TY  - JOUR
AU  - Mitrović, Kristina
AU  - Životić, Ivan
AU  - Kolić, Ivana
AU  - Žakula, Jelena
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12889
AB  - Background The majority of CAKUT-associated CNVs overlap at least one miRNA gene, thus affecting the cellular levels of the corresponding miRNA. We aimed to investigate the potency of restitution of CNV-affected miRNA levels to remediate the dysregulated expression of target genes involved in kidney physiology and development in vitro.  Methods Heterozygous MIR484 knockout HEK293 and homozygous MIR185 knockout HEK293 cell lines were used as models depicting the deletion of the frequently affected miRNA genes by CAKUT-associated CNVs. After treatment with the corresponding miRNA mimics, the levels of the target genes have been compared to the non-targeting control treatment. For both investigated miRNAs, MDM2 and PKD1 were evaluated as common targets, while additional 3 genes were investigated as targets of each individual miRNA (NOTCH3, FIS1 and APAF1 as hsa-miR-484 targets and RHOA, ATF6 and CDC42 as hsa-miR-185-5p targets).  Results Restitution of the corresponding miRNA levels in both knockout cell lines has induced a change in the mRNA levels of certain candidate target genes, thus confirming the potential to alleviate the CNV effect on miRNA expression. Intriguingly, HEK293 WT treatment with investigated miRNA mimics has triggered a more pronounced effect, thus suggesting the importance of miRNA interplay in different genomic contexts.  Conclusions Dysregulation of multiple mRNA targets mediated by CNV-affected miRNAs could represent the underlying mechanism behind the unresolved CAKUT occurrence and phenotypic variability observed in CAKUT patients. Characterizing miRNAs located in CNVs and their potential to become molecular targets could eventually help in understanding and improving the management of CAKUT.
T2  - BMC Genomics
T1  - A preliminary study of the miRNA restitution effect on CNV-induced miRNA downregulation in CAKUT
VL  - 25
IS  - 1
SP  - 218
DO  - 10.1186/s12864-024-10121-8
ER  - 

@article{
author = "Mitrović, Kristina and Životić, Ivan and Kolić, Ivana and Žakula, Jelena and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan",
year = "2024",
abstract = "Background The majority of CAKUT-associated CNVs overlap at least one miRNA gene, thus affecting the cellular levels of the corresponding miRNA. We aimed to investigate the potency of restitution of CNV-affected miRNA levels to remediate the dysregulated expression of target genes involved in kidney physiology and development in vitro.  Methods Heterozygous MIR484 knockout HEK293 and homozygous MIR185 knockout HEK293 cell lines were used as models depicting the deletion of the frequently affected miRNA genes by CAKUT-associated CNVs. After treatment with the corresponding miRNA mimics, the levels of the target genes have been compared to the non-targeting control treatment. For both investigated miRNAs, MDM2 and PKD1 were evaluated as common targets, while additional 3 genes were investigated as targets of each individual miRNA (NOTCH3, FIS1 and APAF1 as hsa-miR-484 targets and RHOA, ATF6 and CDC42 as hsa-miR-185-5p targets).  Results Restitution of the corresponding miRNA levels in both knockout cell lines has induced a change in the mRNA levels of certain candidate target genes, thus confirming the potential to alleviate the CNV effect on miRNA expression. Intriguingly, HEK293 WT treatment with investigated miRNA mimics has triggered a more pronounced effect, thus suggesting the importance of miRNA interplay in different genomic contexts.  Conclusions Dysregulation of multiple mRNA targets mediated by CNV-affected miRNAs could represent the underlying mechanism behind the unresolved CAKUT occurrence and phenotypic variability observed in CAKUT patients. Characterizing miRNAs located in CNVs and their potential to become molecular targets could eventually help in understanding and improving the management of CAKUT.",
journal = "BMC Genomics",
title = "A preliminary study of the miRNA restitution effect on CNV-induced miRNA downregulation in CAKUT",
volume = "25",
number = "1",
pages = "218",
doi = "10.1186/s12864-024-10121-8"
}

Mitrović, K., Životić, I., Kolić, I., Žakula, J., Živković, M., Stanković, A.,& Jovanović, I.. (2024). A preliminary study of the miRNA restitution effect on CNV-induced miRNA downregulation in CAKUT. in BMC Genomics, 25(1), 218.
https://doi.org/10.1186/s12864-024-10121-8

Mitrović K, Životić I, Kolić I, Žakula J, Živković M, Stanković A, Jovanović I. A preliminary study of the miRNA restitution effect on CNV-induced miRNA downregulation in CAKUT. in BMC Genomics. 2024;25(1):218.
doi:10.1186/s12864-024-10121-8 .

Mitrović, Kristina, Životić, Ivan, Kolić, Ivana, Žakula, Jelena, Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan, "A preliminary study of the miRNA restitution effect on CNV-induced miRNA downregulation in CAKUT" in BMC Genomics, 25, no. 1 (2024):218,
https://doi.org/10.1186/s12864-024-10121-8 . .

TY  - CONF
AU  - Životić, Ivan
AU  - Mitrović, Kristina
AU  - Kolić, Ivana
AU  - Seke, Mariana
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12465
AB  - Introduction: Congenital anomalies of the kidney and urinary tracts(CAKUT) are a diverse spectrum of defects with complex etiology and not fully explained genetic background. miRNA-containing copy number variants (CNVs) are described as genetic risk factor for the disease development. We aimed to identify miRNAs with the maximum regulatory coverage of previously reported differentially expressed genes in CAKUT tissue compared to controls and bioinformatically characterize a set of these miRNAs which are located in common CNVs. Methods: Differentially expressed genes were identified from ureter tissue transcriptome open data GSE83946 from 15 CAKUT patients and 7 healthy controls, generated in house previously. miRPathDB v2.0 was used for identification of miRNAs with maximum coverage of DEGs(miRNAs which complimentarily regulate all DEGs). Mapping of maximum coverage miRNAs onto common CNVs (frequency >0.2) was performed using UCSC genome browser and gnomAD database. miRNA mapping common CNVs were further bioinformatically analyzed using miRPathDB v2.0. Results: In a maximum coverage set of 50 miRNAs interacting with DEGs in CAKUT, we have identified 3 miRNA geneslocated in the common CNVs(hsa-miR-663b, hsa-miR-3180-3p and hsa-miR-1302). Using Reactome database we identified all three miRNAsto be significantly enriched in the pathway Neuronal System: -log(p-value)>2.326 for hsa-miR-1302; -log(p-value)>1.556 for hsa-miR-3180-3p; and -log(pvalue)>1.703 for hsa-miR-663b. Conclusion: CAKUT is characterized with variable penetrability and expressivity and often followed with other comorbiditiessuch as neurodevelopmental disorders. miRNAsinvolved in DEG networks and prone to CNV effects could present modulating factors of the disease phenotype. Further studies should provide additional evidence about hsa-miR-1302, hsa-miR-3180-3p and hsa-miR-663b involvements in CAKUT etiology
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts
T1  - Identification of micro RNA from common copy number variants as risk factors for CAKUT
SP  - 62
EP  - 62
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12465
ER  - 

@conference{
author = "Životić, Ivan and Mitrović, Kristina and Kolić, Ivana and Seke, Mariana and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan",
year = "2023",
abstract = "Introduction: Congenital anomalies of the kidney and urinary tracts(CAKUT) are a diverse spectrum of defects with complex etiology and not fully explained genetic background. miRNA-containing copy number variants (CNVs) are described as genetic risk factor for the disease development. We aimed to identify miRNAs with the maximum regulatory coverage of previously reported differentially expressed genes in CAKUT tissue compared to controls and bioinformatically characterize a set of these miRNAs which are located in common CNVs. Methods: Differentially expressed genes were identified from ureter tissue transcriptome open data GSE83946 from 15 CAKUT patients and 7 healthy controls, generated in house previously. miRPathDB v2.0 was used for identification of miRNAs with maximum coverage of DEGs(miRNAs which complimentarily regulate all DEGs). Mapping of maximum coverage miRNAs onto common CNVs (frequency >0.2) was performed using UCSC genome browser and gnomAD database. miRNA mapping common CNVs were further bioinformatically analyzed using miRPathDB v2.0. Results: In a maximum coverage set of 50 miRNAs interacting with DEGs in CAKUT, we have identified 3 miRNA geneslocated in the common CNVs(hsa-miR-663b, hsa-miR-3180-3p and hsa-miR-1302). Using Reactome database we identified all three miRNAsto be significantly enriched in the pathway Neuronal System: -log(p-value)>2.326 for hsa-miR-1302; -log(p-value)>1.556 for hsa-miR-3180-3p; and -log(pvalue)>1.703 for hsa-miR-663b. Conclusion: CAKUT is characterized with variable penetrability and expressivity and often followed with other comorbiditiessuch as neurodevelopmental disorders. miRNAsinvolved in DEG networks and prone to CNV effects could present modulating factors of the disease phenotype. Further studies should provide additional evidence about hsa-miR-1302, hsa-miR-3180-3p and hsa-miR-663b involvements in CAKUT etiology",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts",
title = "Identification of micro RNA from common copy number variants as risk factors for CAKUT",
pages = "62-62",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12465"
}

Životić, I., Mitrović, K., Kolić, I., Seke, M., Živković, M., Stanković, A.,& Jovanović, I.. (2023). Identification of micro RNA from common copy number variants as risk factors for CAKUT. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts, 62-62.
https://hdl.handle.net/21.15107/rcub_vinar_12465

Životić I, Mitrović K, Kolić I, Seke M, Živković M, Stanković A, Jovanović I. Identification of micro RNA from common copy number variants as risk factors for CAKUT. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts. 2023;:62-62.
https://hdl.handle.net/21.15107/rcub_vinar_12465 .

Životić, Ivan, Mitrović, Kristina, Kolić, Ivana, Seke, Mariana, Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan, "Identification of micro RNA from common copy number variants as risk factors for CAKUT" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts (2023):62-62,
https://hdl.handle.net/21.15107/rcub_vinar_12465 .

TY  - CONF
AU  - Živković, Maja
AU  - Kostić, S.
AU  - Stojković, Ljiljana
AU  - Kolić, Ivana
AU  - Stanković, Aleksandra
AU  - Dinčić, E.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12466
AB  - Last decade provided multiple evidence that link disturbances in metabolic processes and energy metabolism with diseases of central nervous system and neurodegeneration. Initial phases of insulin resistance (IR) are present in natural course of multiple sclerosis (MS) and leptin was recognized as a player in MS pathophysiology and moreover cognitive decline. We aimed to investigate association of genetic variants in leptin (LEP) rs7799039, its receptor LEPR rs1137101 and proliferator-activated receptor gamma co-activator 1-alpha (PGCA1A) rs8192678 with IR parameters (HOMA-IR index, area under the curve for insulin and glucose, Cederholm insulin sensitivity index (ISIced), the insulinogenic index in the first 30 min of oral glucose tolerance test (OGTT) in patients with MS. Seventy eight relapsing-remitting patients in clinical remission, free of corticosteroids for at least three months, were included in the study. None of the 3 variants’ genotypes were associated with HOMA-IR index, area under the curve for insulin and glucose and the insulinogenic index in the first 30 min of OGTT. PGC1A variant was significantly associated with ISIced (Kruskal-Wallis ANOVA, p = 0.04). Leptin gene variant was significantly associated with impaired GT (p=0.029 adjusted for gender and other two variants). None of the variant showed association with IR. In conclusion, we found that genetic variants in leptin signalling pathway affect glucose tolerance and insulin sensitivity in patients with MS. As both, leptin and PGC1A have role in preventing neuronal death and reducing oxidative stress neuronal damage current results favour further investigation toward preserving cognitive status and neuroprotection in MS.
C3  - CONy : 17th World Congress on Controversies in Neurology : Abstract book
T1  - Leptin signalling genetic variants and insulin resistance in multiple sclerosis patients
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12466
ER  - 

@conference{
author = "Živković, Maja and Kostić, S. and Stojković, Ljiljana and Kolić, Ivana and Stanković, Aleksandra and Dinčić, E.",
year = "2023",
abstract = "Last decade provided multiple evidence that link disturbances in metabolic processes and energy metabolism with diseases of central nervous system and neurodegeneration. Initial phases of insulin resistance (IR) are present in natural course of multiple sclerosis (MS) and leptin was recognized as a player in MS pathophysiology and moreover cognitive decline. We aimed to investigate association of genetic variants in leptin (LEP) rs7799039, its receptor LEPR rs1137101 and proliferator-activated receptor gamma co-activator 1-alpha (PGCA1A) rs8192678 with IR parameters (HOMA-IR index, area under the curve for insulin and glucose, Cederholm insulin sensitivity index (ISIced), the insulinogenic index in the first 30 min of oral glucose tolerance test (OGTT) in patients with MS. Seventy eight relapsing-remitting patients in clinical remission, free of corticosteroids for at least three months, were included in the study. None of the 3 variants’ genotypes were associated with HOMA-IR index, area under the curve for insulin and glucose and the insulinogenic index in the first 30 min of OGTT. PGC1A variant was significantly associated with ISIced (Kruskal-Wallis ANOVA, p = 0.04). Leptin gene variant was significantly associated with impaired GT (p=0.029 adjusted for gender and other two variants). None of the variant showed association with IR. In conclusion, we found that genetic variants in leptin signalling pathway affect glucose tolerance and insulin sensitivity in patients with MS. As both, leptin and PGC1A have role in preventing neuronal death and reducing oxidative stress neuronal damage current results favour further investigation toward preserving cognitive status and neuroprotection in MS.",
journal = "CONy : 17th World Congress on Controversies in Neurology : Abstract book",
title = "Leptin signalling genetic variants and insulin resistance in multiple sclerosis patients",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12466"
}

Živković, M., Kostić, S., Stojković, L., Kolić, I., Stanković, A.,& Dinčić, E.. (2023). Leptin signalling genetic variants and insulin resistance in multiple sclerosis patients. in CONy : 17th World Congress on Controversies in Neurology : Abstract book.
https://hdl.handle.net/21.15107/rcub_vinar_12466

Živković M, Kostić S, Stojković L, Kolić I, Stanković A, Dinčić E. Leptin signalling genetic variants and insulin resistance in multiple sclerosis patients. in CONy : 17th World Congress on Controversies in Neurology : Abstract book. 2023;.
https://hdl.handle.net/21.15107/rcub_vinar_12466 .

