Branko, Tasić, Nikola B., Maksimović, Vuk M., Sosnin, Mikhail, Radotić, Ksenija, "Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing" in International Journal of Biological Macromolecules, 118 (2018):494-503,
https://doi.org/10.1016/j.ijbiomac.2018.06.067 . .

TY  - JOUR
AU  - Orlovska, Irina V.
AU  - Yakovenko, I. O.
AU  - Haidak, Andriy H.
AU  - Zmejkoski, Danica
AU  - Kozyrovska, Natalia O.
PY  - 2018
UR  - http://biopolymers.org.ua/doi/10.7124/bc.000979
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8029
AB  - Regenerative medicine therapy is inspired by current research advances in cellular biology, genetic engineering, synthetic biology, material sciences and so far contributes to the traditional therapy, making resistant diseases curable. Nowadays, chronic wound healing is possible due to cell-based regenerative technologies and recent non-cell therapeutic approaches. Here we review clinical applications of human stem cells, as well as cellular and tissue products as alternatives to the traditional therapy of non-healing wounds. The cell-based technologies for tissue regeneration and bioengineering utilize stem cells that are either injected into bloodstream or positioned directly into the target area. Cell-free regeneration technologies require either stem cell products, i.e., secretomes or their separate components, extracellular membrane vesicles, or tissue products. The stem cell therapies are designed to replace critically absent components of wounded or degenerative tissue. The stem cell secretome can promote the repair of damaged tissues independently of parent cells. Extracellular membrane vesicles mimic and recapitulate the mechanisms of stem cells in tissue regeneration and therefore might be promising for chronic wound and severe burns healing. The tissue products traditionally remain efficient wound healing remedies along with emerging advanced technologies. © 2018 I. V. Orlovska et al.
T2  - Biopolymers and Cell
T1  - Regenerative therapy for the nonhealing cutaneous wounds
VL  - 34
IS  - 3
SP  - 171
EP  - 195
DO  - 10.7124/bc.000979
ER  - 

@article{
author = "Orlovska, Irina V. and Yakovenko, I. O. and Haidak, Andriy H. and Zmejkoski, Danica and Kozyrovska, Natalia O.",
year = "2018",
abstract = "Regenerative medicine therapy is inspired by current research advances in cellular biology, genetic engineering, synthetic biology, material sciences and so far contributes to the traditional therapy, making resistant diseases curable. Nowadays, chronic wound healing is possible due to cell-based regenerative technologies and recent non-cell therapeutic approaches. Here we review clinical applications of human stem cells, as well as cellular and tissue products as alternatives to the traditional therapy of non-healing wounds. The cell-based technologies for tissue regeneration and bioengineering utilize stem cells that are either injected into bloodstream or positioned directly into the target area. Cell-free regeneration technologies require either stem cell products, i.e., secretomes or their separate components, extracellular membrane vesicles, or tissue products. The stem cell therapies are designed to replace critically absent components of wounded or degenerative tissue. The stem cell secretome can promote the repair of damaged tissues independently of parent cells. Extracellular membrane vesicles mimic and recapitulate the mechanisms of stem cells in tissue regeneration and therefore might be promising for chronic wound and severe burns healing. The tissue products traditionally remain efficient wound healing remedies along with emerging advanced technologies. © 2018 I. V. Orlovska et al.",
journal = "Biopolymers and Cell",
title = "Regenerative therapy for the nonhealing cutaneous wounds",
volume = "34",
number = "3",
pages = "171-195",
doi = "10.7124/bc.000979"
}

Orlovska, I. V., Yakovenko, I. O., Haidak, A. H., Zmejkoski, D.,& Kozyrovska, N. O.. (2018). Regenerative therapy for the nonhealing cutaneous wounds. in Biopolymers and Cell, 34(3), 171-195.
https://doi.org/10.7124/bc.000979

Orlovska IV, Yakovenko IO, Haidak AH, Zmejkoski D, Kozyrovska NO. Regenerative therapy for the nonhealing cutaneous wounds. in Biopolymers and Cell. 2018;34(3):171-195.
doi:10.7124/bc.000979 .

Orlovska, Irina V., Yakovenko, I. O., Haidak, Andriy H., Zmejkoski, Danica, Kozyrovska, Natalia O., "Regenerative therapy for the nonhealing cutaneous wounds" in Biopolymers and Cell, 34, no. 3 (2018):171-195,
https://doi.org/10.7124/bc.000979 . .