Živković, Maja, Kostić, S., Stojković, Ljiljana, Kolić, Ivana, Stanković, Aleksandra, Dinčić, E., "Leptin signalling genetic variants and insulin resistance in multiple sclerosis patients" in CONy : 17th World Congress on Controversies in Neurology : Abstract book (2023),
https://hdl.handle.net/21.15107/rcub_vinar_12466 .

TY  - JOUR
AU  - Mitrović, Kristina
AU  - Životić, Ivan
AU  - Kolić, Ivana
AU  - Đorđević, Ana
AU  - Žakula, Jelena
AU  - Filipović Tričković, Jelena G.
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan G.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10469
AB  - Rare copy number variants (CNVs) are among the most common genomic disorders underlying CAKUT. miRNAs located in rare CNVs represent well-founded functional variants for human CAKUT research. The study aimed to identify and functionally interpret miRNAs most frequently affected by rare CNVs in CAKUT and to estimate the overall burden of rare CNVs on miRNA genes in CAKUT. The additional aim of this study was to experimentally confirm the effect of a rare CNV in CAKUT on candidate miRNA’s expression and the subsequent change in mRNA levels of selected target genes. A database of CAKUT-associated rare CNV regions, created by literature mining, was used for mapping of the miRNA precursors. miRNAs and miRNA families, most frequently affected by rare CAKUT-associated CNVs, have been subjected to bioinformatic analysis. CNV burden analysis was performed to identify chromosomes with over/underrepresentation of miRNA genes in rare CNVs associated with CAKUT. A functional study was performed on HEK293 MIR484+/- KO and HEK293 WT cell lines, followed by the analysis of relative miRNA and mRNA target gene levels. 80% of CAKUT patients with underlying rare CNV had at least one miRNA gene overlapping the identified CNV. Network analysis of the most frequently affected miRNAs has revealed the dominant regulation of the two miRNAs, hsa-miR-484 and hsa-miR-185-5p. Additionally, miR-548 family members have shown substantial enrichment in rare CNVs in CAKUT. An over/underrepresentation of miRNA genes in rare CNVs associated with CAKUT was observed in multiple chromosomes, such as chr16, chr20, and chr21. A significant 0.37 fold downregulation of hsa-miR-484, followed by a notable upregulation of MDM2 and APAF1 and downregulation of NOTCH3 was detected in HEK293 MIR484+/- KO compared to HEK293 WT cell lines, supporting the study hypothesis. miRNA genes are frequently affected by rare CNVs in CAKUT patients. Understanding the potential of CNV-affected miRNAs to participate in CAKUT as genetic drivers represent a crucial implication for the development of novel therapeutic approaches.
T2  - Scientific Reports
T1  - Identification and functional interpretation of miRNAs affected by rare CNVs in CAKUT
VL  - 12
IS  - 1
SP  - 17746
DO  - 10.1038/s41598-022-22749-1
ER  - 

@article{
author = "Mitrović, Kristina and Životić, Ivan and Kolić, Ivana and Đorđević, Ana and Žakula, Jelena and Filipović Tričković, Jelena G. and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan G.",
year = "2022",
abstract = "Rare copy number variants (CNVs) are among the most common genomic disorders underlying CAKUT. miRNAs located in rare CNVs represent well-founded functional variants for human CAKUT research. The study aimed to identify and functionally interpret miRNAs most frequently affected by rare CNVs in CAKUT and to estimate the overall burden of rare CNVs on miRNA genes in CAKUT. The additional aim of this study was to experimentally confirm the effect of a rare CNV in CAKUT on candidate miRNA’s expression and the subsequent change in mRNA levels of selected target genes. A database of CAKUT-associated rare CNV regions, created by literature mining, was used for mapping of the miRNA precursors. miRNAs and miRNA families, most frequently affected by rare CAKUT-associated CNVs, have been subjected to bioinformatic analysis. CNV burden analysis was performed to identify chromosomes with over/underrepresentation of miRNA genes in rare CNVs associated with CAKUT. A functional study was performed on HEK293 MIR484+/- KO and HEK293 WT cell lines, followed by the analysis of relative miRNA and mRNA target gene levels. 80% of CAKUT patients with underlying rare CNV had at least one miRNA gene overlapping the identified CNV. Network analysis of the most frequently affected miRNAs has revealed the dominant regulation of the two miRNAs, hsa-miR-484 and hsa-miR-185-5p. Additionally, miR-548 family members have shown substantial enrichment in rare CNVs in CAKUT. An over/underrepresentation of miRNA genes in rare CNVs associated with CAKUT was observed in multiple chromosomes, such as chr16, chr20, and chr21. A significant 0.37 fold downregulation of hsa-miR-484, followed by a notable upregulation of MDM2 and APAF1 and downregulation of NOTCH3 was detected in HEK293 MIR484+/- KO compared to HEK293 WT cell lines, supporting the study hypothesis. miRNA genes are frequently affected by rare CNVs in CAKUT patients. Understanding the potential of CNV-affected miRNAs to participate in CAKUT as genetic drivers represent a crucial implication for the development of novel therapeutic approaches.",
journal = "Scientific Reports",
title = "Identification and functional interpretation of miRNAs affected by rare CNVs in CAKUT",
volume = "12",
number = "1",
pages = "17746",
doi = "10.1038/s41598-022-22749-1"
}

Mitrović, K., Životić, I., Kolić, I., Đorđević, A., Žakula, J., Filipović Tričković, J. G., Živković, M., Stanković, A.,& Jovanović, I. G.. (2022). Identification and functional interpretation of miRNAs affected by rare CNVs in CAKUT. in Scientific Reports, 12(1), 17746.
https://doi.org/10.1038/s41598-022-22749-1

Mitrović K, Životić I, Kolić I, Đorđević A, Žakula J, Filipović Tričković JG, Živković M, Stanković A, Jovanović IG. Identification and functional interpretation of miRNAs affected by rare CNVs in CAKUT. in Scientific Reports. 2022;12(1):17746.
doi:10.1038/s41598-022-22749-1 .

Mitrović, Kristina, Životić, Ivan, Kolić, Ivana, Đorđević, Ana, Žakula, Jelena, Filipović Tričković, Jelena G., Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan G., "Identification and functional interpretation of miRNAs affected by rare CNVs in CAKUT" in Scientific Reports, 12, no. 1 (2022):17746,
https://doi.org/10.1038/s41598-022-22749-1 . .

TY  - CONF
AU  - Mitrović, Kristina
AU  - Kolić, Ivana
AU  - Životić, Ivan
AU  - Filipović Tričković, Jelena G.
AU  - Đorđević, Ana
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan G.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10830
AB  - Introduction: miR-548 family members, located on all human chromosomes except chr19 and chrY, regulate podocyte differentiation in vitro, important for kidney development. Rare copy number variants (rCNVs) are the common genetic cause of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT) and could harbour miRNAs. The aim of this study was to investigate to which extent rCNVs associated with CAKUT harbour miR-548 members.
Materials and Methods: Extensive literature review was conducted to collect data of pathogenic and likely pathogenic rCNVs in CAKUT patients. UCSC genome browser tool was employed for mapping of miR-548 members onto collected rCNV regions and gnomAD SV controls database. Bioinformatic analysis was conducted using miRPathDB2 tool.
Results: We generated CAKUT database of pathogenic CNVs in 79 chromosome regions from 191 patient and likely pathogenic CNVs in 74 regions from 87 patients. Pathogenic rCNVs of seventeen patients, located on 7 chromosomes, contained at least one miR-548 member. Likely pathogenic rCNVs of 4 patients, located on 3 chromosomes, contained one of miR-548 members. Bioinformatic analysis implied the role of mapped miRNAs in the regulation of processes associated with CAKUT. In controls, only hsa-mir-548i-3 (out of 73 precursors) was mapped on polymorphic CNVs (af>1%) and wasn’t identified in patients.
Conclusions: miR-548 members located in rCNVs should be investigated in future studies as potential genetic drivers of CAKUT development, beyond protein coding genes.
C3  - European Journal of Human Genetics
T1  - Are miR-548 family members potential genetic drivers of CAKUT
VL  - 30
IS  - Suppl. 1
SP  - 331
DO  - 10.1038/s41431-021-01026-1
ER  - 

@conference{
author = "Mitrović, Kristina and Kolić, Ivana and Životić, Ivan and Filipović Tričković, Jelena G. and Đorđević, Ana and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan G.",
year = "2022",
abstract = "Introduction: miR-548 family members, located on all human chromosomes except chr19 and chrY, regulate podocyte differentiation in vitro, important for kidney development. Rare copy number variants (rCNVs) are the common genetic cause of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT) and could harbour miRNAs. The aim of this study was to investigate to which extent rCNVs associated with CAKUT harbour miR-548 members.
Materials and Methods: Extensive literature review was conducted to collect data of pathogenic and likely pathogenic rCNVs in CAKUT patients. UCSC genome browser tool was employed for mapping of miR-548 members onto collected rCNV regions and gnomAD SV controls database. Bioinformatic analysis was conducted using miRPathDB2 tool.
Results: We generated CAKUT database of pathogenic CNVs in 79 chromosome regions from 191 patient and likely pathogenic CNVs in 74 regions from 87 patients. Pathogenic rCNVs of seventeen patients, located on 7 chromosomes, contained at least one miR-548 member. Likely pathogenic rCNVs of 4 patients, located on 3 chromosomes, contained one of miR-548 members. Bioinformatic analysis implied the role of mapped miRNAs in the regulation of processes associated with CAKUT. In controls, only hsa-mir-548i-3 (out of 73 precursors) was mapped on polymorphic CNVs (af>1%) and wasn’t identified in patients.
Conclusions: miR-548 members located in rCNVs should be investigated in future studies as potential genetic drivers of CAKUT development, beyond protein coding genes.",
journal = "European Journal of Human Genetics",
title = "Are miR-548 family members potential genetic drivers of CAKUT",
volume = "30",
number = "Suppl. 1",
pages = "331",
doi = "10.1038/s41431-021-01026-1"
}

Mitrović, K., Kolić, I., Životić, I., Filipović Tričković, J. G., Đorđević, A., Živković, M., Stanković, A.,& Jovanović, I. G.. (2022). Are miR-548 family members potential genetic drivers of CAKUT. in European Journal of Human Genetics, 30(Suppl. 1), 331.
https://doi.org/10.1038/s41431-021-01026-1

Mitrović K, Kolić I, Životić I, Filipović Tričković JG, Đorđević A, Živković M, Stanković A, Jovanović IG. Are miR-548 family members potential genetic drivers of CAKUT. in European Journal of Human Genetics. 2022;30(Suppl. 1):331.
doi:10.1038/s41431-021-01026-1 .

Mitrović, Kristina, Kolić, Ivana, Životić, Ivan, Filipović Tričković, Jelena G., Đorđević, Ana, Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan G., "Are miR-548 family members potential genetic drivers of CAKUT" in European Journal of Human Genetics, 30, no. Suppl. 1 (2022):331,
https://doi.org/10.1038/s41431-021-01026-1 . .

TY  - CONF
AU  - Životić, Ivan
AU  - Kolić, Ivana
AU  - Popić, Kristina
AU  - Filipović Tričković, Jelena G.
AU  - Đorđević, Ana
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan G.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10831
AB  - Introduction: Genetic studies of congenital anomalies of the kidney and urinary tract (CAKUT) have demonstrated variable penetrability and expressivity of the associated genetic defects. Previously, it was shown that deletions of 17q12 and 22q11.2 regions were specific for kidney anomalies (KA) while 16p11.2 and 1q21.1 loci showed extensive pleiotropy in CAKUT phenotypes. CNVs affecting miRNA gene dosage have been described to have functional influence on gene expression. We aimed to conduct comprehensive in silico analysis using publicly available databases to analyze miRNA content of CAKUT-associated CNVs in quoted chromosomal loci with regard to pleiotropy.  Methods: Extensive literature review was conducted to collect data about pathogenic rCNVs associated with CAKUT. UCSC genome browser tool was employed for mapping miRNAs onto collected rCNV regions.  Results: Analysis of CNVs in CAKUT included four studies counting more than 2500 patients. In further analysis we included 191 patients harboring pathogenic CNVs. Surprisingly, CAKUT pleiotropic regions (16p11.2, 1q21.2) did not contain any miRNA. 22q11.2 showed the densest miRNAs content (n = 21).  Conclusions: Absence of miRNAs may potentially pronounce the pleiotropy of the CAKUT genetic defects, thus leading to the variety of phenotypes. Contrary, abundancy of miRNAs in 22q11.2 might be associated with reproducible phenotype, such as KA, producing the functional effect when deleted. This assumption agrees with recent results of miRNA expression variability in 22q11.2 deletion syndrome.
C3  - European Journal of Human Genetics
T1  - miRNA-free rare pathogenic CNVs could drive toward variable CAKUT phenotypes
VL  - 30
IS  - Suppl. 1
SP  - 331
DO  - 10.1038/s41431-021-01026-1
ER  - 

@conference{
author = "Životić, Ivan and Kolić, Ivana and Popić, Kristina and Filipović Tričković, Jelena G. and Đorđević, Ana and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan G.",
year = "2022",
abstract = "Introduction: Genetic studies of congenital anomalies of the kidney and urinary tract (CAKUT) have demonstrated variable penetrability and expressivity of the associated genetic defects. Previously, it was shown that deletions of 17q12 and 22q11.2 regions were specific for kidney anomalies (KA) while 16p11.2 and 1q21.1 loci showed extensive pleiotropy in CAKUT phenotypes. CNVs affecting miRNA gene dosage have been described to have functional influence on gene expression. We aimed to conduct comprehensive in silico analysis using publicly available databases to analyze miRNA content of CAKUT-associated CNVs in quoted chromosomal loci with regard to pleiotropy.  Methods: Extensive literature review was conducted to collect data about pathogenic rCNVs associated with CAKUT. UCSC genome browser tool was employed for mapping miRNAs onto collected rCNV regions.  Results: Analysis of CNVs in CAKUT included four studies counting more than 2500 patients. In further analysis we included 191 patients harboring pathogenic CNVs. Surprisingly, CAKUT pleiotropic regions (16p11.2, 1q21.2) did not contain any miRNA. 22q11.2 showed the densest miRNAs content (n = 21).  Conclusions: Absence of miRNAs may potentially pronounce the pleiotropy of the CAKUT genetic defects, thus leading to the variety of phenotypes. Contrary, abundancy of miRNAs in 22q11.2 might be associated with reproducible phenotype, such as KA, producing the functional effect when deleted. This assumption agrees with recent results of miRNA expression variability in 22q11.2 deletion syndrome.",
journal = "European Journal of Human Genetics",
title = "miRNA-free rare pathogenic CNVs could drive toward variable CAKUT phenotypes",
volume = "30",
number = "Suppl. 1",
pages = "331",
doi = "10.1038/s41431-021-01026-1"
}

Životić, I., Kolić, I., Popić, K., Filipović Tričković, J. G., Đorđević, A., Živković, M., Stanković, A.,& Jovanović, I. G.. (2022). miRNA-free rare pathogenic CNVs could drive toward variable CAKUT phenotypes. in European Journal of Human Genetics, 30(Suppl. 1), 331.
https://doi.org/10.1038/s41431-021-01026-1

Životić I, Kolić I, Popić K, Filipović Tričković JG, Đorđević A, Živković M, Stanković A, Jovanović IG. miRNA-free rare pathogenic CNVs could drive toward variable CAKUT phenotypes. in European Journal of Human Genetics. 2022;30(Suppl. 1):331.
doi:10.1038/s41431-021-01026-1 .

Životić, Ivan, Kolić, Ivana, Popić, Kristina, Filipović Tričković, Jelena G., Đorđević, Ana, Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan G., "miRNA-free rare pathogenic CNVs could drive toward variable CAKUT phenotypes" in European Journal of Human Genetics, 30, no. Suppl. 1 (2022):331,
https://doi.org/10.1038/s41431-021-01026-1 . .

TY  - CONF
AU  - Kolić, Ivana
AU  - Mitrović, Kristina
AU  - Životić, Ivan
AU  - Đorđević, Ana
AU  - Filipović-Tričković, Jelena
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12684
AB  - Background/Objectives: Rare copy number variants (rCNVs) are the common genetic cause of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT).1 miRNAs located in rCNVs represent well-founded functional variants for human CAKUT research. However, the impact of rCNVs on miRNA genes in CAKUT is unknown. Thus, burden assessment was performed to identify chromosomes with non-random representation of miRNA genes in rCNVs associated with CAKUT. Methods: A comprehensive literature mining of rCNV regions associated with CAKUT was performed. The total cumulative length of rCNVs per chromosome was the sum of corresponding CNV-DNA regions, taking into account overlapping. Mapping of miRNAs onto cumulative rCNV regions gave counts of affected miRNA loci. The correlation analysis was performed between the number of miRNA genes overlapping rCNVs, and the fractional lengths of cumulative rCNVs regions in relation to the chromosome size. Results: A statistically significant positive correlation was observed for duplications and deletions respectively (Spearman correlation p<0.0001, r=0.9, r=0.8). However, a deviation from the best fit line for chromosome 16, for both rare duplications and deletions, was observed due to the high overrepresentation of miRNA genes in identified rCNVs. Conclusion: The current finding of the high overall burden of rCNVs on miRNA genes in chromosome 16 suggests that miRNAs located on this chromosome could serve as candidates for the investigation of miRNA role in CAKUT development.
C3  - 54th European Society of Human Genetics (ESHG) : Book of abstracts
T1  - Assessing the burden of rare CNVs on miRNA genes in CAKUT
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12684
ER  - 

@conference{
author = "Kolić, Ivana and Mitrović, Kristina and Životić, Ivan and Đorđević, Ana and Filipović-Tričković, Jelena and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan",
year = "2022",
abstract = "Background/Objectives: Rare copy number variants (rCNVs) are the common genetic cause of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT).1 miRNAs located in rCNVs represent well-founded functional variants for human CAKUT research. However, the impact of rCNVs on miRNA genes in CAKUT is unknown. Thus, burden assessment was performed to identify chromosomes with non-random representation of miRNA genes in rCNVs associated with CAKUT. Methods: A comprehensive literature mining of rCNV regions associated with CAKUT was performed. The total cumulative length of rCNVs per chromosome was the sum of corresponding CNV-DNA regions, taking into account overlapping. Mapping of miRNAs onto cumulative rCNV regions gave counts of affected miRNA loci. The correlation analysis was performed between the number of miRNA genes overlapping rCNVs, and the fractional lengths of cumulative rCNVs regions in relation to the chromosome size. Results: A statistically significant positive correlation was observed for duplications and deletions respectively (Spearman correlation p<0.0001, r=0.9, r=0.8). However, a deviation from the best fit line for chromosome 16, for both rare duplications and deletions, was observed due to the high overrepresentation of miRNA genes in identified rCNVs. Conclusion: The current finding of the high overall burden of rCNVs on miRNA genes in chromosome 16 suggests that miRNAs located on this chromosome could serve as candidates for the investigation of miRNA role in CAKUT development.",
journal = "54th European Society of Human Genetics (ESHG) : Book of abstracts",
title = "Assessing the burden of rare CNVs on miRNA genes in CAKUT",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12684"
}

Kolić, I., Mitrović, K., Životić, I., Đorđević, A., Filipović-Tričković, J., Živković, M., Stanković, A.,& Jovanović, I.. (2022). Assessing the burden of rare CNVs on miRNA genes in CAKUT. in 54th European Society of Human Genetics (ESHG) : Book of abstracts.
https://hdl.handle.net/21.15107/rcub_vinar_12684

Kolić I, Mitrović K, Životić I, Đorđević A, Filipović-Tričković J, Živković M, Stanković A, Jovanović I. Assessing the burden of rare CNVs on miRNA genes in CAKUT. in 54th European Society of Human Genetics (ESHG) : Book of abstracts. 2022;.
https://hdl.handle.net/21.15107/rcub_vinar_12684 .

Kolić, Ivana, Mitrović, Kristina, Životić, Ivan, Đorđević, Ana, Filipović-Tričković, Jelena, Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan, "Assessing the burden of rare CNVs on miRNA genes in CAKUT" in 54th European Society of Human Genetics (ESHG) : Book of abstracts (2022),
https://hdl.handle.net/21.15107/rcub_vinar_12684 .

TY  - THES
AU  - Kolić, Ivana
PY  - 2021
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=8396
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:24612/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=52836361
UR  - https://nardus.mpn.gov.rs/handle/123456789/18742
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11443
AB  - Hormon i adipocitokin leptin (LEP) može učestvovati u patogenezi multiple skleroze (MS) delovanjem na produkciju potentnog proinflamatornog citokina, TNF-α, aktivnost transkripcionog koaktivatora PGC1A i produkciju ključnog enzima antioksidativne zaštite, SOD2. Predmet studije je genetičko-epidemiološka analiza asocijacije varijanti LEP rs7799039, LEPR rs1137101 i PGC1A rs8192678 i nivoa transkripcije gena LEP signalnog puta (LEP, LEPR i PGC1A), antioksidativne zaštite (SOD2) i inflamacije (TNF-α) sa nastankom i kliničkim tokom (relapsno-remitentna, RR i sekundarno progresivna, SP) MS. Genotipizacija varijanti i određivanje relativnih nivoa ciljnih iRNK u perifernim mononuklearnim leukocitima urađeni su metodom kvantitativne lančane reakcije polimeraze (qPCR) uz primenu TaqMan® eseja. Analizom tri ispitivane varijante u multiploj logističkoj regresiji, po dominantnom modelu nasleđivanja, utvrđen je povišen rizik za nastanak MS kod nosilaca ređeg alela, A, varijante u PGC1A. Kod muškaraca sa MS, ustanovljena je asocijacija ređeg alela, G, varijante u LEPR sa značajno povišenim vrednostima kliničkog parametra MSSS, u odnosu na muškarce sa genotipom AA, što sugeriše na potencijalni polno-zavisni uticaj varijante na težinu bolesti. Nivo LEP iRNK je značajno povišen, a nivoi LEPR i PGC1A iRNK značajno sniženi kod pacijenata sa RR MS, u odnosu na kontrole, što ukazuje na povezanost promena transkripcije ovih gena sa nastankom RR MS. Utvrđenom porastu nivoa LEP iRNK kod pacijenata može doprineti ređi alel, A, varijante u LEP. Aktivnost gena može biti povezana sa težinom bolesti, pošto je pokazano da LEPR iRNK i MSSS pozitivno korelišu. Rezultati studije ukazuju da varijante i promene nivoa iRNK gena LEP, LEPR i PGC1A mogu da utiču na nastanak i težinu kliničke slike MS.
AB  - Hormone and adipocytokine leptin (LEP) may participate in the pathogenesis of multiple sclerosis (MS) by affecting the production of a potent proinflammatory cytokine, TNF-α, activity of transcription coactivator PGC1A and production of a key antioxidant enzyme, SOD2. The objective of the study was genetic epidemiological analysis of association of LEP rs7799039, LEPR rs1137101 and PGC1A rs8192678 variants and transcription levels of genes of LEP signaling pathway (LEP, LEPR and PGC1A), antioxidant protection (SOD2) and inflammation (TNF-α), with the onset risk and clinical course (relapsing-remitting, RR and secondary progressive, SP) of MS. Genotyping of variants and determination of relative levels of target mRNAs in peripheral mononuclear leukocytes were performed by quantitative polymerase chain reaction (qPCR) using TaqMan® assays. Multiple logistic regression of three examined variants, by dominant model of inheritance, revealed an increased risk of MS in carriers of minor, A, allele of PGC1A variant. In men with MS, LEPR minor, G, allele was associated with higher values of the clinical parameter MSSS, compared to men with genotype AA, suggesting a potential sex-dependent effect of LEPR variant on disease severity. LEP mRNA was increased, while LEPR and PGC1A mRNA levels were decreased in patients with RR MS, compared to controls, indicating the association of transcription changes of these genes with the development of RR MS. LEP minor, A, allele may contribute to the determined increase in LEP mRNA levels in patients. Gene activity may be linked with disease severity, as LEPR mRNA and MSSS were shown to correlate. The results indicate that variants and changes in transcription levels of LEP, LEPR and PGC1A genes may affect the onset and severity of MS.Key words: multiple sclerosis, gene, variant, transcription, LEP, LEPR, PGC1A, SOD2, TNF-α
PB  - Универзитет у Београду, Биолошки факултет
T2  - Универзитет у Београду
T1  - Studija asocijacije varijanti i nivoa transkripcije gena leptinskog signalnog puta (LEP, LEPR i PGC1A), antioksidativne zaštite (SOD2) i inflamacije (TNF-α) sa rizikom za nastanak i kliničkim tokom multiple skleroze
UR  - https://hdl.handle.net/21.15107/rcub_nardus_18742
ER  - 