TY  - CONF
AU  - Orlovska, Iryna
AU  - Zmejkoski, Danica
AU  - Khirunenko, L.
AU  - Kozyrovska, N.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13008
AB  - Biofilm-forming multi-drug resistant pathogens may account for 10% of all hospital acquired infections and can occur in burns, post-surgical wounds, pressure injuries and diabetic foot wounds. Hydrogels – natural polymers or composite materials – represent both a bandage and a matrix for therapeutics in a wound healing. Novel composite based on bacterial cellulose and synthetic analog of lignin - DHP (dehydrogenate polymer) has been designed with the aim to suppress pathogenic agents in incurable wounds. The FT-IR spectra of the BC/DHP membrane established an appearance of characteristic for DHP absorption bands in the composite in the 1600–1510 cm−1 spectral range. A clear position of these bands in BC/DHP showed that DHP had been incorporated into the BC polymeric matrix. In addition, disappearance of the bands at the 3630–3650 cm−1 spectral range assigned to the cellulose free OH vibration, strengthening the bands assigned to intermolecular interactions and an appearance of a new band at 3293 cm−1 could serve as a proof of the interaction of BC molecules and DHP. Micrographs obtained from a scanning electron microscopy proved the dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number in the cellulose membrane. The antimicrobial activity of composites BC/DHP were tested the in vitro experiments, using Pseudomonas aeruginosa (УМВ-100) (MTT-test) and showed that BC/DHP inhibited P. aeruginosa. BC/DHP composite will be promising hydrogel for a healing of infected incurable wounds.
C3  - Integrative Biology & Medicine : Program and Abstracts
T1  - A composite of bacterial cellulose and lignin for a healing of incurable wounds
SP  - 53
EP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13008
ER  - 

@conference{
author = "Orlovska, Iryna and Zmejkoski, Danica and Khirunenko, L. and Kozyrovska, N.",
year = "2017",
abstract = "Biofilm-forming multi-drug resistant pathogens may account for 10% of all hospital acquired infections and can occur in burns, post-surgical wounds, pressure injuries and diabetic foot wounds. Hydrogels – natural polymers or composite materials – represent both a bandage and a matrix for therapeutics in a wound healing. Novel composite based on bacterial cellulose and synthetic analog of lignin - DHP (dehydrogenate polymer) has been designed with the aim to suppress pathogenic agents in incurable wounds. The FT-IR spectra of the BC/DHP membrane established an appearance of characteristic for DHP absorption bands in the composite in the 1600–1510 cm−1 spectral range. A clear position of these bands in BC/DHP showed that DHP had been incorporated into the BC polymeric matrix. In addition, disappearance of the bands at the 3630–3650 cm−1 spectral range assigned to the cellulose free OH vibration, strengthening the bands assigned to intermolecular interactions and an appearance of a new band at 3293 cm−1 could serve as a proof of the interaction of BC molecules and DHP. Micrographs obtained from a scanning electron microscopy proved the dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number in the cellulose membrane. The antimicrobial activity of composites BC/DHP were tested the in vitro experiments, using Pseudomonas aeruginosa (УМВ-100) (MTT-test) and showed that BC/DHP inhibited P. aeruginosa. BC/DHP composite will be promising hydrogel for a healing of infected incurable wounds.",
journal = "Integrative Biology & Medicine : Program and Abstracts",
title = "A composite of bacterial cellulose and lignin for a healing of incurable wounds",
pages = "53-53",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13008"
}

Orlovska, I., Zmejkoski, D., Khirunenko, L.,& Kozyrovska, N.. (2017). A composite of bacterial cellulose and lignin for a healing of incurable wounds. in Integrative Biology & Medicine : Program and Abstracts, 53-53.
https://hdl.handle.net/21.15107/rcub_vinar_13008

Orlovska I, Zmejkoski D, Khirunenko L, Kozyrovska N. A composite of bacterial cellulose and lignin for a healing of incurable wounds. in Integrative Biology & Medicine : Program and Abstracts. 2017;:53-53.
https://hdl.handle.net/21.15107/rcub_vinar_13008 .

Orlovska, Iryna, Zmejkoski, Danica, Khirunenko, L., Kozyrovska, N., "A composite of bacterial cellulose and lignin for a healing of incurable wounds" in Integrative Biology & Medicine : Program and Abstracts (2017):53-53,
https://hdl.handle.net/21.15107/rcub_vinar_13008 .