@phdthesis{
author = "Kolić, Ivana",
year = "2021",
abstract = "Hormon i adipocitokin leptin (LEP) može učestvovati u patogenezi multiple skleroze (MS) delovanjem na produkciju potentnog proinflamatornog citokina, TNF-α, aktivnost transkripcionog koaktivatora PGC1A i produkciju ključnog enzima antioksidativne zaštite, SOD2. Predmet studije je genetičko-epidemiološka analiza asocijacije varijanti LEP rs7799039, LEPR rs1137101 i PGC1A rs8192678 i nivoa transkripcije gena LEP signalnog puta (LEP, LEPR i PGC1A), antioksidativne zaštite (SOD2) i inflamacije (TNF-α) sa nastankom i kliničkim tokom (relapsno-remitentna, RR i sekundarno progresivna, SP) MS. Genotipizacija varijanti i određivanje relativnih nivoa ciljnih iRNK u perifernim mononuklearnim leukocitima urađeni su metodom kvantitativne lančane reakcije polimeraze (qPCR) uz primenu TaqMan® eseja. Analizom tri ispitivane varijante u multiploj logističkoj regresiji, po dominantnom modelu nasleđivanja, utvrđen je povišen rizik za nastanak MS kod nosilaca ređeg alela, A, varijante u PGC1A. Kod muškaraca sa MS, ustanovljena je asocijacija ređeg alela, G, varijante u LEPR sa značajno povišenim vrednostima kliničkog parametra MSSS, u odnosu na muškarce sa genotipom AA, što sugeriše na potencijalni polno-zavisni uticaj varijante na težinu bolesti. Nivo LEP iRNK je značajno povišen, a nivoi LEPR i PGC1A iRNK značajno sniženi kod pacijenata sa RR MS, u odnosu na kontrole, što ukazuje na povezanost promena transkripcije ovih gena sa nastankom RR MS. Utvrđenom porastu nivoa LEP iRNK kod pacijenata može doprineti ređi alel, A, varijante u LEP. Aktivnost gena može biti povezana sa težinom bolesti, pošto je pokazano da LEPR iRNK i MSSS pozitivno korelišu. Rezultati studije ukazuju da varijante i promene nivoa iRNK gena LEP, LEPR i PGC1A mogu da utiču na nastanak i težinu kliničke slike MS., Hormone and adipocytokine leptin (LEP) may participate in the pathogenesis of multiple sclerosis (MS) by affecting the production of a potent proinflammatory cytokine, TNF-α, activity of transcription coactivator PGC1A and production of a key antioxidant enzyme, SOD2. The objective of the study was genetic epidemiological analysis of association of LEP rs7799039, LEPR rs1137101 and PGC1A rs8192678 variants and transcription levels of genes of LEP signaling pathway (LEP, LEPR and PGC1A), antioxidant protection (SOD2) and inflammation (TNF-α), with the onset risk and clinical course (relapsing-remitting, RR and secondary progressive, SP) of MS. Genotyping of variants and determination of relative levels of target mRNAs in peripheral mononuclear leukocytes were performed by quantitative polymerase chain reaction (qPCR) using TaqMan® assays. Multiple logistic regression of three examined variants, by dominant model of inheritance, revealed an increased risk of MS in carriers of minor, A, allele of PGC1A variant. In men with MS, LEPR minor, G, allele was associated with higher values of the clinical parameter MSSS, compared to men with genotype AA, suggesting a potential sex-dependent effect of LEPR variant on disease severity. LEP mRNA was increased, while LEPR and PGC1A mRNA levels were decreased in patients with RR MS, compared to controls, indicating the association of transcription changes of these genes with the development of RR MS. LEP minor, A, allele may contribute to the determined increase in LEP mRNA levels in patients. Gene activity may be linked with disease severity, as LEPR mRNA and MSSS were shown to correlate. The results indicate that variants and changes in transcription levels of LEP, LEPR and PGC1A genes may affect the onset and severity of MS.Key words: multiple sclerosis, gene, variant, transcription, LEP, LEPR, PGC1A, SOD2, TNF-α",
publisher = "Универзитет у Београду, Биолошки факултет",
journal = "Универзитет у Београду",
title = "Studija asocijacije varijanti i nivoa transkripcije gena leptinskog signalnog puta (LEP, LEPR i PGC1A), antioksidativne zaštite (SOD2) i inflamacije (TNF-α) sa rizikom za nastanak i kliničkim tokom multiple skleroze",
url = "https://hdl.handle.net/21.15107/rcub_nardus_18742"
}

Kolić, I.. (2021). Studija asocijacije varijanti i nivoa transkripcije gena leptinskog signalnog puta (LEP, LEPR i PGC1A), antioksidativne zaštite (SOD2) i inflamacije (TNF-α) sa rizikom za nastanak i kliničkim tokom multiple skleroze. in Универзитет у Београду
Универзитет у Београду, Биолошки факултет..
https://hdl.handle.net/21.15107/rcub_nardus_18742

Kolić I. Studija asocijacije varijanti i nivoa transkripcije gena leptinskog signalnog puta (LEP, LEPR i PGC1A), antioksidativne zaštite (SOD2) i inflamacije (TNF-α) sa rizikom za nastanak i kliničkim tokom multiple skleroze. in Универзитет у Београду. 2021;.
https://hdl.handle.net/21.15107/rcub_nardus_18742 .

Kolić, Ivana, "Studija asocijacije varijanti i nivoa transkripcije gena leptinskog signalnog puta (LEP, LEPR i PGC1A), antioksidativne zaštite (SOD2) i inflamacije (TNF-α) sa rizikom za nastanak i kliničkim tokom multiple skleroze" in Универзитет у Београду (2021),
https://hdl.handle.net/21.15107/rcub_nardus_18742 .

TY  - JOUR
AU  - Kolić, Ivana
AU  - Stojković, Ljiljana S.
AU  - Stanković, Aleksandra
AU  - Stefanović, Milan
AU  - Dinčić, Evica
AU  - Živković, Maja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9542
AB  - Background: Leptin (LEP), leptin receptor (LEPR) and peroxisome proliferator-activated receptor gamma co-activator 1-alpha (PGC1A) are involved in the pathogenesis of multiple sclerosis (MS) by affecting the inflammatory response and reactive oxygen species production. LEP rs7799039 and LEPR rs1137101 genetic variants modify the serum LEP levels and PGC1A rs8192678 alters the PGC1A activity. The study objective was to explore the associations of these variants with susceptibility to MS, disease course/clinical parameters and also with peripheral blood mononuclear cell expression of the target genes and plasma LEP concentrations, in the study subjects. Methods: The study groups included 528 patients with MS and 429 controls. TaqMan® assays were used for genotyping and gene expression quantification. The Chi-square, parametric and nonparametric tests and simple/multiple logistic regression were performed for the statistical analysis of data. Results: A multiple logistic regression model including all three investigated variants, applied to patients (RRMS + SPMS) and controls, showed that PGC1A rs8192678 minor allele had an increased risk for the occurrence of disease, with OR (95%CI) = 1,32 (1,01–1,73), P = 0,04. Between-effect of gender and LEPR variant on the multiple sclerosis severity score (MSSS) was identified (P = 0,005). In male patients (relapsing-remitting and secondary progressive), LEPR minor allele carriers had increased MSSS (GG + AG vs AA, median (minimum–maximum) = 5,38 (0,64–9,88) vs 4,27 (0,78–9,63); P = 0,01, Padj = 0,03). In relapsing-remitting patients LEP rs7799039 affected the LEP gene expression (P = 0,006; Padj = 0,04). Conclusion: The current findings implicate an impact of investigated genetic variants on the pathogenesis of MS. © 2021 Elsevier B.V.
T2  - Gene
T1  - Association study of rs7799039, rs1137101 and rs8192678 gene variants with disease susceptibility/severity and corresponding LEP, LEPR and PGC1A gene expression in multiple sclerosis
VL  - 774
SP  - 145422
DO  - 10.1016/j.gene.2021.145422
ER  - 

@article{
author = "Kolić, Ivana and Stojković, Ljiljana S. and Stanković, Aleksandra and Stefanović, Milan and Dinčić, Evica and Živković, Maja",
year = "2021",
abstract = "Background: Leptin (LEP), leptin receptor (LEPR) and peroxisome proliferator-activated receptor gamma co-activator 1-alpha (PGC1A) are involved in the pathogenesis of multiple sclerosis (MS) by affecting the inflammatory response and reactive oxygen species production. LEP rs7799039 and LEPR rs1137101 genetic variants modify the serum LEP levels and PGC1A rs8192678 alters the PGC1A activity. The study objective was to explore the associations of these variants with susceptibility to MS, disease course/clinical parameters and also with peripheral blood mononuclear cell expression of the target genes and plasma LEP concentrations, in the study subjects. Methods: The study groups included 528 patients with MS and 429 controls. TaqMan® assays were used for genotyping and gene expression quantification. The Chi-square, parametric and nonparametric tests and simple/multiple logistic regression were performed for the statistical analysis of data. Results: A multiple logistic regression model including all three investigated variants, applied to patients (RRMS + SPMS) and controls, showed that PGC1A rs8192678 minor allele had an increased risk for the occurrence of disease, with OR (95%CI) = 1,32 (1,01–1,73), P = 0,04. Between-effect of gender and LEPR variant on the multiple sclerosis severity score (MSSS) was identified (P = 0,005). In male patients (relapsing-remitting and secondary progressive), LEPR minor allele carriers had increased MSSS (GG + AG vs AA, median (minimum–maximum) = 5,38 (0,64–9,88) vs 4,27 (0,78–9,63); P = 0,01, Padj = 0,03). In relapsing-remitting patients LEP rs7799039 affected the LEP gene expression (P = 0,006; Padj = 0,04). Conclusion: The current findings implicate an impact of investigated genetic variants on the pathogenesis of MS. © 2021 Elsevier B.V.",
journal = "Gene",
title = "Association study of rs7799039, rs1137101 and rs8192678 gene variants with disease susceptibility/severity and corresponding LEP, LEPR and PGC1A gene expression in multiple sclerosis",
volume = "774",
pages = "145422",
doi = "10.1016/j.gene.2021.145422"
}

Kolić, I., Stojković, L. S., Stanković, A., Stefanović, M., Dinčić, E.,& Živković, M.. (2021). Association study of rs7799039, rs1137101 and rs8192678 gene variants with disease susceptibility/severity and corresponding LEP, LEPR and PGC1A gene expression in multiple sclerosis. in Gene, 774, 145422.
https://doi.org/10.1016/j.gene.2021.145422

Kolić I, Stojković LS, Stanković A, Stefanović M, Dinčić E, Živković M. Association study of rs7799039, rs1137101 and rs8192678 gene variants with disease susceptibility/severity and corresponding LEP, LEPR and PGC1A gene expression in multiple sclerosis. in Gene. 2021;774:145422.
doi:10.1016/j.gene.2021.145422 .

Kolić, Ivana, Stojković, Ljiljana S., Stanković, Aleksandra, Stefanović, Milan, Dinčić, Evica, Živković, Maja, "Association study of rs7799039, rs1137101 and rs8192678 gene variants with disease susceptibility/severity and corresponding LEP, LEPR and PGC1A gene expression in multiple sclerosis" in Gene, 774 (2021):145422,
https://doi.org/10.1016/j.gene.2021.145422 . .