TY  - JOUR
AU  - Zmejkoski, Danica
AU  - Petković, Branka
AU  - Pavković-Lučić, Sofija
AU  - Prolić, Zlatko M.
AU  - Anđelković, Marko
AU  - Savić, Tatjana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1540
AB  - Purpose: Extremely low frequency (ELF) magnetic fields as essential ecological factors may induce specific responses in genetically different lines. The object of this study was to investigate the impact of the ELF magnetic field on fitness components and locomotor activity of five Drosophila subobscura isofemale (IF) lines.Materials and methods: Each D. subobscura IF line, arbitrarily named: B16/1, B24/4, B39/1, B57/2 and B69/5, was maintained in five full-sib inbreeding generations. Their genetic structures were defined based on the mitochondrial DNA variability. Egg-first instar larvae and 1-day-old flies were exposed to an ELF magnetic field (50Hz, 0.5mT, 48h) and thereafter, fitness components and locomotor activity of males and females in an open field test were observed for each selected IF line, respectively.Results: Exposure of egg-first instar larvae to an ELF magnetic field shortened developmental time, and did not affect the viability and sex ratio of D. subobscura IF lines. Exposure of 1-day-old males and females IF lines B16/1 and B24/4 to an ELF magnetic field significantly decreased their locomotor activity and this effect lasted longer in females than males.Conclusions: These results indicate various responses of D. subobscura IF lines to the applied ELF magnetic field depending on their genetic background.
T2  - International Journal of Radiation Biology
T1  - Different responses of Drosophila subobscura isofemale lines to extremely low frequency magnetic field (50Hz, 0.5mT): fitness components and locomotor activity
VL  - 93
IS  - 5
SP  - 544
EP  - 552
DO  - 10.1080/09553002.2017.1268281
ER  - 

@article{
author = "Zmejkoski, Danica and Petković, Branka and Pavković-Lučić, Sofija and Prolić, Zlatko M. and Anđelković, Marko and Savić, Tatjana",
year = "2017",
abstract = "Purpose: Extremely low frequency (ELF) magnetic fields as essential ecological factors may induce specific responses in genetically different lines. The object of this study was to investigate the impact of the ELF magnetic field on fitness components and locomotor activity of five Drosophila subobscura isofemale (IF) lines.Materials and methods: Each D. subobscura IF line, arbitrarily named: B16/1, B24/4, B39/1, B57/2 and B69/5, was maintained in five full-sib inbreeding generations. Their genetic structures were defined based on the mitochondrial DNA variability. Egg-first instar larvae and 1-day-old flies were exposed to an ELF magnetic field (50Hz, 0.5mT, 48h) and thereafter, fitness components and locomotor activity of males and females in an open field test were observed for each selected IF line, respectively.Results: Exposure of egg-first instar larvae to an ELF magnetic field shortened developmental time, and did not affect the viability and sex ratio of D. subobscura IF lines. Exposure of 1-day-old males and females IF lines B16/1 and B24/4 to an ELF magnetic field significantly decreased their locomotor activity and this effect lasted longer in females than males.Conclusions: These results indicate various responses of D. subobscura IF lines to the applied ELF magnetic field depending on their genetic background.",
journal = "International Journal of Radiation Biology",
title = "Different responses of Drosophila subobscura isofemale lines to extremely low frequency magnetic field (50Hz, 0.5mT): fitness components and locomotor activity",
volume = "93",
number = "5",
pages = "544-552",
doi = "10.1080/09553002.2017.1268281"
}

Zmejkoski, D., Petković, B., Pavković-Lučić, S., Prolić, Z. M., Anđelković, M.,& Savić, T.. (2017). Different responses of Drosophila subobscura isofemale lines to extremely low frequency magnetic field (50Hz, 0.5mT): fitness components and locomotor activity. in International Journal of Radiation Biology, 93(5), 544-552.
https://doi.org/10.1080/09553002.2017.1268281

Zmejkoski D, Petković B, Pavković-Lučić S, Prolić ZM, Anđelković M, Savić T. Different responses of Drosophila subobscura isofemale lines to extremely low frequency magnetic field (50Hz, 0.5mT): fitness components and locomotor activity. in International Journal of Radiation Biology. 2017;93(5):544-552.
doi:10.1080/09553002.2017.1268281 .

Zmejkoski, Danica, Petković, Branka, Pavković-Lučić, Sofija, Prolić, Zlatko M., Anđelković, Marko, Savić, Tatjana, "Different responses of Drosophila subobscura isofemale lines to extremely low frequency magnetic field (50Hz, 0.5mT): fitness components and locomotor activity" in International Journal of Radiation Biology, 93, no. 5 (2017):544-552,
https://doi.org/10.1080/09553002.2017.1268281 . .

TY  - JOUR
AU  - Dmitrović, Svetlana
AU  - Jokić, Bojan M.
AU  - Prekajski, Marija D.
AU  - Pantić, Jelena R.
AU  - Zmejkoski, Danica
AU  - Zarubica, Aleksandra R.
AU  - Matović, Branko
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1172
AB  - Spider silk poses excellent mechanical properties, tenacity and elasticity and it has been used as a template for calcite mineralization to improve load bearing strength of osteoconductive calcite. The samples were obtained by mimicking biomineralization for five days in order to follow formation and growth of calcite on the surface of spider silk. Crystal phase was detected by XRD and FTIR spectroscopy. Microstructure, crystal size and its morphology were studied by means of FESEM. After two days of processing, pure calcite phase was obtained, and a size of the formed crystals increased with prolongation of biomineralization.
T2  - Processing and Application of Ceramics
T1  - Synthesis and characterization of spider silk calcite composite
VL  - 10
IS  - 1
SP  - 37
EP  - 40
DO  - 10.2298/PAC1601037D
ER  - 

@article{
author = "Dmitrović, Svetlana and Jokić, Bojan M. and Prekajski, Marija D. and Pantić, Jelena R. and Zmejkoski, Danica and Zarubica, Aleksandra R. and Matović, Branko",
year = "2016",
abstract = "Spider silk poses excellent mechanical properties, tenacity and elasticity and it has been used as a template for calcite mineralization to improve load bearing strength of osteoconductive calcite. The samples were obtained by mimicking biomineralization for five days in order to follow formation and growth of calcite on the surface of spider silk. Crystal phase was detected by XRD and FTIR spectroscopy. Microstructure, crystal size and its morphology were studied by means of FESEM. After two days of processing, pure calcite phase was obtained, and a size of the formed crystals increased with prolongation of biomineralization.",
journal = "Processing and Application of Ceramics",
title = "Synthesis and characterization of spider silk calcite composite",
volume = "10",
number = "1",
pages = "37-40",
doi = "10.2298/PAC1601037D"
}

Dmitrović, S., Jokić, B. M., Prekajski, M. D., Pantić, J. R., Zmejkoski, D., Zarubica, A. R.,& Matović, B.. (2016). Synthesis and characterization of spider silk calcite composite. in Processing and Application of Ceramics, 10(1), 37-40.
https://doi.org/10.2298/PAC1601037D

Dmitrović S, Jokić BM, Prekajski MD, Pantić JR, Zmejkoski D, Zarubica AR, Matović B. Synthesis and characterization of spider silk calcite composite. in Processing and Application of Ceramics. 2016;10(1):37-40.
doi:10.2298/PAC1601037D .

Dmitrović, Svetlana, Jokić, Bojan M., Prekajski, Marija D., Pantić, Jelena R., Zmejkoski, Danica, Zarubica, Aleksandra R., Matović, Branko, "Synthesis and characterization of spider silk calcite composite" in Processing and Application of Ceramics, 10, no. 1 (2016):37-40,
https://doi.org/10.2298/PAC1601037D . .

TY  - JOUR
AU  - Spasojević, Dragica
AU  - Zmejkoski, Danica
AU  - Glamočlija, Jasmina
AU  - Nikolić, Miloš M.
AU  - Soković, Marina
AU  - Milošević, Verica
AU  - Jarić, Ivana
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Barisani-Asenbauer, Talin
AU  - Prodanović, Radivoje
AU  - Jovanović, Miloš
AU  - Radotić, Ksenija
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1336
AB  - Nowadays bacterial resistance to known antibiotics is a serious health problem. In order to achieve more efficient treatment, lately there is an effort to find new substances, such as certain biomaterials, that are non-toxic to humans with antibiotic potential. Lignins and lignin-derived compounds have been proposed to be good candidates for use in medicine and health maintenance. In this study, the antibacterial activity of the lignin model polymer dehydrogenate polymer (DHP) in alginate hydrogel (Alg) was studied. The obtained results show that DHP-Alg has strong antimicrobial activity against several bacterial strains and biofilms and does not have a toxic effect on human epithelial cells. These results strongly suggest its application as a wound healing agent or as an adjunct substance for wound treatments. (C) 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.
T2  - International Journal of Antimicrobial Agents
T1  - Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment
VL  - 48
IS  - 6
SP  - 732
EP  - 735
DO  - 10.1016/j.ijantimicag.2016.08.014
ER  - 