TY  - JOUR
AU  - Stojković, Ljiljana S.
AU  - Jovanović, Ivan G.
AU  - Živković, Maja
AU  - Zec, Manja
AU  - Đurić, Tamara
AU  - Životić, Ivan
AU  - Kuveljić, Jovana
AU  - Kolaković, Ana
AU  - Kolić, Ivana
AU  - Đorđević, Ana
AU  - Glibetić, Marija
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8683
AB  - Foods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of Aronia melanocarpa juice (AMJ), high-polyphenol (AMJ treatment, 1.17 g/100 mL polyphenols) and low-polyphenol (dAMJ treatment, 0.29 g/100 mL polyphenols) dose, on the transcriptome in peripheral blood mononuclear cells (PBMC) of 19 subjects at cardiovascular risk. Transcriptome data were obtained by microarray. Bioinformatic functional annotation analysis was performed on both the whole transcriptome datasets and the differentially expressed genes (DEGs). Expression of selected DEGs was validated by RT-qPCR. Administration of AMJ and dAMJ treatments during the two consecutive four-week treatment periods had additive effects on PBMC transcriptome profiles, with the most pronounced and specific effect noticed for AMJ in the last treatment period (TP3) of the trial. Between the high-dose and low-dose treatments in TP3, there was a multitude of overlapping DEGs and DEG-enriched biological processes and pathways, which primarily included immunomodulation and regulation of cell proliferation/death. Increased expression of TNF, IL1B, IL8, RGS1, OSM, and DUSP2 in TP3 was confirmed by RT-qPCR. The results suggest the immunomodulatory effects of prolonged habitual consumption of polyphenol-rich aronia juice in individuals at cardiovascular risk.
T2  - Nutrients
T1  - The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk
VL  - 12
IS  - 5
SP  - 1484
DO  - 10.3390/nu12051484
ER  - 

@article{
author = "Stojković, Ljiljana S. and Jovanović, Ivan G. and Živković, Maja and Zec, Manja and Đurić, Tamara and Životić, Ivan and Kuveljić, Jovana and Kolaković, Ana and Kolić, Ivana and Đorđević, Ana and Glibetić, Marija and Alavantić, Dragan and Stanković, Aleksandra",
year = "2020",
abstract = "Foods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of Aronia melanocarpa juice (AMJ), high-polyphenol (AMJ treatment, 1.17 g/100 mL polyphenols) and low-polyphenol (dAMJ treatment, 0.29 g/100 mL polyphenols) dose, on the transcriptome in peripheral blood mononuclear cells (PBMC) of 19 subjects at cardiovascular risk. Transcriptome data were obtained by microarray. Bioinformatic functional annotation analysis was performed on both the whole transcriptome datasets and the differentially expressed genes (DEGs). Expression of selected DEGs was validated by RT-qPCR. Administration of AMJ and dAMJ treatments during the two consecutive four-week treatment periods had additive effects on PBMC transcriptome profiles, with the most pronounced and specific effect noticed for AMJ in the last treatment period (TP3) of the trial. Between the high-dose and low-dose treatments in TP3, there was a multitude of overlapping DEGs and DEG-enriched biological processes and pathways, which primarily included immunomodulation and regulation of cell proliferation/death. Increased expression of TNF, IL1B, IL8, RGS1, OSM, and DUSP2 in TP3 was confirmed by RT-qPCR. The results suggest the immunomodulatory effects of prolonged habitual consumption of polyphenol-rich aronia juice in individuals at cardiovascular risk.",
journal = "Nutrients",
title = "The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk",
volume = "12",
number = "5",
pages = "1484",
doi = "10.3390/nu12051484"
}

Stojković, L. S., Jovanović, I. G., Živković, M., Zec, M., Đurić, T., Životić, I., Kuveljić, J., Kolaković, A., Kolić, I., Đorđević, A., Glibetić, M., Alavantić, D.,& Stanković, A.. (2020). The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk. in Nutrients, 12(5), 1484.
https://doi.org/10.3390/nu12051484

Stojković LS, Jovanović IG, Živković M, Zec M, Đurić T, Životić I, Kuveljić J, Kolaković A, Kolić I, Đorđević A, Glibetić M, Alavantić D, Stanković A. The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk. in Nutrients. 2020;12(5):1484.
doi:10.3390/nu12051484 .

Stojković, Ljiljana S., Jovanović, Ivan G., Živković, Maja, Zec, Manja, Đurić, Tamara, Životić, Ivan, Kuveljić, Jovana, Kolaković, Ana, Kolić, Ivana, Đorđević, Ana, Glibetić, Marija, Alavantić, Dragan, Stanković, Aleksandra, "The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk" in Nutrients, 12, no. 5 (2020):1484,
https://doi.org/10.3390/nu12051484 . .

TY  - JOUR
AU  - Kolić, Ivana
AU  - Stojković, Ljiljana S.
AU  - Dinčić, Evica
AU  - Jovanović, Ivan G.
AU  - Stanković, Aleksandra
AU  - Živković, Maja
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8618
AB  - Leptin (LEP) may contribute to the pathogenesis of multiple sclerosis (MS) by its immunomodulatory, proinflammatory and prooxidant effects. Therefore, plasma LEP levels and mRNA expression of five genes related to the LEP signaling pathway (LEP, LEP receptor (LEPR), peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC1A), superoxide dismutase 2, tumor necrosis factor-alpha) were investigated in relapsing-remitting MS. In patients (N = 64), compared to healthy subjects (N = 62), relative LEP mRNA levels were significantly increased (p = 0,01), while LEPR and PGC1A mRNA levels were decreased (p = 0,001 and p = 0,04, respectively). Significant positive correlation was observed between LEPR mRNA levels and clinical parameters of MS progression (EDSS, MSSS). © 2019
T2  - Journal of Neuroimmunology
T1  - Expression of LEP, LEPR and PGC1A genes is altered in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis
VL  - 338
SP  - 577090
DO  - 10.1016/j.jneuroim.2019.577090
ER  - 

@article{
author = "Kolić, Ivana and Stojković, Ljiljana S. and Dinčić, Evica and Jovanović, Ivan G. and Stanković, Aleksandra and Živković, Maja",
year = "2020",
abstract = "Leptin (LEP) may contribute to the pathogenesis of multiple sclerosis (MS) by its immunomodulatory, proinflammatory and prooxidant effects. Therefore, plasma LEP levels and mRNA expression of five genes related to the LEP signaling pathway (LEP, LEP receptor (LEPR), peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC1A), superoxide dismutase 2, tumor necrosis factor-alpha) were investigated in relapsing-remitting MS. In patients (N = 64), compared to healthy subjects (N = 62), relative LEP mRNA levels were significantly increased (p = 0,01), while LEPR and PGC1A mRNA levels were decreased (p = 0,001 and p = 0,04, respectively). Significant positive correlation was observed between LEPR mRNA levels and clinical parameters of MS progression (EDSS, MSSS). © 2019",
journal = "Journal of Neuroimmunology",
title = "Expression of LEP, LEPR and PGC1A genes is altered in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis",
volume = "338",
pages = "577090",
doi = "10.1016/j.jneuroim.2019.577090"
}

Kolić, I., Stojković, L. S., Dinčić, E., Jovanović, I. G., Stanković, A.,& Živković, M.. (2020). Expression of LEP, LEPR and PGC1A genes is altered in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis. in Journal of Neuroimmunology, 338, 577090.
https://doi.org/10.1016/j.jneuroim.2019.577090

Kolić I, Stojković LS, Dinčić E, Jovanović IG, Stanković A, Živković M. Expression of LEP, LEPR and PGC1A genes is altered in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis. in Journal of Neuroimmunology. 2020;338:577090.
doi:10.1016/j.jneuroim.2019.577090 .

Kolić, Ivana, Stojković, Ljiljana S., Dinčić, Evica, Jovanović, Ivan G., Stanković, Aleksandra, Živković, Maja, "Expression of LEP, LEPR and PGC1A genes is altered in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis" in Journal of Neuroimmunology, 338 (2020):577090,
https://doi.org/10.1016/j.jneuroim.2019.577090 . .

TY  - CONF
AU  - Životić, Ivan
AU  - Kolić, Ivana
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Milovanović, Branislav
AU  - Stanković, Aleksandra
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12682
AB  - Introduction: The autonomic nervous system (ANS), plays an essential role in the regulation of vascular wall contractility, heart rate and blood pressure (BP) variability. The symptoms of ANS dysfunction were strongly associated with vasovagal syncope (VVS). The renin-angiotensin system (RAS) is a powerful feedback system responsible for long-term control of cardiovascular hemodynamic values, heart rate and BP homeostasis. All components of the RAS have been detected in the brain as well as in the cardiovascular system, with central and peripheral actions of angiotensin II main effector molecule synthesized by angiotensin converting enzyme (ACE). Opposite influences of angiotensin II (AngII) receptor type 1 (AT1R) and angiotensin II receptor type 2 (ATR2) on sympathetic tone have been documented. Genetic studies have shown that individuals with VVS usually have a positive family history. Still, the genetic basis of VVS is unclear. The aim of the study was to investigate the association of the RAS gene variants, ACE I/D, AT1R A1166C and ATR2 -1332 A/G with the VVS in Serbian population. Methods: This case-control designed study included 215 VVS patients and 439 controls (C) matched by age (mean±SD, VVS: 38,25±13,52 years and C: 36,63±11,69 years). Genotyping was done by PCR-RFLP and allele specific PCR methods. Results: There were no significant differences in the genotype frequency distributions of ACE I/D and AT1R A1166C variants between cases and controls (p=0.4 and p=0.2, respectively). With regard to X-linked ATR2 -1332 A/G variant the frequency of A/- hemizygotes was significantly higher in VVS than in controls, in males (p=0.01). In females there were no differences in genotype frequency distributions between cases and controls (p=0.73). Conclusions: Our results suggest further investigation of AT2R hemodynamic effect on AngII in VVS males. To accurately elucidate given association, replication of the results in a larger sample is inevitable.
C3  - ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts
T1  - Renin-angiotensin system gene variants in association with vasovagal syncope in Serbian population
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12682
ER  - 

@conference{
author = "Životić, Ivan and Kolić, Ivana and Đurić, Tamara and Živković, Maja and Milovanović, Branislav and Stanković, Aleksandra",
year = "2019",
abstract = "Introduction: The autonomic nervous system (ANS), plays an essential role in the regulation of vascular wall contractility, heart rate and blood pressure (BP) variability. The symptoms of ANS dysfunction were strongly associated with vasovagal syncope (VVS). The renin-angiotensin system (RAS) is a powerful feedback system responsible for long-term control of cardiovascular hemodynamic values, heart rate and BP homeostasis. All components of the RAS have been detected in the brain as well as in the cardiovascular system, with central and peripheral actions of angiotensin II main effector molecule synthesized by angiotensin converting enzyme (ACE). Opposite influences of angiotensin II (AngII) receptor type 1 (AT1R) and angiotensin II receptor type 2 (ATR2) on sympathetic tone have been documented. Genetic studies have shown that individuals with VVS usually have a positive family history. Still, the genetic basis of VVS is unclear. The aim of the study was to investigate the association of the RAS gene variants, ACE I/D, AT1R A1166C and ATR2 -1332 A/G with the VVS in Serbian population. Methods: This case-control designed study included 215 VVS patients and 439 controls (C) matched by age (mean±SD, VVS: 38,25±13,52 years and C: 36,63±11,69 years). Genotyping was done by PCR-RFLP and allele specific PCR methods. Results: There were no significant differences in the genotype frequency distributions of ACE I/D and AT1R A1166C variants between cases and controls (p=0.4 and p=0.2, respectively). With regard to X-linked ATR2 -1332 A/G variant the frequency of A/- hemizygotes was significantly higher in VVS than in controls, in males (p=0.01). In females there were no differences in genotype frequency distributions between cases and controls (p=0.73). Conclusions: Our results suggest further investigation of AT2R hemodynamic effect on AngII in VVS males. To accurately elucidate given association, replication of the results in a larger sample is inevitable.",
journal = "ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts",
title = "Renin-angiotensin system gene variants in association with vasovagal syncope in Serbian population",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12682"
}

Životić, I., Kolić, I., Đurić, T., Živković, M., Milovanović, B.,& Stanković, A.. (2019). Renin-angiotensin system gene variants in association with vasovagal syncope in Serbian population. in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts.
https://hdl.handle.net/21.15107/rcub_vinar_12682

Životić I, Kolić I, Đurić T, Živković M, Milovanović B, Stanković A. Renin-angiotensin system gene variants in association with vasovagal syncope in Serbian population. in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts. 2019;.
https://hdl.handle.net/21.15107/rcub_vinar_12682 .

Životić, Ivan, Kolić, Ivana, Đurić, Tamara, Živković, Maja, Milovanović, Branislav, Stanković, Aleksandra, "Renin-angiotensin system gene variants in association with vasovagal syncope in Serbian population" in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts (2019),
https://hdl.handle.net/21.15107/rcub_vinar_12682 .

TY  - CONF
AU  - Kolić, Ivana
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Alavantić, Dragan
AU  - Jovanović, Dušica
AU  - Milovanović, Branislav
AU  - Stanković, Aleksandra
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12683
AB  - Background: Disturbance in sympathovagal balance were strongly associated withoccurrence of syncope.The renin-angiotensin system (RAS) interacts with the autonomous nervous system (ANS) in the regulation of blood pressure and cardiovascular function. By now, several genetic variants in the RAS have been identified as factors in alteration of HRV parameters, haemodynamic values,heart rate, and BP.The aim of our study was to investigate the association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants and ANS function. Methods: This study included 215 syncope patients (age (mean±SD)=38.15±13.52 years, 79% females and 21% males) whose ANS function was evaluated by power spectral analysis of heart rate variability (HRV) before and during Tilt test (TT). Genotyping was done by PCR-RFLP and allele specific PCR methods. Statistical analysis was performed using STATISTICA data analysis software system (StatSoft, Inc. (2007). STATISTICA, version 8.0. www.statsoft.com). Results: There were no significant associations of ACE I/D or AGTR2 -1332A/G gene variants with the level of HRV parameters. We found that homozygotic carriers of both AGTR1 +1166A/C alleles have significantly increased LF component in supine position before TT, compared to heterozygote carriers (p=0,04, Mann-Whitney U test). During the TT there were no significant diferences in level of LF component with regard to AGTR1 +1166A/C genotypes. Conclusions: The present study suggest association of AGTR1 +1166A/C variant with LF component adrressing predisposition to syncopal event during TT. This association need to be confirmed in further genetic association studies on a larger sample.
C3  - ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts
T1  - Association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants with autonomous nervous system function in Serbian syncope patients
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12683
ER  - 

@conference{
author = "Kolić, Ivana and Životić, Ivan and Đurić, Tamara and Živković, Maja and Alavantić, Dragan and Jovanović, Dušica and Milovanović, Branislav and Stanković, Aleksandra",
year = "2019",
abstract = "Background: Disturbance in sympathovagal balance were strongly associated withoccurrence of syncope.The renin-angiotensin system (RAS) interacts with the autonomous nervous system (ANS) in the regulation of blood pressure and cardiovascular function. By now, several genetic variants in the RAS have been identified as factors in alteration of HRV parameters, haemodynamic values,heart rate, and BP.The aim of our study was to investigate the association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants and ANS function. Methods: This study included 215 syncope patients (age (mean±SD)=38.15±13.52 years, 79% females and 21% males) whose ANS function was evaluated by power spectral analysis of heart rate variability (HRV) before and during Tilt test (TT). Genotyping was done by PCR-RFLP and allele specific PCR methods. Statistical analysis was performed using STATISTICA data analysis software system (StatSoft, Inc. (2007). STATISTICA, version 8.0. www.statsoft.com). Results: There were no significant associations of ACE I/D or AGTR2 -1332A/G gene variants with the level of HRV parameters. We found that homozygotic carriers of both AGTR1 +1166A/C alleles have significantly increased LF component in supine position before TT, compared to heterozygote carriers (p=0,04, Mann-Whitney U test). During the TT there were no significant diferences in level of LF component with regard to AGTR1 +1166A/C genotypes. Conclusions: The present study suggest association of AGTR1 +1166A/C variant with LF component adrressing predisposition to syncopal event during TT. This association need to be confirmed in further genetic association studies on a larger sample.",
journal = "ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts",
title = "Association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants with autonomous nervous system function in Serbian syncope patients",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12683"
}

Kolić, I., Životić, I., Đurić, T., Živković, M., Alavantić, D., Jovanović, D., Milovanović, B.,& Stanković, A.. (2019). Association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants with autonomous nervous system function in Serbian syncope patients. in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts.
https://hdl.handle.net/21.15107/rcub_vinar_12683

Kolić I, Životić I, Đurić T, Živković M, Alavantić D, Jovanović D, Milovanović B, Stanković A. Association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants with autonomous nervous system function in Serbian syncope patients. in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts. 2019;.
https://hdl.handle.net/21.15107/rcub_vinar_12683 .

Kolić, Ivana, Životić, Ivan, Đurić, Tamara, Živković, Maja, Alavantić, Dragan, Jovanović, Dušica, Milovanović, Branislav, Stanković, Aleksandra, "Association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants with autonomous nervous system function in Serbian syncope patients" in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts (2019),
https://hdl.handle.net/21.15107/rcub_vinar_12683 .

TY  - JOUR
AU  - Živković, Maja
AU  - Kolić, Ivana
AU  - Ješić, Snežana
AU  - Jotić, Ana
AU  - Stanković, Aleksandra
PY  - 2018
UR  - http://e-ceo.org/journal/view.php?doi=10.21053/ceo.2017.01060
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7677
AB  - Objectives. Chronic otitis media (COM) is followed by irreversible tissue damage and destruction of the middle ear structures, with the possibility of complications under the maintenance of inflammation. Inflammatory mediators such as cytokines play a crucial role in the initial stage of inflammation. The aim of this study was to evaluate the association of the polymorphisms in two innate immunity/inflammation cascade genes from interleukin-1 (IL-1) gene cluster with COM with regard to cholesteatoma. Methods. In the cross-sectional case-control study, DNA samples were collected from 189 patients with COM and 119 controls from a population of Serbia. The +3953 C/T (rs1143634), TaqI polymorphism in interleukin-1 beta (IL-1Β) gene and 86 bp variable number tandem repeat (VNTR, rs2234663) polymorphism in the IL-1 receptor antagonist (IL-1RA) gene were analyzed by polymerase chain reaction. Results. The IL-1Β TaqI polymorphism was not significantly different in patients compared with the control group. The significant difference between patients and controls was observed for both, genotype and allele frequencies of IL-1RA VNTR polymorphism (chi-square P<0.01). We found that carriers of IL-1RA allele 2 (odds ratio, 0.47; 95% confidence interval, 0.29 to 0.76; P=0.004) have a favorable association with COM, using multivariate logistic analysis that included both polymorphisms, age and sex. The IL-1RA allele frequency distribution was significantly different with regard to cholesteatoma. Conclusion. The carriers of allele 2 of VNTR IL-1RA polymorphism had a decreased odds ratio for COM, which is in agreement with findings in other inflammatory disease and its previous association with higher IL-1RA levels. Possible down-regulation of IL-1 mediated proinflammatory signaling pathways via IL-1RA in COM as well as results of our study should be further investigated and replicated.
T2  - Clinical and Experimental Otorhinolaryngology
T1  - The Allele 2 of the VNTR Polymorphism in the Gene That Encodes a Natural Inhibitor of IL-1β, IL-1RA Is Favorably Associated With Chronic Otitis Media
VL  - 11
IS  - 2
SP  - 118
EP  - 123
DO  - 10.21053/ceo.2017.01060
ER  - 

@article{
author = "Živković, Maja and Kolić, Ivana and Ješić, Snežana and Jotić, Ana and Stanković, Aleksandra",
year = "2018",
abstract = "Objectives. Chronic otitis media (COM) is followed by irreversible tissue damage and destruction of the middle ear structures, with the possibility of complications under the maintenance of inflammation. Inflammatory mediators such as cytokines play a crucial role in the initial stage of inflammation. The aim of this study was to evaluate the association of the polymorphisms in two innate immunity/inflammation cascade genes from interleukin-1 (IL-1) gene cluster with COM with regard to cholesteatoma. Methods. In the cross-sectional case-control study, DNA samples were collected from 189 patients with COM and 119 controls from a population of Serbia. The +3953 C/T (rs1143634), TaqI polymorphism in interleukin-1 beta (IL-1Β) gene and 86 bp variable number tandem repeat (VNTR, rs2234663) polymorphism in the IL-1 receptor antagonist (IL-1RA) gene were analyzed by polymerase chain reaction. Results. The IL-1Β TaqI polymorphism was not significantly different in patients compared with the control group. The significant difference between patients and controls was observed for both, genotype and allele frequencies of IL-1RA VNTR polymorphism (chi-square P<0.01). We found that carriers of IL-1RA allele 2 (odds ratio, 0.47; 95% confidence interval, 0.29 to 0.76; P=0.004) have a favorable association with COM, using multivariate logistic analysis that included both polymorphisms, age and sex. The IL-1RA allele frequency distribution was significantly different with regard to cholesteatoma. Conclusion. The carriers of allele 2 of VNTR IL-1RA polymorphism had a decreased odds ratio for COM, which is in agreement with findings in other inflammatory disease and its previous association with higher IL-1RA levels. Possible down-regulation of IL-1 mediated proinflammatory signaling pathways via IL-1RA in COM as well as results of our study should be further investigated and replicated.",
journal = "Clinical and Experimental Otorhinolaryngology",
title = "The Allele 2 of the VNTR Polymorphism in the Gene That Encodes a Natural Inhibitor of IL-1β, IL-1RA Is Favorably Associated With Chronic Otitis Media",
volume = "11",
number = "2",
pages = "118-123",
doi = "10.21053/ceo.2017.01060"
}

Živković, M., Kolić, I., Ješić, S., Jotić, A.,& Stanković, A.. (2018). The Allele 2 of the VNTR Polymorphism in the Gene That Encodes a Natural Inhibitor of IL-1β, IL-1RA Is Favorably Associated With Chronic Otitis Media. in Clinical and Experimental Otorhinolaryngology, 11(2), 118-123.
https://doi.org/10.21053/ceo.2017.01060

Živković M, Kolić I, Ješić S, Jotić A, Stanković A. The Allele 2 of the VNTR Polymorphism in the Gene That Encodes a Natural Inhibitor of IL-1β, IL-1RA Is Favorably Associated With Chronic Otitis Media. in Clinical and Experimental Otorhinolaryngology. 2018;11(2):118-123.
doi:10.21053/ceo.2017.01060 .

Živković, Maja, Kolić, Ivana, Ješić, Snežana, Jotić, Ana, Stanković, Aleksandra, "The Allele 2 of the VNTR Polymorphism in the Gene That Encodes a Natural Inhibitor of IL-1β, IL-1RA Is Favorably Associated With Chronic Otitis Media" in Clinical and Experimental Otorhinolaryngology, 11, no. 2 (2018):118-123,
https://doi.org/10.21053/ceo.2017.01060 . .

TY  - JOUR
AU  - Kostic, Smiljana
AU  - Kolić, Ivana
AU  - Raičević, Ranko
AU  - Stojanovic, Zvezdana
AU  - Kostić, Dejan
AU  - Dinčić, Evica
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1626
AB  - Background/Aim. Due to the fact that there is a relatively small number of data related to systemic insulin abnormalities in the multiple sclerosis (MS), the main objective of our study was to determine whether a dysbalance of glucose and insulin metabolism exist in patients with natural course of MS. Our hypothesis was that the metabolic disorder that characterizes state of the insulin resistance (IR) and reduced insulin sensitivity (IS) in untreated patients with MS could play a role in disease progression and degree of functional disability. Methods. The study included 31 patients with relapsing-remitting (RR) MS and 14 healthy controls from the same geographic area matched by age, ethnicity and number of smokers. The glucose tolerance, IS, and IR were examined using an oral glucose tolerance test (OGTT) and using basal plasma glucose and insulin levels. The functional disability and disease progression were evaluated by the Expanded Disability Status Scale (EDSS) and Multiple Sclerosis Severity Score (MSSS). Results. The MS patients tolerated glucose equally well as the healthy controls. Basal concentrations of insulin were significantly higher in the MS group p LT 0.05), as well as insulin plasma level 30 min after oral glucose load (p LT 0.01). The patients with MS had significantly higher values of homeostasis model assessment indexes of IR (HOMA-IR) (p = 0.027; p = 0.028). The percentage of IS (HOMA2 % S) and whole body IS index (ISI Matsuda) showed significantly lower values in the MS patients than in the controls (p = 0.005; p = 0.001). The insulinogenic index in the first 30 min of OGTT was significantly higher in MS patients (p = 0.005). The measures of functional disability and MS progression did not correlate significantly with the investigated parameters of IR and IS indexes. Conclusion. This study demonstrates for the first time the existence of hyperinsulinemia, reduced insulin sensitivity and normal glucose tolerance that indicate the initial phase of IR in the natural course of MS. Additional research is necessary in order to define the mechanisms of occurrence and the impact of IR on the complex pathophysiological processes in MS.
T2  - Vojnosanitetski pregled
T1  - Insulin resistance in drug naive patients with multiple sclerosis
VL  - 74
IS  - 6
SP  - 563
EP  - 570
DO  - 10.2298/VSP160218082K
ER  - 

@article{
author = "Kostic, Smiljana and Kolić, Ivana and Raičević, Ranko and Stojanovic, Zvezdana and Kostić, Dejan and Dinčić, Evica",
year = "2017",
abstract = "Background/Aim. Due to the fact that there is a relatively small number of data related to systemic insulin abnormalities in the multiple sclerosis (MS), the main objective of our study was to determine whether a dysbalance of glucose and insulin metabolism exist in patients with natural course of MS. Our hypothesis was that the metabolic disorder that characterizes state of the insulin resistance (IR) and reduced insulin sensitivity (IS) in untreated patients with MS could play a role in disease progression and degree of functional disability. Methods. The study included 31 patients with relapsing-remitting (RR) MS and 14 healthy controls from the same geographic area matched by age, ethnicity and number of smokers. The glucose tolerance, IS, and IR were examined using an oral glucose tolerance test (OGTT) and using basal plasma glucose and insulin levels. The functional disability and disease progression were evaluated by the Expanded Disability Status Scale (EDSS) and Multiple Sclerosis Severity Score (MSSS). Results. The MS patients tolerated glucose equally well as the healthy controls. Basal concentrations of insulin were significantly higher in the MS group p LT 0.05), as well as insulin plasma level 30 min after oral glucose load (p LT 0.01). The patients with MS had significantly higher values of homeostasis model assessment indexes of IR (HOMA-IR) (p = 0.027; p = 0.028). The percentage of IS (HOMA2 % S) and whole body IS index (ISI Matsuda) showed significantly lower values in the MS patients than in the controls (p = 0.005; p = 0.001). The insulinogenic index in the first 30 min of OGTT was significantly higher in MS patients (p = 0.005). The measures of functional disability and MS progression did not correlate significantly with the investigated parameters of IR and IS indexes. Conclusion. This study demonstrates for the first time the existence of hyperinsulinemia, reduced insulin sensitivity and normal glucose tolerance that indicate the initial phase of IR in the natural course of MS. Additional research is necessary in order to define the mechanisms of occurrence and the impact of IR on the complex pathophysiological processes in MS.",
journal = "Vojnosanitetski pregled",
title = "Insulin resistance in drug naive patients with multiple sclerosis",
volume = "74",
number = "6",
pages = "563-570",
doi = "10.2298/VSP160218082K"
}

Kostic, S., Kolić, I., Raičević, R., Stojanovic, Z., Kostić, D.,& Dinčić, E.. (2017). Insulin resistance in drug naive patients with multiple sclerosis. in Vojnosanitetski pregled, 74(6), 563-570.
https://doi.org/10.2298/VSP160218082K

Kostic S, Kolić I, Raičević R, Stojanovic Z, Kostić D, Dinčić E. Insulin resistance in drug naive patients with multiple sclerosis. in Vojnosanitetski pregled. 2017;74(6):563-570.
doi:10.2298/VSP160218082K .