@article{
author = "Spasojević, Dragica and Zmejkoski, Danica and Glamočlija, Jasmina and Nikolić, Miloš M. and Soković, Marina and Milošević, Verica and Jarić, Ivana and Stojanović, Marijana and Marinković, Emilija and Barisani-Asenbauer, Talin and Prodanović, Radivoje and Jovanović, Miloš and Radotić, Ksenija",
year = "2016",
abstract = "Nowadays bacterial resistance to known antibiotics is a serious health problem. In order to achieve more efficient treatment, lately there is an effort to find new substances, such as certain biomaterials, that are non-toxic to humans with antibiotic potential. Lignins and lignin-derived compounds have been proposed to be good candidates for use in medicine and health maintenance. In this study, the antibacterial activity of the lignin model polymer dehydrogenate polymer (DHP) in alginate hydrogel (Alg) was studied. The obtained results show that DHP-Alg has strong antimicrobial activity against several bacterial strains and biofilms and does not have a toxic effect on human epithelial cells. These results strongly suggest its application as a wound healing agent or as an adjunct substance for wound treatments. (C) 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.",
journal = "International Journal of Antimicrobial Agents",
title = "Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment",
volume = "48",
number = "6",
pages = "732-735",
doi = "10.1016/j.ijantimicag.2016.08.014"
}

Spasojević, D., Zmejkoski, D., Glamočlija, J., Nikolić, M. M., Soković, M., Milošević, V., Jarić, I., Stojanović, M., Marinković, E., Barisani-Asenbauer, T., Prodanović, R., Jovanović, M.,& Radotić, K.. (2016). Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment. in International Journal of Antimicrobial Agents, 48(6), 732-735.
https://doi.org/10.1016/j.ijantimicag.2016.08.014

Spasojević D, Zmejkoski D, Glamočlija J, Nikolić MM, Soković M, Milošević V, Jarić I, Stojanović M, Marinković E, Barisani-Asenbauer T, Prodanović R, Jovanović M, Radotić K. Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment. in International Journal of Antimicrobial Agents. 2016;48(6):732-735.
doi:10.1016/j.ijantimicag.2016.08.014 .

Spasojević, Dragica, Zmejkoski, Danica, Glamočlija, Jasmina, Nikolić, Miloš M., Soković, Marina, Milošević, Verica, Jarić, Ivana, Stojanović, Marijana, Marinković, Emilija, Barisani-Asenbauer, Talin, Prodanović, Radivoje, Jovanović, Miloš, Radotić, Ksenija, "Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment" in International Journal of Antimicrobial Agents, 48, no. 6 (2016):732-735,
https://doi.org/10.1016/j.ijantimicag.2016.08.014 . .

TY  - JOUR
AU  - Pešić, Milica
AU  - Podolski-Renić, Ana
AU  - Stojković, Sonja
AU  - Matović, Branko
AU  - Zmejkoski, Danica
AU  - Kojić, Vesna
AU  - Bogdanović, Gordana
AU  - Pavicevic, Aleksandra
AU  - Mojovic, Milos
AU  - Savić, Aleksandar
AU  - Milenković, Ivana
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/519
AB  - Data on medical applications of cerium oxide nanoparticles CeO2 (CONP) are promising, yet information regarding their action in cells is incomplete and there are conflicting reports about in vitro toxicity. Herein, we have studied cytotoxic effect of CONP in several cancer and normal cell lines and their potential to change intracellular redox status. The IC50 was achieved only in two of eight tested cell lines, melanoma 518A2 and colorectal adenocarcinoma HT-29. Self-propagating room temperature method was applied to produce CONP with an average crystalline size of 4 nm. The results confirmed presence of Ce3+ and O2- vacancies. The induction of cell death by CONP and the production of reactive oxygen species (ROS) were analyzed by flow-cytometry. Free radicals related antioxidant capacity of the cells was studied by the reduction of stable free radical TEMPONE using electron spin resonance spectroscopy. CONP showed low or moderate cytotoxicity in cancer cell lines: adenocarcinoma DLD1 and multi-drug resistant DLD1-TxR, non-small cell lung carcinoma NCI-H460 and multi-drug resistant NCI-H460/R, while normal cell lines (keratinocytes HaCaT, lung fetal fibroblasts MRC-5) were insensitive. The most sensitive were 518A2 melanoma and HT-29 colorectal adenocarcinoma cell lines, with the IC50 values being between 100 and 200 mu M. Decreased rate of TEMPONE reduction and increased production of certain ROS species (peroxynitrite and hydrogen peroxide anion) indicates that free radical metabolism, thus redox status was changed, and antioxidant capacity damaged in the CONP treated 518A2 and HT-29 cells. In conclusion, changes in intracellular redox status induced by CONP are partly attributed to the prooxidant activity of the nanoparticles. Further, ROS induced cell damages might eventually lead to the cell death. However, low inhibitory potential of CONP in the other human cell lines tested indicates that CONP may be safe for human usage in industry and medicine. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
T2  - Chemico-Biological Interactions
T1  - Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity
VL  - 232
SP  - 85
EP  - 93
DO  - 10.1016/j.cbi.2015.03.013
ER  - 

@article{
author = "Pešić, Milica and Podolski-Renić, Ana and Stojković, Sonja and Matović, Branko and Zmejkoski, Danica and Kojić, Vesna and Bogdanović, Gordana and Pavicevic, Aleksandra and Mojovic, Milos and Savić, Aleksandar and Milenković, Ivana and Kalauzi, Aleksandar and Radotić, Ksenija",
year = "2015",
abstract = "Data on medical applications of cerium oxide nanoparticles CeO2 (CONP) are promising, yet information regarding their action in cells is incomplete and there are conflicting reports about in vitro toxicity. Herein, we have studied cytotoxic effect of CONP in several cancer and normal cell lines and their potential to change intracellular redox status. The IC50 was achieved only in two of eight tested cell lines, melanoma 518A2 and colorectal adenocarcinoma HT-29. Self-propagating room temperature method was applied to produce CONP with an average crystalline size of 4 nm. The results confirmed presence of Ce3+ and O2- vacancies. The induction of cell death by CONP and the production of reactive oxygen species (ROS) were analyzed by flow-cytometry. Free radicals related antioxidant capacity of the cells was studied by the reduction of stable free radical TEMPONE using electron spin resonance spectroscopy. CONP showed low or moderate cytotoxicity in cancer cell lines: adenocarcinoma DLD1 and multi-drug resistant DLD1-TxR, non-small cell lung carcinoma NCI-H460 and multi-drug resistant NCI-H460/R, while normal cell lines (keratinocytes HaCaT, lung fetal fibroblasts MRC-5) were insensitive. The most sensitive were 518A2 melanoma and HT-29 colorectal adenocarcinoma cell lines, with the IC50 values being between 100 and 200 mu M. Decreased rate of TEMPONE reduction and increased production of certain ROS species (peroxynitrite and hydrogen peroxide anion) indicates that free radical metabolism, thus redox status was changed, and antioxidant capacity damaged in the CONP treated 518A2 and HT-29 cells. In conclusion, changes in intracellular redox status induced by CONP are partly attributed to the prooxidant activity of the nanoparticles. Further, ROS induced cell damages might eventually lead to the cell death. However, low inhibitory potential of CONP in the other human cell lines tested indicates that CONP may be safe for human usage in industry and medicine. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
journal = "Chemico-Biological Interactions",
title = "Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity",
volume = "232",
pages = "85-93",
doi = "10.1016/j.cbi.2015.03.013"
}

Pešić, M., Podolski-Renić, A., Stojković, S., Matović, B., Zmejkoski, D., Kojić, V., Bogdanović, G., Pavicevic, A., Mojovic, M., Savić, A., Milenković, I., Kalauzi, A.,& Radotić, K.. (2015). Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity. in Chemico-Biological Interactions, 232, 85-93.
https://doi.org/10.1016/j.cbi.2015.03.013

Pešić M, Podolski-Renić A, Stojković S, Matović B, Zmejkoski D, Kojić V, Bogdanović G, Pavicevic A, Mojovic M, Savić A, Milenković I, Kalauzi A, Radotić K. Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity. in Chemico-Biological Interactions. 2015;232:85-93.
doi:10.1016/j.cbi.2015.03.013 .

Pešić, Milica, Podolski-Renić, Ana, Stojković, Sonja, Matović, Branko, Zmejkoski, Danica, Kojić, Vesna, Bogdanović, Gordana, Pavicevic, Aleksandra, Mojovic, Milos, Savić, Aleksandar, Milenković, Ivana, Kalauzi, Aleksandar, Radotić, Ksenija, "Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity" in Chemico-Biological Interactions, 232 (2015):85-93,
https://doi.org/10.1016/j.cbi.2015.03.013 . .