Kostic, Smiljana, Kolić, Ivana, Raičević, Ranko, Stojanovic, Zvezdana, Kostić, Dejan, Dinčić, Evica, "Insulin resistance in drug naive patients with multiple sclerosis" in Vojnosanitetski pregled, 74, no. 6 (2017):563-570,
https://doi.org/10.2298/VSP160218082K . .

TY  - JOUR
AU  - Jovanović, Ivan G.
AU  - Živković, Maja
AU  - Kostić, Mirjana M.
AU  - Krstić, Zoran
AU  - Đurić, Tamara
AU  - Kolić, Ivana
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1182
AB  - Background: The genetic cause of most congenital anomalies of the kidney and urinary tract (CAKUT) cases remains unknown, therefore the novel approaches in searching for the common disease denominators are required. miRs regulate gene expression in humans and therefore have potentially therapeutic and biomarker properties. No studies thus far have attempted to explore the miRs in human CAKUT. We applied a new strategy to identify most specific miRs associated with CAKUT, in pediatric patients. Methods: Data from the whole genome expression, gathered from ureter tissue samples of 19 patients and 7 controls, were used for the bioinformatic prediction of miRs activity in CAKUT. We integrated microarray gene expression data and miR target predictions from multiple prediction algorithms using Co-inertia analysis (CIA) in conjunction with correspondence analysis and between group analysis, to produce a ranked list of miRs associated with CAKUT. The CIA included five different sequence based miR target prediction algorithms and the Co-expression Meta-analysis of miR Targets. For the experimental validation of expression of miRs identified by the CIA we used tissue from 36 CAKUT patients and 9 controls. The results of gene ontology (GO) analysis on co-expressed targets of miRs associated with CAKUT were used for the selection of putative biological processes relevant to CAKUT. Results: We identified 7 miRs with a potential role in CAKUT. The top ranked miRs from miRCos communities 4, 1 and 7 were chosen for experimental validation of expression in CAKUT tissue. The 5.7 fold increase of hsa-miR-144 expression in human tissue from CAKUT patients compared to controls (p = 0.005) was observed. From the GO we selected 7 biological processes that could contribute to CAKUT, which genes are potentially influenced by hsa-miR-144. The hsa-miR-200a, hsa-miR-183 and hsa-miR-375 werent differentially expressed in CAKUT. Conclusions: This study shows that integrative approach applied here was useful in identification of the miRs associated with CAKUT. The hsa-miR-144, first time identified in CAKUT, could be connected with biological processes crucial for normal development of kidney and urinary tract. Further functional analysis must follow to reveal the impact of hsa-miR-144 on CAKUT occurrence.
T2  - Journal of Translational Medicine
T1  - Transcriptome-wide based identification of miRs in congenital anomalies of the kidney and urinary tract (CAKUT) in children: the significant upregulation of tissue miR-144 expression
VL  - 14
IS  - 1
SP  - 193
DO  - 10.1186/s12967-016-0955-0
ER  - 

@article{
author = "Jovanović, Ivan G. and Živković, Maja and Kostić, Mirjana M. and Krstić, Zoran and Đurić, Tamara and Kolić, Ivana and Alavantić, Dragan and Stanković, Aleksandra",
year = "2016",
abstract = "Background: The genetic cause of most congenital anomalies of the kidney and urinary tract (CAKUT) cases remains unknown, therefore the novel approaches in searching for the common disease denominators are required. miRs regulate gene expression in humans and therefore have potentially therapeutic and biomarker properties. No studies thus far have attempted to explore the miRs in human CAKUT. We applied a new strategy to identify most specific miRs associated with CAKUT, in pediatric patients. Methods: Data from the whole genome expression, gathered from ureter tissue samples of 19 patients and 7 controls, were used for the bioinformatic prediction of miRs activity in CAKUT. We integrated microarray gene expression data and miR target predictions from multiple prediction algorithms using Co-inertia analysis (CIA) in conjunction with correspondence analysis and between group analysis, to produce a ranked list of miRs associated with CAKUT. The CIA included five different sequence based miR target prediction algorithms and the Co-expression Meta-analysis of miR Targets. For the experimental validation of expression of miRs identified by the CIA we used tissue from 36 CAKUT patients and 9 controls. The results of gene ontology (GO) analysis on co-expressed targets of miRs associated with CAKUT were used for the selection of putative biological processes relevant to CAKUT. Results: We identified 7 miRs with a potential role in CAKUT. The top ranked miRs from miRCos communities 4, 1 and 7 were chosen for experimental validation of expression in CAKUT tissue. The 5.7 fold increase of hsa-miR-144 expression in human tissue from CAKUT patients compared to controls (p = 0.005) was observed. From the GO we selected 7 biological processes that could contribute to CAKUT, which genes are potentially influenced by hsa-miR-144. The hsa-miR-200a, hsa-miR-183 and hsa-miR-375 werent differentially expressed in CAKUT. Conclusions: This study shows that integrative approach applied here was useful in identification of the miRs associated with CAKUT. The hsa-miR-144, first time identified in CAKUT, could be connected with biological processes crucial for normal development of kidney and urinary tract. Further functional analysis must follow to reveal the impact of hsa-miR-144 on CAKUT occurrence.",
journal = "Journal of Translational Medicine",
title = "Transcriptome-wide based identification of miRs in congenital anomalies of the kidney and urinary tract (CAKUT) in children: the significant upregulation of tissue miR-144 expression",
volume = "14",
number = "1",
pages = "193",
doi = "10.1186/s12967-016-0955-0"
}

Jovanović, I. G., Živković, M., Kostić, M. M., Krstić, Z., Đurić, T., Kolić, I., Alavantić, D.,& Stanković, A.. (2016). Transcriptome-wide based identification of miRs in congenital anomalies of the kidney and urinary tract (CAKUT) in children: the significant upregulation of tissue miR-144 expression. in Journal of Translational Medicine, 14(1), 193.
https://doi.org/10.1186/s12967-016-0955-0

Jovanović IG, Živković M, Kostić MM, Krstić Z, Đurić T, Kolić I, Alavantić D, Stanković A. Transcriptome-wide based identification of miRs in congenital anomalies of the kidney and urinary tract (CAKUT) in children: the significant upregulation of tissue miR-144 expression. in Journal of Translational Medicine. 2016;14(1):193.
doi:10.1186/s12967-016-0955-0 .

Jovanović, Ivan G., Živković, Maja, Kostić, Mirjana M., Krstić, Zoran, Đurić, Tamara, Kolić, Ivana, Alavantić, Dragan, Stanković, Aleksandra, "Transcriptome-wide based identification of miRs in congenital anomalies of the kidney and urinary tract (CAKUT) in children: the significant upregulation of tissue miR-144 expression" in Journal of Translational Medicine, 14, no. 1 (2016):193,
https://doi.org/10.1186/s12967-016-0955-0 . .

TY  - CONF
AU  - Jotić, Ana
AU  - Kuveljić, Jovana
AU  - Kolaković, Ana
AU  - Kolić, Ivana
AU  - Živković, Maja
AU  - Jesić, Snežana
AU  - Stanković, Aleksandra
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12387
AB  - Toll like receptors (TLRs) are the pattern recognition receptors, important for the innate immune system, the first line of defense against bacterial infections which are the main cause of otitis media (OM). Our aim was to investigate the association of TLR4 (Thr399Ile and Asp299Gly) and TLR2 (Arg753Gln) most common polymorphisms with OM prevalence as well as with morphologic changes of middle ear during chronic otitis media. We had 111 controls and 186 patients divided into three groups of COM: nonsuppurative OM, suppurative OM and cholesteatoma. Frequencies of the genotypes of the TLR 2 gene polymorphism were significantly different between men and women having nonsuppurative OM (p=0.03).
PB  - Belgrade : Serbian Genetic Society
C3  - V Congress of the Serbian Genetic Society : Book of abstracts
T1  - Toll like receptors 2 and 4 polymorphisms in chronic otitis media
SP  - 52
EP  - 52
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12387
ER  - 

@conference{
author = "Jotić, Ana and Kuveljić, Jovana and Kolaković, Ana and Kolić, Ivana and Živković, Maja and Jesić, Snežana and Stanković, Aleksandra",
year = "2014",
abstract = "Toll like receptors (TLRs) are the pattern recognition receptors, important for the innate immune system, the first line of defense against bacterial infections which are the main cause of otitis media (OM). Our aim was to investigate the association of TLR4 (Thr399Ile and Asp299Gly) and TLR2 (Arg753Gln) most common polymorphisms with OM prevalence as well as with morphologic changes of middle ear during chronic otitis media. We had 111 controls and 186 patients divided into three groups of COM: nonsuppurative OM, suppurative OM and cholesteatoma. Frequencies of the genotypes of the TLR 2 gene polymorphism were significantly different between men and women having nonsuppurative OM (p=0.03).",
publisher = "Belgrade : Serbian Genetic Society",
journal = "V Congress of the Serbian Genetic Society : Book of abstracts",
title = "Toll like receptors 2 and 4 polymorphisms in chronic otitis media",
pages = "52-52",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12387"
}

Jotić, A., Kuveljić, J., Kolaković, A., Kolić, I., Živković, M., Jesić, S.,& Stanković, A.. (2014). Toll like receptors 2 and 4 polymorphisms in chronic otitis media. in V Congress of the Serbian Genetic Society : Book of abstracts
Belgrade : Serbian Genetic Society., 52-52.
https://hdl.handle.net/21.15107/rcub_vinar_12387

Jotić A, Kuveljić J, Kolaković A, Kolić I, Živković M, Jesić S, Stanković A. Toll like receptors 2 and 4 polymorphisms in chronic otitis media. in V Congress of the Serbian Genetic Society : Book of abstracts. 2014;:52-52.
https://hdl.handle.net/21.15107/rcub_vinar_12387 .

Jotić, Ana, Kuveljić, Jovana, Kolaković, Ana, Kolić, Ivana, Živković, Maja, Jesić, Snežana, Stanković, Aleksandra, "Toll like receptors 2 and 4 polymorphisms in chronic otitis media" in V Congress of the Serbian Genetic Society : Book of abstracts (2014):52-52,
https://hdl.handle.net/21.15107/rcub_vinar_12387 .

TY  - CONF
AU  - Kolić, Ivana
AU  - Živković, Maja
AU  - Kuveljić, Jovana
AU  - Jesić, Snežana
AU  - Stanković, Aleksandra
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12388
AB  - Constitutional polymorphisms in the proinflamatory cytokine genes influence the individual cytokine secretion levels, the course and severity of inflammation. Aim of our study was to investigate the association of IL-1β +3953 C/T and IL1-Ra86 bp VNTR gene polymorphisms with the development of chronic otitis media (COM) in 144 patients and 103 controls. Frequencies of the alleles and genotypes of the IL-1Ra gene polymorphism were significantly different between the patients and controls (alleles: p=0.003; genotypes: p=0.0001). Non-carriers of IL-1Ra allele 2 had significatly higher risk for COM occurence (p=0.002; OR=2.98; ±95%CI, 1.68-5.26). This allele is a potential protective genetic marker for COM.
PB  - Belgrade : Serbian Genetic Society
C3  - V Congress of the Serbian Genetic Society : Book of abstracts
T1  - The IL-1β and IL-1Ra gene polymorphisms and chronic inflammation of the middle ear
SP  - 42
EP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12388
ER  - 

@conference{
author = "Kolić, Ivana and Živković, Maja and Kuveljić, Jovana and Jesić, Snežana and Stanković, Aleksandra",
year = "2014",
abstract = "Constitutional polymorphisms in the proinflamatory cytokine genes influence the individual cytokine secretion levels, the course and severity of inflammation. Aim of our study was to investigate the association of IL-1β +3953 C/T and IL1-Ra86 bp VNTR gene polymorphisms with the development of chronic otitis media (COM) in 144 patients and 103 controls. Frequencies of the alleles and genotypes of the IL-1Ra gene polymorphism were significantly different between the patients and controls (alleles: p=0.003; genotypes: p=0.0001). Non-carriers of IL-1Ra allele 2 had significatly higher risk for COM occurence (p=0.002; OR=2.98; ±95%CI, 1.68-5.26). This allele is a potential protective genetic marker for COM.",
publisher = "Belgrade : Serbian Genetic Society",
journal = "V Congress of the Serbian Genetic Society : Book of abstracts",
title = "The IL-1β and IL-1Ra gene polymorphisms and chronic inflammation of the middle ear",
pages = "42-42",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12388"
}

Kolić, I., Živković, M., Kuveljić, J., Jesić, S.,& Stanković, A.. (2014). The IL-1β and IL-1Ra gene polymorphisms and chronic inflammation of the middle ear. in V Congress of the Serbian Genetic Society : Book of abstracts
Belgrade : Serbian Genetic Society., 42-42.
https://hdl.handle.net/21.15107/rcub_vinar_12388

Kolić I, Živković M, Kuveljić J, Jesić S, Stanković A. The IL-1β and IL-1Ra gene polymorphisms and chronic inflammation of the middle ear. in V Congress of the Serbian Genetic Society : Book of abstracts. 2014;:42-42.
https://hdl.handle.net/21.15107/rcub_vinar_12388 .

Kolić, Ivana, Živković, Maja, Kuveljić, Jovana, Jesić, Snežana, Stanković, Aleksandra, "The IL-1β and IL-1Ra gene polymorphisms and chronic inflammation of the middle ear" in V Congress of the Serbian Genetic Society : Book of abstracts (2014):42-42,
https://hdl.handle.net/21.15107/rcub_vinar_12388 .

TY  - CONF
AU  - Stanković, Aleksandra
AU  - Kolić, Ivana
AU  - Živković, Maja
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12686
PB  - Belgrade : Serbian Neuroscience Society
C3  - NEUROCARD 2014 : 6th International Symposium on Neurocardiology : The 5th International Symposium on Noninvasive Electrocardiology : Book of abstracts
T1  - Genetic polymorphism SCN5A-H558R as potential target for future therapies of trafficking defect associated with cardiac sodium channel mutations: S216L, V1951L and R282H
SP  - 57
EP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12686
ER  - 

@conference{
author = "Stanković, Aleksandra and Kolić, Ivana and Živković, Maja",
year = "2014",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "NEUROCARD 2014 : 6th International Symposium on Neurocardiology : The 5th International Symposium on Noninvasive Electrocardiology : Book of abstracts",
title = "Genetic polymorphism SCN5A-H558R as potential target for future therapies of trafficking defect associated with cardiac sodium channel mutations: S216L, V1951L and R282H",
pages = "57-57",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12686"
}

Stanković, A., Kolić, I.,& Živković, M.. (2014). Genetic polymorphism SCN5A-H558R as potential target for future therapies of trafficking defect associated with cardiac sodium channel mutations: S216L, V1951L and R282H. in NEUROCARD 2014 : 6th International Symposium on Neurocardiology : The 5th International Symposium on Noninvasive Electrocardiology : Book of abstracts
Belgrade : Serbian Neuroscience Society., 57-57.
https://hdl.handle.net/21.15107/rcub_vinar_12686

Stanković A, Kolić I, Živković M. Genetic polymorphism SCN5A-H558R as potential target for future therapies of trafficking defect associated with cardiac sodium channel mutations: S216L, V1951L and R282H. in NEUROCARD 2014 : 6th International Symposium on Neurocardiology : The 5th International Symposium on Noninvasive Electrocardiology : Book of abstracts. 2014;:57-57.
https://hdl.handle.net/21.15107/rcub_vinar_12686 .

Stanković, Aleksandra, Kolić, Ivana, Živković, Maja, "Genetic polymorphism SCN5A-H558R as potential target for future therapies of trafficking defect associated with cardiac sodium channel mutations: S216L, V1951L and R282H" in NEUROCARD 2014 : 6th International Symposium on Neurocardiology : The 5th International Symposium on Noninvasive Electrocardiology : Book of abstracts (2014):57-57,
https://hdl.handle.net/21.15107/rcub_vinar_12686 .

TY  - CONF
AU  - Kolić, Ivana
AU  - Jovanović, Ivan
AU  - Bojić Milinović, Tijana
AU  - Milovanović, Branislav
AU  - Đurić, Tamara
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12687
PB  - Belgrade : Serbian Neuroscience Society
C3  - NEUROCARD 2014 : 6th International Symposium on Neurocardiology : The 5th International Symposium on Noninvasive Electrocardiology : Book of abstracts
T1  - Renin-angiotensin system gene polymorphisms in association with cardiovascular profiles in patients with vasovagal syncope
SP  - 58
EP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12687
ER  - 

@conference{
author = "Kolić, Ivana and Jovanović, Ivan and Bojić Milinović, Tijana and Milovanović, Branislav and Đurić, Tamara and Alavantić, Dragan and Stanković, Aleksandra",
year = "2014",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "NEUROCARD 2014 : 6th International Symposium on Neurocardiology : The 5th International Symposium on Noninvasive Electrocardiology : Book of abstracts",
title = "Renin-angiotensin system gene polymorphisms in association with cardiovascular profiles in patients with vasovagal syncope",
pages = "58-58",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12687"
}

Kolić, I., Jovanović, I., Bojić Milinović, T., Milovanović, B., Đurić, T., Alavantić, D.,& Stanković, A.. (2014). Renin-angiotensin system gene polymorphisms in association with cardiovascular profiles in patients with vasovagal syncope. in NEUROCARD 2014 : 6th International Symposium on Neurocardiology : The 5th International Symposium on Noninvasive Electrocardiology : Book of abstracts
Belgrade : Serbian Neuroscience Society., 58-58.
https://hdl.handle.net/21.15107/rcub_vinar_12687

Kolić I, Jovanović I, Bojić Milinović T, Milovanović B, Đurić T, Alavantić D, Stanković A. Renin-angiotensin system gene polymorphisms in association with cardiovascular profiles in patients with vasovagal syncope. in NEUROCARD 2014 : 6th International Symposium on Neurocardiology : The 5th International Symposium on Noninvasive Electrocardiology : Book of abstracts. 2014;:58-58.
https://hdl.handle.net/21.15107/rcub_vinar_12687 .

Kolić, Ivana, Jovanović, Ivan, Bojić Milinović, Tijana, Milovanović, Branislav, Đurić, Tamara, Alavantić, Dragan, Stanković, Aleksandra, "Renin-angiotensin system gene polymorphisms in association with cardiovascular profiles in patients with vasovagal syncope" in NEUROCARD 2014 : 6th International Symposium on Neurocardiology : The 5th International Symposium on Noninvasive Electrocardiology : Book of abstracts (2014):58-58,
https://hdl.handle.net/21.15107/rcub_vinar_12687 .

TY  - CONF
AU  - Životić, Ivan
AU  - Kolić, Ivana
AU  - Milovanović, Branislav
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Stanković, Aleksandra
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12688
PB  - Belgrade : Serbian Neuroscience Society
C3  - NEUROCARD 2014 : 6th International Symposium on Neurocardiology : The 5th International Symposium on Noninvasive Electrocardiology : Book of abstracts
T1  - The impact of RAS genes and BDKRB2 −58C/T (rs1799722) gene polymorphism on electrocardiographic and heart rate variability in hypertension
SP  - 59
EP  - 59
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12688
ER  - 

@conference{
author = "Životić, Ivan and Kolić, Ivana and Milovanović, Branislav and Đurić, Tamara and Živković, Maja and Stanković, Aleksandra",
year = "2014",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "NEUROCARD 2014 : 6th International Symposium on Neurocardiology : The 5th International Symposium on Noninvasive Electrocardiology : Book of abstracts",
title = "The impact of RAS genes and BDKRB2 −58C/T (rs1799722) gene polymorphism on electrocardiographic and heart rate variability in hypertension",
pages = "59-59",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12688"
}

Životić, I., Kolić, I., Milovanović, B., Đurić, T., Živković, M.,& Stanković, A.. (2014). The impact of RAS genes and BDKRB2 −58C/T (rs1799722) gene polymorphism on electrocardiographic and heart rate variability in hypertension. in NEUROCARD 2014 : 6th International Symposium on Neurocardiology : The 5th International Symposium on Noninvasive Electrocardiology : Book of abstracts
Belgrade : Serbian Neuroscience Society., 59-59.
https://hdl.handle.net/21.15107/rcub_vinar_12688

Životić I, Kolić I, Milovanović B, Đurić T, Živković M, Stanković A. The impact of RAS genes and BDKRB2 −58C/T (rs1799722) gene polymorphism on electrocardiographic and heart rate variability in hypertension. in NEUROCARD 2014 : 6th International Symposium on Neurocardiology : The 5th International Symposium on Noninvasive Electrocardiology : Book of abstracts. 2014;:59-59.
https://hdl.handle.net/21.15107/rcub_vinar_12688 .

Životić, Ivan, Kolić, Ivana, Milovanović, Branislav, Đurić, Tamara, Živković, Maja, Stanković, Aleksandra, "The impact of RAS genes and BDKRB2 −58C/T (rs1799722) gene polymorphism on electrocardiographic and heart rate variability in hypertension" in NEUROCARD 2014 : 6th International Symposium on Neurocardiology : The 5th International Symposium on Noninvasive Electrocardiology : Book of abstracts (2014):59-59,
https://hdl.handle.net/21.15107/rcub_vinar_12688 .

TY  - CONF
AU  - Stanković, Aleksandra
AU  - Bojić Milinović, Tijana
AU  - Milovanović, Branislav
AU  - Kolić, Ivana
AU  - Jovanović, Ivan
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12685
PB  - Belgrade : Serbian Neuroscience Society
C3  - NEUROCARD 2013 : 5th International Symposium on Neurocardiology : The 4th International Symposium on Noninvasive Electrocardiology : Book of abstracts
T1  - The ATR1 1166A/C and BDKRB2 −58C/T (rs1799722) gene polymorphisms impact on electrocardiographic and heart rate variability in hypertensive Serbian patients. Preliminary study
SP  - 82
EP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12685
ER  - 

@conference{
author = "Stanković, Aleksandra and Bojić Milinović, Tijana and Milovanović, Branislav and Kolić, Ivana and Jovanović, Ivan and Alavantić, Dragan and Živković, Maja",
year = "2013",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "NEUROCARD 2013 : 5th International Symposium on Neurocardiology : The 4th International Symposium on Noninvasive Electrocardiology : Book of abstracts",
title = "The ATR1 1166A/C and BDKRB2 −58C/T (rs1799722) gene polymorphisms impact on electrocardiographic and heart rate variability in hypertensive Serbian patients. Preliminary study",
pages = "82-82",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12685"
}

Stanković, A., Bojić Milinović, T., Milovanović, B., Kolić, I., Jovanović, I., Alavantić, D.,& Živković, M.. (2013). The ATR1 1166A/C and BDKRB2 −58C/T (rs1799722) gene polymorphisms impact on electrocardiographic and heart rate variability in hypertensive Serbian patients. Preliminary study. in NEUROCARD 2013 : 5th International Symposium on Neurocardiology : The 4th International Symposium on Noninvasive Electrocardiology : Book of abstracts
Belgrade : Serbian Neuroscience Society., 82-82.
https://hdl.handle.net/21.15107/rcub_vinar_12685

Stanković A, Bojić Milinović T, Milovanović B, Kolić I, Jovanović I, Alavantić D, Živković M. The ATR1 1166A/C and BDKRB2 −58C/T (rs1799722) gene polymorphisms impact on electrocardiographic and heart rate variability in hypertensive Serbian patients. Preliminary study. in NEUROCARD 2013 : 5th International Symposium on Neurocardiology : The 4th International Symposium on Noninvasive Electrocardiology : Book of abstracts. 2013;:82-82.
https://hdl.handle.net/21.15107/rcub_vinar_12685 .

Stanković, Aleksandra, Bojić Milinović, Tijana, Milovanović, Branislav, Kolić, Ivana, Jovanović, Ivan, Alavantić, Dragan, Živković, Maja, "The ATR1 1166A/C and BDKRB2 −58C/T (rs1799722) gene polymorphisms impact on electrocardiographic and heart rate variability in hypertensive Serbian patients. Preliminary study" in NEUROCARD 2013 : 5th International Symposium on Neurocardiology : The 4th International Symposium on Noninvasive Electrocardiology : Book of abstracts (2013):82-82,
https://hdl.handle.net/21.15107/rcub_vinar_12685 .